AT5G22750

HIRAN

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002288
Physical Location & Seq
Reverse (-)
7565037 .. 7571109
6073 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22750.1

Sequence Viewer

Length: 3090 bp
ATGGGAACGAAAGTCTCAGACGATCTTGTTTCCACCGTCAGATCAGTCGTGGGTTCCGATTACTCAGATATGGATATAATCAGGGCTTTACACATGGCGAATCATGATCCAACGGCTGCTATCAATATAATCTTCGACACTCCAAGTTTCGCCAAACCTGATGTAGCCACTCCTACCCCGAGCGGCTCTAATGGAGGGAAGCGAGTTGATAGTGGATTAAAGGGCTGTACTTTTGGTGACAGCGGAAGTGTTGGAGCGAATCATCGCGTGGAGGAAGAAAATGAGAGTGTTAATGGTGGAGGAGAAGAGAGTGTTTCAGGGAATGAGTGGTGGTTTGTTGGTTGTTCTGAATTGGCTGGGTTATCGACATGTAAAGGAAGGAAATTGAAGTCTGGTGATGAATTGGTGTTCACGTTTCCGCATAGTAAAGGATTAAAGCCTGAGACTACGCCTGGGAAGCGCGGTTTTGGGCGGGGAAGGCCAGCTTTGCGTGGTGCTTCTGATATCGTTAGGTTCTCTACAAAGGATTCAGGAGAGATTGGTAGAATACCAAACGAGTGGGCTCGGTGTCTTCTACCACTTGTGAGAGACAAGAAAATTAGGATAGAAGGCAGTTGCAAGTCGGCGCCTGAAGCTTTGAGCATCATGGATACAATTCTTCTGTCTGTAAGCGTGTACATTAATAGTTCCATGTTTCAAAAGCATAGTGCGACTTCATTTAAGACAGCTAGTAATACGGCAGAGGAATCAATGTTCCATCCTCTCCCAAATCTCTTTCGGTTACTCGGTTTGATCCCCTTTAAGAAGGCAGAGTTTACTCCAGAGGATTTTTACTCTAAGAAGCGACCTTTGAGTTCCAAGGATGGTTCTGCTATTCCTACTTCGTTGCTTCAATTAAACAAGGTCAAGAATATGAATCAAGATGCAAACGGAGATGAAAATGAGCAGTGTATCAGCGATGGTGATCTTGATAACATTGTTGGTGTTGGGGACAGTTCTGGATTAAAGGAAATGGAAACTCCACATACACTTCTGTGTGAGCTTCGTCCATACCAAAAGCAGGCACTTCATTGGATGACCCAACTGGAGAAAGGAAATTGCACTGATGAGGCAGCAACAATGCTTCACCCGTGTTGGGAAGCATACTGTTTAGCAGACAAGAGGGAACTGGTTGTCTACCTGAATTCTTTTACTGGTGATGCTACAATACACTTCCCTAGCACACTTCAAATGGCAAGAGGAGGAATATTAGCAGACGCAATGGGTCTTGGAAAGACTGTAATGACCATATCCCTTTTGCTTGCCCATTCTTGGAAAGCTGCATCAACTGGGTTTCTATGCCCCAACTATGAAGGAGACAAAGTGATCAGCAGTTCTGTAGATGATCTCACTAGTCCCCCGGTGAAGGCAACCAAATTTCTAGGCTTTGATAAGAGGCTTCTTGAACAAAAAAGTGTACTTCAAAATGGTGGTAACCTGATTGTATGTCCGATGACACTTTTAGGACAGTGGAAGACAGAGATTGAAATGCATGCAAAGCCTGGGTCTCTATCTGTCTATGTTCACTATGGGCAAAGCAGGCCGAAGGATGCAAAACTTCTTTCCCAGAGTGATGTGGTAATCACCACATATGGAGTTCTAACATCCGAATTCTCGCAAGAGAACTCAGCAGACCATGAAGGAATTTATGCAGTTCGATGGTTTAGGATTGTTCTTGACGAGGCACATACCATCAAAAACTCAAAAAGCCAAATTTCCTTGGCTGCTGCAGCTCTGGTTGCTGATAGGCGTTGGTGTCTTACGGGTACTCCTATTCAGAACAATCTGGAGGATTTATACAGCCTTCTACGGTTTTTGAGGATTGAACCATGGGGAACTTGGGCATGGTGGAATAAACTTGTCCAAAAGCCATTTGAAGAGGGTGATGAGAGAGGGTTAAAGCTAGTGCAGTCTATCTTAAAACCTATCATGCTTAGGAGAACAAAGTCTAGCACAGACCGAGAAGGAAGGCCGATTCTTGTTCTACCCCCTGCTGATGCACGGGTCATTTACTGTGAACTTTCGGAGTCTGAGAGGGATTTCTACGACGCGCTATTTAAAAGATCCAAGGTCAAATTTGATCAATTTGTTGAACAAGGCAAAGTTCTTCATAACTATGCTTCGATCCTGGAACTGCTTTTGCGTCTTCGACAATGTTGTGATCACCCATTTTTAGTAATGAGTCGAGGGGATACAGCGGAATACTCTGATCTGAATAAGCTTTCTAAACGTTTCCTTAGTGGAAAGTCTTCTGGCTTAGAAAGGGAAGGAAAAGATGTACCGTCAGAGGCTTTTGTTCAGGAGGTGGTAGAGGAACTGCGCAAAGGAGAGCAAGGAGAGTGTCCAATATGCCTTGAAGCACTTGAGGATGCTGTATTAACGCCATGTGCTCATAGATTATGTCGTGAGTGTCTCTTGGCAAGTTGGAGAAATTCTACTTCTGGGTTATGTCCTGTGTGTAGGAACACTGTAAGCAAACAAGAACTCATCACAGCACCAACCGAAAGTAGATTCCAGGTTGACGTGGAAAAGAATTGGGTGGAATCATCGAAAATCACTGCTCTTCTGGAAGAGCTTGAAGGTCTTCGTTCTTCAGGCTCTAAGAGCATTCTCTTTAGCCAGTGGACCGCTTTCCTCGATCTCCTCCAAATTCCCCTCTCTCGGAATAACTTTTCATTTGTCCGTCTTGATGGCACGCTAAGTCAGCAGCAACGAGAGAAGGTCCTTAAAGAATTTTCCGAAGATGGCAGTATCCTGGTACTGTTGATGTCTCTAAAAGCTGGTGGCGTTGGGATAAATCTAACAGCTGCGTCCAATGCTTTTGTCATGGATCCATGGTGGAACCCAGCGGTAGAGGAACAAGCTGTTATGCGTATTCATCGTATAGGGCAAACTAAGGAAGTCAAAATCAGAAGATTCATCGTTAAGGGAACGGTTGAAGAGAGAATGGAGGCGGTTCAGGCGAGGAAGCAGAGAATGATCTCTGGGGCTTTAACCGATCAAGAAGTACGAAGTGCACGTATAGAGGAACTCAAGATGTTATTTACCTGA

Protein Analysis

1029

Amino Acids

114.29

Weight (kDa)

6.4

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_RAD5A PF24559 5 - 49 3.2e-27 DNA repair protein RAD5A UBA domain
HIRAN PF08797 114 - 223 2.7e-16 HIRAN domain
SNF2-rel_dom PF00176 351 - 737 8.1e-79 SNF2-related domain
DEAD PF00270 422 - 611 1.5e-06 DEAD/DEAH box helicase
zf-C3HC4_3 PF13920 791 - 839 2.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 793 - 833 8.7e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 793 - 834 7.6e-08 Ring finger domain
zf-C3HC4 PF00097 794 - 833 4e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 794 - 831 1.5e-08 RING-type zinc-finger
Prok-RING_4 PF14447 794 - 840 8e-06 Prokaryotic RING finger family 4
zf-C3HC4_4 PF15227 794 - 833 5.5e-06 zinc finger of C3HC4-type, RING
Helicase_C PF00271 864 - 976 2.8e-21 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2360
AccB1I GGYRCC 1 cut(s) 625
AccBSI CCGCTC 1 cut(s) 183
AccI GTMKAC 1 cut(s) 1176
AccII CGCG 3 cut(s) 267, 462, 2090
