FvH4_1g02540

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004817
Physical Location & Seq
Forward (+)
1425254 .. 1430407
5154 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02540.t1

Sequence Viewer

Length: 1632 bp
ATGTCAGGAGGGGGTGGTACGCAGAAGAGCTTAAGAAAAGTGCTTGGATCCCTCAAGGACACCACCACAGTTTCGTTGGCGAAAGTCAATAGTGATTACAAGGAACTGGACATTGCGATAGTCAAGGCAACTAATCATGTTGAGCGTCCGGCCAAGGAGAAATACATCAGAGCTATTTTCTCTGCTGTTTCTGCAACAAGACCTCGGGCTGATGTTGCATATTGCATCCACGGTCTTGCTAGGAGGTTAGCAAAGACACATAACTGGGCGGTTGCATTGAAAACTCTGATTGTTATTCATCGTGCTTTGAGGGAAGTGGACCCTACCTTTCATGAAGAACTCATGAATTATGGTAGAAGTAGAAACCATATGCTTAACTTGTCTCATTTCAAAGACGATTCGAGTCCAAATGCATGGGATTATTCAACCTGGATCCGTACTTATGCGTTGTTTTTGGAGGAGAGATTGGAGTGTTGTCGTGTGTTGAAATATGATGTTGAGATGGATCGCCCAAGGACCAAGGATCTGGACACTGTCGAATTGCTGGAGCATTTGCCTGCTCTACAACAGCTTCTTTTTCGTGTACTTGGTTGCCAGCCACAAGGGGCAGCAGTTCATAATTTTGTGGTCCAGTTAGCACTTTCAATGGTTGCTTCAGAAAGCGTCAAGATTTATCAAGCTATTAGTGATGGAACGGTCAATTTGGTTGACAAGTTCTTTGAGATGCAACGTCATGATGCTCTGAAGGCTCTGGATATATATCGGAGGGCAGGACAGCAGGCAGAGAGACTGTCAGAGTTCTATGAAGTATGTAAGAGTCTTGATATTGGGCGAGGAGAAAAGTTTATTAAGATTGAGCAGCCCCCTGCATCATTTCTACAAGCCATGGAAGAGTACGTCAGAGAAGCTCCCCGTGTCTCAACAGTTCGCAAGGAGGTGATTGTTTCTGCCCCCAAGGAAATCTTAGCCATAGAGTACAAAAAGACCCAAGAGGATGAGGCACGTCCACCTTCACCACCTCCACCTGAACCAGTAAAACCTGAACCAGTAAAAGTGGTAGCACCTGTAGTTGAACCACCTGATTTGCTGGGTTTGAATGATCCTGTTCCAGACACTGCAGCATTAGATGAGAAGAATGCCATGGCTTTAGCTATTGTTCCAGTTTCTGATCAACCTACGGCTCAACCTCAAGCAAATGGAACAGGTTGGGAATTGGCACTTGTTACAGCCCCAAGCTCAAATGAGAGTGCTACAGCTGCTAGCAAACTGGCGGGAGGGTTGGACTTGCTTACACTAGACAGCCTATATGATGATGCAATTAGAAGAAACAATCAAAATGTAAGCTACAACCCATGGGAGCAGGGTCCCATGAATGGTGGCATGATGCAACAACCAATTCATGATCCTTTTTATGCATCCAACACAGTGGCCGCACCACCCTCTGTACAGATGGCCGCAATGGCCAACCAGCAGCAACAGGCCTTCATGTATCAACAACAACAGATGATGATGATGGGCCAACAACAACCGCAATCTATGAATCCTTTTGGGAATCCTCATGGAGCACCTGCCCACCCTTATGGAGCAGGTACGCCTGTCCAAGCATACAACCCATACACGGGCCTTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

544

Amino Acids

60.27

Weight (kDa)

6.05

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 35 - 301 9.8e-86 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1576
Acc36I ACCTGC 2 cut(s) 1576, 1577
AciI CCGC 5 cut(s) 269, 1271, 1431, 1455, 1529
AclWI GGATC 8 cut(s) 42, 55, 427, 440, 513, 531, 1094, 1397
