MD02G1022900.v1.1

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004817
Physical Location & Seq
Forward (+)
1599577 .. 1604250
4674 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1022900.v1.1.491

Sequence Viewer

Length: 1644 bp
ATGTCAGGAGGTACACAGAAGAGCATCAGGAAAGCTCTTGGAGCCCTCAAGGACACCACCACAGTTTCATTGGCTAAAGTCAACAGCGATTACAAGGAATTGGACATTGCGATAGTCAAAGCTACGAACCATGTTGAGCGTCCCGCCAAGGAAAAGTACATTAGAGCTATTTTTGCTGCCGTTTCTGCTACAAGACCTCGTGCTGATGTTGCATACTGCATCCACGCTCTTGCAAGGAGGTTAGCAAAGACACATAACTGGGCGGTTGCATTGAAGACATTAGTTGTTATTCATCGTGCTTTGAGGGAAGTGGACCCCACGTTTTACGAAGAACTCATGAATTATGGTAGAAGTAGAAGCCATATGCTTAACTTGGCACATTTCAAAGATGATTCCAGTCCCAATGCATGGGATTTTTCCACCTGGATCCGCACTTATGCTTTGTTCTTGGAGGAGAGACTGGAATGCTTTCGTGTGTTGAAATATGACGTTGAGATGGATCGCCCAAGGACCAAAGATCTGGATACTGCCGAATTGCTTGAGCATTTGTCTGCCCTGCAACAACTTCTTTTTCGTGTACTTGGTTGCCAGCCGCAAGGGGCAGCAGTTCATAATTTTGTGGTTCAGTTAGCACTCTCAATGGTTGCTTCTGAAAGCATCAAGATTTATCAAGCTATAAGTGATGGAACAGTCAATTTGGTTGACAAGTTCTTTGAGATGCAACGCCATGATGCTATGAGGGCGCTGGACATATATCGGAGGGCAGGACAGCAGGCAGAGAGGCTGTCAGAGTTTTATGAAGTATGTAAAAGTCTTGATATTGGACGTGGAGAGAAGTTTACCAAGATTGAGCAGCCCCCTGCATCGTTTTTACAAGCCATGGAAGAGTACGTGAAAGAAGCTCCTCAGTTCTCAACAGCTCGCAAGGATCAGGTGGTTGCCCCCAAAGAAATCTTGGCTATAGAGTACAAAAAGGCCCCGGAGGAGGAGGCACGTCCACCTTCACCACCTCTACCTGAACCAGTGAAAGTTGAACCAGTGAAAGTGGAAGCACCTGTAGCTGAACCGCCTGATTTGCTGGGTTTGAATGATCCTGTTCCAAATACTAAAGAGTTGGATGACAAGAATGCACTGGCTTTAGCGATTGTTCCAGTTTCGGACCAACCAACATCCACAGCTCCAACTCTAGAAAATGGAGCTACAGGTTGGGAATTGGCACTTGTTACAGCACCGAGCTCAAATGAGAGCGCCGTGGCTTCTAGCAAACTGGCGGGAGGGTTAGACTTGCTTACACTAGACAGCCTATATGACGATGCAATCAGAAGAAACAATCAGAATGTGAGCTACAACCCATGGGAGCCTGCTCCGGTAAATGGTACCATGATGCAACAACAACTTCACGATCCCTTTTATGCATCCAACGGAATGGCTGCACCGCATTCTGTGCAGATGGCAGCAATGGCGAACCAGCAGCAGGCCTTCATGTTGCAGCAACAGCAGCAGCAGCAGCAGATGATGATGATGATGGGCCAACAACAGCAACAGTCTATGAATCCTTTCGGAACTCCTCACGGAGCCCCTACCCACCCCTACGGCCCCGGTGTCCCTGTCCAAGCATACAACCCATACGCAGGCCTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

548

Amino Acids

60.89

Weight (kDa)

