pycom16g19960

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004128
Physical Location & Seq
Reverse (-)
17416693 .. 17423302
6610 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g19960.12

Sequence Viewer

Length: 1812 bp
ATGCTCTCCTCCGTCAGCCGTCCTCCACTCCTCTTCCTCTCATCACCTCCCAGCTCGCCCCACTCCTTCTTCATTGCCACGGTCAACAGCAATAGTAACCTAAAACCATTCAGTGACTGGGATATTTGGTGTAACACATATCGCTTTGTTTTGAAAATGGATGCCATAATGCCCATCTGCAAGACTGTCACGCCAACTCCGGGCTTATTTTTTGGGAGAACTAGAGAGATCCGGAGCTCCCAATGTAGCTTTATGGTGGGAAATAAAGTCAACTTTTTAAGGCAGAGAGCTCAGGGTGCTCAAGTTAGTATTAAATCCAGGAGGCATGGGGGAGCTCTTCATGCTACATGTCGTGCTGAAAAAATTCTGGTGGCAAATAGAGGGGAAATTGCCGTCCGCGTTATTCGAACAGCCCATGAGATGGGAATACCTTGTGTGGCTGTTTACTCAACAAGAGACAAGGATGCACTTCATGTGAAATTGGCTGATGAATCAGTTTGCATTGGTGAAGCAGCAAGCAGTCAATCGTACTTAGTTGTTCCAAACGTTTTATCTGCTGCCATCAGTCGGAAATGTACAATGTTGCATCCGGGATATGGTTTCCTTTCTGAGAATGCATCATTTGTTGAAATGTGCAAAGAACATGGAATCAATTTTATTGGGCCTAAGCCCGAAAGCATCCGTGTTATGGGTGATAAATCAACTGCCAGAAACACAATGAAGAAAGCAGGTGTTCCAACTGTACCAGGAAGTGATGGATTACTACAGAGCACAGAGGAAGGAATCAGGCTTGCGGATGAGATTGGTTTTCCTGTTATGATCAAAGCCACAGCAGGAGGTGGAGGACGTGGTATGCGTCTTGCTAAAGAACCTGAGGAGTTTGTGAAGTTGTTACAGCAAGCAAAGAGTGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTACATCCAAAATCCTAGACACATTGAGTTTCAGGTTCTTGCAGATAAATATGGCAGTGTTATTCACTTTGGAGAGCGGGATTGCAGCATCCAGAGAAGGAACCAAAAGCTTCTGGAAGAAGCACCTTCCCCAGCATTGACCCCAGAACTGCGGAAAGCTATGGGTGATGCAGCAGTTGCAGCAGCAGCATCGATTGGCTACATTGGTGTTGGAACCGTTGAATTCCTTTTGGATGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTCGGATTCAGGTTGAGCATCCTGTGACAGAAATGATTTCCTCTGTTGACTTGATTGAAGAACAAATTCGTGTGGCTATGGGTGAAAAACTCCGATACACACAGGAAGATATAGTGCTCAGAGGACATTCAATTGAATGCCGTATCAATGCAGAAGACGCTTTTAAAGGATTCCGACCTGGGCCAGGAAGAATAACAGCCTACTTACCATCTGGAGGTCCCTTTGTTAGAATGGATAGCCATGTTTATCCTGATTATGTGGTTCCTCCAAATTACGATTCCCTTCTTGGAAAGCTTATTGTTTGGGCACCAACAAGAGAAAAGGCAATTGCGCGAATGAAGAGGGCTCTTGACGACACTGTCATAACAGGGGTTCCCACAACCATTGAATACCATAAACTTATCCTTGATATAGAGGATTTCAAAAATGGCAACGTCGATACTGCTTTTATTCCAAAGCATGAAGAGGAGCTACAAGCGGTAAGAACCCCAACATTTGGTGCCTGCAGCCGCTGCCAAGAACTAGCCGGTGCATCTGCTTACATGGATGGCTTTCGAATTGCTACTATCTGGTCTACTCGACAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

604

Amino Acids

66.53

Weight (kDa)

8.34

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 719
AasI GACNNNNNNGTC 1 cut(s) 1580
Acc36I ACCTGC 1 cut(s) 719
