Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0002288

Physical Location & Seq

Genomic Coordinates Forward (+)

54757257 .. 54760168

Gene Structure 2912 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0376700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2232 bp

ATGTCTGGCTCCTCAAACCACTACTGTAACCTCCTCAAGCTCTGCTGCCAATCCGCAAACCACCCACAAGCAAAGAAAATCCACTCCCACATTATCAGAACCTTGCCGAACCCCGAGACCTTCCTCCTCAACAATCTCATTACCGCCTACGGCAAACTCGGCAACTTAACATACGCCCGCCATGTGTTCGATCAAATGCCTCACCCAACTCTTTTCTCATGGAACTCCATTCTCTCCGTCTATTCCAAATCCCGCCGTGTTTCCGAGATGCAAGACGTCTTTAATCGGATGCCGAGCCGTGATGGCGTGTCCTGGTGTTCGTTTATTGCTGGGTACGCCGGGTGTGGCTTGGTTGCCGAGGCCGTTAGGGTTTATGGTTTGATGTTGAGGGACGGAGCTGGTAATTTGACCCGGATTACGTTTTCCACACTGCTTGTGCTGACTTCGAATCGCGGGTGTGAGAAGCTGGGGAGACAGTTTCATGGGCACATAGTGAAGTTTGGGTTTGAGTCGTATGTGTTTGTTGGCAGTCCTTTGATTGATATGTACTCGAAAGCGGGGTTGCTTCTTGATGCAAAGCGGGTTTTTGAGTTGATGCCGGAGAGGAATGTGGTTATGTACAATACACTTATTACTGGCCTTTTGAGGTGTGGATTGATCGGAGATGCTGAGTGTTTGTTTGGTCACATGCCTGAAAAAGACTCCATTTCGTGGACCACAATGATTACCGGACTCACGAGAAATGGTGCAGGCAGAAAAGCACTTGATAAGTTTAGAGAAATGAGGTCGGAAGGTTTGGGGATGGATCAATATACGTTTGGTAGCGCATTGACTGCGTGTGGGGGACTGTTGGCTCTGCAGGAGGGCAAGCAAGTTCATGCTTGTATTGTTCGGACTGATTTTATGGATAATATCTTCGTCAGTAGCGCTCTTGTTGACATGTATTGTAAGTGTGGAAGCATAAAAGCTGCAGAGACAGTTTTCAGGAGAATGAGCTGCAAGAATGTAGTATCGTGGACTGCCTTGCTTGTTGGTTATGGCCAGAATGGGTACAGTGAAGAAGCTGTTAGAGTTTTTTGTGATATGCAGAGAAGTGGGGTTGAACCAGATGAATATACCCTGGGAAGCTTAATTAGCTCGTGTGCTAACTTAGCAAGCTTAGAAGAAGGTGCCCAGTTCCATGGTCAAGCTCTAGTTTCAGGCCTGATTTCTTTCACTACAGTTTCAAATGCACTCGTTACCTTATACGGTAAGTGTGGAAGTATCGAAGACTCCCATAGGCTGTTTGATGAGATGAACATCCGGGATGAAGTCTCCTGGTCAGCATTGGTTTCAGGGTATGCCCGGTTTGGAAAAGCCAATGAGACAATTGCTTTGTTTGAGAGGATGTTGGCCCAGGGGTTAAAACCTGATGGAGTTACTTTCATAGGGGTTCTTTCAGCCTGTAGTAGAGCAGGACTAGTAGAGAGAGGACATCAGTATTTTGAATCAATGACTAAAGTACATGGAATTACACCGATTCTTGATCACTACACTTGCATCATTGACCTGCTCAGCCGAGCAGGAAGATTAGAAGAAGCAAGAAGCTTCATTAATAAGATGCCTATGCGTCCTGATGCAATTGGTTGGGCCACCTTGCTAAGCTCATGTAGATTTCGCTCTAATATGGAGATTGGAAAATGGGCAGCTAAGTCTCTTCGAGAAATAGATCCCCAGAATCCTGCAAGCTATATCTTGCTCTCAAGCATCTATGCTGCTAAAGGGAAGTGGGATGAAGTGTCTAAATTGAGGACAGGAATGAGAGATAAGGGGATCAGAAAGGAACCAGGATGCAGTTGGATTAAATATAAGAGCAGAGTACACATCTTTTCAGCAGATGACCAGTCAAGTCCATTTTCAGATCAGATATACGCTGAGCTGGAGAAACTATATTCTAAAATGATAGAAGAAGGATATGAGCCAGATGTGAGTTCAGTTCTCCATGATGTTGACGAATCACAAAAGATGAAGATGCTTAATTACCACAGTGAGAGGCTTGCAATTGCCTTTGGATTGATATTTATTCCTTCTGGCCTTCCTATTCGAGTTGTTAAAAACCTTAGAGTATGTGGGGACTGCCACAATGCCACTAAATATATTTCCAAGATCACCAAGAGAGAGATACTTGTAAGAGATGCTGTCCGCTACCACTTATTTAAAGACGGAACTTGTTCATGTGGAGATTTTTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009987 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044764 GO:0045003 GO:0046483 GO:0050896 GO:0051704 GO:0051716 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HIRAN Helicase_C SNF2_N zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

743

Amino Acids

82.87

Weight (kDa)

8.58

Isoelectric Point (pI)

44.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	234 - 280	1.5e-08	PPR repeat family
PPR	PF01535	236 - 266	7.3e-06	PPR repeat
PPR_2	PF13041	334 - 383	8.1e-14	PPR repeat family
PPR	PF01535	337 - 367	1.4e-07	PPR repeat
PPR_2	PF13041	436 - 483	1.7e-07	PPR repeat family
E_motif	PF20431	554 - 615	1.1e-22	E motif
DYW_deaminase	PF14432	651 - 743	9.2e-41	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22750
fragaria_vesca	FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660
malus_domestica	MD17G1018000.v1.1
prunus_persica	Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1
pyrus_communis	pycom17g01450
rosa_chinensis	RchiOBHm_Chr2g0174191 RchiOBHm_Chr4g0410751
rosa_laevigata	RLG00000022255 RLG00000022256 RLG00000029588
rosa_multiflora	Rmu_sc0000954.1_g000002 Rmu_ssc0000198.1_g000002
rosa_roxburghii	Rroxscaffold_2G00078340 Rroxscaffold_2G00078370 Rroxscaffold_5G00355500
rosa_rugosa	Rorug02G0575800 Rorug02G0575900 Rorug02G0576000 Rorug04G0376700
rosa_samantha	Rh2AG655200 Rh2BG666400 Rh2CG630600 Rh2DG681000 Rh4AG162800 Rh4BG161900 Rh4BG162000 Rh4DG158500 Rh7CG048600
rosa_wichuraiana	Rw2G053780 Rw4G013570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	279
Acc36I	ACCTGC	1 cut(s)	1557
AccB1I	GGYRCC	1 cut(s)	1169
