AT5G35360

Biotin carboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004128
Physical Location & Seq
Forward (+)
13584104 .. 13588496
4393 bp
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UTR
Exon/CDS
Intron
AT5G35360.2

Sequence Viewer

Length: 1614 bp
ATGGACGCTTCTATGATTACCAATTCCAAATCCATTACTTCTCCACCCTCTTTAGCCTTAGGGAAGTCAGGAGGAGGAGGAGTTATCAGAAGTTCACTATGTAACTTAATGATGCCAAGCAAAGTTAACTTCCCTAGACAAAGAACTCAAACTCTAAAGGTTAGCCAGAAGAAACTCAAGAGAGCTACTAGTGGTGGTCTTGGTGTTACATGCAGTGGTGGTGATAAGATTCTCGTAGCGAATCGAGGTGAAATTGCAGTTCGTGTTATCAGAACTGCTCATGAAATGGGGATTCCTTGTGTTGCTGTGTATTCTACTATAGATAAAGATGCTCTTCATGTTAAATTAGCTGATGAAGCTGTTTGTATTGGTGAAGCTCCTAGTAACCAGTCGTATTTGGTGATTCCGAATGTTCTGTCTGCGGCTATTAGCCGTGGATGTACAATGCTTCATCCTGGATATGGTTTTCTTTCGGAGAACGCTCTCTTTGTTGAAATGTGTAGAGACCATGGGATCAATTTTATTGGACCTAATCCTGATAGCATCCGTGTTATGGGAGACAAAGCGACTGCAAGAGAGACAATGAAAAATGCAGGGGTTCCCACTGTACCAGGGAGTGATGGGCTATTGCAGAGTACAGAAGAAGCTGTCAGGGTCGCCAATGAGATTGGTTTCCCTGTAATGATCAAGGCAACAGCTGGTGGTGGTGGACGTGGAATGCGTCTTGCTAAGGAACCGGGAGAGTTTGTAAAACTGTTGCAGCAAGCTAAGAGCGAGGCTGCTGCTGCTTTTGGGAATGATGGATGTTATCTGGAGAAGTTCGTACAAAACCCTAGACATATTGAGTTCCAGGTGCTGGCAGATAAATTTGGAAATGTTGTTCACTTTGGTGAGCGTGACTGCAGCATCCAGAGGCGTAACCAAAAGCTTCTGGAAGAAGCACCTTCTCCAGCATTGACCGCTGAATTGCGAAAAGCCATGGGTGATGCAGCAGTCGCAGCAGCAGCATCCATTGGGTACATTGGTGTTGGTACTGTGGAGTTTCTTTTAGATGAGAGAGGTTCCTTCTACTTCATGGAAATGAACACTAGGATCCAGGTGGAGCATCCTGTGACAGAGATGATTTACTCTGTTGATTTGATAGAGGAGCAGATTCGTGTTGCAATGGGAGAGAAACTTCGTTACAAACAGGAAGATATTGTGCTCAGAGGGCACTCAATTGAATGTCGTATCAATGCAGAAGATCCATTTAAAGGATTCAGACCTGGACCTGGCAGAATAACATCATACCTGCCATCTGGAGGTCCTTTCGTTAGAATGGACAGCCATGTCTATTCCGACTATGTTGTGCCTCCAAGCTATGATTCTCTTCTTGGGAAGCTTATTGTGTGGGCTCCAACGAGGGAAAAAGCAATTGAACGGATGAAGCGGGCGCTTAATGACACTATCATTACAGGGGTTCCAACGACTATCAATTACCACAAACTTATCCTTGATGTTGAGGATTTCAAAAATGGAAAAGTTGATACAGCTTTCATCGTCAAGCATGAAGAGGAGCTAGCTGAGCCTCAAGAAATTGTGGCAGTGAAAGATCTGACAAACGCAACGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

537

Amino Acids

58.39

Weight (kDa)

6.85

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 75 - 182 4.5e-41 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 187 - 394 6.5e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 209 - 364 2.2e-11 ATP-grasp domain
Dala_Dala_lig_C PF07478 212 - 363 7.7e-06 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 408 - 490 1.8e-29 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1328
Acc36I ACCTGC 1 cut(s) 1299
AccB7I CCANNNNNTGG 1 cut(s) 856
AciI CCGC 3 cut(s) 422, 960, 1429
AclWI GGATC 4 cut(s) 521, 1087, 1100, 1238
AcsI RAATTY 1 cut(s) 866
AfaI GTAC 6 cut(s) 442, 609, 637, 825, 1019, 1033
AfiI CCNNNNNNNGG 6 cut(s) 461, 553, 856, 1253, 1271, 1301
AgsI TTSAA 4 cut(s) 494, 1223, 1418, 1510
AhlI ACTAGT 1 cut(s) 188
AjiI CACGTC 1 cut(s) 713
AjnI CCWGG 6 cut(s) 454, 610, 849, 1095, 1264, 1270
AjuI GAANNNNNNNTTGG 2 cut(s) 1348, 1380
AleI CACNNNNGTG 1 cut(s) 888
AloI GAACNNNNNNTCC 2 cut(s) 864, 896
Alw21I GWGCWC 1 cut(s) 1206
Alw26I GTCTC 3 cut(s) 498, 552, 572
AlwI GGATC 4 cut(s) 521, 1087, 1100, 1238
AlwNI CAGNNNCTG 1 cut(s) 856
ApeKI GCWGC 9 cut(s) 760, 779, 782, 785, 903, 989, 998, 1001, 1004
ApoI RAATTY 1 cut(s) 866
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AspLEI GCGC 1 cut(s) 1435
AspS9I GGNCC 3 cut(s) 527, 1268, 1304
AsuC2I CCSGG 1 cut(s) 738
AsuHPI GGTGA 6 cut(s) 233, 260, 383, 412, 902, 995
AsuNHI GCTAGC 1 cut(s) 1558
AvaII GGWCC 3 cut(s) 527, 1268, 1304
AxyI CCTNAGG 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 1215
BaeI ACNNNNGTAYC 2 cut(s) 1009, 1042
BamHI GGATCC 1 cut(s) 1092
BanII GRGCYC 1 cut(s) 1396
Bbv12I GWGCWC 1 cut(s) 1206
BbvI GCAGC 9 cut(s) 766, 769, 772, 772, 915, 1001, 1010, 1013, 1016
BccI CCATC 3 cut(s) 614, 794, 1303
BceAI ACGGC 1 cut(s) 417
BcgI CGANNNNNNTGC 2 cut(s) 764, 798
BciT130I CCWGG 6 cut(s) 456, 612, 851, 1097, 1266, 1272
BclI TGATCA 1 cut(s) 684
BcnI CCSGG 1 cut(s) 738
BcoDI GTCTC 3 cut(s) 498, 552, 572
BcuI ACTAGT 1 cut(s) 188
BfaI CTAG 6 cut(s) 135, 189, 381, 834, 1089, 1559
BfmI CTRYAG 2 cut(s) 318, 901
BfoI RGCGCY 1 cut(s) 1436
BfuAI ACCTGC 1 cut(s) 1299
BglII AGATCT 1 cut(s) 1591
BlpI GCTNAGC 1 cut(s) 1563
Bme1390I CCNGG 7 cut(s) 456, 612, 738, 851, 1097, 1266, 1272
Bme18I GGWCC 3 cut(s) 527, 1268, 1304
BmgBI CACGTC 1 cut(s) 713
BmgT120I GGNCC 3 cut(s) 527, 1268, 1304
BmiI GGNNCC 6 cut(s) 600, 735, 1063, 1094, 1395, 1461
BmrFI CCNGG 7 cut(s) 456, 612, 738, 851, 1097, 1266, 1272
BmsI GCATC 7 cut(s) 102, 319, 552, 915, 976, 1016, 1114
BmtI GCTAGC 1 cut(s) 1562
BpmI CTGGAG 3 cut(s) 833, 933, 1320
Bpu10I CCTNAGC 1 cut(s) 729
