Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002288

Physical Location & Seq

Genomic Coordinates Forward (+)

1698224 .. 1704838

Gene Structure 6615 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00078370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGGGCAACAAAATCACCGACGAACTATTATCCACCGTTCGTGCAATCGTCGATTCCGGCTACTCCGACATGGACATAATCCGAGCCCTACACATGGCCAACAACGACGTCACCGCCGCGATCAACATCATCTTCGACACCCCCACACCCAAATCTAAAGAAAGGTCAGATTTCGCGAGCAGTCTCAAAATCTCGAGTCCTGAAGTCGCGAATTCGAAGCCGAACGGCGGCGATTGCCTCGCCAGTTCAGGCGATGATGTAGTAGGTGACGTGGAGAGTTCGGCCGGCAGCGAGTGGTGGTTGGTGGGTTGCGGCGAGGTGGCAGGGTTATCGACGTGTAGAGGGAGGAGGATCAACCCCGGAGACGAAGTGGATTTCACTTTTCCTTTCAAGATTTCGCCGTCTCCCGGCAAGGTTTTCGGGAGGGGACGGCCGGCGGCGGCGTGTTCGGAGATTGTGAGGTTCTCCACGACGGATTCCGGAGAGATTGGACGAATACCGAAGGAATGGGCTCGGTGTCTGTTGCCACTTGTGAGAGATAAGAAGGTTAAAATCGAGGGGCATTGTAAATCTGCTCCTGATGTCTTGAGCATTATGGACACCATTCTCTTGTCTATAAGTGTGTATATCAATAGTGCTATGTTCCTTAACCAAAAGCAGACGTTACTTAAGGCAGCCAGTAATTCCACAGAGGAAACAGTGGTTCATCCTCTCCCGACATTGTTTCAGTTGCTAGGCCTAACTCCTTTCCAGAAGGCTGAATTTACTCCTGGTGACTTGTACACGAGAAAGCGCCCTTTAGACCAAAAGGACAGTTCTGGGCTTTGTGCCTCAATTATACATGCAAATAAGCATAAAAATCCTTCTAGAAATGAAGATGAAGTTGAAAATGAGGAATGTATTTCAGATGCTGAGGTTGATAACATTGTTGGGGTTGGAGATAGCTCTGAGTTAGAGATCCTAGCTGATGCTATGGGCCTGGGAAAGACGATTATGACAATAGCACTCCTTGTTGCTCATTCAGGACATGGATCATCAGGTAGTCAACCTGTGAGCCGGTCCTCCAGTGAAGATATTGAAGCCAGTGACATGTCAGACCATTCATCAAAACTCCCAAAGGCGTGGAAGGCAGAGATTGAATCTCATGTGCAACCTGGATCTGTATCAGTATATGTTCATTATGGACAAAGTAGACCAAGGGATGCGACTCTTTTAGCCCAAAGCGATGTTGTGATCACTACATATGGCGTTTTAGCATCAGAATTTTCTACAGAGAATTCTAAGGACAATGGGGGACTTTACTCAGTTAGTTGGTTTAGAGTGGTTCTTGATGAAGCGCACACCATAAAATCCTCTAAAAGCCAAATCTCTATTGCTGCTGCTGCTTTAGTTGCTGGTCGCCGATGGTGTCTTACTGGCACCCCTATACAGGATGCGATATCATTTACTGCTGAAATGATCTTACTTGTAGAAGTTATGTGGAATAAACTCATCCAGAAACCCTTTGAGGAGGGTGATGAGAGAGGGTTAAGGCTGGTTCAATCTATCTTAAAGACGATTATGTTAAGGAGAACCAAGTTTAGCACTGATCGTGAAGGCAGGCCTATTTTAGTCCTTCCTCCTGCTGATATTCAGGTGGTTTACTGTGAACTCACAGAAGCTGAAAAAGACTTTTATGAGGCCTTGTTTAAAAGATCAAAGGTGAAATTTGATCAATTTGTTCAGCAAGGACGGGTTCTTCATAATTATGCATCCATATTGGAATTACTTTTACGCCTTCGCCAATGTTGTGACCACCCATTTCTTGTGATGAGTCGAGGTGATACACAAGAATATTCAGATCTGAATAAGCTTGCAAGACATTTTCTAAAAGGCAGCCAGAATTCTGTGGAAGGGGAAGCCAAAGATGTGCCTTCACGGGCTTATGTTCAGGAAGTTGTTGAAGAAATGCGCAAGGGGGAACAGGGAGAGTGTCCCATATGTCTTGAAGCATTTGAAGATGCAGTATTGACAACTTGTGCTCATCGCTTATGCCGAGAATGTCTCTTGGCAAGTTGGCGGAATTCAACTTCTGGCTTATGTCCAGTTTGCAGGAAGAGTCTAAGTAAGCAAGATCTTATTACAGCACCAACTGACAGTCGGTTCCATGTTGATATTGAGAAAAACTGGGTGGAATCATCCAAAATAGTTATTCTTTTGCGTGAACTTGAATGTCTTCGTTCGTCAGGCTCAAAGAGCATTGTCTTCAGTCAGTGGACGGCCTTTCTGGACCTCCTGCAGATACCTCTTTCTCGGAGTAACATTCCATTTTTGCGCCTGGATGGGACTCTGAATCAACAGCAGCGCGAAAAAGTGTTAAAACAGTTTTCAGAAGACAGTGACATTCTGGTGTTGCTAATGTCACTGAAGGCCGGAGGAGTTGGAATAAATTTAACAGCAGCATCCAATGCCTTTGTCTTGGGAACAGTCGAGGAGAGAATGGAAGCAGTGCAAGCTCGTAAACAGCGGTTAATTTCTGGCGCTTTGACTGACCAAGAAGTTCGTTCGGCTCGCATAGAAGAACTAAAGATGCTTTTTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009987 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044764 GO:0045003 GO:0046483 GO:0050896 GO:0051704 GO:0051716 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HIRAN Helicase_C SNF2_N zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

860

Amino Acids

94.81

Weight (kDa)

5.64

Isoelectric Point (pI)

50.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UBA_RAD5A	PF24559	5 - 48	2e-24	DNA repair protein RAD5A UBA domain
HIRAN	PF08797	103 - 206	4.4e-15	HIRAN domain
SNF2-rel_dom	PF00176	320 - 601	1.8e-46	SNF2-related domain
zf-C3HC4_3	PF13920	655 - 702	4.2e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	657 - 697	4.2e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	657 - 698	9.5e-08	Ring finger domain
zf-C3HC4	PF00097	658 - 697	8.7e-09	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	658 - 695	9.4e-09	RING-type zinc-finger
Helicase_C	PF00271	729 - 832	4e-13	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22750
fragaria_vesca	FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660
malus_domestica	MD17G1018000.v1.1
prunus_persica	Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1
pyrus_communis	pycom17g01450
rosa_chinensis	RchiOBHm_Chr2g0174191 RchiOBHm_Chr4g0410751
rosa_laevigata	RLG00000022255 RLG00000022256 RLG00000029588
rosa_multiflora	Rmu_sc0000954.1_g000002 Rmu_ssc0000198.1_g000002
rosa_roxburghii	Rroxscaffold_2G00078340 Rroxscaffold_2G00078370 Rroxscaffold_5G00355500
rosa_rugosa	Rorug02G0575800 Rorug02G0575900 Rorug02G0576000 Rorug04G0376700
rosa_samantha	Rh2AG655200 Rh2BG666400 Rh2CG630600 Rh2DG681000 Rh4AG162800 Rh4BG161900 Rh4BG162000 Rh4DG158500 Rh7CG048600
