RLG00000022255

SWI SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002288
Physical Location & Seq
Reverse (-)
81961956 .. 81968145
6190 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022255

Sequence Viewer

Length: 3024 bp
ATGGGCAACAAAATCACCGACGAACTATTATCCACCGTTCGTTCAATCGTCGATTCCGGCTACTCCGACATGGACATAATCCGAGCCCTACACATGGCCAACAACGACGTCACCGCCGCGATCAACATCATCTTCGACACCCCCACACCCAAATCTAAAGAAAGGTCAGATTTCGCGAGCAGTCCCAAAATCTCGAGTCCTGAAGTCGCGAATTCGAAGCCGAACGGCGGCGATTGCCCCGCCAGTTGCGGCGATGATGTAGTAGGTGACGTGGAGAGGTCGGCCGGCAGCGAGTGGTGGTTGGTGGGTTGCGGCGAGGTGGCAGGGTTATCGACGTGTAGAGGGAGGAGGATCAAGCCCGGAGACGAAGTGGATTTCACATTTCCTTCGAAGATTTCGCCGTCTCCCGGAAAGGTTTTCGGGAGGGGACGGCCGGCGGCGGCGTGTTCGGAGATTGTGAGGTTCTCCACGAGGGATTCCGGAGAGATTGGACGAATACCTAAGGAATGGGCTCGGTGTCTGTTGCCACTTGTGAGAGATAAGAAGGTTAAAATTGAGGGGCATTGTAAATCTGCTCCTGATGTCTTGAGCATTATGGACACCATTCTCTTGTCTATAAGTGTGTATATCAACAGTTCTATGTTCCTTAAGCAAAAGCAGACCTCGCTTAAGGCAGCCAGTAATTCCACAGAGGAAACAGTGGTTCATCCTCTCCCGACATTGTTCCAGTTGCTAGGCCTAAGTCCTTTCCAGAAGGCAGAATTTACTCCTGGTGACTTGTACACAAGAAAGCGCCCTTTAGACCAAAAGGACAGTTCTGGGCTCTGTGCCTCAATTATACAAGCAAATAAGCATAAAAATCCTTCTAGAAATGAAGATGAGGTTGAAAATGAGGAATGTATTTCGGATGCTGAGGTTGATAACATCATTGGGGTTGGAGATAGCTCTGAGTTAGAGGAAATGGATCCTCCTGCTGCACTTCTGTGTGAACTTCGTCCATACCAAAAGCAGGCTCTTCATTGGATGATCCAACTAGAGAAGGGAAAATGCATGGATGAGGGAGCAATGACTCTTCACCCTTGTTGGGAGGCATATCGTCTTGCAGACAAGAGGGACCGTATTATATATTTGAATGCATTTTCTGGTGACGCAACAACAGAATTTCCAAGCGCACTTCAAATGGCCAGAGGAGGAATCCTAGCTGACGCTATGGGCTTGGGGAAGACGATAATGACAATAGCACTCCTTGTTGCTCATTCAGGACATGGATCATCGGGTAGTCAACCTATGAGCCGGTCCTCTGGTGAAGATATTGAAGCCAGTGACACATCAGACCATCCATCAAAACTCCCAAAGTCGGTCACAAAGTTTTCAGGATTTCATAAGTTGATGAAGCAAAACACGCTTGCATATGGGGGCAGTTTGATTGTATGTCCTATGACCCTTCTTGGCCAGTGGAAGGCAGAGATTGAATCTCATGTGCAACCTGGATCTGTATCAGTATATGTTCATTATGGACAAAGTAGACCAAAGGATGCGACTCTTTTAGCCCAAAGCAATGTTGTGATCACTACATATGGCGTTTTAGCATCAGAATTTTCTACAGAGAATTCTAAGGACAATGGGGGACTTTACTCAGTTAGTTGGTTTAGAGTGGTTCTTGATGAAGCGCACACCATAAAATCCTCTAAAAGCCAAATCTCTATTGCTGCTGCTGCTTTAGTTGCTGGTCGCCGATGGTGTCTTACTGGCACCCCCATACAGATAAATATGTTGTATAATCTAGAGTATTCTCTACATGTTTATTTATATATTATTTTGTGGAATAAACTCATCCAGAAACCCTTTGAGGGGGGTGATGAGAGAGGGTTAAAGCTGGTTCAATCTATCTTAAAGACGATTATGTTAAGGAGAACCAAGTTTAGCACTGATCGTGAAGGCAGGCCCATTTTAGTCCTTCCTCCTGCTGATATTCAGGTGGTTTACTGTGAACTCACAGAAGCTGAAAAAGACTTTTATGAGGCCTTGTTTAAAAGATCAAAGGTGAAATTTGATCAATTTGTTGAGCAAGGACGGGTTCTTCATAATTATGCATCCATATTGGAATTACTTTTACGCCTTCGCCAATGTTGTGATCACCCATTTCTTGTGATGAGTCGAGGTGATACACAAGAATATTCAGATCTGAATAAGCTTGCAAGACGTTTTCTAAAAGGCAGCCAGAATTCTGTGGAAGGGGAAGCCAAAGATTTGCCTTCACGGGCTTATGTTCAGGAAGTTGTTGAAGAAATTCGCAAGGGGGAACAGGGAGAGTGTCCCATATGTCTTGAAGCATTTGAAGATGCAGTATTGACACCTTGTGCTCATCGCTTATGCCGAGAATGTCTCTTGGCAAGTTGGCGGAATTCAACTTCTGGCTTATGTCCAGTTTGCAGGAAGAGTCTAAGTAAGCAAGATCTTATTACAGCACCAACTGTCAGTCGGTTCCATGTTGATATTGAGAAAAACTGGGTGGAATCATCGAAAATAGTTATTCTTTTGCGTGAACTTGAATGTCTTCGTTCGTCAGGCTCAAAAAGCATTGTCTTCAGCCAGTGGACAGCCTTTCTGGACCTCCTGCAGATACCTCTTTCTCGGAGTAACATTCCATTTTTGCGCCTGGATGGGACTCTGAATCAACAACAGCGCGAAAAAGTGTTAAAACAGTTTTCAGAAGACAGTGACATTTTGGTGTTGCTAATGTCACTGAAGGCCGGAGGAGTTGGAATAAATTTAACAGCAGCATCCAATGCCTTTGTCTTGGATCCATGGTGGAATCCAGCTGTAGAGGAACAAGCGGTCATGCGTATTCATCGCATCGGACAAACAAAAAGTGTGATGATCAAACGATTTATCGTGAAGGGAACAGTCGAGGAAAGAATGGAAGCAGTGCAAGCTCGTAAACAGCGGTTAATTTCTGGCGCCTTGACTGACCAAGAAGTTCGCTCGGCTCGCATAGAAGAACTAAAGATGCTTTTTACTTGA

Protein Analysis

1008

Amino Acids

111.75

Weight (kDa)

6.71

Isoelectric Point (pI)

49.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_RAD5A PF24559 5 - 48 5.5e-25 DNA repair protein RAD5A UBA domain
HIRAN PF08797 103 - 206 1.5e-15 HIRAN domain
SNF2-rel_dom PF00176 334 - 715 1.4e-59 SNF2-related domain
DEAD PF00270 404 - 586 3.2e-06 DEAD/DEAH box helicase
zf-C3HC4_3 PF13920 769 - 816 4.2e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 771 - 812 3.7e-08 Ring finger domain
zf-C3HC4_2 PF13923 771 - 811 1.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 772 - 809 3e-09 RING-type zinc-finger
zf-C3HC4 PF00097 772 - 811 5e-09 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 772 - 819 4.7e-06 Prokaryotic RING finger family 4
Helicase_C PF00271 843 - 954 1.6e-20 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 111
AccB1I GGYRCC 2 cut(s) 1751, 2960
AccI GTMKAC 1 cut(s) 1525
AccII CGCG 4 cut(s) 119, 176, 209, 2688
AccIII TCCGGA 1 cut(s) 479
AclWI GGATC 8 cut(s) 359, 959, 972, 1021, 1276, 1498, 2798, 2811
AcoI YGGCCR 5 cut(s) 96, 282, 431, 1182, 1450
AcuI CTGAAG 3 cut(s) 222, 2572, 2768
AcyI GRCGYC 2 cut(s) 108, 2961
AdeI CACNNNGTG 1 cut(s) 2360
AfaI GTAC 1 cut(s) 782
AfiI CCNNNNNNNGG 9 cut(s) 94, 227, 407, 1009, 1084, 1357, 1459, 1850, 1851
AflII CTTAAG 2 cut(s) 647, 668
AflIII ACRYGT 2 cut(s) 335, 1798
AjiI CACGTC 2 cut(s) 271, 336
AjnI CCWGG 3 cut(s) 769, 1486, 2658
