Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002288

Physical Location & Seq

Genomic Coordinates Reverse (-)

82001519 .. 82007997

Gene Structure 6479 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022256

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2337 bp

ATGGCCCAATCCAACACTGCAATTGGACATAATCCGAGCCGTACACATGACCAACAACGACGTCACCGCCGCGATCAACATCATCTCCGACTCCCAAATCCAAAGAAAGATCGGATGTTGCGAGCCCTGAAGTCGCGAATTCGAAGCCGAATGACAGTGATTGCCCCGCCAGTTCAGGCGATGAAGTCGTCGGTGACGTGTAGAGGGAGGAGGATCAAGGCCGGAGGGAGACCAAGTGGATTTCACATTTCCTTTGAAGATTTGGCCGTCTCCCGGCAAGGTTTTCCGGAGAGGACTGCCGGCGGCGGCGTGTTCGGAGATTGTGAGGTTCTCCACGACCGATTCCGGAGAGATAACAAGGTTAAAATTGAGGGGCATTGTAAATCTGCTCCTGATGTCTTGAGCGTTTTGGACACCATTCTCTTGTGTATAAGTGTGTATATCAATAGTTCTATGTTCCTTAAGCAAAAGCAGACCTCGCTTAAGGCAGCCAGTAATTCCACGGAGGAAACAGTGGTTCATCCTCTCCCGACATTGTTCCAGTTGCTTGGCCTGACTCCTTTCCAGAAGGCAGAATTTACTCCTGGTGACTTGTACACGAGAAAGCGCCCTTTAGACCAAAAGGACAGTTCTGGCCTCTGTGCCTCAATTGTACATGCAAATAAGCATAAAAATCCTACTAGAAATGAAGATGAAGTTGAAAACAAGGAATGTACTTCGGATGCTGAGGTTGATAACATCGTTGGGGTTGGAGATAGCTCTGAGTTAGAGGAAATGGATCTTCCTGCTGCACTTCTGTGTGAACTTCGTCCGTACCAAAAGCAGGCTCTTCATTGGATGATCCAACTAGAAAAGGGAAAATGCATGGATGAGGGAGCAATTGACTCTTCACCCTTGTTGGGAGGCATATCGTCTTGCAGACAAAATTTCCAAGCGCACTTCAAATGGCCAGAGGAGGAGTATGTCTCAAGACATGAATCATCAGGTAGTCAACCTATGAGCCGGTCCTCCGGTGAAGATATTGAAGCCAGTGACATGTCAGACCATTCATCAAAACTCCCAAAGGCGGTCACAAAGTTTTCAGGATTTCATAAGTTGATGAAGCAAAAAAACACGCTTGCCTATGGGGGCAGTTTGATTGTATGTCCTATGACCCTTCTTGGCCAGTGGAAGAATTCTAAGGACAACGGGGGACTTCACTCAGTTAGTTGGTTTAGAGTGGTTCTTGATGAAGCACACACCATAAAATCCTCTAAAAGCCAAATCTCTACTGCTGCTGCTGCTTTAGTTACTGGTCGCCGATGGTGTCTTACTGGCACCCCCATACAGTTGAACAATCTAGAGTACTCTCTGCATGTTTATTTATATATTATATTTTGGTTAAAGCTGGTTCAAATAAACGTTTTCATCCAGAAACCCTTTGAGGAGGGTGATGAGAGAGGGTTAAAGCTGGTTCAATCTATCTTAAAGACGATTATGTTAAGGAGAACCAAGTTTAGCAATGATCGTGAAGGCAGGCATATTTTAGCCCTTCCACCTGCTGATATTCATGTGGTTTACTGTGAACTCACAGAAGCTGAAAAAGACTTTTATGAGGCCTTGTTTAAAAGATCAAAGGTGAAATTTGATCAATTTGTTGAGCAAGGATGGGTTCTTCATAATTATGCATCCATATTGGAATTACTTTTACGCCTTCGCCAATGTTGTGATCACCCATTTCTTGTGATGAGTCGAGGTGATACACAAGAATATTCAGATCTGAATAAGCTTGCAAGACGTTTTCTAAAAAGGCAGCCAGAATTCTGTGGAGGGGGAAGCCAAAGATTTGCCTTCACGGGCTTATTTGGCGGAATTCAACTTCTGGCTTATGTCCAGTTTGCAGGCTCAAAAAGCATTGTCTTCAGCCAGTGGACAGCCTTTCTGGACCTCCTGCAGATACCTCTTTCTCGGAGTAACATTCCATTTTTGCGCTTGGATGGGACTCTGAATCAACAGCAGCGCGAAAAAGTGTTAAAACAGTTTTCAGAAGACAGTGACATTCTGGTGTTGCTAATGTCACTGAAGGCCAGAGGAGTTGGAATAAATTTAACAGCAGCATCGAATGCCTTTGTCTTGGATCCATGGTGGAATCCAGCTGTAGAGGAACAAGCGGTCATGCGTATTCATCGCATCGGACAAACAAAAAGTGTGATGATCAAACGATTTATCGTGAAGGGAACAGTCGAGGAAAGAATGGAAGCAGTGCAAGCTCGTAAACATCGGTTAATTTCTGGCGCCTTGATTGACCAAGAAGTTCGTTCGGCTCGCATAGAAGAACTAAAGATGCCTTTTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009987 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044764 GO:0045003 GO:0046483 GO:0050896 GO:0051704 GO:0051716 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HIRAN Helicase_C SNF2_N zf-C3HC4_2 zf-C3HC4_3

