Rh2AG306700

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002948
Physical Location & Seq
Forward (+)
38640211 .. 38644031
3821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG306700.1

Sequence Viewer

Length: 1080 bp
ATGATCATGGAGAGAAGAAGGAGATGGTTGCTGTTGTTGGCACTACTTACTTGCCTCTTTGCTTCTTCTGCTGGGAGAGAATGGAAGTTCAAGCACAAGGTTCATAGCCAGAGCCCTGTCTACAATCATACGCTTGCCACAATATTGGTTCGCTATGCCTCTACAGTGTATTTGTCAGATTTGACAGAGCTGTTTTCTTGGACATGCGACAGATGTGATGGCTTGATTAAGGATTTTCAAATGATAGAGATAATTGTGGACGTCCAGCATTGCTTACAGGCATTTGTTGGGGTGGCAACAGATCCAAATGCTATTATAATTGCATTTAAAGGGACTCAGGGAAACAGTATACAGAATTGGATCGAAGACTTATTTTGGAAACAACTTGATATAGATTACCCTGGCATGCCTGATGCAATGGTGCACCATGGTTTTTATAATGCTTACCACAACACAACCATACGACCTGGAATTCTAAATGCTGTTGCAAGAGCCAAAGAGTTTTATGGAGATATTGGCATCATAGTGACAGGGCATTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATTTAAGGGTTAATCAAAAGGAGAATAATGTTCAAGTTATAACATTTGGACAACCTCGAATCGGTAATGCAATTTTTGCTACTTACTATAGTGAAATTCTGCCACAGACTATACGAGTAACAAATGGAAATGATGTTGTGCCTCATTTGCCTCCATACTACACTTATTTTCCTCAGAAGACATACCACCACTTCCCAACAGAGGTGTGGCTTTATAACATTGGATTGCGAAGTCTTGTTTATCAGGTTGAGAAAATATGTGATGCCTCTGGTGAGGACCCAACCTGCAGCAGGTCAGTGATAGGGGACAGCATTTCAGACCATTTAGTTTATTATGGTGTCAAATTAATGGATGAGACATGGAGACCGTGCAAAATTGTGACGGGTCCTGGTTTACTGGATCATGGAAGATCAGATCTGGAAGGAAACTTTGTTCTGTCCAGAGATCTTGCAACTCCTGTTCTCAAATTGAAAATACAATCAGATGCTGGGGGAAAGCCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

359

Amino Acids

40.51

Weight (kDa)

