Rw1G014010

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004817
Physical Location & Seq
Forward (+)
31277534 .. 31286543
9010 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G014010.1

Sequence Viewer

Length: 684 bp
ATGGAACTAGGTAAGGAGTTAAGTGCAGATGGAATCTGGGCATATGATGCAACTTGGGCTCTATCAGAAGCAGTTGAGAGGACAAGAATCAAAAGTTCTACTACTGGATCCTCCAAGCATGCAATTGACCTTTTCAGAGAGATTCTGCAAACTAGATTTAAAGGTTTAAGTGGTGAAGTGATTGGCAATAAAATTTCTGCCCCCAAGGAAATCTTGGCCATAGAGGACAAAAAGACCCAAGACGATGAGGCACGTCCACCTTCCCCACCTCCAGCTAAACCAGTAAAAGTGGTAGCACCTGTAGTTGAACCACTTGATTTGCTGGGTTTGAATGATCCTGTTCCCGATACTGCAGCATTAGATGAGAAGAATGCCATGGCTTTAGCTATTGTTCCAGTGGCCAACATAATCCTTATAGATGCACCAGTTGGTGCTGGGTTTTCATACTCTACAACCTCGGAAGGGGTACAAGCTAACCAACGAGCAGGACGAGCTGGAAGGACACGTCCTGGGAAATGCTATCGGTTATATCCTTCCGTTGCTTACCATGAGGAGCTGCTGGATGCAACAATTCCTGAAATTCAACGTTCTTCACTTGCTGGAAGTGTTCTTTATTTGAAATCCTTGGACCTCCCTGATATTGACATTCTCACATTGATTTTCTTGATTCTCCTTCCTGCATAG

Protein Analysis

227

Amino Acids

24.42

Weight (kDa)