Protein Analysis

779

Amino Acids

88.03

Weight (kDa)

9.24

Isoelectric Point (pI)

54.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SNF2-rel_dom	PF00176	398 - 573	1.2e-28	SNF2-related domain
Helicase_C	PF00271	627 - 725	3.7e-18	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002288)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22750
fragaria_vesca	FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660 FvH4_6g52660
malus_domestica	MD17G1018000.v1.1
prunus_persica	Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1 Prupe.3G300100_v2.0.a1
pyrus_communis	pycom17g01450
rosa_chinensis	RchiOBHm_Chr2g0174191 RchiOBHm_Chr4g0410751
rosa_laevigata	RLG00000022255 RLG00000022256 RLG00000029588
rosa_multiflora	Rmu_sc0000954.1_g000002 Rmu_ssc0000198.1_g000002
rosa_roxburghii	Rroxscaffold_2G00078340 Rroxscaffold_2G00078370 Rroxscaffold_5G00355500
rosa_rugosa	Rorug02G0575800 Rorug02G0575900 Rorug02G0576000 Rorug04G0376700
rosa_samantha	Rh2AG655200 Rh2BG666400 Rh2CG630600 Rh2DG681000 Rh4AG162800 Rh4BG161900 Rh4BG162000 Rh4DG158500 Rh7CG048600
rosa_wichuraiana	Rw2G053780 Rw4G013570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1546
AatII	GACGTC	1 cut(s)	64
Acc36I	ACCTGC	1 cut(s)	1546
AccB1I	GGYRCC	2 cut(s)	1316, 2273
AccII	CGCG	3 cut(s)	72, 136, 2001
AccIII	TCCGGA	2 cut(s)	286, 345
AciI	CCGC	8 cut(s)	67, 70, 167, 303, 306, 1067, 1848, 2150
AclI	AACGTT	1 cut(s)	1401
AclWI	GGATC	5 cut(s)	221, 786, 835, 2111, 2124
AcoI	YGGCCR	3 cut(s)	264, 947, 1162
AcsI	RAATTY	8 cut(s)	138, 575, 925, 1174, 1622, 1799, 1851, 2083
AcuI	CTGAAG	3 cut(s)	149, 1885, 2081
AcyI	GRCGYC	2 cut(s)	61, 2274
AfaI	GTAC	6 cut(s)	43, 596, 654, 715, 815, 1346
AfiI	CCNNNNNNNGG	4 cut(s)	273, 823, 899, 1066
AflII	CTTAAG	2 cut(s)	461, 482
AflIII	ACRYGT	2 cut(s)	197, 1035
AgsI	TTSAA	8 cut(s)	257, 701, 943, 1025, 1333, 1394, 1457, 1856
AjiI	CACGTC	1 cut(s)	198
AjnI	CCWGG	1 cut(s)	583
AleI	CACNNNNGTG	1 cut(s)	796
AluBI	AGCT	7 cut(s)	759, 1387, 1450, 1577, 1768, 2135, 2249
AluI	AGCT	7 cut(s)	759, 1387, 1450, 1577, 1768, 2135, 2249
Alw26I	GTCTC	3 cut(s)	223, 274, 970
AlwI	GGATC	5 cut(s)	221, 786, 835, 2111, 2124