AciI CCGC 9 cut(s) 183, 243, 419, 462, 472, 2237, 2667, 2888, 2993
AclI AACGTT 1 cut(s) 2269
AclWI GGATC 6 cut(s) 101, 787, 2097, 2158, 2864, 2877
AcsI RAATTY 9 cut(s) 1183, 1415, 1649, 1683, 1752, 2114, 2470, 2688, 2771
AcuI CTGAAG 2 cut(s) 651, 2616
AcyI GRCGYC 1 cut(s) 626
AfaI GTAC 7 cut(s) 229, 677, 1458, 1807, 2319, 2799, 3048
AfiI CCNNNNNNNGG 5 cut(s) 1060, 1135, 1311, 1405, 2700
AflIII ACRYGT 1 cut(s) 368
AhlI ACTAGT 1 cut(s) 1391
AjiI CACGTC 1 cut(s) 2563
AjnI CCWGG 5 cut(s) 451, 1540, 2166, 2553, 2793
AleI CACNNNNGTG 1 cut(s) 1033
AloI GAACNNNNNNTCC 2 cut(s) 737, 769
Alw21I GWGCWC 2 cut(s) 2431, 3058
Alw26I GTCTC 7 cut(s) 19, 437, 582, 1350, 1551, 2456, 2814
Alw44I GTGCAC 1 cut(s) 3054
AlwI GGATC 6 cut(s) 101, 787, 2097, 2158, 2864, 2877
Ama87I CYCGRG 1 cut(s) 178
AoxI GGCC 3 cut(s) 479, 1580, 2009
ApaLI GTGCAC 1 cut(s) 3054
ApeKI GCWGC 8 cut(s) 116, 1112, 1319, 1763, 1766, 1769, 2746, 2846
ApoI RAATTY 9 cut(s) 1183, 1415, 1649, 1683, 1752, 2114, 2470, 2688, 2771
ArsI GACNNNNNNTTYG 2 cut(s) 1529, 1561
AseI ATTAAT 1 cut(s) 681
Asp700I GAANNNNTTC 2 cut(s) 2287, 2622
AspLEI GCGC 4 cut(s) 462, 628, 2092, 2361
AspS9I GGNCC 2 cut(s) 2664, 2761
AsuC2I CCSGG 1 cut(s) 1400
AsuHPI GGTGA 9 cut(s) 248, 407, 974, 1118, 1208, 1414, 1615, 1934, 2195
AvaI CYCGRG 1 cut(s) 178
AvaII GGWCC 2 cut(s) 2664, 2761
BaeGI GKGCMC 1 cut(s) 3058
BamHI GGATCC 1 cut(s) 2869
BanI GGYRCC 1 cut(s) 625
BanII GRGCYC 1 cut(s) 565
BbsI GAAGAC 5 cut(s) 563, 1520, 2177, 2280, 2615
Bbv12I GWGCWC 2 cut(s) 2431, 3058
BbvI GCAGC 8 cut(s) 103, 1124, 1306, 1750, 1753, 1781, 2758, 2833
BccI CCATC 7 cut(s) 765, 857, 953, 1692, 1739, 2723, 2777
BceAI ACGGC 2 cut(s) 129, 753
BciT130I CCWGG 5 cut(s) 453, 1542, 2168, 2555, 2795
BciVI GTATCC 3 cut(s) 643, 2224, 2801
BclI TGATCA 3 cut(s) 1365, 2119, 2200
BcnI CCSGG 1 cut(s) 1400
BcoDI GTCTC 7 cut(s) 19, 437, 582, 1350, 1551, 2456, 2814
BcuI ACTAGT 1 cut(s) 1391
BfaI CTAG 6 cut(s) 729, 1218, 1392, 1421, 1943, 1989
BfmI CTRYAG 2 cut(s) 1377, 1767
BfoI RGCGCY 1 cut(s) 629
BfuI GTATCC 3 cut(s) 643, 2224, 2801
BisI GCNGC 9 cut(s) 117, 184, 1113, 1320, 1764, 1767, 1770, 2747, 2847
