Rroxscaffold_2G00153460

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004817
Physical Location & Seq
Reverse (-)
90259610 .. 90264790
5181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00153460.1

Sequence Viewer

Length: 1575 bp
ATGGCAGGAGGGGGTGGCACGCAGAAGAGCATAAGAAAAGTACTTGGATCCCTCAAGGACACCACCACAGTTTCATTGGCGAAAGTCAATAGCGATTACAAGGAACTGGACATTGCGATAGTCAAGGCAACGAATCATGTTGAGCGTCCTGCCAAGGAAAAATACATCAGAGCTATATTCTCTGCTGTTTCTGCAACAAGACCTCGGGCTGATGTTGCATATTGCATCCATGGTCTTGCTAGGAGGTTAGCGAAGACACATAACTGGGCGGTTGCATTGAAAACTCTAATTGTTATTCATCGTGCTTTGAGGGAAGTGGACCCCACCTTTCATGAAGAACTCATGAATTATGGTAGAAGTAGAAACCATATGCTTAACTTATCTCATTTCAAAGACGATTCCAGTCCAAATGCATGGGATTATTCAACCTGGATCCGTAGCTATGCCTTGTTTTTGGAGGAGAGATTGGAGTGTTTTCGTGTGTTGAAATATGATGTTGAGATGGATCGCCCGAGGACCAAGGATTTGGACACTGCTGAATTGCTTGAGCATTTGCCTGCTCTGCAACAGCTTCTTTTTCGTGTTCTTGGTTGCCAGGTTGCTTCAGAGAGCATCAAGATTTATCAAGCTATTAGTGACGGAACAGTCAATTTGATTGACAAGTTCTTTGAGATGCAACGCCATGATGCTATGAAGGCTCTGGATATATATCGGAGGGCAGGACAGCAGGCAGAGAGACTGTCAGAGTTCTATGAAGTATGTAAGAGTCTTGATATTGGGCGAGGAGAAAAGTTTATTAAGATTGAGCAGCCCCCTGCATCATTTTTACAAGCCATGGAAGAGTACGTCAGAGAAGCTCCCCGTGTTTCAATAGTTCGCAAGGAGGTGATTGACAATAAAATTTCTGCCCCCAAGGAAATCTTGGCCATAGAGGACAAAAAGACCCAAGAGGATGAGGCACGTCCACCTTCCCCACCTCCACCTGAACCAGTAAAAGTGGTAGCACCTGTAGTTGAACCACCTGATTTGCTGGGTTTGAATGATCCTGTTCCAGATACTGCAGCATTAGATGAGAAGAATGCCATGGCTTTAGCTATTGTTCCAGTTTCTGATCAACCTGCAGCTCAACCTCAAGCAAATGGAACAGGTTGGGAATTGGCACTTGTTACAGCCCCAAGTTCAAATGAGAGTGCCACAGCTGCTAGCAAACTGGCGGGAGGGTTGGACTTGCTTACACTAGACAGCCTATACGATGATGCAATTAGAAGAAACAATCAAAATGTAAGCTACAACCCATGGGAGCAGGCTCCCATGAATGGTGGCATGATGCAACAACCAATTCATGATCCCTTTTATGCATCCAACACAGTGGCCGCACCACCCTCTGTACAGATGGCCGCAATGGCCAACCAGCAGCAACAGGCCTTCATGTATCAACAACAGCAGATGATGATGATGGGCCAACAACAACCGCAATCTTTGAATCCTTTTGGGAATCCTCATGGAGCACCTGCCCACCCCTATGGAGCAGGTATGCCTGTCCAAGCATACAATCCATACACGGGCCTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

524

Amino Acids

58.3

Weight (kDa)