AccB1I GGYRCC 2 cut(s) 1528, 1721
AccB7I CCANNNNNTGG 2 cut(s) 421, 1718
AccBSI CCGCTC 1 cut(s) 1030
AccI GTMKAC 1 cut(s) 1796
AccII CGCG 2 cut(s) 399, 1555
AccIII TCCGGA 1 cut(s) 231
AciI CCGC 6 cut(s) 397, 794, 1030, 1105, 1700, 1732
AclI AACGTT 1 cut(s) 546
AclWI GGATC 1 cut(s) 223
AcsI RAATTY 3 cut(s) 363, 1175, 1287
AfaI GTAC 4 cut(s) 530, 577, 744, 956
AfiI CCNNNNNNNGG 8 cut(s) 200, 421, 567, 596, 688, 1406, 1436, 1718
AflIII ACRYGT 1 cut(s) 347
AgsI TTSAA 8 cut(s) 154, 629, 1175, 1280, 1353, 1358, 1610, 1645
AjiI CACGTC 1 cut(s) 848
AjnI CCWGG 4 cut(s) 317, 745, 1399, 1405
AjuI GAANNNNNNNTTGG 2 cut(s) 1483, 1515
Alw21I GWGCWC 6 cut(s) 239, 292, 301, 337, 773, 1341
Alw26I GTCTC 1 cut(s) 450
AlwI GGATC 1 cut(s) 223
AlwNI CAGNNNCTG 2 cut(s) 117, 1734
Aor13HI TCCGGA 1 cut(s) 231
AoxI GGCC 2 cut(s) 662, 1403
ApoI RAATTY 3 cut(s) 363, 1175, 1287
Asp700I GAANNNNTTC 2 cut(s) 363, 1287
AspLEI GCGC 1 cut(s) 1555
AspS9I GGNCC 3 cut(s) 662, 1403, 1439
AsuC2I CCSGG 2 cut(s) 201, 591
AsuHPI GGTGA 5 cut(s) 36, 518, 704, 1130, 1316
AsuII TTCGAA 2 cut(s) 406, 1777
AvaII GGWCC 1 cut(s) 1439
AxyI CCTNAGG 1 cut(s) 873
BaeGI GKGCMC 1 cut(s) 1531
BanI GGYRCC 2 cut(s) 1528, 1721
BanII GRGCYC 4 cut(s) 239, 292, 337, 1570
BarI GAAGNNNNNNTAC 2 cut(s) 1677, 1709
BbsI GAAGAC 1 cut(s) 1383
Bbv12I GWGCWC 6 cut(s) 239, 292, 301, 337, 773, 1341
BccI CCATC 7 cut(s) 182, 415, 569, 749, 929, 1438, 1763
BceAI ACGGC 3 cut(s) 3, 377, 1347
BcgI CGANNNNNNTGC 2 cut(s) 1125, 1159
BciT130I CCWGG 4 cut(s) 319, 747, 1401, 1407
BclI TGATCA 1 cut(s) 819
BcnI CCSGG 2 cut(s) 201, 591
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 3 cut(s) 222, 969, 1745
BfmI CTRYAG 3 cut(s) 764, 915, 1726
BfuAI ACCTGC 1 cut(s) 719
Bme1390I CCNGG 6 cut(s) 201, 319, 591, 747, 1401, 1407
Bme18I GGWCC 1 cut(s) 1439
BmgBI CACGTC 1 cut(s) 848
BmgT120I GGNCC 3 cut(s) 662, 1403, 1439
BmiI GGNNCC 8 cut(s) 1055, 1168, 1198, 1441, 1485, 1530, 1596, 1723
BmrFI CCNGG 6 cut(s) 201, 319, 591, 747, 1401, 1407
BmrI ACTGGG 1 cut(s) 127
BmuI ACTGGG 1 cut(s) 127
BpiI GAAGAC 1 cut(s) 1383
BpmI CTGGAG 1 cut(s) 1455
Bpu10I CCTNAGC 2 cut(s) 291, 666
Bpu14I TTCGAA 2 cut(s) 406, 1777
BpuEI CTTGAG 1 cut(s) 285
BpuMI CCSGG 2 cut(s) 201, 591
Bsa29I ATCGAT 1 cut(s) 1145
BsaJI CCNNGG 2 cut(s) 78, 1400
