Rh4DG073300

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004128
Physical Location & Seq
Reverse (-)
10853991 .. 10860398
6408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG073300.1

Sequence Viewer

Length: 1554 bp
ATGGTGGGAAATAAAGTTAGCTTTTTGAGGCAGAGAGCTCAGGGTGCTCAGATTAGTTATAGATCGAGGAAGCACGCGGGAGCTCTCCATGCTACGTGTCTTGCTGATAAAATCCTGGTGGCAAACAGAGGAGAAATTGCTGTTCGTGTTATTCGAACGGCGCATGAGATGGGCATACCCTGTGTGGCTGTTTACTCAACAATAGACAAGGATGCACTTCATGTCAAATTGGCTGATGAATCAGTTTGTATTGGTGAGGCAGCAAGCAGTCAATCGTACCTAGTGGTTCCAAATGTTTTATCTGCTGCCATCAGTCGTAAATGTACAATGCTTCATCCTGGATATGGTTTCCTGTCTGAGAATGCATCTTTTGTTGAAATGTGCAAAGAACATGGAATCAACTTTATTGGTCCTAAGCCCGAAAGTATCCGTGTTATGGGTGATAAAGCGACTGCCAGAGAAACAATGAAGCAAGCAGGTGTTCCAACTGTACCAGGAAGTGATGGATTACTACAGAGCACAGAGGAAGGAATCAAGCTTGCCAATGAGATTGGTTATCCTGTTATGATCAAGGCAACAGCAGGTGGTGGAGGACGTGGTATGCGTCTTGCTAAAGAGCCTGAAGAGTTTGTAAAGTTGCTACAGGCAGCAAAGAGCGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTATGTCGTAAATCCTAGACACATTGAATTTCAGGTTCTTGCAGATAAGTACGGTAATGTTGTTCACTTTGGAGAGCGTGATTGTAGCATCCAGAGAAGGAACCAAAAGCTGTTAGAAGAAGCACCCTCTCCTGCATTGACCCCAGAATTGCGAAAAGCCATGGGTGATGCAGCAATTGCTGCTGCAGCATCTATTGGTTATATTGGTGTTGGAACCATCGAGTTCCTTTTGGACGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTAGGATTCAGGTTGAACATCCAGTGACAGAAATGATTTCCTCTGTTGACTTGATTGAAGAACAAATTCGTGTTGCTATGGGAGAAAAACTCCGATACACACAGGATGATATCGTGCTCAGAGGGCATTCAATTGAATGTCGTATCAATGCAGAAGATGCTTTTAAAGGATTCCGACCTGGGCCAGGTAGAATAACAGCTTACTTACCCGCCGGAGGTCCATTTGTTAGAATGGATAGTCATGTTTATCCTGATTATGTGGTTCCTCCAAACTATGATTCCCTTCTTGGAAAGCTTATTGTTTGGGCACCAACAAGAGAAAAGGCAATTGATCGGATGAAAAGAGCTCTTAACGACACTATTATAACAGGGGTTCCTACAACTATTGAATATCATAAGCTCATCCTGGAGATAGAGGATTTCAAAAATGGGAAGGTTGATACTGCTTTTATTCCCAAGCATGAAGAGGAGCTAGCAGCGGTAAGAAGCTATTTTAACCTTGAACATGACTTTACCAGTGACTTTGATTTTTTATTCTGGGTATTATGGTGGTCACAACTGTTTTCTTGGTGCAACAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

517

Amino Acids

57.34

Weight (kDa)

