MD15G1330100.v1.1

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004128
Physical Location & Seq
Reverse (-)
36348360 .. 36354713
6354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1330100.v1.1.491

Sequence Viewer

Length: 1587 bp
ATGCCCATTTGCAAGACTGTCACTCCGACTCCCGGCTTATTTTTTGGGAGGACAAGAGAGATCCGGAGTTCCCAATGTAGCTTTATGGTAGGAAATAAAGTCAGCTTTCTAAGGCAGAGGGCTCAGGGGGCTCAAGTTAGTTTTAAATCCAGGAAGCATGGGGGAGCTCTCCATGCTACATGTCGTGCTGAAAAAATTCTGGTGGCAAACAGAGGAGAAATTGCTGTTCGCGTTATTAGAACTGCCCATGAAATGGGAATACCTTGTGTTGCAGTTTACTCAACAATAGACAAGGATGCACTTCATGTGAAATTGGCCAATGAATCAGTTTGTATTGGTGAAGCTGCAAGCAGTCAGTCGTACTTAGTTGTTCCAAATGTTTTATCTGCTGCCATCAGTCGGAAATGTACAATGCTTCATCCAGGATATGGTTTCCTTTCTGAGAATGCATCCTTTGTTGAAATGTGCAGAGAACATGGAATCAACTTTATTGGGCCTAAGCCTGATAGCATCCGTGTTATGGGTGATAAATCGACTGCCAGGGACACAATGAAGAAAGCAGGCGTTCCAACTGTACCAGGAAGTGATGGATTACTACAGAGCACAGAAGAAGGAATCAGACTTGCAGATGAAATTGGTTTTCCTGTTATGATCAAGGCAACAGCAGGAGGTGGAGGACGTGGCATGCGTCTTGCTAAAGAACCTGAGGAGTTTGTGAAGCTGTTACAGCAAGCAAAAAGTGAGGCCGCAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTACATCCAAAATCCTAGACATATTGAATTTCAGGTTCTAGCAGATAAATATGGAAATGTTGTTCACTTTGGAGAGCGGGATTGCAGCATCCAGAGAAGGAACCAAAAGCTGCTGGAAGAAGCACCTTCCCCTGCATTGACCCCGGAATTGCGGAAAGCCATGGGTGATGCAGCAGTTGCTGCAGCTGCCTCGATTGGTTACGTTGGTGTTGGAACCGTTGAATTCCTTTTGGATGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTCGGATTCAGGTTGAACATCCTGTGACAGAAATGATTTCATCTGTTGACTTGATTGAAGAACAAATTCGTGTAGCTATGGGAGAAAAGCTCCGATACACACAGGATGAGATTGTGCTCAGAGGACATTCAATTGAATGTCGTATCAATGCAGAAGATGCTTTTAAAGGATTCCGACCTGGGCCAGGAAGAATAACAGCCTACTTACCATCCGGAGGTCCCTTTGTTAGAATGGATAGCCATGTTTATCCAGATTATGTGGTTCCTCCAAACTATGATTCCCTTCTTGGAAAGCTGATTGTGTGGGCACCAACAAGAGAAGGGGCAATTGAACGAATGAAGAGGGCTCTTGATGACACTGTTATAACAGGGGTTCCCACAACCATTGAATATCATAAACTTATCCTTGATATAGAGGATTTCAAAAATGGAAAAGTTGATACTGCTTTTATTCCAAAGCACGAGGAGGAGCTACGAGCGCCCCAACATTTGGTGCCCGCAGCTCCCAAAGAACTAGCTGGTGCATCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

529

Amino Acids

57.83

Weight (kDa)