rosa_wichuraiana	Rw2G053780 Rw4G013570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	111
Acc16I	TGCGCA	1 cut(s)	1952
AccB1I	GGYRCC	1 cut(s)	1418
AccI	GTMKAC	1 cut(s)	1192
AccII	CGCG	4 cut(s)	119, 176, 209, 2346
AccIII	TCCGGA	1 cut(s)	479
AciI	CCGC	8 cut(s)	114, 117, 228, 312, 437, 440, 2059, 2506
AclWI	GGATC	4 cut(s)	359, 952, 1039, 1165
AcoI	YGGCCR	3 cut(s)	96, 282, 431
AcsI	RAATTY	8 cut(s)	211, 761, 1262, 1276, 1706, 1882, 2062, 2428
AcuI	CTGAAG	3 cut(s)	222, 2230, 2426
AcyI	GRCGYC	1 cut(s)	108
AfaI	GTAC	1 cut(s)	782
AfiI	CCNNNNNNNGG	4 cut(s)	94, 227, 407, 1429
AflII	CTTAAG	1 cut(s)	668
AflIII	ACRYGT	2 cut(s)	335, 1089
AjiI	CACGTC	2 cut(s)	271, 336
AjnI	CCWGG	4 cut(s)	769, 978, 1153, 2316
AluBI	AGCT	5 cut(s)	945, 965, 1661, 1852, 2495
AluI	AGCT	5 cut(s)	945, 965, 1661, 1852, 2495
Alw21I	GWGCWC	1 cut(s)	2023
Alw26I	GTCTC	4 cut(s)	188, 357, 408, 2048
AlwI	GGATC	4 cut(s)	359, 952, 1039, 1165
AlwNI	CAGNNNCTG	2 cut(s)	911, 1661
Ama87I	CYCGRG	1 cut(s)	193
Aor13HI	TCCGGA	1 cut(s)	479
AoxI	GGCC	9 cut(s)	96, 282, 431, 736, 976, 1601, 1680, 2259, 2409
ApeKI	GCWGC	8 cut(s)	288, 674, 1376, 1379, 1382, 1875, 2341, 2438
ApoI	RAATTY	8 cut(s)	211, 761, 1262, 1276, 1706, 1882, 2062, 2428
Asp700I	GAANNNNTTC	1 cut(s)	2214
AspLEI	GCGC	6 cut(s)	795, 1339, 1953, 2316, 2346, 2522
AspS9I	GGNCC	3 cut(s)	976, 1059, 2269
AsuC2I	CCSGG	2 cut(s)	360, 408
AsuHPI	GGTGA	7 cut(s)	7, 103, 278, 785, 1526, 1714, 1832
AsuII	TTCGAA	1 cut(s)	215
AvaI	CYCGRG	1 cut(s)	193
AvaII	GGWCC	2 cut(s)	1059, 2269
BalI	TGGCCA	1 cut(s)	98
BanI	GGYRCC	1 cut(s)	1418
BanII	GRGCYC	2 cut(s)	88, 514
BauI	CACGAG	1 cut(s)	784
BbsI	GAAGAC	3 cut(s)	2207, 2236, 2379
Bbv12I	GWGCWC	1 cut(s)	2023
BbvCI	CCTCAGC	1 cut(s)	912
BbvI	GCAGC	8 cut(s)	300, 686, 1363, 1366, 1369, 1887, 2353, 2450
BccI	CCATC	2 cut(s)	1398, 2315
BceAI	ACGGC	4 cut(s)	241, 385, 446, 2274
BcgI	CGANNNNNNTGC	4 cut(s)	312, 346, 400, 434
BciT130I	CCWGG	4 cut(s)	771, 980, 1155, 2318
BclI	TGATCA	2 cut(s)	1233, 1711
BcnI	CCSGG	2 cut(s)	360, 408
BcoDI	GTCTC	4 cut(s)	188, 357, 408, 2048
BfaI	CTAG	3 cut(s)	734, 867, 962
BfmI	CTRYAG	2 cut(s)	1269, 2276
BfoI	RGCGCY	2 cut(s)	796, 2523
BfrI	CTTAAG	1 cut(s)	668
BglII	AGATCT	2 cut(s)	1840, 2113
Bme1390I	CCNGG	6 cut(s)	360, 408, 771, 980, 1155, 2318
Bme18I	GGWCC	2 cut(s)	1059, 2269
BmeT110I	CYCGRG	1 cut(s)	193