AcoI YGGCCR 4 cut(s) 150, 1428, 1452, 1461
AcuI CTGAAG 2 cut(s) 639, 764
AfaI GTAC 7 cut(s) 19, 439, 585, 896, 977, 1446, 1591
AfiI CCNNNNNNNGG 3 cut(s) 1373, 1618, 1619
AflII CTTAAG 1 cut(s) 31
AgsI TTSAA 7 cut(s) 280, 391, 426, 487, 645, 1073, 1096
AjiI CACGTC 1 cut(s) 1004
AjnI CCWGG 1 cut(s) 428
AluBI AGCT 9 cut(s) 30, 173, 571, 680, 908, 1151, 1236, 1256, 1344
AluI AGCT 9 cut(s) 30, 173, 571, 680, 908, 1151, 1236, 1256, 1344
Alw21I GWGCWC 1 cut(s) 1567
Alw26I GTCTC 3 cut(s) 387, 781, 922
AlwI GGATC 8 cut(s) 42, 55, 427, 440, 513, 531, 1094, 1397
AlwNI CAGNNNCTG 2 cut(s) 1115, 1166
Ama87I CYCGRG 1 cut(s) 204
AoxI GGCC 7 cut(s) 150, 1428, 1452, 1461, 1479, 1516, 1621
ApeKI GCWGC 5 cut(s) 608, 859, 1118, 1256, 1471
ArsI GACNNNNNNTTYG 2 cut(s) 701, 733
AspS9I GGNCC 6 cut(s) 319, 516, 628, 1364, 1516, 1621
AsuHPI GGTGA 2 cut(s) 949, 1005
AsuNHI GCTAGC 1 cut(s) 1259
AvaI CYCGRG 1 cut(s) 204
AvaII GGWCC 4 cut(s) 319, 516, 628, 1364
BaeI ACNNNNGTAYC 2 cut(s) 1581, 1614
BalI TGGCCA 1 cut(s) 1463
BamHI GGATCC 2 cut(s) 47, 432
Bbv12I GWGCWC 1 cut(s) 1567
BbvI GCAGC 5 cut(s) 620, 871, 1130, 1243, 1483
BccI CCATC 4 cut(s) 496, 683, 1444, 1507
BceAI ACGGC 1 cut(s) 1194
BciT130I CCWGG 1 cut(s) 430
BclI TGATCA 1 cut(s) 1168
BcoDI GTCTC 3 cut(s) 387, 781, 922
BfaI CTAG 3 cut(s) 240, 1260, 1295
BfmI CTRYAG 3 cut(s) 1065, 1116, 1251
BfrI CTTAAG 1 cut(s) 31
BfuAI ACCTGC 2 cut(s) 1576, 1577
BglI GCCNNNNNGGC 1 cut(s) 1460
BisI GCNGC 7 cut(s) 609, 860, 1119, 1257, 1431, 1455, 1472
BlsI GCNGC 7 cut(s) 610, 861, 1120, 1258, 1432, 1456, 1473
Bme1390I CCNGG 1 cut(s) 430
Bme18I GGWCC 4 cut(s) 319, 516, 628, 1364
BmeT110I CYCGRG 1 cut(s) 204
BmgBI CACGTC 1 cut(s) 1004
BmgT120I GGNCC 6 cut(s) 319, 516, 628, 1364, 1516, 1621
BmiI GGNNCC 5 cut(s) 49, 321, 434, 1365, 1366
BmrFI CCNGG 1 cut(s) 430
BmrI ACTGGG 1 cut(s) 274
BmsI GCATC 7 cut(s) 234, 714, 727, 878, 1303, 1374, 1424
BmtI GCTAGC 1 cut(s) 1263
BmuI ACTGGG 1 cut(s) 274
BpmI CTGGAG 1 cut(s) 566
BpuEI CTTGAG 2 cut(s) 38, 1173
BsaBI GATNNNNATC 1 cut(s) 759
BsaJI CCNNGG 9 cut(s) 153, 203, 229, 512, 519, 885, 954, 1140, 1352
Bsc4I CCNNNNNNNGG 3 cut(s) 1373, 1618, 1619
Bse1I ACTGG 7 cut(s) 111, 269, 631, 1031, 1046, 1160, 1272