AlwNI	CAGNNNCTG	1 cut(s)	1577
Aor13HI	TCCGGA	2 cut(s)	286, 345
AoxI	GGCC	9 cut(s)	3, 219, 264, 550, 634, 947, 1162, 1596, 2064
ApeKI	GCWGC	8 cut(s)	488, 788, 1274, 1277, 1280, 1792, 1996, 2093
ApoI	RAATTY	8 cut(s)	138, 575, 925, 1174, 1622, 1799, 1851, 2083
AspLEI	GCGC	5 cut(s)	609, 937, 1971, 2001, 2276
AspS9I	GGNCC	3 cut(s)	4, 1005, 1924
AsuC2I	CCSGG	1 cut(s)	274
AsuHPI	GGTGA	9 cut(s)	56, 205, 599, 882, 1025, 1442, 1630, 1703, 1748
AsuII	TTCGAA	1 cut(s)	142
AvaII	GGWCC	2 cut(s)	1005, 1924
BalI	TGGCCA	2 cut(s)	949, 1164
BamHI	GGATCC	1 cut(s)	2116
BanI	GGYRCC	2 cut(s)	1316, 2273
BanII	GRGCYC	1 cut(s)	127
BauI	CACGAG	1 cut(s)	598
BbsI	GAAGAC	2 cut(s)	1891, 2034
BbvCI	CCTCAGC	1 cut(s)	726
BbvI	GCAGC	8 cut(s)	500, 775, 1261, 1264, 1267, 1804, 2008, 2105
BccI	CCATC	3 cut(s)	1296, 1641, 1970
BceAI	ACGGC	2 cut(s)	24, 251
BciT130I	CCWGG	1 cut(s)	585
BclI	TGATCA	3 cut(s)	1627, 1708, 2193
BcnI	CCSGG	1 cut(s)	274
BcoDI	GTCTC	3 cut(s)	223, 274, 970
BfaI	CTAG	3 cut(s)	681, 848, 1340
BfmI	CTRYAG	2 cut(s)	1931, 2136
BfoI	RGCGCY	2 cut(s)	610, 2277
BfrI	CTTAAG	2 cut(s)	461, 482
BfuAI	ACCTGC	1 cut(s)	1546
BglII	AGATCT	1 cut(s)	1756
BmcAI	AGTACT	1 cut(s)	1346
Bme1390I	CCNGG	2 cut(s)	274, 585
Bme18I	GGWCC	2 cut(s)	1005, 1924
BmgBI	CACGTC	1 cut(s)	198
BmgT120I	GGNCC	3 cut(s)	4, 1005, 1924
BmiI	GGNNCC	3 cut(s)	1318, 2118, 2275
BmrFI	CCNGG	2 cut(s)	274, 585
BmsI	GCATC	5 cut(s)	712, 1676, 2105, 2178, 2313
BpiI	GAAGAC	2 cut(s)	1891, 2034
BplI	GAGNNNNNCTC	2 cut(s)	950, 982
Bpu10I	CCTNAGC	1 cut(s)	726
Bpu14I	TTCGAA	1 cut(s)	142
BpuEI	CTTGAG	2 cut(s)	421, 952
BpuMI	CCSGG	1 cut(s)	274
BsaBI	GATNNNNATC	1 cut(s)	78
BsaHI	GRCGYC	2 cut(s)	61, 2274
BsaI	GGTCTC	1 cut(s)	223
BsaJI	CCNNGG	2 cut(s)	501, 2120
BsaWI	WCCGGW	3 cut(s)	286, 345, 1010
BsaXI	ACNNNNNCTCC	4 cut(s)	69, 99, 220, 250
Bsc4I	CCNNNNNNNGG	4 cut(s)	273, 823, 899, 1066
Bse118I	RCCGGY	2 cut(s)	299, 1002
Bse1I	ACTGG	9 cut(s)	170, 492, 541, 1029, 1165, 1297, 1318, 1873, 1906
Bse3DI	GCAATG	1 cut(s)	1507
Bse8I	GATNNNNATC	1 cut(s)	78
BseAI	TCCGGA	2 cut(s)	286, 345
BseBI	CCWGG	1 cut(s)	585
BseDI	CCNNGG	2 cut(s)	501, 2120