6.2

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 36 - 143 1e-41 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 148 - 355 4.4e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 157 - 325 4.5e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 175 - 324 3.6e-07 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 369 - 475 2.5e-38 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1334
AarI CACCTGC 2 cut(s) 467, 572
Acc36I ACCTGC 2 cut(s) 467, 572
AccB1I GGYRCC 1 cut(s) 1276
AccII CGCG 1 cut(s) 77
AciI CCGC 3 cut(s) 77, 1179, 1448
AcsI RAATTY 2 cut(s) 728, 1035
AcuI CTGAAG 1 cut(s) 642
AfaI GTAC 4 cut(s) 278, 325, 492, 752
AfiI CCNNNNNNNGG 4 cut(s) 344, 436, 1154, 1184
AflIII ACRYGT 1 cut(s) 95
AgsI TTSAA 9 cut(s) 377, 728, 986, 1028, 1101, 1106, 1358, 1393, 1472
AjiI CACGTC 1 cut(s) 596
AjnI CCWGG 6 cut(s) 114, 337, 493, 1147, 1153, 1374
AjuI GAANNNNNNNTTGG 2 cut(s) 1231, 1263
Alw21I GWGCWC 6 cut(s) 40, 49, 85, 521, 1089, 1318
AoxI GGCC 1 cut(s) 1151
ApoI RAATTY 2 cut(s) 728, 1035
Asp700I GAANNNNTTC 1 cut(s) 1035
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 3 cut(s) 410, 1151, 1187
AsuHPI GGTGA 3 cut(s) 266, 452, 878
AsuII TTCGAA 1 cut(s) 154
AsuNHI GCTAGC 1 cut(s) 1441
AvaII GGWCC 2 cut(s) 410, 1187
BaeGI GKGCMC 1 cut(s) 1279
BanI GGYRCC 1 cut(s) 1276
BanII GRGCYC 3 cut(s) 40, 85, 1318
Bbv12I GWGCWC 6 cut(s) 40, 49, 85, 521, 1089, 1318
BccI CCATC 5 cut(s) 163, 317, 497, 677, 926
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 6 cut(s) 116, 339, 495, 1149, 1155, 1376
BciVI GTATCC 1 cut(s) 437
BclI TGATCA 1 cut(s) 567
BfaI CTAG 4 cut(s) 281, 717, 972, 1442
BfmI CTRYAG 4 cut(s) 512, 641, 663, 885
BfuAI ACCTGC 2 cut(s) 467, 572
BfuI GTATCC 1 cut(s) 437
Bme1390I CCNGG 6 cut(s) 116, 339, 495, 1149, 1155, 1376
Bme18I GGWCC 2 cut(s) 410, 1187
BmgBI CACGTC 1 cut(s) 596
BmgT120I GGNCC 3 cut(s) 410, 1151, 1187
BmiI GGNNCC 7 cut(s) 288, 803, 916, 946, 1233, 1278, 1344
BmrFI CCNGG 6 cut(s) 116, 339, 495, 1149, 1155, 1376
BmsI GCATC 6 cut(s) 202, 374, 798, 859, 899, 1117
BmtI GCTAGC 1 cut(s) 1445
BplI GAGNNNNNCTC 2 cut(s) 1044, 1076
BpmI CTGGAG 1 cut(s) 1397
Bpu10I CCTNAGC 2 cut(s) 39, 414
Bpu14I TTCGAA 1 cut(s) 154
BsaAI YACGTR 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 861, 1148
Bsc4I CCNNNNNNNGG 4 cut(s) 344, 436, 1154, 1184
Bse1I ACTGG 2 cut(s) 992, 1485
BseBI CCWGG 6 cut(s) 116, 339, 495, 1149, 1155, 1376
BseDI CCNNGG 2 cut(s) 861, 1148
BseGI GGATG 7 cut(s) 217, 334, 789, 988, 1081, 1311, 1371
BseLI CCNNNNNNNGG 4 cut(s) 344, 436, 1154, 1184
BseMII CTCAG 4 cut(s) 53, 62, 348, 1102
BseNI ACTGG 2 cut(s) 992, 1485
BseRI GAGGAG 2 cut(s) 144, 1451
