Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0002288

Physical Location & Seq

Genomic Coordinates Reverse (-)

38693255 .. 38699848

Gene Structure 6594 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g52660.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3051 bp

ATGGGCAACAAAATCACCGACGAGCTCTTATCCACCGTCCGTTCAATCGTCGATTCCGGCTACTCCGACATGGACATAATCCGAGCCCTTCACATGGCCAACAACGACGTCACCGCCGCAATCAACATCATCTTCGACACCCCCACTCTCAAATCCTCCAAAGCAAGACCCGATTTCCCCAACAGTCCCAGAATTCCGATCCCCGAAGCCGCGAATTCGAAGCAGAACGGCGGCGATTGCGCCGCCAGTTCCGGCGATGACGTCGTCGCTGACGTGGAGAGGCCGCTCGGCGGCAGCGAGGAGTGGTGGTTGGTGGGTTGCGGCGAGGTGGCGGGTTTGTCGACGTGCAGAGGGAGGAAGATCAAGGCCGGAGACGTAGTGGATTTCACATTTCCTCAAAAGATTTCGCCGTCTCCCGGCAAGGCTTTTGGCCGGGGACGGCCGGCGGCGGCGGCGTGTTCGGAGATTGTGAGGTTCTCTACGACGGATTCCGGGGAGATTGGACGAATACCGAAGGAATGGGCTCGGTGTCTGTTGCCACTTGTGAGAGATAAGAAGGTCAAAATTGAGGGGCATTGTAAATCTGCTCCGGATGTATTGAGCATTATGGACACCATTCTCTTGTCTATAAGTGTGTATATCAATAGTTCTATGTTCCTTAAGCAAAAGCAGACCTCGCTTAAGGTAGCGAGTAATTCCACAGAGGAAACAGTAGTTCATCCTCTCCCGACATTGTTCCAGTTGCTAGGCCTAACTCCTTTCCAGAAGGCAGAATTTACTCCCGGTGACTTGTACACGAGAAAGCGCCCTTTAGACCAAAAGGACAGTTCTGGGGTTTGTGCCTCAATTGTACATGCAATAAAGCATAAAAATCCTTCTATAAATGAAGGTGAAGTTGAAAACGAGGAATGTATTTCGGATGCTGAGGTTGATAACATCGTTGGGGTTGGAGATTGCTCTGAGTTAGAGGAAATGGATCCTCCTGCTGCACTTCTGTGTGAACTTCGTCCGTACCAAAAGCAGGCTCTTAATTGGATGATCCAACTAGAAAAGGGTAAATGCATGGATGAGGGAGCAATGACTCTTCACCCTGGTTGGGAGGCATATCGTCTTGCAGACAAGAGGGACCGTATTATATATCTGAATGCATTTTCTGGTGATGCAACAACAGAATTTCCAAGCGCACTTCAAATGGCCAGAGGAGGAATCCTAGCTGATGCTATGGGCCTGGGGAAGACGATTATGACAATAGCACTCCTTGTTGCTCATTCAGGACATGGACCGTCGGGTAGTCAACCTATAAGCCGGTCCTCCTTTGAAGATATTGAAGCCAGTGACATGTCAGACCATTCCTCAAAACTCCCAAAGGCGGTCACAAAGTTTTCAGGATTTGATAAGTTGATGAAGCAAAAAAACATGCTTGCATATGGTGGCAGTTTAATTGTATGTCCTATGACCCTTCTAGGCCAGTGGAAGGCAGAAATTGAATCTCATGTGCGTCCTGGATGTGTATCAGTATATGTTCACTATGGACAAAGTAGACCAAAGGATGCAAATCTCTTAGCTCAAAGCAATGTTGTGATCACTACATATGGAGTTTTAGCGTCAGAATTTTCCACAGAGAATTCTAAGGACAATGGGGGACTTTTCTCAGTTAGTTGGTTTAGAGTGGTTCTTGATGAGGCACATACCATAAAATCCTCTAAAAGCCAAATCTCTATTGCTGCTGCTGCTTTAGTTGCTGGTCGCCGATGGTGTCTTACTGGCACCCCTATACAGAACAATTTGGAGGACATTTATAGTCTACTCCGATTTTTGAGGGTAGAACCTTGGGCAAATTGGGCTTGGTGGAATAAACTCATCCAGAAACCCTTTGAGGAAGGTGATGAGAGAGGGTTAAAGCTGGTTCAATCTATCTTGAAGACGATTATGTTAAGGAGAACCAAGTTTAGTACCGATTGCGAAGGCAGGCCTATATTAGTCCTTCCTCCTGCTGATATTCAGGTGATATACTGTGAACTCACAGAAGCAGAAAAGGACTTTTATGAGGCCTTGTTTAAAAGATCAAAGGTGAAATTTGATCAATTTGTTGAGCAAGGACGGGTTCTTCATAATTATGCATCCATATTGGAATTACTTTTACGCCTTCGCCAATGTTGCGATCATCCATTTCTTGTGATGAGTCGAGGTGATACACAAGAATATTCAGATCTGAATAAGCTTGCTAGACGGTTCTTAAAAGGCAGCCAGAATTCTGTGGAAGGGGAAGCCAAAAATTTACCTTCACGGGCTTATGTTCAAGAAGTTGTTGAAGAAATACGCAAGGGGGAACAGGGAGAGTGTCCCATATGTCTTGAAGCATTTGAAGATGCAGTATTGACACCTTGTGCTCATCGCTTATGCCGTGAATGTCTCTTGGCTAGTTGGCGGAATTCAACTTCCGGCTTATGTCCGGTTTGCAGGAAGAGTGTAAGCAAGCAAGATCTTATTACAGCACCAACTGAGAGTCGGTTTCATGTTGATATTGAGAAAAACTGGGTGGAATCATCCAAAATAGTTATTCTTTTGCGTGAGCTTGAATGTCTTCGTTCATCAGGCTCAAAGAGCATTGTCTTCAGCCAGTGGACGGCCTTTCTAGACCTCCTGCAGATATCTCTTTCTCGGAGTAACATTCCATATCTTCGCCTGGATGGGACTCTCAGTCAACAGCAGCGCGAGAAAGTGTTAAAACAGTTTTCAGAAGACAGTGACATTCTGGTGTTGCTAATGTCACTGAAGGCCGGAGGAGTTGGAATAAATCTAACAGCAGCATCCAATGCCTTTGTCTTGGATCCATGGTGGAATCCAGCTGTAGAGGAACAAGCTGTCATGCGTATTCATCGCATTGGACAAACAAAAAGTGTGATGATCAAACGATTTATCGTGAAGGGAACGGTCGAGGAAAGAATGGAAGCAGTTCAAGCTCGTAAACAGCGGTTAATTTCTGGTGCCTTAACAGACCAAGAAGTTCGAACAGCTCGCATAGAGGAACTAAAGATGCTTTTTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009987 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044764 GO:0045003 GO:0046483 GO:0050896 GO:0051704 GO:0051716 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HIRAN Helicase_C SNF2_N zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

1017

Amino Acids

112.75

Weight (kDa)

6.55

Isoelectric Point (pI)

46.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UBA_RAD5A	PF24559	5 - 48	5.6e-25	DNA repair protein RAD5A UBA domain
HIRAN	PF08797	106 - 210	1.6e-15	HIRAN domain
SNF2-rel_dom	PF00176	338 - 724	2.2e-74	SNF2-related domain
DEAD	PF00270	408 - 598	3.4e-06	DEAD/DEAH box helicase
zf-C3HC4_3	PF13920	778 - 826	1.9e-09	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	780 - 820	1.5e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	780 - 821	3.7e-08	Ring finger domain
zf-RING_UBOX	PF13445	781 - 818	3e-09	RING-type zinc-finger
zf-C3HC4	PF00097	781 - 820	5.1e-09	Zinc finger, C3HC4 type (RING finger)
Prok-RING_4	PF14447	781 - 828	2.7e-06	Prokaryotic RING finger family 4