Bpu1102I GCTNAGC 1 cut(s) 1563
BpuEI CTTGAG 2 cut(s) 161, 1554
BpuMI CCSGG 1 cut(s) 738
BsaI GGTCTC 1 cut(s) 498
BsaJI CCNNGG 4 cut(s) 433, 508, 611, 978
BsaXI ACNNNNNCTCC 4 cut(s) 66, 96, 732, 762
Bsc4I CCNNNNNNNGG 6 cut(s) 461, 553, 856, 1253, 1271, 1301
Bse1I ACTGG 1 cut(s) 388
Bse21I CCTNAGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 1170
BseBI CCWGG 6 cut(s) 456, 612, 851, 1097, 1266, 1272
BseDI CCNNGG 4 cut(s) 433, 508, 611, 978
BseGI GGATG 8 cut(s) 443, 451, 543, 809, 906, 1007, 1105, 1428
BseLI CCNNNNNNNGG 6 cut(s) 461, 553, 856, 1253, 1271, 1301
BseMI GCAATG 1 cut(s) 1170
BseMII CTCAG 2 cut(s) 1219, 1554
BseNI ACTGG 1 cut(s) 388
BseRI GAGGAG 5 cut(s) 87, 90, 93, 1160, 1568
BseSI GKGCMC 1 cut(s) 1215
BseXI GCAGC 9 cut(s) 766, 769, 772, 772, 915, 1001, 1010, 1013, 1016
BsiHKAI GWGCWC 1 cut(s) 1206
BsiSI CCGG 1 cut(s) 737
BslI CCNNNNNNNGG 6 cut(s) 461, 553, 856, 1253, 1271, 1301
BsmAI GTCTC 3 cut(s) 498, 552, 572
BsmI GAATGC 1 cut(s) 723
Bso31I GGTCTC 1 cut(s) 498
Bsp1286I GDGCHC 3 cut(s) 1206, 1215, 1396
Bsp1407I TGTACA 1 cut(s) 440
Bsp143I GATC 5 cut(s) 513, 684, 1092, 1243, 1591
Bsp1720I GCTNAGC 1 cut(s) 1563
Bsp19I CCATGG 2 cut(s) 508, 978
BspACI CCGC 3 cut(s) 422, 960, 1429
BspCNI CTCAG 2 cut(s) 1218, 1555
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 6 cut(s) 600, 735, 1063, 1094, 1395, 1461
BspMAI CTGCAG 1 cut(s) 905
BspMI ACCTGC 1 cut(s) 1299
BspOI GCTAGC 1 cut(s) 1562
BspPI GGATC 4 cut(s) 521, 1087, 1100, 1238
BspQI GCTCTTC 1 cut(s) 339
BspTNI GGTCTC 1 cut(s) 498
BsrDI GCAATG 1 cut(s) 1170
BsrGI TGTACA 1 cut(s) 440
BsrI ACTGG 1 cut(s) 388
BssECI CCNNGG 4 cut(s) 433, 508, 611, 978
BssMI GATC 5 cut(s) 513, 684, 1092, 1243, 1591
BssT1I CCWWGG 2 cut(s) 508, 978
Bst2UI CCWGG 6 cut(s) 456, 612, 851, 1097, 1266, 1272
Bst4CI ACNGT 4 cut(s) 607, 756, 1036, 1609
Bst6I CTCTTC 3 cut(s) 339, 1374, 1545
BstAUI TGTACA 1 cut(s) 440
BstC8I GCNNGC 4 cut(s) 765, 858, 1431, 1560
BstDEI CTNAG 5 cut(s) 58, 729, 768, 1205, 1563
BstDSI CCRYGG 3 cut(s) 433, 508, 978
BstF5I GGATG 8 cut(s) 443, 451, 543, 809, 906, 1007, 1105, 1428
BstH2I RGCGCY 1 cut(s) 1436
BstHHI GCGC 1 cut(s) 1435
BstKTI GATC 5 cut(s) 516, 687, 1095, 1246, 1594
BstMAI GTCTC 3 cut(s) 498, 552, 572
BstMBI GATC 5 cut(s) 513, 684, 1092, 1243, 1591