BseSI GKGCMC 1 cut(s) 1279
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 1 cut(s) 1153
BshNI GGYRCC 1 cut(s) 1276
BsiHKAI GWGCWC 6 cut(s) 40, 49, 85, 521, 1089, 1318
BsiSI CCGG 1 cut(s) 1182
BslI CCNNNNNNNGG 4 cut(s) 344, 436, 1154, 1184
BsmI GAATGC 2 cut(s) 367, 1096
BsnI GGCC 1 cut(s) 1153
Bsp119I TTCGAA 1 cut(s) 154
Bsp1286I GDGCHC 7 cut(s) 40, 49, 85, 521, 1089, 1279, 1318
Bsp1407I TGTACA 1 cut(s) 323
Bsp143I GATC 3 cut(s) 62, 567, 1300
Bsp19I CCATGG 1 cut(s) 861
BspACI CCGC 3 cut(s) 77, 1179, 1448
BspANI GGCC 1 cut(s) 1153
BspCNI CTCAG 4 cut(s) 52, 61, 349, 1101
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 7 cut(s) 288, 803, 916, 946, 1233, 1278, 1344
BspMAI CTGCAG 2 cut(s) 667, 889
BspMI ACCTGC 2 cut(s) 467, 572
BspOI GCTAGC 1 cut(s) 1445
BspT104I TTCGAA 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 1276
BsrGI TGTACA 1 cut(s) 323
BsrI ACTGG 2 cut(s) 992, 1485
BssECI CCNNGG 2 cut(s) 861, 1148
BssMI GATC 3 cut(s) 62, 567, 1300
BssT1I CCWWGG 1 cut(s) 861
Bst2UI CCWGG 6 cut(s) 116, 339, 495, 1149, 1155, 1376
Bst4CI ACNGT 3 cut(s) 490, 755, 1530
Bst6I CTCTTC 2 cut(s) 618, 1428
BstAPI GCANNNNNTGC 3 cut(s) 878, 881, 1127
BstAUI TGTACA 1 cut(s) 323
BstBAI YACGTR 1 cut(s) 96
BstBI TTCGAA 1 cut(s) 154
BstC8I GCNNGC 5 cut(s) 75, 265, 474, 540, 1443
BstDEI CTNAG 5 cut(s) 39, 48, 357, 414, 1088
BstDSI CCRYGG 1 cut(s) 861
BstENI CCTNNNNNAGG 1 cut(s) 1152
BstF5I GGATG 7 cut(s) 217, 334, 789, 988, 1081, 1311, 1371
BstFNI CGCG 1 cut(s) 77
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 3 cut(s) 65, 570, 1303
BstMBI GATC 3 cut(s) 62, 567, 1300
BstMWI GCNNNNNNNGC 8 cut(s) 44, 89, 668, 878, 881, 887, 1093, 1127
BstNI CCWGG 6 cut(s) 116, 339, 495, 1149, 1155, 1376
BstSCI CCNGG 6 cut(s) 114, 337, 493, 1147, 1153, 1374
BstSFI CTRYAG 4 cut(s) 512, 641, 663, 885
BstSLI GKGCMC 1 cut(s) 1279
BstUI CGCG 1 cut(s) 77
BsuI GTATCC 1 cut(s) 437
BsuRI GGCC 1 cut(s) 1153
BtgI CCRYGG 1 cut(s) 861
BtrI CACGTC 1 cut(s) 596
BtsCI GGATG 7 cut(s) 217, 334, 789, 988, 1081, 1311, 1371
BtsIMutI CAGTG 2 cut(s) 999, 1492
BveI ACCTGC 2 cut(s) 467, 572
Cac8I GCNNGC 5 cut(s) 75, 265, 474, 540, 1443
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 3 cut(s) 410, 1151, 1187
CseI GACGC 1 cut(s) 593
Csp6I GTAC 4 cut(s) 277, 324, 491, 751
CviAII CATG 9 cut(s) 89, 164, 221, 392, 862, 958, 1211, 1430, 1475
CviQI GTAC 4 cut(s) 277, 324, 491, 751
DdeI CTNAG 5 cut(s) 39, 48, 357, 414, 1088
DpnI GATC 3 cut(s) 64, 569, 1302
DpnII GATC 3 cut(s) 62, 567, 1300
DraI TTTAAA 1 cut(s) 1135
Eam1104I CTCTTC 2 cut(s) 618, 1428
EarI CTCTTC 2 cut(s) 618, 1428
Ecl136II GAGCTC 3 cut(s) 38, 83, 1316
Eco130I CCWWGG 1 cut(s) 861
