MD16G1238600.v1.1

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004128
Physical Location & Seq
Reverse (-)
25177526 .. 25183747
6222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1238600.v1.1.491

Sequence Viewer

Length: 1587 bp
ATGGATGCCATAATGCCCATCTGCAAGACTGTCACGCCAACTCCGAAGGGCTTATTTTTTGGGAGAACTAGAGAGATCCGGAGCTCCCAATGTAGCTTTATGGTGGGAAATAAAGTCAACTTTTTAAGGCAGAGAGCTCCGGGTGCTCAAGTTAGTATTAAATCCAGGAGGCATGGGGGAGCTCTTCATGCTACATGTCGTGCTGAAAAAATTCTGGTGGCAAATAGAGGGGAAATTGCTGTCCGCGTTATTCGAACAGCCCATGAGATGGGAATACCTTGTGTGGCTGTTTACTCAACAATAGACAAGGATGCACTTCATGTGAAATTGGCTGATGAATCAGTTTGCATTGGTGAAGCAGCAAGCAGTCAATCGTACTTAGTTGTTCCAAACGTTTTATCTGCTGCCATCAGTAGGAAATGTACAATGTTGCATCCAGGATATGGTTTCCTTTCTGAGAATGCGTCATTTGTTGAAATGTGCAAAGAACATGGAATCAATTTTATTGGGCCTAAGCCCGAAAGCATCCGTGTTATGGGTGATAAATCGACTGCCAGAGACACAATGAAGAAAGCAGGTGTTCCAACTGTACCAGGAAGTGATGGATTACTACAGAGCACAGAGGAAGGAATCAGGCTTGCGGATGAGATTGGTTTTCCTGTTATGATCAAAGCCACAGCAGGAGGTGGAGGACGTGGTATGCGTCTTGCTAAAGAACCTGAGGAGTTTGTGAAGTTGTTACAGCAAGCAAAGAGTGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTACATCCAAAATCCTAGACACATTGAGTTTCAGGTTCTTGCAGATAAATATGGCAATGTTATTCACTTTGGAGAGCGGGATTGCAGCATCCAGAGAAGGAACCAAAAGCTCCTGGAAGAAGCACCTTCCCCTGCATTGACCCCAGAACTGCGGAAAGCTATGGGTGATGCAGCAGTTGCAGCAGCAGCATCAATTGGCTACATTGGTGTTGGAACCGTTGAATTCCTTTTGGATGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTCGGATTCAGGTTGAGCATCCTGTGACAGAAATGATTTCCTCTGTTGATTTGATTGAAGAACAAATTCGAGTAGCTATGGGTGAAAAACTCCGATACACACAGGAAGATATAGTGCTCAGAGGACATTCAATTGAATGCCGTATCAATGCAGAAGATGCTTTTAAAGGTTTCCGACCCGGGCCAGGAAGAATAACAGCCTACTTACCATCTGGAGGTCCCTTTGTTAGAATGGATAGCCATGTTTATCCTGATTACGTGGTTCCTCCAAATTACGATTCCCTTCTTGGAAAGCTTATTGTTTGGGCACCAACAAGAGAAAAGGCAATTGAGAGAATGAAGAGGGCTCTTGATGACACTGTTATAACAGGGGTTCCCACAACCATTGAATACCATAAACTTATCCTGGATATAGAGGATTTCAAAAATGGCAAGGTCGATACTGCTTTTATTCCAAAGCATGAAGAGGAGCTACAAGCGCCCCAACATTTGGTGCCTGCAGCCGCTGCCAAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

529

Amino Acids

58.0

Weight (kDa)

7.22

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 69 - 176 7.8e-42 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 181 - 388 8.4e-82 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 203 - 358 1.6e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 212 - 357 1.4e-06 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 402 - 508 2e-38 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1433
