Rw2G002390

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004817
Physical Location & Seq
Forward (+)
1966949 .. 1972244
5296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G002390.1

Sequence Viewer

Length: 1626 bp
ATGGCAGGAGGGGGTGGCACGCAGAAGAGCATAAGAAAAGTACTTGGATCCCTCAAGGACACCACCACAGTTTCATTGGCGAAAGTCAATAGCGATTACAAGGAACTGGACATTGCGATAGTCAAGGCAACGAATCATGTTGAGCGTCCAGCCAAGGAAAAATACATCAGAGCTATATTCTCTGCTGTTTCTGCAACAAGACCTCGGGCTGATGTTGCATATTGCATCCATGGTCTTGCTAGGAGGTTAGCGAAGACACATAACTGGGCGGTTGCATTGAAAACTCTAATTGTTATTCATCGTGCTTTGAGGGAAGTGGACCCCACCTTTCATGAAGAACTCATGAATTATGGTAGAAGTAGAAACCATATGCTTAACTTGTCTCATTTCAAAGACGATTCCAGTCCAAATGCATGGGATTATTCAACCTGGATCCGTACCTATGCCTTGTTTTTGGAGGAGAGATTGGAGTGTTTTCGTGTGTTGAAATATGATGTTGAGATGGATCGCCCAAGGACCAAGGATTTGGACACTGCTGAATTGCTTGAGCATTTGCCTGCTCTGCAACAGCTTCTTTTTCGTGTTCTTGGTTGCCAGCCACAAGGGGCAGCAGTTCATAATTTTGTGGTCCAATTAGCACTTTCGATGGTTGCTTCAGAGAGCATCAAGATTTATCAAGCTATTAGTGACGGAACAGTCAATTTGGTTGACAAGTTCTTTGAGATGCAACGCCATGATGCTATGAAGGCTCTGGATATATATCGGAGGGCAGGACAGCAGGCAGAGAGACTGTCAGAGTTCTATGAAGTATGTAAGAGTCTTGATATTGGGCGAGGAGAAAAGTTTATTAAGATTGAGCAGCCCCCTGCATCATTTTTACAAGCCATGGAAGAGTACGTCAGAGAAGCTCCCCGTGTCTCAATAGTTCGCAAGGAGGTGATTGACAATAAAATTTCTGCCCCCAAGGAAATCTTGGCCATAGAGGACAAAAAGACCCAAGAGGATGAAGCACGTCCACCTTCCCCACCTCCACCTGAACCAGTAAAAGTGGTAGCACCTGTAGTTGAACCACCTGATTTGCTGGGTTTGAATGATCCTGTTCCAGATACTGCAGCATTAGATGAGAAGAATGCCATGGCTTTAGCTATTGTTCCAGTTTCTGATCAACCTGCGGCTCAACCTCAAGCAAATGGAACAGGTTGGGAATTGGCACTTGTTACAGCCCCAAGCTCAAATGAGAGTGCTACAGCTGCTAGCAAACTGGCGGGAGGGTTGGACTTGCTTACACTAGACAGCCTATACGATGATGCAATTAGAAGAAACAATCAAAATGTAAGCTACAACCCATGGGAGCAGGCTCCCATGAATGGTGGCATGATGCAACAACCAATTCATGATCCCTTTTATGCATCCAACACAGTGGCCGCACCACCCTCTGTACAGATGGCCGCAATGGCCAACCAGCAGCAACAGGCCTTCATGTATCAACAACAGCAGATGATGATGATGGGCCAACAACAACCGCAATCTTTGAATCCTTTTGGGAATCCTCATGGAGCACCTGCCCACCCCTATGGAGCAGGTATGCCTGTCCAAGCATACAATCCATACACGGGCCTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

541

Amino Acids

60.07

Weight (kDa)