BlsI GCNGC 9 cut(s) 118, 185, 1114, 1321, 1765, 1768, 1771, 2748, 2848
Bme1390I CCNGG 6 cut(s) 453, 1400, 1542, 2168, 2555, 2795
Bme18I GGWCC 2 cut(s) 2664, 2761
BmeT110I CYCGRG 1 cut(s) 178
BmgBI CACGTC 1 cut(s) 2563
BmgT120I GGNCC 2 cut(s) 2664, 2761
BmiI GGNNCC 4 cut(s) 55, 627, 2871, 2882
BmrFI CCNGG 6 cut(s) 453, 1400, 1542, 2168, 2555, 2795
BmrI ACTGGG 1 cut(s) 1338
BmsI GCATC 7 cut(s) 651, 913, 1189, 1331, 1579, 2026, 2398
BmuI ACTGGG 1 cut(s) 1338
BpiI GAAGAC 5 cut(s) 563, 1520, 2177, 2280, 2615
BpmI CTGGAG 3 cut(s) 804, 1106, 1847
Bpu10I CCTNAGC 1 cut(s) 1973
BpuEI CTTGAG 2 cut(s) 2423, 3056
BpuMI CCSGG 1 cut(s) 1400
BsaAI YACGTR 1 cut(s) 3059
BsaBI GATNNNNATC 1 cut(s) 963
BsaHI GRCGYC 1 cut(s) 626
BsaI GGTCTC 1 cut(s) 1551
BsaJI CCNNGG 8 cut(s) 452, 858, 1398, 1541, 1758, 1868, 2106, 2873
BsaXI ACNNNNNCTCC 4 cut(s) 1347, 1377, 1792, 1822
Bsc4I CCNNNNNNNGG 5 cut(s) 1060, 1135, 1311, 1405, 2700
Bse1I ACTGG 5 cut(s) 1089, 1173, 1198, 1333, 2659
Bse3DI GCAATG 1 cut(s) 1266
Bse8I GATNNNNATC 1 cut(s) 963
BseBI CCWGG 5 cut(s) 453, 1542, 2168, 2555, 2795
BseDI CCNNGG 8 cut(s) 452, 858, 1398, 1541, 1758, 1868, 2106, 2873
BseGI GGATG 6 cut(s) 757, 868, 1080, 1594, 1643, 2413
BseJI GATNNNNATC 1 cut(s) 963
BseLI CCNNNNNNNGG 5 cut(s) 1060, 1135, 1311, 1405, 2700
BseMI GCAATG 1 cut(s) 1266
BseMII CTCAG 5 cut(s) 30, 78, 432, 1680, 2061
BseNI ACTGG 5 cut(s) 1089, 1173, 1198, 1333, 2659
BseRI GAGGAG 3 cut(s) 315, 1254, 2672
BseSI GKGCMC 1 cut(s) 3058
BseXI GCAGC 8 cut(s) 103, 1124, 1306, 1750, 1753, 1781, 2758, 2833
BseYI CCCAGC 2 cut(s) 356, 2884
BsgI GTGCAG 1 cut(s) 1967
Bsh1236I CGCG 3 cut(s) 267, 462, 2090
BshFI GGCC 3 cut(s) 481, 1582, 2011
BshNI GGYRCC 1 cut(s) 625
BsiHKAI GWGCWC 2 cut(s) 2431, 3058
BsiHKCI CYCGRG 1 cut(s) 178
BsiSI CCGG 1 cut(s) 1400
BslFI GGGAC 2 cut(s) 1004, 1380
BslI CCNNNNNNNGG 5 cut(s) 1060, 1135, 1311, 1405, 2700
BsmAI GTCTC 7 cut(s) 19, 437, 582, 1350, 1551, 2456, 2814
BsmFI GGGAC 2 cut(s) 1004, 1380
BsmI GAATGC 1 cut(s) 2646
BsnI GGCC 3 cut(s) 481, 1582, 2011
Bso31I GGTCTC 1 cut(s) 1551
BsoBI CYCGRG 1 cut(s) 178
Bsp1286I GDGCHC 3 cut(s) 565, 2431, 3058
Bsp1407I TGTACA 1 cut(s) 675
Bsp19I CCATGG 2 cut(s) 1868, 2873
