Rorug02G0330700

Domain of unknown function (DUF4666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004606
Physical Location & Seq
Forward (+)
40768819 .. 40769582
764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0330700.1

Sequence Viewer

Length: 678 bp
ATGCACGTTAGATATGCAGGAATGGTTCATTCTTTGGCAATTCAGATGGGTTTTGGATTGGATGTGTATTTTGGCAGTACTATGATTGAGGTGTGTGTGAAATGCGGCAATCTCCGTAAGTTGTTTGATGAAATGCTTAACAGAGATTTAGTTTCTCGGACTTCGATGATTTCCGGGTATGTTTCTGAAGGAAATGTTGCTTTTGGGTTTAGTTTGTTCAATGAAATGAGGATGGAATTGGAACCGAATTCGGTGACAATGTTGGTCATGTTGCAAGGGTGTTGTGGTTTTGAAACTTCAATATATGGGAGGCAAGTTCATGGACATGTGATTAAGAATGGTTTGCTAAGTAATGGAGCTGTTCAGAACTCAATCTTGGGAATGTCTGCTAAATTAGGGGATTTTGTAAAAGTGAGAGATTTGTTCAATGAGATGCAGGGTGAAGTAGCACCAAGTATTGAGACATTAACCACAGTTATAACAGCATTGACCAAACATGGGATTCTATCCCAAGGTGAAAGCTTGCATGGTTTAGCAATAAAAACTGGTCTTCATGATGATGTTTTGCAGACATCTTTTTTGGACTTTTATGCCAAATGTGGAAAATTGGAAAGCTCAGCTCGGATAAATTATTTAGAGAACTCCTTGATAGAAACTGCATTACTTGCGGTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

225

Amino Acids

24.66

Weight (kDa)

5.78

Isoelectric Point (pI)