7.22

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 64 - 171 6.7e-41 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 176 - 383 7.3e-82 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 198 - 353 5.3e-11 ATP-grasp domain
Dala_Dala_lig_C PF07478 207 - 352 9.6e-07 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 397 - 503 6.3e-38 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1418
AccB1I GGYRCC 2 cut(s) 1360, 1546
AccB7I CCANNNNNTGG 3 cut(s) 253, 428, 1543
AccBSI CCGCTC 1 cut(s) 862
AccII CGCG 1 cut(s) 231
AccIII TCCGGA 2 cut(s) 63, 1265
AciI CCGC 4 cut(s) 747, 862, 937, 1551
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 315
AcsI RAATTY 4 cut(s) 195, 812, 1007, 1119
AfaI GTAC 4 cut(s) 362, 409, 576, 788
AfiI CCNNNNNNNGG 8 cut(s) 32, 253, 399, 428, 520, 1238, 1268, 1543
AflIII ACRYGT 1 cut(s) 179
AjiI CACGTC 1 cut(s) 680
AjnI CCWGG 6 cut(s) 149, 421, 539, 577, 1231, 1237
AjuI GAANNNNNNNTTGG 2 cut(s) 1315, 1347
AloI GAACNNNNNNTCC 2 cut(s) 831, 863
Alw21I GWGCWC 3 cut(s) 169, 605, 1173
AlwI GGATC 1 cut(s) 55
AlwNI CAGNNNCTG 1 cut(s) 965
Aor13HI TCCGGA 2 cut(s) 63, 1265
AoxI GGCC 4 cut(s) 315, 494, 744, 1235
ApoI RAATTY 4 cut(s) 195, 812, 1007, 1119
Asp700I GAANNNNTTC 2 cut(s) 195, 1119
AspLEI GCGC 1 cut(s) 1534
AspS9I GGNCC 3 cut(s) 494, 1235, 1271
AsuC2I CCSGG 2 cut(s) 33, 929
AsuHPI GGTGA 3 cut(s) 350, 536, 962
AvaII GGWCC 1 cut(s) 1271
AxyI CCTNAGG 1 cut(s) 705
BaeGI GKGCMC 2 cut(s) 1363, 1551
BalI TGGCCA 1 cut(s) 317
BanI GGYRCC 2 cut(s) 1360, 1546
BanII GRGCYC 4 cut(s) 124, 133, 169, 1402
BauI CACGAG 1 cut(s) 1514
Bbv12I GWGCWC 3 cut(s) 169, 605, 1173
BccI CCATC 4 cut(s) 401, 581, 761, 1270
BcgI CGANNNNNNTGC 2 cut(s) 957, 991
BciT130I CCWGG 6 cut(s) 151, 423, 541, 579, 1233, 1239
BclI TGATCA 1 cut(s) 651
BcnI CCSGG 2 cut(s) 33, 929
BfaI CTAG 3 cut(s) 801, 824, 1568
BfmI CTRYAG 2 cut(s) 596, 966
BfoI RGCGCY 1 cut(s) 1535
Bme1390I CCNGG 8 cut(s) 33, 151, 423, 541, 579, 929, 1233, 1239
Bme18I GGWCC 1 cut(s) 1271
BmgBI CACGTC 1 cut(s) 680
BmgT120I GGNCC 3 cut(s) 494, 1235, 1271
BmiI GGNNCC 8 cut(s) 887, 1000, 1030, 1273, 1317, 1362, 1428, 1548
BmrFI CCNGG 8 cut(s) 33, 151, 423, 541, 579, 929, 1233, 1239
BmsI GCATC 6 cut(s) 286, 458, 519, 882, 943, 1201
BplI GAGNNNNNCTC 2 cut(s) 1128, 1160
Bpu10I CCTNAGC 2 cut(s) 123, 498
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 2 cut(s) 33, 929
BsaJI CCNNGG 4 cut(s) 540, 927, 945, 1232
BsaWI WCCGGW 2 cut(s) 63, 1265
BsaXI ACNNNNNCTCC 4 cut(s) 7, 13, 37, 43
Bsc4I CCNNNNNNNGG 8 cut(s) 32, 253, 399, 428, 520, 1238, 1268, 1543
Bse21I CCTNAGG 1 cut(s) 705
BseAI TCCGGA 2 cut(s) 63, 1265
BseBI CCWGG 6 cut(s) 151, 423, 541, 579, 1233, 1239
BseDI CCNNGG 4 cut(s) 540, 927, 945, 1232
BseLI CCNNNNNNNGG 8 cut(s) 32, 253, 399, 428, 520, 1238, 1268, 1543