BstMWI GCNNNNNNNGC 8 cut(s) 356, 785, 959, 995, 998, 1004, 1210, 1564
BstNI CCWGG 6 cut(s) 456, 612, 851, 1097, 1266, 1272
BstNSI RCATGY 1 cut(s) 213
BstSCI CCNGG 7 cut(s) 454, 610, 736, 849, 1095, 1264, 1270
BstSFI CTRYAG 2 cut(s) 318, 901
BstSLI GKGCMC 1 cut(s) 1215
BstV1I GCAGC 9 cut(s) 766, 769, 772, 772, 915, 1001, 1010, 1013, 1016
BstX2I RGATCY 3 cut(s) 1092, 1243, 1591
BstYI RGATCY 3 cut(s) 1092, 1243, 1591
Bsu36I CCTNAGG 1 cut(s) 58
BtgI CCRYGG 3 cut(s) 433, 508, 978
BtrI CACGTC 1 cut(s) 713
BtsCI GGATG 8 cut(s) 443, 451, 543, 809, 906, 1007, 1105, 1428
BtsI GCAGTG 2 cut(s) 220, 1590
BtsIMutI CAGTG 3 cut(s) 220, 603, 1590
BveI ACCTGC 1 cut(s) 1299
Cac8I GCNNGC 4 cut(s) 765, 858, 1431, 1560
CaiI CAGNNNCTG 1 cut(s) 856
CciI TCATGA 1 cut(s) 280
CfoI GCGC 1 cut(s) 1435
Cfr13I GGNCC 3 cut(s) 527, 1268, 1304
CseI GACGC 2 cut(s) 14, 710
Csp6I GTAC 6 cut(s) 441, 608, 636, 824, 1018, 1032
CviAII CATG 8 cut(s) 210, 281, 338, 509, 979, 1075, 1328, 1547
CviQI GTAC 6 cut(s) 441, 608, 636, 824, 1018, 1032
DdeI CTNAG 5 cut(s) 58, 729, 768, 1205, 1563
DpnI GATC 5 cut(s) 515, 686, 1094, 1245, 1593
DpnII GATC 5 cut(s) 513, 684, 1092, 1243, 1591
DraI TTTAAA 1 cut(s) 1252
DrdI GACNNNNNNGTC 1 cut(s) 1328
DseDI GACNNNNNNGTC 1 cut(s) 1328
Eam1104I CTCTTC 3 cut(s) 339, 1374, 1545
EarI CTCTTC 3 cut(s) 339, 1374, 1545
Eco130I CCWWGG 2 cut(s) 508, 978
Eco24I GRGCYC 1 cut(s) 1396
Eco31I GGTCTC 1 cut(s) 498
Eco47I GGWCC 3 cut(s) 527, 1268, 1304
Eco81I CCTNAGG 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 1304
EcoRII CCWGG 6 cut(s) 454, 610, 849, 1095, 1264, 1270
EcoT14I CCWWGG 2 cut(s) 508, 978
EcoT38I GRGCYC 1 cut(s) 1396
ErhI CCWWGG 2 cut(s) 508, 978
FaeI CATG 8 cut(s) 213, 284, 341, 512, 982, 1078, 1331, 1550
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FatI CATG 8 cut(s) 209, 280, 337, 508, 978, 1074, 1327, 1546
FauI CCCGC 1 cut(s) 1422
FbaI TGATCA 1 cut(s) 684
FokI GGATG 8 cut(s) 438, 450, 530, 816, 893, 994, 1092, 1435
FriOI GRGCYC 1 cut(s) 1396
FspBI CTAG 6 cut(s) 135, 189, 381, 834, 1089, 1559
GlaI GCGC 1 cut(s) 1434
GsuI CTGGAG 3 cut(s) 833, 933, 1320
HaeII RGCGCY 1 cut(s) 1436
HapII CCGG 1 cut(s) 737
HgaI GACGC 2 cut(s) 14, 710
HhaI GCGC 1 cut(s) 1435
Hin1II CATG 8 cut(s) 213, 284, 341, 512, 982, 1078, 1331, 1550