5.87

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 33 - 299 7.3e-85 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1376
AccB1I GGYRCC 1 cut(s) 1376
AccB7I CCANNNNNTGG 1 cut(s) 408
AciI CCGC 7 cut(s) 144, 263, 430, 593, 1067, 1271, 1436
AclWI GGATC 6 cut(s) 421, 434, 507, 936, 1085, 1397
AfaI GTAC 6 cut(s) 13, 158, 579, 890, 968, 1378
AfiI CCNNNNNNNGG 5 cut(s) 408, 985, 1373, 1474, 1631
AgsI TTSAA 5 cut(s) 274, 385, 481, 1034, 1087
AjiI CACGTC 2 cut(s) 827, 995
AjnI CCWGG 1 cut(s) 422
Alw21I GWGCWC 1 cut(s) 1238
Alw26I GTCTC 1 cut(s) 451
AlwI GGATC 6 cut(s) 421, 434, 507, 936, 1085, 1397
AoxI GGCC 5 cut(s) 975, 1476, 1528, 1594, 1633
ArsI GACNNNNNNTTYG 2 cut(s) 695, 727
Asp700I GAANNNNTTC 2 cut(s) 468, 1556
Asp718I GGTACC 1 cut(s) 1376
AspLEI GCGC 2 cut(s) 745, 1250
AspS9I GGNCC 6 cut(s) 313, 510, 976, 1159, 1528, 1595
AsuC2I CCSGG 2 cut(s) 980, 1599
AsuHPI GGTGA 1 cut(s) 996
AvaII GGWCC 3 cut(s) 313, 510, 1159
BamHI GGATCC 1 cut(s) 426
BanI GGYRCC 1 cut(s) 1376
BanII GRGCYC 3 cut(s) 46, 1238, 1579
BauI CACGAG 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 1238
BccI CCATC 4 cut(s) 490, 677, 1444, 1519
BceAI ACGGC 3 cut(s) 164, 1235, 1609
BciT130I CCWGG 1 cut(s) 424
BciVI GTATCC 1 cut(s) 517
BcnI CCSGG 2 cut(s) 980, 1599
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 3 cut(s) 1187, 1260, 1295
BfmI CTRYAG 3 cut(s) 960, 1056, 1200
BfoI RGCGCY 2 cut(s) 746, 1251
BfuI GTATCC 1 cut(s) 517
BglII AGATCT 1 cut(s) 517
Bme1390I CCNGG 3 cut(s) 424, 980, 1599
Bme18I GGWCC 3 cut(s) 313, 510, 1159
BmgBI CACGTC 2 cut(s) 827, 995
BmgT120I GGNCC 6 cut(s) 313, 510, 976, 1159, 1528, 1595
BmiI GGNNCC 8 cut(s) 43, 315, 428, 978, 1359, 1378, 1576, 1597
BmrFI CCNGG 3 cut(s) 424, 980, 1599
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 9 cut(s) 33, 228, 666, 708, 721, 872, 1303, 1374, 1424
BmuI ACTGGG 1 cut(s) 268
BpiI GAAGAC 1 cut(s) 281
BpuEI CTTGAG 2 cut(s) 32, 560
BpuMI CCSGG 2 cut(s) 980, 1599
BsaAI YACGTR 1 cut(s) 892
BsaJI CCNNGG 7 cut(s) 147, 506, 879, 978, 1251, 1352, 1597
BsaWI WCCGGW 1 cut(s) 1366
Bsc4I CCNNNNNNNGG 5 cut(s) 408, 985, 1373, 1474, 1631
Bse1I ACTGG 8 cut(s) 263, 396, 465, 1022, 1037, 1137, 1151, 1272
Bse3DI GCAATG 2 cut(s) 105, 1464
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 7 cut(s) 147, 506, 879, 978, 1251, 1352, 1597
BseGI GGATG 4 cut(s) 219, 1123, 1169, 1415
BseLI CCNNNNNNNGG 5 cut(s) 408, 985, 1373, 1474, 1631
BseMI GCAATG 2 cut(s) 105, 1464
BseMII CTCAG 1 cut(s) 920
BseNI ACTGG 8 cut(s) 263, 396, 465, 1022, 1037, 1137, 1151, 1272
BseRI GAGGAG 5 cut(s) 467, 894, 998, 1001, 1557
BseYI CCCAGC 1 cut(s) 1078
