AT5G20100

at1g15400

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004606
Physical Location & Seq
Forward (+)
6789935 .. 6790841
907 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20100.1

Sequence Viewer

Length: 342 bp
ATGGCGTTGCAGAGATCAACAGCGTCGTTTAGGAGACAAGGATCGTCAGGACTCATCTGGAACGACAGGTTCTTGAGCGGAGAAATTCGCAACGACGAAAGACAAGAAGACCGGTGCAACGATCATCGTGACGGATCAATGGCTGCTACGACTGCAACAGTGAAACGTAGCGCATCAGACGGAGGATGTGGCCATGGTGGAAGATTGGGGGAGCTATCTCCAGCGTTGGATCCACCTTCACCTAAAATATCTGCCGTTGGTTGTGGCTTTTGTTCTTTGTTTTCGTCTAATGGGAAAAGAAGAAGCAGAGGCAGGAGAAGATCAAGCGGTGGAAGTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

113

Amino Acids

12.06

Weight (kDa)

10.63

Isoelectric Point (pI)

82.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 2 - 101 8.6e-25 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 78
AciI CCGC 2 cut(s) 78, 327
AclWI GGATC 4 cut(s) 49, 142, 224, 237
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 1 cut(s) 84
AfaI GTAC 1 cut(s) 337
AgeI ACCGGT 1 cut(s) 111
AluBI AGCT 1 cut(s) 214
AluI AGCT 1 cut(s) 214
Alw26I GTCTC 1 cut(s) 28
AlwI GGATC 4 cut(s) 49, 142, 224, 237
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 1 cut(s) 143
ApoI RAATTY 1 cut(s) 84
AsiGI ACCGGT 1 cut(s) 111
AspLEI GCGC 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 231
BalI TGGCCA 1 cut(s) 192
BamHI GGATCC 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 114
BbvI GCAGC 1 cut(s) 130
BceAI ACGGC 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 28
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
BmcAI AGTACT 1 cut(s) 337
BmiI GGNNCC 1 cut(s) 231
BmsI GCATC 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 94
BsaJI CCNNGG 1 cut(s) 193
BsaWI WCCGGW 1 cut(s) 111
Bse118I RCCGGY 1 cut(s) 111
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 1 cut(s) 191
BseXI GCAGC 1 cut(s) 130
BshFI GGCC 1 cut(s) 192
BshTI ACCGGT 1 cut(s) 111
BsiSI CCGG 1 cut(s) 112
BsmAI GTCTC 1 cut(s) 28
BsnI GGCC 1 cut(s) 192
Bsp143I GATC 6 cut(s) 14, 41, 121, 134, 229, 320
Bsp19I CCATGG 1 cut(s) 193
BspACI CCGC 2 cut(s) 78, 327
BspANI GGCC 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 231
BspPI GGATC 4 cut(s) 49, 142, 224, 237
BsrBI CCGCTC 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 111
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 6 cut(s) 14, 41, 121, 134, 229, 320
BssT1I CCWWGG 1 cut(s) 193
Bst4CI ACNGT 1 cut(s) 160
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 1 cut(s) 191
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 6 cut(s) 17, 44, 124, 137, 232, 323
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 6 cut(s) 14, 41, 121, 134, 229, 320
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstV1I GCAGC 1 cut(s) 130
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BsuRI GGCC 1 cut(s) 192
BtgI CCRYGG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 191
BtsIMutI CAGTG 1 cut(s) 165
CfoI GCGC 1 cut(s) 173
Cfr10I RCCGGY 1 cut(s) 111