BmgBI	CACGTC	2 cut(s)	271, 336
BmgT120I	GGNCC	3 cut(s)	976, 1059, 2269
BmiI	GGNNCC	2 cut(s)	1420, 2144
BmrFI	CCNGG	6 cut(s)	360, 408, 771, 980, 1155, 2318
BmrI	ACTGGG	1 cut(s)	2176
BmsI	GCATC	9 cut(s)	898, 958, 1192, 1265, 1423, 1760, 1990, 2450, 2559
BmuI	ACTGGG	1 cut(s)	2176
BpiI	GAAGAC	3 cut(s)	2207, 2236, 2379
BplI	GAGNNNNNCTC	2 cut(s)	2028, 2060
BpmI	CTGGAG	1 cut(s)	1048
Bpu10I	CCTNAGC	1 cut(s)	912
Bpu14I	TTCGAA	1 cut(s)	215
BpuEI	CTTGAG	1 cut(s)	607
BpuMI	CCSGG	2 cut(s)	360, 408
BsaBI	GATNNNNATC	2 cut(s)	125, 1162
BsaHI	GRCGYC	1 cut(s)	108
BsaJI	CCNNGG	3 cut(s)	358, 979, 1196
BsaWI	WCCGGW	1 cut(s)	479
BsaXI	ACNNNNNCTCC	2 cut(s)	354, 384
Bsc4I	CCNNNNNNNGG	4 cut(s)	94, 227, 407, 1429
Bse118I	RCCGGY	3 cut(s)	284, 433, 1056
Bse1I	ACTGG	7 cut(s)	243, 678, 1065, 1083, 1420, 2084, 2171
Bse8I	GATNNNNATC	2 cut(s)	125, 1162
BseAI	TCCGGA	1 cut(s)	479
BseBI	CCWGG	4 cut(s)	771, 980, 1155, 2318
BseDI	CCNNGG	3 cut(s)	358, 979, 1196
BseGI	GGATG	8 cut(s)	706, 1207, 1438, 1491, 1751, 2177, 2326, 2441
BseJI	GATNNNNATC	2 cut(s)	125, 1162
BseLI	CCNNNNNNNGG	4 cut(s)	94, 227, 407, 1429
BseMII	CTCAG	3 cut(s)	903, 939, 1317
BseNI	ACTGG	7 cut(s)	243, 678, 1065, 1083, 1420, 2084, 2171
BseRI	GAGGAG	4 cut(s)	361, 1523, 2430, 2486
BseX3I	CGGCCG	2 cut(s)	282, 431
BseXI	GCAGC	8 cut(s)	300, 686, 1363, 1366, 1369, 1887, 2353, 2450
Bsh1236I	CGCG	4 cut(s)	119, 176, 209, 2346
Bsh1285I	CGRYCG	2 cut(s)	285, 434
BshFI	GGCC	9 cut(s)	98, 284, 433, 738, 978, 1603, 1682, 2261, 2411
BshNI	GGYRCC	1 cut(s)	1418
BsiEI	CGRYCG	2 cut(s)	285, 434
BsiHKAI	GWGCWC	1 cut(s)	2023
BsiHKCI	CYCGRG	1 cut(s)	193
BsiSI	CCGG	8 cut(s)	57, 285, 360, 408, 434, 480, 1057, 2412
BslFI	GGGAC	4 cut(s)	441, 1308, 1959, 2338
BslI	CCNNNNNNNGG	4 cut(s)	94, 227, 407, 1429
BsmAI	GTCTC	4 cut(s)	188, 357, 408, 2048
BsmBI	CGTCTC	2 cut(s)	357, 408
BsmFI	GGGAC	4 cut(s)	441, 1308, 1959, 2338
BsnI	GGCC	9 cut(s)	98, 284, 433, 738, 978, 1603, 1682, 2261, 2411
BsoBI	CYCGRG	1 cut(s)	193
Bsp119I	TTCGAA	1 cut(s)	215
Bsp1286I	GDGCHC	3 cut(s)	88, 514, 2023
Bsp13I	TCCGGA	1 cut(s)	479
Bsp1407I	TGTACA	1 cut(s)	780
Bsp68I	TCGCGA	2 cut(s)	176, 209
BspACI	CCGC	8 cut(s)	114, 117, 228, 312, 437, 440, 2059, 2506
BspANI	GGCC	9 cut(s)	98, 284, 433, 738, 978, 1603, 1682, 2261, 2411
BspCNI	CTCAG	3 cut(s)	904, 940, 1316