BsgI GTGCAG 2 cut(s) 1416, 1466
BshFI GGCC 5 cut(s) 977, 1478, 1530, 1596, 1635
BshNI GGYRCC 1 cut(s) 1376
BsiHKAI GWGCWC 1 cut(s) 1238
BsiSI CCGG 3 cut(s) 980, 1367, 1599
BslFI GGGAC 3 cut(s) 126, 384, 1589
BslI CCNNNNNNNGG 5 cut(s) 408, 985, 1373, 1474, 1631
BsmAI GTCTC 1 cut(s) 451
BsmFI GGGAC 3 cut(s) 126, 384, 1589
BsmI GAATGC 3 cut(s) 470, 1132, 1438
BsnI GGCC 5 cut(s) 977, 1478, 1530, 1596, 1635
Bsp1286I GDGCHC 3 cut(s) 46, 1238, 1579
Bsp143I GATC 6 cut(s) 426, 499, 517, 928, 1090, 1402
Bsp19I CCATGG 2 cut(s) 879, 1352
BspACI CCGC 7 cut(s) 144, 263, 430, 593, 1067, 1271, 1436
BspANI GGCC 5 cut(s) 977, 1478, 1530, 1596, 1635
BspCNI CTCAG 1 cut(s) 919
BspHI TCATGA 1 cut(s) 336
BspLI GGNNCC 8 cut(s) 43, 315, 428, 978, 1359, 1378, 1576, 1597
BspPI GGATC 6 cut(s) 421, 434, 507, 936, 1085, 1397
BspQI GCTCTTC 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 1376
BsrDI GCAATG 2 cut(s) 105, 1464
BsrI ACTGG 8 cut(s) 263, 396, 465, 1022, 1037, 1137, 1151, 1272
BssECI CCNNGG 7 cut(s) 147, 506, 879, 978, 1251, 1352, 1597
BssMI GATC 6 cut(s) 426, 499, 517, 928, 1090, 1402
BssSI CACGAG 1 cut(s) 198
BssT1I CCWWGG 4 cut(s) 147, 506, 879, 1352
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 3 cut(s) 64, 691, 1545
Bst6I CTCTTC 2 cut(s) 14, 879
BstAPI GCANNNNNTGC 1 cut(s) 1444
BstBAI YACGTR 1 cut(s) 892
BstC8I GCNNGC 6 cut(s) 590, 774, 922, 1362, 1476, 1633
BstDEI CTNAG 1 cut(s) 906
BstDSI CCRYGG 3 cut(s) 879, 1251, 1352
BstF5I GGATG 4 cut(s) 219, 1123, 1169, 1415
BstH2I RGCGCY 2 cut(s) 746, 1251
BstHHI GCGC 2 cut(s) 745, 1250
BstKTI GATC 6 cut(s) 429, 502, 520, 931, 1093, 1405
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 6 cut(s) 426, 499, 517, 928, 1090, 1402
BstNI CCWGG 1 cut(s) 424
BstSCI CCNGG 3 cut(s) 422, 978, 1597
BstSFI CTRYAG 3 cut(s) 960, 1056, 1200
BstV2I GAAGAC 1 cut(s) 281
BstX2I RGATCY 2 cut(s) 426, 517
BstXI CCANNNNNNTGG 2 cut(s) 520, 1426
BstYI RGATCY 2 cut(s) 426, 517
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 5 cut(s) 977, 1478, 1530, 1596, 1635
BtgI CCRYGG 3 cut(s) 879, 1251, 1352
BtrI CACGTC 2 cut(s) 827, 995
BtsCI GGATG 4 cut(s) 219, 1123, 1169, 1415
BtsIMutI CAGTG 3 cut(s) 1029, 1044, 1130
Cac8I GCNNGC 6 cut(s) 590, 774, 922, 1362, 1476, 1633
CciI TCATGA 1 cut(s) 336
CfoI GCGC 2 cut(s) 745, 1250
Cfr13I GGNCC 6 cut(s) 313, 510, 976, 1159, 1528, 1595
CseI GACGC 1 cut(s) 128
Csp6I GTAC 6 cut(s) 12, 157, 578, 889, 967, 1377
CviAII CATG 8 cut(s) 131, 337, 408, 728, 880, 1353, 1381, 1483
CviQI GTAC 6 cut(s) 12, 157, 578, 889, 967, 1377
DdeI CTNAG 1 cut(s) 906
DpnI GATC 6 cut(s) 428, 501, 519, 930, 1092, 1404