26.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 49 - 95 6e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 479
AciI CCGC 2 cut(s) 105, 668
AcsI RAATTY 1 cut(s) 247
AcuI CTGAAG 1 cut(s) 207
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 1 cut(s) 498
AflIII ACRYGT 1 cut(s) 325
AgsI TTSAA 4 cut(s) 220, 293, 300, 427
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AluBI AGCT 4 cut(s) 359, 522, 615, 620
AluI AGCT 4 cut(s) 359, 522, 615, 620
Alw26I GTCTC 1 cut(s) 455
ApoI RAATTY 1 cut(s) 247
ArsI GACNNNNNNTTYG 2 cut(s) 562, 594
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 3 cut(s) 265, 452, 527
BbsI GAAGAC 1 cut(s) 542
BccI CCATC 2 cut(s) 40, 226
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 1 cut(s) 455
BisI GCNGC 1 cut(s) 106
BlpI GCTNAGC 1 cut(s) 616
BlsI GCNGC 1 cut(s) 107
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 175
BmiI GGNNCC 1 cut(s) 243
BmrFI CCNGG 1 cut(s) 175
BmsI GCATC 1 cut(s) 423
BpiI GAAGAC 1 cut(s) 542
Bpu1102I GCTNAGC 1 cut(s) 616
BpuMI CCSGG 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 511
Bsc4I CCNNNNNNNGG 1 cut(s) 498
Bse1I ACTGG 1 cut(s) 550
BseDI CCNNGG 1 cut(s) 511
BseGI GGATG 2 cut(s) 67, 237
BseLI CCNNNNNNNGG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 630
BseNI ACTGG 1 cut(s) 550
BsiSI CCGG 1 cut(s) 174
BslI CCNNNNNNNGG 1 cut(s) 498
BsmAI GTCTC 1 cut(s) 455
Bsp1720I GCTNAGC 1 cut(s) 616
BspACI CCGC 2 cut(s) 105, 668
BspCNI CTCAG 1 cut(s) 629
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 1 cut(s) 243
BsrI ACTGG 1 cut(s) 550
BssECI CCNNGG 1 cut(s) 511
BssT1I CCWWGG 1 cut(s) 511
Bst4CI ACNGT 1 cut(s) 475
BstAPI GCANNNNNTGC 1 cut(s) 665
BstC8I GCNNGC 1 cut(s) 524
BstDEI CTNAG 2 cut(s) 347, 616
BstF5I GGATG 2 cut(s) 67, 237
BstMAI GTCTC 1 cut(s) 455
BstMWI GCNNNNNNNGC 1 cut(s) 665
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 1 cut(s) 173
BstV2I GAAGAC 1 cut(s) 542
BtsCI GGATG 2 cut(s) 67, 237
Cac8I GCNNGC 1 cut(s) 524
CciI TCATGA 1 cut(s) 553
Csp6I GTAC 1 cut(s) 78
CviAII CATG 6 cut(s) 268, 320, 326, 497, 527, 554
CviJI RGCY 4 cut(s) 359, 522, 615, 620
CviKI_1 RGCY 4 cut(s) 359, 522, 615, 620
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 2 cut(s) 347, 616
Eco130I CCWWGG 1 cut(s) 511
Eco57I CTGAAG 1 cut(s) 207
EcoRI GAATTC 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 511
FaeI CATG 6 cut(s) 271, 323, 329, 500, 530, 557
FatI CATG 6 cut(s) 267, 319, 325, 496, 526, 553
Fnu4HI GCNGC 1 cut(s) 106
FokI GGATG 2 cut(s) 74, 244
Fsp4HI GCNGC 1 cut(s) 106
GluI GCNGC 1 cut(s) 106
HapII CCGG 1 cut(s) 174
Hin1II CATG 6 cut(s) 271, 323, 329, 500, 530, 557
HindIII AAGCTT 1 cut(s) 520
HinfI GANTC 1 cut(s) 502
HpaII CCGG 1 cut(s) 174
HphI GGTGA 3 cut(s) 265, 452, 527
Hpy188I TCNGA 5 cut(s) 45, 159, 187, 366, 624
Hpy188III TCNNGA 1 cut(s) 554
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 1 cut(s) 475
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 8 cut(s) 4, 17, 274, 436, 526, 568, 659, 673
HpyF10VI GCNNNNNNNGC 1 cut(s) 665
HpyF3I CTNAG 2 cut(s) 347, 616
HpySE526I ACGT 1 cut(s) 6
Hsp92II CATG 6 cut(s) 271, 323, 329, 500, 530, 557
LmnI GCTCC 1 cut(s) 356
LpnPI CCDG 4 cut(s) 3, 187, 422, 531
LweI GCATC 1 cut(s) 423
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 1 cut(s) 253
MboII GAAGA 1 cut(s) 542
MluCI AATT 6 cut(s) 39, 236, 247, 392, 605, 628
MnlI CCTC 3 cut(s) 82, 222, 303
MseI TTAA 3 cut(s) 138, 333, 467
MslI CAYNNNNRTG 2 cut(s) 324, 558
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 175
MwoI GCNNNNNNNGC 1 cut(s) 665
NciI CCSGG 1 cut(s) 175
NlaIII CATG 6 cut(s) 271, 323, 329, 500, 530, 557
NlaIV GGNNCC 1 cut(s) 243
NmuCI GTSAC 1 cut(s) 253
NspI RCATGY 1 cut(s) 329
PagI TCATGA 1 cut(s) 553
PciI ACATGT 1 cut(s) 325
PfeI GAWTC 1 cut(s) 502
PkrI GCNGC 1 cut(s) 107
PscI ACATGT 1 cut(s) 325
PsiI TTATAA 1 cut(s) 479
PspN4I GGNNCC 1 cut(s) 243
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
RseI CAYNNNNRTG 2 cut(s) 324, 558
SaqAI TTAA 3 cut(s) 138, 333, 467
SatI GCNGC 1 cut(s) 106
ScaI AGTACT 1 cut(s) 79
ScrFI CCNGG 1 cut(s) 175
SetI ASST 7 cut(s) 9, 93, 361, 517, 524, 617, 622
SfaNI GCATC 1 cut(s) 423
SmiMI CAYNNNNRTG 2 cut(s) 324, 558
Sse9I AATT 6 cut(s) 39, 236, 247, 392, 605, 628
SsiI CCGC 2 cut(s) 105, 668
StyD4I CCNGG 1 cut(s) 173
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 1 cut(s) 475
TaiI ACGT 1 cut(s) 9
TaqI TCGA 1 cut(s) 164
TasI AATT 6 cut(s) 39, 236, 247, 392, 605, 628
TatI WGTACW 1 cut(s) 77
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 1 cut(s) 502
Tru1I TTAA 3 cut(s) 138, 333, 467
Tru9I TTAA 3 cut(s) 138, 333, 467
TseFI GTSAC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 5 cut(s) 17, 144, 237, 308, 542
TspGWI ACGGA 1 cut(s) 104
XapI RAATTY 1 cut(s) 247
XceI RCATGY 1 cut(s) 329
ZrmI AGTACT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.