AleI CACNNNNGTG 1 cut(s) 982
AluBI AGCT 7 cut(s) 945, 1202, 1876, 2003, 2194, 2822, 2936
AluI AGCT 7 cut(s) 945, 1202, 1876, 2003, 2194, 2822, 2936
Alw21I GWGCWC 1 cut(s) 2365
Alw26I GTCTC 3 cut(s) 357, 408, 2390
AlwI GGATC 8 cut(s) 359, 959, 972, 1021, 1276, 1498, 2798, 2811
AlwNI CAGNNNCTG 1 cut(s) 2003
Ama87I CYCGRG 1 cut(s) 193
Aor13HI TCCGGA 1 cut(s) 479
AoxI GGCC 9 cut(s) 96, 282, 431, 736, 1182, 1450, 1943, 2022, 2751
ApeKI GCWGC 8 cut(s) 288, 674, 974, 1709, 1712, 1715, 2217, 2780
Asp700I GAANNNNTTC 2 cut(s) 2289, 2556
AspLEI GCGC 6 cut(s) 795, 1172, 1672, 2658, 2688, 2963
AspS9I GGNCC 4 cut(s) 1114, 1296, 1944, 2611
AsuC2I CCSGG 2 cut(s) 360, 408
AsuII TTCGAA 2 cut(s) 215, 389
AvaI CYCGRG 1 cut(s) 193
AvaII GGWCC 3 cut(s) 1114, 1296, 2611
AxyI CCTNAGG 1 cut(s) 501
BalI TGGCCA 3 cut(s) 98, 1184, 1452
BamHI GGATCC 2 cut(s) 964, 2803
BanI GGYRCC 2 cut(s) 1751, 2960
BanII GRGCYC 3 cut(s) 88, 514, 825
BauI CACGAG 1 cut(s) 469
BbsI GAAGAC 4 cut(s) 1229, 2549, 2578, 2721
Bbv12I GWGCWC 1 cut(s) 2365
BbvCI CCTCAGC 1 cut(s) 912
BbvI GCAGC 8 cut(s) 300, 686, 961, 1696, 1699, 1702, 2229, 2792
BccI CCATC 4 cut(s) 1344, 1348, 1731, 2657
BceAI ACGGC 3 cut(s) 241, 385, 446
BcgI CGANNNNNNTGC 2 cut(s) 312, 346
BciT130I CCWGG 3 cut(s) 771, 1488, 2660
BclI TGATCA 4 cut(s) 1566, 2053, 2134, 2880
BcnI CCSGG 2 cut(s) 360, 408
BcoDI GTCTC 3 cut(s) 357, 408, 2390
BfaI CTAG 5 cut(s) 734, 867, 1034, 1199, 1784
BfmI CTRYAG 3 cut(s) 1602, 2618, 2823
BfoI RGCGCY 2 cut(s) 796, 2964
BfrI CTTAAG 2 cut(s) 647, 668
BglII AGATCT 2 cut(s) 2182, 2455
Bme1390I CCNGG 5 cut(s) 360, 408, 771, 1488, 2660
Bme18I GGWCC 3 cut(s) 1114, 1296, 2611
BmeT110I CYCGRG 1 cut(s) 193
BmgBI CACGTC 2 cut(s) 271, 336
BmgT120I GGNCC 4 cut(s) 1114, 1296, 1944, 2611
BmiI GGNNCC 6 cut(s) 966, 1115, 1753, 2486, 2805, 2962
BmrFI CCNGG 5 cut(s) 360, 408, 771, 1488, 2660
BmrI ACTGGG 1 cut(s) 2518
BmsI GCATC 8 cut(s) 898, 1525, 1598, 2102, 2332, 2792, 2865, 3000
BmuI ACTGGG 1 cut(s) 2518
BpiI GAAGAC 4 cut(s) 1229, 2549, 2578, 2721
BplI GAGNNNNNCTC 2 cut(s) 2370, 2402
Bpu10I CCTNAGC 1 cut(s) 912
Bpu14I TTCGAA 2 cut(s) 215, 389
BpuEI CTTGAG 1 cut(s) 607
BpuMI CCSGG 2 cut(s) 360, 408
BsaBI GATNNNNATC 2 cut(s) 125, 1495
BsaHI GRCGYC 2 cut(s) 108, 2961
BsaJI CCNNGG 1 cut(s) 2807
BsaWI WCCGGW 1 cut(s) 479