BspACI CCGC 9 cut(s) 183, 243, 419, 462, 472, 2237, 2667, 2888, 2993
BspANI GGCC 3 cut(s) 481, 1582, 2011
BspCNI CTCAG 5 cut(s) 29, 77, 433, 1679, 2062
BspFNI CGCG 3 cut(s) 267, 462, 2090
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 4 cut(s) 55, 627, 2871, 2882
BspMAI CTGCAG 1 cut(s) 1771
BspPI GGATC 6 cut(s) 101, 787, 2097, 2158, 2864, 2877
BspQI GCTCTTC 2 cut(s) 2604, 2607
BspT107I GGYRCC 1 cut(s) 625
BspTNI GGTCTC 1 cut(s) 1551
BsrBI CCGCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 1266
BsrGI TGTACA 1 cut(s) 675
BsrI ACTGG 5 cut(s) 1089, 1173, 1198, 1333, 2659
BssECI CCNNGG 8 cut(s) 452, 858, 1398, 1541, 1758, 1868, 2106, 2873
BssNI GRCGYC 1 cut(s) 626
BssT1I CCWWGG 5 cut(s) 858, 1758, 1868, 2106, 2873
Bst2UI CCWGG 5 cut(s) 453, 1542, 2168, 2555, 2795
Bst6I CTCTTC 5 cut(s) 300, 1911, 2604, 2607, 2973
BstACI GRCGYC 1 cut(s) 626
BstAUI TGTACA 1 cut(s) 675
BstBAI YACGTR 1 cut(s) 3059
BstC8I GCNNGC 6 cut(s) 483, 1062, 1302, 1533, 1580, 2735
BstDSI CCRYGG 2 cut(s) 1868, 2873
BstEII GGTNACC 1 cut(s) 1472
BstF5I GGATG 6 cut(s) 757, 868, 1080, 1594, 1643, 2413
BstFNI CGCG 3 cut(s) 267, 462, 2090
BstH2I RGCGCY 1 cut(s) 629
BstHHI GCGC 4 cut(s) 462, 628, 2092, 2361
BstMAI GTCTC 7 cut(s) 19, 437, 582, 1350, 1551, 2456, 2814
BstNI CCWGG 5 cut(s) 453, 1542, 2168, 2555, 2795
BstNSI RCATGY 2 cut(s) 372, 1535
BstPI GGTNACC 1 cut(s) 1472
BstSCI CCNGG 6 cut(s) 451, 1398, 1540, 2166, 2553, 2793
BstSFI CTRYAG 2 cut(s) 1377, 1767
BstSLI GKGCMC 1 cut(s) 3058
BstUI CGCG 3 cut(s) 267, 462, 2090
BstV1I GCAGC 8 cut(s) 103, 1124, 1306, 1750, 1753, 1781, 2758, 2833
BstV2I GAAGAC 5 cut(s) 563, 1520, 2177, 2280, 2615
BstX2I RGATCY 2 cut(s) 2102, 2869
BstXI CCANNNNNNTGG 1 cut(s) 558
BstYI RGATCY 2 cut(s) 2102, 2869
BsuI GTATCC 3 cut(s) 643, 2224, 2801
BsuRI GGCC 3 cut(s) 481, 1582, 2011
BtgI CCRYGG 2 cut(s) 1868, 2873
BtgZI GCGATG 2 cut(s) 248, 972
BtrI CACGTC 1 cut(s) 2563
BtsCI GGATG 6 cut(s) 757, 868, 1080, 1594, 1643, 2413
BtsI GCAGTG 2 cut(s) 953, 2595
BtsIMutI CAGTG 6 cut(s) 953, 1101, 1514, 2505, 2595, 2666
Cac8I GCNNGC 6 cut(s) 483, 1062, 1302, 1533, 1580, 2735
CciI TCATGA 1 cut(s) 103
CfoI GCGC 4 cut(s) 462, 628, 2092, 2361