Helicase_C	PF00271	852 - 963	2.8e-21	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22750
fragaria_vesca	FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660
malus_domestica	MD17G1018000.v1.1
prunus_persica	Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1
pyrus_communis	pycom17g01450
rosa_chinensis	RchiOBHm_Chr2g0174191 RchiOBHm_Chr4g0410751
rosa_laevigata	RLG00000022255 RLG00000022256 RLG00000029588
rosa_multiflora	Rmu_sc0000954.1_g000002 Rmu_ssc0000198.1_g000002
rosa_roxburghii	Rroxscaffold_2G00078340 Rroxscaffold_2G00078370 Rroxscaffold_5G00355500
rosa_rugosa	Rorug02G0575800 Rorug02G0575900 Rorug02G0576000 Rorug04G0376700
rosa_samantha	Rh2AG655200 Rh2BG666400 Rh2CG630600 Rh2DG681000 Rh4AG162800 Rh4BG161900 Rh4BG162000 Rh4DG158500 Rh7CG048600
rosa_wichuraiana	Rw2G053780 Rw4G013570

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	111, 264
AccB1I	GGYRCC	2 cut(s)	1766, 2987
AccBSI	CCGCTC	1 cut(s)	286
AccI	GTMKAC	3 cut(s)	341, 1540, 1804
AccII	CGCG	2 cut(s)	212, 2715
AccIII	TCCGGA	1 cut(s)	589
AclWI	GGATC	6 cut(s)	193, 971, 984, 1033, 2825, 2838
AcoI	YGGCCR	4 cut(s)	96, 430, 440, 1194
AcuI	CTGAAG	2 cut(s)	2599, 2795
AcyI	GRCGYC	2 cut(s)	108, 261
AdeI	CACNNNGTG	1 cut(s)	2387
AfaI	GTAC	4 cut(s)	794, 852, 1013, 1954
AfiI	CCNNNNNNNGG	8 cut(s)	94, 290, 416, 1021, 1096, 1369, 1474, 2626
AflII	CTTAAG	2 cut(s)	659, 680
AflIII	ACRYGT	1 cut(s)	1338
AhdI	GACNNNNNGTC	1 cut(s)	2700
AjiI	CACGTC	2 cut(s)	274, 345
AjnI	CCWGG	4 cut(s)	1090, 1227, 1501, 2685
AleI	CACNNNNGTG	1 cut(s)	994
Alw21I	GWGCWC	2 cut(s)	27, 2392
Alw26I	GTCTC	3 cut(s)	366, 417, 2417
AlwI	GGATC	6 cut(s)	193, 971, 984, 1033, 2825, 2838
Aor13HI	TCCGGA	1 cut(s)	589
ApeKI	GCWGC	8 cut(s)	294, 986, 1724, 1727, 1730, 2244, 2710, 2807
Asp700I	GAANNNNTTC	2 cut(s)	2583, 2955
AspLEI	GCGC	4 cut(s)	242, 807, 1184, 2715
AspS9I	GGNCC	4 cut(s)	1126, 1225, 1280, 1308
AsuC2I	CCSGG	4 cut(s)	417, 434, 493, 783
AsuII	TTCGAA	2 cut(s)	218, 3010
AvaII	GGWCC	3 cut(s)	1126, 1280, 1308
BalI	TGGCCA	2 cut(s)	98, 1196
BamHI	GGATCC	2 cut(s)	976, 2830
BanI	GGYRCC	2 cut(s)	1766, 2987
BanII	GRGCYC	3 cut(s)	27, 88, 526
BauI	CACGAG	1 cut(s)	796
BbsI	GAAGAC	5 cut(s)	1241, 1928, 2576, 2605, 2748
Bbv12I	GWGCWC	2 cut(s)	27, 2392
BbvCI	CCTCAGC	1 cut(s)	924