BspEI	TCCGGA	1 cut(s)	479
BspFNI	CGCG	4 cut(s)	119, 176, 209, 2346
BspLI	GGNNCC	2 cut(s)	1420, 2144
BspMAI	CTGCAG	1 cut(s)	2280
BspPI	GGATC	4 cut(s)	359, 952, 1039, 1165
BspT104I	TTCGAA	1 cut(s)	215
BspT107I	GGYRCC	1 cut(s)	1418
BspTI	CTTAAG	1 cut(s)	668
BsrFI	RCCGGY	3 cut(s)	284, 433, 1056
BsrGI	TGTACA	1 cut(s)	780
BsrI	ACTGG	7 cut(s)	243, 678, 1065, 1083, 1420, 2084, 2171
BssAI	RCCGGY	3 cut(s)	284, 433, 1056
BssECI	CCNNGG	3 cut(s)	358, 979, 1196
BssNI	GRCGYC	1 cut(s)	108
BssSI	CACGAG	1 cut(s)	784
BssT1I	CCWWGG	1 cut(s)	1196
Bst2BI	CACGAG	1 cut(s)	784
Bst2UI	CCWGG	4 cut(s)	771, 980, 1155, 2318
Bst4CI	ACNGT	8 cut(s)	37, 700, 815, 1646, 2138, 2364, 2378, 2467
Bst6I	CTCTTC	1 cut(s)	2090
BstACI	GRCGYC	1 cut(s)	108
BstAFI	CTTAAG	1 cut(s)	668
BstAPI	GCANNNNNTGC	1 cut(s)	2447
BstAUI	TGTACA	1 cut(s)	780
BstBI	TTCGAA	1 cut(s)	215
BstC8I	GCNNGC	7 cut(s)	178, 286, 435, 1601, 1854, 2493, 2551
BstDEI	CTNAG	5 cut(s)	912, 948, 1281, 1303, 2102
BstF5I	GGATG	8 cut(s)	706, 1207, 1438, 1491, 1751, 2177, 2326, 2441
BstFNI	CGCG	4 cut(s)	119, 176, 209, 2346
BstH2I	RGCGCY	2 cut(s)	796, 2523
BstHHI	GCGC	6 cut(s)	795, 1339, 1953, 2316, 2346, 2522
BstMAI	GTCTC	4 cut(s)	188, 357, 408, 2048
BstMCI	CGRYCG	2 cut(s)	285, 434
BstMWI	GCNNNNNNNGC	7 cut(s)	234, 1127, 1382, 1391, 2235, 2447, 2492
BstNI	CCWGG	4 cut(s)	771, 980, 1155, 2318
BstNSI	RCATGY	2 cut(s)	845, 1093
BstSCI	CCNGG	6 cut(s)	358, 406, 769, 978, 1153, 2316
BstSFI	CTRYAG	2 cut(s)	1269, 2276
BstUI	CGCG	4 cut(s)	119, 176, 209, 2346
BstV1I	GCAGC	8 cut(s)	300, 686, 1363, 1366, 1369, 1887, 2353, 2450
BstV2I	GAAGAC	3 cut(s)	2207, 2236, 2379
BstX2I	RGATCY	4 cut(s)	957, 1157, 1840, 2113
BstXI	CCANNNNNNTGG	1 cut(s)	1122
BstYI	RGATCY	4 cut(s)	957, 1157, 1840, 2113
BstZI	CGGCCG	2 cut(s)	282, 431
BsuRI	GGCC	9 cut(s)	98, 284, 433, 738, 978, 1603, 1682, 2261, 2411
BtgZI	GCGATG	3 cut(s)	267, 1239, 2009
BtrI	CACGTC	2 cut(s)	271, 336
BtsCI	GGATG	8 cut(s)	706, 1207, 1438, 1491, 1751, 2177, 2326, 2441
BtsI	GCAGTG	1 cut(s)	2493
BtsIMutI	CAGTG	8 cut(s)	705, 1072, 1090, 1584, 2258, 2383, 2402, 2493
BtuMI	TCGCGA	2 cut(s)	176, 209
Cac8I	GCNNGC	7 cut(s)	178, 286, 435, 1601, 1854, 2493, 2551
CaiI	CAGNNNCTG	2 cut(s)	911, 1661
CfoI	GCGC	6 cut(s)	795, 1339, 1953, 2316, 2346, 2522
Cfr10I	RCCGGY	3 cut(s)	284, 433, 1056
Cfr13I	GGNCC	3 cut(s)	976, 1059, 2269