BseMII CTCAG 4 cut(s) 137, 432, 696, 1186
BseRI GAGGAG 4 cut(s) 228, 722, 1532, 1535
BseSI GKGCMC 2 cut(s) 1363, 1551
BsgI GTGCAG 1 cut(s) 487
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 4 cut(s) 317, 496, 746, 1237
BshNI GGYRCC 2 cut(s) 1360, 1546
BsiHKAI GWGCWC 3 cut(s) 169, 605, 1173
BsiSI CCGG 4 cut(s) 33, 64, 929, 1266
BslFI GGGAC 2 cut(s) 557, 1257
BslI CCNNNNNNNGG 8 cut(s) 32, 253, 399, 428, 520, 1238, 1268, 1543
BsmFI GGGAC 2 cut(s) 557, 1257
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 4 cut(s) 317, 496, 746, 1237
Bsp1286I GDGCHC 8 cut(s) 124, 133, 169, 605, 1173, 1363, 1402, 1551
Bsp13I TCCGGA 2 cut(s) 63, 1265
Bsp1407I TGTACA 1 cut(s) 407
Bsp143I GATC 2 cut(s) 60, 651
Bsp19I CCATGG 1 cut(s) 945
BspACI CCGC 4 cut(s) 747, 862, 937, 1551
BspANI GGCC 4 cut(s) 317, 496, 746, 1237
BspCNI CTCAG 4 cut(s) 136, 433, 697, 1185
BspEI TCCGGA 2 cut(s) 63, 1265
BspFNI CGCG 1 cut(s) 231
BspLI GGNNCC 8 cut(s) 887, 1000, 1030, 1273, 1317, 1362, 1428, 1548
BspMAI CTGCAG 1 cut(s) 970
BspPI GGATC 1 cut(s) 55
BspT107I GGYRCC 2 cut(s) 1360, 1546
BsrBI CCGCTC 1 cut(s) 862
BsrGI TGTACA 1 cut(s) 407
BssECI CCNNGG 4 cut(s) 540, 927, 945, 1232
BssMI GATC 2 cut(s) 60, 651
BssSI CACGAG 1 cut(s) 1514
BssT1I CCWWGG 1 cut(s) 945
Bst2BI CACGAG 1 cut(s) 1514
Bst2UI CCWGG 6 cut(s) 151, 423, 541, 579, 1233, 1239
Bst4CI ACNGT 4 cut(s) 19, 574, 1003, 1414
Bst6I CTCTTC 1 cut(s) 1388
BstAPI GCANNNNNTGC 3 cut(s) 962, 965, 1211
BstAUI TGTACA 1 cut(s) 407
BstC8I GCNNGC 5 cut(s) 349, 562, 686, 732, 1551
BstDEI CTNAG 8 cut(s) 110, 123, 364, 441, 498, 705, 1172, 1584
BstDSI CCRYGG 1 cut(s) 945
BstENI CCTNNNNNAGG 1 cut(s) 1236
BstFNI CGCG 1 cut(s) 231
BstH2I RGCGCY 1 cut(s) 1535
BstHHI GCGC 1 cut(s) 1534
BstKTI GATC 2 cut(s) 63, 654
BstMBI GATC 2 cut(s) 60, 651
BstMWI GCNNNNNNNGC 9 cut(s) 128, 173, 727, 752, 962, 965, 971, 1211, 1531
BstNI CCWGG 6 cut(s) 151, 423, 541, 579, 1233, 1239
BstNSI RCATGY 2 cut(s) 183, 688
BstSCI CCNGG 8 cut(s) 31, 149, 421, 539, 577, 927, 1231, 1237
BstSFI CTRYAG 2 cut(s) 596, 966
BstSLI GKGCMC 2 cut(s) 1363, 1551
BstUI CGCG 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
Bsu36I CCTNAGG 1 cut(s) 705
BsuRI GGCC 4 cut(s) 317, 496, 746, 1237
BtgI CCRYGG 1 cut(s) 945
BtrI CACGTC 1 cut(s) 680
BtsIMutI CAGTG 1 cut(s) 1410
Cac8I GCNNGC 5 cut(s) 349, 562, 686, 732, 1551
CaiI CAGNNNCTG 1 cut(s) 965
CfoI GCGC 1 cut(s) 1534
Cfr13I GGNCC 3 cut(s) 494, 1235, 1271
CseI GACGC 1 cut(s) 677
Csp6I GTAC 4 cut(s) 361, 408, 575, 787
CspCI CAANNNNNGTGG 2 cut(s) 1420, 1455
CviQI GTAC 4 cut(s) 361, 408, 575, 787
DdeI CTNAG 8 cut(s) 110, 123, 364, 441, 498, 705, 1172, 1584
DpnI GATC 2 cut(s) 62, 653
DpnII GATC 2 cut(s) 60, 651
DraI TTTAAA 2 cut(s) 145, 1219