BbvI	GCAGC	8 cut(s)	306, 973, 1711, 1714, 1717, 2256, 2722, 2819
BccI	CCATC	2 cut(s)	1746, 2684
BceAI	ACGGC	5 cut(s)	244, 394, 455, 2388, 2643
BciT130I	CCWGG	4 cut(s)	1092, 1229, 1503, 2687
BclI	TGATCA	3 cut(s)	1581, 2080, 2907
BcnI	CCSGG	4 cut(s)	417, 434, 493, 783
BcoDI	GTCTC	3 cut(s)	366, 417, 2417
BfaI	CTAG	7 cut(s)	746, 1046, 1211, 1463, 2226, 2421, 2636
BfmI	CTRYAG	2 cut(s)	2645, 2850
BfoI	RGCGCY	1 cut(s)	808
BfrI	CTTAAG	2 cut(s)	659, 680
BglII	AGATCT	2 cut(s)	2209, 2482
Bme1390I	CCNGG	8 cut(s)	417, 434, 493, 783, 1092, 1229, 1503, 2687
Bme18I	GGWCC	3 cut(s)	1126, 1280, 1308
BmeRI	GACNNNNNGTC	1 cut(s)	2700
BmgBI	CACGTC	2 cut(s)	274, 345
BmgT120I	GGNCC	4 cut(s)	1126, 1225, 1280, 1308
BmiI	GGNNCC	5 cut(s)	978, 1127, 1768, 2832, 2989
BmrFI	CCNGG	8 cut(s)	417, 434, 493, 783, 1092, 1229, 1503, 2687
BmrI	ACTGGG	1 cut(s)	2545
BmsI	GCATC	8 cut(s)	910, 1150, 1207, 1540, 2129, 2359, 2819, 3027
BmuI	ACTGGG	1 cut(s)	2545
BpiI	GAAGAC	5 cut(s)	1241, 1928, 2576, 2605, 2748
Bpu10I	CCTNAGC	1 cut(s)	924
Bpu14I	TTCGAA	2 cut(s)	218, 3010
BpuMI	CCSGG	4 cut(s)	417, 434, 493, 783
BsaBI	GATNNNNATC	2 cut(s)	1510, 1554
BsaHI	GRCGYC	2 cut(s)	108, 261
BsaJI	CCNNGG	6 cut(s)	433, 492, 1090, 1228, 1829, 2834
BsaWI	WCCGGW	2 cut(s)	589, 2452
BsaXI	ACNNNNNCTCC	4 cut(s)	293, 323, 363, 393
Bsc4I	CCNNNNNNNGG	8 cut(s)	94, 290, 416, 1021, 1096, 1369, 1474, 2626
Bse118I	RCCGGY	2 cut(s)	442, 1305
Bse1I	ACTGG	7 cut(s)	246, 739, 1332, 1468, 1768, 2540, 2620
Bse3DI	GCAATG	2 cut(s)	1083, 1579
Bse8I	GATNNNNATC	2 cut(s)	1510, 1554
BseAI	TCCGGA	1 cut(s)	589
BseBI	CCWGG	4 cut(s)	1092, 1229, 1503, 2687
BseDI	CCNNGG	6 cut(s)	433, 492, 1090, 1228, 1829, 2834
BseJI	GATNNNNATC	2 cut(s)	1510, 1554
BseLI	CCNNNNNNNGG	8 cut(s)	94, 290, 416, 1021, 1096, 1369, 1474, 2626
BseMI	GCAATG	2 cut(s)	1083, 1579
BseMII	CTCAG	5 cut(s)	915, 951, 1665, 2493, 2713
BseNI	ACTGG	7 cut(s)	246, 739, 1332, 1468, 1768, 2540, 2620
BseRI	GAGGAG	3 cut(s)	314, 1215, 2799
BseX3I	CGGCCG	1 cut(s)	440
BseXI	GCAGC	8 cut(s)	306, 973, 1711, 1714, 1717, 2256, 2722, 2819
BsgI	GTGCAG	2 cut(s)	367, 972
Bsh1236I	CGCG	2 cut(s)	212, 2715
Bsh1285I	CGRYCG	2 cut(s)	443, 2937
BshNI	GGYRCC	2 cut(s)	1766, 2987
BsiEI	CGRYCG	2 cut(s)	443, 2937
BsiHKAI	GWGCWC	2 cut(s)	27, 2392