AccB7I	CCANNNNNTGG	1 cut(s)	711
AccII	CGCG	1 cut(s)	453
AciI	CCGC	8 cut(s)	54, 144, 178, 253, 453, 557, 580, 2182
AclWI	GGATC	3 cut(s)	813, 1703, 1820
AcoI	YGGCCR	1 cut(s)	1039
AcyI	GRCGYC	1 cut(s)	276
AdeI	CACNNNGTG	1 cut(s)	493
AfaI	GTAC	6 cut(s)	335, 548, 620, 1052, 1503, 1860
AfeI	AGCGCT	1 cut(s)	928
AfiI	CCNNNNNNNGG	1 cut(s)	711
AflIII	ACRYGT	1 cut(s)	939
AgsI	TTSAA	3 cut(s)	1103, 1227, 1487
AhlI	ACTAGT	1 cut(s)	1459
AjnI	CCWGG	5 cut(s)	311, 1119, 1316, 1395, 1825
Alw26I	GTCTC	6 cut(s)	110, 466, 968, 1318, 1358, 1698
AlwI	GGATC	3 cut(s)	813, 1703, 1820
Ama87I	CYCGRG	1 cut(s)	113
Aor51HI	AGCGCT	1 cut(s)	928
AoxI	GGCC	7 cut(s)	360, 637, 1039, 1201, 1392, 1629, 2071
ApeKI	GCWGC	5 cut(s)	45, 968, 996, 1685, 1754
ArsI	GACNNNNNNTTYG	2 cut(s)	1357, 1389
AseI	ATTAAT	1 cut(s)	1593
Asp700I	GAANNNNTTC	1 cut(s)	2209
AspLEI	GCGC	2 cut(s)	827, 929
AspS9I	GGNCC	3 cut(s)	714, 1393, 1629
AsuC2I	CCSGG	4 cut(s)	340, 412, 1304, 1345
AsuHPI	GGTGA	2 cut(s)	194, 2140
AsuII	TTCGAA	1 cut(s)	446
AvaI	CYCGRG	1 cut(s)	113
AvaII	GGWCC	1 cut(s)	714
BaeGI	GKGCMC	2 cut(s)	489, 1174
BalI	TGGCCA	1 cut(s)	1041
BanI	GGYRCC	1 cut(s)	1169
BauI	CACGAG	2 cut(s)	736, 1138
BbsI	GAAGAC	1 cut(s)	1275
BbvI	GCAGC	5 cut(s)	32, 955, 983, 1697, 1741
BccI	CCATC	3 cut(s)	296, 796, 1406
BceAI	ACGGC	4 cut(s)	166, 240, 282, 347
BciT130I	CCWGG	5 cut(s)	313, 1121, 1318, 1397, 1827
BclI	TGATCA	1 cut(s)	1525
BcnI	CCSGG	4 cut(s)	340, 412, 1304, 1345
BcoDI	GTCTC	6 cut(s)	110, 466, 968, 1318, 1358, 1698
BcuI	ACTAGT	1 cut(s)	1459
BfaI	CTAG	2 cut(s)	1193, 1460
BfmI	CTRYAG	4 cut(s)	857, 969, 1218, 1444
BfoI	RGCGCY	1 cut(s)	930
BfuAI	ACCTGC	1 cut(s)	1557
BglI	GCCNNNNNGGC	1 cut(s)	303
BisI	GCNGC	5 cut(s)	46, 969, 997, 1686, 1755
BlpI	GCTNAGC	3 cut(s)	1553, 1640, 1914
BlsI	GCNGC	5 cut(s)	47, 970, 998, 1687, 1756
Bme1390I	CCNGG	9 cut(s)	313, 340, 412, 1121, 1304, 1318, 1345, 1397, 1827
Bme18I	GGWCC	1 cut(s)	714
BmeT110I	CYCGRG	1 cut(s)	113
BmgT120I	GGNCC	3 cut(s)	714, 1393, 1629
BmiI	GGNNCC	3 cut(s)	10, 1171, 1824
BmrFI	CCNGG	9 cut(s)	313, 340, 412, 1121, 1304, 1318, 1345, 1397, 1827