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bsc4I CCNNNNNNNGG 9 cut(s) 94, 227, 407, 1009, 1084, 1357, 1459, 1850, 1851
Bse118I RCCGGY 3 cut(s) 284, 433, 1293
Bse1I ACTGG 9 cut(s) 243, 678, 727, 1320, 1453, 1753, 2426, 2513, 2593
Bse21I CCTNAGG 1 cut(s) 501
Bse3DI GCAATG 2 cut(s) 1071, 1564
Bse8I GATNNNNATC 2 cut(s) 125, 1495
BseAI TCCGGA 1 cut(s) 479
BseBI CCWGG 3 cut(s) 771, 1488, 2660
BseDI CCNNGG 1 cut(s) 2807
BseJI GATNNNNATC 2 cut(s) 125, 1495
BseLI CCNNNNNNNGG 9 cut(s) 94, 227, 407, 1009, 1084, 1357, 1459, 1850, 1851
BseMI GCAATG 2 cut(s) 1071, 1564
BseMII CTCAG 3 cut(s) 903, 939, 1650
BseNI ACTGG 9 cut(s) 243, 678, 727, 1320, 1453, 1753, 2426, 2513, 2593
BseRI GAGGAG 3 cut(s) 361, 1203, 2772
BseX3I CGGCCG 2 cut(s) 282, 431
BseXI GCAGC 8 cut(s) 300, 686, 961, 1696, 1699, 1702, 2229, 2792
BsgI GTGCAG 1 cut(s) 960
Bsh1236I CGCG 4 cut(s) 119, 176, 209, 2688
Bsh1285I CGRYCG 2 cut(s) 285, 434
BshFI GGCC 9 cut(s) 98, 284, 433, 738, 1184, 1452, 1945, 2024, 2753
BshNI GGYRCC 2 cut(s) 1751, 2960
BsiEI CGRYCG 2 cut(s) 285, 434
BsiHKAI GWGCWC 1 cut(s) 2365
BsiHKCI CYCGRG 1 cut(s) 193
BsiSI CCGG 8 cut(s) 57, 285, 360, 408, 434, 480, 1294, 2754
BslFI GGGAC 6 cut(s) 168, 441, 1127, 1641, 2301, 2680
BslI CCNNNNNNNGG 9 cut(s) 94, 227, 407, 1009, 1084, 1357, 1459, 1850, 1851
BsmAI GTCTC 3 cut(s) 357, 408, 2390
BsmBI CGTCTC 2 cut(s) 357, 408
BsmFI GGGAC 6 cut(s) 168, 441, 1127, 1641, 2301, 2680
BsmI GAATGC 1 cut(s) 1138
BsnI GGCC 9 cut(s) 98, 284, 433, 738, 1184, 1452, 1945, 2024, 2753
BsoBI CYCGRG 1 cut(s) 193
Bsp119I TTCGAA 2 cut(s) 215, 389
Bsp1286I GDGCHC 4 cut(s) 88, 514, 825, 2365
Bsp13I TCCGGA 1 cut(s) 479
Bsp1407I TGTACA 1 cut(s) 780
Bsp19I CCATGG 1 cut(s) 2807
Bsp68I TCGCGA 2 cut(s) 176, 209
BspANI GGCC 9 cut(s) 98, 284, 433, 738, 1184, 1452, 1945, 2024, 2753
BspCNI CTCAG 3 cut(s) 904, 940, 1649
BspEI TCCGGA 1 cut(s) 479
BspFNI CGCG 4 cut(s) 119, 176, 209, 2688
BspLI GGNNCC 6 cut(s) 966, 1115, 1753, 2486, 2805, 2962
BspMAI CTGCAG 1 cut(s) 2622
BspPI GGATC 8 cut(s) 359, 959, 972, 1021, 1276, 1498, 2798, 2811
BspQI GCTCTTC 1 cut(s) 1020
BspT104I TTCGAA 2 cut(s) 215, 389
BspT107I GGYRCC 2 cut(s) 1751, 2960
BspTI CTTAAG 2 cut(s) 647, 668
BsrDI GCAATG 2 cut(s) 1071, 1564
BsrFI RCCGGY 3 cut(s) 284, 433, 1293
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 9 cut(s) 243, 678, 727, 1320, 1453, 1753, 2426, 2513, 2593