BsaWI WCCGGW 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 8 cut(s) 200, 421, 567, 596, 688, 1406, 1436, 1718
Bse118I RCCGGY 1 cut(s) 1748
Bse1I ACTGG 1 cut(s) 122
Bse21I CCTNAGG 1 cut(s) 873
Bse3DI GCAATG 1 cut(s) 72
BseAI TCCGGA 1 cut(s) 231
BseBI CCWGG 4 cut(s) 319, 747, 1401, 1407
BseCI ATCGAT 1 cut(s) 1145
BseDI CCNNGG 2 cut(s) 78, 1400
BseLI CCNNNNNNNGG 8 cut(s) 200, 421, 567, 596, 688, 1406, 1436, 1718
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 4 cut(s) 305, 600, 864, 1354
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 3 cut(s) 20, 890, 1703
BseSI GKGCMC 1 cut(s) 1531
BseYI CCCAGC 2 cut(s) 50, 1084
Bsh1236I CGCG 2 cut(s) 399, 1555
BshFI GGCC 2 cut(s) 664, 1405
BshNI GGYRCC 2 cut(s) 1528, 1721
BshVI ATCGAT 1 cut(s) 1145
BsiHKAI GWGCWC 6 cut(s) 239, 292, 301, 337, 773, 1341
BsiSI CCGG 4 cut(s) 200, 232, 590, 1749
BslFI GGGAC 1 cut(s) 1425
BslI CCNNNNNNNGG 8 cut(s) 200, 421, 567, 596, 688, 1406, 1436, 1718
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 1425
BsmI GAATGC 2 cut(s) 619, 1364
BsnI GGCC 2 cut(s) 664, 1405
Bsp119I TTCGAA 2 cut(s) 406, 1777
Bsp1286I GDGCHC 8 cut(s) 239, 292, 301, 337, 773, 1341, 1531, 1570
Bsp13I TCCGGA 1 cut(s) 231
Bsp1407I TGTACA 1 cut(s) 575
Bsp143I GATC 2 cut(s) 228, 819
BspACI CCGC 6 cut(s) 397, 794, 1030, 1105, 1700, 1732
BspANI GGCC 2 cut(s) 664, 1405
BspCNI CTCAG 4 cut(s) 304, 601, 865, 1353
BspDI ATCGAT 1 cut(s) 1145
BspEI TCCGGA 1 cut(s) 231
BspFNI CGCG 2 cut(s) 399, 1555
BspLI GGNNCC 8 cut(s) 1055, 1168, 1198, 1441, 1485, 1530, 1596, 1723
BspMAI CTGCAG 2 cut(s) 919, 1730
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 1 cut(s) 223
BspQI GCTCTTC 1 cut(s) 342
BspT104I TTCGAA 2 cut(s) 406, 1777
BspT107I GGYRCC 2 cut(s) 1528, 1721
BsrBI CCGCTC 1 cut(s) 1030
BsrDI GCAATG 1 cut(s) 72
BsrFI RCCGGY 1 cut(s) 1748
BsrGI TGTACA 1 cut(s) 575
BsrI ACTGG 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 1748
BssECI CCNNGG 2 cut(s) 78, 1400
BssMI GATC 2 cut(s) 228, 819
Bst2UI CCWGG 4 cut(s) 319, 747, 1401, 1407
Bst4CI ACNGT 5 cut(s) 82, 187, 742, 1171, 1582
Bst6I CTCTTC 4 cut(s) 38, 342, 1556, 1680
BstAPI GCANNNNNTGC 2 cut(s) 1130, 1734
BstAUI TGTACA 1 cut(s) 575
BstBI TTCGAA 2 cut(s) 406, 1777
BstC8I GCNNGC 5 cut(s) 56, 517, 792, 900, 1726
BstDEI CTNAG 6 cut(s) 291, 532, 609, 666, 873, 1340
BstDSI CCRYGG 1 cut(s) 78
BstENI CCTNNNNNAGG 1 cut(s) 1404