Cfr13I GGNCC 2 cut(s) 2664, 2761
CseI GACGC 4 cut(s) 1265, 2096, 2171, 2838
Csp6I GTAC 7 cut(s) 228, 676, 1457, 1806, 2318, 2798, 3047
CviQI GTAC 7 cut(s) 228, 676, 1457, 1806, 2318, 2798, 3047
DinI GGCGCC 1 cut(s) 627
DraI TTTAAA 1 cut(s) 2098
Eam1104I CTCTTC 5 cut(s) 300, 1911, 2604, 2607, 2973
EarI CTCTTC 5 cut(s) 300, 1911, 2604, 2607, 2973
Eco130I CCWWGG 5 cut(s) 858, 1758, 1868, 2106, 2873
Eco24I GRGCYC 1 cut(s) 565
Eco31I GGTCTC 1 cut(s) 1551
Eco32I GATATC 1 cut(s) 505
Eco47I GGWCC 2 cut(s) 2664, 2761
Eco57I CTGAAG 2 cut(s) 651, 2616
Eco88I CYCGRG 1 cut(s) 178
Eco91I GGTNACC 1 cut(s) 1472
EcoO109I RGGNCCY 1 cut(s) 2761
EcoO65I GGTNACC 1 cut(s) 1472
EcoRI GAATTC 2 cut(s) 1183, 1649
EcoRII CCWGG 5 cut(s) 451, 1540, 2166, 2553, 2793
EcoRV GATATC 1 cut(s) 505
EcoT14I CCWWGG 5 cut(s) 858, 1758, 1868, 2106, 2873
EcoT22I ATGCAT 1 cut(s) 1533
EcoT38I GRGCYC 1 cut(s) 565
EgeI GGCGCC 1 cut(s) 627
EheI GGCGCC 1 cut(s) 627
ErhI CCWWGG 5 cut(s) 858, 1758, 1868, 2106, 2873
FalI AAGNNNNNCTT 2 cut(s) 469, 501
FaqI GGGAC 2 cut(s) 1004, 1380
FauI CCCGC 1 cut(s) 465
FauNDI CATATG 1 cut(s) 1630
FbaI TGATCA 3 cut(s) 1365, 2119, 2200
FblI GTMKAC 1 cut(s) 1176
Fnu4HI GCNGC 9 cut(s) 117, 184, 1113, 1320, 1764, 1767, 1770, 2747, 2847
FokI GGATG 6 cut(s) 744, 875, 1087, 1601, 1630, 2420
FriOI GRGCYC 1 cut(s) 565
Fsp4HI GCNGC 9 cut(s) 117, 184, 1113, 1320, 1764, 1767, 1770, 2747, 2847
FspBI CTAG 6 cut(s) 729, 1218, 1392, 1421, 1943, 1989
FspI TGCGCA 1 cut(s) 2360
GlaI GCGC 4 cut(s) 461, 627, 2091, 2360
GluI GCNGC 9 cut(s) 117, 184, 1113, 1320, 1764, 1767, 1770, 2747, 2847
GsaI CCCAGC 2 cut(s) 360, 2888
GsuI CTGGAG 3 cut(s) 804, 1106, 1847
HaeII RGCGCY 1 cut(s) 629
HaeIII GGCC 3 cut(s) 481, 1582, 2011
HapII CCGG 1 cut(s) 1400
HgaI GACGC 4 cut(s) 1265, 2096, 2171, 2838
HhaI GCGC 4 cut(s) 462, 628, 2092, 2361
Hin1I GRCGYC 1 cut(s) 626
Hin6I GCGC 4 cut(s) 460, 626, 2090, 2359
HinP1I GCGC 4 cut(s) 460, 626, 2090, 2359
HincII GTYRAC 1 cut(s) 2560
HindII GTYRAC 1 cut(s) 2560
HindIII AAGCTT 2 cut(s) 633, 2258
HpaII CCGG 1 cut(s) 1400
HphI GGTGA 9 cut(s) 248, 407, 974, 1118, 1208, 1414, 1615, 1934, 2195
Hpy99I CGWCG 1 cut(s) 2090