Bse3DI GCAATG 2 cut(s) 111, 1464
Bse8I GATNNNNATC 1 cut(s) 759
BseBI CCWGG 1 cut(s) 430
BseDI CCNNGG 9 cut(s) 153, 203, 229, 512, 519, 885, 954, 1140, 1352
BseGI GGATG 3 cut(s) 225, 1000, 1415
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 3 cut(s) 1373, 1618, 1619
BseMI GCAATG 2 cut(s) 111, 1464
BseNI ACTGG 7 cut(s) 111, 269, 631, 1031, 1046, 1160, 1272
BseRI GAGGAG 2 cut(s) 473, 849
BseXI GCAGC 5 cut(s) 620, 871, 1130, 1243, 1483
BseYI CCCAGC 1 cut(s) 1087
BshFI GGCC 7 cut(s) 152, 1430, 1454, 1463, 1481, 1518, 1623
BsiHKAI GWGCWC 1 cut(s) 1567
BsiHKCI CYCGRG 1 cut(s) 204
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 1350
BslI CCNNNNNNNGG 3 cut(s) 1373, 1618, 1619
BsmAI GTCTC 3 cut(s) 387, 781, 922
BsmFI GGGAC 1 cut(s) 1350
BsmI GAATGC 1 cut(s) 1141
BsnI GGCC 7 cut(s) 152, 1430, 1454, 1463, 1481, 1518, 1623
BsoBI CYCGRG 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 1567
Bsp1407I TGTACA 1 cut(s) 1444
Bsp143I GATC 7 cut(s) 47, 432, 505, 523, 1099, 1168, 1402
Bsp19I CCATGG 3 cut(s) 885, 1140, 1352
BspACI CCGC 5 cut(s) 269, 1271, 1431, 1455, 1529
BspANI GGCC 7 cut(s) 152, 1430, 1454, 1463, 1481, 1518, 1623
BspHI TCATGA 4 cut(s) 331, 342, 733, 1399
BspLI GGNNCC 5 cut(s) 49, 321, 434, 1365, 1366
BspMAI CTGCAG 1 cut(s) 1120
BspMI ACCTGC 2 cut(s) 1576, 1577
BspOI GCTAGC 1 cut(s) 1263
BspPI GGATC 8 cut(s) 42, 55, 427, 440, 513, 531, 1094, 1397
BspQI GCTCTTC 1 cut(s) 20
BspTI CTTAAG 1 cut(s) 31
BsrDI GCAATG 2 cut(s) 111, 1464
BsrGI TGTACA 1 cut(s) 1444
BsrI ACTGG 7 cut(s) 111, 269, 631, 1031, 1046, 1160, 1272
BssECI CCNNGG 9 cut(s) 153, 203, 229, 512, 519, 885, 954, 1140, 1352
BssMI GATC 7 cut(s) 47, 432, 505, 523, 1099, 1168, 1402
BssT1I CCWWGG 7 cut(s) 153, 512, 519, 885, 954, 1140, 1352
Bst2UI CCWGG 1 cut(s) 430
Bst4CI ACNGT 7 cut(s) 70, 233, 535, 697, 792, 925, 1426
Bst6I CTCTTC 2 cut(s) 20, 885
BstAFI CTTAAG 1 cut(s) 31
BstAUI TGTACA 1 cut(s) 1444
BstC8I GCNNGC 4 cut(s) 558, 596, 780, 1261
BstDEI CTNAG 1 cut(s) 964
BstDSI CCRYGG 4 cut(s) 229, 885, 1140, 1352
BstF5I GGATG 3 cut(s) 225, 1000, 1415
BstKTI GATC 7 cut(s) 50, 435, 508, 526, 1102, 1171, 1405
BstMAI GTCTC 3 cut(s) 387, 781, 922
BstMBI GATC 7 cut(s) 47, 432, 505, 523, 1099, 1168, 1402