Hin6I GCGC 1 cut(s) 1433
HinP1I GCGC 1 cut(s) 1433
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 2 cut(s) 926, 1379
HinfI GANTC 7 cut(s) 229, 241, 292, 403, 1153, 1257, 1364
HpaI GTTAAC 1 cut(s) 127
HpaII CCGG 1 cut(s) 737
HphI GGTGA 6 cut(s) 233, 260, 383, 412, 902, 995
Hpy166II GTNNAC 4 cut(s) 95, 127, 710, 883
Hpy188I TCNGA 8 cut(s) 89, 272, 408, 475, 1208, 1262, 1339, 1596
Hpy188III TCNNGA 9 cut(s) 69, 178, 281, 536, 812, 910, 932, 1299, 1571
Hpy8I GTNNAC 4 cut(s) 95, 127, 710, 883
HpyAV CCTTC 2 cut(s) 954, 1075
HpyCH4III ACNGT 4 cut(s) 607, 756, 1036, 1609
HpyCH4IV ACGT 1 cut(s) 712
HpyF10VI GCNNNNNNNGC 8 cut(s) 356, 785, 959, 995, 998, 1004, 1210, 1564
HpyF3I CTNAG 5 cut(s) 58, 729, 768, 1205, 1563
HpySE526I ACGT 1 cut(s) 712
Hsp92II CATG 8 cut(s) 213, 284, 341, 512, 982, 1078, 1331, 1550
HspAI GCGC 1 cut(s) 1433
Ksp22I TGATCA 1 cut(s) 684
KspAI GTTAAC 1 cut(s) 127
Kzo9I GATC 5 cut(s) 513, 684, 1092, 1243, 1591
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 5 cut(s) 382, 1102, 1147, 1399, 1555
Lsp1109I GCAGC 9 cut(s) 766, 769, 772, 772, 915, 1001, 1010, 1013, 1016
LweI GCATC 7 cut(s) 102, 319, 552, 915, 976, 1016, 1114
MaeI CTAG 6 cut(s) 135, 189, 381, 834, 1089, 1559
MaeII ACGT 1 cut(s) 712
MaeIII GTNAC 7 cut(s) 101, 205, 383, 896, 917, 1111, 1181
MalI GATC 5 cut(s) 515, 686, 1094, 1245, 1593
MboI GATC 5 cut(s) 513, 684, 1092, 1243, 1591
MboII GAAGA 8 cut(s) 181, 326, 653, 947, 1205, 1253, 1361, 1562
MfeI CAATTG 2 cut(s) 1218, 1413
MflI RGATCY 3 cut(s) 1092, 1243, 1591
MhlI GDGCHC 3 cut(s) 1206, 1215, 1396
MmeI TCCRAC 3 cut(s) 1362, 1421, 1487
MseI TTAA 5 cut(s) 107, 126, 342, 1251, 1437
MslI CAYNNNNRTG 2 cut(s) 888, 1079
MspA1I CMGCKG 2 cut(s) 698, 962
MspI CCGG 1 cut(s) 737
MspR9I CCNGG 7 cut(s) 456, 612, 738, 851, 1097, 1266, 1272
MunI CAATTG 2 cut(s) 1218, 1413
Mva1269I GAATGC 1 cut(s) 723
MvaI CCWGG 6 cut(s) 456, 612, 851, 1097, 1266, 1272
MwoI GCNNNNNNNGC 8 cut(s) 356, 785, 959, 995, 998, 1004, 1210, 1564
NciI CCSGG 1 cut(s) 738
NcoI CCATGG 2 cut(s) 508, 978
NdeII GATC 5 cut(s) 513, 684, 1092, 1243, 1591
NheI GCTAGC 1 cut(s) 1558
NlaIII CATG 8 cut(s) 213, 284, 341, 512, 982, 1078, 1331, 1550
NlaIV GGNNCC 6 cut(s) 600, 735, 1063, 1094, 1395, 1461
NmuCI GTSAC 2 cut(s) 896, 1111
NspI RCATGY 1 cut(s) 213