EaeI YGGCCR 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 1388
EarI CTCTTC 1 cut(s) 1388
Ecl136II GAGCTC 1 cut(s) 167
Eco130I CCWWGG 1 cut(s) 945
Eco24I GRGCYC 4 cut(s) 124, 133, 169, 1402
Eco47I GGWCC 1 cut(s) 1271
Eco53kI GAGCTC 1 cut(s) 167
Eco81I CCTNAGG 1 cut(s) 705
EcoICRI GAGCTC 1 cut(s) 167
EcoNI CCTNNNNNAGG 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 1271
EcoRI GAATTC 1 cut(s) 1007
EcoRII CCWGG 6 cut(s) 149, 421, 539, 577, 1231, 1237
EcoT14I CCWWGG 1 cut(s) 945
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 4 cut(s) 124, 133, 169, 1402
ErhI CCWWGG 1 cut(s) 945
FaqI GGGAC 2 cut(s) 557, 1257
FauI CCCGC 2 cut(s) 855, 1558
FbaI TGATCA 1 cut(s) 651
FriOI GRGCYC 4 cut(s) 124, 133, 169, 1402
FspBI CTAG 3 cut(s) 801, 824, 1568
GlaI GCGC 1 cut(s) 1533
HaeII RGCGCY 1 cut(s) 1535
HaeIII GGCC 4 cut(s) 317, 496, 746, 1237
HapII CCGG 4 cut(s) 33, 64, 929, 1266
HgaI GACGC 1 cut(s) 677
HhaI GCGC 1 cut(s) 1534
Hin6I GCGC 1 cut(s) 1532
HinP1I GCGC 1 cut(s) 1532
HincII GTYRAC 1 cut(s) 1102
HindII GTYRAC 1 cut(s) 1102
HinfI GANTC 7 cut(s) 28, 323, 480, 615, 1060, 1224, 1331
HpaII CCGG 4 cut(s) 33, 64, 929, 1266
HphI GGTGA 3 cut(s) 350, 536, 962
Hpy166II GTNNAC 3 cut(s) 277, 850, 1102
Hpy188I TCNGA 8 cut(s) 27, 402, 442, 620, 1059, 1148, 1175, 1229
Hpy188III TCNNGA 5 cut(s) 64, 877, 1266, 1304, 1403
Hpy8I GTNNAC 3 cut(s) 277, 850, 1102
HpyAV CCTTC 5 cut(s) 605, 876, 921, 1346, 1367
HpyCH4III ACNGT 4 cut(s) 19, 574, 1003, 1414
HpyCH4IV ACGT 2 cut(s) 679, 987
HpyF10VI GCNNNNNNNGC 9 cut(s) 128, 173, 727, 752, 962, 965, 971, 1211, 1531
HpyF3I CTNAG 8 cut(s) 110, 123, 364, 441, 498, 705, 1172, 1584
HpySE526I ACGT 2 cut(s) 679, 987
HspAI GCGC 1 cut(s) 1532
Kpn2I TCCGGA 2 cut(s) 63, 1265
Ksp22I TGATCA 1 cut(s) 651
Kzo9I GATC 2 cut(s) 60, 651
LmnI GCTCC 4 cut(s) 164, 1149, 1522, 1561
LweI GCATC 6 cut(s) 286, 458, 519, 882, 943, 1201
MaeI CTAG 3 cut(s) 801, 824, 1568
MaeII ACGT 2 cut(s) 679, 987
MaeIII GTNAC 4 cut(s) 19, 723, 983, 1078
MalI GATC 2 cut(s) 62, 653
MbiI CCGCTC 1 cut(s) 862
MboI GATC 2 cut(s) 60, 651
MboII GAAGA 7 cut(s) 565, 620, 914, 1124, 1220, 1254, 1405
MfeI CAATTG 2 cut(s) 1185, 1380
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 8 cut(s) 124, 133, 169, 605, 1173, 1363, 1402, 1551
MlsI TGGCCA 1 cut(s) 317
MluNI TGGCCA 1 cut(s) 317
MlyI GAGTC 1 cut(s) 22
MmeI TCCRAC 5 cut(s) 50, 380, 593, 976, 1252
Mox20I TGGCCA 1 cut(s) 317
Mph1103I ATGCAT 1 cut(s) 451
MroI TCCGGA 2 cut(s) 63, 1265
MroXI GAANNNNTTC 2 cut(s) 195, 1119
MscI TGGCCA 1 cut(s) 317
MseI TTAA 2 cut(s) 144, 1218
MslI CAYNNNNRTG 1 cut(s) 1046
Msp20I TGGCCA 1 cut(s) 317
MspA1I CMGCKG 2 cut(s) 752, 971
MspI CCGG 4 cut(s) 33, 64, 929, 1266
MspR9I CCNGG 8 cut(s) 33, 151, 423, 541, 579, 929, 1233, 1239
MunI CAATTG 2 cut(s) 1185, 1380
Mva1269I GAATGC 1 cut(s) 451