BssAI RCCGGY 3 cut(s) 284, 433, 1293
BssECI CCNNGG 1 cut(s) 2807
BssNI GRCGYC 2 cut(s) 108, 2961
BssSI CACGAG 1 cut(s) 469
BssT1I CCWWGG 1 cut(s) 2807
Bst2BI CACGAG 1 cut(s) 469
Bst2UI CCWGG 3 cut(s) 771, 1488, 2660
Bst6I CTCTTC 3 cut(s) 1020, 1077, 2432
BstACI GRCGYC 2 cut(s) 108, 2961
BstAFI CTTAAG 2 cut(s) 647, 668
BstAPI GCANNNNNTGC 1 cut(s) 2789
BstAUI TGTACA 1 cut(s) 780
BstBI TTCGAA 2 cut(s) 215, 389
BstC8I GCNNGC 9 cut(s) 178, 286, 435, 1011, 1407, 1943, 2196, 2934, 2992
BstDEI CTNAG 7 cut(s) 501, 740, 912, 948, 1614, 1636, 2444
BstDSI CCRYGG 1 cut(s) 2807
BstFNI CGCG 4 cut(s) 119, 176, 209, 2688
BstH2I RGCGCY 2 cut(s) 796, 2964
BstHHI GCGC 6 cut(s) 795, 1172, 1672, 2658, 2688, 2963
BstMAI GTCTC 3 cut(s) 357, 408, 2390
BstMCI CGRYCG 2 cut(s) 285, 434
BstMWI GCNNNNNNNGC 9 cut(s) 234, 664, 1402, 1715, 1724, 2577, 2789, 2933, 2991
BstNI CCWGG 3 cut(s) 771, 1488, 2660
BstNSI RCATGY 1 cut(s) 1802
BstSCI CCNGG 5 cut(s) 358, 406, 769, 1486, 2658
BstSFI CTRYAG 3 cut(s) 1602, 2618, 2823
BstUI CGCG 4 cut(s) 119, 176, 209, 2688
BstV1I GCAGC 8 cut(s) 300, 686, 961, 1696, 1699, 1702, 2229, 2792
BstV2I GAAGAC 4 cut(s) 1229, 2549, 2578, 2721
BstX2I RGATCY 5 cut(s) 964, 1490, 2182, 2455, 2803
BstYI RGATCY 5 cut(s) 964, 1490, 2182, 2455, 2803
BstZI CGGCCG 2 cut(s) 282, 431
Bsu36I CCTNAGG 1 cut(s) 501
BsuRI GGCC 9 cut(s) 98, 284, 433, 738, 1184, 1452, 1945, 2024, 2753
BtgI CCRYGG 1 cut(s) 2807
BtgZI GCGATG 3 cut(s) 267, 2351, 2837
BtrI CACGTC 2 cut(s) 271, 336
BtsI GCAGTG 1 cut(s) 2934
BtsIMutI CAGTG 8 cut(s) 705, 1327, 1460, 1926, 2600, 2725, 2744, 2934
BtuMI TCGCGA 2 cut(s) 176, 209
Cac8I GCNNGC 9 cut(s) 178, 286, 435, 1011, 1407, 1943, 2196, 2934, 2992
CaiI CAGNNNCTG 1 cut(s) 2003
CfoI GCGC 6 cut(s) 795, 1172, 1672, 2658, 2688, 2963
Cfr10I RCCGGY 3 cut(s) 284, 433, 1293
Cfr13I GGNCC 4 cut(s) 1114, 1296, 1944, 2611
CseI GACGC 2 cut(s) 1157, 1214
Csp6I GTAC 1 cut(s) 781
CviAII CATG 9 cut(s) 70, 94, 1051, 1265, 1478, 1799, 2489, 2808, 2842
CviQI GTAC 1 cut(s) 781
DdeI CTNAG 7 cut(s) 501, 740, 912, 948, 1614, 1636, 2444
DinI GGCGCC 1 cut(s) 2962
DraI TTTAAA 1 cut(s) 2032
DraIII CACNNNGTG 1 cut(s) 2360
EaeI YGGCCR 5 cut(s) 96, 282, 431, 1182, 1450
EagI CGGCCG 2 cut(s) 282, 431
Eam1104I CTCTTC 3 cut(s) 1020, 1077, 2432
EarI CTCTTC 3 cut(s) 1020, 1077, 2432
EciI GGCGGA 1 cut(s) 2416
EclXI CGGCCG 2 cut(s) 282, 431