AarI CACCTGC 1 cut(s) 566
Acc36I ACCTGC 1 cut(s) 566
AccB1I GGYRCC 2 cut(s) 1375, 1561
AccB7I CCANNNNNTGG 3 cut(s) 268, 443, 1558
AccBSI CCGCTC 1 cut(s) 877
AccII CGCG 1 cut(s) 246
AccIII TCCGGA 1 cut(s) 78
AciI CCGC 5 cut(s) 244, 641, 877, 952, 1572
AclI AACGTT 1 cut(s) 393
AclWI GGATC 1 cut(s) 70
AcsI RAATTY 3 cut(s) 210, 1022, 1134
AfaI GTAC 4 cut(s) 377, 424, 591, 803
AfiI CCNNNNNNNGG 7 cut(s) 268, 414, 443, 535, 1253, 1283, 1558
AflIII ACRYGT 1 cut(s) 194
AgsI TTSAA 7 cut(s) 476, 1022, 1127, 1200, 1205, 1457, 1492
AjiI CACGTC 1 cut(s) 695
AjnI CCWGG 6 cut(s) 164, 436, 592, 912, 1252, 1473
AjuI GAANNNNNNNTTGG 2 cut(s) 1330, 1362
Alw21I GWGCWC 6 cut(s) 86, 139, 148, 184, 620, 1188
Alw26I GTCTC 1 cut(s) 552
AlwI GGATC 1 cut(s) 70
AlwNI CAGNNNCTG 1 cut(s) 1574
Ama87I CYCGRG 1 cut(s) 1247
Aor13HI TCCGGA 1 cut(s) 78
AoxI GGCC 2 cut(s) 509, 1250
ApoI RAATTY 3 cut(s) 210, 1022, 1134
Asp700I GAANNNNTTC 2 cut(s) 210, 1134
AspLEI GCGC 1 cut(s) 1549
AspS9I GGNCC 3 cut(s) 509, 1250, 1286
AsuC2I CCSGG 3 cut(s) 141, 1248, 1249
AsuHPI GGTGA 4 cut(s) 365, 551, 977, 1163
AsuII TTCGAA 1 cut(s) 253
AvaI CYCGRG 1 cut(s) 1247
AvaII GGWCC 1 cut(s) 1286
AxyI CCTNAGG 1 cut(s) 720
BaeGI GKGCMC 1 cut(s) 1378
BanI GGYRCC 2 cut(s) 1375, 1561
BanII GRGCYC 4 cut(s) 86, 139, 184, 1417
BarI GAAGNNNNNNTAC 2 cut(s) 1524, 1556
Bbv12I GWGCWC 6 cut(s) 86, 139, 148, 184, 620, 1188
BccI CCATC 6 cut(s) 26, 262, 416, 596, 776, 1285
BceAI ACGGC 1 cut(s) 1194
BciT130I CCWGG 6 cut(s) 166, 438, 594, 914, 1254, 1475
BclI TGATCA 1 cut(s) 666
BcnI CCSGG 3 cut(s) 141, 1248, 1249
BcoDI GTCTC 1 cut(s) 552
BfaI CTAG 3 cut(s) 69, 816, 1585
BfmI CTRYAG 3 cut(s) 611, 762, 1566
BfoI RGCGCY 1 cut(s) 1550
BfuAI ACCTGC 1 cut(s) 566
Bme1390I CCNGG 9 cut(s) 141, 166, 438, 594, 914, 1248, 1249, 1254, 1475
Bme18I GGWCC 1 cut(s) 1286
BmeT110I CYCGRG 1 cut(s) 1247
BmgBI CACGTC 1 cut(s) 695
BmgT120I GGNCC 3 cut(s) 509, 1250, 1286
BmiI GGNNCC 8 cut(s) 902, 1015, 1045, 1288, 1332, 1377, 1443, 1563
BmrFI CCNGG 9 cut(s) 141, 166, 438, 594, 914, 1248, 1249, 1254, 1475
BmsI GCATC 8 cut(s) 301, 442, 534, 897, 958, 998, 1096, 1216
BpmI CTGGAG 1 cut(s) 1302
Bpu10I CCTNAGC 1 cut(s) 513
Bpu14I TTCGAA 1 cut(s) 253
BpuEI CTTGAG 1 cut(s) 132
BpuMI CCSGG 3 cut(s) 141, 1248, 1249