5.9

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 35 - 284 7e-74 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1519
AasI GACNNNNNNGTC 1 cut(s) 644
Acc36I ACCTGC 3 cut(s) 1126, 1519, 1520
AciI CCGC 5 cut(s) 269, 1214, 1374, 1398, 1472
AclWI GGATC 7 cut(s) 42, 55, 427, 440, 513, 1037, 1340
AcoI YGGCCR 4 cut(s) 924, 1371, 1395, 1404
AcsI RAATTY 1 cut(s) 900
AcuI CTGAAG 1 cut(s) 588
AfaI GTAC 3 cut(s) 42, 845, 1389
AfiI CCNNNNNNNGG 2 cut(s) 1316, 1562
AgsI TTSAA 9 cut(s) 280, 391, 426, 487, 870, 1016, 1039, 1182, 1483
AjiI CACGTC 1 cut(s) 962
AjnI CCWGG 2 cut(s) 428, 594
AluBI AGCT 9 cut(s) 173, 441, 571, 629, 857, 1094, 1124, 1199, 1287
AluI AGCT 9 cut(s) 173, 441, 571, 629, 857, 1094, 1124, 1199, 1287
Alw21I GWGCWC 1 cut(s) 1510
Alw26I GTCTC 1 cut(s) 730
AlwI GGATC 7 cut(s) 42, 55, 427, 440, 513, 1037, 1340
AlwNI CAGNNNCTG 2 cut(s) 1058, 1109
Ama87I CYCGRG 2 cut(s) 204, 511
AoxI GGCC 7 cut(s) 924, 1371, 1395, 1404, 1422, 1459, 1564
ApeKI GCWGC 5 cut(s) 808, 1061, 1121, 1199, 1414
ApoI RAATTY 1 cut(s) 900
ArsI GACNNNNNNTTYG 4 cut(s) 508, 540, 650, 682
AspS9I GGNCC 4 cut(s) 319, 516, 1459, 1564
AsuHPI GGTGA 1 cut(s) 898
AsuNHI GCTAGC 1 cut(s) 1202
AvaI CYCGRG 2 cut(s) 204, 511
AvaII GGWCC 2 cut(s) 319, 516
BalI TGGCCA 2 cut(s) 926, 1406
BamHI GGATCC 2 cut(s) 47, 432
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 1510
BbvI GCAGC 5 cut(s) 820, 1073, 1133, 1186, 1426
BccI CCATC 3 cut(s) 496, 1387, 1450
BciT130I CCWGG 2 cut(s) 430, 596
BclI TGATCA 1 cut(s) 1111
BcoDI GTCTC 1 cut(s) 730
BfaI CTAG 3 cut(s) 240, 1203, 1238
BfmI CTRYAG 3 cut(s) 1008, 1059, 1119
BfuAI ACCTGC 3 cut(s) 1126, 1519, 1520
BglI GCCNNNNNGGC 1 cut(s) 1403
BisI GCNGC 7 cut(s) 809, 1062, 1122, 1200, 1374, 1398, 1415
BlsI GCNGC 7 cut(s) 810, 1063, 1123, 1201, 1375, 1399, 1416
BmcAI AGTACT 1 cut(s) 42
Bme1390I CCNGG 2 cut(s) 430, 596
Bme18I GGWCC 2 cut(s) 319, 516
BmeT110I CYCGRG 2 cut(s) 204, 511
BmgBI CACGTC 1 cut(s) 962
BmgT120I GGNCC 4 cut(s) 319, 516, 1459, 1564
BmiI GGNNCC 4 cut(s) 49, 321, 434, 1308
BmrFI CCNGG 2 cut(s) 430, 596
BmrI ACTGGG 1 cut(s) 274
BmsI GCATC 8 cut(s) 234, 621, 663, 676, 827, 1246, 1317, 1367
BmtI GCTAGC 1 cut(s) 1206
BmuI ACTGGG 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 260
BpuEI CTTGAG 3 cut(s) 38, 566, 1116
BsaBI GATNNNNATC 1 cut(s) 708
BsaJI CCNNGG 9 cut(s) 153, 203, 229, 512, 519, 834, 912, 1083, 1295
Bsc4I CCNNNNNNNGG 2 cut(s) 1316, 1562
Bse1I ACTGG 6 cut(s) 111, 269, 402, 989, 1103, 1215
Bse3DI GCAATG 2 cut(s) 111, 1407
Bse8I GATNNNNATC 1 cut(s) 708
BseBI CCWGG 2 cut(s) 430, 596
BseDI CCNNGG 9 cut(s) 153, 203, 229, 512, 519, 834, 912, 1083, 1295
BseGI GGATG 3 cut(s) 225, 958, 1358