BseGI	GGATG	9 cut(s)	120, 520, 727, 843, 874, 1407, 1652, 1667, 1981
BseJI	GATNNNNATC	1 cut(s)	78
BseLI	CCNNNNNNNGG	4 cut(s)	273, 823, 899, 1066
BseMI	GCAATG	1 cut(s)	1507
BseMII	CTCAG	3 cut(s)	717, 753, 1215
BseNI	ACTGG	9 cut(s)	170, 492, 541, 1029, 1165, 1297, 1318, 1873, 1906
BseRI	GAGGAG	5 cut(s)	223, 968, 971, 1439, 2085
BseXI	GCAGC	8 cut(s)	500, 775, 1261, 1264, 1267, 1804, 2008, 2105
BsgI	GTGCAG	1 cut(s)	774
Bsh1236I	CGCG	3 cut(s)	72, 136, 2001
Bsh1285I	CGRYCG	1 cut(s)	340
BshFI	GGCC	9 cut(s)	5, 221, 266, 552, 636, 949, 1164, 1598, 2066
BshNI	GGYRCC	2 cut(s)	1316, 2273
BsiEI	CGRYCG	1 cut(s)	340
BsiSI	CCGG	7 cut(s)	222, 274, 287, 300, 346, 1003, 1011
BslFI	GGGAC	2 cut(s)	1206, 1993
BslI	CCNNNNNNNGG	4 cut(s)	273, 823, 899, 1066
BsmAI	GTCTC	3 cut(s)	223, 274, 970
BsmBI	CGTCTC	1 cut(s)	274
BsmFI	GGGAC	2 cut(s)	1206, 1993
BsmI	GAATGC	1 cut(s)	2107
BsnI	GGCC	9 cut(s)	5, 221, 266, 552, 636, 949, 1164, 1598, 2066
Bso31I	GGTCTC	1 cut(s)	223
Bsp119I	TTCGAA	1 cut(s)	142
Bsp1286I	GDGCHC	1 cut(s)	127
Bsp13I	TCCGGA	2 cut(s)	286, 345
Bsp1407I	TGTACA	2 cut(s)	594, 652
Bsp19I	CCATGG	1 cut(s)	2120
Bsp68I	TCGCGA	1 cut(s)	136
BspACI	CCGC	8 cut(s)	67, 70, 167, 303, 306, 1067, 1848, 2150
BspANI	GGCC	9 cut(s)	5, 221, 266, 552, 636, 949, 1164, 1598, 2066
BspCNI	CTCAG	3 cut(s)	718, 754, 1214
BspEI	TCCGGA	2 cut(s)	286, 345
BspFNI	CGCG	3 cut(s)	72, 136, 2001
BspLI	GGNNCC	3 cut(s)	1318, 2118, 2275
BspMAI	CTGCAG	1 cut(s)	1935
BspMI	ACCTGC	1 cut(s)	1546
BspPI	GGATC	5 cut(s)	221, 786, 835, 2111, 2124
BspQI	GCTCTTC	1 cut(s)	834
BspT104I	TTCGAA	1 cut(s)	142
BspT107I	GGYRCC	2 cut(s)	1316, 2273
BspTI	CTTAAG	2 cut(s)	461, 482
BspTNI	GGTCTC	1 cut(s)	223
BsrDI	GCAATG	1 cut(s)	1507
BsrFI	RCCGGY	2 cut(s)	299, 1002
BsrGI	TGTACA	2 cut(s)	594, 652
BsrI	ACTGG	9 cut(s)	170, 492, 541, 1029, 1165, 1297, 1318, 1873, 1906
BssAI	RCCGGY	2 cut(s)	299, 1002
BssECI	CCNNGG	2 cut(s)	501, 2120
BssNI	GRCGYC	2 cut(s)	61, 2274
BssSI	CACGAG	1 cut(s)	598
BssT1I	CCWWGG	1 cut(s)	2120
Bst2BI	CACGAG	1 cut(s)	598
Bst2UI	CCWGG	1 cut(s)	585
Bst4CI	ACNGT	8 cut(s)	157, 514, 629, 1329, 1562, 2019, 2033, 2221
Bst6I	CTCTTC	2 cut(s)	834, 892