BstMWI GCNNNNNNNGC 5 cut(s) 191, 215, 746, 1256, 1460
BstNI CCWGG 1 cut(s) 430
BstSCI CCNGG 1 cut(s) 428
BstSFI CTRYAG 3 cut(s) 1065, 1116, 1251
BstV1I GCAGC 5 cut(s) 620, 871, 1130, 1243, 1483
BstX2I RGATCY 3 cut(s) 47, 432, 523
BstXI CCANNNNNNTGG 4 cut(s) 414, 526, 1426, 1580
BstYI RGATCY 3 cut(s) 47, 432, 523
BsuRI GGCC 7 cut(s) 152, 1430, 1454, 1463, 1481, 1518, 1623
BtgI CCRYGG 4 cut(s) 229, 885, 1140, 1352
BtrI CACGTC 1 cut(s) 1004
BtsCI GGATG 3 cut(s) 225, 1000, 1415
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 3 cut(s) 531, 1113, 1431
BveI ACCTGC 2 cut(s) 1576, 1577
Cac8I GCNNGC 4 cut(s) 558, 596, 780, 1261
CaiI CAGNNNCTG 2 cut(s) 1115, 1166
CciI TCATGA 4 cut(s) 331, 342, 733, 1399
Cfr13I GGNCC 6 cut(s) 319, 516, 628, 1364, 1516, 1621
CseI GACGC 2 cut(s) 134, 652
Csp6I GTAC 7 cut(s) 18, 438, 584, 895, 976, 1445, 1590
CspCI CAANNNNNGTGG 2 cut(s) 1065, 1100
CviQI GTAC 7 cut(s) 18, 438, 584, 895, 976, 1445, 1590
DdeI CTNAG 1 cut(s) 964
DpnI GATC 7 cut(s) 49, 434, 507, 525, 1101, 1170, 1404
DpnII GATC 7 cut(s) 47, 432, 505, 523, 1099, 1168, 1402
EaeI YGGCCR 4 cut(s) 150, 1428, 1452, 1461
Eam1104I CTCTTC 2 cut(s) 20, 885
EarI CTCTTC 2 cut(s) 20, 885
Eco130I CCWWGG 7 cut(s) 153, 512, 519, 885, 954, 1140, 1352
Eco147I AGGCCT 1 cut(s) 1481
Eco47I GGWCC 4 cut(s) 319, 516, 628, 1364
Eco57I CTGAAG 2 cut(s) 639, 764
Eco88I CYCGRG 1 cut(s) 204
EcoO109I RGGNCCY 1 cut(s) 1364
EcoRII CCWGG 1 cut(s) 428
EcoT14I CCWWGG 7 cut(s) 153, 512, 519, 885, 954, 1140, 1352
EcoT22I ATGCAT 2 cut(s) 415, 1417
ErhI CCWWGG 7 cut(s) 153, 512, 519, 885, 954, 1140, 1352
FalI AAGNNNNNCTT 2 cut(s) 947, 979
FaqI GGGAC 1 cut(s) 1350
FauI CCCGC 1 cut(s) 1264
FauNDI CATATG 1 cut(s) 369
FbaI TGATCA 1 cut(s) 1168
Fnu4HI GCNGC 7 cut(s) 609, 860, 1119, 1257, 1431, 1455, 1472
FokI GGATG 3 cut(s) 212, 1007, 1402
Fsp4HI GCNGC 7 cut(s) 609, 860, 1119, 1257, 1431, 1455, 1472
FspBI CTAG 3 cut(s) 240, 1260, 1295
GluI GCNGC 7 cut(s) 609, 860, 1119, 1257, 1431, 1455, 1472
GsaI CCCAGC 1 cut(s) 1091
GsuI CTGGAG 1 cut(s) 566
HaeIII GGCC 7 cut(s) 152, 1430, 1454, 1463, 1481, 1518, 1623
HapII CCGG 1 cut(s) 149
HgaI GACGC 2 cut(s) 134, 652
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HinfI GANTC 5 cut(s) 398, 403, 817, 1540, 1552