5.95

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 35 - 301 4.6e-87 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1570
AasI GACNNNNNNGTC 1 cut(s) 695
Acc36I ACCTGC 3 cut(s) 1177, 1570, 1571
AciI CCGC 6 cut(s) 269, 1172, 1265, 1425, 1449, 1523
AclWI GGATC 7 cut(s) 42, 55, 427, 440, 513, 1088, 1391
AcoI YGGCCR 4 cut(s) 975, 1422, 1446, 1455
AcsI RAATTY 1 cut(s) 951
AcuI CTGAAG 1 cut(s) 639
AfaI GTAC 4 cut(s) 42, 439, 896, 1440
AfiI CCNNNNNNNGG 2 cut(s) 1367, 1613
AgsI TTSAA 7 cut(s) 280, 391, 426, 487, 1067, 1090, 1534
AjiI CACGTC 1 cut(s) 1013
AjnI CCWGG 1 cut(s) 428
AluBI AGCT 8 cut(s) 173, 571, 680, 908, 1145, 1230, 1250, 1338
AluI AGCT 8 cut(s) 173, 571, 680, 908, 1145, 1230, 1250, 1338
Alw21I GWGCWC 1 cut(s) 1561
Alw26I GTCTC 3 cut(s) 387, 781, 922
AlwI GGATC 7 cut(s) 42, 55, 427, 440, 513, 1088, 1391
AlwNI CAGNNNCTG 2 cut(s) 1109, 1160
Ama87I CYCGRG 1 cut(s) 204
AoxI GGCC 7 cut(s) 975, 1422, 1446, 1455, 1473, 1510, 1615
ApeKI GCWGC 5 cut(s) 608, 859, 1112, 1250, 1465
ApoI RAATTY 1 cut(s) 951
ArsI GACNNNNNNTTYG 4 cut(s) 508, 540, 701, 733
AspS9I GGNCC 5 cut(s) 319, 516, 628, 1510, 1615
AsuHPI GGTGA 1 cut(s) 949
AsuNHI GCTAGC 1 cut(s) 1253
AvaI CYCGRG 1 cut(s) 204
AvaII GGWCC 3 cut(s) 319, 516, 628
BalI TGGCCA 2 cut(s) 977, 1457
BamHI GGATCC 2 cut(s) 47, 432
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 1561
BbvI GCAGC 5 cut(s) 620, 871, 1124, 1237, 1477
BccI CCATC 4 cut(s) 496, 640, 1438, 1501
BciT130I CCWGG 1 cut(s) 430
BclI TGATCA 1 cut(s) 1162
BcoDI GTCTC 3 cut(s) 387, 781, 922
BfaI CTAG 3 cut(s) 240, 1254, 1289
BfmI CTRYAG 3 cut(s) 1059, 1110, 1245
BfuAI ACCTGC 3 cut(s) 1177, 1570, 1571
BglI GCCNNNNNGGC 1 cut(s) 1454
BisI GCNGC 8 cut(s) 609, 860, 1113, 1173, 1251, 1425, 1449, 1466
BlsI GCNGC 8 cut(s) 610, 861, 1114, 1174, 1252, 1426, 1450, 1467
BmcAI AGTACT 1 cut(s) 42
Bme1390I CCNGG 1 cut(s) 430
Bme18I GGWCC 3 cut(s) 319, 516, 628
BmeT110I CYCGRG 1 cut(s) 204
BmgBI CACGTC 1 cut(s) 1013
BmgT120I GGNCC 5 cut(s) 319, 516, 628, 1510, 1615
BmiI GGNNCC 4 cut(s) 49, 321, 434, 1359
BmrFI CCNGG 1 cut(s) 430
BmrI ACTGGG 1 cut(s) 274
BmsI GCATC 8 cut(s) 234, 672, 714, 727, 878, 1297, 1368, 1418
BmtI GCTAGC 1 cut(s) 1257
BmuI ACTGGG 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 260
BpuEI CTTGAG 3 cut(s) 38, 566, 1167
BsaBI GATNNNNATC 1 cut(s) 759
BsaJI CCNNGG 9 cut(s) 153, 203, 229, 512, 519, 885, 963, 1134, 1346
Bsc4I CCNNNNNNNGG 2 cut(s) 1367, 1613
Bse1I ACTGG 6 cut(s) 111, 269, 402, 1040, 1154, 1266
Bse3DI GCAATG 2 cut(s) 111, 1458
Bse8I GATNNNNATC 1 cut(s) 759
BseBI CCWGG 1 cut(s) 430
BseDI CCNNGG 9 cut(s) 153, 203, 229, 512, 519, 885, 963, 1134, 1346
BseGI GGATG 3 cut(s) 225, 1009, 1409