BstACI	GRCGYC	2 cut(s)	61, 2274
BstAFI	CTTAAG	2 cut(s)	461, 482
BstAPI	GCANNNNNTGC	1 cut(s)	2102
BstAUI	TGTACA	2 cut(s)	594, 652
BstBI	TTCGAA	1 cut(s)	142
BstC8I	GCNNGC	9 cut(s)	123, 301, 825, 1119, 1517, 1770, 1882, 2247, 2305
BstDEI	CTNAG	4 cut(s)	726, 762, 1179, 1201
BstDSI	CCRYGG	2 cut(s)	501, 2120
BstF5I	GGATG	9 cut(s)	120, 520, 727, 843, 874, 1407, 1652, 1667, 1981
BstFNI	CGCG	3 cut(s)	72, 136, 2001
BstH2I	RGCGCY	2 cut(s)	610, 2277
BstHHI	GCGC	5 cut(s)	609, 937, 1971, 2001, 2276
BstMAI	GTCTC	3 cut(s)	223, 274, 970
BstMCI	CGRYCG	1 cut(s)	340
BstMWI	GCNNNNNNNGC	6 cut(s)	478, 1280, 1845, 1890, 2102, 2246
BstNI	CCWGG	1 cut(s)	585
BstNSI	RCATGY	3 cut(s)	659, 1039, 1358
BstSCI	CCNGG	2 cut(s)	272, 583
BstSFI	CTRYAG	2 cut(s)	1931, 2136
BstUI	CGCG	3 cut(s)	72, 136, 2001
BstV1I	GCAGC	8 cut(s)	500, 775, 1261, 1264, 1267, 1804, 2008, 2105
BstV2I	GAAGAC	2 cut(s)	1891, 2034
BstX2I	RGATCY	3 cut(s)	778, 1756, 2116
BstXI	CCANNNNNNTGG	1 cut(s)	548
BstYI	RGATCY	3 cut(s)	778, 1756, 2116
BsuRI	GGCC	9 cut(s)	5, 221, 266, 552, 636, 949, 1164, 1598, 2066
BtgI	CCRYGG	2 cut(s)	501, 2120
BtgZI	GCGATG	2 cut(s)	194, 2150
BtrI	CACGTC	1 cut(s)	198
BtsCI	GGATG	9 cut(s)	120, 520, 727, 843, 874, 1407, 1652, 1667, 1981
BtsI	GCAGTG	2 cut(s)	15, 2247
BtsIMutI	CAGTG	9 cut(s)	15, 162, 519, 1036, 1172, 1913, 2038, 2057, 2247
BtuMI	TCGCGA	1 cut(s)	136
BveI	ACCTGC	1 cut(s)	1546
Cac8I	GCNNGC	9 cut(s)	123, 301, 825, 1119, 1517, 1770, 1882, 2247, 2305
CaiI	CAGNNNCTG	1 cut(s)	1577
CfoI	GCGC	5 cut(s)	609, 937, 1971, 2001, 2276
Cfr10I	RCCGGY	2 cut(s)	299, 1002
Cfr13I	GGNCC	3 cut(s)	4, 1005, 1924
Csp6I	GTAC	6 cut(s)	42, 595, 653, 714, 814, 1345
CviAII	CATG	9 cut(s)	47, 656, 865, 974, 1036, 1355, 1550, 2121, 2155
CviQI	GTAC	6 cut(s)	42, 595, 653, 714, 814, 1345
DdeI	CTNAG	4 cut(s)	726, 762, 1179, 1201
DinI	GGCGCC	1 cut(s)	2275
DraI	TTTAAA	1 cut(s)	1606
EaeI	YGGCCR	3 cut(s)	264, 947, 1162
Eam1104I	CTCTTC	2 cut(s)	834, 892
EarI	CTCTTC	2 cut(s)	834, 892
EciI	GGCGGA	1 cut(s)	1863
Eco130I	CCWWGG	1 cut(s)	2120
Eco147I	AGGCCT	1 cut(s)	1598
Eco24I	GRGCYC	1 cut(s)	127
Eco31I	GGTCTC	1 cut(s)	223
Eco47I	GGWCC	2 cut(s)	1005, 1924
