Rh2BG666400

SWI SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002288
Physical Location & Seq
Reverse (-)
87894841 .. 87902006
7166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG666400.1

Sequence Viewer

Length: 2964 bp
ATGGGCAACAAAATCAGCGACGAACTATTATCCACCGTTCGTTCAATCGTCGATTCCGGCTACTCCGACATGGACATAATCCGAGCCCTACACATGGCCAACAACGACGTCACCGCCGCGATCAACATCATCTTCGACACCCCCACACCCAAATCTAAAGAAAGGTCAGATTTCGCGAGCAGTCCCAAAATCTCGGCTCCTGAAGTCGCGAATTCGAAGCCGAACGGCGGCGATTGCCCCGCCAGTTCAGGCGATGATGTAGTAGGTGACGTGGAGAGTTCGGCCGGCAGCGAGTGGTGGTTGGTGGGTTGCGGCGAGGTGGCGGGGTTATCGACGTGTAGAGGGAGGAGGATCAAGCCCGGAGACGAAGTGGATTTCACATTTCCTTTGAAGATTTCGCCGTCTCCCGGCAAGGTTTTCGGGAGGGGACGGCCGGCGGGGGCGTGTTCGGAGATTGTGAGGTTCTCCACGACGGATTCCGGAGAGATTGGACGAATACCGAAGGAATGGGCTCGGTGTCTGTTGCCACTTGTGAGAGATAAGAAGGTTAAAATCGAGGGGCATTGTAAATCTGCTCCTGATGTCTTGAGCATTATGGACACCATTCTCTTGTCTATAAGTGTGTATATCAATAGTTCTATGTTCCTTAAGCAAAAGCAGACGTTACTTAAGGCAGCCAGTAATTCCACAGAGGAAACAGTGGTTCATCCTCTCCCGACATTGTTTCAGTTGCTAGGCCTAACTCCTTTCCAGAAGGCGGAATTTACTCCTGGTGACTTGTACACGAGAAAGCGCCCTTTAGACCAAAAGGACAGTTCTGGCCTTTGTGCCTCAATTATACATGCAAATATTTCTAAAAATCCTTCTAGAAATGAAGATGAAGTTGAAAATGAGGAATGTATTTCAGATGCTGAGGTTGATAACATTGTTGGGGTCGGAGATAGCTCTGAGTTAGAGGAAATGGATCCTCCTGCTGCACTTCTGTGTGAACTTCGTCCGTATCAAAAGCAGGCTCTTCATTGGATGATCCAACTAGAAAAGGGAAAATGCATGGATAAGGCAGCAATGACTCTTCACCCTTGTTGGGAGGCATATCATCTTGCAGACAAGAGGGATCGTATTATATATTTGAACGCATTTTCTGGTGACGCAACAACAGAATTTCCAAGTGCACTTCAAATGGCCAGAGGAGGAATCCTAGCTGATGCTATGGGCCTGGGAAAGACGATTATGACAATAGCACTCCTTGTTGCTCATTCAGGACATGGATCATCAGGTAGTCAACCTGTGAGCCGGTCCTCCAGTGAAGATATTGAAGCCAGTGACATGTCAGACCATTCATCAAAACTCCCAAAGGCGGTCACAAAGTTTTCAGGATTTCATAAGTTGATGAAGCAAAAAAAAACGCTTGCATATGGGGGCAGTTTGATTGTATGTCCTATGACCCTTCTTGGCCAGTGGAAGGCAGAGATTGAATCTCATGTGCAACCTGGATCTGTATCAGTATATGTTCATTATGGACAAAGTAGACCAAGGGACGCGACTCTTTTAGCCCAAAGCGATGTTGTGATCACTACATATGGCGTTTTAGCATCAGAATTTTCTACAGAGAATTCTAAGGACAATGGGGGACTTTACTCAGTTAGTTGGTTTAGAGTGGTTCTTGATGAAGCGCACACCATAAAATCCTCTAAAAGCCAAATCTCTATTGCTGCTGCTGCTTTAGTTGCTGGTCACCGATGGTGTCTTACTGGCACCCCTATACAGAACAATCTGGAGGACATTTATAGTCTACTCCGGTTTTTGAGGGTAGAACCTTGGGCGAATTGGGCTTGGTGGAATAAACTCATCCAGAAACCCTTTGAGGAGGGTGATCAGAGAGGGTTAAGGCTGGTTCAATCTATCTTAAAGACGATTATGTTAAGGAGAACCAAGTTTAGCACTGATCGTGAAGGCAGGCCTATTTTAGTCCTTCCTCCTGCTGATATTCAGGTGGTTTACTGTGAACTCACAGAAGCTGAAAAAGACTTTTATGAGGCCTTGTTTAAAAGATCAAAGGTGAAATTTGATCAATTTGTTGAGCAAGGACGGGTTCTTCATAATTATGCATCCATATTGGAATTACTTTTACGCCTTCGCCAATGTTGTGATCACCCATTTCTTGTGATGAGTCGAGGTGATACACAAGAATATTCAGATCTGAATAAGCTTGCAAGACATTTTCTAAAAGGCAGCCAGAATTCTGTGGAAGGAGAGGCCAAAGATGTGCCTTCACGGGCTTATGTTCAGGAAGTTGTTGAAGAAATGCGCAAGGGGGAACAGGGAGAGTGTCCCATATGTCTTGAAGCATTTGAAGATGCAGTATTGACAACTTGTGCTCATCGCTTGTGCCGAGAATGTCTCTTGGCAAGTTGGCGGAATTCAACTTCTGGCTTATGTCCAGTTTGCAGGAAGAGTCTAAGTAAGCAAGATCTTATTACAGCACCAACTGACAGTCGGTTCCATGTTGATATTGAGAAAAACTGGGTGGAATCATCCAAAATAGTTATTCTTTTGCGTGAACTTGAATGTCTTCGTTCGTCAGGCTCAAAGAGCATTGTCTTCAGCCAGTGGACGGCCTTTCTGGACCTGCTGCAGATACCTCTTTCTCGGAGTAACATTCCATTTTTGCGCCTGGATGGGACTCTGAATCAACAGCAGCGCGAAAAAGTGTTAAAACAGTTTTCAGAAGACAGTGACATTCTGGTGTTGCTAATGTCACTGAAGGCCGGAGGGGTTGGAATAAATTTGACAGCAGCATCCAATGCCTTTGTCTTGGATCCATGGTGGAATCCAGCTGTAGAGGAACAAGCGGTCATGCGTATTCATCGCATCGGACAAACAAAAAGTGTGATGATCAAACGATTTATCGTGAAGGTCCTGTTTCTTTTCTTCCGCCAAGTTATATATCTCTGTTTTGCGTGA

Protein Analysis

987

Amino Acids

109.62

Weight (kDa)

6.44

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_RAD5A PF24559 6 - 48 7.7e-25 DNA repair protein RAD5A UBA domain
HIRAN PF08797 103 - 206 1.1e-14 HIRAN domain
SNF2-rel_dom PF00176 334 - 720 2.2e-75 SNF2-related domain
DEAD PF00270 404 - 594 1.1e-06 DEAD/DEAH box helicase
zf-C3HC4_3 PF13920 774 - 821 5e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 776 - 817 1.1e-07 Ring finger domain
zf-C3HC4_2 PF13923 776 - 816 4.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 777 - 816 1e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 777 - 814 1.1e-08 RING-type zinc-finger
Helicase_C PF00271 848 - 959 1.5e-20 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 111
Acc16I TGCGCA 1 cut(s) 2309
Acc36I ACCTGC 1 cut(s) 2637
AccB1I GGYRCC 1 cut(s) 1754
AccI GTMKAC 2 cut(s) 1528, 1792
AccII CGCG 5 cut(s) 119, 176, 209, 1541, 2703
AccIII TCCGGA 1 cut(s) 479
AclWI GGATC 9 cut(s) 359, 959, 972, 1021, 1122, 1276, 1501, 2813, 2826
AcoI YGGCCR 5 cut(s) 96, 282, 431, 1182, 1453
AcsI RAATTY 9 cut(s) 211, 761, 1160, 1598, 1612, 2063, 2239, 2419, 2785
AcuI CTGAAG 3 cut(s) 222, 2587, 2783
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 1 cut(s) 782