Csp6I	GTAC	1 cut(s)	781
CviAII	CATG	7 cut(s)	70, 94, 842, 1028, 1090, 1145, 2147
CviQI	GTAC	1 cut(s)	781
DdeI	CTNAG	5 cut(s)	912, 948, 1281, 1303, 2102
DraI	TTTAAA	1 cut(s)	1690
EaeI	YGGCCR	3 cut(s)	96, 282, 431
EagI	CGGCCG	2 cut(s)	282, 431
Eam1104I	CTCTTC	1 cut(s)	2090
EarI	CTCTTC	1 cut(s)	2090
EciI	GGCGGA	1 cut(s)	2074
EclXI	CGGCCG	2 cut(s)	282, 431
Eco130I	CCWWGG	1 cut(s)	1196
Eco147I	AGGCCT	3 cut(s)	738, 1603, 1682
Eco24I	GRGCYC	2 cut(s)	88, 514
Eco32I	GATATC	1 cut(s)	1440
Eco47I	GGWCC	2 cut(s)	1059, 2269
Eco52I	CGGCCG	2 cut(s)	282, 431
Eco57I	CTGAAG	3 cut(s)	222, 2230, 2426
Eco88I	CYCGRG	1 cut(s)	193
EcoRI	GAATTC	4 cut(s)	211, 1276, 1882, 2062
EcoRII	CCWGG	4 cut(s)	769, 978, 1153, 2316
EcoRV	GATATC	1 cut(s)	1440
EcoT14I	CCWWGG	1 cut(s)	1196
EcoT22I	ATGCAT	1 cut(s)	1753
EcoT38I	GRGCYC	2 cut(s)	88, 514
ErhI	CCWWGG	1 cut(s)	1196
Esp3I	CGTCTC	2 cut(s)	357, 408
FaeI	CATG	7 cut(s)	73, 97, 845, 1031, 1093, 1148, 2150
FaqI	GGGAC	4 cut(s)	441, 1308, 1959, 2338
FatI	CATG	7 cut(s)	69, 93, 841, 1027, 1089, 1144, 2146
FauNDI	CATATG	2 cut(s)	1243, 1979
FbaI	TGATCA	2 cut(s)	1233, 1711
FblI	GTMKAC	1 cut(s)	1192
FokI	GGATG	8 cut(s)	693, 1214, 1445, 1478, 1738, 2164, 2333, 2428
FriOI	GRGCYC	2 cut(s)	88, 514
FspBI	CTAG	3 cut(s)	734, 867, 962
FspI	TGCGCA	1 cut(s)	1952
GlaI	GCGC	6 cut(s)	794, 1338, 1952, 2315, 2345, 2521
GsuI	CTGGAG	1 cut(s)	1048
HaeII	RGCGCY	2 cut(s)	796, 2523
HaeIII	GGCC	9 cut(s)	98, 284, 433, 738, 978, 1603, 1682, 2261, 2411
HapII	CCGG	8 cut(s)	57, 285, 360, 408, 434, 480, 1057, 2412
HhaI	GCGC	6 cut(s)	795, 1339, 1953, 2316, 2346, 2522
Hin1I	GRCGYC	1 cut(s)	108
Hin1II	CATG	7 cut(s)	73, 97, 845, 1031, 1093, 1148, 2150
Hin6I	GCGC	6 cut(s)	793, 1337, 1951, 2314, 2344, 2520
HinP1I	GCGC	6 cut(s)	793, 1337, 1951, 2314, 2344, 2520
HincII	GTYRAC	1 cut(s)	1046
HindII	GTYRAC	1 cut(s)	1046
HindIII	AAGCTT	1 cut(s)	1850
HpaII	CCGG	8 cut(s)	57, 285, 360, 408, 434, 480, 1057, 2412
HphI	GGTGA	7 cut(s)	7, 103, 278, 785, 1526, 1714, 1832
Hpy166II	GTNNAC	8 cut(s)	783, 1046, 1193, 1642, 1649, 2204, 2256, 2501
Hpy8I	GTNNAC	8 cut(s)	783, 1046, 1193, 1642, 1649, 2204, 2256, 2501
Hpy99I	CGWCG	5 cut(s)	23, 53, 110, 337, 475
HpyCH4III	ACNGT	8 cut(s)	37, 700, 815, 1646, 2138, 2364, 2378, 2467
HpyCH4IV	ACGT	4 cut(s)	108, 270, 335, 662