MvaI CCWGG 6 cut(s) 151, 423, 541, 579, 1233, 1239
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 9 cut(s) 128, 173, 727, 752, 962, 965, 971, 1211, 1531
NciI CCSGG 2 cut(s) 33, 929
NcoI CCATGG 1 cut(s) 945
NdeII GATC 2 cut(s) 60, 651
NlaIV GGNNCC 8 cut(s) 887, 1000, 1030, 1273, 1317, 1362, 1428, 1548
NmuCI GTSAC 2 cut(s) 19, 1078
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 2 cut(s) 183, 688
PaeI GCATGC 1 cut(s) 688
PciI ACATGT 1 cut(s) 179
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PctI GAATGC 1 cut(s) 451
PdmI GAANNNNTTC 2 cut(s) 195, 1119
PfeI GAWTC 6 cut(s) 323, 480, 615, 1060, 1224, 1331
PflMI CCANNNNNTGG 3 cut(s) 253, 428, 1543
PfoI TCCNGGA 2 cut(s) 149, 421
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
PpuMI RGGWCCY 1 cut(s) 1271
PscI ACATGT 1 cut(s) 179
PsiI TTATAA 1 cut(s) 1418
Psp124BI GAGCTC 1 cut(s) 169
Psp5II RGGWCCY 1 cut(s) 1271
Psp6I CCWGG 6 cut(s) 149, 421, 539, 577, 1231, 1237
PspGI CCWGG 6 cut(s) 149, 421, 539, 577, 1231, 1237
PspN4I GGNNCC 8 cut(s) 887, 1000, 1030, 1273, 1317, 1362, 1428, 1548
PspPI GGNCC 3 cut(s) 494, 1235, 1271
PspPPI RGGWCCY 1 cut(s) 1271
PstI CTGCAG 1 cut(s) 970
PstNI CAGNNNCTG 1 cut(s) 965
PsuI RGATCY 1 cut(s) 60
PvuII CAGCTG 2 cut(s) 752, 971
RsaI GTAC 4 cut(s) 362, 409, 576, 788
RsaNI GTAC 4 cut(s) 361, 408, 575, 787
RseI CAYNNNNRTG 1 cut(s) 1046
SacI GAGCTC 1 cut(s) 169
SaqAI TTAA 2 cut(s) 144, 1218
Sau3AI GATC 2 cut(s) 60, 651
Sau96I GGNCC 3 cut(s) 494, 1235, 1271
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 8 cut(s) 33, 151, 423, 541, 579, 929, 1233, 1239
SduI GDGCHC 8 cut(s) 124, 133, 169, 605, 1173, 1363, 1402, 1551
SfaNI GCATC 6 cut(s) 286, 458, 519, 882, 943, 1201
SfcI CTRYAG 2 cut(s) 596, 966
SinI GGWCC 1 cut(s) 1271
SmiMI CAYNNNNRTG 1 cut(s) 1046
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
SphI GCATGC 1 cut(s) 688
SsiI CCGC 4 cut(s) 747, 862, 937, 1551
SspMI CTAG 3 cut(s) 801, 824, 1568
SstI GAGCTC 1 cut(s) 169
StyD4I CCNGG 8 cut(s) 31, 149, 421, 539, 577, 927, 1231, 1237
StyI CCWWGG 1 cut(s) 945
TaaI ACNGT 4 cut(s) 19, 574, 1003, 1414
TaiI ACGT 2 cut(s) 682, 990
TaqI TCGA 2 cut(s) 533, 977
TatI WGTACW 2 cut(s) 407, 786
TauI GCSGC 1 cut(s) 749
TfiI GAWTC 6 cut(s) 323, 480, 615, 1060, 1224, 1331
Tru1I TTAA 2 cut(s) 144, 1218
Tru9I TTAA 2 cut(s) 144, 1218
TscAI CASTG 1 cut(s) 1417
TseFI GTSAC 2 cut(s) 19, 1078
Tsp45I GTSAC 2 cut(s) 19, 1078
TspGWI ACGGA 1 cut(s) 503
TspRI CASTG 1 cut(s) 1417
Van91I CCANNNNNTGG 3 cut(s) 253, 428, 1543
VpaK11BI GGWCC 1 cut(s) 1271
XagI CCTNNNNNAGG 1 cut(s) 1236
XapI RAATTY 4 cut(s) 195, 812, 1007, 1119
XceI RCATGY 2 cut(s) 183, 688
XmnI GAANNNNTTC 2 cut(s) 195, 1119
XspI CTAG 3 cut(s) 801, 824, 1568
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.