AfiI CCNNNNNNNGG 8 cut(s) 94, 227, 407, 757, 1084, 1357, 1462, 2614
AflII CTTAAG 2 cut(s) 647, 668
AflIII ACRYGT 2 cut(s) 335, 1326
AjiI CACGTC 2 cut(s) 271, 336
AjnI CCWGG 4 cut(s) 769, 1215, 1489, 2673
AleI CACNNNNGTG 1 cut(s) 982
AluBI AGCT 5 cut(s) 945, 1202, 2018, 2209, 2837
AluI AGCT 5 cut(s) 945, 1202, 2018, 2209, 2837
Alw21I GWGCWC 2 cut(s) 1174, 2380
Alw26I GTCTC 3 cut(s) 357, 408, 2405
Alw44I GTGCAC 1 cut(s) 1170
AlwI GGATC 9 cut(s) 359, 959, 972, 1021, 1122, 1276, 1501, 2813, 2826
AlwNI CAGNNNCTG 2 cut(s) 911, 2018
Aor13HI TCCGGA 1 cut(s) 479
ApaLI GTGCAC 1 cut(s) 1170
ApoI RAATTY 9 cut(s) 211, 761, 1160, 1598, 1612, 2063, 2239, 2419, 2785
Asp700I GAANNNNTTC 1 cut(s) 2571
AspLEI GCGC 5 cut(s) 795, 1675, 2310, 2673, 2703
AspS9I GGNCC 4 cut(s) 1213, 1296, 2626, 2917
AsuC2I CCSGG 2 cut(s) 360, 408
AsuII TTCGAA 1 cut(s) 215
AvaII GGWCC 3 cut(s) 1296, 2626, 2917
BaeGI GKGCMC 1 cut(s) 1174
BalI TGGCCA 3 cut(s) 98, 1184, 1455
BamHI GGATCC 2 cut(s) 964, 2818
BanI GGYRCC 1 cut(s) 1754
BanII GRGCYC 2 cut(s) 88, 514
BauI CACGAG 1 cut(s) 784
BbsI GAAGAC 3 cut(s) 2564, 2593, 2736
Bbv12I GWGCWC 2 cut(s) 1174, 2380
BbvCI CCTCAGC 1 cut(s) 912
BccI CCATC 2 cut(s) 1734, 2672
BceAI ACGGC 4 cut(s) 241, 385, 446, 2631
BcgI CGANNNNNNTGC 2 cut(s) 400, 434
BciT130I CCWGG 4 cut(s) 771, 1217, 1491, 2675
BclI TGATCA 5 cut(s) 1569, 1873, 2068, 2149, 2895
BcnI CCSGG 2 cut(s) 360, 408
BcoDI GTCTC 3 cut(s) 357, 408, 2405
BfaI CTAG 4 cut(s) 734, 867, 1034, 1199
BfmI CTRYAG 3 cut(s) 1605, 2633, 2838
BfoI RGCGCY 1 cut(s) 796
BfrI CTTAAG 2 cut(s) 647, 668
BfuAI ACCTGC 1 cut(s) 2637
BglII AGATCT 2 cut(s) 2197, 2470
Bme1390I CCNGG 6 cut(s) 360, 408, 771, 1217, 1491, 2675
Bme18I GGWCC 3 cut(s) 1296, 2626, 2917
BmgBI CACGTC 2 cut(s) 271, 336
BmgT120I GGNCC 4 cut(s) 1213, 1296, 2626, 2917
BmiI GGNNCC 5 cut(s) 198, 966, 1756, 2501, 2820
BmrFI CCNGG 6 cut(s) 360, 408, 771, 1217, 1491, 2675
BmrI ACTGGG 1 cut(s) 2533
BmsI GCATC 7 cut(s) 898, 1195, 1601, 2117, 2347, 2807, 2880
BmuI ACTGGG 1 cut(s) 2533
BpiI GAAGAC 3 cut(s) 2564, 2593, 2736
BplI GAGNNNNNCTC 2 cut(s) 2385, 2417
BpmI CTGGAG 2 cut(s) 1285, 1796
Bpu10I CCTNAGC 1 cut(s) 912
Bpu14I TTCGAA 1 cut(s) 215
BpuEI CTTGAG 1 cut(s) 607