BseJI GATNNNNATC 1 cut(s) 708
BseLI CCNNNNNNNGG 2 cut(s) 1316, 1562
BseMI GCAATG 2 cut(s) 111, 1407
BseNI ACTGG 6 cut(s) 111, 269, 402, 989, 1103, 1215
BseRI GAGGAG 2 cut(s) 473, 798
BseXI GCAGC 5 cut(s) 820, 1073, 1133, 1186, 1426
BseYI CCCAGC 1 cut(s) 1030
BshFI GGCC 7 cut(s) 926, 1373, 1397, 1406, 1424, 1461, 1566
BsiHKAI GWGCWC 1 cut(s) 1510
BsiHKCI CYCGRG 2 cut(s) 204, 511
BslI CCNNNNNNNGG 2 cut(s) 1316, 1562
BsmAI GTCTC 1 cut(s) 730
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 7 cut(s) 926, 1373, 1397, 1406, 1424, 1461, 1566
BsoBI CYCGRG 2 cut(s) 204, 511
Bsp1286I GDGCHC 1 cut(s) 1510
Bsp1407I TGTACA 1 cut(s) 1387
Bsp143I GATC 6 cut(s) 47, 432, 505, 1042, 1111, 1345
Bsp19I CCATGG 4 cut(s) 229, 834, 1083, 1295
BspACI CCGC 5 cut(s) 269, 1214, 1374, 1398, 1472
BspANI GGCC 7 cut(s) 926, 1373, 1397, 1406, 1424, 1461, 1566
BspHI TCATGA 3 cut(s) 331, 342, 1342
BspLI GGNNCC 4 cut(s) 49, 321, 434, 1308
BspMAI CTGCAG 2 cut(s) 1063, 1123
BspMI ACCTGC 3 cut(s) 1126, 1519, 1520
BspOI GCTAGC 1 cut(s) 1206
BspPI GGATC 7 cut(s) 42, 55, 427, 440, 513, 1037, 1340
BspQI GCTCTTC 1 cut(s) 20
BsrDI GCAATG 2 cut(s) 111, 1407
BsrGI TGTACA 1 cut(s) 1387
BsrI ACTGG 6 cut(s) 111, 269, 402, 989, 1103, 1215
BssECI CCNNGG 9 cut(s) 153, 203, 229, 512, 519, 834, 912, 1083, 1295
BssMI GATC 6 cut(s) 47, 432, 505, 1042, 1111, 1345
BssT1I CCWWGG 7 cut(s) 153, 229, 519, 834, 912, 1083, 1295
Bst2UI CCWGG 2 cut(s) 430, 596
Bst4CI ACNGT 4 cut(s) 70, 646, 741, 1369
Bst6I CTCTTC 2 cut(s) 20, 834
BstAUI TGTACA 1 cut(s) 1387
BstC8I GCNNGC 5 cut(s) 20, 558, 729, 1204, 1305
BstDSI CCRYGG 4 cut(s) 229, 834, 1083, 1295
BstF5I GGATG 3 cut(s) 225, 958, 1358
BstKTI GATC 6 cut(s) 50, 435, 508, 1045, 1114, 1348
BstMAI GTCTC 1 cut(s) 730
BstMBI GATC 6 cut(s) 47, 432, 505, 1042, 1111, 1345
BstMWI GCNNNNNNNGC 6 cut(s) 191, 215, 562, 695, 1199, 1403
BstNI CCWGG 2 cut(s) 430, 596
BstSCI CCNGG 2 cut(s) 428, 594
BstSFI CTRYAG 3 cut(s) 1008, 1059, 1119
BstV1I GCAGC 5 cut(s) 820, 1073, 1133, 1186, 1426
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 2 cut(s) 47, 432
BstXI CCANNNNNNTGG 4 cut(s) 414, 526, 1369, 1523
BstYI RGATCY 2 cut(s) 47, 432
BsuRI GGCC 7 cut(s) 926, 1373, 1397, 1406, 1424, 1461, 1566
BtgI CCRYGG 4 cut(s) 229, 834, 1083, 1295
BtrI CACGTC 1 cut(s) 962
BtsCI GGATG 3 cut(s) 225, 958, 1358
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 2 cut(s) 531, 1374
BveI ACCTGC 3 cut(s) 1126, 1519, 1520
Cac8I GCNNGC 5 cut(s) 20, 558, 729, 1204, 1305
CaiI CAGNNNCTG 2 cut(s) 1058, 1109
CciI TCATGA 3 cut(s) 331, 342, 1342
Cfr13I GGNCC 4 cut(s) 319, 516, 1459, 1564
CseI GACGC 1 cut(s) 134
Csp6I GTAC 3 cut(s) 41, 844, 1388