HpyCH4V	TGCA	9 cut(s)	44, 845, 1150, 1751, 1856, 2003, 2091, 2278, 2491
HpyF10VI	GCNNNNNNNGC	7 cut(s)	234, 1127, 1382, 1391, 2235, 2447, 2492
HpyF3I	CTNAG	5 cut(s)	912, 948, 1281, 1303, 2102
HpySE526I	ACGT	4 cut(s)	108, 270, 335, 662
Hsp92I	GRCGYC	1 cut(s)	108
Hsp92II	CATG	7 cut(s)	73, 97, 845, 1031, 1093, 1148, 2150
HspAI	GCGC	6 cut(s)	793, 1337, 1951, 2314, 2344, 2520
Kpn2I	TCCGGA	1 cut(s)	479
KroI	GCCGGC	2 cut(s)	284, 433
KroNI	GCCGGC	2 cut(s)	286, 435
Ksp22I	TGATCA	2 cut(s)	1233, 1711
LmnI	GCTCC	1 cut(s)	580
Lsp1109I	GCAGC	8 cut(s)	300, 686, 1363, 1366, 1369, 1887, 2353, 2450
LweI	GCATC	9 cut(s)	898, 958, 1192, 1265, 1423, 1760, 1990, 2450, 2559
MaeI	CTAG	3 cut(s)	734, 867, 962
MaeII	ACGT	4 cut(s)	108, 270, 335, 662
MaeIII	GTNAC	9 cut(s)	109, 266, 663, 773, 1085, 1790, 2297, 2378, 2400
MflI	RGATCY	4 cut(s)	957, 1157, 1840, 2113
MhlI	GDGCHC	3 cut(s)	88, 514, 2023
MlsI	TGGCCA	1 cut(s)	98
MluNI	TGGCCA	1 cut(s)	98
MlyI	GAGTC	5 cut(s)	205, 1201, 1822, 2107, 2320
MmeI	TCCRAC	3 cut(s)	90, 916, 2401
Mox20I	TGGCCA	1 cut(s)	98
Mph1103I	ATGCAT	1 cut(s)	1753
MroI	TCCGGA	1 cut(s)	479
MroNI	GCCGGC	2 cut(s)	284, 433
MroXI	GAANNNNTTC	1 cut(s)	2214
MscI	TGGCCA	1 cut(s)	98
MslI	CAYNNNNRTG	2 cut(s)	1746, 2387
Msp20I	TGGCCA	1 cut(s)	98
MspA1I	CMGCKG	1 cut(s)	2506
MspCI	CTTAAG	1 cut(s)	668
MspI	CCGG	8 cut(s)	57, 285, 360, 408, 434, 480, 1057, 2412
MspR9I	CCNGG	6 cut(s)	360, 408, 771, 980, 1155, 2318
MvaI	CCWGG	4 cut(s)	771, 980, 1155, 2318
MvnI	CGCG	4 cut(s)	119, 176, 209, 2346
MwoI	GCNNNNNNNGC	7 cut(s)	234, 1127, 1382, 1391, 2235, 2447, 2492
NaeI	GCCGGC	2 cut(s)	286, 435
NciI	CCSGG	2 cut(s)	360, 408
NdeI	CATATG	2 cut(s)	1243, 1979
NgoMIV	GCCGGC	2 cut(s)	284, 433
NlaIII	CATG	7 cut(s)	73, 97, 845, 1031, 1093, 1148, 2150
NlaIV	GGNNCC	2 cut(s)	1420, 2144
NmeAIII	GCCGAG	1 cut(s)	2060
NmuCI	GTSAC	7 cut(s)	109, 266, 773, 1085, 1790, 2378, 2400
NruI	TCGCGA	2 cut(s)	176, 209
NsbI	TGCGCA	1 cut(s)	1952
NsiI	ATGCAT	1 cut(s)	1753
NspI	RCATGY	2 cut(s)	845, 1093
NspV	TTCGAA	1 cut(s)	215
PaeR7I	CTCGAG	1 cut(s)	193
PceI	AGGCCT	3 cut(s)	738, 1603, 1682
PciI	ACATGT	1 cut(s)	1089
PcsI	WCGNNNNNNNCGW	1 cut(s)	2032
PdiI	GCCGGC	2 cut(s)	286, 435
PdmI	GAANNNNTTC	1 cut(s)	2214
PfeI	GAWTC	5 cut(s)	53, 476, 1139, 2174, 2332
PleI	GAGTC	5 cut(s)	204, 1201, 1821, 2106, 2320