BstFNI CGCG 2 cut(s) 399, 1555
BstHHI GCGC 1 cut(s) 1555
BstKTI GATC 2 cut(s) 231, 822
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 2 cut(s) 228, 819
BstMWI GCNNNNNNNGC 8 cut(s) 296, 341, 920, 1130, 1133, 1139, 1379, 1734
BstNI CCWGG 4 cut(s) 319, 747, 1401, 1407
BstNSI RCATGY 1 cut(s) 351
BstSCI CCNGG 6 cut(s) 199, 317, 589, 745, 1399, 1405
BstSFI CTRYAG 3 cut(s) 764, 915, 1726
BstSLI GKGCMC 1 cut(s) 1531
BstUI CGCG 2 cut(s) 399, 1555
BstV2I GAAGAC 1 cut(s) 1383
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 1145
Bsu36I CCTNAGG 1 cut(s) 873
BsuRI GGCC 2 cut(s) 664, 1405
BsuTUI ATCGAT 1 cut(s) 1145
BtgI CCRYGG 1 cut(s) 78
BtrI CACGTC 1 cut(s) 848
BtsI GCAGTG 1 cut(s) 1015
BtsIMutI CAGTG 3 cut(s) 118, 1015, 1578
BveI ACCTGC 1 cut(s) 719
Cac8I GCNNGC 5 cut(s) 56, 517, 792, 900, 1726
CaiI CAGNNNCTG 2 cut(s) 117, 1734
CfoI GCGC 1 cut(s) 1555
Cfr10I RCCGGY 1 cut(s) 1748
Cfr13I GGNCC 3 cut(s) 662, 1403, 1439
ClaI ATCGAT 1 cut(s) 1145
CseI GACGC 2 cut(s) 845, 1388
Csp6I GTAC 4 cut(s) 529, 576, 743, 955
CspCI CAANNNNNGTGG 2 cut(s) 1588, 1623
CviQI GTAC 4 cut(s) 529, 576, 743, 955
DdeI CTNAG 6 cut(s) 291, 532, 609, 666, 873, 1340
DpnI GATC 2 cut(s) 230, 821
DpnII GATC 2 cut(s) 228, 819
DraI TTTAAA 1 cut(s) 1387
DrdI GACNNNNNNGTC 1 cut(s) 1580
DseDI GACNNNNNNGTC 1 cut(s) 1580
Eam1104I CTCTTC 4 cut(s) 38, 342, 1556, 1680
EarI CTCTTC 4 cut(s) 38, 342, 1556, 1680
Ecl136II GAGCTC 3 cut(s) 237, 290, 335
Eco24I GRGCYC 4 cut(s) 239, 292, 337, 1570
Eco47I GGWCC 1 cut(s) 1439
Eco53kI GAGCTC 3 cut(s) 237, 290, 335
Eco81I CCTNAGG 1 cut(s) 873
EcoICRI GAGCTC 3 cut(s) 237, 290, 335
EcoNI CCTNNNNNAGG 1 cut(s) 1404
EcoO109I RGGNCCY 1 cut(s) 1439
EcoRI GAATTC 1 cut(s) 1175
EcoRII CCWGG 4 cut(s) 317, 745, 1399, 1405
EcoT22I ATGCAT 1 cut(s) 619
EcoT38I GRGCYC 4 cut(s) 239, 292, 337, 1570
FaqI GGGAC 1 cut(s) 1425
FauI CCCGC 1 cut(s) 1023
FbaI TGATCA 1 cut(s) 819
FblI GTMKAC 1 cut(s) 1796
FriOI GRGCYC 4 cut(s) 239, 292, 337, 1570
FspBI CTAG 3 cut(s) 222, 969, 1745
GlaI GCGC 1 cut(s) 1554
GsaI CCCAGC 2 cut(s) 54, 1088
GsuI CTGGAG 1 cut(s) 1455
HaeIII GGCC 2 cut(s) 664, 1405
HapII CCGG 4 cut(s) 200, 232, 590, 1749
HgaI GACGC 2 cut(s) 845, 1388
HhaI GCGC 1 cut(s) 1555
Hin6I GCGC 1 cut(s) 1553
HinP1I GCGC 1 cut(s) 1553
HincII GTYRAC 3 cut(s) 85, 271, 1270