5.07

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 5 - 60 6.4e-08 Receptor family ligand binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 586
AclWI GGATC 3 cut(s) 102, 115, 329
AcoI YGGCCR 2 cut(s) 216, 399
AcsI RAATTY 2 cut(s) 192, 579
AfaI GTAC 1 cut(s) 468
AfiI CCNNNNNNNGG 1 cut(s) 462
AflIII ACRYGT 1 cut(s) 503
AgsI TTSAA 4 cut(s) 308, 331, 584, 619
AjiI CACGTC 2 cut(s) 254, 506
AjnI CCWGG 1 cut(s) 508
AluBI AGCT 5 cut(s) 275, 386, 473, 494, 556
AluI AGCT 5 cut(s) 275, 386, 473, 494, 556
AlwI GGATC 3 cut(s) 102, 115, 329
AoxI GGCC 2 cut(s) 216, 399
ApeKI GCWGC 2 cut(s) 353, 556
ApoI RAATTY 2 cut(s) 192, 579
AspS9I GGNCC 1 cut(s) 628
AsuHPI GGTGA 1 cut(s) 185
AvaII GGWCC 1 cut(s) 628
BalI TGGCCA 2 cut(s) 218, 401
BamHI GGATCC 1 cut(s) 107
BanII GRGCYC 1 cut(s) 61
BbvI GCAGC 2 cut(s) 365, 543
BccI CCATC 1 cut(s) 23
BciT130I CCWGG 1 cut(s) 510
BfaI CTAG 2 cut(s) 8, 153
BfmI CTRYAG 2 cut(s) 300, 351
BisI GCNGC 2 cut(s) 354, 557
BlsI GCNGC 2 cut(s) 355, 558
Bme1390I CCNGG 1 cut(s) 510
Bme18I GGWCC 1 cut(s) 628
BmgBI CACGTC 2 cut(s) 254, 506
BmgT120I GGNCC 1 cut(s) 628
BmiI GGNNCC 1 cut(s) 109
BmrFI CCNGG 1 cut(s) 510
BmsI GCATC 3 cut(s) 37, 409, 553
BpmI CTGGAG 1 cut(s) 255
BsaJI CCNNGG 5 cut(s) 204, 375, 456, 509, 624
Bsc4I CCNNNNNNNGG 1 cut(s) 462
Bse1I ACTGG 4 cut(s) 109, 281, 395, 425
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 5 cut(s) 204, 375, 456, 509, 624
BseGI GGATG 1 cut(s) 568
BseLI CCNNNNNNNGG 1 cut(s) 462
BseNI ACTGG 4 cut(s) 109, 281, 395, 425
BseRI GAGGAG 1 cut(s) 566
BseXI GCAGC 2 cut(s) 365, 543
BseYI CCCAGC 2 cut(s) 322, 434
BsgI GTGCAG 1 cut(s) 45
BshFI GGCC 2 cut(s) 218, 401
BslI CCNNNNNNNGG 1 cut(s) 462
BsmI GAATGC 1 cut(s) 376
BsnI GGCC 2 cut(s) 218, 401
Bsp1286I GDGCHC 1 cut(s) 61
Bsp143I GATC 2 cut(s) 107, 334
Bsp19I CCATGG 1 cut(s) 375
BspANI GGCC 2 cut(s) 218, 401
BspLI GGNNCC 1 cut(s) 109
BspMAI CTGCAG 1 cut(s) 355
BspPI GGATC 3 cut(s) 102, 115, 329
BsrI ACTGG 4 cut(s) 109, 281, 395, 425
BssECI CCNNGG 5 cut(s) 204, 375, 456, 509, 624
BssMI GATC 2 cut(s) 107, 334
BssT1I CCWWGG 3 cut(s) 204, 375, 624
Bst2UI CCWGG 1 cut(s) 510
BstAPI GCANNNNNTGC 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 120
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 1 cut(s) 568
BstKTI GATC 2 cut(s) 110, 337
BstMBI GATC 2 cut(s) 107, 334
BstMWI GCNNNNNNNGC 3 cut(s) 47, 56, 491
BstNI CCWGG 1 cut(s) 510
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 1 cut(s) 508
BstSFI CTRYAG 2 cut(s) 300, 351
BstV1I GCAGC 2 cut(s) 365, 543
BstX2I RGATCY 1 cut(s) 107
BstYI RGATCY 1 cut(s) 107
BsuRI GGCC 2 cut(s) 218, 401
BtgI CCRYGG 1 cut(s) 375
BtrI CACGTC 2 cut(s) 254, 506
BtsCI GGATG 1 cut(s) 568
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 628
Csp6I GTAC 1 cut(s) 467
CspCI CAANNNNNGTGG 2 cut(s) 300, 335
CviAII CATG 3 cut(s) 119, 376, 548
CviJI RGCY 9 cut(s) 59, 218, 275, 380, 386, 401, 473, 494, 556
CviKI_1 RGCY 9 cut(s) 59, 218, 275, 380, 386, 401, 473, 494, 556
CviQI GTAC 1 cut(s) 467
DpnI GATC 2 cut(s) 109, 336
DpnII GATC 2 cut(s) 107, 334
DraI TTTAAA 1 cut(s) 160
EaeI YGGCCR 2 cut(s) 216, 399
Eco130I CCWWGG 3 cut(s) 204, 375, 624
Eco24I GRGCYC 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 628
EcoRII CCWGG 1 cut(s) 508
EcoT14I CCWWGG 3 cut(s) 204, 375, 624
