MD13G1172600.v1.1

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002948
Physical Location & Seq
Forward (+)
14164517 .. 14168713
4197 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1172600.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGTTTGTTTTCTCTGCTGGGAGAGAACTGAAAATTAAGCACAACGATTGTGGCCCCATATACAATCATACGCTTGCCACTATACTAGTGCGATATGCTTCTACAGTTTATTTGTCAGATTTGTCAGAGCTGTTTACTTGGACATGCTCATGCTGTAATGATTTGATTAAGGGGTTTGAAATGATAGAGATTATTGTTGATGTCCAGCATTGCTTACAGGCTTTTGTTGGAGTGGCACAAGATCCCAATGCTATTATAATTGCATTTAGAGGGACTCAGGAACACAGCATACAGAATTGGATTGAAGACTTATTTTGGAAACAACTTGATCTAGATTACCCTGGCATGCCTGATGCAATGGTGCACCACGGGTTTTATAGTGCTTACCACAACACAACCATACGCCCTGGAATTCTAAATGCTGTGGAAAGAGCCAGGGAATATTATGGAGATATCGGCATCATAGTTACAGGACATTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATCTAAGGGTCAATCAGAAAGAGAAAAATGTTCAAGTTATGACATTTGGACAACCTCGTATCGGTAATGCAGCTTTTGCATCATACTATAGTACACTTGTACCAAATAGTATTCGAGTAACAAATGCACATGATATGGTGCCTCATTTACCTCCATACTATACTTATTTTCCTCAGAAGACATACCACCACTTTCCACGAGAGGTGTGGCTTTATAACCTTGAAGTTGAAAGTCTTGTTTATGAAGTTGAGAAAATCTGTGATGATTCAGGCGAGGACCCAACGTGCAGCAGGTCAGTGAGTGGGAACAGCATTTCGGACCATTTAGTTTATTATGGTGTTGACTTAATGGCCAAGACATGGAGACGGTGCAAAATTGTGATGGGTACTGGTGTAGCAGAGTACAGCAGAACAGATCTTGGAGGAAATTTTATACTGTCCAGAGATCCTGCAACTTCTGTTCTCAAGTTGAACGTAGCGCCAGACACTGGGGCAAAACCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

340

Amino Acids

38.15

Weight (kDa)

5.78

Isoelectric Point (pI)