HpaII CCGG 1 cut(s) 149
HphI GGTGA 2 cut(s) 949, 1005
Hpy166II GTNNAC 4 cut(s) 319, 584, 709, 1007
Hpy188I TCNGA 8 cut(s) 170, 288, 658, 744, 765, 796, 902, 1168
Hpy8I GTNNAC 4 cut(s) 319, 584, 709, 1007
HpyAV CCTTC 3 cut(s) 739, 1020, 1492
HpyCH4III ACNGT 7 cut(s) 70, 233, 535, 697, 792, 925, 1426
HpyCH4IV ACGT 3 cut(s) 730, 897, 1003
HpyF10VI GCNNNNNNNGC 5 cut(s) 191, 215, 746, 1256, 1460
HpyF3I CTNAG 1 cut(s) 964
HpySE526I ACGT 3 cut(s) 730, 897, 1003
KflI GGGWCCC 1 cut(s) 1364
Ksp22I TGATCA 1 cut(s) 1168
Kzo9I GATC 7 cut(s) 47, 432, 505, 523, 1099, 1168, 1402
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 5 cut(s) 547, 913, 1357, 1562, 1583
Lsp1109I GCAGC 5 cut(s) 620, 871, 1130, 1243, 1483
LweI GCATC 7 cut(s) 234, 714, 727, 878, 1303, 1374, 1424
MaeI CTAG 3 cut(s) 240, 1260, 1295
MaeII ACGT 3 cut(s) 730, 897, 1003
MaeIII GTNAC 1 cut(s) 1222
MalI GATC 7 cut(s) 49, 434, 507, 525, 1101, 1170, 1404
MboI GATC 7 cut(s) 47, 432, 505, 523, 1099, 1168, 1402
MboII GAAGA 5 cut(s) 37, 347, 902, 1144, 1335
MflI RGATCY 3 cut(s) 47, 432, 523
MhlI GDGCHC 1 cut(s) 1567
MlsI TGGCCA 1 cut(s) 1463
MluCI AATT 7 cut(s) 346, 539, 619, 700, 1211, 1317, 1395
MluNI TGGCCA 1 cut(s) 1463
MlyI GAGTC 2 cut(s) 412, 826
MmeI TCCRAC 2 cut(s) 1260, 1443
Mox20I TGGCCA 1 cut(s) 1463
Mph1103I ATGCAT 2 cut(s) 415, 1417
MscI TGGCCA 1 cut(s) 1463
MseI TTAA 3 cut(s) 32, 375, 849
MslI CAYNNNNRTG 1 cut(s) 1578
Msp20I TGGCCA 1 cut(s) 1463
MspA1I CMGCKG 1 cut(s) 1256
MspCI CTTAAG 1 cut(s) 31
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 1 cut(s) 430
Mva1269I GAATGC 1 cut(s) 1141
MvaI CCWGG 1 cut(s) 430
MwoI GCNNNNNNNGC 5 cut(s) 191, 215, 746, 1256, 1460
NcoI CCATGG 3 cut(s) 885, 1140, 1352
NdeI CATATG 1 cut(s) 369
NdeII GATC 7 cut(s) 47, 432, 505, 523, 1099, 1168, 1402
NheI GCTAGC 1 cut(s) 1259
NlaIV GGNNCC 5 cut(s) 49, 321, 434, 1365, 1366
NsiI ATGCAT 2 cut(s) 415, 1417
PagI TCATGA 4 cut(s) 331, 342, 733, 1399
PaqCI CACCTGC 1 cut(s) 1576
PceI AGGCCT 1 cut(s) 1481
PciSI GCTCTTC 1 cut(s) 20
PctI GAATGC 1 cut(s) 1141
PfeI GAWTC 3 cut(s) 398, 1540, 1552
PflFI GACNNNGTC 1 cut(s) 533
PkrI GCNGC 7 cut(s) 610, 861, 1120, 1258, 1432, 1456, 1473
PleI GAGTC 2 cut(s) 411, 825