Eco57I	CTGAAG	3 cut(s)	149, 1885, 2081
EcoRI	GAATTC	4 cut(s)	138, 1174, 1799, 1851
EcoRII	CCWGG	1 cut(s)	583
EcoT14I	CCWWGG	1 cut(s)	2120
EcoT22I	ATGCAT	2 cut(s)	866, 1669
EcoT38I	GRGCYC	1 cut(s)	127
EgeI	GGCGCC	1 cut(s)	2275
EheI	GGCGCC	1 cut(s)	2275
ErhI	CCWWGG	1 cut(s)	2120
Esp3I	CGTCTC	1 cut(s)	274
FaeI	CATG	9 cut(s)	50, 659, 868, 977, 1039, 1358, 1553, 2124, 2158
FaqI	GGGAC	2 cut(s)	1206, 1993
FatI	CATG	9 cut(s)	46, 655, 864, 973, 1035, 1354, 1549, 2120, 2154
FauI	CCCGC	1 cut(s)	174
FbaI	TGATCA	3 cut(s)	1627, 1708, 2193
FokI	GGATG	9 cut(s)	127, 507, 734, 850, 881, 1394, 1654, 1659, 1988
FriOI	GRGCYC	1 cut(s)	127
FspBI	CTAG	3 cut(s)	681, 848, 1340
GlaI	GCGC	5 cut(s)	608, 936, 1970, 2000, 2275
HaeII	RGCGCY	2 cut(s)	610, 2277
HaeIII	GGCC	9 cut(s)	5, 221, 266, 552, 636, 949, 1164, 1598, 2066
HapII	CCGG	7 cut(s)	222, 274, 287, 300, 346, 1003, 1011
HhaI	GCGC	5 cut(s)	609, 937, 1971, 2001, 2276
Hin1I	GRCGYC	2 cut(s)	61, 2274
Hin1II	CATG	9 cut(s)	50, 659, 868, 977, 1039, 1358, 1553, 2124, 2158
Hin6I	GCGC	5 cut(s)	607, 935, 1969, 1999, 2274
HinP1I	GCGC	5 cut(s)	607, 935, 1969, 1999, 2274
HincII	GTYRAC	1 cut(s)	992
HindII	GTYRAC	1 cut(s)	992
HindIII	AAGCTT	1 cut(s)	1766
HinfI	GANTC	9 cut(s)	90, 342, 556, 884, 977, 1729, 1981, 1987, 2128
HpaII	CCGG	7 cut(s)	222, 274, 287, 300, 346, 1003, 1011
HphI	GGTGA	9 cut(s)	56, 205, 599, 882, 1025, 1442, 1630, 1703, 1748
Hpy166II	GTNNAC	8 cut(s)	44, 597, 803, 992, 1558, 1565, 1911, 2255
Hpy8I	GTNNAC	8 cut(s)	44, 597, 803, 992, 1558, 1565, 1911, 2255
Hpy99I	CGWCG	2 cut(s)	63, 193
HpyAV	CCTTC	8 cut(s)	562, 1166, 1505, 1541, 1703, 1840, 2056, 2206
HpyCH4III	ACNGT	8 cut(s)	157, 514, 629, 1329, 1562, 2019, 2033, 2221
HpyCH4IV	ACGT	4 cut(s)	61, 197, 1401, 1777
HpyF10VI	GCNNNNNNNGC	6 cut(s)	478, 1280, 1845, 1890, 2102, 2246
HpyF3I	CTNAG	4 cut(s)	726, 762, 1179, 1201
HpySE526I	ACGT	4 cut(s)	61, 197, 1401, 1777
Hsp92I	GRCGYC	2 cut(s)	61, 2274
Hsp92II	CATG	9 cut(s)	50, 659, 868, 977, 1039, 1358, 1553, 2124, 2158
HspAI	GCGC	5 cut(s)	607, 935, 1969, 1999, 2274
KasI	GGCGCC	1 cut(s)	2273
Kpn2I	TCCGGA	2 cut(s)	286, 345
KroI	GCCGGC	1 cut(s)	299