DpnII GATC 6 cut(s) 426, 499, 517, 928, 1090, 1402
Eam1104I CTCTTC 2 cut(s) 14, 879
EarI CTCTTC 2 cut(s) 14, 879
Ecl136II GAGCTC 1 cut(s) 1236
Eco130I CCWWGG 4 cut(s) 147, 506, 879, 1352
Eco147I AGGCCT 2 cut(s) 1478, 1635
Eco24I GRGCYC 3 cut(s) 46, 1238, 1579
Eco47I GGWCC 3 cut(s) 313, 510, 1159
Eco53kI GAGCTC 1 cut(s) 1236
EcoICRI GAGCTC 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 976
EcoRII CCWGG 1 cut(s) 422
EcoT14I CCWWGG 4 cut(s) 147, 506, 879, 1352
EcoT22I ATGCAT 2 cut(s) 409, 1417
EcoT38I GRGCYC 3 cut(s) 46, 1238, 1579
ErhI CCWWGG 4 cut(s) 147, 506, 879, 1352
FaeI CATG 8 cut(s) 134, 340, 411, 731, 883, 1356, 1384, 1486
FaqI GGGAC 3 cut(s) 126, 384, 1589
FatI CATG 8 cut(s) 130, 336, 407, 727, 879, 1352, 1380, 1482
FauI CCCGC 2 cut(s) 151, 1264
FauNDI CATATG 1 cut(s) 363
FokI GGATG 4 cut(s) 206, 1130, 1156, 1402
FriOI GRGCYC 3 cut(s) 46, 1238, 1579
FspBI CTAG 3 cut(s) 1187, 1260, 1295
GlaI GCGC 2 cut(s) 744, 1249
GsaI CCCAGC 1 cut(s) 1082
HaeII RGCGCY 2 cut(s) 746, 1251
HaeIII GGCC 5 cut(s) 977, 1478, 1530, 1596, 1635
HapII CCGG 3 cut(s) 980, 1367, 1599
HgaI GACGC 1 cut(s) 128
HhaI GCGC 2 cut(s) 745, 1250
Hin1II CATG 8 cut(s) 134, 340, 411, 731, 883, 1356, 1384, 1486
Hin6I GCGC 2 cut(s) 743, 1248
HinP1I GCGC 2 cut(s) 743, 1248
HincII GTYRAC 2 cut(s) 82, 703
HindII GTYRAC 2 cut(s) 82, 703
HinfI GANTC 2 cut(s) 392, 1552
HpaII CCGG 3 cut(s) 980, 1367, 1599
HphI GGTGA 1 cut(s) 996
Hpy166II GTNNAC 7 cut(s) 14, 82, 313, 578, 703, 840, 998
Hpy188I TCNGA 7 cut(s) 652, 759, 790, 1159, 1322, 1335, 1562
Hpy188III TCNNGA 8 cut(s) 6, 28, 337, 521, 661, 815, 1187, 1400
Hpy8I GTNNAC 7 cut(s) 14, 82, 313, 578, 703, 840, 998
HpyAV CCTTC 2 cut(s) 1011, 1489
HpyCH4III ACNGT 3 cut(s) 64, 691, 1545
HpyCH4IV ACGT 5 cut(s) 320, 489, 826, 891, 994
HpyF3I CTNAG 1 cut(s) 906
HpySE526I ACGT 5 cut(s) 320, 489, 826, 891, 994
Hsp92II CATG 8 cut(s) 134, 340, 411, 731, 883, 1356, 1384, 1486
HspAI GCGC 2 cut(s) 743, 1248
KpnI GGTACC 1 cut(s) 1380
Kzo9I GATC 6 cut(s) 426, 499, 517, 928, 1090, 1402
LguI GCTCTTC 1 cut(s) 14
LmnI GCTCC 7 cut(s) 41, 907, 1183, 1196, 1357, 1369, 1574
LweI GCATC 9 cut(s) 33, 228, 666, 708, 721, 872, 1303, 1374, 1424
MaeI CTAG 3 cut(s) 1187, 1260, 1295
MaeII ACGT 5 cut(s) 320, 489, 826, 891, 994
MaeIII GTNAC 1 cut(s) 1222
MalI GATC 6 cut(s) 428, 501, 519, 930, 1092, 1404
MboI GATC 6 cut(s) 426, 499, 517, 928, 1090, 1402
MboII GAAGA 5 cut(s) 31, 286, 341, 896, 1335
MflI RGATCY 2 cut(s) 426, 517
MhlI GDGCHC 3 cut(s) 46, 1238, 1579
MluCI AATT 6 cut(s) 98, 340, 533, 613, 694, 1211
MmeI TCCRAC 3 cut(s) 1095, 1205, 1443