BmrI	ACTGGG	1 cut(s)	1168
BmuI	ACTGGG	1 cut(s)	1168
BpiI	GAAGAC	1 cut(s)	1275
BpmI	CTGGAG	1 cut(s)	1940
Bpu1102I	GCTNAGC	3 cut(s)	1553, 1640, 1914
Bpu14I	TTCGAA	1 cut(s)	446
BpuEI	CTTGAG	2 cut(s)	20, 1726
BpuMI	CCSGG	4 cut(s)	340, 412, 1304, 1345
BsaBI	GATNNNNATC	1 cut(s)	1298
BsaHI	GRCGYC	1 cut(s)	276
BsaI	GGTCTC	1 cut(s)	110
BsaJI	CCNNGG	6 cut(s)	357, 1119, 1120, 1180, 1395, 1396
BsaWI	WCCGGW	1 cut(s)	728
Bsc4I	CCNNNNNNNGG	1 cut(s)	711
Bse1I	ACTGG	3 cut(s)	640, 1174, 1882
Bse8I	GATNNNNATC	1 cut(s)	1298
BseBI	CCWGG	5 cut(s)	313, 1121, 1318, 1397, 1827
BseDI	CCNNGG	6 cut(s)	357, 1119, 1120, 1180, 1395, 1396
BseGI	GGATG	7 cut(s)	294, 807, 1299, 1312, 1392, 1777, 1835
BseJI	GATNNNNATC	1 cut(s)	1298
BseLI	CCNNNNNNNGG	1 cut(s)	711
BseMII	CTCAG	3 cut(s)	660, 1567, 1905
BseNI	ACTGG	3 cut(s)	640, 1174, 1882
BseRI	GAGGAG	2 cut(s)	23, 116
BseSI	GKGCMC	2 cut(s)	489, 1174
BseXI	GCAGC	5 cut(s)	32, 955, 983, 1697, 1741
BseYI	CCCAGC	2 cut(s)	329, 466
BsgI	GTGCAG	1 cut(s)	768
Bsh1236I	CGCG	1 cut(s)	453
BshFI	GGCC	7 cut(s)	362, 639, 1041, 1203, 1394, 1631, 2073
BshNI	GGYRCC	1 cut(s)	1169
BsiHKCI	CYCGRG	1 cut(s)	113
BsiSI	CCGG	6 cut(s)	339, 412, 599, 729, 1303, 1345
BslFI	GGGAC	3 cut(s)	404, 858, 2126
BslI	CCNNNNNNNGG	1 cut(s)	711
BsmAI	GTCTC	6 cut(s)	110, 466, 968, 1318, 1358, 1698
BsmFI	GGGAC	3 cut(s)	404, 858, 2126
BsnI	GGCC	7 cut(s)	362, 639, 1041, 1203, 1394, 1631, 2073
Bso31I	GGTCTC	1 cut(s)	110
BsoBI	CYCGRG	1 cut(s)	113
Bsp119I	TTCGAA	1 cut(s)	446
Bsp1286I	GDGCHC	2 cut(s)	489, 1174
Bsp1407I	TGTACA	1 cut(s)	618
Bsp143I	GATC	8 cut(s)	190, 657, 805, 1525, 1708, 1812, 1900, 2145
Bsp1720I	GCTNAGC	3 cut(s)	1553, 1640, 1914
Bsp19I	CCATGG	1 cut(s)	1180
BspACI	CCGC	8 cut(s)	54, 144, 178, 253, 453, 557, 580, 2182
BspANI	GGCC	7 cut(s)	362, 639, 1041, 1203, 1394, 1631, 2073
BspCNI	CTCAG	3 cut(s)	661, 1566, 1906
BspFNI	CGCG	1 cut(s)	453
BspLI	GGNNCC	3 cut(s)	10, 1171, 1824
BspMAI	CTGCAG	2 cut(s)	861, 973
BspMI	ACCTGC	1 cut(s)	1557
BspPI	GGATC	3 cut(s)	813, 1703, 1820
BspT104I	TTCGAA	1 cut(s)	446
BspT107I	GGYRCC	1 cut(s)	1169
BspTNI	GGTCTC	1 cut(s)	110
BsrGI	TGTACA	1 cut(s)	618