BslFI	GGGAC	6 cut(s)	171, 450, 1139, 1656, 2328, 2707
BslI	CCNNNNNNNGG	8 cut(s)	94, 290, 416, 1021, 1096, 1369, 1474, 2626
BsmAI	GTCTC	3 cut(s)	366, 417, 2417
BsmBI	CGTCTC	2 cut(s)	366, 417
BsmFI	GGGAC	6 cut(s)	171, 450, 1139, 1656, 2328, 2707
BsmI	GAATGC	1 cut(s)	1150
Bsp119I	TTCGAA	2 cut(s)	218, 3010
Bsp1286I	GDGCHC	4 cut(s)	27, 88, 526, 2392
Bsp13I	TCCGGA	1 cut(s)	589
Bsp1407I	TGTACA	2 cut(s)	792, 850
Bsp19I	CCATGG	1 cut(s)	2834
BspCNI	CTCAG	5 cut(s)	916, 952, 1664, 2494, 2712
BspEI	TCCGGA	1 cut(s)	589
BspFNI	CGCG	2 cut(s)	212, 2715
BspLI	GGNNCC	5 cut(s)	978, 1127, 1768, 2832, 2989
BspMAI	CTGCAG	1 cut(s)	2649
BspPI	GGATC	6 cut(s)	193, 971, 984, 1033, 2825, 2838
BspT104I	TTCGAA	2 cut(s)	218, 3010
BspT107I	GGYRCC	2 cut(s)	1766, 2987
BspTI	CTTAAG	2 cut(s)	659, 680
BsrBI	CCGCTC	1 cut(s)	286
BsrDI	GCAATG	2 cut(s)	1083, 1579
BsrFI	RCCGGY	2 cut(s)	442, 1305
BsrGI	TGTACA	2 cut(s)	792, 850
BsrI	ACTGG	7 cut(s)	246, 739, 1332, 1468, 1768, 2540, 2620
BssAI	RCCGGY	2 cut(s)	442, 1305
BssECI	CCNNGG	6 cut(s)	433, 492, 1090, 1228, 1829, 2834
BssNI	GRCGYC	2 cut(s)	108, 261
BssSI	CACGAG	1 cut(s)	796
BssT1I	CCWWGG	2 cut(s)	1829, 2834
Bst2BI	CACGAG	1 cut(s)	796
Bst2UI	CCWGG	4 cut(s)	1092, 1229, 1503, 2687
Bst6I	CTCTTC	2 cut(s)	1089, 2459
BstACI	GRCGYC	2 cut(s)	108, 261
BstAFI	CTTAAG	2 cut(s)	659, 680
BstAPI	GCANNNNNTGC	1 cut(s)	2816
BstAUI	TGTACA	2 cut(s)	792, 850
BstBI	TTCGAA	2 cut(s)	218, 3010
BstC8I	GCNNGC	7 cut(s)	444, 1023, 1422, 1970, 2223, 2477, 3019
BstDEI	CTNAG	7 cut(s)	924, 960, 1561, 1629, 1651, 2502, 2699
BstDSI	CCRYGG	1 cut(s)	2834
BstFNI	CGCG	2 cut(s)	212, 2715
BstH2I	RGCGCY	1 cut(s)	808
BstHHI	GCGC	4 cut(s)	242, 807, 1184, 2715
BstMAI	GTCTC	3 cut(s)	366, 417, 2417
BstMCI	CGRYCG	2 cut(s)	443, 2937
BstNI	CCWGG	4 cut(s)	1092, 1229, 1503, 2687
BstNSI	RCATGY	3 cut(s)	857, 1342, 1420
BstSCI	CCNGG	8 cut(s)	415, 432, 491, 781, 1090, 1227, 1501, 2685
BstSFI	CTRYAG	2 cut(s)	2645, 2850
BstUI	CGCG	2 cut(s)	212, 2715
BstV1I	GCAGC	8 cut(s)	306, 973, 1711, 1714, 1717, 2256, 2722, 2819
BstV2I	GAAGAC	5 cut(s)	1241, 1928, 2576, 2605, 2748
BstX2I	RGATCY	4 cut(s)	976, 2209, 2482, 2830
BstYI	RGATCY	4 cut(s)	976, 2209, 2482, 2830
BstZI	CGGCCG	1 cut(s)	440
BtgI	CCRYGG	1 cut(s)	2834