Eco130I CCWWGG 1 cut(s) 2807
Eco147I AGGCCT 2 cut(s) 738, 2024
Eco24I GRGCYC 3 cut(s) 88, 514, 825
Eco47I GGWCC 3 cut(s) 1114, 1296, 2611
Eco52I CGGCCG 2 cut(s) 282, 431
Eco57I CTGAAG 3 cut(s) 222, 2572, 2768
Eco81I CCTNAGG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 193
EcoRI GAATTC 4 cut(s) 211, 1609, 2224, 2404
EcoRII CCWGG 3 cut(s) 769, 1486, 2658
EcoT14I CCWWGG 1 cut(s) 2807
EcoT22I ATGCAT 3 cut(s) 1052, 1138, 2095
EcoT38I GRGCYC 3 cut(s) 88, 514, 825
EgeI GGCGCC 1 cut(s) 2962
EheI GGCGCC 1 cut(s) 2962
ErhI CCWWGG 1 cut(s) 2807
Esp3I CGTCTC 2 cut(s) 357, 408
FaeI CATG 9 cut(s) 73, 97, 1054, 1268, 1481, 1802, 2492, 2811, 2845
FaqI GGGAC 6 cut(s) 168, 441, 1127, 1641, 2301, 2680
FatI CATG 9 cut(s) 69, 93, 1050, 1264, 1477, 1798, 2488, 2807, 2841
FauI CCCGC 1 cut(s) 247
FauNDI CATATG 3 cut(s) 1411, 1576, 2321
FbaI TGATCA 4 cut(s) 1566, 2053, 2134, 2880
FblI GTMKAC 1 cut(s) 1525
FriOI GRGCYC 3 cut(s) 88, 514, 825
FspBI CTAG 5 cut(s) 734, 867, 1034, 1199, 1784
GlaI GCGC 6 cut(s) 794, 1171, 1671, 2657, 2687, 2962
HaeII RGCGCY 2 cut(s) 796, 2964
HaeIII GGCC 9 cut(s) 98, 284, 433, 738, 1184, 1452, 1945, 2024, 2753
HapII CCGG 8 cut(s) 57, 285, 360, 408, 434, 480, 1294, 2754
HgaI GACGC 2 cut(s) 1157, 1214
HhaI GCGC 6 cut(s) 795, 1172, 1672, 2658, 2688, 2963
Hin1I GRCGYC 2 cut(s) 108, 2961
Hin1II CATG 9 cut(s) 73, 97, 1054, 1268, 1481, 1802, 2492, 2811, 2845
Hin6I GCGC 6 cut(s) 793, 1170, 1670, 2656, 2686, 2961
HinP1I GCGC 6 cut(s) 793, 1170, 1670, 2656, 2686, 2961
HincII GTYRAC 1 cut(s) 1283
HindII GTYRAC 1 cut(s) 1283
HindIII AAGCTT 1 cut(s) 2192
HpaII CCGG 8 cut(s) 57, 285, 360, 408, 434, 480, 1294, 2754
Hpy166II GTNNAC 9 cut(s) 783, 989, 1283, 1526, 1984, 1991, 2546, 2598, 2942
Hpy8I GTNNAC 9 cut(s) 783, 989, 1283, 1526, 1984, 1991, 2546, 2598, 2942
Hpy99I CGWCG 4 cut(s) 23, 53, 110, 337
HpyCH4IV ACGT 4 cut(s) 108, 270, 335, 2203
HpyF10VI GCNNNNNNNGC 9 cut(s) 234, 664, 1402, 1715, 1724, 2577, 2789, 2933, 2991
HpyF3I CTNAG 7 cut(s) 501, 740, 912, 948, 1614, 1636, 2444
HpySE526I ACGT 4 cut(s) 108, 270, 335, 2203
Hsp92I GRCGYC 2 cut(s) 108, 2961
Hsp92II CATG 9 cut(s) 73, 97, 1054, 1268, 1481, 1802, 2492, 2811, 2845
HspAI GCGC 6 cut(s) 793, 1170, 1670, 2656, 2686, 2961
KasI GGCGCC 1 cut(s) 2960
Kpn2I TCCGGA 1 cut(s) 479
KroI GCCGGC 2 cut(s) 284, 433
KroNI GCCGGC 2 cut(s) 286, 435