PpsI	GAGTC	5 cut(s)	204, 1201, 1821, 2106, 2320
PscI	ACATGT	1 cut(s)	1089
Psp6I	CCWGG	4 cut(s)	769, 978, 1153, 2316
PspGI	CCWGG	4 cut(s)	769, 978, 1153, 2316
PspN4I	GGNNCC	2 cut(s)	1420, 2144
PspPI	GGNCC	3 cut(s)	976, 1059, 2269
PstI	CTGCAG	1 cut(s)	2280
PstNI	CAGNNNCTG	2 cut(s)	911, 1661
PsuI	RGATCY	4 cut(s)	957, 1157, 1840, 2113
RruI	TCGCGA	2 cut(s)	176, 209
RsaI	GTAC	1 cut(s)	782
RsaNI	GTAC	1 cut(s)	781
RseI	CAYNNNNRTG	2 cut(s)	1746, 2387
Sau96I	GGNCC	3 cut(s)	976, 1059, 2269
SchI	GAGTC	5 cut(s)	205, 1201, 1822, 2107, 2320
ScrFI	CCNGG	6 cut(s)	360, 408, 771, 980, 1155, 2318
SduI	GDGCHC	3 cut(s)	88, 514, 2023
SfaNI	GCATC	9 cut(s)	898, 958, 1192, 1265, 1423, 1760, 1990, 2450, 2559
SfcI	CTRYAG	2 cut(s)	1269, 2276
Sfr274I	CTCGAG	1 cut(s)	193
SfuI	TTCGAA	1 cut(s)	215
SinI	GGWCC	2 cut(s)	1059, 2269
SlaI	CTCGAG	1 cut(s)	193
SmiMI	CAYNNNNRTG	2 cut(s)	1746, 2387
SmlI	CTYRAG	3 cut(s)	193, 586, 668
SmoI	CTYRAG	3 cut(s)	193, 586, 668
SseBI	AGGCCT	3 cut(s)	738, 1603, 1682
SsiI	CCGC	8 cut(s)	114, 117, 228, 312, 437, 440, 2059, 2506
SspI	AATATT	1 cut(s)	1835
SspMI	CTAG	3 cut(s)	734, 867, 962
StuI	AGGCCT	3 cut(s)	738, 1603, 1682
StyD4I	CCNGG	6 cut(s)	358, 406, 769, 978, 1153, 2316
StyI	CCWWGG	1 cut(s)	1196
TaaI	ACNGT	8 cut(s)	37, 700, 815, 1646, 2138, 2364, 2378, 2467
TaiI	ACGT	4 cut(s)	111, 273, 338, 665
TaqI	TCGA	8 cut(s)	51, 135, 194, 215, 332, 555, 1816, 2469
TatI	WGTACW	1 cut(s)	780
TauI	GCSGC	5 cut(s)	119, 231, 315, 440, 443
TfiI	GAWTC	5 cut(s)	53, 476, 1139, 2174, 2332
TscAI	CASTG	8 cut(s)	705, 1072, 1090, 1591, 2258, 2383, 2409, 2493
TseFI	GTSAC	7 cut(s)	109, 266, 773, 1085, 1790, 2378, 2400
TseI	GCWGC	8 cut(s)	288, 674, 1376, 1379, 1382, 1875, 2341, 2438
Tsp45I	GTSAC	7 cut(s)	109, 266, 773, 1085, 1790, 2378, 2400
TspDTI	ATGAA	7 cut(s)	695, 888, 894, 1092, 1166, 1347, 1730
TspGWI	ACGGA	1 cut(s)	488
TspRI	CASTG	8 cut(s)	705, 1072, 1090, 1591, 2258, 2383, 2409, 2493
Vha464I	CTTAAG	1 cut(s)	668
VpaK11BI	GGWCC	2 cut(s)	1059, 2269
XapI	RAATTY	8 cut(s)	211, 761, 1262, 1276, 1706, 1882, 2062, 2428
XbaI	TCTAGA	1 cut(s)	866
XceI	RCATGY	2 cut(s)	845, 1093
XhoI	CTCGAG	1 cut(s)	193
XmiI	GTMKAC	1 cut(s)	1192
XmnI	GAANNNNTTC	1 cut(s)	2214
XspI	CTAG	3 cut(s)	734, 867, 962
ZraI	GACGTC	1 cut(s)	109
Zsp2I	ATGCAT	1 cut(s)	1753

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.