Eco24I GRGCYC 3 cut(s) 40, 85, 1318
Eco32I GATATC 1 cut(s) 1081
Eco47I GGWCC 2 cut(s) 410, 1187
Eco53kI GAGCTC 3 cut(s) 38, 83, 1316
Eco57I CTGAAG 1 cut(s) 642
EcoICRI GAGCTC 3 cut(s) 38, 83, 1316
EcoNI CCTNNNNNAGG 1 cut(s) 1152
EcoRII CCWGG 6 cut(s) 114, 337, 493, 1147, 1153, 1374
EcoRV GATATC 1 cut(s) 1081
EcoT14I CCWWGG 1 cut(s) 861
EcoT22I ATGCAT 1 cut(s) 367
EcoT38I GRGCYC 3 cut(s) 40, 85, 1318
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 9 cut(s) 92, 167, 224, 395, 865, 961, 1214, 1433, 1478
FatI CATG 9 cut(s) 88, 163, 220, 391, 861, 957, 1210, 1429, 1474
FauI CCCGC 2 cut(s) 70, 1186
FbaI TGATCA 1 cut(s) 567
FokI GGATG 7 cut(s) 224, 321, 776, 975, 1088, 1318, 1358
FriOI GRGCYC 3 cut(s) 40, 85, 1318
FspBI CTAG 4 cut(s) 281, 717, 972, 1442
GlaI GCGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 1397
HaeIII GGCC 1 cut(s) 1153
HapII CCGG 1 cut(s) 1182
HgaI GACGC 1 cut(s) 593
HhaI GCGC 1 cut(s) 163
Hin1II CATG 9 cut(s) 92, 167, 224, 395, 865, 961, 1214, 1433, 1478
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HincII GTYRAC 1 cut(s) 1018
HindII GTYRAC 1 cut(s) 1018
HindIII AAGCTT 2 cut(s) 536, 1262
HinfI GANTC 6 cut(s) 239, 396, 531, 976, 1140, 1247
HpaII CCGG 1 cut(s) 1182
HphI GGTGA 3 cut(s) 266, 452, 878
Hpy166II GTNNAC 3 cut(s) 193, 766, 1018
Hpy188I TCNGA 6 cut(s) 51, 358, 1064, 1091, 1145, 1305
Hpy188III TCNNGA 2 cut(s) 793, 1220
Hpy8I GTNNAC 3 cut(s) 193, 766, 1018
HpyAV CCTTC 4 cut(s) 521, 792, 1262, 1396
HpyCH4III ACNGT 3 cut(s) 490, 755, 1530
HpyCH4IV ACGT 2 cut(s) 95, 595
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 89, 668, 878, 881, 887, 1093, 1127
HpyF3I CTNAG 5 cut(s) 39, 48, 357, 414, 1088
HpySE526I ACGT 2 cut(s) 95, 595
Hsp92II CATG 9 cut(s) 92, 167, 224, 395, 865, 961, 1214, 1433, 1478
HspAI GCGC 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 3 cut(s) 62, 567, 1300
LmnI GCTCC 2 cut(s) 80, 1438
LweI GCATC 6 cut(s) 202, 374, 798, 859, 899, 1117
MaeI CTAG 4 cut(s) 281, 717, 972, 1442
MaeII ACGT 2 cut(s) 95, 595
MaeIII GTNAC 3 cut(s) 994, 1487, 1521
MalI GATC 3 cut(s) 64, 569, 1302
MboI GATC 3 cut(s) 62, 567, 1300
MboII GAAGA 5 cut(s) 635, 830, 1040, 1136, 1445
MfeI CAATTG 3 cut(s) 876, 1101, 1296
MhlI GDGCHC 7 cut(s) 40, 49, 85, 521, 1089, 1279, 1318
MluCI AATT 8 cut(s) 135, 227, 728, 848, 876, 1035, 1101, 1296
MmeI TCCRAC 3 cut(s) 509, 892, 1168
Mph1103I ATGCAT 1 cut(s) 367
MroXI GAANNNNTTC 1 cut(s) 1035
MseI TTAA 4 cut(s) 1134, 1320, 1464, 1552
MslI CAYNNNNRTG 1 cut(s) 962
MspA1I CMGCKG 2 cut(s) 668, 1448
MspI CCGG 1 cut(s) 1182
MspR9I CCNGG 6 cut(s) 116, 339, 495, 1149, 1155, 1376
MunI CAATTG 3 cut(s) 876, 1101, 1296