BsrI	ACTGG	3 cut(s)	640, 1174, 1882
BssECI	CCNNGG	6 cut(s)	357, 1119, 1120, 1180, 1395, 1396
BssMI	GATC	8 cut(s)	190, 657, 805, 1525, 1708, 1812, 1900, 2145
BssNI	GRCGYC	1 cut(s)	276
BssSI	CACGAG	2 cut(s)	736, 1138
BssT1I	CCWWGG	1 cut(s)	1180
Bst2BI	CACGAG	2 cut(s)	736, 1138
Bst2UI	CCWGG	5 cut(s)	313, 1121, 1318, 1397, 1827
Bst4CI	ACNGT	8 cut(s)	26, 477, 849, 979, 1055, 1222, 1250, 2027
Bst6I	CTCTTC	1 cut(s)	1701
BstACI	GRCGYC	1 cut(s)	276
BstAPI	GCANNNNNTGC	1 cut(s)	833
BstAUI	TGTACA	1 cut(s)	618
BstBI	TTCGAA	1 cut(s)	446
BstC8I	GCNNGC	6 cut(s)	178, 751, 869, 1156, 1726, 2037
BstDEI	CTNAG	8 cut(s)	669, 1150, 1159, 1553, 1640, 1689, 1914, 2099
BstDSI	CCRYGG	1 cut(s)	1180
BstF5I	GGATG	7 cut(s)	294, 807, 1299, 1312, 1392, 1777, 1835
BstFNI	CGCG	1 cut(s)	453
BstH2I	RGCGCY	1 cut(s)	930
BstHHI	GCGC	2 cut(s)	827, 929
BstKTI	GATC	8 cut(s)	193, 660, 808, 1528, 1711, 1815, 1903, 2148
BstMAI	GTCTC	6 cut(s)	110, 466, 968, 1318, 1358, 1698
BstMBI	GATC	8 cut(s)	190, 657, 805, 1525, 1708, 1812, 1900, 2145
BstMWI	GCNNNNNNNGC	6 cut(s)	159, 303, 335, 833, 1134, 1151
BstNI	CCWGG	5 cut(s)	313, 1121, 1318, 1397, 1827
BstNSI	RCATGY	2 cut(s)	691, 943
BstSCI	CCNGG	9 cut(s)	311, 338, 410, 1119, 1302, 1316, 1343, 1395, 1825
BstSFI	CTRYAG	4 cut(s)	857, 969, 1218, 1444
BstSLI	GKGCMC	2 cut(s)	489, 1174
BstUI	CGCG	1 cut(s)	453
BstV1I	GCAGC	5 cut(s)	32, 955, 983, 1697, 1741
BstV2I	GAAGAC	1 cut(s)	1275
BstX2I	RGATCY	1 cut(s)	1708
BstXI	CCANNNNNNTGG	1 cut(s)	1181
BstYI	RGATCY	1 cut(s)	1708
BsuRI	GGCC	7 cut(s)	362, 639, 1041, 1203, 1394, 1631, 2073
BtgI	CCRYGG	1 cut(s)	1180
BtsCI	GGATG	7 cut(s)	294, 807, 1299, 1312, 1392, 1777, 1835
BtsI	GCAGTG	1 cut(s)	428
BtsIMutI	CAGTG	3 cut(s)	428, 1060, 2032
BveI	ACCTGC	1 cut(s)	1557
Cac8I	GCNNGC	6 cut(s)	178, 751, 869, 1156, 1726, 2037
CfoI	GCGC	2 cut(s)	827, 929
Cfr13I	GGNCC	3 cut(s)	714, 1393, 1629
CseI	GACGC	1 cut(s)	1598
Csp6I	GTAC	6 cut(s)	334, 547, 619, 1051, 1502, 1859
CspCI	CAANNNNNGTGG	2 cut(s)	415, 450
CviQI	GTAC	6 cut(s)	334, 547, 619, 1051, 1502, 1859
DdeI	CTNAG	8 cut(s)	669, 1150, 1159, 1553, 1640, 1689, 1914, 2099