BtgZI	GCGATG	3 cut(s)	270, 2378, 2864
BtrI	CACGTC	2 cut(s)	274, 345
BtsIMutI	CAGTG	5 cut(s)	1339, 1475, 2627, 2752, 2771
Cac8I	GCNNGC	7 cut(s)	444, 1023, 1422, 1970, 2223, 2477, 3019
CfoI	GCGC	4 cut(s)	242, 807, 1184, 2715
Cfr10I	RCCGGY	2 cut(s)	442, 1305
Cfr13I	GGNCC	4 cut(s)	1126, 1225, 1280, 1308
CseI	GACGC	2 cut(s)	1487, 1593
Csp6I	GTAC	4 cut(s)	793, 851, 1012, 1953
CviQI	GTAC	4 cut(s)	793, 851, 1012, 1953
DdeI	CTNAG	7 cut(s)	924, 960, 1561, 1629, 1651, 2502, 2699
DraI	TTTAAA	1 cut(s)	2059
DraIII	CACNNNGTG	1 cut(s)	2387
DriI	GACNNNNNGTC	1 cut(s)	2700
EaeI	YGGCCR	4 cut(s)	96, 430, 440, 1194
EagI	CGGCCG	1 cut(s)	440
Eam1104I	CTCTTC	2 cut(s)	1089, 2459
Eam1105I	GACNNNNNGTC	1 cut(s)	2700
EarI	CTCTTC	2 cut(s)	1089, 2459
EciI	GGCGGA	1 cut(s)	2443
Ecl136II	GAGCTC	1 cut(s)	25
EclXI	CGGCCG	1 cut(s)	440
Eco130I	CCWWGG	2 cut(s)	1829, 2834
Eco147I	AGGCCT	3 cut(s)	750, 1972, 2051
Eco24I	GRGCYC	3 cut(s)	27, 88, 526
Eco32I	GATATC	1 cut(s)	2652
Eco47I	GGWCC	3 cut(s)	1126, 1280, 1308
Eco52I	CGGCCG	1 cut(s)	440
Eco53kI	GAGCTC	1 cut(s)	25
Eco57I	CTGAAG	2 cut(s)	2599, 2795
EcoICRI	GAGCTC	1 cut(s)	25
EcoRI	GAATTC	5 cut(s)	192, 214, 1624, 2251, 2431
EcoRII	CCWGG	4 cut(s)	1090, 1227, 1501, 2685
EcoRV	GATATC	1 cut(s)	2652
EcoT14I	CCWWGG	2 cut(s)	1829, 2834
EcoT22I	ATGCAT	3 cut(s)	1064, 1150, 2122
EcoT38I	GRGCYC	3 cut(s)	27, 88, 526
ErhI	CCWWGG	2 cut(s)	1829, 2834
Esp3I	CGTCTC	2 cut(s)	366, 417
FaqI	GGGAC	6 cut(s)	171, 450, 1139, 1656, 2328, 2707
FauI	CCCGC	1 cut(s)	325
FauNDI	CATATG	3 cut(s)	1426, 1591, 2348
FbaI	TGATCA	3 cut(s)	1581, 2080, 2907
FblI	GTMKAC	3 cut(s)	341, 1540, 1804
FriOI	GRGCYC	3 cut(s)	27, 88, 526
FspBI	CTAG	7 cut(s)	746, 1046, 1211, 1463, 2226, 2421, 2636
GlaI	GCGC	4 cut(s)	241, 806, 1183, 2714
HaeII	RGCGCY	1 cut(s)	808
HgaI	GACGC	2 cut(s)	1487, 1593
HhaI	GCGC	4 cut(s)	242, 807, 1184, 2715
Hin1I	GRCGYC	2 cut(s)	108, 261
Hin6I	GCGC	4 cut(s)	240, 805, 1182, 2713
HinP1I	GCGC	4 cut(s)	240, 805, 1182, 2713
HincII	GTYRAC	3 cut(s)	342, 1295, 2705
HindII	GTYRAC	3 cut(s)	342, 1295, 2705
HindIII	AAGCTT	1 cut(s)	2219
Hpy99I	CGWCG	8 cut(s)	23, 53, 110, 266, 269, 346, 487, 1288
HpyCH4IV	ACGT	5 cut(s)	108, 261, 273, 344, 375
HpyF3I	CTNAG	7 cut(s)	924, 960, 1561, 1629, 1651, 2502, 2699