HpyCH4IV ACGT 4 cut(s) 413, 2269, 2562, 3058
HpySE526I ACGT 4 cut(s) 413, 2269, 2562, 3058
Hsp92I GRCGYC 1 cut(s) 626
HspAI GCGC 4 cut(s) 460, 626, 2090, 2359
KasI GGCGCC 1 cut(s) 625
Ksp22I TGATCA 3 cut(s) 1365, 2119, 2200
LguI GCTCTTC 2 cut(s) 2604, 2607
LmnI GCTCC 1 cut(s) 254
Lsp1109I GCAGC 8 cut(s) 103, 1124, 1306, 1750, 1753, 1781, 2758, 2833
LweI GCATC 7 cut(s) 651, 913, 1189, 1331, 1579, 2026, 2398
MaeI CTAG 6 cut(s) 729, 1218, 1392, 1421, 1943, 1989
MaeII ACGT 4 cut(s) 413, 2269, 2562, 3058
MaeIII GTNAC 3 cut(s) 236, 780, 1472
MbiI CCGCTC 1 cut(s) 183
MflI RGATCY 2 cut(s) 2102, 2869
MhlI GDGCHC 3 cut(s) 565, 2431, 3058
Mly113I GGCGCC 1 cut(s) 626
MlyI GAGTC 2 cut(s) 2075, 2230
MmeI TCCRAC 3 cut(s) 134, 232, 2444
Mph1103I ATGCAT 1 cut(s) 1533
MroXI GAANNNNTTC 2 cut(s) 2287, 2622
MslI CAYNNNNRTG 2 cut(s) 1033, 2154
MspA1I CMGCKG 4 cut(s) 243, 2237, 2846, 2888
MspI CCGG 1 cut(s) 1400
MspR9I CCNGG 6 cut(s) 453, 1400, 1542, 2168, 2555, 2795
Mva1269I GAATGC 1 cut(s) 2646
MvaI CCWGG 5 cut(s) 453, 1542, 2168, 2555, 2795
MvnI CGCG 3 cut(s) 267, 462, 2090
NarI GGCGCC 1 cut(s) 626
NciI CCSGG 1 cut(s) 1400
NcoI CCATGG 2 cut(s) 1868, 2873
NdeI CATATG 1 cut(s) 1630
NlaIV GGNNCC 4 cut(s) 55, 627, 2871, 2882
NmuCI GTSAC 1 cut(s) 236
NsbI TGCGCA 1 cut(s) 2360
NsiI ATGCAT 1 cut(s) 1533
NspI RCATGY 2 cut(s) 372, 1535
OliI CACNNNNGTG 1 cut(s) 1033
PaeI GCATGC 1 cut(s) 1535
PagI TCATGA 1 cut(s) 103
PciI ACATGT 1 cut(s) 368
PciSI GCTCTTC 2 cut(s) 2604, 2607
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 3055
PctI GAATGC 1 cut(s) 2646
PdmI GAANNNNTTC 2 cut(s) 2287, 2622
PfeI GAWTC 9 cut(s) 100, 259, 527, 746, 916, 2014, 2550, 2582, 2955
PfoI TCCNGGA 1 cut(s) 2166
PkrI GCNGC 9 cut(s) 118, 185, 1114, 1321, 1765, 1768, 1771, 2748, 2848
PleI GAGTC 2 cut(s) 2074, 2229
PluTI GGCGCC 1 cut(s) 629
PpsI GAGTC 2 cut(s) 2074, 2229
Ppu21I YACGTR 1 cut(s) 3059
PpuMI RGGWCCY 1 cut(s) 2761
PscI ACATGT 1 cut(s) 368
PshBI ATTAAT 1 cut(s) 681
Psp1406I AACGTT 1 cut(s) 2269
Psp5II RGGWCCY 1 cut(s) 2761
Psp6I CCWGG 5 cut(s) 451, 1540, 2166, 2553, 2793
PspEI GGTNACC 1 cut(s) 1472
PspFI CCCAGC 2 cut(s) 356, 2884
PspGI CCWGG 5 cut(s) 451, 1540, 2166, 2553, 2793