DpnI	GATC	8 cut(s)	192, 659, 807, 1527, 1710, 1814, 1902, 2147
DpnII	GATC	8 cut(s)	190, 657, 805, 1525, 1708, 1812, 1900, 2145
DraI	TTTAAA	1 cut(s)	2197
DraIII	CACNNNGTG	1 cut(s)	493
EaeI	YGGCCR	1 cut(s)	1039
Eam1104I	CTCTTC	1 cut(s)	1701
EarI	CTCTTC	1 cut(s)	1701
Eco130I	CCWWGG	1 cut(s)	1180
Eco147I	AGGCCT	1 cut(s)	1203
Eco31I	GGTCTC	1 cut(s)	110
Eco47I	GGWCC	1 cut(s)	714
Eco47III	AGCGCT	1 cut(s)	928
Eco88I	CYCGRG	1 cut(s)	113
EcoRII	CCWGG	5 cut(s)	311, 1119, 1316, 1395, 1825
EcoT14I	CCWWGG	1 cut(s)	1180
ErhI	CCWWGG	1 cut(s)	1180
FaqI	GGGAC	3 cut(s)	404, 858, 2126
FauI	CCCGC	5 cut(s)	185, 260, 446, 550, 573
FbaI	TGATCA	1 cut(s)	1525
Fnu4HI	GCNGC	5 cut(s)	46, 969, 997, 1686, 1755
FokI	GGATG	7 cut(s)	301, 814, 1286, 1319, 1399, 1784, 1842
Fsp4HI	GCNGC	5 cut(s)	46, 969, 997, 1686, 1755
FspBI	CTAG	2 cut(s)	1193, 1460
GlaI	GCGC	2 cut(s)	826, 928
GluI	GCNGC	5 cut(s)	46, 969, 997, 1686, 1755
GsaI	CCCAGC	2 cut(s)	333, 470
GsuI	CTGGAG	1 cut(s)	1940
HaeII	RGCGCY	1 cut(s)	930
HaeIII	GGCC	7 cut(s)	362, 639, 1041, 1203, 1394, 1631, 2073
HapII	CCGG	6 cut(s)	339, 412, 599, 729, 1303, 1345
HgaI	GACGC	1 cut(s)	1598
HhaI	GCGC	2 cut(s)	827, 929
Hin1I	GRCGYC	1 cut(s)	276
Hin6I	GCGC	2 cut(s)	825, 927
HinP1I	GCGC	2 cut(s)	825, 927
HincII	GTYRAC	2 cut(s)	937, 1990
HindII	GTYRAC	2 cut(s)	937, 1990
HindIII	AAGCTT	3 cut(s)	1126, 1156, 1585
HinfI	GANTC	9 cut(s)	448, 509, 701, 732, 1271, 1487, 1519, 1717, 1994
HpaII	CCGG	6 cut(s)	339, 412, 599, 729, 1303, 1345
HphI	GGTGA	2 cut(s)	194, 2140
Hpy166II	GTNNAC	5 cut(s)	714, 937, 1017, 1861, 1990
Hpy188I	TCNGA	9 cut(s)	98, 265, 288, 662, 790, 894, 1817, 1900, 1905
Hpy188III	TCNNGA	6 cut(s)	569, 736, 985, 1523, 1613, 1700
Hpy8I	GTNNAC	5 cut(s)	714, 937, 1017, 1861, 1990
HpyAV	CCTTC	6 cut(s)	130, 785, 1160, 1943, 2076, 2084
HpyCH4III	ACNGT	8 cut(s)	26, 477, 849, 979, 1055, 1222, 1250, 2027
HpyCH4IV	ACGT	3 cut(s)	276, 419, 815
HpyF10VI	GCNNNNNNNGC	6 cut(s)	159, 303, 335, 833, 1134, 1151
HpyF3I	CTNAG	8 cut(s)	669, 1150, 1159, 1553, 1640, 1689, 1914, 2099
HpySE526I	ACGT	3 cut(s)	276, 419, 815