BpuMI CCSGG 2 cut(s) 360, 408
BsaBI GATNNNNATC 2 cut(s) 125, 1498
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 4 cut(s) 1216, 1532, 1817, 2822
BsaWI WCCGGW 2 cut(s) 479, 1797
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bsc4I CCNNNNNNNGG 8 cut(s) 94, 227, 407, 757, 1084, 1357, 1462, 2614
Bse118I RCCGGY 3 cut(s) 284, 433, 1293
Bse1I ACTGG 9 cut(s) 243, 678, 1302, 1320, 1456, 1756, 2441, 2528, 2608
Bse3DI GCAATG 1 cut(s) 1071
Bse8I GATNNNNATC 2 cut(s) 125, 1498
BseAI TCCGGA 1 cut(s) 479
BseBI CCWGG 4 cut(s) 771, 1217, 1491, 2675
BseDI CCNNGG 4 cut(s) 1216, 1532, 1817, 2822
BseGI GGATG 7 cut(s) 706, 1029, 1848, 2108, 2534, 2683, 2798
BseJI GATNNNNATC 2 cut(s) 125, 1498
BseLI CCNNNNNNNGG 8 cut(s) 94, 227, 407, 757, 1084, 1357, 1462, 2614
BseMI GCAATG 1 cut(s) 1071
BseMII CTCAG 3 cut(s) 903, 939, 1653
BseNI ACTGG 9 cut(s) 243, 678, 1302, 1320, 1456, 1756, 2441, 2528, 2608
BseRI GAGGAG 3 cut(s) 361, 1203, 1880
BseSI GKGCMC 1 cut(s) 1174
BseX3I CGGCCG 2 cut(s) 282, 431
BsgI GTGCAG 1 cut(s) 960
Bsh1236I CGCG 5 cut(s) 119, 176, 209, 1541, 2703
Bsh1285I CGRYCG 2 cut(s) 285, 434
BshNI GGYRCC 1 cut(s) 1754
BsiEI CGRYCG 2 cut(s) 285, 434
BsiHKAI GWGCWC 2 cut(s) 1174, 2380
BsiSI CCGG 9 cut(s) 57, 285, 360, 408, 434, 480, 1294, 1798, 2769
BslFI GGGAC 6 cut(s) 168, 441, 1550, 1644, 2316, 2695
BslI CCNNNNNNNGG 8 cut(s) 94, 227, 407, 757, 1084, 1357, 1462, 2614
BsmAI GTCTC 3 cut(s) 357, 408, 2405
BsmBI CGTCTC 2 cut(s) 357, 408
BsmFI GGGAC 6 cut(s) 168, 441, 1550, 1644, 2316, 2695
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 4 cut(s) 88, 514, 1174, 2380
Bsp13I TCCGGA 1 cut(s) 479
Bsp1407I TGTACA 1 cut(s) 780
Bsp19I CCATGG 1 cut(s) 2822
Bsp68I TCGCGA 2 cut(s) 176, 209
BspCNI CTCAG 3 cut(s) 904, 940, 1652
BspEI TCCGGA 1 cut(s) 479
BspFNI CGCG 5 cut(s) 119, 176, 209, 1541, 2703
BspLI GGNNCC 5 cut(s) 198, 966, 1756, 2501, 2820
BspMAI CTGCAG 1 cut(s) 2637
BspMI ACCTGC 1 cut(s) 2637
BspPI GGATC 9 cut(s) 359, 959, 972, 1021, 1122, 1276, 1501, 2813, 2826
BspQI GCTCTTC 1 cut(s) 1020
BspT104I TTCGAA 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 1754
BspTI CTTAAG 2 cut(s) 647, 668
BsrDI GCAATG 1 cut(s) 1071
BsrFI RCCGGY 3 cut(s) 284, 433, 1293
