AT5G35200

clathrin assembly protein At5g35200

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004817
Physical Location & Seq
Reverse (-)
13462155 .. 13466150
3996 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35200.1

Sequence Viewer

Length: 1635 bp
ATGTCAGGTGGTGGTGGATCACAGAGTAGTTTAAGGAGGTATCTTGGAGCTATTAAAGATACCACCACAGTTTCATTAGCTAAGGTCAATAGTGATTACAAGGAATTGGATATTGCTATTGTGAAGGCTACTAATCATGTGGAACGTCCTTCTAAAGAAAGATACATCAGAGCTATTTTTATGGCTATTTCAGCAACAAGGCCTCGTGCAGATGTGGCTTATTGTATCCATGCTCTTGCTAGGCGGTTATCAAGAACACATAACTGGGCGGTTGCACTGAAAACTCTTATTGTTATTCACCGTGCCTTGAGAGAAGTGGACCAGACATTTCATGAAGAAGTTATTAATTATAGCAGAAGCAGAAGTCATATGCTGAATATGTCTCATTTTAAAGATGATTCTGGTCCAAATGCTTGGGCTTATTCTGCTTGGGTCCGTTTCTATGCTCTATTCTTAGAGGAGCGATTAGAGTGTTTCCGTGTTTTGAAGTACGATGTTGAGGTTGACCCCCCAAGAACCAAAGATTTGGACACTCCTGATTTGCTCGAGCAACTTCCTGCTCTGCAAGAACTTCTTTTCCGTGTTCTTGACTGTCAGCCTGAAGGGGCAGCTGTTCAAAACCACATTATCCAATTGGCACTCTCAATGGTTATATCTGAGAGCACTAAAATATATCAAGCACTGACAGATGGCATAGACAATTTGGTTGATAAGTTCTTTGATATGCAACGGAATGATGCTGTGAAGGCTTTGGATATGTACAGACGAGCGGTAAAACAGGCAGGGAGGCTTTCTGAATTTTTCGAAGTTTGCAAAAGTGTTAATGTTGGACGTGGAGAGAGATTTATAAAGATTGAGCAGCCTCCCACTTCTTTTCTACAAGCAATGGAAGAGTATGTGAAAGAAGCACCATTAGCAGCAGGGGTCAAGAAAGAGCAGGTAGTGGAGAAACTTACTGCTCCCAAAGAGATTTTGGCCATAGAGTACGAAATTCCCCCGAAGGTTGTTGAAGAGAAACCAGCATCACCTGAACCAGTTAAAGCAGAAGCAGAGAAGCCTGTAGAGAAACAACCTGACCTTCTGTCTATGGATGATCCAGCTCCAATGGTCTCTGAACTTGAAGAGAAAAATGCTTTGGCTTTGGCTATTGTCCCTGTTAGCGTTGAACAGCCACATTCGACAACTGATTTTACAAATGGTAATTCGACTGGCTGGGAATTGGCACTTGTGACTGCCCCAAGCTCAAATGAAGGTGCCGCTGCAGATAGCAAATTGGCGGGTGGACTGGACAAGCTCACACTTGACAGCCTATATGAGGATGCAATCAGAGTGAGTCAACAGCAAAACAGGAGCTATAATCCATGGGAACAAAATCCAGTTCATAATGGTCACATGATGCACCAACCTTTCTATGCATCTAATGGAGTCGCAGCTCCTCAACCTTTTCAAATGGCGAACCAAAATCATCAAACATTCGGGTATCAGCATCAGAATGCAGGAATGATGATGGGACCTGTACAACAACCTTATCAACAACAGCAGCAGAACATGAACAATCCCTTTGGCAACCCATTTGTATCCAATGGTAATCCGCAACAACCGCAAGGTTACAACCCATATCCAAGGTATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

544

Amino Acids

61.18

Weight (kDa)