26.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 86 - 224 4.4e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 257, 729
Acc36I ACCTGC 1 cut(s) 795
AccB1I GGYRCC 1 cut(s) 652
AccB7I CCANNNNNTGG 2 cut(s) 873, 1001
AclWI GGATC 2 cut(s) 236, 953
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 2 cut(s) 411, 940
AfaI GTAC 4 cut(s) 607, 615, 901, 917
AfiI CCNNNNNNNGG 3 cut(s) 575, 873, 1001
AgsI TTSAA 7 cut(s) 179, 305, 480, 548, 737, 743, 985
AhlI ACTAGT 1 cut(s) 85
AjnI CCWGG 3 cut(s) 340, 406, 434
AluBI AGCT 2 cut(s) 130, 587
AluI AGCT 2 cut(s) 130, 587
Alw21I GWGCWC 2 cut(s) 366, 511
Alw26I GTCTC 1 cut(s) 871
Alw44I GTGCAC 1 cut(s) 362
AlwI GGATC 2 cut(s) 236, 953
AlwNI CAGNNNCTG 1 cut(s) 1001
AoxI GGCC 2 cut(s) 52, 864
ApaLI GTGCAC 1 cut(s) 362
ApeKI GCWGC 2 cut(s) 584, 801
ApoI RAATTY 2 cut(s) 411, 940
AspLEI GCGC 1 cut(s) 994
AspS9I GGNCC 3 cut(s) 53, 790, 832
AvaII GGWCC 2 cut(s) 790, 832
BaeGI GKGCMC 1 cut(s) 366
BaeI ACNNNNGTAYC 4 cut(s) 597, 630, 891, 924
BalI TGGCCA 1 cut(s) 866
BanI GGYRCC 1 cut(s) 652
BauI CACGAG 1 cut(s) 711
BbsI GAAGAC 2 cut(s) 312, 698
Bbv12I GWGCWC 2 cut(s) 366, 511
BbvI GCAGC 2 cut(s) 596, 813
BccI CCATC 1 cut(s) 889
BciT130I CCWGG 3 cut(s) 342, 408, 436
BcoDI GTCTC 1 cut(s) 871
BcuI ACTAGT 1 cut(s) 85
BfaI CTAG 2 cut(s) 86, 332
BfmI CTRYAG 3 cut(s) 102, 601, 1016
BfoI RGCGCY 1 cut(s) 995
BfuAI ACCTGC 1 cut(s) 795
BglII AGATCT 1 cut(s) 928
BisI GCNGC 2 cut(s) 585, 802
BlsI GCNGC 2 cut(s) 586, 803
Bme1390I CCNGG 3 cut(s) 342, 408, 436
Bme18I GGWCC 2 cut(s) 790, 832
BmgT120I GGNCC 3 cut(s) 53, 790, 832
BmiI GGNNCC 3 cut(s) 55, 654, 792
BmrFI CCNGG 3 cut(s) 342, 408, 436
BmrI ACTGGG 1 cut(s) 1011
BmsI GCATC 3 cut(s) 343, 468, 602
BmuI ACTGGG 1 cut(s) 1011
BpiI GAAGAC 2 cut(s) 312, 698
BpuEI CTTGAG 1 cut(s) 962
BsaJI CCNNGG 4 cut(s) 340, 367, 406, 435
Bsc4I CCNNNNNNNGG 3 cut(s) 575, 873, 1001
Bse1I ACTGG 2 cut(s) 907, 1006
Bse3DI GCAATG 3 cut(s) 208, 363, 498
BseBI CCWGG 3 cut(s) 342, 408, 436
BseDI CCNNGG 4 cut(s) 340, 367, 406, 435
BseLI CCNNNNNNNGG 3 cut(s) 575, 873, 1001
BseMI GCAATG 3 cut(s) 208, 363, 498
BseMII CTCAG 2 cut(s) 290, 701
BseNI ACTGG 2 cut(s) 907, 1006
BseSI GKGCMC 1 cut(s) 366
BseXI GCAGC 2 cut(s) 596, 813
BseYI CCCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 820
BshFI GGCC 2 cut(s) 54, 866
BshNI GGYRCC 1 cut(s) 652
BsiHKAI GWGCWC 2 cut(s) 366, 511
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 3 cut(s) 575, 873, 1001
BsmAI GTCTC 1 cut(s) 871
BsmBI CGTCTC 1 cut(s) 871
BsmFI GGGAC 1 cut(s) 286
BsnI GGCC 2 cut(s) 54, 866
Bsp1286I GDGCHC 2 cut(s) 366, 511
Bsp143I GATC 5 cut(s) 241, 328, 514, 928, 958
BspANI GGCC 2 cut(s) 54, 866
BspCNI CTCAG 2 cut(s) 289, 700
BspLI GGNNCC 3 cut(s) 55, 654, 792
BspMI ACCTGC 1 cut(s) 795
BspPI GGATC 2 cut(s) 236, 953
BspT107I GGYRCC 1 cut(s) 652
BsrDI GCAATG 3 cut(s) 208, 363, 498