Mph1103I ATGCAT 2 cut(s) 409, 1417
MroXI GAANNNNTTC 2 cut(s) 468, 1556
MseI TTAA 1 cut(s) 369
MspI CCGG 3 cut(s) 980, 1367, 1599
MspR9I CCNGG 3 cut(s) 424, 980, 1599
Mva1269I GAATGC 3 cut(s) 470, 1132, 1438
MvaI CCWGG 1 cut(s) 424
NciI CCSGG 2 cut(s) 980, 1599
NcoI CCATGG 2 cut(s) 879, 1352
NdeI CATATG 1 cut(s) 363
NdeII GATC 6 cut(s) 426, 499, 517, 928, 1090, 1402
NlaIII CATG 8 cut(s) 134, 340, 411, 731, 883, 1356, 1384, 1486
NlaIV GGNNCC 8 cut(s) 43, 315, 428, 978, 1359, 1378, 1576, 1597
NsiI ATGCAT 2 cut(s) 409, 1417
PagI TCATGA 1 cut(s) 336
PceI AGGCCT 2 cut(s) 1478, 1635
PciSI GCTCTTC 1 cut(s) 14
PctI GAATGC 3 cut(s) 470, 1132, 1438
PdmI GAANNNNTTC 2 cut(s) 468, 1556
PfeI GAWTC 2 cut(s) 392, 1552
PflMI CCANNNNNTGG 1 cut(s) 408
Ppu21I YACGTR 1 cut(s) 892
Psp124BI GAGCTC 1 cut(s) 1238
Psp6I CCWGG 1 cut(s) 422
PspFI CCCAGC 1 cut(s) 1078
PspGI CCWGG 1 cut(s) 422
PspN4I GGNNCC 8 cut(s) 43, 315, 428, 978, 1359, 1378, 1576, 1597
PspPI GGNCC 6 cut(s) 313, 510, 976, 1159, 1528, 1595
PsuI RGATCY 2 cut(s) 426, 517
RsaI GTAC 6 cut(s) 13, 158, 579, 890, 968, 1378
RsaNI GTAC 6 cut(s) 12, 157, 578, 889, 967, 1377
SacI GAGCTC 1 cut(s) 1238
SapI GCTCTTC 1 cut(s) 14
SaqAI TTAA 1 cut(s) 369
Sau3AI GATC 6 cut(s) 426, 499, 517, 928, 1090, 1402
Sau96I GGNCC 6 cut(s) 313, 510, 976, 1159, 1528, 1595
ScrFI CCNGG 3 cut(s) 424, 980, 1599
SduI GDGCHC 3 cut(s) 46, 1238, 1579
SfaNI GCATC 9 cut(s) 33, 228, 666, 708, 721, 872, 1303, 1374, 1424
SfcI CTRYAG 3 cut(s) 960, 1056, 1200
SinI GGWCC 3 cut(s) 313, 510, 1159
SmlI CTYRAG 2 cut(s) 47, 539
SmoI CTYRAG 2 cut(s) 47, 539
Sse9I AATT 6 cut(s) 98, 340, 533, 613, 694, 1211
SseBI AGGCCT 2 cut(s) 1478, 1635
SsiI CCGC 7 cut(s) 144, 263, 430, 593, 1067, 1271, 1436
SspMI CTAG 3 cut(s) 1187, 1260, 1295
SstI GAGCTC 1 cut(s) 1238
StuI AGGCCT 2 cut(s) 1478, 1635
StyD4I CCNGG 3 cut(s) 422, 978, 1597
StyI CCWWGG 4 cut(s) 147, 506, 879, 1352
TaaI ACNGT 3 cut(s) 64, 691, 1545
TaiI ACGT 5 cut(s) 323, 492, 829, 894, 997
TasI AATT 6 cut(s) 98, 340, 533, 613, 694, 1211
TatI WGTACW 3 cut(s) 156, 577, 966
TauI GCSGC 1 cut(s) 595
TfiI GAWTC 2 cut(s) 392, 1552
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 3 cut(s) 1029, 1044, 1137
TspDTI ATGAA 7 cut(s) 57, 281, 353, 599, 813, 1471, 1565
TspGWI ACGGA 2 cut(s) 1437, 1587
TspRI CASTG 3 cut(s) 1029, 1044, 1137
Van91I CCANNNNNTGG 1 cut(s) 408
VpaK11BI GGWCC 3 cut(s) 313, 510, 1159
XbaI TCTAGA 1 cut(s) 1186
XcmI CCANNNNNNNNNTGG 2 cut(s) 67, 952
XmnI GAANNNNTTC 2 cut(s) 468, 1556
XspI CTAG 3 cut(s) 1187, 1260, 1295
Zsp2I ATGCAT 2 cut(s) 409, 1417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.