BsaAI YACGTR 1 cut(s) 1327
BsaJI CCNNGG 1 cut(s) 1247
BsaWI WCCGGW 1 cut(s) 78
BsaXI ACNNNNNCTCC 2 cut(s) 25, 55
Bsc4I CCNNNNNNNGG 7 cut(s) 268, 414, 443, 535, 1253, 1283, 1558
Bse21I CCTNAGG 1 cut(s) 720
Bse3DI GCAATG 1 cut(s) 862
BseAI TCCGGA 1 cut(s) 78
BseBI CCWGG 6 cut(s) 166, 438, 594, 914, 1254, 1475
BseDI CCNNGG 1 cut(s) 1247
BseGI GGATG 9 cut(s) 10, 316, 433, 525, 649, 804, 888, 1039, 1087
BseLI CCNNNNNNNGG 7 cut(s) 268, 414, 443, 535, 1253, 1283, 1558
BseMI GCAATG 1 cut(s) 862
BseMII CTCAG 3 cut(s) 447, 711, 1201
BseRI GAGGAG 2 cut(s) 737, 1550
BseSI GKGCMC 1 cut(s) 1378
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 2 cut(s) 511, 1252
BshNI GGYRCC 2 cut(s) 1375, 1561
BsiHKAI GWGCWC 6 cut(s) 86, 139, 148, 184, 620, 1188
BsiHKCI CYCGRG 1 cut(s) 1247
BsiSI CCGG 3 cut(s) 79, 140, 1248
BslFI GGGAC 1 cut(s) 1272
BslI CCNNNNNNNGG 7 cut(s) 268, 414, 443, 535, 1253, 1283, 1558
BsmAI GTCTC 1 cut(s) 552
BsmFI GGGAC 1 cut(s) 1272
BsmI GAATGC 2 cut(s) 466, 1211
BsnI GGCC 2 cut(s) 511, 1252
BsoBI CYCGRG 1 cut(s) 1247
Bsp119I TTCGAA 1 cut(s) 253
Bsp1286I GDGCHC 8 cut(s) 86, 139, 148, 184, 620, 1188, 1378, 1417
Bsp13I TCCGGA 1 cut(s) 78
Bsp1407I TGTACA 1 cut(s) 422
Bsp143I GATC 2 cut(s) 75, 666
BspACI CCGC 5 cut(s) 244, 641, 877, 952, 1572
BspANI GGCC 2 cut(s) 511, 1252
BspCNI CTCAG 3 cut(s) 448, 712, 1200
BspEI TCCGGA 1 cut(s) 78
BspFNI CGCG 1 cut(s) 246
BspLI GGNNCC 8 cut(s) 902, 1015, 1045, 1288, 1332, 1377, 1443, 1563
BspMAI CTGCAG 2 cut(s) 766, 1570
BspMI ACCTGC 1 cut(s) 566
BspPI GGATC 1 cut(s) 70
BspQI GCTCTTC 1 cut(s) 189
BspT104I TTCGAA 1 cut(s) 253
BspT107I GGYRCC 2 cut(s) 1375, 1561
BsrBI CCGCTC 1 cut(s) 877
BsrDI GCAATG 1 cut(s) 862
BsrGI TGTACA 1 cut(s) 422
BssECI CCNNGG 1 cut(s) 1247
BssMI GATC 2 cut(s) 75, 666
Bst2UI CCWGG 6 cut(s) 166, 438, 594, 914, 1254, 1475
Bst4CI ACNGT 4 cut(s) 31, 589, 1018, 1429
Bst6I CTCTTC 3 cut(s) 189, 1403, 1527
BstAPI GCANNNNNTGC 3 cut(s) 977, 1226, 1574
BstAUI TGTACA 1 cut(s) 422
BstBAI YACGTR 1 cut(s) 1327
BstBI TTCGAA 1 cut(s) 253
BstC8I GCNNGC 4 cut(s) 364, 639, 747, 1566
BstDEI CTNAG 5 cut(s) 379, 456, 513, 720, 1187
BstF5I GGATG 9 cut(s) 10, 316, 433, 525, 649, 804, 888, 1039, 1087
BstFNI CGCG 1 cut(s) 246
BstH2I RGCGCY 1 cut(s) 1550
BstHHI GCGC 1 cut(s) 1549
BstKTI GATC 2 cut(s) 78, 669
BstMAI GTCTC 1 cut(s) 552