PspN4I GGNNCC 4 cut(s) 55, 627, 2871, 2882
PspPI GGNCC 2 cut(s) 2664, 2761
PspPPI RGGWCCY 1 cut(s) 2761
PstI CTGCAG 1 cut(s) 1771
PsuI RGATCY 2 cut(s) 2102, 2869
PvuII CAGCTG 1 cut(s) 2846
RsaI GTAC 7 cut(s) 229, 677, 1458, 1807, 2319, 2799, 3048
RsaNI GTAC 7 cut(s) 228, 676, 1457, 1806, 2318, 2798, 3047
RseI CAYNNNNRTG 2 cut(s) 1033, 2154
SapI GCTCTTC 2 cut(s) 2604, 2607
SatI GCNGC 9 cut(s) 117, 184, 1113, 1320, 1764, 1767, 1770, 2747, 2847
Sau96I GGNCC 2 cut(s) 2664, 2761
SchI GAGTC 2 cut(s) 2075, 2230
ScrFI CCNGG 6 cut(s) 453, 1400, 1542, 2168, 2555, 2795
SduI GDGCHC 3 cut(s) 565, 2431, 3058
SfaNI GCATC 7 cut(s) 651, 913, 1189, 1331, 1579, 2026, 2398
SfcI CTRYAG 2 cut(s) 1377, 1767
SfoI GGCGCC 1 cut(s) 627
SinI GGWCC 2 cut(s) 2664, 2761
SmiMI CAYNNNNRTG 2 cut(s) 1033, 2154
SmlI CTYRAG 2 cut(s) 2402, 3071
SmoI CTYRAG 2 cut(s) 2402, 3071
SpeI ACTAGT 1 cut(s) 1391
SphI GCATGC 1 cut(s) 1535
SsiI CCGC 9 cut(s) 183, 243, 419, 462, 472, 2237, 2667, 2888, 2993
SspDI GGCGCC 1 cut(s) 625
SspI AATATT 1 cut(s) 1248
SspMI CTAG 6 cut(s) 729, 1218, 1392, 1421, 1943, 1989
StyD4I CCNGG 6 cut(s) 451, 1398, 1540, 2166, 2553, 2793
StyI CCWWGG 5 cut(s) 858, 1758, 1868, 2106, 2873
TaiI ACGT 4 cut(s) 416, 2272, 2565, 3061
TaqI TCGA 8 cut(s) 135, 365, 1696, 2162, 2188, 2224, 2588, 2676
TaqII GACCGA 1 cut(s) 2013
TatI WGTACW 3 cut(s) 227, 675, 1456
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 9 cut(s) 100, 259, 527, 746, 916, 2014, 2550, 2582, 2955
TscAI CASTG 6 cut(s) 953, 1108, 1514, 2512, 2602, 2666
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 8 cut(s) 116, 1112, 1319, 1763, 1766, 1769, 2746, 2846
Tsp45I GTSAC 1 cut(s) 236
TspGWI ACGGA 2 cut(s) 945, 2711
TspRI CASTG 6 cut(s) 953, 1108, 1514, 2512, 2602, 2666
VneI GTGCAC 1 cut(s) 3054
VpaK11BI GGWCC 2 cut(s) 2664, 2761
VspI ATTAAT 1 cut(s) 681
XapI RAATTY 9 cut(s) 1183, 1415, 1649, 1683, 1752, 2114, 2470, 2688, 2771
XceI RCATGY 2 cut(s) 372, 1535
XcmI CCANNNNNNNNNTGG 1 cut(s) 1875
XmiI GTMKAC 1 cut(s) 1176
XmnI GAANNNNTTC 2 cut(s) 2287, 2622
XspI CTAG 6 cut(s) 729, 1218, 1392, 1421, 1943, 1989
Zsp2I ATGCAT 1 cut(s) 1533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.