Rh4CG244900

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002948
Physical Location & Seq
Reverse (-)
49318679 .. 49323825
5147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG244900.1

Sequence Viewer

Length: 780 bp
ATGGAGAGAAGGAGATGGTTGCTATTGCTGGCAGTACTTGCGTGCCTGTTTGGTTGTTCTGCCGGGAGAGAACTGAGATTCAAGCACAAGATTCATAGCCATAGCCCCGTCTACAATCACACTCTTGCTACAATATTGGTTCGGTATGCTTCTACGGTGTATTTGTCAGATTTGACAGAGCTGTTTTCTTGGACATGCGACAAATGTAATGGCTTGATCAAGGATTTTCAAATGATAGAGCTGATTGTTGACATCCAGCATTGTTTACAGGCATATGTTGGAGTCGCAACAGATCCTAATGCTATTATAATTGCCTTTAGAGGAACTCAGGAACACAGCATACAGAATTGGATTGAAGACTTATTTTGGAAGCAACTTGATATAGATTACCCCGGCATGCCTGATGCAATGGTGCACCATGGGTTTTACAGCGCTTACCACAACACCACCATACGCCCTGGAATTCTAAATGCTGTTGCAAGAGCCAAAGAGTTTTATGGAGATATTGGGATCATAGTGACAGGGCATTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATTTAAGGGTCAATCAAAAAGAGAACAATGTTCAAGTTATGACATTTGGACAACCTCGTATCGGTAATGCAATCTTTGCTACTTACTATAGCAAACTTGTGCCAAAAAGTATACGAATAACAAATGGAAATGATGTTGTGCCTCATTTGCCTCCATACTATACTCACTTTCCTCAGAAGACATACCACCACTTCCCAAGAGAGGTATTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

259

Amino Acids

29.6

Weight (kDa)