5.77

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 35 - 301 1e-89 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 848
Acc36I ACCTGC 1 cut(s) 928
AccB1I GGYRCC 1 cut(s) 1253
AccBSI CCGCTC 1 cut(s) 770
AciI CCGC 7 cut(s) 244, 269, 770, 1257, 1277, 1592, 1601
AclWI GGATC 2 cut(s) 25, 1088
AcoI YGGCCR 1 cut(s) 975
AcsI RAATTY 2 cut(s) 797, 990
AcuI CTGAAG 1 cut(s) 621
AfaI GTAC 4 cut(s) 491, 761, 986, 1518
AfiI CCNNNNNNNGG 1 cut(s) 1315
AgsI TTSAA 6 cut(s) 487, 617, 1010, 1121, 1166, 1448
AhdI GACNNNNNGTC 1 cut(s) 1081
AjiI CACGTC 1 cut(s) 833
AjuI GAANNNNNNNTTGG 2 cut(s) 1118, 1150
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
AluBI AGCT 9 cut(s) 50, 80, 173, 611, 1100, 1242, 1294, 1353, 1433
AluI AGCT 9 cut(s) 50, 80, 173, 611, 1100, 1242, 1294, 1353, 1433
Alw21I GWGCWC 1 cut(s) 665
Alw26I GTCTC 2 cut(s) 387, 1114
AlwI GGATC 2 cut(s) 25, 1088
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 2 cut(s) 200, 975
ApeKI GCWGC 6 cut(s) 608, 859, 917, 1259, 1430, 1540
ApoI RAATTY 2 cut(s) 797, 990
AseI ATTAAT 1 cut(s) 345
AspS9I GGNCC 4 cut(s) 319, 404, 433, 1511
AsuHPI GGTGA 2 cut(s) 290, 1017
AsuII TTCGAA 1 cut(s) 804
AvaI CYCGRG 1 cut(s) 545
AvaII GGWCC 4 cut(s) 319, 404, 433, 1511
BalI TGGCCA 1 cut(s) 977
BanI GGYRCC 1 cut(s) 1253
BauI CACGAG 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 665
BbvI GCAGC 6 cut(s) 620, 871, 929, 1246, 1442, 1552
BccI CCATC 2 cut(s) 683, 1501
BciVI GTATCC 2 cut(s) 236, 1588
BcoDI GTCTC 2 cut(s) 387, 1114
BfaI CTAG 1 cut(s) 240
BfmI CTRYAG 2 cut(s) 1059, 1260
BfuAI ACCTGC 1 cut(s) 928
BfuI GTATCC 2 cut(s) 236, 1588
BisI GCNGC 7 cut(s) 609, 860, 918, 1257, 1260, 1431, 1541
BlsI GCNGC 7 cut(s) 610, 861, 919, 1258, 1261, 1432, 1542
Bme18I GGWCC 4 cut(s) 319, 404, 433, 1511
BmeRI GACNNNNNGTC 1 cut(s) 1081
BmeT110I CYCGRG 1 cut(s) 545
BmgBI CACGTC 1 cut(s) 833
BmgT120I GGNCC 4 cut(s) 319, 404, 433, 1511
BmiI GGNNCC 3 cut(s) 434, 1255, 1512
BmrI ACTGGG 1 cut(s) 274
BmsI GCATC 6 cut(s) 727, 1031, 1309, 1386, 1424, 1495
BmuI ACTGGG 1 cut(s) 274
Bpu10I CCTNAGC 1 cut(s) 81
Bpu14I TTCGAA 1 cut(s) 804
BpuEI CTTGAG 1 cut(s) 328
BsaI GGTCTC 1 cut(s) 1114
BsaJI CCNNGG 2 cut(s) 1361, 1622
Bsc4I CCNNNNNNNGG 1 cut(s) 1315
Bse1I ACTGG 5 cut(s) 269, 1034, 1213, 1290, 1376
Bse3DI GCAATG 1 cut(s) 891
BseDI CCNNGG 2 cut(s) 1361, 1622
BseGI GGATG 2 cut(s) 1096, 1324
BseLI CCNNNNNNNGG 1 cut(s) 1315
BseMI GCAATG 1 cut(s) 891
BseMII CTCAG 1 cut(s) 648
BseNI ACTGG 5 cut(s) 269, 1034, 1213, 1290, 1376
BseRI GAGGAG 2 cut(s) 473, 1425
BseXI GCAGC 6 cut(s) 620, 871, 929, 1246, 1442, 1552
