RchiOBHm_Chr4g0421311

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002948
Physical Location & Seq
Reverse (-)
46936373 .. 46941399
5027 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39097

Sequence Viewer

Length: 1071 bp
ATGGAGAGAAGGAGATGGTTGCTATTGCTGGCAGTACTTGCGTGCCTGTTTGGTTGTTCTGCCGGGAGAGAACTGAGATTCAAGCACAAGATTCATAACCATAGCCCCGTCTACAATCACACTCTTGCTACAATATTGGTTCGGTATGCTTCTACGGTGTATTTGTCAGATTTGACAGAGCTGTTTTCTTGGACATGCGACGAATGTAATGGCTTGATCAAGGATTTTCAAATGATAGAGCTGATTGTTGACATCCAGCATTGCTTACAGGCATATGTTGGAGTCGCAACAGATCCTAATGCCATTATAATTGCCTTTAGAGGAACTCAGGAACACAGCATACAGAATTGGATTGAAGACTTATTTTGGAAGCAACTTGATATAGATTACCCCGGCATGCCTGATGCAATGGTGCACCATGGGTTTTACAGCGCTTACCACAACACCACCATACGCCCTGGAATTCTAAATGCTGTTGCAAGAGCGAAAGAGTTTTACGGAGATATTGGGATCATAGTGACAGGGCATTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATTTAAGGGTCAATCAAAAAGAGAACAATGTTCAAGTTATGACATTTGGACAACCTCGTATCGGTAATGCAATCTTTGCTACTTACTATAGCAAACTTGTGCCAAAAAGTATACGAATAACAAATGGAAATGATGTTGTGCCTCATTTGCCTCCATACTATACTCACTTTCCTCAGAAGACATACCACCACTTCCCAAGAGAGGTGTGGCTTTATAACATTGGATTGGGAAGTCTTGTTTATAAGGTTGAGAAAATATGTGATGCCTCTGGCGAGGACCCAACATGCAGCAGGTCAGTGAAAGGGAATAGCATTTCAGACCATTTAGTTTATTTTGGTGTCGATTTAATGGCCAAGACATGGAGACCGTGCAAAATTGTGATGGGTCCTGGTTTACTGGAGCATGGAAGAACAGATCTGGAAGGAAATTTTGTACTGTCCAGAGATCTTGCAACTCCTGTTCTCAAATTGAAAACACGGCCAGATGCCCGCGGAAAGCCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

356

Amino Acids

40.23

Weight (kDa)