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 9 cut(s) 243, 678, 1302, 1320, 1456, 1756, 2441, 2528, 2608
BssAI RCCGGY 3 cut(s) 284, 433, 1293
BssECI CCNNGG 4 cut(s) 1216, 1532, 1817, 2822
BssNI GRCGYC 1 cut(s) 108
BssSI CACGAG 1 cut(s) 784
BssT1I CCWWGG 3 cut(s) 1532, 1817, 2822
Bst2BI CACGAG 1 cut(s) 784
Bst2UI CCWGG 4 cut(s) 771, 1217, 1491, 2675
Bst4CI ACNGT 7 cut(s) 37, 700, 815, 2003, 2495, 2721, 2735
Bst6I CTCTTC 3 cut(s) 1020, 1077, 2447
BstACI GRCGYC 1 cut(s) 108
BstAFI CTTAAG 2 cut(s) 647, 668
BstAPI GCANNNNNTGC 1 cut(s) 2804
BstAUI TGTACA 1 cut(s) 780
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 7 cut(s) 178, 286, 435, 1011, 1410, 1958, 2211
BstDEI CTNAG 5 cut(s) 912, 948, 1617, 1639, 2459
BstDSI CCRYGG 1 cut(s) 2822
BstEII GGTNACC 1 cut(s) 1733
BstF5I GGATG 7 cut(s) 706, 1029, 1848, 2108, 2534, 2683, 2798
BstFNI CGCG 5 cut(s) 119, 176, 209, 1541, 2703
BstH2I RGCGCY 1 cut(s) 796
BstHHI GCGC 5 cut(s) 795, 1675, 2310, 2673, 2703
BstMAI GTCTC 3 cut(s) 357, 408, 2405
BstMCI CGRYCG 2 cut(s) 285, 434
BstMWI GCNNNNNNNGC 6 cut(s) 234, 1718, 1727, 1829, 2592, 2804
BstNI CCWGG 4 cut(s) 771, 1217, 1491, 2675
BstNSI RCATGY 2 cut(s) 845, 1330
BstPI GGTNACC 1 cut(s) 1733
BstSCI CCNGG 6 cut(s) 358, 406, 769, 1215, 1489, 2673
BstSFI CTRYAG 3 cut(s) 1605, 2633, 2838
BstSLI GKGCMC 1 cut(s) 1174
BstUI CGCG 5 cut(s) 119, 176, 209, 1541, 2703
BstV2I GAAGAC 3 cut(s) 2564, 2593, 2736
BstX2I RGATCY 5 cut(s) 964, 1493, 2197, 2470, 2818
BstYI RGATCY 5 cut(s) 964, 1493, 2197, 2470, 2818
BstZI CGGCCG 2 cut(s) 282, 431
BtgI CCRYGG 1 cut(s) 2822
BtgZI GCGATG 4 cut(s) 267, 1575, 2366, 2852
BtrI CACGTC 2 cut(s) 271, 336
BtsCI GGATG 7 cut(s) 706, 1029, 1848, 2108, 2534, 2683, 2798
BtsIMutI CAGTG 8 cut(s) 705, 1309, 1327, 1463, 1941, 2615, 2740, 2759
BtuMI TCGCGA 2 cut(s) 176, 209
BveI ACCTGC 1 cut(s) 2637
Cac8I GCNNGC 7 cut(s) 178, 286, 435, 1011, 1410, 1958, 2211
CaiI CAGNNNCTG 2 cut(s) 911, 2018
CfoI GCGC 5 cut(s) 795, 1675, 2310, 2673, 2703
Cfr10I RCCGGY 3 cut(s) 284, 433, 1293
Cfr13I GGNCC 4 cut(s) 1213, 1296, 2626, 2917
CseI GACGC 2 cut(s) 1157, 1547
Csp6I GTAC 1 cut(s) 781
CviQI GTAC 1 cut(s) 781
DdeI CTNAG 5 cut(s) 912, 948, 1617, 1639, 2459
DraI TTTAAA 1 cut(s) 2047
EaeI YGGCCR 5 cut(s) 96, 282, 431, 1182, 1453