BseYI CCCAGC 1 cut(s) 1212
BsgI GTGCAG 1 cut(s) 228
BshFI GGCC 2 cut(s) 202, 977
BshNI GGYRCC 1 cut(s) 1253
BsiHKAI GWGCWC 1 cut(s) 665
BsiHKCI CYCGRG 1 cut(s) 545
BslFI GGGAC 2 cut(s) 1136, 1524
BslI CCNNNNNNNGG 1 cut(s) 1315
BsmAI GTCTC 2 cut(s) 387, 1114
BsmFI GGGAC 2 cut(s) 1136, 1524
BsmI GAATGC 1 cut(s) 1498
BsnI GGCC 2 cut(s) 202, 977
Bso31I GGTCTC 1 cut(s) 1114
BsoBI CYCGRG 1 cut(s) 545
Bsp119I TTCGAA 1 cut(s) 804
Bsp1286I GDGCHC 1 cut(s) 665
Bsp1407I TGTACA 2 cut(s) 759, 1516
Bsp143I GATC 2 cut(s) 17, 1093
Bsp19I CCATGG 1 cut(s) 1361
BspACI CCGC 7 cut(s) 244, 269, 770, 1257, 1277, 1592, 1601
BspANI GGCC 2 cut(s) 202, 977
BspCNI CTCAG 1 cut(s) 649
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 3 cut(s) 434, 1255, 1512
BspMAI CTGCAG 1 cut(s) 1264
BspMI ACCTGC 1 cut(s) 928
BspPI GGATC 2 cut(s) 25, 1088
BspT104I TTCGAA 1 cut(s) 804
BspT107I GGYRCC 1 cut(s) 1253
BspTNI GGTCTC 1 cut(s) 1114
BsrBI CCGCTC 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 891
BsrGI TGTACA 2 cut(s) 759, 1516
BsrI ACTGG 5 cut(s) 269, 1034, 1213, 1290, 1376
BssECI CCNNGG 2 cut(s) 1361, 1622
BssMI GATC 2 cut(s) 17, 1093
BssSI CACGAG 1 cut(s) 204
BssT1I CCWWGG 2 cut(s) 1361, 1622
Bst2BI CACGAG 1 cut(s) 204
Bst4CI ACNGT 3 cut(s) 70, 302, 593
Bst6I CTCTTC 3 cut(s) 885, 1005, 1116
BstAUI TGTACA 2 cut(s) 759, 1516
BstBI TTCGAA 1 cut(s) 804
BstDEI CTNAG 3 cut(s) 81, 454, 657
BstDSI CCRYGG 1 cut(s) 1361
BstENI CCTNNNNNAGG 1 cut(s) 1313
BstF5I GGATG 2 cut(s) 1096, 1324
BstKTI GATC 2 cut(s) 20, 1096
BstMAI GTCTC 2 cut(s) 387, 1114
BstMBI GATC 2 cut(s) 17, 1093
BstMWI GCNNNNNNNGC 6 cut(s) 191, 215, 425, 746, 914, 1600
BstSFI CTRYAG 2 cut(s) 1059, 1260
BstV1I GCAGC 6 cut(s) 620, 871, 929, 1246, 1442, 1552
BstXI CCANNNNNNTGG 2 cut(s) 414, 526
BsuI GTATCC 2 cut(s) 236, 1588
BsuRI GGCC 2 cut(s) 202, 977
BtgI CCRYGG 1 cut(s) 1361
BtrI CACGTC 1 cut(s) 833
BtsCI GGATG 2 cut(s) 1096, 1324
BtsIMutI CAGTG 2 cut(s) 275, 680
BveI ACCTGC 1 cut(s) 928
CciI TCATGA 1 cut(s) 331
Cfr13I GGNCC 4 cut(s) 319, 404, 433, 1511
Csp6I GTAC 4 cut(s) 490, 760, 985, 1517
CviAII CATG 6 cut(s) 137, 230, 332, 1362, 1393, 1549
CviQI GTAC 4 cut(s) 490, 760, 985, 1517
DdeI CTNAG 3 cut(s) 81, 454, 657
DpnI GATC 2 cut(s) 19, 1095
DpnII GATC 2 cut(s) 17, 1093
DraI TTTAAA 1 cut(s) 391
DriI GACNNNNNGTC 1 cut(s) 1081
EaeI YGGCCR 1 cut(s) 975
Eam1104I CTCTTC 3 cut(s) 885, 1005, 1116
Eam1105I GACNNNNNGTC 1 cut(s) 1081
EarI CTCTTC 3 cut(s) 885, 1005, 1116
Eco130I CCWWGG 2 cut(s) 1361, 1622
Eco147I AGGCCT 1 cut(s) 202
Eco31I GGTCTC 1 cut(s) 1114
Eco47I GGWCC 4 cut(s) 319, 404, 433, 1511