PpsI GAGTC 2 cut(s) 411, 825
PpuMI RGGWCCY 1 cut(s) 1364
Psp5II RGGWCCY 1 cut(s) 1364
Psp6I CCWGG 1 cut(s) 428
PspFI CCCAGC 1 cut(s) 1087
PspGI CCWGG 1 cut(s) 428
PspN4I GGNNCC 5 cut(s) 49, 321, 434, 1365, 1366
PspPI GGNCC 6 cut(s) 319, 516, 628, 1364, 1516, 1621
PspPPI RGGWCCY 1 cut(s) 1364
PstI CTGCAG 1 cut(s) 1120
PstNI CAGNNNCTG 2 cut(s) 1115, 1166
PsuI RGATCY 3 cut(s) 47, 432, 523
PsyI GACNNNGTC 1 cut(s) 533
PvuII CAGCTG 1 cut(s) 1256
RsaI GTAC 7 cut(s) 19, 439, 585, 896, 977, 1446, 1591
RsaNI GTAC 7 cut(s) 18, 438, 584, 895, 976, 1445, 1590
RseI CAYNNNNRTG 1 cut(s) 1578
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 3 cut(s) 32, 375, 849
SatI GCNGC 7 cut(s) 609, 860, 1119, 1257, 1431, 1455, 1472
Sau3AI GATC 7 cut(s) 47, 432, 505, 523, 1099, 1168, 1402
Sau96I GGNCC 6 cut(s) 319, 516, 628, 1364, 1516, 1621
SchI GAGTC 2 cut(s) 412, 826
ScrFI CCNGG 1 cut(s) 430
SduI GDGCHC 1 cut(s) 1567
SfaNI GCATC 7 cut(s) 234, 714, 727, 878, 1303, 1374, 1424
SfcI CTRYAG 3 cut(s) 1065, 1116, 1251
SfiI GGCCNNNNNGGCC 1 cut(s) 1460
SinI GGWCC 4 cut(s) 319, 516, 628, 1364
SmiMI CAYNNNNRTG 1 cut(s) 1578
SmlI CTYRAG 3 cut(s) 31, 53, 1188
SmoI CTYRAG 3 cut(s) 31, 53, 1188
Sse9I AATT 7 cut(s) 346, 539, 619, 700, 1211, 1317, 1395
SseBI AGGCCT 1 cut(s) 1481
SsiI CCGC 5 cut(s) 269, 1271, 1431, 1455, 1529
SspMI CTAG 3 cut(s) 240, 1260, 1295
StuI AGGCCT 1 cut(s) 1481
StyD4I CCNGG 1 cut(s) 428
StyI CCWWGG 7 cut(s) 153, 512, 519, 885, 954, 1140, 1352
TaaI ACNGT 7 cut(s) 70, 233, 535, 697, 792, 925, 1426
TaiI ACGT 3 cut(s) 733, 900, 1006
TaqI TCGA 2 cut(s) 401, 537
TasI AATT 7 cut(s) 346, 539, 619, 700, 1211, 1317, 1395
TatI WGTACW 3 cut(s) 583, 975, 1444
TauI GCSGC 2 cut(s) 1433, 1457
TfiI GAWTC 3 cut(s) 398, 1540, 1552
Tru1I TTAA 3 cut(s) 32, 375, 849
Tru9I TTAA 3 cut(s) 32, 375, 849
TscAI CASTG 3 cut(s) 538, 1120, 1431
TseI GCWGC 5 cut(s) 608, 859, 1118, 1256, 1471
TspGWI ACGGA 1 cut(s) 425
TspRI CASTG 3 cut(s) 538, 1120, 1431
Tth111I GACNNNGTC 1 cut(s) 533
Vha464I CTTAAG 1 cut(s) 31
VpaK11BI GGWCC 4 cut(s) 319, 516, 628, 1364
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XspI CTAG 3 cut(s) 240, 1260, 1295
Zsp2I ATGCAT 2 cut(s) 415, 1417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.