BsrI ACTGG 2 cut(s) 907, 1006
BssECI CCNNGG 4 cut(s) 340, 367, 406, 435
BssMI GATC 5 cut(s) 241, 328, 514, 928, 958
BssSI CACGAG 1 cut(s) 711
Bst2BI CACGAG 1 cut(s) 711
Bst2UI CCWGG 3 cut(s) 342, 408, 436
Bst4CI ACNGT 3 cut(s) 106, 882, 951
BstAPI GCANNNNNTGC 1 cut(s) 590
BstC8I GCNNGC 2 cut(s) 75, 347
BstDEI CTNAG 3 cut(s) 276, 518, 687
BstDSI CCRYGG 1 cut(s) 367
BstH2I RGCGCY 1 cut(s) 995
BstHHI GCGC 1 cut(s) 994
BstKTI GATC 5 cut(s) 244, 331, 517, 931, 961
BstMAI GTCTC 1 cut(s) 871
BstMBI GATC 5 cut(s) 241, 328, 514, 928, 958
BstMWI GCNNNNNNNGC 1 cut(s) 590
BstNI CCWGG 3 cut(s) 342, 408, 436
BstNSI RCATGY 2 cut(s) 147, 349
BstSCI CCNGG 3 cut(s) 340, 406, 434
BstSFI CTRYAG 3 cut(s) 102, 601, 1016
BstSLI GKGCMC 1 cut(s) 366
BstV1I GCAGC 2 cut(s) 596, 813
BstV2I GAAGAC 2 cut(s) 312, 698
BstX2I RGATCY 3 cut(s) 241, 928, 958
BstYI RGATCY 3 cut(s) 241, 928, 958
BsuRI GGCC 2 cut(s) 54, 866
BtgI CCRYGG 1 cut(s) 367
BtsIMutI CAGTG 2 cut(s) 816, 999
BveI ACCTGC 1 cut(s) 795
Cac8I GCNNGC 2 cut(s) 75, 347
CaiI CAGNNNCTG 1 cut(s) 1001
CfoI GCGC 1 cut(s) 994
Cfr13I GGNCC 3 cut(s) 53, 790, 832
Csp6I GTAC 4 cut(s) 606, 614, 900, 916
CspCI CAANNNNNGTGG 2 cut(s) 31, 66
CviAII CATG 5 cut(s) 144, 150, 346, 644, 873
CviJI RGCY 8 cut(s) 54, 130, 221, 434, 497, 587, 724, 866
CviKI_1 RGCY 8 cut(s) 54, 130, 221, 434, 497, 587, 724, 866
CviQI GTAC 4 cut(s) 606, 614, 900, 916
DdeI CTNAG 3 cut(s) 276, 518, 687
DpnI GATC 5 cut(s) 243, 330, 516, 930, 960
DpnII GATC 5 cut(s) 241, 328, 514, 928, 958
EaeI YGGCCR 1 cut(s) 864
Eco32I GATATC 1 cut(s) 454
Eco47I GGWCC 2 cut(s) 790, 832
EcoO109I RGGNCCY 1 cut(s) 790
EcoRI GAATTC 1 cut(s) 411
EcoRII CCWGG 3 cut(s) 340, 406, 434
EcoRV GATATC 1 cut(s) 454
Esp3I CGTCTC 1 cut(s) 871
FaeI CATG 5 cut(s) 147, 153, 349, 647, 876
FaqI GGGAC 1 cut(s) 286
FatI CATG 5 cut(s) 143, 149, 345, 643, 872
Fnu4HI GCNGC 2 cut(s) 585, 802
Fsp4HI GCNGC 2 cut(s) 585, 802
FspBI CTAG 2 cut(s) 86, 332
GlaI GCGC 1 cut(s) 993
GluI GCNGC 2 cut(s) 585, 802
GsaI CCCAGC 1 cut(s) 21
HaeII RGCGCY 1 cut(s) 995
HaeIII GGCC 2 cut(s) 54, 866
HhaI GCGC 1 cut(s) 994
Hin1II CATG 5 cut(s) 147, 153, 349, 647, 876
Hin6I GCGC 1 cut(s) 992
HinP1I GCGC 1 cut(s) 992
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HinfI GANTC 2 cut(s) 274, 779
Hpy166II GTNNAC 4 cut(s) 135, 364, 608, 856
Hpy188I TCNGA 5 cut(s) 118, 127, 531, 690, 832
Hpy188III TCNNGA 4 cut(s) 278, 332, 512, 954
Hpy8I GTNNAC 4 cut(s) 135, 364, 608, 856
HpyCH4III ACNGT 3 cut(s) 106, 882, 951
HpyCH4IV ACGT 2 cut(s) 797, 987
HpyCH4V TGCA 9 cut(s) 263, 356, 364, 584, 593, 641, 801, 885, 965
HpyF10VI GCNNNNNNNGC 1 cut(s) 590
HpyF3I CTNAG 3 cut(s) 276, 518, 687
HpySE526I ACGT 2 cut(s) 797, 987
Hsp92II CATG 5 cut(s) 147, 153, 349, 647, 876
HspAI GCGC 1 cut(s) 992
Kzo9I GATC 5 cut(s) 241, 328, 514, 928, 958
Lsp1109I GCAGC 2 cut(s) 596, 813