6.49

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 106 - 244 1.3e-35 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 317, 438, 614, 789
AatII GACGTC 1 cut(s) 264
Acc36I ACCTGC 2 cut(s) 855, 866
AccI GTMKAC 2 cut(s) 120, 349
AclWI GGATC 3 cut(s) 296, 368, 981
AcsI RAATTY 2 cut(s) 471, 669
AcyI GRCGYC 1 cut(s) 261
AfiI CCNNNNNNNGG 3 cut(s) 635, 775, 864
AgsI TTSAA 5 cut(s) 91, 239, 540, 608, 1045
AjnI CCWGG 3 cut(s) 400, 466, 961
AloI GAACNNNNNNTCC 2 cut(s) 990, 1022
AluBI AGCT 1 cut(s) 190
AluI AGCT 1 cut(s) 190
Alw21I GWGCWC 2 cut(s) 426, 571
Alw26I GTCTC 2 cut(s) 923, 931
Alw44I GTGCAC 1 cut(s) 422
AlwI GGATC 3 cut(s) 296, 368, 981
AlwNI CAGNNNCTG 1 cut(s) 1061
ApaLI GTGCAC 1 cut(s) 422
ApeKI GCWGC 1 cut(s) 861
ApoI RAATTY 2 cut(s) 471, 669
AseI ATTAAT 1 cut(s) 920
AspS9I GGNCC 2 cut(s) 850, 959
AsuHPI GGTGA 1 cut(s) 857
AvaII GGWCC 2 cut(s) 850, 959
BaeGI GKGCMC 1 cut(s) 426
BanII GRGCYC 1 cut(s) 116
BbsI GAAGAC 2 cut(s) 372, 758
Bbv12I GWGCWC 2 cut(s) 426, 571
BbvI GCAGC 1 cut(s) 873
BccI CCATC 2 cut(s) 18, 212
BciT130I CCWGG 3 cut(s) 402, 468, 963
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 2 cut(s) 923, 931
BfmI CTRYAG 4 cut(s) 162, 661, 859, 1076
BfuAI ACCTGC 2 cut(s) 855, 866
BglII AGATCT 2 cut(s) 988, 1018
BisI GCNGC 1 cut(s) 862
BlsI GCNGC 1 cut(s) 863
Bme1390I CCNGG 3 cut(s) 402, 468, 963
Bme18I GGWCC 2 cut(s) 850, 959
BmgT120I GGNCC 2 cut(s) 850, 959
BmiI GGNNCC 2 cut(s) 852, 960
BmrFI CCNGG 3 cut(s) 402, 468, 963
BmsI GCATC 4 cut(s) 403, 528, 826, 1048
BpiI GAAGAC 2 cut(s) 372, 758
BsaHI GRCGYC 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 931
BsaJI CCNNGG 2 cut(s) 400, 427
Bsc4I CCNNNNNNNGG 3 cut(s) 635, 775, 864
Bse1I ACTGG 1 cut(s) 975
Bse3DI GCAATG 3 cut(s) 268, 423, 558
BseBI CCWGG 3 cut(s) 402, 468, 963
BseDI CCNNGG 2 cut(s) 400, 427
BseGI GGATG 1 cut(s) 931
BseLI CCNNNNNNNGG 3 cut(s) 635, 775, 864
BseMI GCAATG 3 cut(s) 268, 423, 558
BseMII CTCAG 2 cut(s) 350, 761
BseNI ACTGG 1 cut(s) 975
BseSI GKGCMC 1 cut(s) 426
BseXI GCAGC 1 cut(s) 873
BseYI CCCAGC 2 cut(s) 71, 1061
BsiHKAI GWGCWC 2 cut(s) 426, 571
BslFI GGGAC 2 cut(s) 346, 893
BslI CCNNNNNNNGG 3 cut(s) 635, 775, 864
BsmAI GTCTC 2 cut(s) 923, 931
BsmFI GGGAC 2 cut(s) 346, 893
BsmI GAATGC 1 cut(s) 535
Bso31I GGTCTC 1 cut(s) 931
Bsp1286I GDGCHC 3 cut(s) 116, 426, 571
Bsp143I GATC 7 cut(s) 3, 301, 360, 973, 983, 988, 1018
Bsp19I CCATGG 1 cut(s) 427
BspCNI CTCAG 2 cut(s) 349, 760
BspLI GGNNCC 2 cut(s) 852, 960
BspMAI CTGCAG 1 cut(s) 863
BspMI ACCTGC 2 cut(s) 855, 866
BspPI GGATC 3 cut(s) 296, 368, 981
BspTNI GGTCTC 1 cut(s) 931
BsrDI GCAATG 3 cut(s) 268, 423, 558
BsrI ACTGG 1 cut(s) 975
BssECI CCNNGG 2 cut(s) 400, 427
BssMI GATC 7 cut(s) 3, 301, 360, 973, 983, 988, 1018
BssNAI GTATAC 1 cut(s) 350
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 427
Bst1107I GTATAC 1 cut(s) 350
Bst2UI CCWGG 3 cut(s) 402, 468, 963