EagI CGGCCG 2 cut(s) 282, 431
Eam1104I CTCTTC 3 cut(s) 1020, 1077, 2447
EarI CTCTTC 3 cut(s) 1020, 1077, 2447
EciI GGCGGA 3 cut(s) 773, 2431, 2924
EclXI CGGCCG 2 cut(s) 282, 431
Eco130I CCWWGG 3 cut(s) 1532, 1817, 2822
Eco147I AGGCCT 3 cut(s) 738, 1960, 2039
Eco24I GRGCYC 2 cut(s) 88, 514
Eco47I GGWCC 3 cut(s) 1296, 2626, 2917
Eco52I CGGCCG 2 cut(s) 282, 431
Eco57I CTGAAG 3 cut(s) 222, 2587, 2783
Eco91I GGTNACC 1 cut(s) 1733
EcoO109I RGGNCCY 1 cut(s) 2917
EcoO65I GGTNACC 1 cut(s) 1733
EcoRI GAATTC 4 cut(s) 211, 1612, 2239, 2419
EcoRII CCWGG 4 cut(s) 769, 1215, 1489, 2673
EcoT14I CCWWGG 3 cut(s) 1532, 1817, 2822
EcoT22I ATGCAT 2 cut(s) 1052, 2110
EcoT38I GRGCYC 2 cut(s) 88, 514
ErhI CCWWGG 3 cut(s) 1532, 1817, 2822
Esp3I CGTCTC 2 cut(s) 357, 408
FaqI GGGAC 6 cut(s) 168, 441, 1550, 1644, 2316, 2695
FauI CCCGC 3 cut(s) 247, 316, 430
FauNDI CATATG 3 cut(s) 1414, 1579, 2336
FbaI TGATCA 5 cut(s) 1569, 1873, 2068, 2149, 2895
FblI GTMKAC 2 cut(s) 1528, 1792
FokI GGATG 7 cut(s) 693, 1036, 1835, 2095, 2521, 2690, 2785
FriOI GRGCYC 2 cut(s) 88, 514
FspBI CTAG 4 cut(s) 734, 867, 1034, 1199
FspI TGCGCA 1 cut(s) 2309
GlaI GCGC 5 cut(s) 794, 1674, 2309, 2672, 2702
GsuI CTGGAG 2 cut(s) 1285, 1796
HaeII RGCGCY 1 cut(s) 796
HapII CCGG 9 cut(s) 57, 285, 360, 408, 434, 480, 1294, 1798, 2769
HgaI GACGC 2 cut(s) 1157, 1547
HhaI GCGC 5 cut(s) 795, 1675, 2310, 2673, 2703
Hin1I GRCGYC 1 cut(s) 108
Hin6I GCGC 5 cut(s) 793, 1673, 2308, 2671, 2701
HinP1I GCGC 5 cut(s) 793, 1673, 2308, 2671, 2701
HincII GTYRAC 1 cut(s) 1283
HindII GTYRAC 1 cut(s) 1283
HindIII AAGCTT 1 cut(s) 2207
HpaII CCGG 9 cut(s) 57, 285, 360, 408, 434, 480, 1294, 1798, 2769
Hpy99I CGWCG 5 cut(s) 23, 53, 110, 337, 475
HpyCH4III ACNGT 7 cut(s) 37, 700, 815, 2003, 2495, 2721, 2735
HpyCH4IV ACGT 4 cut(s) 108, 270, 335, 662
HpyF10VI GCNNNNNNNGC 6 cut(s) 234, 1718, 1727, 1829, 2592, 2804
HpyF3I CTNAG 5 cut(s) 912, 948, 1617, 1639, 2459
HpySE526I ACGT 4 cut(s) 108, 270, 335, 662
Hsp92I GRCGYC 1 cut(s) 108
HspAI GCGC 5 cut(s) 793, 1673, 2308, 2671, 2701
Kpn2I TCCGGA 1 cut(s) 479
KroI GCCGGC 2 cut(s) 284, 433
KroNI GCCGGC 2 cut(s) 286, 435
Ksp22I TGATCA 5 cut(s) 1569, 1873, 2068, 2149, 2895
LguI GCTCTTC 1 cut(s) 1020