CseI GACGC 1 cut(s) 12
Csp6I GTAC 1 cut(s) 336
CspAI ACCGGT 1 cut(s) 111
CviAII CATG 1 cut(s) 194
CviJI RGCY 4 cut(s) 143, 192, 214, 267
CviKI_1 RGCY 4 cut(s) 143, 192, 214, 267
CviQI GTAC 1 cut(s) 336
DpnI GATC 6 cut(s) 16, 43, 123, 136, 231, 322
DpnII GATC 6 cut(s) 14, 41, 121, 134, 229, 320
EaeI YGGCCR 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 1 cut(s) 197
FaiI YATR 1 cut(s) 195
FatI CATG 1 cut(s) 193
Fnu4HI GCNGC 1 cut(s) 144
FokI GGATG 1 cut(s) 198
Fsp4HI GCNGC 1 cut(s) 144
GlaI GCGC 1 cut(s) 172
GluI GCNGC 1 cut(s) 144
GsuI CTGGAG 1 cut(s) 204
HaeIII GGCC 1 cut(s) 192
HapII CCGG 1 cut(s) 112
HgaI GACGC 1 cut(s) 12
HhaI GCGC 1 cut(s) 173
Hin1II CATG 1 cut(s) 197
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HinfI GANTC 1 cut(s) 51
HpaII CCGG 1 cut(s) 112
HphI GGTGA 1 cut(s) 231
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 4 cut(s) 48, 58, 73, 128
Hpy99I CGWCG 2 cut(s) 28, 98
HpyAV CCTTC 1 cut(s) 246
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 3 cut(s) 10, 117, 155
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpySE526I ACGT 1 cut(s) 166
Hsp92II CATG 1 cut(s) 197
HspAI GCGC 1 cut(s) 171
Kzo9I GATC 6 cut(s) 14, 41, 121, 134, 229, 320
LmnI GCTCC 1 cut(s) 211
LpnPI CCDG 6 cut(s) 33, 43, 52, 125, 234, 298
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 1 cut(s) 182
MaeII ACGT 1 cut(s) 166
MaeIII GTNAC 1 cut(s) 128
MalI GATC 6 cut(s) 16, 43, 123, 136, 231, 322
MbiI CCGCTC 1 cut(s) 78
MboI GATC 6 cut(s) 14, 41, 121, 134, 229, 320
MboII GAAGA 4 cut(s) 119, 213, 312, 330
MflI RGATCY 1 cut(s) 229
MlsI TGGCCA 1 cut(s) 192
MluCI AATT 1 cut(s) 84
MluNI TGGCCA 1 cut(s) 192
MlyI GAGTC 1 cut(s) 45
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 2 cut(s) 176, 302
Mox20I TGGCCA 1 cut(s) 192
MscI TGGCCA 1 cut(s) 192
MseI TTAA 1 cut(s) 340
Msp20I TGGCCA 1 cut(s) 192
MspI CCGG 1 cut(s) 112
MwoI GCNNNNNNNGC 1 cut(s) 152
NcoI CCATGG 1 cut(s) 193
NdeII GATC 6 cut(s) 14, 41, 121, 134, 229, 320
NlaIII CATG 1 cut(s) 197
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 128
PinAI ACCGGT 1 cut(s) 111
PkrI GCNGC 1 cut(s) 145
PleI GAGTC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 231
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
SaqAI TTAA 1 cut(s) 340
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 6 cut(s) 14, 41, 121, 134, 229, 320
ScaI AGTACT 1 cut(s) 337
SchI GAGTC 1 cut(s) 45
SetI ASST 5 cut(s) 71, 169, 216, 238, 244
SfaNI GCATC 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 1 cut(s) 84
SsiI CCGC 2 cut(s) 78, 327
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 1 cut(s) 160
TaiI ACGT 1 cut(s) 169
TasI AATT 1 cut(s) 84
TatI WGTACW 1 cut(s) 335
Tru1I TTAA 1 cut(s) 340
Tru9I TTAA 1 cut(s) 340
TscAI CASTG 1 cut(s) 165
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 1 cut(s) 143
Tsp45I GTSAC 1 cut(s) 128
TspGWI ACGGA 2 cut(s) 147, 195
TspRI CASTG 1 cut(s) 165
XapI RAATTY 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.