HindII GTYRAC 3 cut(s) 85, 271, 1270
HindIII AAGCTT 2 cut(s) 1061, 1514
HinfI GANTC 6 cut(s) 491, 648, 783, 1228, 1392, 1499
HpaII CCGG 4 cut(s) 200, 232, 590, 1749
HphI GGTGA 5 cut(s) 36, 518, 704, 1130, 1316
Hpy166II GTNNAC 5 cut(s) 85, 271, 445, 1270, 1797
Hpy188I TCNGA 6 cut(s) 570, 610, 1227, 1316, 1343, 1397
Hpy188III TCNNGA 6 cut(s) 232, 1045, 1067, 1434, 1472, 1571
Hpy8I GTNNAC 5 cut(s) 85, 271, 445, 1270, 1797
Hpy99I CGWCG 1 cut(s) 1661
HpyAV CCTTC 5 cut(s) 76, 773, 1044, 1089, 1514
HpyCH4III ACNGT 5 cut(s) 82, 187, 742, 1171, 1582
HpyCH4IV ACGT 3 cut(s) 546, 847, 1656
HpyF10VI GCNNNNNNNGC 8 cut(s) 296, 341, 920, 1130, 1133, 1139, 1379, 1734
HpyF3I CTNAG 6 cut(s) 291, 532, 609, 666, 873, 1340
HpySE526I ACGT 3 cut(s) 546, 847, 1656
HspAI GCGC 1 cut(s) 1553
Kpn2I TCCGGA 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 819
Kzo9I GATC 2 cut(s) 228, 819
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 4 cut(s) 234, 242, 332, 1690
MaeI CTAG 3 cut(s) 222, 969, 1745
MaeII ACGT 3 cut(s) 546, 847, 1656
MaeIII GTNAC 6 cut(s) 95, 113, 131, 187, 891, 1246
MalI GATC 2 cut(s) 230, 821
MbiI CCGCTC 1 cut(s) 1030
MboI GATC 2 cut(s) 228, 819
MfeI CAATTG 2 cut(s) 1353, 1548
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 8 cut(s) 239, 292, 301, 337, 773, 1341, 1531, 1570
MmeI TCCRAC 4 cut(s) 548, 761, 1144, 1420
Mph1103I ATGCAT 1 cut(s) 619
MroI TCCGGA 1 cut(s) 231
MroXI GAANNNNTTC 2 cut(s) 363, 1287
MseI TTAA 4 cut(s) 278, 312, 1386, 1810
MslI CAYNNNNRTG 1 cut(s) 1214
MspA1I CMGCKG 2 cut(s) 920, 1734
MspI CCGG 4 cut(s) 200, 232, 590, 1749
MspR9I CCNGG 6 cut(s) 201, 319, 591, 747, 1401, 1407
MunI CAATTG 2 cut(s) 1353, 1548
Mva1269I GAATGC 2 cut(s) 619, 1364
MvaI CCWGG 4 cut(s) 319, 747, 1401, 1407
MvnI CGCG 2 cut(s) 399, 1555
MwoI GCNNNNNNNGC 8 cut(s) 296, 341, 920, 1130, 1133, 1139, 1379, 1734
NciI CCSGG 2 cut(s) 201, 591
NdeII GATC 2 cut(s) 228, 819
NlaIV GGNNCC 8 cut(s) 1055, 1168, 1198, 1441, 1485, 1530, 1596, 1723
NmuCI GTSAC 3 cut(s) 113, 187, 1246
NsiI ATGCAT 1 cut(s) 619
NspI RCATGY 1 cut(s) 351
NspV TTCGAA 2 cut(s) 406, 1777
PaqCI CACCTGC 1 cut(s) 719
PciI ACATGT 1 cut(s) 347
PciSI GCTCTTC 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 853
PctI GAATGC 2 cut(s) 619, 1364
PdmI GAANNNNTTC 2 cut(s) 363, 1287
PfeI GAWTC 6 cut(s) 491, 648, 783, 1228, 1392, 1499
