RLG00000019533

Domain of unknown function (DUF4666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004606
Physical Location & Seq
Reverse (-)
53232989 .. 53237573
4585 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019533

Sequence Viewer

Length: 822 bp
ATGGCTGGTTTGCAAAGATCTTCAATGTCTTTCAGAAGGCAAGGCTCTTCAGGGTTTGTGTGGGATGACAGGTTCTTGTCCGGAGAGCTAAAGCAACAACCACCAGCACAAGACCAAGAAAGCTCTGAGGAGAATGAGATTGTCATAGTTAAGGATGTTAAGCCACTGAGGCCAATCAAGACTGTACAACGAAGCAGATCCAACGGCGGAGGACGCGGCTACCGCAGCTCTGGGAAGATCTCGCCGGCTATTGAACCACCGTCGCCAAAGGTTTCTGCATGTGGGTTTTGTGGGGGTTTCGGTAAATCCTCCAAGAACAATGCGGTTCGTGGAGCAAAGCCTGGCAAGCACATGACTGCGCTGACGGCGTTCCATCGAACGAACGGGTGGCGGTGCTCCAGATTCGCCAGAATCGACGCTCAACGGCTAGACGATCTCGGCCTCGTTCAATCGGCAAAGGAATCGGAGCGTGACGCCGCGGATGTACTCGTTAGGGCTCTGGAGATTGTTGCAGAGGTTCTGGCAGCTGAGGATCATCTCCGGGAGAACTTTGCCTCGGGAATCTGTCTTGTCAATGATCTCGAGGTAGAGGAGGAGAAACTTCTGGACGGTGTGGTCCTCGAAGGGGAGGACGTAGCGGATAATAGTGATGAAGAGCTGGGGAATTGTGTCGCCGTTGAGCAAGAGCATGATGGCCTTCTTCATGGCTTCAATCTTGGCTTCGATGTCGTTTCCTTCGAGAGCCGGAGACTATCGATCGTAAGTTTGGAAGTCAATATGCATCCAATCAAGAAACCTGATCTCACAACTATTTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

274

Amino Acids

29.96

Weight (kDa)