Hsp92I	GRCGYC	1 cut(s)	276
HspAI	GCGC	2 cut(s)	825, 927
Ksp22I	TGATCA	1 cut(s)	1525
Kzo9I	GATC	8 cut(s)	190, 657, 805, 1525, 1708, 1812, 1900, 2145
LmnI	GCTCC	2 cut(s)	14, 395
Lsp1109I	GCAGC	5 cut(s)	32, 955, 983, 1697, 1741
MaeI	CTAG	2 cut(s)	1193, 1460
MaeII	ACGT	3 cut(s)	276, 419, 815
MaeIII	GTNAC	4 cut(s)	26, 683, 1237, 1417
MalI	GATC	8 cut(s)	192, 659, 807, 1527, 1710, 1814, 1902, 2147
MboI	GATC	8 cut(s)	190, 657, 805, 1525, 1708, 1812, 1900, 2145
MboII	GAAGA	9 cut(s)	907, 1070, 1175, 1280, 1578, 1586, 1688, 1958, 2020
MfeI	CAATTG	3 cut(s)	1368, 1620, 2040
MflI	RGATCY	1 cut(s)	1708
MhlI	GDGCHC	2 cut(s)	489, 1174
MlsI	TGGCCA	1 cut(s)	1041
MluCI	AATT	8 cut(s)	403, 1131, 1368, 1509, 1620, 1784, 2017, 2040
MluNI	TGGCCA	1 cut(s)	1041
MlyI	GAGTC	4 cut(s)	518, 695, 726, 1265
MmeI	TCCRAC	2 cut(s)	768, 1817
Mox20I	TGGCCA	1 cut(s)	1041
MroXI	GAANNNNTTC	1 cut(s)	2209
MscI	TGGCCA	1 cut(s)	1041
MseI	TTAA	9 cut(s)	167, 282, 1130, 1403, 1593, 1842, 2016, 2091, 2196
Msp20I	TGGCCA	1 cut(s)	1041
MspI	CCGG	6 cut(s)	339, 412, 599, 729, 1303, 1345
MspR9I	CCNGG	9 cut(s)	313, 340, 412, 1121, 1304, 1318, 1345, 1397, 1827
MunI	CAATTG	3 cut(s)	1368, 1620, 2040
MvaI	CCWGG	5 cut(s)	313, 1121, 1318, 1397, 1827
MvnI	CGCG	1 cut(s)	453
MwoI	GCNNNNNNNGC	6 cut(s)	159, 303, 335, 833, 1134, 1151
NciI	CCSGG	4 cut(s)	340, 412, 1304, 1345
NcoI	CCATGG	1 cut(s)	1180
NdeII	GATC	8 cut(s)	190, 657, 805, 1525, 1708, 1812, 1900, 2145
NlaIV	GGNNCC	3 cut(s)	10, 1171, 1824
NmeAIII	GCCGAG	4 cut(s)	138, 318, 382, 1583
NmuCI	GTSAC	1 cut(s)	683
NspI	RCATGY	2 cut(s)	691, 943
NspV	TTCGAA	1 cut(s)	446
PasI	CCCWGGG	2 cut(s)	1120, 1396
PceI	AGGCCT	1 cut(s)	1203
PciI	ACATGT	1 cut(s)	939
PdmI	GAANNNNTTC	1 cut(s)	2209
PfeI	GAWTC	5 cut(s)	448, 1487, 1519, 1717, 1994
PflMI	CCANNNNNTGG	1 cut(s)	711
PfoI	TCCNGGA	1 cut(s)	1302
PkrI	GCNGC	5 cut(s)	47, 970, 998, 1687, 1756
PleI	GAGTC	4 cut(s)	517, 695, 726, 1265
PpsI	GAGTC	4 cut(s)	517, 695, 726, 1265
PscI	ACATGT	1 cut(s)	939
PshBI	ATTAAT	1 cut(s)	1593
Psp6I	CCWGG	5 cut(s)	311, 1119, 1316, 1395, 1825
PspFI	CCCAGC	2 cut(s)	329, 466
