RchiOBHm_Chr2g0136461

Domain of unknown function (DUF4666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004606
Physical Location & Seq
Reverse (-)
53848553 .. 53848921
369 bp
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UTR
Exon/CDS
Intron
PRQ50725

Sequence Viewer

Length: 369 bp
ATGGCTGGTTTGCAAAGATCTTCAATGTCTTTCAGAAGGCAAGGCTCTTCAGGGTTTGTGTGGGATGACAGGTTCTTGTCCGGAGAGCTAAAGCAACAACAACCAGCACAAGACCAAGAAAGCTCTGAGGAGAATGAGATTGTCATAGTTAAGGATGTTAAGCCACTGAGGCCAATCAAGACTGTACAACGAAGCAGATCCAACGGCGGAGGATGCGGCTACCGGAGCTCTGGGAAGATCTCGCCGGCTATTGAACCACCGTCGCCAAAGGTCTCTGCATGTGGGTTTTGTGGGGGTTTCGGTAAATCCTCCAAGAACAATGCGGTTCGGGGAGCAAAGCCTGGCAAGCGTAGCCGATCACGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

122

Amino Acids

13.24

Weight (kDa)

10.29

Isoelectric Point (pI)

91.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 1 - 107 2.1e-34 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 80
AciI CCGC 3 cut(s) 207, 216, 323
AclWI GGATC 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 33
AfaI GTAC 1 cut(s) 186
AgsI TTSAA 2 cut(s) 24, 254
AjnI CCWGG 1 cut(s) 340
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
AluBI AGCT 3 cut(s) 88, 123, 228
AluI AGCT 3 cut(s) 88, 123, 228
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 1 cut(s) 277
AlwI GGATC 1 cut(s) 192
Aor13HI TCCGGA 1 cut(s) 80
AoxI GGCC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 230
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 1 cut(s) 342
BcoDI GTCTC 1 cut(s) 277
BglI GCCNNNNNGGC 1 cut(s) 169
BglII AGATCT 2 cut(s) 17, 237
BisI GCNGC 1 cut(s) 217
BlsI GCNGC 1 cut(s) 218
Bme1390I CCNGG 1 cut(s) 342
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 277
BsaWI WCCGGW 2 cut(s) 80, 222
Bse118I RCCGGY 1 cut(s) 244
BseAI TCCGGA 1 cut(s) 80
BseBI CCWGG 1 cut(s) 342
BseGI GGATG 3 cut(s) 70, 160, 218
BseMII CTCAG 2 cut(s) 117, 158
BseRI GAGGAG 1 cut(s) 143
BshFI GGCC 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 230
BsiSI CCGG 3 cut(s) 81, 223, 245
BsmAI GTCTC 1 cut(s) 277
BsnI GGCC 1 cut(s) 172
Bso31I GGTCTC 1 cut(s) 277
Bsp1286I GDGCHC 1 cut(s) 230
Bsp13I TCCGGA 1 cut(s) 80
Bsp1407I TGTACA 1 cut(s) 184
Bsp143I GATC 4 cut(s) 17, 197, 237, 356
BspACI CCGC 3 cut(s) 207, 216, 323
BspANI GGCC 1 cut(s) 172
BspCNI CTCAG 2 cut(s) 118, 159
BspEI TCCGGA 1 cut(s) 80
BspPI GGATC 1 cut(s) 192
BspQI GCTCTTC 1 cut(s) 52
BspTNI GGTCTC 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 244
BsrGI TGTACA 1 cut(s) 184
BssAI RCCGGY 1 cut(s) 244
BssMI GATC 4 cut(s) 17, 197, 237, 356
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 184, 261
Bst6I CTCTTC 1 cut(s) 52
BstAUI TGTACA 1 cut(s) 184
BstC8I GCNNGC 2 cut(s) 246, 347
BstDEI CTNAG 2 cut(s) 126, 167
BstF5I GGATG 3 cut(s) 70, 160, 218
BstKTI GATC 4 cut(s) 20, 200, 240, 359
BstMAI GTCTC 1 cut(s) 277
BstMBI GATC 4 cut(s) 17, 197, 237, 356
BstMWI GCNNNNNNNGC 5 cut(s) 169, 213, 225, 346, 351
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 282
BstSCI CCNGG 1 cut(s) 340
BstX2I RGATCY 3 cut(s) 17, 197, 237
BstYI RGATCY 3 cut(s) 17, 197, 237
BsuRI GGCC 1 cut(s) 172
BtsCI GGATG 3 cut(s) 70, 160, 218