KroNI	GCCGGC	1 cut(s)	301
Ksp22I	TGATCA	3 cut(s)	1627, 1708, 2193
LguI	GCTCTTC	1 cut(s)	834
LmnI	GCTCC	2 cut(s)	394, 875
Lsp1109I	GCAGC	8 cut(s)	500, 775, 1261, 1264, 1267, 1804, 2008, 2105
LweI	GCATC	5 cut(s)	712, 1676, 2105, 2178, 2313
MaeI	CTAG	3 cut(s)	681, 848, 1340
MaeII	ACGT	4 cut(s)	61, 197, 1401, 1777
MaeIII	GTNAC	9 cut(s)	62, 193, 587, 1031, 1069, 1288, 1952, 2033, 2055
MfeI	CAATTG	3 cut(s)	21, 648, 879
MflI	RGATCY	3 cut(s)	778, 1756, 2116
MhlI	GDGCHC	1 cut(s)	127
MlsI	TGGCCA	2 cut(s)	949, 1164
MluNI	TGGCCA	2 cut(s)	949, 1164
Mly113I	GGCGCC	1 cut(s)	2274
MlyI	GAGTC	5 cut(s)	84, 550, 878, 1738, 1975
MmeI	TCCRAC	5 cut(s)	36, 112, 730, 868, 2056
Mox20I	TGGCCA	2 cut(s)	949, 1164
Mph1103I	ATGCAT	2 cut(s)	866, 1669
MroI	TCCGGA	2 cut(s)	286, 345
MroNI	GCCGGC	1 cut(s)	299
MscI	TGGCCA	2 cut(s)	949, 1164
MslI	CAYNNNNRTG	3 cut(s)	796, 1662, 2042
Msp20I	TGGCCA	2 cut(s)	949, 1164
MspA1I	CMGCKG	1 cut(s)	2135
MspCI	CTTAAG	2 cut(s)	461, 482
MspI	CCGG	7 cut(s)	222, 274, 287, 300, 346, 1003, 1011
MspR9I	CCNGG	2 cut(s)	274, 585
MunI	CAATTG	3 cut(s)	21, 648, 879
Mva1269I	GAATGC	1 cut(s)	2107
MvaI	CCWGG	1 cut(s)	585
MvnI	CGCG	3 cut(s)	72, 136, 2001
MwoI	GCNNNNNNNGC	6 cut(s)	478, 1280, 1845, 1890, 2102, 2246
NaeI	GCCGGC	1 cut(s)	301
NarI	GGCGCC	1 cut(s)	2274
NciI	CCSGG	1 cut(s)	274
NcoI	CCATGG	1 cut(s)	2120
NgoMIV	GCCGGC	1 cut(s)	299
NlaIII	CATG	9 cut(s)	50, 659, 868, 977, 1039, 1358, 1553, 2124, 2158
NlaIV	GGNNCC	3 cut(s)	1318, 2118, 2275
NmuCI	GTSAC	7 cut(s)	62, 193, 587, 1031, 1069, 2033, 2055
NruI	TCGCGA	1 cut(s)	136
NsiI	ATGCAT	2 cut(s)	866, 1669
NspI	RCATGY	3 cut(s)	659, 1039, 1358
NspV	TTCGAA	1 cut(s)	142
OliI	CACNNNNGTG	1 cut(s)	796
PaqCI	CACCTGC	1 cut(s)	1546
PceI	AGGCCT	1 cut(s)	1598
PciI	ACATGT	1 cut(s)	1035
PciSI	GCTCTTC	1 cut(s)	834
PcsI	WCGNNNNNNNCGW	2 cut(s)	118, 194
PctI	GAATGC	1 cut(s)	2107
PdiI	GCCGGC	1 cut(s)	301
PfeI	GAWTC	4 cut(s)	342, 977, 1987, 2128
PleI	GAGTC	5 cut(s)	84, 550, 878, 1737, 1975
PluTI	GGCGCC	1 cut(s)	2277
PpsI	GAGTC	5 cut(s)	84, 550, 878, 1737, 1975
PscI	ACATGT	1 cut(s)	1035
Psp1406I	AACGTT	1 cut(s)	1401
Psp6I	CCWGG	1 cut(s)	583
PspGI	CCWGG	1 cut(s)	583