PspGI	CCWGG	5 cut(s)	311, 1119, 1316, 1395, 1825
PspN4I	GGNNCC	3 cut(s)	10, 1171, 1824
PspPI	GGNCC	3 cut(s)	714, 1393, 1629
PstI	CTGCAG	2 cut(s)	861, 973
PsuI	RGATCY	1 cut(s)	1708
RsaI	GTAC	6 cut(s)	335, 548, 620, 1052, 1503, 1860
RsaNI	GTAC	6 cut(s)	334, 547, 619, 1051, 1502, 1859
SaqAI	TTAA	9 cut(s)	167, 282, 1130, 1403, 1593, 1842, 2016, 2091, 2196
SatI	GCNGC	5 cut(s)	46, 969, 997, 1686, 1755
Sau3AI	GATC	8 cut(s)	190, 657, 805, 1525, 1708, 1812, 1900, 2145
Sau96I	GGNCC	3 cut(s)	714, 1393, 1629
SchI	GAGTC	4 cut(s)	518, 695, 726, 1265
ScrFI	CCNGG	9 cut(s)	313, 340, 412, 1121, 1304, 1318, 1345, 1397, 1827
SduI	GDGCHC	2 cut(s)	489, 1174
SfcI	CTRYAG	4 cut(s)	857, 969, 1218, 1444
SfuI	TTCGAA	1 cut(s)	446
SinI	GGWCC	1 cut(s)	714
SmlI	CTYRAG	2 cut(s)	35, 1741
SmoI	CTYRAG	2 cut(s)	35, 1741
SpeI	ACTAGT	1 cut(s)	1459
Sse9I	AATT	8 cut(s)	403, 1131, 1368, 1509, 1620, 1784, 2017, 2040
SseBI	AGGCCT	1 cut(s)	1203
SsiI	CCGC	8 cut(s)	54, 144, 178, 253, 453, 557, 580, 2182
SspMI	CTAG	2 cut(s)	1193, 1460
StuI	AGGCCT	1 cut(s)	1203
StyD4I	CCNGG	9 cut(s)	311, 338, 410, 1119, 1302, 1316, 1343, 1395, 1825
StyI	CCWWGG	1 cut(s)	1180
TaaI	ACNGT	8 cut(s)	26, 477, 849, 979, 1055, 1222, 1250, 2027
TaiI	ACGT	3 cut(s)	279, 422, 818
TaqI	TCGA	6 cut(s)	189, 446, 551, 1266, 1699, 2083
TasI	AATT	8 cut(s)	403, 1131, 1368, 1509, 1620, 1784, 2017, 2040
TatI	WGTACW	4 cut(s)	546, 618, 1501, 1858
TfiI	GAWTC	5 cut(s)	448, 1487, 1519, 1717, 1994
Tru1I	TTAA	9 cut(s)	167, 282, 1130, 1403, 1593, 1842, 2016, 2091, 2196
Tru9I	TTAA	9 cut(s)	167, 282, 1130, 1403, 1593, 1842, 2016, 2091, 2196
TscAI	CASTG	3 cut(s)	435, 1060, 2032
TseFI	GTSAC	1 cut(s)	683
TseI	GCWGC	5 cut(s)	45, 968, 996, 1685, 1754
Tsp45I	GTSAC	1 cut(s)	683
TspGWI	ACGGA	3 cut(s)	226, 408, 2217
TspRI	CASTG	3 cut(s)	435, 1060, 2032
Van91I	CCANNNNNTGG	1 cut(s)	711
VpaK11BI	GGWCC	1 cut(s)	714
VspI	ATTAAT	1 cut(s)	1593
XceI	RCATGY	2 cut(s)	691, 943
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1833
XmnI	GAANNNNTTC	1 cut(s)	2209
XspI	CTAG	2 cut(s)	1193, 1460
ZraI	GACGTC	1 cut(s)	277

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.