LweI GCATC 3 cut(s) 343, 468, 602
MaeI CTAG 2 cut(s) 86, 332
MaeII ACGT 2 cut(s) 797, 987
MaeIII GTNAC 2 cut(s) 466, 631
MalI GATC 5 cut(s) 243, 330, 516, 930, 960
MboI GATC 5 cut(s) 241, 328, 514, 928, 958
MboII GAAGA 2 cut(s) 317, 703
MflI RGATCY 3 cut(s) 241, 928, 958
MhlI GDGCHC 2 cut(s) 366, 511
MlsI TGGCCA 1 cut(s) 866
MluCI AATT 6 cut(s) 33, 258, 295, 411, 888, 940
MluNI TGGCCA 1 cut(s) 866
MlyI GAGTC 1 cut(s) 268
MmeI TCCRAC 1 cut(s) 208
MnlI CCTC 9 cut(s) 263, 479, 579, 666, 675, 696, 709, 781, 929
Mox20I TGGCCA 1 cut(s) 866
MscI TGGCCA 1 cut(s) 866
MseI TTAA 3 cut(s) 36, 168, 860
MslI CAYNNNNRTG 1 cut(s) 148
Msp20I TGGCCA 1 cut(s) 866
MspR9I CCNGG 3 cut(s) 342, 408, 436
MvaI CCWGG 3 cut(s) 342, 408, 436
MwoI GCNNNNNNNGC 1 cut(s) 590
NdeII GATC 5 cut(s) 241, 328, 514, 928, 958
NlaIII CATG 5 cut(s) 147, 153, 349, 647, 876
NlaIV GGNNCC 3 cut(s) 55, 654, 792
NspI RCATGY 2 cut(s) 147, 349
PaeI GCATGC 1 cut(s) 349
PfeI GAWTC 1 cut(s) 779
PflMI CCANNNNNTGG 2 cut(s) 873, 1001
PkrI GCNGC 2 cut(s) 586, 803
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 790
PsiI TTATAA 2 cut(s) 257, 729
Psp5II RGGWCCY 1 cut(s) 790
Psp6I CCWGG 3 cut(s) 340, 406, 434
PspFI CCCAGC 1 cut(s) 17
PspGI CCWGG 3 cut(s) 340, 406, 434
PspN4I GGNNCC 3 cut(s) 55, 654, 792
PspPI GGNCC 3 cut(s) 53, 790, 832
PspPPI RGGWCCY 1 cut(s) 790
PsrI GAACNNNNNNTAC 2 cut(s) 273, 305
PstNI CAGNNNCTG 1 cut(s) 1001
PsuI RGATCY 3 cut(s) 241, 928, 958
RsaI GTAC 4 cut(s) 607, 615, 901, 917
RsaNI GTAC 4 cut(s) 606, 614, 900, 916
RseI CAYNNNNRTG 1 cut(s) 148
SaqAI TTAA 3 cut(s) 36, 168, 860
SatI GCNGC 2 cut(s) 585, 802
Sau3AI GATC 5 cut(s) 241, 328, 514, 928, 958
Sau96I GGNCC 3 cut(s) 53, 790, 832
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 3 cut(s) 342, 408, 436
SduI GDGCHC 2 cut(s) 366, 511
SfaNI GCATC 3 cut(s) 343, 468, 602
SfcI CTRYAG 3 cut(s) 102, 601, 1016
SinI GGWCC 2 cut(s) 790, 832
SmiMI CAYNNNNRTG 1 cut(s) 148
SmlI CTYRAG 1 cut(s) 977
SmoI CTYRAG 1 cut(s) 977
SpeI ACTAGT 1 cut(s) 85
SphI GCATGC 1 cut(s) 349
Sse9I AATT 6 cut(s) 33, 258, 295, 411, 888, 940
SspI AATATT 1 cut(s) 443
SspMI CTAG 2 cut(s) 86, 332
StyD4I CCNGG 3 cut(s) 340, 406, 434
TaaI ACNGT 3 cut(s) 106, 882, 951
TaiI ACGT 2 cut(s) 800, 990
TaqI TCGA 1 cut(s) 628
TasI AATT 6 cut(s) 33, 258, 295, 411, 888, 940
TatI WGTACW 2 cut(s) 605, 915
TfiI GAWTC 1 cut(s) 779
Tru1I TTAA 3 cut(s) 36, 168, 860
Tru9I TTAA 3 cut(s) 36, 168, 860
TscAI CASTG 2 cut(s) 816, 1006
TseI GCWGC 2 cut(s) 584, 801
TspDTI ATGAA 2 cut(s) 489, 771
TspRI CASTG 2 cut(s) 816, 1006
Van91I CCANNNNNTGG 2 cut(s) 873, 1001
VneI GTGCAC 1 cut(s) 362
VpaK11BI GGWCC 2 cut(s) 790, 832
XapI RAATTY 2 cut(s) 411, 940
XbaI TCTAGA 1 cut(s) 331
XceI RCATGY 2 cut(s) 147, 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 717
XspI CTAG 2 cut(s) 86, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.