BstMBI GATC 2 cut(s) 75, 666
BstMWI GCNNNNNNNGC 9 cut(s) 143, 188, 767, 977, 980, 986, 1226, 1546, 1574
BstNI CCWGG 6 cut(s) 166, 438, 594, 914, 1254, 1475
BstNSI RCATGY 1 cut(s) 198
BstSCI CCNGG 9 cut(s) 139, 164, 436, 592, 912, 1246, 1247, 1252, 1473
BstSFI CTRYAG 3 cut(s) 611, 762, 1566
BstSLI GKGCMC 1 cut(s) 1378
BstUI CGCG 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
Bsu36I CCTNAGG 1 cut(s) 720
BsuRI GGCC 2 cut(s) 511, 1252
BtrI CACGTC 1 cut(s) 695
BtsCI GGATG 9 cut(s) 10, 316, 433, 525, 649, 804, 888, 1039, 1087
BtsIMutI CAGTG 1 cut(s) 1425
BveI ACCTGC 1 cut(s) 566
Cac8I GCNNGC 4 cut(s) 364, 639, 747, 1566
CaiI CAGNNNCTG 1 cut(s) 1574
CfoI GCGC 1 cut(s) 1549
Cfr13I GGNCC 3 cut(s) 509, 1250, 1286
Cfr9I CCCGGG 1 cut(s) 1247
CseI GACGC 2 cut(s) 453, 692
Csp6I GTAC 4 cut(s) 376, 423, 590, 802
CspCI CAANNNNNGTGG 2 cut(s) 1435, 1470
CviAII CATG 9 cut(s) 173, 188, 195, 263, 320, 491, 1057, 1310, 1529
CviQI GTAC 4 cut(s) 376, 423, 590, 802
DdeI CTNAG 5 cut(s) 379, 456, 513, 720, 1187
DpnI GATC 2 cut(s) 77, 668
DpnII GATC 2 cut(s) 75, 666
DraI TTTAAA 1 cut(s) 1234
Eam1104I CTCTTC 3 cut(s) 189, 1403, 1527
EarI CTCTTC 3 cut(s) 189, 1403, 1527
Ecl136II GAGCTC 3 cut(s) 84, 137, 182
Eco24I GRGCYC 4 cut(s) 86, 139, 184, 1417
Eco47I GGWCC 1 cut(s) 1286
Eco53kI GAGCTC 3 cut(s) 84, 137, 182
Eco81I CCTNAGG 1 cut(s) 720
Eco88I CYCGRG 1 cut(s) 1247
EcoICRI GAGCTC 3 cut(s) 84, 137, 182
EcoO109I RGGNCCY 1 cut(s) 1286
EcoRI GAATTC 1 cut(s) 1022
EcoRII CCWGG 6 cut(s) 164, 436, 592, 912, 1252, 1473
EcoT38I GRGCYC 4 cut(s) 86, 139, 184, 1417
FaeI CATG 9 cut(s) 176, 191, 198, 266, 323, 494, 1060, 1313, 1532
FaqI GGGAC 1 cut(s) 1272
FatI CATG 9 cut(s) 172, 187, 194, 262, 319, 490, 1056, 1309, 1528
FauI CCCGC 1 cut(s) 870
FbaI TGATCA 1 cut(s) 666
FokI GGATG 9 cut(s) 17, 323, 420, 512, 656, 791, 875, 1046, 1074
FriOI GRGCYC 4 cut(s) 86, 139, 184, 1417
FspBI CTAG 3 cut(s) 69, 816, 1585
GlaI GCGC 1 cut(s) 1548
GsuI CTGGAG 1 cut(s) 1302
HaeII RGCGCY 1 cut(s) 1550
HaeIII GGCC 2 cut(s) 511, 1252
HapII CCGG 3 cut(s) 79, 140, 1248
HgaI GACGC 2 cut(s) 453, 692
HhaI GCGC 1 cut(s) 1549
Hin1II CATG 9 cut(s) 176, 191, 198, 266, 323, 494, 1060, 1313, 1532
Hin6I GCGC 1 cut(s) 1547
HinP1I GCGC 1 cut(s) 1547
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 1361
HinfI GANTC 5 cut(s) 338, 495, 630, 1075, 1346