PspN4I	GGNNCC	3 cut(s)	1318, 2118, 2275
PspPI	GGNCC	3 cut(s)	4, 1005, 1924
PstI	CTGCAG	1 cut(s)	1935
PstNI	CAGNNNCTG	1 cut(s)	1577
PsuI	RGATCY	3 cut(s)	778, 1756, 2116
PvuII	CAGCTG	1 cut(s)	2135
RruI	TCGCGA	1 cut(s)	136
RsaI	GTAC	6 cut(s)	43, 596, 654, 715, 815, 1346
RsaNI	GTAC	6 cut(s)	42, 595, 653, 714, 814, 1345
RseI	CAYNNNNRTG	3 cut(s)	796, 1662, 2042
SapI	GCTCTTC	1 cut(s)	834
Sau96I	GGNCC	3 cut(s)	4, 1005, 1924
ScaI	AGTACT	1 cut(s)	1346
SchI	GAGTC	5 cut(s)	84, 550, 878, 1738, 1975
ScrFI	CCNGG	2 cut(s)	274, 585
SduI	GDGCHC	1 cut(s)	127
SfaNI	GCATC	5 cut(s)	712, 1676, 2105, 2178, 2313
SfcI	CTRYAG	2 cut(s)	1931, 2136
SfoI	GGCGCC	1 cut(s)	2275
SfuI	TTCGAA	1 cut(s)	142
SinI	GGWCC	2 cut(s)	1005, 1924
SmiMI	CAYNNNNRTG	3 cut(s)	796, 1662, 2042
SmlI	CTYRAG	4 cut(s)	400, 461, 482, 967
SmoI	CTYRAG	4 cut(s)	400, 461, 482, 967
SseBI	AGGCCT	1 cut(s)	1598
SsiI	CCGC	8 cut(s)	67, 70, 167, 303, 306, 1067, 1848, 2150
SspDI	GGCGCC	1 cut(s)	2273
SspI	AATATT	1 cut(s)	1751
SspMI	CTAG	3 cut(s)	681, 848, 1340
StuI	AGGCCT	1 cut(s)	1598
StyD4I	CCNGG	2 cut(s)	272, 583
StyI	CCWWGG	1 cut(s)	2120
TaaI	ACNGT	8 cut(s)	157, 514, 629, 1329, 1562, 2019, 2033, 2221
TaiI	ACGT	4 cut(s)	64, 200, 1404, 1780
TaqI	TCGA	4 cut(s)	142, 1732, 2099, 2223
TaqII	GACCGA	1 cut(s)	354
TatI	WGTACW	4 cut(s)	594, 652, 713, 1344
TauI	GCSGC	3 cut(s)	72, 306, 309
TfiI	GAWTC	4 cut(s)	342, 977, 1987, 2128
TscAI	CASTG	9 cut(s)	22, 162, 519, 1036, 1172, 1913, 2038, 2064, 2247
TseFI	GTSAC	7 cut(s)	62, 193, 587, 1031, 1069, 2033, 2055
TseI	GCWGC	8 cut(s)	488, 788, 1274, 1277, 1280, 1792, 1996, 2093
Tsp45I	GTSAC	7 cut(s)	62, 193, 587, 1031, 1069, 2033, 2055
TspGWI	ACGGA	2 cut(s)	518, 801
TspRI	CASTG	9 cut(s)	22, 162, 519, 1036, 1172, 1913, 2038, 2064, 2247
Vha464I	CTTAAG	2 cut(s)	461, 482
VpaK11BI	GGWCC	2 cut(s)	1005, 1924
XapI	RAATTY	8 cut(s)	138, 575, 925, 1174, 1622, 1799, 1851, 2083
XbaI	TCTAGA	1 cut(s)	1339
XceI	RCATGY	3 cut(s)	659, 1039, 1358
XspI	CTAG	3 cut(s)	681, 848, 1340
ZraI	GACGTC	1 cut(s)	62
ZrmI	AGTACT	1 cut(s)	1346
Zsp2I	ATGCAT	2 cut(s)	866, 1669

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.