OliI CACNNNNGTG 1 cut(s) 888
PagI TCATGA 1 cut(s) 280
PciSI GCTCTTC 1 cut(s) 339
PcsI WCGNNNNNNNCGW 1 cut(s) 718
PctI GAATGC 1 cut(s) 723
PfeI GAWTC 7 cut(s) 229, 241, 292, 403, 1153, 1257, 1364
PflMI CCANNNNNTGG 1 cut(s) 856
PfoI TCCNGGA 1 cut(s) 454
PpuMI RGGWCCY 1 cut(s) 1304
Psp5II RGGWCCY 1 cut(s) 1304
Psp6I CCWGG 6 cut(s) 454, 610, 849, 1095, 1264, 1270
PspGI CCWGG 6 cut(s) 454, 610, 849, 1095, 1264, 1270
PspN4I GGNNCC 6 cut(s) 600, 735, 1063, 1094, 1395, 1461
PspPI GGNCC 3 cut(s) 527, 1268, 1304
PspPPI RGGWCCY 1 cut(s) 1304
PstI CTGCAG 1 cut(s) 905
PstNI CAGNNNCTG 1 cut(s) 856
PsuI RGATCY 3 cut(s) 1092, 1243, 1591
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 6 cut(s) 442, 609, 637, 825, 1019, 1033
RsaNI GTAC 6 cut(s) 441, 608, 636, 824, 1018, 1032
RseI CAYNNNNRTG 2 cut(s) 888, 1079
SapI GCTCTTC 1 cut(s) 339
SaqAI TTAA 5 cut(s) 107, 126, 342, 1251, 1437
Sau3AI GATC 5 cut(s) 513, 684, 1092, 1243, 1591
Sau96I GGNCC 3 cut(s) 527, 1268, 1304
ScrFI CCNGG 7 cut(s) 456, 612, 738, 851, 1097, 1266, 1272
SduI GDGCHC 3 cut(s) 1206, 1215, 1396
SfaNI GCATC 7 cut(s) 102, 319, 552, 915, 976, 1016, 1114
SfcI CTRYAG 2 cut(s) 318, 901
SinI GGWCC 3 cut(s) 527, 1268, 1304
SmiMI CAYNNNNRTG 2 cut(s) 888, 1079
SmlI CTYRAG 2 cut(s) 176, 1569
SmoI CTYRAG 2 cut(s) 176, 1569
SpeI ACTAGT 1 cut(s) 188
SsiI CCGC 3 cut(s) 422, 960, 1429
SspMI CTAG 6 cut(s) 135, 189, 381, 834, 1089, 1559
StyD4I CCNGG 7 cut(s) 454, 610, 736, 849, 1095, 1264, 1270
StyI CCWWGG 2 cut(s) 508, 978
TaaI ACNGT 4 cut(s) 607, 756, 1036, 1609
TaiI ACGT 1 cut(s) 715
TaqI TCGA 1 cut(s) 244
TatI WGTACW 2 cut(s) 440, 635
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 7 cut(s) 229, 241, 292, 403, 1153, 1257, 1364
Tru1I TTAA 5 cut(s) 107, 126, 342, 1251, 1437
Tru9I TTAA 5 cut(s) 107, 126, 342, 1251, 1437
TscAI CASTG 3 cut(s) 220, 610, 1590
TseFI GTSAC 2 cut(s) 896, 1111
TseI GCWGC 9 cut(s) 760, 779, 782, 785, 903, 989, 998, 1001, 1004
Tsp45I GTSAC 2 cut(s) 896, 1111
TspGWI ACGGA 2 cut(s) 536, 1435
TspRI CASTG 3 cut(s) 220, 610, 1590
Van91I CCANNNNNTGG 1 cut(s) 856
VpaK11BI GGWCC 3 cut(s) 527, 1268, 1304
XapI RAATTY 1 cut(s) 866
XceI RCATGY 1 cut(s) 213
XspI CTAG 6 cut(s) 135, 189, 381, 834, 1089, 1559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.