8.3

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 103 - 241 4e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 308, 780, 807
Acc36I ACCTGC 1 cut(s) 846
AccB7I CCANNNNNTGG 1 cut(s) 924
AccI GTMKAC 2 cut(s) 111, 676
AccII CGCG 1 cut(s) 1056
AciI CCGC 2 cut(s) 1054, 1056
AclWI GGATC 2 cut(s) 287, 518
AcoI YGGCCR 2 cut(s) 915, 1043
AcsI RAATTY 2 cut(s) 462, 991
AfaI GTAC 2 cut(s) 36, 999
AfeI AGCGCT 1 cut(s) 433
AfiI CCNNNNNNNGG 3 cut(s) 626, 766, 924
AgsI TTSAA 6 cut(s) 82, 230, 356, 531, 599, 1036
AjnI CCWGG 2 cut(s) 457, 952
AluBI AGCT 2 cut(s) 181, 241
AluI AGCT 2 cut(s) 181, 241
Alw21I GWGCWC 2 cut(s) 417, 562
Alw26I GTCTC 1 cut(s) 922
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 2 cut(s) 287, 518
Aor51HI AGCGCT 1 cut(s) 433
AoxI GGCC 2 cut(s) 915, 1043
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 1 cut(s) 852
ApoI RAATTY 2 cut(s) 462, 991
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 2 cut(s) 841, 950
AsuC2I CCSGG 2 cut(s) 64, 393
AvaII GGWCC 2 cut(s) 841, 950
BaeGI GKGCMC 1 cut(s) 417
BalI TGGCCA 1 cut(s) 917
BbsI GAAGAC 2 cut(s) 363, 749
Bbv12I GWGCWC 2 cut(s) 417, 562
BbvI GCAGC 1 cut(s) 864
BccI CCATC 2 cut(s) 9, 940
BceAI ACGGC 1 cut(s) 1058
BciT130I CCWGG 2 cut(s) 459, 954
BclI TGATCA 1 cut(s) 216
BcnI CCSGG 2 cut(s) 64, 393
BcoDI GTCTC 1 cut(s) 922
BfmI CTRYAG 2 cut(s) 652, 1067
BfoI RGCGCY 1 cut(s) 435
BfuAI ACCTGC 1 cut(s) 846
BglII AGATCT 2 cut(s) 979, 1009
BisI GCNGC 1 cut(s) 853
BlsI GCNGC 1 cut(s) 854
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 4 cut(s) 64, 393, 459, 954
Bme18I GGWCC 2 cut(s) 841, 950
BmgT120I GGNCC 2 cut(s) 841, 950
BmiI GGNNCC 2 cut(s) 843, 951
BmrFI CCNGG 4 cut(s) 64, 393, 459, 954
BmsI GCATC 3 cut(s) 394, 817, 1039
BpiI GAAGAC 2 cut(s) 363, 749
BpmI CTGGAG 1 cut(s) 983
BpuMI CCSGG 2 cut(s) 64, 393
BsaI GGTCTC 1 cut(s) 922
BsaJI CCNNGG 4 cut(s) 391, 418, 457, 1054
Bsc4I CCNNNNNNNGG 3 cut(s) 626, 766, 924
Bse1I ACTGG 1 cut(s) 966
Bse3DI GCAATG 3 cut(s) 259, 414, 549
BseBI CCWGG 2 cut(s) 459, 954
BseDI CCNNGG 4 cut(s) 391, 418, 457, 1054
BseGI GGATG 1 cut(s) 252
BseLI CCNNNNNNNGG 3 cut(s) 626, 766, 924
BseMI GCAATG 3 cut(s) 259, 414, 549
BseMII CTCAG 3 cut(s) 65, 341, 752
BseNI ACTGG 1 cut(s) 966
BseSI GKGCMC 1 cut(s) 417
BseXI GCAGC 1 cut(s) 864
Bsh1236I CGCG 1 cut(s) 1056
BshFI GGCC 2 cut(s) 917, 1045
BsiHKAI GWGCWC 2 cut(s) 417, 562
BsiSI CCGG 2 cut(s) 63, 393
BslI CCNNNNNNNGG 3 cut(s) 626, 766, 924
BsmAI GTCTC 1 cut(s) 922
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 2 cut(s) 917, 1045
Bso31I GGTCTC 1 cut(s) 922
Bsp1286I GDGCHC 2 cut(s) 417, 562
Bsp143I GATC 5 cut(s) 216, 292, 510, 979, 1009
Bsp19I CCATGG 1 cut(s) 418
BspACI CCGC 2 cut(s) 1054, 1056
BspANI GGCC 2 cut(s) 917, 1045
BspCNI CTCAG 3 cut(s) 66, 340, 751
BspFNI CGCG 1 cut(s) 1056
BspLI GGNNCC 2 cut(s) 843, 951
BspMI ACCTGC 1 cut(s) 846
BspPI GGATC 2 cut(s) 287, 518
BspTNI GGTCTC 1 cut(s) 922
BsrDI GCAATG 3 cut(s) 259, 414, 549
BsrI ACTGG 1 cut(s) 966
BssECI CCNNGG 4 cut(s) 391, 418, 457, 1054
BssMI GATC 5 cut(s) 216, 292, 510, 979, 1009
BssNAI GTATAC 1 cut(s) 677
BssT1I CCWWGG 1 cut(s) 418