HpySE526I	ACGT	5 cut(s)	108, 261, 273, 344, 375
Hsp92I	GRCGYC	2 cut(s)	108, 261
HspAI	GCGC	4 cut(s)	240, 805, 1182, 2713
Kpn2I	TCCGGA	1 cut(s)	589
KroI	GCCGGC	1 cut(s)	442
KroNI	GCCGGC	1 cut(s)	444
Ksp22I	TGATCA	3 cut(s)	1581, 2080, 2907
LmnI	GCTCC	2 cut(s)	592, 1073
Lsp1109I	GCAGC	8 cut(s)	306, 973, 1711, 1714, 1717, 2256, 2722, 2819
LweI	GCATC	8 cut(s)	910, 1150, 1207, 1540, 2129, 2359, 2819, 3027
MaeI	CTAG	7 cut(s)	746, 1046, 1211, 1463, 2226, 2421, 2636
MaeII	ACGT	5 cut(s)	108, 261, 273, 344, 375
MaeIII	GTNAC	7 cut(s)	109, 785, 1334, 1372, 2666, 2747, 2769
MbiI	CCGCTC	1 cut(s)	286
MfeI	CAATTG	1 cut(s)	846
MflI	RGATCY	4 cut(s)	976, 2209, 2482, 2830
MhlI	GDGCHC	4 cut(s)	27, 88, 526, 2392
MlsI	TGGCCA	2 cut(s)	98, 1196
MluNI	TGGCCA	2 cut(s)	98, 1196
MlyI	GAGTC	4 cut(s)	1075, 2191, 2515, 2689
MmeI	TCCRAC	4 cut(s)	90, 928, 1066, 2770
Mox20I	TGGCCA	2 cut(s)	98, 1196
Mph1103I	ATGCAT	3 cut(s)	1064, 1150, 2122
MroI	TCCGGA	1 cut(s)	589
MroNI	GCCGGC	1 cut(s)	442
MroXI	GAANNNNTTC	2 cut(s)	2583, 2955
MscI	TGGCCA	2 cut(s)	98, 1196
MslI	CAYNNNNRTG	3 cut(s)	994, 2115, 2756
Msp20I	TGGCCA	2 cut(s)	98, 1196
MspA1I	CMGCKG	2 cut(s)	2849, 2974
MspCI	CTTAAG	2 cut(s)	659, 680
MspR9I	CCNGG	8 cut(s)	417, 434, 493, 783, 1092, 1229, 1503, 2687
MunI	CAATTG	1 cut(s)	846
Mva1269I	GAATGC	1 cut(s)	1150
MvaI	CCWGG	4 cut(s)	1092, 1229, 1503, 2687
MvnI	CGCG	2 cut(s)	212, 2715
NaeI	GCCGGC	1 cut(s)	444
NciI	CCSGG	4 cut(s)	417, 434, 493, 783
NcoI	CCATGG	1 cut(s)	2834
NdeI	CATATG	3 cut(s)	1426, 1591, 2348
NgoMIV	GCCGGC	1 cut(s)	442
NlaIV	GGNNCC	5 cut(s)	978, 1127, 1768, 2832, 2989
NmeAIII	GCCGAG	1 cut(s)	267
NmuCI	GTSAC	6 cut(s)	109, 785, 1334, 1372, 2747, 2769
NsiI	ATGCAT	3 cut(s)	1064, 1150, 2122
NspI	RCATGY	3 cut(s)	857, 1342, 1420
NspV	TTCGAA	2 cut(s)	218, 3010
OliI	CACNNNNGTG	1 cut(s)	994
PceI	AGGCCT	3 cut(s)	750, 1972, 2051
PciI	ACATGT	1 cut(s)	1338
PcsI	WCGNNNNNNNCGW	3 cut(s)	270, 294, 2401
PctI	GAATGC	1 cut(s)	1150
PdiI	GCCGGC	1 cut(s)	444
PdmI	GAANNNNTTC	2 cut(s)	2583, 2955
PfeI	GAWTC	6 cut(s)	53, 488, 1206, 1487, 2543, 2842
PflFI	GACNNNGTC	1 cut(s)	263
PfoI	TCCNGGA	1 cut(s)	1501
PleI	GAGTC	4 cut(s)	1075, 2190, 2514, 2689
PpsI	GAGTC	4 cut(s)	1075, 2190, 2514, 2689
PscI	ACATGT	1 cut(s)	1338
Psp124BI	GAGCTC	1 cut(s)	27