Ksp22I TGATCA 4 cut(s) 1566, 2053, 2134, 2880
LguI GCTCTTC 1 cut(s) 1020
LmnI GCTCC 2 cut(s) 580, 1061
Lsp1109I GCAGC 8 cut(s) 300, 686, 961, 1696, 1699, 1702, 2229, 2792
LweI GCATC 8 cut(s) 898, 1525, 1598, 2102, 2332, 2792, 2865, 3000
MaeI CTAG 5 cut(s) 734, 867, 1034, 1199, 1784
MaeII ACGT 4 cut(s) 108, 270, 335, 2203
MaeIII GTNAC 9 cut(s) 109, 266, 773, 1145, 1322, 1360, 2639, 2720, 2742
MflI RGATCY 5 cut(s) 964, 1490, 2182, 2455, 2803
MhlI GDGCHC 4 cut(s) 88, 514, 825, 2365
MlsI TGGCCA 3 cut(s) 98, 1184, 1452
MluNI TGGCCA 3 cut(s) 98, 1184, 1452
Mly113I GGCGCC 1 cut(s) 2961
MlyI GAGTC 6 cut(s) 205, 1063, 1534, 2164, 2449, 2662
MmeI TCCRAC 4 cut(s) 90, 916, 1054, 2743
Mox20I TGGCCA 3 cut(s) 98, 1184, 1452
Mph1103I ATGCAT 3 cut(s) 1052, 1138, 2095
MroI TCCGGA 1 cut(s) 479
MroNI GCCGGC 2 cut(s) 284, 433
MroXI GAANNNNTTC 2 cut(s) 2289, 2556
MscI TGGCCA 3 cut(s) 98, 1184, 1452
MslI CAYNNNNRTG 3 cut(s) 982, 2088, 2729
Msp20I TGGCCA 3 cut(s) 98, 1184, 1452
MspA1I CMGCKG 2 cut(s) 2822, 2947
MspCI CTTAAG 2 cut(s) 647, 668
MspI CCGG 8 cut(s) 57, 285, 360, 408, 434, 480, 1294, 2754
MspR9I CCNGG 5 cut(s) 360, 408, 771, 1488, 2660
Mva1269I GAATGC 1 cut(s) 1138
MvaI CCWGG 3 cut(s) 771, 1488, 2660
MvnI CGCG 4 cut(s) 119, 176, 209, 2688
MwoI GCNNNNNNNGC 9 cut(s) 234, 664, 1402, 1715, 1724, 2577, 2789, 2933, 2991
NaeI GCCGGC 2 cut(s) 286, 435
NarI GGCGCC 1 cut(s) 2961
NciI CCSGG 2 cut(s) 360, 408
NcoI CCATGG 1 cut(s) 2807
NdeI CATATG 3 cut(s) 1411, 1576, 2321
NgoMIV GCCGGC 2 cut(s) 284, 433
NlaIII CATG 9 cut(s) 73, 97, 1054, 1268, 1481, 1802, 2492, 2811, 2845
NlaIV GGNNCC 6 cut(s) 966, 1115, 1753, 2486, 2805, 2962
NmeAIII GCCGAG 2 cut(s) 2402, 2966
NmuCI GTSAC 8 cut(s) 109, 266, 773, 1145, 1322, 1360, 2720, 2742
NruI TCGCGA 2 cut(s) 176, 209
NsiI ATGCAT 3 cut(s) 1052, 1138, 2095
NspI RCATGY 1 cut(s) 1802
NspV TTCGAA 2 cut(s) 215, 389
OliI CACNNNNGTG 1 cut(s) 982
PaeR7I CTCGAG 1 cut(s) 193
PceI AGGCCT 2 cut(s) 738, 2024
PciI ACATGT 1 cut(s) 1798
PciSI GCTCTTC 1 cut(s) 1020
PcsI WCGNNNNNNNCGW 1 cut(s) 2374
PctI GAATGC 1 cut(s) 1138
PdiI GCCGGC 2 cut(s) 286, 435
PdmI GAANNNNTTC 2 cut(s) 2289, 2556
PfeI GAWTC 7 cut(s) 53, 476, 1194, 1472, 2516, 2674, 2815
PfoI TCCNGGA 1 cut(s) 406
PleI GAGTC 6 cut(s) 204, 1063, 1534, 2163, 2448, 2662
PluTI GGCGCC 1 cut(s) 2964
PpsI GAGTC 6 cut(s) 204, 1063, 1534, 2163, 2448, 2662