Eco57I CTGAAG 1 cut(s) 621
Eco88I CYCGRG 1 cut(s) 545
EcoNI CCTNNNNNAGG 1 cut(s) 1313
EcoO109I RGGNCCY 1 cut(s) 1511
EcoT14I CCWWGG 2 cut(s) 1361, 1622
EcoT22I ATGCAT 1 cut(s) 1417
ErhI CCWWGG 2 cut(s) 1361, 1622
FaeI CATG 6 cut(s) 140, 233, 335, 1365, 1396, 1552
FalI AAGNNNNNCTT 2 cut(s) 558, 590
FaqI GGGAC 2 cut(s) 1136, 1524
FatI CATG 6 cut(s) 136, 229, 331, 1361, 1392, 1548
FauI CCCGC 1 cut(s) 1270
FauNDI CATATG 1 cut(s) 369
Fnu4HI GCNGC 7 cut(s) 609, 860, 918, 1257, 1260, 1431, 1541
FokI GGATG 2 cut(s) 1103, 1331
Fsp4HI GCNGC 7 cut(s) 609, 860, 918, 1257, 1260, 1431, 1541
FspBI CTAG 1 cut(s) 240
GluI GCNGC 7 cut(s) 609, 860, 918, 1257, 1260, 1431, 1541
GsaI CCCAGC 1 cut(s) 1216
HaeIII GGCC 2 cut(s) 202, 977
Hin1II CATG 6 cut(s) 140, 233, 335, 1365, 1396, 1552
HincII GTYRAC 2 cut(s) 505, 1337
HindII GTYRAC 2 cut(s) 505, 1337
HinfI GANTC 3 cut(s) 398, 1333, 1425
HphI GGTGA 2 cut(s) 290, 1017
Hpy166II GTNNAC 4 cut(s) 319, 505, 1283, 1337
Hpy188I TCNGA 6 cut(s) 170, 658, 796, 1114, 1328, 1491
Hpy188III TCNNGA 5 cut(s) 252, 332, 536, 587, 928
Hpy8I GTNNAC 4 cut(s) 319, 505, 1283, 1337
HpyAV CCTTC 7 cut(s) 118, 159, 596, 739, 994, 1088, 1244
HpyCH4III ACNGT 3 cut(s) 70, 302, 593
HpyCH4IV ACGT 2 cut(s) 145, 832
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 215, 425, 746, 914, 1600
HpyF3I CTNAG 3 cut(s) 81, 454, 657
HpySE526I ACGT 2 cut(s) 145, 832
Hsp92II CATG 6 cut(s) 140, 233, 335, 1365, 1396, 1552
Kzo9I GATC 2 cut(s) 17, 1093
LmnI GCTCC 6 cut(s) 47, 460, 964, 1105, 1350, 1438
Lsp1109I GCAGC 6 cut(s) 620, 871, 929, 1246, 1442, 1552
LweI GCATC 6 cut(s) 727, 1031, 1309, 1386, 1424, 1495
MaeI CTAG 1 cut(s) 240
MaeII ACGT 2 cut(s) 145, 832
MaeIII GTNAC 3 cut(s) 1228, 1388, 1607
MalI GATC 2 cut(s) 19, 1095
MbiI CCGCTC 1 cut(s) 770
MboI GATC 2 cut(s) 17, 1093
MboII GAAGA 4 cut(s) 347, 902, 1022, 1133
MfeI CAATTG 1 cut(s) 632
MhlI GDGCHC 1 cut(s) 665
MlsI TGGCCA 1 cut(s) 977
MluCI AATT 9 cut(s) 104, 346, 632, 700, 797, 990, 1201, 1217, 1271
MluNI TGGCCA 1 cut(s) 977
MlyI GAGTC 2 cut(s) 1342, 1434
MmeI TCCRAC 1 cut(s) 808
MnlI CCTC 8 cut(s) 30, 213, 451, 493, 780, 873, 1309, 1446
Mox20I TGGCCA 1 cut(s) 977
Mph1103I ATGCAT 1 cut(s) 1417
MscI TGGCCA 1 cut(s) 977
MseI TTAA 6 cut(s) 32, 54, 345, 390, 822, 1038
MslI CAYNNNNRTG 1 cut(s) 1491
Msp20I TGGCCA 1 cut(s) 977
MspA1I CMGCKG 2 cut(s) 611, 1259
MunI CAATTG 1 cut(s) 632
Mva1269I GAATGC 1 cut(s) 1498
MwoI GCNNNNNNNGC 6 cut(s) 191, 215, 425, 746, 914, 1600
NcoI CCATGG 1 cut(s) 1361
NdeI CATATG 1 cut(s) 369
NdeII GATC 2 cut(s) 17, 1093