Psp6I	CCWGG	4 cut(s)	1090, 1227, 1501, 2685
PspGI	CCWGG	4 cut(s)	1090, 1227, 1501, 2685
PspN4I	GGNNCC	5 cut(s)	978, 1127, 1768, 2832, 2989
PspPI	GGNCC	4 cut(s)	1126, 1225, 1280, 1308
PstI	CTGCAG	1 cut(s)	2649
PsuI	RGATCY	4 cut(s)	976, 2209, 2482, 2830
PsyI	GACNNNGTC	1 cut(s)	263
PvuII	CAGCTG	1 cut(s)	2849
RsaI	GTAC	4 cut(s)	794, 852, 1013, 1954
RsaNI	GTAC	4 cut(s)	793, 851, 1012, 1953
RseI	CAYNNNNRTG	3 cut(s)	994, 2115, 2756
SacI	GAGCTC	1 cut(s)	27
SalI	GTCGAC	1 cut(s)	340
Sau96I	GGNCC	4 cut(s)	1126, 1225, 1280, 1308
SchI	GAGTC	4 cut(s)	1075, 2191, 2515, 2689
ScrFI	CCNGG	8 cut(s)	417, 434, 493, 783, 1092, 1229, 1503, 2687
SduI	GDGCHC	4 cut(s)	27, 88, 526, 2392
SfaNI	GCATC	8 cut(s)	910, 1150, 1207, 1540, 2129, 2359, 2819, 3027
SfcI	CTRYAG	2 cut(s)	2645, 2850
SfuI	TTCGAA	2 cut(s)	218, 3010
SinI	GGWCC	3 cut(s)	1126, 1280, 1308
SmiMI	CAYNNNNRTG	3 cut(s)	994, 2115, 2756
SmlI	CTYRAG	2 cut(s)	659, 680
SmoI	CTYRAG	2 cut(s)	659, 680
SseBI	AGGCCT	3 cut(s)	750, 1972, 2051
SspI	AATATT	1 cut(s)	2204
SspMI	CTAG	7 cut(s)	746, 1046, 1211, 1463, 2226, 2421, 2636
SstI	GAGCTC	1 cut(s)	27
StuI	AGGCCT	3 cut(s)	750, 1972, 2051
StyD4I	CCNGG	8 cut(s)	415, 432, 491, 781, 1090, 1227, 1501, 2685
StyI	CCWWGG	2 cut(s)	1829, 2834
TaiI	ACGT	5 cut(s)	111, 264, 276, 347, 378
TaqI	TCGA	7 cut(s)	51, 135, 218, 341, 2185, 2937, 3010
TatI	WGTACW	2 cut(s)	792, 850
TfiI	GAWTC	6 cut(s)	53, 488, 1206, 1487, 2543, 2842
TscAI	CASTG	5 cut(s)	1339, 1475, 2627, 2752, 2778
TseFI	GTSAC	6 cut(s)	109, 785, 1334, 1372, 2747, 2769
TseI	GCWGC	8 cut(s)	294, 986, 1724, 1727, 1730, 2244, 2710, 2807
Tsp45I	GTSAC	6 cut(s)	109, 785, 1334, 1372, 2747, 2769
TspDTI	ATGAA	7 cut(s)	707, 900, 1418, 2099, 2504, 2580, 2867
TspGWI	ACGGA	3 cut(s)	29, 500, 999
TspRI	CASTG	5 cut(s)	1339, 1475, 2627, 2752, 2778
Tth111I	GACNNNGTC	1 cut(s)	263
Vha464I	CTTAAG	2 cut(s)	659, 680
VpaK11BI	GGWCC	3 cut(s)	1126, 1280, 1308
XbaI	TCTAGA	1 cut(s)	2635
XceI	RCATGY	3 cut(s)	857, 1342, 1420
XmiI	GTMKAC	3 cut(s)	341, 1540, 1804
XmnI	GAANNNNTTC	2 cut(s)	2583, 2955
XspI	CTAG	7 cut(s)	746, 1046, 1211, 1463, 2226, 2421, 2636
ZraI	GACGTC	2 cut(s)	109, 262
Zsp2I	ATGCAT	3 cut(s)	1064, 1150, 2122

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.