Bst1107I GTATAC 1 cut(s) 677
Bst2UI CCWGG 2 cut(s) 459, 954
Bst4CI ACNGT 3 cut(s) 157, 933, 1002
BstAPI GCANNNNNTGC 2 cut(s) 38, 641
BstC8I GCNNGC 4 cut(s) 30, 43, 398, 1054
BstDEI CTNAG 3 cut(s) 74, 327, 738
BstDSI CCRYGG 2 cut(s) 418, 1054
BstF5I GGATG 1 cut(s) 252
BstFNI CGCG 1 cut(s) 1056
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 5 cut(s) 219, 295, 513, 982, 1012
BstMAI GTCTC 1 cut(s) 922
BstMBI GATC 5 cut(s) 216, 292, 510, 979, 1009
BstMWI GCNNNNNNNGC 3 cut(s) 38, 641, 712
BstNI CCWGG 2 cut(s) 459, 954
BstNSI RCATGY 3 cut(s) 198, 400, 852
BstSCI CCNGG 4 cut(s) 62, 391, 457, 952
BstSFI CTRYAG 2 cut(s) 652, 1067
BstSLI GKGCMC 1 cut(s) 417
BstUI CGCG 1 cut(s) 1056
BstV1I GCAGC 1 cut(s) 864
BstV2I GAAGAC 2 cut(s) 363, 749
BstX2I RGATCY 3 cut(s) 292, 979, 1009
BstYI RGATCY 3 cut(s) 292, 979, 1009
BstZ17I GTATAC 1 cut(s) 677
BsuRI GGCC 2 cut(s) 917, 1045
BtgI CCRYGG 2 cut(s) 418, 1054
BtsCI GGATG 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 867
BveI ACCTGC 1 cut(s) 846
Cac8I GCNNGC 4 cut(s) 30, 43, 398, 1054
CfoI GCGC 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 841, 950
Cfr42I CCGCGG 1 cut(s) 1057
Csp6I GTAC 2 cut(s) 35, 998
CviAII CATG 6 cut(s) 195, 397, 419, 849, 924, 968
CviJI RGCY 9 cut(s) 105, 181, 213, 241, 548, 775, 917, 1045, 1063
CviKI_1 RGCY 9 cut(s) 105, 181, 213, 241, 548, 775, 917, 1045, 1063
CviQI GTAC 2 cut(s) 35, 998
DdeI CTNAG 3 cut(s) 74, 327, 738
DpnI GATC 5 cut(s) 218, 294, 512, 981, 1011
DpnII GATC 5 cut(s) 216, 292, 510, 979, 1009
EaeI YGGCCR 2 cut(s) 915, 1043
Eco130I CCWWGG 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 922
Eco47I GGWCC 2 cut(s) 841, 950
Eco47III AGCGCT 1 cut(s) 433
EcoO109I RGGNCCY 2 cut(s) 841, 950
EcoRI GAATTC 1 cut(s) 462
EcoRII CCWGG 2 cut(s) 457, 952
EcoT14I CCWWGG 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 6 cut(s) 198, 400, 422, 852, 927, 971
FatI CATG 6 cut(s) 194, 396, 418, 848, 923, 967
FauI CCCGC 1 cut(s) 1061
FauNDI CATATG 1 cut(s) 274
FbaI TGATCA 1 cut(s) 216
FblI GTMKAC 2 cut(s) 111, 676
Fnu4HI GCNGC 1 cut(s) 853
FokI GGATG 1 cut(s) 239
Fsp4HI GCNGC 1 cut(s) 853
GlaI GCGC 1 cut(s) 433
GluI GCNGC 1 cut(s) 853
GsuI CTGGAG 1 cut(s) 983
HaeII RGCGCY 1 cut(s) 435
HaeIII GGCC 2 cut(s) 917, 1045
HapII CCGG 2 cut(s) 63, 393
HhaI GCGC 1 cut(s) 434
Hin1II CATG 6 cut(s) 198, 400, 422, 852, 927, 971
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HinfI GANTC 3 cut(s) 78, 91, 282
HpaII CCGG 2 cut(s) 63, 393
Hpy166II GTNNAC 5 cut(s) 112, 250, 415, 677, 959
Hpy188I TCNGA 3 cut(s) 169, 741, 883
Hpy188III TCNNGA 4 cut(s) 329, 563, 983, 1005
Hpy8I GTNNAC 5 cut(s) 112, 250, 415, 677, 959
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 2 cut(s) 3, 980
HpyCH4III ACNGT 3 cut(s) 157, 933, 1002
HpyCH4V TGCA 7 cut(s) 407, 415, 479, 635, 852, 936, 1016
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 641, 712
HpyF3I CTNAG 3 cut(s) 74, 327, 738
Hsp92II CATG 6 cut(s) 198, 400, 422, 852, 927, 971
HspAI GCGC 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 216
KspI CCGCGG 1 cut(s) 1057
Kzo9I GATC 5 cut(s) 216, 292, 510, 979, 1009