NlaIII CATG 6 cut(s) 140, 233, 335, 1365, 1396, 1552
NlaIV GGNNCC 3 cut(s) 434, 1255, 1512
NmuCI GTSAC 2 cut(s) 1228, 1388
NsiI ATGCAT 1 cut(s) 1417
NspV TTCGAA 1 cut(s) 804
PaeR7I CTCGAG 1 cut(s) 545
PagI TCATGA 1 cut(s) 331
PceI AGGCCT 1 cut(s) 202
PctI GAATGC 1 cut(s) 1498
PfeI GAWTC 1 cut(s) 398
PkrI GCNGC 7 cut(s) 610, 861, 919, 1258, 1261, 1432, 1542
PleI GAGTC 2 cut(s) 1341, 1433
PpsI GAGTC 2 cut(s) 1341, 1433
PpuMI RGGWCCY 1 cut(s) 1511
PshBI ATTAAT 1 cut(s) 345
PsiI TTATAA 1 cut(s) 848
Psp5II RGGWCCY 1 cut(s) 1511
PspFI CCCAGC 1 cut(s) 1212
PspN4I GGNNCC 3 cut(s) 434, 1255, 1512
PspPI GGNCC 4 cut(s) 319, 404, 433, 1511
PspPPI RGGWCCY 1 cut(s) 1511
PspXI VCTCGAGB 1 cut(s) 545
PstI CTGCAG 1 cut(s) 1264
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 4 cut(s) 491, 761, 986, 1518
RsaNI GTAC 4 cut(s) 490, 760, 985, 1517
RseI CAYNNNNRTG 1 cut(s) 1491
SaqAI TTAA 6 cut(s) 32, 54, 345, 390, 822, 1038
SatI GCNGC 7 cut(s) 609, 860, 918, 1257, 1260, 1431, 1541
Sau3AI GATC 2 cut(s) 17, 1093
Sau96I GGNCC 4 cut(s) 319, 404, 433, 1511
SchI GAGTC 2 cut(s) 1342, 1434
SduI GDGCHC 1 cut(s) 665
SfaNI GCATC 6 cut(s) 727, 1031, 1309, 1386, 1424, 1495
SfcI CTRYAG 2 cut(s) 1059, 1260
Sfr274I CTCGAG 1 cut(s) 545
SfuI TTCGAA 1 cut(s) 804
SinI GGWCC 4 cut(s) 319, 404, 433, 1511
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 1491
SmlI CTYRAG 2 cut(s) 307, 545
SmoI CTYRAG 2 cut(s) 307, 545
Sse9I AATT 9 cut(s) 104, 346, 632, 700, 797, 990, 1201, 1217, 1271
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 7 cut(s) 244, 269, 770, 1257, 1277, 1592, 1601
SspMI CTAG 1 cut(s) 240
StuI AGGCCT 1 cut(s) 202
StyI CCWWGG 2 cut(s) 1361, 1622
TaaI ACNGT 3 cut(s) 70, 302, 593
TaiI ACGT 2 cut(s) 148, 835
TaqI TCGA 4 cut(s) 546, 804, 1178, 1205
TasI AATT 9 cut(s) 104, 346, 632, 700, 797, 990, 1201, 1217, 1271
TatI WGTACW 2 cut(s) 759, 1516
TauI GCSGC 1 cut(s) 1259
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 6 cut(s) 32, 54, 345, 390, 822, 1038
Tru9I TTAA 6 cut(s) 32, 54, 345, 390, 822, 1038
TscAI CASTG 2 cut(s) 282, 687
TseFI GTSAC 2 cut(s) 1228, 1388
TseI GCWGC 6 cut(s) 608, 859, 917, 1259, 1430, 1540
Tsp45I GTSAC 2 cut(s) 1228, 1388
TspDTI ATGAA 6 cut(s) 63, 320, 348, 1263, 1370, 1565
TspGWI ACGGA 4 cut(s) 425, 467, 569, 745
TspRI CASTG 2 cut(s) 282, 687
VpaK11BI GGWCC 4 cut(s) 319, 404, 433, 1511
VspI ATTAAT 1 cut(s) 345
XagI CCTNNNNNAGG 1 cut(s) 1313
XapI RAATTY 2 cut(s) 797, 990
XcmI CCANNNNNNNNNTGG 1 cut(s) 970
XhoI CTCGAG 1 cut(s) 545
XspI CTAG 1 cut(s) 240
Zsp2I ATGCAT 1 cut(s) 1417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.