Bst4CI ACNGT 3 cut(s) 166, 347, 942
BstACI GRCGYC 1 cut(s) 261
BstAPI GCANNNNNTGC 1 cut(s) 650
BstC8I GCNNGC 2 cut(s) 135, 407
BstDEI CTNAG 2 cut(s) 336, 747
BstDSI CCRYGG 1 cut(s) 427
BstENI CCTNNNNNAGG 1 cut(s) 862
BstF5I GGATG 1 cut(s) 931
BstKTI GATC 7 cut(s) 6, 304, 363, 976, 986, 991, 1021
BstMAI GTCTC 2 cut(s) 923, 931
BstMBI GATC 7 cut(s) 3, 301, 360, 973, 983, 988, 1018
BstMWI GCNNNNNNNGC 3 cut(s) 68, 650, 721
BstNI CCWGG 3 cut(s) 402, 468, 963
BstNSI RCATGY 2 cut(s) 207, 409
BstSCI CCNGG 3 cut(s) 400, 466, 961
BstSFI CTRYAG 4 cut(s) 162, 661, 859, 1076
BstSLI GKGCMC 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 873
BstV2I GAAGAC 2 cut(s) 372, 758
BstX2I RGATCY 3 cut(s) 301, 988, 1018
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 3 cut(s) 301, 988, 1018
BstZ17I GTATAC 1 cut(s) 350
BtgI CCRYGG 1 cut(s) 427
BtsCI GGATG 1 cut(s) 931
BtsIMutI CAGTG 2 cut(s) 171, 876
BveI ACCTGC 2 cut(s) 855, 866
Cac8I GCNNGC 2 cut(s) 135, 407
CaiI CAGNNNCTG 1 cut(s) 1061
Cfr13I GGNCC 2 cut(s) 850, 959
CviAII CATG 6 cut(s) 7, 204, 406, 428, 933, 977
CviJI RGCY 8 cut(s) 108, 114, 190, 222, 494, 557, 784, 1072
CviKI_1 RGCY 8 cut(s) 108, 114, 190, 222, 494, 557, 784, 1072
DdeI CTNAG 2 cut(s) 336, 747
DpnI GATC 7 cut(s) 5, 303, 362, 975, 985, 990, 1020
DpnII GATC 7 cut(s) 3, 301, 360, 973, 983, 988, 1018
DraI TTTAAA 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 427
Eco24I GRGCYC 1 cut(s) 116
Eco31I GGTCTC 1 cut(s) 931
Eco47I GGWCC 2 cut(s) 850, 959
EcoNI CCTNNNNNAGG 1 cut(s) 862
EcoO109I RGGNCCY 2 cut(s) 850, 959
EcoRI GAATTC 1 cut(s) 471
EcoRII CCWGG 3 cut(s) 400, 466, 961
EcoT14I CCWWGG 1 cut(s) 427
EcoT38I GRGCYC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 6 cut(s) 10, 207, 409, 431, 936, 980
FaqI GGGAC 2 cut(s) 346, 893
FatI CATG 6 cut(s) 6, 203, 405, 427, 932, 976
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 120, 349
Fnu4HI GCNGC 1 cut(s) 862
FokI GGATG 1 cut(s) 938
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 1 cut(s) 862
GluI GCNGC 1 cut(s) 862
GsaI CCCAGC 2 cut(s) 75, 1065
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 6 cut(s) 10, 207, 409, 431, 936, 980
HinfI GANTC 2 cut(s) 334, 633
HphI GGTGA 1 cut(s) 857
Hpy166II GTNNAC 5 cut(s) 121, 259, 350, 424, 968
Hpy188I TCNGA 5 cut(s) 178, 750, 892, 988, 1057
Hpy188III TCNNGA 3 cut(s) 572, 992, 1014
Hpy8I GTNNAC 5 cut(s) 121, 259, 350, 424, 968
HpyAV CCTTC 2 cut(s) 12, 989
HpyCH4III ACNGT 3 cut(s) 166, 347, 942
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 8 cut(s) 323, 416, 424, 488, 644, 861, 945, 1025
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 650, 721
HpyF3I CTNAG 2 cut(s) 336, 747
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 6 cut(s) 10, 207, 409, 431, 936, 980
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 7 cut(s) 3, 301, 360, 973, 983, 988, 1018
Lsp1109I GCAGC 1 cut(s) 873