HpaII CCGG 3 cut(s) 79, 140, 1248
HphI GGTGA 4 cut(s) 365, 551, 977, 1163
Hpy166II GTNNAC 2 cut(s) 118, 292
Hpy188I TCNGA 6 cut(s) 45, 457, 1074, 1163, 1190, 1244
Hpy188III TCNNGA 5 cut(s) 79, 892, 1281, 1319, 1418
Hpy8I GTNNAC 2 cut(s) 118, 292
HpyAV CCTTC 5 cut(s) 40, 620, 891, 936, 1361
HpyCH4III ACNGT 4 cut(s) 31, 589, 1018, 1429
HpyCH4IV ACGT 3 cut(s) 393, 694, 1326
HpyF10VI GCNNNNNNNGC 9 cut(s) 143, 188, 767, 977, 980, 986, 1226, 1546, 1574
HpyF3I CTNAG 5 cut(s) 379, 456, 513, 720, 1187
HpySE526I ACGT 3 cut(s) 393, 694, 1326
Hsp92II CATG 9 cut(s) 176, 191, 198, 266, 323, 494, 1060, 1313, 1532
HspAI GCGC 1 cut(s) 1547
Kpn2I TCCGGA 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 666
Kzo9I GATC 2 cut(s) 75, 666
LguI GCTCTTC 1 cut(s) 189
LmnI GCTCC 6 cut(s) 81, 89, 142, 179, 915, 1537
LweI GCATC 8 cut(s) 301, 442, 534, 897, 958, 998, 1096, 1216
MaeI CTAG 3 cut(s) 69, 816, 1585
MaeII ACGT 3 cut(s) 393, 694, 1326
MaeIII GTNAC 3 cut(s) 31, 738, 1093
MalI GATC 2 cut(s) 77, 668
MbiI CCGCTC 1 cut(s) 877
MboI GATC 2 cut(s) 75, 666
MboII GAAGA 9 cut(s) 176, 580, 929, 1139, 1187, 1235, 1269, 1420, 1544
MfeI CAATTG 3 cut(s) 993, 1200, 1395
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 8 cut(s) 86, 139, 148, 184, 620, 1188, 1378, 1417
MmeI TCCRAC 3 cut(s) 608, 991, 1267
MroI TCCGGA 1 cut(s) 78
MroXI GAANNNNTTC 2 cut(s) 210, 1134
MseI TTAA 3 cut(s) 125, 159, 1233
MslI CAYNNNNRTG 1 cut(s) 1061
MspA1I CMGCKG 2 cut(s) 767, 1574
MspI CCGG 3 cut(s) 79, 140, 1248
MspR9I CCNGG 9 cut(s) 141, 166, 438, 594, 914, 1248, 1249, 1254, 1475
MunI CAATTG 3 cut(s) 993, 1200, 1395
Mva1269I GAATGC 2 cut(s) 466, 1211
MvaI CCWGG 6 cut(s) 166, 438, 594, 914, 1254, 1475
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 9 cut(s) 143, 188, 767, 977, 980, 986, 1226, 1546, 1574
NciI CCSGG 3 cut(s) 141, 1248, 1249
NdeII GATC 2 cut(s) 75, 666
NlaIII CATG 9 cut(s) 176, 191, 198, 266, 323, 494, 1060, 1313, 1532
NlaIV GGNNCC 8 cut(s) 902, 1015, 1045, 1288, 1332, 1377, 1443, 1563
NmuCI GTSAC 2 cut(s) 31, 1093
NspI RCATGY 1 cut(s) 198
NspV TTCGAA 1 cut(s) 253
PaqCI CACCTGC 1 cut(s) 566
PciI ACATGT 1 cut(s) 194
PciSI GCTCTTC 1 cut(s) 189
PcsI WCGNNNNNNNCGW 2 cut(s) 41, 700
PctI GAATGC 2 cut(s) 466, 1211
PdmI GAANNNNTTC 2 cut(s) 210, 1134
PfeI GAWTC 5 cut(s) 338, 495, 630, 1075, 1346