5.39

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 1 - 107 1.3e-33 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 614
AccII CGCG 2 cut(s) 216, 479
AccIII TCCGGA 1 cut(s) 80
AciI CCGC 8 cut(s) 207, 216, 223, 323, 391, 477, 479, 638
AclWI GGATC 2 cut(s) 192, 540
AcuI CTGAAG 1 cut(s) 33
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 2 cut(s) 186, 486
AfiI CCNNNNNNNGG 1 cut(s) 625
AgsI TTSAA 4 cut(s) 24, 254, 449, 712
AjnI CCWGG 1 cut(s) 340
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
AluBI AGCT 5 cut(s) 88, 123, 228, 527, 658
AluI AGCT 5 cut(s) 88, 123, 228, 527, 658
Alw21I GWGCWC 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 742
AlwI GGATC 2 cut(s) 192, 540
Ama87I CYCGRG 2 cut(s) 556, 581
Aor13HI TCCGGA 1 cut(s) 80
AoxI GGCC 3 cut(s) 170, 439, 694
ApeKI GCWGC 2 cut(s) 225, 524
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 1 cut(s) 616
AsuC2I CCSGG 1 cut(s) 542
AvaI CYCGRG 2 cut(s) 556, 581
AvaII GGWCC 1 cut(s) 616
BanII GRGCYC 1 cut(s) 499
Bbv12I GWGCWC 1 cut(s) 398
BbvCI CCTCAGC 1 cut(s) 528
BbvI GCAGC 2 cut(s) 237, 536
BccI CCATC 2 cut(s) 381, 686
BceAI ACGGC 4 cut(s) 220, 381, 440, 659
BcgI CGANNNNNNTGC 2 cut(s) 444, 478
BciT130I CCWGG 1 cut(s) 342
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 1 cut(s) 742
BfaI CTAG 1 cut(s) 428
BglI GCCNNNNNGGC 1 cut(s) 169
BglII AGATCT 2 cut(s) 17, 237
BisI GCNGC 4 cut(s) 217, 226, 477, 525
BlsI GCNGC 4 cut(s) 218, 227, 478, 526
Bme1390I CCNGG 2 cut(s) 342, 542
Bme18I GGWCC 1 cut(s) 616
BmeT110I CYCGRG 2 cut(s) 556, 581
BmgT120I GGNCC 1 cut(s) 616
BmrFI CCNGG 2 cut(s) 342, 542
BmsI GCATC 1 cut(s) 790
BpmI CTGGAG 2 cut(s) 382, 521
Bpu10I CCTNAGC 1 cut(s) 528
BpuMI CCSGG 1 cut(s) 542
Bsa29I ATCGAT 1 cut(s) 755
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 2 cut(s) 477, 555
BsaWI WCCGGW 1 cut(s) 80
Bsc4I CCNNNNNNNGG 1 cut(s) 625
Bse118I RCCGGY 1 cut(s) 244
BseAI TCCGGA 1 cut(s) 80
BseBI CCWGG 1 cut(s) 342
BseCI ATCGAT 1 cut(s) 755
BseDI CCNNGG 2 cut(s) 477, 555
BseGI GGATG 4 cut(s) 70, 160, 487, 781
BseLI CCNNNNNNNGG 1 cut(s) 625
BseMII CTCAG 3 cut(s) 117, 158, 519
BseRI GAGGAG 3 cut(s) 143, 605, 608
BseXI GCAGC 2 cut(s) 237, 536
BseYI CCCAGC 1 cut(s) 658
Bsh1236I CGCG 2 cut(s) 216, 479
Bsh1285I CGRYCG 1 cut(s) 759
BshFI GGCC 3 cut(s) 172, 441, 696
BshVI ATCGAT 1 cut(s) 755
BsiEI CGRYCG 1 cut(s) 759
BsiHKAI GWGCWC 1 cut(s) 398
BsiHKCI CYCGRG 2 cut(s) 556, 581
BsiSI CCGG 4 cut(s) 81, 245, 541, 745
BslI CCNNNNNNNGG 1 cut(s) 625
BsmAI GTCTC 1 cut(s) 742
BsnI GGCC 3 cut(s) 172, 441, 696
BsoBI CYCGRG 2 cut(s) 556, 581
Bsp1286I GDGCHC 2 cut(s) 398, 499
Bsp13I TCCGGA 1 cut(s) 80
Bsp1407I TGTACA 1 cut(s) 184
Bsp143I GATC 8 cut(s) 17, 197, 237, 433, 532, 577, 756, 799
BspACI CCGC 8 cut(s) 207, 216, 223, 323, 391, 477, 479, 638
BspANI GGCC 3 cut(s) 172, 441, 696
BspCNI CTCAG 3 cut(s) 118, 159, 520
BspDI ATCGAT 1 cut(s) 755
BspEI TCCGGA 1 cut(s) 80
BspFNI CGCG 2 cut(s) 216, 479
BspPI GGATC 2 cut(s) 192, 540
BspQI GCTCTTC 2 cut(s) 52, 648
BsrFI RCCGGY 1 cut(s) 244
BsrGI TGTACA 1 cut(s) 184
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 2 cut(s) 477, 555