Mva1269I GAATGC 2 cut(s) 367, 1096
MvaI CCWGG 6 cut(s) 116, 339, 495, 1149, 1155, 1376
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 8 cut(s) 44, 89, 668, 878, 881, 887, 1093, 1127
NcoI CCATGG 1 cut(s) 861
NdeII GATC 3 cut(s) 62, 567, 1300
NheI GCTAGC 1 cut(s) 1441
NlaIII CATG 9 cut(s) 92, 167, 224, 395, 865, 961, 1214, 1433, 1478
NlaIV GGNNCC 7 cut(s) 288, 803, 916, 946, 1233, 1278, 1344
NmuCI GTSAC 3 cut(s) 994, 1487, 1521
NsiI ATGCAT 1 cut(s) 367
NspV TTCGAA 1 cut(s) 154
PaqCI CACCTGC 2 cut(s) 467, 572
PcsI WCGNNNNNNNCGW 2 cut(s) 151, 601
PctI GAATGC 2 cut(s) 367, 1096
PdmI GAANNNNTTC 1 cut(s) 1035
PfeI GAWTC 6 cut(s) 239, 396, 531, 976, 1140, 1247
PfoI TCCNGGA 2 cut(s) 337, 1374
Ppu21I YACGTR 1 cut(s) 96
PsiI TTATAA 1 cut(s) 1334
Psp124BI GAGCTC 3 cut(s) 40, 85, 1318
Psp6I CCWGG 6 cut(s) 114, 337, 493, 1147, 1153, 1374
PspGI CCWGG 6 cut(s) 114, 337, 493, 1147, 1153, 1374
PspN4I GGNNCC 7 cut(s) 288, 803, 916, 946, 1233, 1278, 1344
PspPI GGNCC 3 cut(s) 410, 1151, 1187
PstI CTGCAG 2 cut(s) 667, 889
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 4 cut(s) 278, 325, 492, 752
RsaNI GTAC 4 cut(s) 277, 324, 491, 751
RseI CAYNNNNRTG 1 cut(s) 962
SacI GAGCTC 3 cut(s) 40, 85, 1318
SaqAI TTAA 4 cut(s) 1134, 1320, 1464, 1552
Sau3AI GATC 3 cut(s) 62, 567, 1300
Sau96I GGNCC 3 cut(s) 410, 1151, 1187
ScrFI CCNGG 6 cut(s) 116, 339, 495, 1149, 1155, 1376
SduI GDGCHC 7 cut(s) 40, 49, 85, 521, 1089, 1279, 1318
SfaNI GCATC 6 cut(s) 202, 374, 798, 859, 899, 1117
SfcI CTRYAG 4 cut(s) 512, 641, 663, 885
SfuI TTCGAA 1 cut(s) 154
SinI GGWCC 2 cut(s) 410, 1187
SmiMI CAYNNNNRTG 1 cut(s) 962
Sse9I AATT 8 cut(s) 135, 227, 728, 848, 876, 1035, 1101, 1296
SsiI CCGC 3 cut(s) 77, 1179, 1448
SspMI CTAG 4 cut(s) 281, 717, 972, 1442
SstI GAGCTC 3 cut(s) 40, 85, 1318
StyD4I CCNGG 6 cut(s) 114, 337, 493, 1147, 1153, 1374
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 3 cut(s) 490, 755, 1530
TaiI ACGT 2 cut(s) 98, 598
TaqI TCGA 3 cut(s) 65, 154, 921
TasI AATT 8 cut(s) 135, 227, 728, 848, 876, 1035, 1101, 1296
TatI WGTACW 1 cut(s) 323
TfiI GAWTC 6 cut(s) 239, 396, 531, 976, 1140, 1247
Tru1I TTAA 4 cut(s) 1134, 1320, 1464, 1552
Tru9I TTAA 4 cut(s) 1134, 1320, 1464, 1552
TscAI CASTG 2 cut(s) 999, 1492
TseFI GTSAC 3 cut(s) 994, 1487, 1521
Tsp45I GTSAC 3 cut(s) 994, 1487, 1521
TspDTI ATGAA 8 cut(s) 209, 252, 323, 482, 946, 980, 1322, 1446
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 2 cut(s) 999, 1492
VpaK11BI GGWCC 2 cut(s) 410, 1187
XagI CCTNNNNNAGG 1 cut(s) 1152
XapI RAATTY 2 cut(s) 728, 1035
XmnI GAANNNNTTC 1 cut(s) 1035
XspI CTAG 4 cut(s) 281, 717, 972, 1442
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.