PflFI GACNNNGTC 1 cut(s) 1580
PflMI CCANNNNNTGG 2 cut(s) 421, 1718
PfoI TCCNGGA 2 cut(s) 317, 589
PpuMI RGGWCCY 1 cut(s) 1439
PscI ACATGT 1 cut(s) 347
Psp124BI GAGCTC 3 cut(s) 239, 292, 337
Psp1406I AACGTT 1 cut(s) 546
Psp5II RGGWCCY 1 cut(s) 1439
Psp6I CCWGG 4 cut(s) 317, 745, 1399, 1405
PspFI CCCAGC 2 cut(s) 50, 1084
PspGI CCWGG 4 cut(s) 317, 745, 1399, 1405
PspN4I GGNNCC 8 cut(s) 1055, 1168, 1198, 1441, 1485, 1530, 1596, 1723
PspPI GGNCC 3 cut(s) 662, 1403, 1439
PspPPI RGGWCCY 1 cut(s) 1439
PstI CTGCAG 2 cut(s) 919, 1730
PstNI CAGNNNCTG 2 cut(s) 117, 1734
PsuI RGATCY 1 cut(s) 228
PsyI GACNNNGTC 1 cut(s) 1580
PvuII CAGCTG 1 cut(s) 920
RsaI GTAC 4 cut(s) 530, 577, 744, 956
RsaNI GTAC 4 cut(s) 529, 576, 743, 955
RseI CAYNNNNRTG 1 cut(s) 1214
SacI GAGCTC 3 cut(s) 239, 292, 337
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 4 cut(s) 278, 312, 1386, 1810
Sau3AI GATC 2 cut(s) 228, 819
Sau96I GGNCC 3 cut(s) 662, 1403, 1439
ScrFI CCNGG 6 cut(s) 201, 319, 591, 747, 1401, 1407
SduI GDGCHC 8 cut(s) 239, 292, 301, 337, 773, 1341, 1531, 1570
SfcI CTRYAG 3 cut(s) 764, 915, 1726
SfuI TTCGAA 2 cut(s) 406, 1777
SinI GGWCC 1 cut(s) 1439
SmiMI CAYNNNNRTG 1 cut(s) 1214
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
SsiI CCGC 6 cut(s) 397, 794, 1030, 1105, 1700, 1732
SspMI CTAG 3 cut(s) 222, 969, 1745
SstI GAGCTC 3 cut(s) 239, 292, 337
StyD4I CCNGG 6 cut(s) 199, 317, 589, 745, 1399, 1405
TaaI ACNGT 5 cut(s) 82, 187, 742, 1171, 1582
TaiI ACGT 3 cut(s) 549, 850, 1659
TaqI TCGA 5 cut(s) 406, 1145, 1659, 1777, 1801
TatI WGTACW 2 cut(s) 575, 954
TauI GCSGC 1 cut(s) 1734
TfiI GAWTC 6 cut(s) 491, 648, 783, 1228, 1392, 1499
Tru1I TTAA 4 cut(s) 278, 312, 1386, 1810
Tru9I TTAA 4 cut(s) 278, 312, 1386, 1810
TscAI CASTG 3 cut(s) 118, 1015, 1585
TseFI GTSAC 3 cut(s) 113, 187, 1246
Tsp45I GTSAC 3 cut(s) 113, 187, 1246
TspGWI ACGGA 1 cut(s) 671
TspRI CASTG 3 cut(s) 118, 1015, 1585
Tth111I GACNNNGTC 1 cut(s) 1580
Van91I CCANNNNNTGG 2 cut(s) 421, 1718
VpaK11BI GGWCC 1 cut(s) 1439
XagI CCTNNNNNAGG 1 cut(s) 1404
XapI RAATTY 3 cut(s) 363, 1175, 1287
XceI RCATGY 1 cut(s) 351
XcmI CCANNNNNNNNNTGG 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 1796
XmnI GAANNNNTTC 2 cut(s) 363, 1287
XspI CTAG 3 cut(s) 222, 969, 1745
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.