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 2 cut(s) 1367, 1613
BseMI GCAATG 2 cut(s) 111, 1458
BseNI ACTGG 6 cut(s) 111, 269, 402, 1040, 1154, 1266
BseRI GAGGAG 2 cut(s) 473, 849
BseXI GCAGC 5 cut(s) 620, 871, 1124, 1237, 1477
BseYI CCCAGC 1 cut(s) 1081
BshFI GGCC 7 cut(s) 977, 1424, 1448, 1457, 1475, 1512, 1617
BsiHKAI GWGCWC 1 cut(s) 1561
BsiHKCI CYCGRG 1 cut(s) 204
BslI CCNNNNNNNGG 2 cut(s) 1367, 1613
BsmAI GTCTC 3 cut(s) 387, 781, 922
BsmI GAATGC 1 cut(s) 1135
BsnI GGCC 7 cut(s) 977, 1424, 1448, 1457, 1475, 1512, 1617
BsoBI CYCGRG 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 1561
Bsp1407I TGTACA 1 cut(s) 1438
Bsp143I GATC 6 cut(s) 47, 432, 505, 1093, 1162, 1396
Bsp19I CCATGG 4 cut(s) 229, 885, 1134, 1346
BspACI CCGC 6 cut(s) 269, 1172, 1265, 1425, 1449, 1523
BspANI GGCC 7 cut(s) 977, 1424, 1448, 1457, 1475, 1512, 1617
BspHI TCATGA 3 cut(s) 331, 342, 1393
BspLI GGNNCC 4 cut(s) 49, 321, 434, 1359
BspMAI CTGCAG 1 cut(s) 1114
BspMI ACCTGC 3 cut(s) 1177, 1570, 1571
BspOI GCTAGC 1 cut(s) 1257
BspPI GGATC 7 cut(s) 42, 55, 427, 440, 513, 1088, 1391
BspQI GCTCTTC 1 cut(s) 20
BsrDI GCAATG 2 cut(s) 111, 1458
BsrGI TGTACA 1 cut(s) 1438
BsrI ACTGG 6 cut(s) 111, 269, 402, 1040, 1154, 1266
BssECI CCNNGG 9 cut(s) 153, 203, 229, 512, 519, 885, 963, 1134, 1346
BssMI GATC 6 cut(s) 47, 432, 505, 1093, 1162, 1396
BssT1I CCWWGG 8 cut(s) 153, 229, 512, 519, 885, 963, 1134, 1346
Bst2UI CCWGG 1 cut(s) 430
Bst4CI ACNGT 4 cut(s) 70, 697, 792, 1420
Bst6I CTCTTC 2 cut(s) 20, 885
BstAUI TGTACA 1 cut(s) 1438
BstC8I GCNNGC 6 cut(s) 20, 558, 596, 780, 1255, 1356
BstDSI CCRYGG 4 cut(s) 229, 885, 1134, 1346
BstF5I GGATG 3 cut(s) 225, 1009, 1409
BstKTI GATC 6 cut(s) 50, 435, 508, 1096, 1165, 1399
BstMAI GTCTC 3 cut(s) 387, 781, 922
BstMBI GATC 6 cut(s) 47, 432, 505, 1093, 1162, 1396
BstMWI GCNNNNNNNGC 6 cut(s) 191, 215, 562, 746, 1250, 1454
BstNI CCWGG 1 cut(s) 430
BstSCI CCNGG 1 cut(s) 428
BstSFI CTRYAG 3 cut(s) 1059, 1110, 1245
BstV1I GCAGC 5 cut(s) 620, 871, 1124, 1237, 1477
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 2 cut(s) 47, 432
BstXI CCANNNNNNTGG 4 cut(s) 414, 526, 1420, 1574
BstYI RGATCY 2 cut(s) 47, 432
BsuRI GGCC 7 cut(s) 977, 1424, 1448, 1457, 1475, 1512, 1617
BtgI CCRYGG 4 cut(s) 229, 885, 1134, 1346
BtrI CACGTC 1 cut(s) 1013
BtsCI GGATG 3 cut(s) 225, 1009, 1409
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 2 cut(s) 531, 1425
BveI ACCTGC 3 cut(s) 1177, 1570, 1571
Cac8I GCNNGC 6 cut(s) 20, 558, 596, 780, 1255, 1356
CaiI CAGNNNCTG 2 cut(s) 1109, 1160
CciI TCATGA 3 cut(s) 331, 342, 1393
Cfr13I GGNCC 5 cut(s) 319, 516, 628, 1510, 1615
CseI GACGC 1 cut(s) 134