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 3 cut(s) 204, 375, 624
FaeI CATG 3 cut(s) 122, 379, 551
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 3 cut(s) 118, 375, 547
FauNDI CATATG 1 cut(s) 43
Fnu4HI GCNGC 2 cut(s) 354, 557
FokI GGATG 1 cut(s) 575
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 2 cut(s) 354, 557
FspBI CTAG 2 cut(s) 8, 153
GluI GCNGC 2 cut(s) 354, 557
GsaI CCCAGC 2 cut(s) 326, 438
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 2 cut(s) 218, 401
Hin1II CATG 3 cut(s) 122, 379, 551
HinfI GANTC 4 cut(s) 33, 87, 142, 667
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 1 cut(s) 257
Hpy188I TCNGA 3 cut(s) 67, 137, 460
Hpy188III TCNNGA 3 cut(s) 344, 575, 664
Hpy8I GTNNAC 1 cut(s) 257
HpyAV CCTTC 5 cut(s) 270, 455, 492, 543, 683
HpyCH4IV ACGT 3 cut(s) 253, 505, 586
HpyCH4V TGCA 8 cut(s) 26, 50, 122, 148, 353, 422, 566, 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 56, 491
HpySE526I ACGT 3 cut(s) 253, 505, 586
Hsp92II CATG 3 cut(s) 122, 379, 551
Kzo9I GATC 2 cut(s) 107, 334
LmnI GCTCC 1 cut(s) 553
Lsp1109I GCAGC 2 cut(s) 365, 543
LweI GCATC 3 cut(s) 37, 409, 553
MaeI CTAG 2 cut(s) 8, 153
MaeII ACGT 3 cut(s) 253, 505, 586
MalI GATC 2 cut(s) 109, 336
MboI GATC 2 cut(s) 107, 334
MboII GAAGA 2 cut(s) 379, 582
MfeI CAATTG 1 cut(s) 123
MflI RGATCY 1 cut(s) 107
MhlI GDGCHC 1 cut(s) 61
MlsI TGGCCA 2 cut(s) 218, 401
MluCI AATT 4 cut(s) 123, 192, 570, 579
MluNI TGGCCA 2 cut(s) 218, 401
MnlI CCTC 8 cut(s) 72, 121, 217, 241, 279, 466, 544, 641
Mox20I TGGCCA 2 cut(s) 218, 401
MscI TGGCCA 2 cut(s) 218, 401
MseI TTAA 3 cut(s) 20, 159, 167
Msp20I TGGCCA 2 cut(s) 218, 401
MspR9I CCNGG 1 cut(s) 510
MunI CAATTG 1 cut(s) 123
Mva1269I GAATGC 1 cut(s) 376
MvaI CCWGG 1 cut(s) 510
MwoI GCNNNNNNNGC 3 cut(s) 47, 56, 491
NcoI CCATGG 1 cut(s) 375
NdeI CATATG 1 cut(s) 43
NdeII GATC 2 cut(s) 107, 334
NlaIII CATG 3 cut(s) 122, 379, 551
NlaIV GGNNCC 1 cut(s) 109
NspI RCATGY 1 cut(s) 122
PaeI GCATGC 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 487
PctI GAATGC 1 cut(s) 376
PfeI GAWTC 4 cut(s) 33, 87, 142, 667
PkrI GCNGC 2 cut(s) 355, 558
Psp1406I AACGTT 1 cut(s) 586
Psp6I CCWGG 1 cut(s) 508
PspFI CCCAGC 2 cut(s) 322, 434
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 1 cut(s) 628
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 107
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
SaqAI TTAA 3 cut(s) 20, 159, 167
SatI GCNGC 2 cut(s) 354, 557
Sau3AI GATC 2 cut(s) 107, 334
Sau96I GGNCC 1 cut(s) 628
ScrFI CCNGG 1 cut(s) 510
SduI GDGCHC 1 cut(s) 61
SfaNI GCATC 3 cut(s) 37, 409, 553
SfcI CTRYAG 2 cut(s) 300, 351
SinI GGWCC 1 cut(s) 628
SphI GCATGC 1 cut(s) 122
Sse9I AATT 4 cut(s) 123, 192, 570, 579
SspMI CTAG 2 cut(s) 8, 153
StyD4I CCNGG 1 cut(s) 508
StyI CCWWGG 3 cut(s) 204, 375, 624
TaiI ACGT 3 cut(s) 256, 508, 589
TasI AATT 4 cut(s) 123, 192, 570, 579
TfiI GAWTC 4 cut(s) 33, 87, 142, 667
Tru1I TTAA 3 cut(s) 20, 159, 167
Tru9I TTAA 3 cut(s) 20, 159, 167
TscAI CASTG 1 cut(s) 402
TseI GCWGC 2 cut(s) 353, 556
TspDTI ATGAA 1 cut(s) 432
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 628
XapI RAATTY 2 cut(s) 192, 579
XceI RCATGY 1 cut(s) 122
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XspI CTAG 2 cut(s) 8, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.