CspCI CAANNNNNGTGG 2 cut(s) 1008, 1043
CviQI GTAC 3 cut(s) 41, 844, 1388
DpnI GATC 6 cut(s) 49, 434, 507, 1044, 1113, 1347
DpnII GATC 6 cut(s) 47, 432, 505, 1042, 1111, 1345
DrdI GACNNNNNNGTC 1 cut(s) 644
DseDI GACNNNNNNGTC 1 cut(s) 644
EaeI YGGCCR 4 cut(s) 924, 1371, 1395, 1404
Eam1104I CTCTTC 2 cut(s) 20, 834
EarI CTCTTC 2 cut(s) 20, 834
Eco130I CCWWGG 7 cut(s) 153, 229, 519, 834, 912, 1083, 1295
Eco147I AGGCCT 1 cut(s) 1424
Eco47I GGWCC 2 cut(s) 319, 516
Eco57I CTGAAG 1 cut(s) 588
Eco88I CYCGRG 2 cut(s) 204, 511
EcoRII CCWGG 2 cut(s) 428, 594
EcoT14I CCWWGG 7 cut(s) 153, 229, 519, 834, 912, 1083, 1295
EcoT22I ATGCAT 2 cut(s) 415, 1360
ErhI CCWWGG 7 cut(s) 153, 229, 519, 834, 912, 1083, 1295
FalI AAGNNNNNCTT 2 cut(s) 905, 937
FauI CCCGC 1 cut(s) 1207
FauNDI CATATG 1 cut(s) 369
FbaI TGATCA 1 cut(s) 1111
Fnu4HI GCNGC 7 cut(s) 809, 1062, 1122, 1200, 1374, 1398, 1415
FokI GGATG 3 cut(s) 212, 965, 1345
Fsp4HI GCNGC 7 cut(s) 809, 1062, 1122, 1200, 1374, 1398, 1415
FspBI CTAG 3 cut(s) 240, 1203, 1238
GluI GCNGC 7 cut(s) 809, 1062, 1122, 1200, 1374, 1398, 1415
GsaI CCCAGC 1 cut(s) 1034
HaeIII GGCC 7 cut(s) 926, 1373, 1397, 1406, 1424, 1461, 1566
HgaI GACGC 1 cut(s) 134
HinfI GANTC 5 cut(s) 133, 398, 766, 1483, 1495
HphI GGTGA 1 cut(s) 898
Hpy166II GTNNAC 2 cut(s) 319, 965
Hpy188I TCNGA 6 cut(s) 170, 607, 714, 745, 851, 1111
Hpy188III TCNNGA 7 cut(s) 332, 343, 616, 701, 770, 1052, 1343
Hpy8I GTNNAC 2 cut(s) 319, 965
HpyAV CCTTC 3 cut(s) 688, 978, 1435
HpyCH4III ACNGT 4 cut(s) 70, 646, 741, 1369
HpyCH4IV ACGT 2 cut(s) 846, 961
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 215, 562, 695, 1199, 1403
HpySE526I ACGT 2 cut(s) 846, 961
Ksp22I TGATCA 1 cut(s) 1111
Kzo9I GATC 6 cut(s) 47, 432, 505, 1042, 1111, 1345
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 5 cut(s) 862, 1300, 1312, 1505, 1526
Lsp1109I GCAGC 5 cut(s) 820, 1073, 1133, 1186, 1426
LweI GCATC 8 cut(s) 234, 621, 663, 676, 827, 1246, 1317, 1367
MaeI CTAG 3 cut(s) 240, 1203, 1238
MaeII ACGT 2 cut(s) 846, 961
MaeIII GTNAC 2 cut(s) 635, 1165
MalI GATC 6 cut(s) 49, 434, 507, 1044, 1113, 1347
MboI GATC 6 cut(s) 47, 432, 505, 1042, 1111, 1345
MboII GAAGA 6 cut(s) 37, 265, 347, 851, 1087, 1278
MflI RGATCY 2 cut(s) 47, 432
MhlI GDGCHC 1 cut(s) 1510
MlsI TGGCCA 2 cut(s) 926, 1406
MluCI AATT 8 cut(s) 288, 346, 539, 649, 900, 1154, 1260, 1338
MluNI TGGCCA 2 cut(s) 926, 1406
MlyI GAGTC 1 cut(s) 775
MmeI TCCRAC 2 cut(s) 1203, 1386
Mox20I TGGCCA 2 cut(s) 926, 1406
Mph1103I ATGCAT 2 cut(s) 415, 1360
MscI TGGCCA 2 cut(s) 926, 1406
MseI TTAA 2 cut(s) 375, 798
MslI CAYNNNNRTG 1 cut(s) 1521
Msp20I TGGCCA 2 cut(s) 926, 1406
MspA1I CMGCKG 1 cut(s) 1199
MspR9I CCNGG 2 cut(s) 430, 596