LmnI GCTCC 1 cut(s) 964
Lsp1109I GCAGC 1 cut(s) 864
LweI GCATC 3 cut(s) 394, 817, 1039
MaeIII GTNAC 1 cut(s) 517
MalI GATC 5 cut(s) 218, 294, 512, 981, 1011
MboI GATC 5 cut(s) 216, 292, 510, 979, 1009
MboII GAAGA 3 cut(s) 368, 754, 984
MflI RGATCY 3 cut(s) 292, 979, 1009
MhlI GDGCHC 2 cut(s) 417, 562
MlsI TGGCCA 1 cut(s) 917
MluCI AATT 6 cut(s) 309, 346, 462, 939, 991, 1031
MluNI TGGCCA 1 cut(s) 917
MlyI GAGTC 1 cut(s) 291
MmeI TCCRAC 1 cut(s) 259
MnlI CCTC 9 cut(s) 314, 530, 630, 717, 726, 747, 760, 832, 841
Mox20I TGGCCA 1 cut(s) 917
MscI TGGCCA 1 cut(s) 917
MseI TTAA 2 cut(s) 569, 911
Msp20I TGGCCA 1 cut(s) 917
MspA1I CMGCKG 1 cut(s) 1056
MspI CCGG 2 cut(s) 63, 393
MspR9I CCNGG 4 cut(s) 64, 393, 459, 954
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 2 cut(s) 459, 954
MvnI CGCG 1 cut(s) 1056
MwoI GCNNNNNNNGC 3 cut(s) 38, 641, 712
NciI CCSGG 2 cut(s) 64, 393
NcoI CCATGG 1 cut(s) 418
NdeI CATATG 1 cut(s) 274
NdeII GATC 5 cut(s) 216, 292, 510, 979, 1009
NlaIII CATG 6 cut(s) 198, 400, 422, 852, 927, 971
NlaIV GGNNCC 2 cut(s) 843, 951
NmuCI GTSAC 1 cut(s) 517
NspI RCATGY 3 cut(s) 198, 400, 852
PaeI GCATGC 1 cut(s) 400
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 2 cut(s) 78, 91
PflMI CCANNNNNTGG 1 cut(s) 924
PkrI GCNGC 1 cut(s) 854
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
PpuMI RGGWCCY 2 cut(s) 841, 950
PsiI TTATAA 3 cut(s) 308, 780, 807
Psp5II RGGWCCY 2 cut(s) 841, 950
Psp6I CCWGG 2 cut(s) 457, 952
PspGI CCWGG 2 cut(s) 457, 952
PspN4I GGNNCC 2 cut(s) 843, 951
PspPI GGNCC 2 cut(s) 841, 950
PspPPI RGGWCCY 2 cut(s) 841, 950
PsrI GAACNNNNNNTAC 2 cut(s) 324, 356
PsuI RGATCY 3 cut(s) 292, 979, 1009
RsaI GTAC 2 cut(s) 36, 999
RsaNI GTAC 2 cut(s) 35, 998
SacII CCGCGG 1 cut(s) 1057
SaqAI TTAA 2 cut(s) 569, 911
SatI GCNGC 1 cut(s) 853
Sau3AI GATC 5 cut(s) 216, 292, 510, 979, 1009
Sau96I GGNCC 2 cut(s) 841, 950
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 1 cut(s) 291
ScrFI CCNGG 4 cut(s) 64, 393, 459, 954
SduI GDGCHC 2 cut(s) 417, 562
SetI ASST 6 cut(s) 183, 243, 622, 771, 813, 860
SfaNI GCATC 3 cut(s) 394, 817, 1039
SfcI CTRYAG 2 cut(s) 652, 1067
Sfr303I CCGCGG 1 cut(s) 1057
SgrBI CCGCGG 1 cut(s) 1057
SinI GGWCC 2 cut(s) 841, 950
SphI GCATGC 1 cut(s) 400
Sse9I AATT 6 cut(s) 309, 346, 462, 939, 991, 1031
SsiI CCGC 2 cut(s) 1054, 1056
SspI AATATT 1 cut(s) 135
StyD4I CCNGG 4 cut(s) 62, 391, 457, 952
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 3 cut(s) 157, 933, 1002
TaqI TCGA 1 cut(s) 906
TasI AATT 6 cut(s) 309, 346, 462, 939, 991, 1031
TatI WGTACW 2 cut(s) 34, 997
TfiI GAWTC 2 cut(s) 78, 91
Tru1I TTAA 2 cut(s) 569, 911
Tru9I TTAA 2 cut(s) 569, 911
TscAI CASTG 1 cut(s) 867
TseFI GTSAC 1 cut(s) 517
TseI GCWGC 1 cut(s) 852
Tsp45I GTSAC 1 cut(s) 517
TspDTI ATGAA 2 cut(s) 83, 540
TspGWI ACGGA 1 cut(s) 513
TspRI CASTG 1 cut(s) 867
Van91I CCANNNNNTGG 1 cut(s) 924
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 2 cut(s) 841, 950
XapI RAATTY 2 cut(s) 462, 991
XceI RCATGY 3 cut(s) 198, 400, 852
XcmI CCANNNNNNNNNTGG 1 cut(s) 768
XmiI GTMKAC 2 cut(s) 111, 676
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.