LweI GCATC 4 cut(s) 403, 528, 826, 1048
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 526, 691, 952
MalI GATC 7 cut(s) 5, 303, 362, 975, 985, 990, 1020
MboI GATC 7 cut(s) 3, 301, 360, 973, 983, 988, 1018
MboII GAAGA 5 cut(s) 27, 57, 377, 763, 993
MflI RGATCY 3 cut(s) 301, 988, 1018
MhlI GDGCHC 3 cut(s) 116, 426, 571
MluCI AATT 9 cut(s) 252, 318, 355, 471, 645, 669, 917, 948, 1040
MlyI GAGTC 1 cut(s) 328
MseI TTAA 5 cut(s) 228, 327, 578, 585, 920
MslI CAYNNNNRTG 1 cut(s) 524
MspR9I CCNGG 3 cut(s) 402, 468, 963
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 3 cut(s) 402, 468, 963
MwoI GCNNNNNNNGC 3 cut(s) 68, 650, 721
NcoI CCATGG 1 cut(s) 427
NdeII GATC 7 cut(s) 3, 301, 360, 973, 983, 988, 1018
NlaIII CATG 6 cut(s) 10, 207, 409, 431, 936, 980
NlaIV GGNNCC 2 cut(s) 852, 960
NmuCI GTSAC 2 cut(s) 526, 952
NspI RCATGY 2 cut(s) 207, 409
PaeI GCATGC 1 cut(s) 409
PctI GAATGC 1 cut(s) 535
PfeI GAWTC 1 cut(s) 633
PkrI GCNGC 1 cut(s) 863
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
PpuMI RGGWCCY 2 cut(s) 850, 959
PshBI ATTAAT 1 cut(s) 920
PsiI TTATAA 4 cut(s) 317, 438, 614, 789
Psp5II RGGWCCY 2 cut(s) 850, 959
Psp6I CCWGG 3 cut(s) 400, 466, 961
PspFI CCCAGC 2 cut(s) 71, 1061
PspGI CCWGG 3 cut(s) 400, 466, 961
PspN4I GGNNCC 2 cut(s) 852, 960
PspPI GGNCC 2 cut(s) 850, 959
PspPPI RGGWCCY 2 cut(s) 850, 959
PstI CTGCAG 1 cut(s) 863
PstNI CAGNNNCTG 1 cut(s) 1061
PsuI RGATCY 3 cut(s) 301, 988, 1018
RseI CAYNNNNRTG 1 cut(s) 524
SaqAI TTAA 5 cut(s) 228, 327, 578, 585, 920
SatI GCNGC 1 cut(s) 862
Sau3AI GATC 7 cut(s) 3, 301, 360, 973, 983, 988, 1018
Sau96I GGNCC 2 cut(s) 850, 959
SchI GAGTC 1 cut(s) 328
ScrFI CCNGG 3 cut(s) 402, 468, 963
SduI GDGCHC 3 cut(s) 116, 426, 571
SetI ASST 9 cut(s) 102, 192, 264, 469, 631, 780, 822, 860, 869
SfaNI GCATC 4 cut(s) 403, 528, 826, 1048
SfcI CTRYAG 4 cut(s) 162, 661, 859, 1076
SinI GGWCC 2 cut(s) 850, 959
SmiMI CAYNNNNRTG 1 cut(s) 524
SphI GCATGC 1 cut(s) 409
Sse9I AATT 9 cut(s) 252, 318, 355, 471, 645, 669, 917, 948, 1040
SspI AATATT 1 cut(s) 144
StyD4I CCNGG 3 cut(s) 400, 466, 961
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 3 cut(s) 166, 347, 942
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 363, 631
TasI AATT 9 cut(s) 252, 318, 355, 471, 645, 669, 917, 948, 1040
TfiI GAWTC 1 cut(s) 633
Tru1I TTAA 5 cut(s) 228, 327, 578, 585, 920
Tru9I TTAA 5 cut(s) 228, 327, 578, 585, 920
TscAI CASTG 2 cut(s) 171, 876
TseFI GTSAC 2 cut(s) 526, 952
TseI GCWGC 1 cut(s) 861
Tsp45I GTSAC 2 cut(s) 526, 952
TspDTI ATGAA 2 cut(s) 92, 549
TspRI CASTG 2 cut(s) 171, 876
VneI GTGCAC 1 cut(s) 422
VpaK11BI GGWCC 2 cut(s) 850, 959
VspI ATTAAT 1 cut(s) 920
XagI CCTNNNNNAGG 1 cut(s) 862
XapI RAATTY 2 cut(s) 471, 669
XceI RCATGY 2 cut(s) 207, 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 777
XmiI GTMKAC 2 cut(s) 120, 349
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.