Csp6I GTAC 4 cut(s) 41, 438, 895, 1439
CspCI CAANNNNNGTGG 2 cut(s) 1059, 1094
CviQI GTAC 4 cut(s) 41, 438, 895, 1439
DpnI GATC 6 cut(s) 49, 434, 507, 1095, 1164, 1398
DpnII GATC 6 cut(s) 47, 432, 505, 1093, 1162, 1396
DrdI GACNNNNNNGTC 1 cut(s) 695
DseDI GACNNNNNNGTC 1 cut(s) 695
EaeI YGGCCR 4 cut(s) 975, 1422, 1446, 1455
Eam1104I CTCTTC 2 cut(s) 20, 885
EarI CTCTTC 2 cut(s) 20, 885
Eco130I CCWWGG 8 cut(s) 153, 229, 512, 519, 885, 963, 1134, 1346
Eco147I AGGCCT 1 cut(s) 1475
Eco47I GGWCC 3 cut(s) 319, 516, 628
Eco57I CTGAAG 1 cut(s) 639
Eco88I CYCGRG 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 428
EcoT14I CCWWGG 8 cut(s) 153, 229, 512, 519, 885, 963, 1134, 1346
EcoT22I ATGCAT 2 cut(s) 415, 1411
ErhI CCWWGG 8 cut(s) 153, 229, 512, 519, 885, 963, 1134, 1346
FalI AAGNNNNNCTT 2 cut(s) 956, 988
FauI CCCGC 1 cut(s) 1258
FauNDI CATATG 1 cut(s) 369
FbaI TGATCA 1 cut(s) 1162
Fnu4HI GCNGC 8 cut(s) 609, 860, 1113, 1173, 1251, 1425, 1449, 1466
FokI GGATG 3 cut(s) 212, 1016, 1396
Fsp4HI GCNGC 8 cut(s) 609, 860, 1113, 1173, 1251, 1425, 1449, 1466
FspBI CTAG 3 cut(s) 240, 1254, 1289
GluI GCNGC 8 cut(s) 609, 860, 1113, 1173, 1251, 1425, 1449, 1466
GsaI CCCAGC 1 cut(s) 1085
HaeIII GGCC 7 cut(s) 977, 1424, 1448, 1457, 1475, 1512, 1617
HgaI GACGC 1 cut(s) 134
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HinfI GANTC 5 cut(s) 133, 398, 817, 1534, 1546
HphI GGTGA 1 cut(s) 949
Hpy166II GTNNAC 3 cut(s) 319, 709, 1016
Hpy188I TCNGA 6 cut(s) 170, 658, 765, 796, 902, 1162
Hpy188III TCNNGA 7 cut(s) 332, 343, 667, 752, 821, 1103, 1394
Hpy8I GTNNAC 3 cut(s) 319, 709, 1016
HpyAV CCTTC 3 cut(s) 739, 1029, 1486
HpyCH4III ACNGT 4 cut(s) 70, 697, 792, 1420
HpyCH4IV ACGT 2 cut(s) 897, 1012
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 215, 562, 746, 1250, 1454
HpySE526I ACGT 2 cut(s) 897, 1012
Ksp22I TGATCA 1 cut(s) 1162
Kzo9I GATC 6 cut(s) 47, 432, 505, 1093, 1162, 1396
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 5 cut(s) 913, 1351, 1363, 1556, 1577
Lsp1109I GCAGC 5 cut(s) 620, 871, 1124, 1237, 1477
LweI GCATC 8 cut(s) 234, 672, 714, 727, 878, 1297, 1368, 1418
MaeI CTAG 3 cut(s) 240, 1254, 1289
MaeII ACGT 2 cut(s) 897, 1012
MaeIII GTNAC 2 cut(s) 686, 1216
MalI GATC 6 cut(s) 49, 434, 507, 1095, 1164, 1398
MboI GATC 6 cut(s) 47, 432, 505, 1093, 1162, 1396
MboII GAAGA 6 cut(s) 37, 265, 347, 902, 1138, 1329
MflI RGATCY 2 cut(s) 47, 432
MhlI GDGCHC 1 cut(s) 1561
MlsI TGGCCA 2 cut(s) 977, 1457
MluNI TGGCCA 2 cut(s) 977, 1457
MlyI GAGTC 1 cut(s) 826
MmeI TCCRAC 2 cut(s) 1254, 1437
Mox20I TGGCCA 2 cut(s) 977, 1457
Mph1103I ATGCAT 2 cut(s) 415, 1411
MscI TGGCCA 2 cut(s) 977, 1457
MseI TTAA 2 cut(s) 375, 849
MslI CAYNNNNRTG 1 cut(s) 1572