PflMI CCANNNNNTGG 3 cut(s) 268, 443, 1558
PfoI TCCNGGA 4 cut(s) 164, 436, 912, 1473
Ppu21I YACGTR 1 cut(s) 1327
PpuMI RGGWCCY 1 cut(s) 1286
PscI ACATGT 1 cut(s) 194
PsiI TTATAA 1 cut(s) 1433
Psp124BI GAGCTC 3 cut(s) 86, 139, 184
Psp1406I AACGTT 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 1286
Psp6I CCWGG 6 cut(s) 164, 436, 592, 912, 1252, 1473
PspGI CCWGG 6 cut(s) 164, 436, 592, 912, 1252, 1473
PspN4I GGNNCC 8 cut(s) 902, 1015, 1045, 1288, 1332, 1377, 1443, 1563
PspPI GGNCC 3 cut(s) 509, 1250, 1286
PspPPI RGGWCCY 1 cut(s) 1286
PstI CTGCAG 2 cut(s) 766, 1570
PstNI CAGNNNCTG 1 cut(s) 1574
PsuI RGATCY 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 767
RsaI GTAC 4 cut(s) 377, 424, 591, 803
RsaNI GTAC 4 cut(s) 376, 423, 590, 802
RseI CAYNNNNRTG 1 cut(s) 1061
SacI GAGCTC 3 cut(s) 86, 139, 184
SapI GCTCTTC 1 cut(s) 189
SaqAI TTAA 3 cut(s) 125, 159, 1233
Sau3AI GATC 2 cut(s) 75, 666
Sau96I GGNCC 3 cut(s) 509, 1250, 1286
ScrFI CCNGG 9 cut(s) 141, 166, 438, 594, 914, 1248, 1249, 1254, 1475
SduI GDGCHC 8 cut(s) 86, 139, 148, 184, 620, 1188, 1378, 1417
SfaNI GCATC 8 cut(s) 301, 442, 534, 897, 958, 998, 1096, 1216
SfcI CTRYAG 3 cut(s) 611, 762, 1566
SfuI TTCGAA 1 cut(s) 253
SinI GGWCC 1 cut(s) 1286
SmaI CCCGGG 1 cut(s) 1249
SmiMI CAYNNNNRTG 1 cut(s) 1061
SmlI CTYRAG 1 cut(s) 147
SmoI CTYRAG 1 cut(s) 147
SsiI CCGC 5 cut(s) 244, 641, 877, 952, 1572
SspMI CTAG 3 cut(s) 69, 816, 1585
SstI GAGCTC 3 cut(s) 86, 139, 184
StyD4I CCNGG 9 cut(s) 139, 164, 436, 592, 912, 1246, 1247, 1252, 1473
TaaI ACNGT 4 cut(s) 31, 589, 1018, 1429
TaiI ACGT 3 cut(s) 396, 697, 1329
TaqI TCGA 4 cut(s) 253, 548, 1138, 1506
TatI WGTACW 2 cut(s) 422, 801
TauI GCSGC 1 cut(s) 1574
TfiI GAWTC 5 cut(s) 338, 495, 630, 1075, 1346
Tru1I TTAA 3 cut(s) 125, 159, 1233
Tru9I TTAA 3 cut(s) 125, 159, 1233
TscAI CASTG 1 cut(s) 1432
TseFI GTSAC 2 cut(s) 31, 1093
Tsp45I GTSAC 2 cut(s) 31, 1093
TspDTI ATGAA 9 cut(s) 176, 308, 351, 581, 1045, 1050, 1079, 1421, 1545
TspGWI ACGGA 1 cut(s) 518
TspMI CCCGGG 1 cut(s) 1247
TspRI CASTG 1 cut(s) 1432
Van91I CCANNNNNTGG 3 cut(s) 268, 443, 1558
VpaK11BI GGWCC 1 cut(s) 1286
XapI RAATTY 3 cut(s) 210, 1022, 1134
XceI RCATGY 1 cut(s) 198
XmaI CCCGGG 1 cut(s) 1247
XmnI GAANNNNTTC 2 cut(s) 210, 1134
XspI CTAG 3 cut(s) 69, 816, 1585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.