8.19

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 103 - 241 1.8e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 308
AccI GTMKAC 2 cut(s) 111, 676
AclWI GGATC 2 cut(s) 287, 518
AcsI RAATTY 1 cut(s) 462
AfaI GTAC 1 cut(s) 36
AfeI AGCGCT 1 cut(s) 433
AfiI CCNNNNNNNGG 2 cut(s) 626, 766
AgsI TTSAA 5 cut(s) 82, 230, 356, 531, 599
AjnI CCWGG 1 cut(s) 457
AluBI AGCT 2 cut(s) 181, 241
AluI AGCT 2 cut(s) 181, 241
Alw21I GWGCWC 2 cut(s) 417, 562
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 2 cut(s) 287, 518
Aor51HI AGCGCT 1 cut(s) 433
ApaLI GTGCAC 1 cut(s) 413
ApoI RAATTY 1 cut(s) 462
AspLEI GCGC 1 cut(s) 434
AsuC2I CCSGG 2 cut(s) 64, 393
BaeGI GKGCMC 1 cut(s) 417
BbsI GAAGAC 2 cut(s) 363, 749
Bbv12I GWGCWC 2 cut(s) 417, 562
BccI CCATC 1 cut(s) 9
BciT130I CCWGG 1 cut(s) 459
BclI TGATCA 1 cut(s) 216
BcnI CCSGG 2 cut(s) 64, 393
BfmI CTRYAG 1 cut(s) 652
BfoI RGCGCY 1 cut(s) 435
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 3 cut(s) 64, 393, 459
BmrFI CCNGG 3 cut(s) 64, 393, 459
BmsI GCATC 1 cut(s) 394
BpiI GAAGAC 2 cut(s) 363, 749
BpuMI CCSGG 2 cut(s) 64, 393
BsaJI CCNNGG 3 cut(s) 391, 418, 457
Bsc4I CCNNNNNNNGG 2 cut(s) 626, 766
Bse3DI GCAATG 2 cut(s) 414, 549
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 3 cut(s) 391, 418, 457
BseGI GGATG 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 626, 766
BseMI GCAATG 2 cut(s) 414, 549
BseMII CTCAG 3 cut(s) 65, 341, 752
BseSI GKGCMC 1 cut(s) 417
BsiHKAI GWGCWC 2 cut(s) 417, 562
BsiSI CCGG 2 cut(s) 63, 393
BslI CCNNNNNNNGG 2 cut(s) 626, 766
BsmI GAATGC 1 cut(s) 526
Bsp1286I GDGCHC 2 cut(s) 417, 562
Bsp143I GATC 3 cut(s) 216, 292, 510
Bsp19I CCATGG 1 cut(s) 418
BspCNI CTCAG 3 cut(s) 66, 340, 751
BspPI GGATC 2 cut(s) 287, 518
BsrDI GCAATG 2 cut(s) 414, 549
BssECI CCNNGG 3 cut(s) 391, 418, 457
BssMI GATC 3 cut(s) 216, 292, 510
BssNAI GTATAC 1 cut(s) 677
BssT1I CCWWGG 1 cut(s) 418
Bst1107I GTATAC 1 cut(s) 677
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 157
BstAPI GCANNNNNTGC 2 cut(s) 38, 641
BstC8I GCNNGC 3 cut(s) 30, 43, 398
BstDEI CTNAG 3 cut(s) 74, 327, 738
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 1 cut(s) 252
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 3 cut(s) 219, 295, 513
BstMBI GATC 3 cut(s) 216, 292, 510
BstMWI GCNNNNNNNGC 3 cut(s) 38, 641, 712
BstNI CCWGG 1 cut(s) 459
BstNSI RCATGY 2 cut(s) 198, 400
BstSCI CCNGG 3 cut(s) 62, 391, 457
BstSFI CTRYAG 1 cut(s) 652
BstSLI GKGCMC 1 cut(s) 417
BstV2I GAAGAC 2 cut(s) 363, 749
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BstZ17I GTATAC 1 cut(s) 677
BtgI CCRYGG 1 cut(s) 418
BtsCI GGATG 1 cut(s) 252
Cac8I GCNNGC 3 cut(s) 30, 43, 398
CfoI GCGC 1 cut(s) 434
Csp6I GTAC 1 cut(s) 35
CviAII CATG 3 cut(s) 195, 397, 419
CviJI RGCY 7 cut(s) 99, 105, 181, 213, 241, 485, 548
CviKI_1 RGCY 7 cut(s) 99, 105, 181, 213, 241, 485, 548
CviQI GTAC 1 cut(s) 35
DdeI CTNAG 3 cut(s) 74, 327, 738
DpnI GATC 3 cut(s) 218, 294, 512
DpnII GATC 3 cut(s) 216, 292, 510
Eco130I CCWWGG 1 cut(s) 418
Eco47III AGCGCT 1 cut(s) 433
EcoRI GAATTC 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 3 cut(s) 198, 400, 422
FatI CATG 3 cut(s) 194, 396, 418
FauNDI CATATG 1 cut(s) 274
FbaI TGATCA 1 cut(s) 216
FblI GTMKAC 2 cut(s) 111, 676
FokI GGATG 1 cut(s) 239
GlaI GCGC 1 cut(s) 433
HaeII RGCGCY 1 cut(s) 435
HapII CCGG 2 cut(s) 63, 393
HhaI GCGC 1 cut(s) 434
Hin1II CATG 3 cut(s) 198, 400, 422
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 3 cut(s) 78, 91, 282
HpaII CCGG 2 cut(s) 63, 393
Hpy166II GTNNAC 5 cut(s) 112, 250, 266, 415, 677
Hpy188I TCNGA 2 cut(s) 169, 741
Hpy188III TCNNGA 2 cut(s) 329, 563
Hpy8I GTNNAC 5 cut(s) 112, 250, 266, 415, 677
HpyAV CCTTC 1 cut(s) 3
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4V TGCA 4 cut(s) 407, 415, 479, 635
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 641, 712
HpyF3I CTNAG 3 cut(s) 74, 327, 738
Hsp92II CATG 3 cut(s) 198, 400, 422
HspAI GCGC 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 3 cut(s) 216, 292, 510
LweI GCATC 1 cut(s) 394
MaeIII GTNAC 1 cut(s) 517
MalI GATC 3 cut(s) 218, 294, 512
MboI GATC 3 cut(s) 216, 292, 510
MboII GAAGA 2 cut(s) 368, 754
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 2 cut(s) 417, 562
MluCI AATT 3 cut(s) 309, 346, 462
MlyI GAGTC 1 cut(s) 291
MmeI TCCRAC 1 cut(s) 259
MnlI CCTC 7 cut(s) 314, 530, 630, 717, 726, 747, 760
MseI TTAA 1 cut(s) 569
MspI CCGG 2 cut(s) 63, 393
MspR9I CCNGG 3 cut(s) 64, 393, 459
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 1 cut(s) 459
MwoI GCNNNNNNNGC 3 cut(s) 38, 641, 712
NciI CCSGG 2 cut(s) 64, 393
NcoI CCATGG 1 cut(s) 418
NdeI CATATG 1 cut(s) 274
NdeII GATC 3 cut(s) 216, 292, 510
NlaIII CATG 3 cut(s) 198, 400, 422
NmuCI GTSAC 1 cut(s) 517
NspI RCATGY 2 cut(s) 198, 400
PaeI GCATGC 1 cut(s) 400
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 2 cut(s) 78, 91
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
PsiI TTATAA 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 457
PspGI CCWGG 1 cut(s) 457
PsrI GAACNNNNNNTAC 2 cut(s) 324, 356
PsuI RGATCY 1 cut(s) 292
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
SaqAI TTAA 1 cut(s) 569
Sau3AI GATC 3 cut(s) 216, 292, 510
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 1 cut(s) 291
ScrFI CCNGG 3 cut(s) 64, 393, 459
SduI GDGCHC 2 cut(s) 417, 562
SetI ASST 4 cut(s) 183, 243, 622, 771
SfaNI GCATC 1 cut(s) 394
SfcI CTRYAG 1 cut(s) 652
SphI GCATGC 1 cut(s) 400
Sse9I AATT 3 cut(s) 309, 346, 462
SspI AATATT 1 cut(s) 135
StyD4I CCNGG 3 cut(s) 62, 391, 457
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 1 cut(s) 157
TasI AATT 3 cut(s) 309, 346, 462
TatI WGTACW 1 cut(s) 34
TfiI GAWTC 2 cut(s) 78, 91
Tru1I TTAA 1 cut(s) 569
Tru9I TTAA 1 cut(s) 569
TseFI GTSAC 1 cut(s) 517
Tsp45I GTSAC 1 cut(s) 517
TspDTI ATGAA 2 cut(s) 83, 540
VneI GTGCAC 1 cut(s) 413
XapI RAATTY 1 cut(s) 462
XceI RCATGY 2 cut(s) 198, 400
XmiI GTMKAC 2 cut(s) 111, 676
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.