LmnI GCTCC 2 cut(s) 202, 580
LweI GCATC 7 cut(s) 898, 1195, 1601, 2117, 2347, 2807, 2880
MaeI CTAG 4 cut(s) 734, 867, 1034, 1199
MaeII ACGT 4 cut(s) 108, 270, 335, 662
MflI RGATCY 5 cut(s) 964, 1493, 2197, 2470, 2818
MhlI GDGCHC 4 cut(s) 88, 514, 1174, 2380
MlsI TGGCCA 3 cut(s) 98, 1184, 1455
MluNI TGGCCA 3 cut(s) 98, 1184, 1455
MlyI GAGTC 5 cut(s) 1063, 1537, 2179, 2464, 2677
MmeI TCCRAC 4 cut(s) 90, 916, 1054, 2758
Mox20I TGGCCA 3 cut(s) 98, 1184, 1455
Mph1103I ATGCAT 2 cut(s) 1052, 2110
MroI TCCGGA 1 cut(s) 479
MroNI GCCGGC 2 cut(s) 284, 433
MroXI GAANNNNTTC 1 cut(s) 2571
MscI TGGCCA 3 cut(s) 98, 1184, 1455
MseI TTAA 8 cut(s) 549, 648, 669, 1886, 1907, 1922, 2046, 2714
MslI CAYNNNNRTG 3 cut(s) 982, 2103, 2744
Msp20I TGGCCA 3 cut(s) 98, 1184, 1455
MspA1I CMGCKG 1 cut(s) 2837
MspCI CTTAAG 2 cut(s) 647, 668
MspI CCGG 9 cut(s) 57, 285, 360, 408, 434, 480, 1294, 1798, 2769
MspR9I CCNGG 6 cut(s) 360, 408, 771, 1217, 1491, 2675
MvaI CCWGG 4 cut(s) 771, 1217, 1491, 2675
MvnI CGCG 5 cut(s) 119, 176, 209, 1541, 2703
MwoI GCNNNNNNNGC 6 cut(s) 234, 1718, 1727, 1829, 2592, 2804
NaeI GCCGGC 2 cut(s) 286, 435
NciI CCSGG 2 cut(s) 360, 408
NcoI CCATGG 1 cut(s) 2822
NdeI CATATG 3 cut(s) 1414, 1579, 2336
NgoMIV GCCGGC 2 cut(s) 284, 433
NlaIV GGNNCC 5 cut(s) 198, 966, 1756, 2501, 2820
NmeAIII GCCGAG 2 cut(s) 173, 2417
NmuCI GTSAC 9 cut(s) 109, 266, 773, 1145, 1322, 1360, 1733, 2735, 2757
NruI TCGCGA 2 cut(s) 176, 209
NsbI TGCGCA 1 cut(s) 2309
NsiI ATGCAT 2 cut(s) 1052, 2110
NspI RCATGY 2 cut(s) 845, 1330
NspV TTCGAA 1 cut(s) 215
OliI CACNNNNGTG 1 cut(s) 982
PceI AGGCCT 3 cut(s) 738, 1960, 2039
PciI ACATGT 1 cut(s) 1326
PciSI GCTCTTC 1 cut(s) 1020
PcsI WCGNNNNNNNCGW 1 cut(s) 2389
PdiI GCCGGC 2 cut(s) 286, 435
PdmI GAANNNNTTC 1 cut(s) 2571
PfeI GAWTC 7 cut(s) 53, 476, 1194, 1475, 2531, 2689, 2830
PleI GAGTC 5 cut(s) 1063, 1537, 2178, 2463, 2677
PpsI GAGTC 5 cut(s) 1063, 1537, 2178, 2463, 2677
PpuMI RGGWCCY 1 cut(s) 2917
PscI ACATGT 1 cut(s) 1326
Psp5II RGGWCCY 1 cut(s) 2917
Psp6I CCWGG 4 cut(s) 769, 1215, 1489, 2673
PspEI GGTNACC 1 cut(s) 1733
PspGI CCWGG 4 cut(s) 769, 1215, 1489, 2673
PspN4I GGNNCC 5 cut(s) 198, 966, 1756, 2501, 2820
PspPI GGNCC 4 cut(s) 1213, 1296, 2626, 2917
PspPPI RGGWCCY 1 cut(s) 2917