BtsIMutI CAGTG 1 cut(s) 164
Cac8I GCNNGC 2 cut(s) 246, 347
Cfr10I RCCGGY 1 cut(s) 244
Csp6I GTAC 1 cut(s) 185
CviAII CATG 1 cut(s) 279
CviQI GTAC 1 cut(s) 185
DdeI CTNAG 2 cut(s) 126, 167
DpnI GATC 4 cut(s) 19, 199, 239, 358
DpnII GATC 4 cut(s) 17, 197, 237, 356
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
EciI GGCGGA 1 cut(s) 222
Ecl136II GAGCTC 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 230
Eco31I GGTCTC 1 cut(s) 277
Eco53kI GAGCTC 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 33
EcoICRI GAGCTC 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 230
FaeI CATG 1 cut(s) 282
FaiI YATR 2 cut(s) 146, 280
FatI CATG 1 cut(s) 278
Fnu4HI GCNGC 1 cut(s) 217
FokI GGATG 3 cut(s) 77, 167, 225
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 1 cut(s) 217
GluI GCNGC 1 cut(s) 217
HaeIII GGCC 1 cut(s) 172
HapII CCGG 3 cut(s) 81, 223, 245
Hin1II CATG 1 cut(s) 282
HpaII CCGG 3 cut(s) 81, 223, 245
Hpy188I TCNGA 2 cut(s) 35, 127
Hpy188III TCNNGA 2 cut(s) 81, 178
Hpy99I CGWCG 1 cut(s) 265
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 2 cut(s) 184, 261
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 2 cut(s) 13, 278
HpyF10VI GCNNNNNNNGC 5 cut(s) 169, 213, 225, 346, 351
HpyF3I CTNAG 2 cut(s) 126, 167
HpySE526I ACGT 1 cut(s) 361
Hsp92II CATG 1 cut(s) 282
Kpn2I TCCGGA 1 cut(s) 80
KroI GCCGGC 1 cut(s) 244
KroNI GCCGGC 1 cut(s) 246
Kzo9I GATC 4 cut(s) 17, 197, 237, 356
LguI GCTCTTC 1 cut(s) 52
LmnI GCTCC 2 cut(s) 225, 332
LpnPI CCDG 9 cut(s) 36, 55, 94, 117, 216, 236, 258, 327, 354
LweI GCATC 1 cut(s) 203
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 1 cut(s) 362
MalI GATC 4 cut(s) 19, 199, 239, 358
MboI GATC 4 cut(s) 17, 197, 237, 356
MboII GAAGA 3 cut(s) 12, 39, 247
MflI RGATCY 3 cut(s) 17, 197, 237
MhlI GDGCHC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 4 cut(s) 121, 162, 203, 319
MroI TCCGGA 1 cut(s) 80
MroNI GCCGGC 1 cut(s) 244
MseI TTAA 2 cut(s) 150, 159
MspI CCGG 3 cut(s) 81, 223, 245
MspR9I CCNGG 1 cut(s) 342
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 5 cut(s) 169, 213, 225, 346, 351
NaeI GCCGGC 1 cut(s) 246
NdeII GATC 4 cut(s) 17, 197, 237, 356
NgoMIV GCCGGC 1 cut(s) 244
NlaIII CATG 1 cut(s) 282
NspI RCATGY 1 cut(s) 282
PciSI GCTCTTC 1 cut(s) 52
PdiI GCCGGC 1 cut(s) 246
PkrI GCNGC 1 cut(s) 218
Psp124BI GAGCTC 1 cut(s) 230
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PsuI RGATCY 3 cut(s) 17, 197, 237
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
SacI GAGCTC 1 cut(s) 230
SapI GCTCTTC 1 cut(s) 52
SaqAI TTAA 2 cut(s) 150, 159
SatI GCNGC 1 cut(s) 217
Sau3AI GATC 4 cut(s) 17, 197, 237, 356
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 1 cut(s) 230
SetI ASST 6 cut(s) 74, 90, 125, 230, 273, 364
SfaNI GCATC 1 cut(s) 203
SsiI CCGC 3 cut(s) 207, 216, 323
SstI GAGCTC 1 cut(s) 230
StyD4I CCNGG 1 cut(s) 340
TaaI ACNGT 2 cut(s) 184, 261
TaiI ACGT 1 cut(s) 364
TatI WGTACW 1 cut(s) 184
TauI GCSGC 1 cut(s) 219
Tru1I TTAA 2 cut(s) 150, 159
Tru9I TTAA 2 cut(s) 150, 159
TscAI CASTG 1 cut(s) 171
TspRI CASTG 1 cut(s) 171
XceI RCATGY 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.