BssMI GATC 8 cut(s) 17, 197, 237, 433, 532, 577, 756, 799
BssNI GRCGYC 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 3 cut(s) 184, 261, 611
Bst6I CTCTTC 2 cut(s) 52, 648
BstACI GRCGYC 1 cut(s) 474
BstAUI TGTACA 1 cut(s) 184
BstC8I GCNNGC 2 cut(s) 246, 347
BstDEI CTNAG 3 cut(s) 126, 167, 528
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 4 cut(s) 70, 160, 487, 781
BstFNI CGCG 2 cut(s) 216, 479
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 8 cut(s) 20, 200, 240, 436, 535, 580, 759, 802
BstMAI GTCTC 1 cut(s) 742
BstMBI GATC 8 cut(s) 17, 197, 237, 433, 532, 577, 756, 799
BstMCI CGRYCG 1 cut(s) 759
BstMWI GCNNNNNNNGC 6 cut(s) 169, 213, 222, 225, 346, 365
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 282
BstSCI CCNGG 2 cut(s) 340, 540
BstUI CGCG 2 cut(s) 216, 479
BstV1I GCAGC 2 cut(s) 237, 536
BstX2I RGATCY 3 cut(s) 17, 197, 237
BstYI RGATCY 3 cut(s) 17, 197, 237
Bsu15I ATCGAT 1 cut(s) 755
BsuRI GGCC 3 cut(s) 172, 441, 696
BsuTUI ATCGAT 1 cut(s) 755
BtgI CCRYGG 1 cut(s) 477
BtsCI GGATG 4 cut(s) 70, 160, 487, 781
BtsIMutI CAGTG 1 cut(s) 164
Cac8I GCNNGC 2 cut(s) 246, 347
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 1 cut(s) 616
Cfr42I CCGCGG 1 cut(s) 480
ClaI ATCGAT 1 cut(s) 755
CseI GACGC 3 cut(s) 222, 425, 482
Csp6I GTAC 2 cut(s) 185, 485
CviAII CATG 4 cut(s) 279, 352, 689, 704
CviQI GTAC 2 cut(s) 185, 485
DdeI CTNAG 3 cut(s) 126, 167, 528
DpnI GATC 8 cut(s) 19, 199, 239, 435, 534, 579, 758, 801
DpnII GATC 8 cut(s) 17, 197, 237, 433, 532, 577, 756, 799
DrdI GACNNNNNNGTC 1 cut(s) 614
DseDI GACNNNNNNGTC 1 cut(s) 614
Eam1104I CTCTTC 2 cut(s) 52, 648
EarI CTCTTC 2 cut(s) 52, 648
EciI GGCGGA 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 499
Eco47I GGWCC 1 cut(s) 616
Eco57I CTGAAG 1 cut(s) 33
Eco88I CYCGRG 2 cut(s) 556, 581
EcoRII CCWGG 1 cut(s) 340
EcoT22I ATGCAT 1 cut(s) 783
EcoT38I GRGCYC 1 cut(s) 499
FaeI CATG 4 cut(s) 282, 355, 692, 707
FaiI YATR 6 cut(s) 146, 280, 353, 690, 705, 779
FatI CATG 4 cut(s) 278, 351, 688, 703
Fnu4HI GCNGC 4 cut(s) 217, 226, 477, 525
FokI GGATG 4 cut(s) 77, 167, 494, 768
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 4 cut(s) 217, 226, 477, 525
FspBI CTAG 1 cut(s) 428
GlaI GCGC 1 cut(s) 360
GluI GCNGC 4 cut(s) 217, 226, 477, 525
GsaI CCCAGC 1 cut(s) 662
GsuI CTGGAG 2 cut(s) 382, 521
HaeIII GGCC 3 cut(s) 172, 441, 696
HapII CCGG 4 cut(s) 81, 245, 541, 745
HgaI GACGC 3 cut(s) 222, 425, 482
HhaI GCGC 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 4 cut(s) 282, 355, 692, 707
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HinfI GANTC 4 cut(s) 402, 411, 461, 561
HpaII CCGG 4 cut(s) 81, 245, 541, 745
Hpy188I TCNGA 3 cut(s) 35, 127, 466
Hpy188III TCNNGA 9 cut(s) 81, 178, 399, 500, 558, 581, 605, 739, 790
Hpy99I CGWCG 2 cut(s) 265, 419
HpyAV CCTTC 4 cut(s) 30, 617, 707, 745
HpyCH4III ACNGT 3 cut(s) 184, 261, 611
HpyCH4IV ACGT 1 cut(s) 633
HpyCH4V TGCA 4 cut(s) 13, 278, 512, 781
HpyF10VI GCNNNNNNNGC 6 cut(s) 169, 213, 222, 225, 346, 365
HpyF3I CTNAG 3 cut(s) 126, 167, 528
HpySE526I ACGT 1 cut(s) 633