PstI CTGCAG 1 cut(s) 2637
PstNI CAGNNNCTG 2 cut(s) 911, 2018
PsuI RGATCY 5 cut(s) 964, 1493, 2197, 2470, 2818
PvuII CAGCTG 1 cut(s) 2837
RruI TCGCGA 2 cut(s) 176, 209
RsaI GTAC 1 cut(s) 782
RsaNI GTAC 1 cut(s) 781
RseI CAYNNNNRTG 3 cut(s) 982, 2103, 2744
SapI GCTCTTC 1 cut(s) 1020
SaqAI TTAA 8 cut(s) 549, 648, 669, 1886, 1907, 1922, 2046, 2714
Sau96I GGNCC 4 cut(s) 1213, 1296, 2626, 2917
SchI GAGTC 5 cut(s) 1063, 1537, 2179, 2464, 2677
ScrFI CCNGG 6 cut(s) 360, 408, 771, 1217, 1491, 2675
SduI GDGCHC 4 cut(s) 88, 514, 1174, 2380
SfaNI GCATC 7 cut(s) 898, 1195, 1601, 2117, 2347, 2807, 2880
SfcI CTRYAG 3 cut(s) 1605, 2633, 2838
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 3 cut(s) 1296, 2626, 2917
SmiMI CAYNNNNRTG 3 cut(s) 982, 2103, 2744
SmlI CTYRAG 3 cut(s) 586, 647, 668
SmoI CTYRAG 3 cut(s) 586, 647, 668
SseBI AGGCCT 3 cut(s) 738, 1960, 2039
SspI AATATT 2 cut(s) 850, 2192
SspMI CTAG 4 cut(s) 734, 867, 1034, 1199
StuI AGGCCT 3 cut(s) 738, 1960, 2039
StyD4I CCNGG 6 cut(s) 358, 406, 769, 1215, 1489, 2673
StyI CCWWGG 3 cut(s) 1532, 1817, 2822
TaaI ACNGT 7 cut(s) 37, 700, 815, 2003, 2495, 2721, 2735
TaiI ACGT 4 cut(s) 111, 273, 338, 665
TaqI TCGA 6 cut(s) 51, 135, 215, 332, 555, 2173
TatI WGTACW 1 cut(s) 780
TauI GCSGC 3 cut(s) 119, 231, 315
TfiI GAWTC 7 cut(s) 53, 476, 1194, 1475, 2531, 2689, 2830
Tru1I TTAA 8 cut(s) 549, 648, 669, 1886, 1907, 1922, 2046, 2714
Tru9I TTAA 8 cut(s) 549, 648, 669, 1886, 1907, 1922, 2046, 2714
TscAI CASTG 8 cut(s) 705, 1309, 1327, 1463, 1948, 2615, 2740, 2766
TseFI GTSAC 9 cut(s) 109, 266, 773, 1145, 1322, 1360, 1733, 2735, 2757
Tsp45I GTSAC 9 cut(s) 109, 266, 773, 1145, 1322, 1360, 1733, 2735, 2757
TspGWI ACGGA 2 cut(s) 488, 987
TspRI CASTG 8 cut(s) 705, 1309, 1327, 1463, 1948, 2615, 2740, 2766
Vha464I CTTAAG 2 cut(s) 647, 668
VneI GTGCAC 1 cut(s) 1170
VpaK11BI GGWCC 3 cut(s) 1296, 2626, 2917
XapI RAATTY 9 cut(s) 211, 761, 1160, 1598, 1612, 2063, 2239, 2419, 2785
XbaI TCTAGA 1 cut(s) 866
XceI RCATGY 2 cut(s) 845, 1330
XmiI GTMKAC 2 cut(s) 1528, 1792
XmnI GAANNNNTTC 1 cut(s) 2571
XspI CTAG 4 cut(s) 734, 867, 1034, 1199
ZraI GACGTC 1 cut(s) 109
Zsp2I ATGCAT 2 cut(s) 1052, 2110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.