Msp20I TGGCCA 2 cut(s) 977, 1457
MspA1I CMGCKG 1 cut(s) 1250
MspR9I CCNGG 1 cut(s) 430
Mva1269I GAATGC 1 cut(s) 1135
MvaI CCWGG 1 cut(s) 430
MwoI GCNNNNNNNGC 6 cut(s) 191, 215, 562, 746, 1250, 1454
NcoI CCATGG 4 cut(s) 229, 885, 1134, 1346
NdeI CATATG 1 cut(s) 369
NdeII GATC 6 cut(s) 47, 432, 505, 1093, 1162, 1396
NheI GCTAGC 1 cut(s) 1253
NlaIV GGNNCC 4 cut(s) 49, 321, 434, 1359
NmuCI GTSAC 1 cut(s) 686
NsiI ATGCAT 2 cut(s) 415, 1411
PagI TCATGA 3 cut(s) 331, 342, 1393
PaqCI CACCTGC 1 cut(s) 1570
PceI AGGCCT 1 cut(s) 1475
PciSI GCTCTTC 1 cut(s) 20
PctI GAATGC 1 cut(s) 1135
PfeI GAWTC 4 cut(s) 133, 398, 1534, 1546
PkrI GCNGC 8 cut(s) 610, 861, 1114, 1174, 1252, 1426, 1450, 1467
PleI GAGTC 1 cut(s) 825
PpsI GAGTC 1 cut(s) 825
Psp6I CCWGG 1 cut(s) 428
PspFI CCCAGC 1 cut(s) 1081
PspGI CCWGG 1 cut(s) 428
PspN4I GGNNCC 4 cut(s) 49, 321, 434, 1359
PspPI GGNCC 5 cut(s) 319, 516, 628, 1510, 1615
PstI CTGCAG 1 cut(s) 1114
PstNI CAGNNNCTG 2 cut(s) 1109, 1160
PsuI RGATCY 2 cut(s) 47, 432
PvuII CAGCTG 1 cut(s) 1250
RsaI GTAC 4 cut(s) 42, 439, 896, 1440
RsaNI GTAC 4 cut(s) 41, 438, 895, 1439
RseI CAYNNNNRTG 1 cut(s) 1572
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 2 cut(s) 375, 849
SatI GCNGC 8 cut(s) 609, 860, 1113, 1173, 1251, 1425, 1449, 1466
Sau3AI GATC 6 cut(s) 47, 432, 505, 1093, 1162, 1396
Sau96I GGNCC 5 cut(s) 319, 516, 628, 1510, 1615
ScaI AGTACT 1 cut(s) 42
SchI GAGTC 1 cut(s) 826
ScrFI CCNGG 1 cut(s) 430
SduI GDGCHC 1 cut(s) 1561
SfaNI GCATC 8 cut(s) 234, 672, 714, 727, 878, 1297, 1368, 1418
SfcI CTRYAG 3 cut(s) 1059, 1110, 1245
SfiI GGCCNNNNNGGCC 1 cut(s) 1454
SinI GGWCC 3 cut(s) 319, 516, 628
SmiMI CAYNNNNRTG 1 cut(s) 1572
SmlI CTYRAG 3 cut(s) 53, 545, 1182
SmoI CTYRAG 3 cut(s) 53, 545, 1182
SseBI AGGCCT 1 cut(s) 1475
SsiI CCGC 6 cut(s) 269, 1172, 1265, 1425, 1449, 1523
SspMI CTAG 3 cut(s) 240, 1254, 1289
StuI AGGCCT 1 cut(s) 1475
StyD4I CCNGG 1 cut(s) 428
StyI CCWWGG 8 cut(s) 153, 229, 512, 519, 885, 963, 1134, 1346
TaaI ACNGT 4 cut(s) 70, 697, 792, 1420
TaiI ACGT 2 cut(s) 900, 1015
TaqI TCGA 1 cut(s) 644
TatI WGTACW 2 cut(s) 40, 1438
TauI GCSGC 3 cut(s) 1175, 1427, 1451
TfiI GAWTC 4 cut(s) 133, 398, 1534, 1546
Tru1I TTAA 2 cut(s) 375, 849
Tru9I TTAA 2 cut(s) 375, 849
TscAI CASTG 2 cut(s) 538, 1425
TseFI GTSAC 1 cut(s) 686
TseI GCWGC 5 cut(s) 608, 859, 1112, 1250, 1465
Tsp45I GTSAC 1 cut(s) 686
TspGWI ACGGA 2 cut(s) 425, 705
TspRI CASTG 2 cut(s) 538, 1425
VpaK11BI GGWCC 3 cut(s) 319, 516, 628
XapI RAATTY 1 cut(s) 951
XcmI CCANNNNNNNNNTGG 2 cut(s) 73, 970
XspI CTAG 3 cut(s) 240, 1254, 1289
ZrmI AGTACT 1 cut(s) 42
Zsp2I ATGCAT 2 cut(s) 415, 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.