PscI ACATGT 1 cut(s) 1798
Psp6I CCWGG 3 cut(s) 769, 1486, 2658
PspGI CCWGG 3 cut(s) 769, 1486, 2658
PspN4I GGNNCC 6 cut(s) 966, 1115, 1753, 2486, 2805, 2962
PspPI GGNCC 4 cut(s) 1114, 1296, 1944, 2611
PstI CTGCAG 1 cut(s) 2622
PstNI CAGNNNCTG 1 cut(s) 2003
PsuI RGATCY 5 cut(s) 964, 1490, 2182, 2455, 2803
PvuII CAGCTG 1 cut(s) 2822
RruI TCGCGA 2 cut(s) 176, 209
RsaI GTAC 1 cut(s) 782
RsaNI GTAC 1 cut(s) 781
RseI CAYNNNNRTG 3 cut(s) 982, 2088, 2729
SapI GCTCTTC 1 cut(s) 1020
Sau96I GGNCC 4 cut(s) 1114, 1296, 1944, 2611
SchI GAGTC 6 cut(s) 205, 1063, 1534, 2164, 2449, 2662
ScrFI CCNGG 5 cut(s) 360, 408, 771, 1488, 2660
SduI GDGCHC 4 cut(s) 88, 514, 825, 2365
SfaNI GCATC 8 cut(s) 898, 1525, 1598, 2102, 2332, 2792, 2865, 3000
SfcI CTRYAG 3 cut(s) 1602, 2618, 2823
SfoI GGCGCC 1 cut(s) 2962
Sfr274I CTCGAG 1 cut(s) 193
SfuI TTCGAA 2 cut(s) 215, 389
SinI GGWCC 3 cut(s) 1114, 1296, 2611
SlaI CTCGAG 1 cut(s) 193
SmiMI CAYNNNNRTG 3 cut(s) 982, 2088, 2729
SmlI CTYRAG 4 cut(s) 193, 586, 647, 668
SmoI CTYRAG 4 cut(s) 193, 586, 647, 668
SseBI AGGCCT 2 cut(s) 738, 2024
SspDI GGCGCC 1 cut(s) 2960
SspI AATATT 1 cut(s) 2177
SspMI CTAG 5 cut(s) 734, 867, 1034, 1199, 1784
StuI AGGCCT 2 cut(s) 738, 2024
StyD4I CCNGG 5 cut(s) 358, 406, 769, 1486, 2658
StyI CCWWGG 1 cut(s) 2807
TaiI ACGT 4 cut(s) 111, 273, 338, 2206
TaqI TCGA 9 cut(s) 51, 135, 194, 215, 332, 389, 2158, 2522, 2910
TaqII GACCGA 1 cut(s) 1348
TatI WGTACW 1 cut(s) 780
TauI GCSGC 6 cut(s) 119, 231, 252, 315, 440, 443
TfiI GAWTC 7 cut(s) 53, 476, 1194, 1472, 2516, 2674, 2815
TscAI CASTG 8 cut(s) 705, 1327, 1460, 1933, 2600, 2725, 2751, 2934
TseFI GTSAC 8 cut(s) 109, 266, 773, 1145, 1322, 1360, 2720, 2742
TseI GCWGC 8 cut(s) 288, 674, 974, 1709, 1712, 1715, 2217, 2780
Tsp45I GTSAC 8 cut(s) 109, 266, 773, 1145, 1322, 1360, 2720, 2742
TspDTI ATGAA 9 cut(s) 695, 888, 1007, 1370, 1406, 1499, 1680, 2072, 2840
TspRI CASTG 8 cut(s) 705, 1327, 1460, 1933, 2600, 2725, 2751, 2934
Vha464I CTTAAG 2 cut(s) 647, 668
VpaK11BI GGWCC 3 cut(s) 1114, 1296, 2611
XbaI TCTAGA 2 cut(s) 866, 1783
XceI RCATGY 1 cut(s) 1802
XhoI CTCGAG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 1525
XmnI GAANNNNTTC 2 cut(s) 2289, 2556
XspI CTAG 5 cut(s) 734, 867, 1034, 1199, 1784
ZraI GACGTC 1 cut(s) 109
Zsp2I ATGCAT 3 cut(s) 1052, 1138, 2095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.