Mva1269I GAATGC 1 cut(s) 1084
MvaI CCWGG 2 cut(s) 430, 596
MwoI GCNNNNNNNGC 6 cut(s) 191, 215, 562, 695, 1199, 1403
NcoI CCATGG 4 cut(s) 229, 834, 1083, 1295
NdeI CATATG 1 cut(s) 369
NdeII GATC 6 cut(s) 47, 432, 505, 1042, 1111, 1345
NheI GCTAGC 1 cut(s) 1202
NlaIV GGNNCC 4 cut(s) 49, 321, 434, 1308
NmuCI GTSAC 1 cut(s) 635
NsiI ATGCAT 2 cut(s) 415, 1360
PagI TCATGA 3 cut(s) 331, 342, 1342
PaqCI CACCTGC 1 cut(s) 1519
PceI AGGCCT 1 cut(s) 1424
PciSI GCTCTTC 1 cut(s) 20
PctI GAATGC 1 cut(s) 1084
PfeI GAWTC 4 cut(s) 133, 398, 1483, 1495
PkrI GCNGC 7 cut(s) 810, 1063, 1123, 1201, 1375, 1399, 1416
PleI GAGTC 1 cut(s) 774
PpsI GAGTC 1 cut(s) 774
Psp6I CCWGG 2 cut(s) 428, 594
PspFI CCCAGC 1 cut(s) 1030
PspGI CCWGG 2 cut(s) 428, 594
PspN4I GGNNCC 4 cut(s) 49, 321, 434, 1308
PspPI GGNCC 4 cut(s) 319, 516, 1459, 1564
PstI CTGCAG 2 cut(s) 1063, 1123
PstNI CAGNNNCTG 2 cut(s) 1058, 1109
PsuI RGATCY 2 cut(s) 47, 432
PvuII CAGCTG 1 cut(s) 1199
RsaI GTAC 3 cut(s) 42, 845, 1389
RsaNI GTAC 3 cut(s) 41, 844, 1388
RseI CAYNNNNRTG 1 cut(s) 1521
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 2 cut(s) 375, 798
SatI GCNGC 7 cut(s) 809, 1062, 1122, 1200, 1374, 1398, 1415
Sau3AI GATC 6 cut(s) 47, 432, 505, 1042, 1111, 1345
Sau96I GGNCC 4 cut(s) 319, 516, 1459, 1564
ScaI AGTACT 1 cut(s) 42
SchI GAGTC 1 cut(s) 775
ScrFI CCNGG 2 cut(s) 430, 596
SduI GDGCHC 1 cut(s) 1510
SfaNI GCATC 8 cut(s) 234, 621, 663, 676, 827, 1246, 1317, 1367
SfcI CTRYAG 3 cut(s) 1008, 1059, 1119
SfiI GGCCNNNNNGGCC 1 cut(s) 1403
SinI GGWCC 2 cut(s) 319, 516
SmiMI CAYNNNNRTG 1 cut(s) 1521
SmlI CTYRAG 3 cut(s) 53, 545, 1131
SmoI CTYRAG 3 cut(s) 53, 545, 1131
Sse9I AATT 8 cut(s) 288, 346, 539, 649, 900, 1154, 1260, 1338
SseBI AGGCCT 1 cut(s) 1424
SsiI CCGC 5 cut(s) 269, 1214, 1374, 1398, 1472
SspMI CTAG 3 cut(s) 240, 1203, 1238
StuI AGGCCT 1 cut(s) 1424
StyD4I CCNGG 2 cut(s) 428, 594
StyI CCWWGG 7 cut(s) 153, 229, 519, 834, 912, 1083, 1295
TaaI ACNGT 4 cut(s) 70, 646, 741, 1369
TaiI ACGT 2 cut(s) 849, 964
TasI AATT 8 cut(s) 288, 346, 539, 649, 900, 1154, 1260, 1338
TatI WGTACW 2 cut(s) 40, 1387
TauI GCSGC 2 cut(s) 1376, 1400
TfiI GAWTC 4 cut(s) 133, 398, 1483, 1495
Tru1I TTAA 2 cut(s) 375, 798
Tru9I TTAA 2 cut(s) 375, 798
TscAI CASTG 2 cut(s) 538, 1374
TseFI GTSAC 1 cut(s) 635
TseI GCWGC 5 cut(s) 808, 1061, 1121, 1199, 1414
Tsp45I GTSAC 1 cut(s) 635
TspGWI ACGGA 2 cut(s) 425, 654
TspRI CASTG 2 cut(s) 538, 1374
VpaK11BI GGWCC 2 cut(s) 319, 516
XapI RAATTY 1 cut(s) 900
XcmI CCANNNNNNNNNTGG 2 cut(s) 73, 919
XspI CTAG 3 cut(s) 240, 1203, 1238
ZrmI AGTACT 1 cut(s) 42
Zsp2I ATGCAT 2 cut(s) 415, 1360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.