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 4 cut(s) 282, 355, 692, 707
HspAI GCGC 1 cut(s) 359
Kpn2I TCCGGA 1 cut(s) 80
KroI GCCGGC 1 cut(s) 244
KroNI GCCGGC 1 cut(s) 246
KspI CCGCGG 1 cut(s) 480
Kzo9I GATC 8 cut(s) 17, 197, 237, 433, 532, 577, 756, 799
LguI GCTCTTC 2 cut(s) 52, 648
LmnI GCTCC 3 cut(s) 332, 401, 466
Lsp1109I GCAGC 2 cut(s) 237, 536
LweI GCATC 1 cut(s) 790
MaeI CTAG 1 cut(s) 428
MaeII ACGT 1 cut(s) 633
MaeIII GTNAC 1 cut(s) 470
MalI GATC 8 cut(s) 19, 199, 239, 435, 534, 579, 758, 801
MboI GATC 8 cut(s) 17, 197, 237, 433, 532, 577, 756, 799
MboII GAAGA 5 cut(s) 12, 39, 247, 665, 692
MflI RGATCY 3 cut(s) 17, 197, 237
MhlI GDGCHC 2 cut(s) 398, 499
MluCI AATT 1 cut(s) 664
MmeI TCCRAC 1 cut(s) 225
Mph1103I ATGCAT 1 cut(s) 783
MroI TCCGGA 1 cut(s) 80
MroNI GCCGGC 1 cut(s) 244
MseI TTAA 2 cut(s) 150, 159
MspA1I CMGCKG 2 cut(s) 479, 527
MspI CCGG 4 cut(s) 81, 245, 541, 745
MspR9I CCNGG 2 cut(s) 342, 542
MvaI CCWGG 1 cut(s) 342
MvnI CGCG 2 cut(s) 216, 479
MwoI GCNNNNNNNGC 6 cut(s) 169, 213, 222, 225, 346, 365
NaeI GCCGGC 1 cut(s) 246
NciI CCSGG 1 cut(s) 542
NdeII GATC 8 cut(s) 17, 197, 237, 433, 532, 577, 756, 799
NgoMIV GCCGGC 1 cut(s) 244
NlaIII CATG 4 cut(s) 282, 355, 692, 707
NmeAIII GCCGAG 1 cut(s) 417
NmuCI GTSAC 1 cut(s) 470
NsiI ATGCAT 1 cut(s) 783
NspI RCATGY 1 cut(s) 282
PaeR7I CTCGAG 1 cut(s) 581
PciSI GCTCTTC 2 cut(s) 52, 648
PcsI WCGNNNNNNNCGW 2 cut(s) 411, 735
PdiI GCCGGC 1 cut(s) 246
PfeI GAWTC 4 cut(s) 402, 411, 461, 561
PfoI TCCNGGA 1 cut(s) 540
PkrI GCNGC 4 cut(s) 218, 227, 478, 526
Ple19I CGATCG 1 cut(s) 759
Psp6I CCWGG 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 658
PspGI CCWGG 1 cut(s) 340
PspPI GGNCC 1 cut(s) 616
PsuI RGATCY 3 cut(s) 17, 197, 237
PvuI CGATCG 1 cut(s) 759
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 2 cut(s) 186, 486
RsaNI GTAC 2 cut(s) 185, 485
SacII CCGCGG 1 cut(s) 480
SapI GCTCTTC 2 cut(s) 52, 648
SaqAI TTAA 2 cut(s) 150, 159
SatI GCNGC 4 cut(s) 217, 226, 477, 525
Sau3AI GATC 8 cut(s) 17, 197, 237, 433, 532, 577, 756, 799
Sau96I GGNCC 1 cut(s) 616
ScrFI CCNGG 2 cut(s) 342, 542
SduI GDGCHC 2 cut(s) 398, 499
SfaNI GCATC 1 cut(s) 790
Sfr274I CTCGAG 1 cut(s) 581
Sfr303I CCGCGG 1 cut(s) 480
SgrBI CCGCGG 1 cut(s) 480
SinI GGWCC 1 cut(s) 616
SlaI CTCGAG 1 cut(s) 581
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 1 cut(s) 664
SsiI CCGC 8 cut(s) 207, 216, 223, 323, 391, 477, 479, 638
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 2 cut(s) 340, 540
TaaI ACNGT 3 cut(s) 184, 261, 611
TaiI ACGT 1 cut(s) 636
TaqI TCGA 7 cut(s) 376, 414, 582, 621, 723, 738, 755
TasI AATT 1 cut(s) 664
TatI WGTACW 2 cut(s) 184, 484
TauI GCSGC 2 cut(s) 219, 479
TfiI GAWTC 4 cut(s) 402, 411, 461, 561
Tru1I TTAA 2 cut(s) 150, 159
Tru9I TTAA 2 cut(s) 150, 159
TscAI CASTG 1 cut(s) 171
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 2 cut(s) 225, 524
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 2 cut(s) 666, 692
TspRI CASTG 1 cut(s) 171
VpaK11BI GGWCC 1 cut(s) 616
XceI RCATGY 1 cut(s) 282
XhoI CTCGAG 1 cut(s) 581
XspI CTAG 1 cut(s) 428
Zsp2I ATGCAT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.