MD13G1233600.v1.1

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004128
Physical Location & Seq
Reverse (-)
23321030 .. 23332498
11469 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1233600.v1.1.491

Sequence Viewer

Length: 1572 bp
ATGCCCATCTGCAAGACTGTCACGCCAACTCCGGGCTTATTTTTTGGGAGAACTAGAGAGATCCGCAGTTCTCAATGTAGCTTTATGGTGGGAAATAAAGTCAACTTTTTAAGGCAGAGAGTTCAGGGTGCTCAAGTTAGTGTTAAATCCAGAAAGCATGGGGGAGCTCTTCATGCTACATGTCATGCTGAAAAAATTCTGGTGGCAAATAGAGGGGAAATTGCTGTTCGCGTTATTCGAACAGCCCATGAGATGGGAATACCTTGTGTGGCTGTTTACTCAACAATAGACAAGGATGCACTTCATGTGAAATTGGCTGACGAATCAGTTTGTATTGGTGAAGCAGCAAGCAGTCAATCGTACTTAGTTGTTCCAAATGTTTTATCTGCTGCCATCAGTCGGAAATGTACAATGTTGCATCCAGGATATGGTTTCCTTTCTGAGAATGCATCATTTGTTGAAATGTGCAAAGAACATGGAATCAATTTTATTGGGCCTAAGCCCGAGAGCATCCGTGTTATGGGTGATAAATCTACTGCCAGAGACACAATGAAGAAAGCAGGTGTCCCAACTGTACCTGGAAGTGATGGATTACTACAGAGCACAGAGGAAGGAATCAGGCTTGCAGATGAGATTGGTTTTCCTGTAATGATCAAGGCCACAGCAGGAGGTGGAGGACGTGGCATGCGTCTTGCTAAAGAACCTGAGGAGTTTGTGAAGTTGTTGCAGCAAGCAAAGAGTGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTACATCCAAAATCCTAGACACATTGAGTTTCAGGTTCTTGCAGATAAATATGGTAATGTTATTCACTTTGGAGAGCGGGATTGCAGCATCCAGAGAAGGAACCAAAAGCTTCTGGAAGAAGCACCTTCCCCTGCATTGACCCCAGAACTGCGGAAAGCTATGGGTGATGCAGCAGTTGCAGCAGCAGCATCAATTGGCTACATTGGTGTTGGAACCGTTGAATTCCTTTTGGACGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTCGGATTCAGGTTGAGCATCCTGTGACAGAAATGATTTCCTCTGTTGACTTGATTGAAGAACAAATTCATGTGGCTATGGGTGAAAAACTCCGATACACACAGGAAGATATAGTGCTCAGAGGACATTCAATTGAATGCCGTATCAATGCAGAAGATGCTTTTAAAGGATTCCGACCTGGGCCAGGAAGAATAACAGCCTACTTACCATCTGGAGGTCCCTTTGTTCGAATGGATAGCCATGTTTATCCTGATTATGTGGTTCCTCCAAACTATGATTCCCTTCTTGGAAAGCTTATTGTTTGGGCACCAACAAGAGAAAAGGCGATTGAGCGAATGAAGAGGGCTCTTGACGACACTGTTATAACGGGGGTTCCCACAACCATTGAATACCATAAACTTATCCTTGACATAGAGGATTTCAAAAATGGCAAGGTTGATACTGCTTTTATTCCAAAGCATGAAGAGGAGCTACAAGCGCCCCAACATTTGGTGCCCGCGGCCGCCGCCAAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

524

Amino Acids

57.42

Weight (kDa)

6.77

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 64 - 171 7.7e-42 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 176 - 383 3.3e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 198 - 353 1.6e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 207 - 352 1.3e-06 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 397 - 503 2e-38 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1418
AarI CACCTGC 1 cut(s) 551
Acc36I ACCTGC 1 cut(s) 551
AccB1I GGYRCC 2 cut(s) 1360, 1546
AccB7I CCANNNNNTGG 3 cut(s) 253, 428, 1543
AccBSI CCGCTC 1 cut(s) 862
AccII CGCG 2 cut(s) 231, 1553
AciI CCGC 7 cut(s) 64, 862, 937, 1551, 1553, 1557, 1560
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 1554
AcsI RAATTY 3 cut(s) 195, 1007, 1119
AfaI GTAC 4 cut(s) 362, 409, 576, 788
AfiI CCNNNNNNNGG 8 cut(s) 32, 253, 399, 428, 520, 1238, 1268, 1543
AflIII ACRYGT 1 cut(s) 179
AgsI TTSAA 7 cut(s) 461, 1007, 1112, 1185, 1190, 1442, 1477
AjiI CACGTC 1 cut(s) 680
AjnI CCWGG 4 cut(s) 421, 577, 1231, 1237
AjuI GAANNNNNNNTTGG 2 cut(s) 1315, 1347
AluBI AGCT 7 cut(s) 81, 167, 752, 895, 944, 1348, 1525
AluI AGCT 7 cut(s) 81, 167, 752, 895, 944, 1348, 1525
Alw21I GWGCWC 4 cut(s) 133, 169, 605, 1173
Alw26I GTCTC 1 cut(s) 537
AlwI GGATC 1 cut(s) 55
Ama87I CYCGRG 1 cut(s) 503
AoxI GGCC 4 cut(s) 494, 657, 1235, 1554
ApoI RAATTY 3 cut(s) 195, 1007, 1119
Asp700I GAANNNNTTC 2 cut(s) 195, 1119
AspLEI GCGC 1 cut(s) 1534
AspS9I GGNCC 3 cut(s) 494, 1235, 1271
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 4 cut(s) 350, 536, 962, 1148
AsuII TTCGAA 2 cut(s) 238, 1282
AvaI CYCGRG 1 cut(s) 503
AvaII GGWCC 1 cut(s) 1271
AxyI CCTNAGG 1 cut(s) 705
BaeGI GKGCMC 2 cut(s) 1363, 1551
BanI GGYRCC 2 cut(s) 1360, 1546
BanII GRGCYC 2 cut(s) 169, 1402
BarI GAAGNNNNNNTAC 2 cut(s) 1509, 1541
Bbv12I GWGCWC 4 cut(s) 133, 169, 605, 1173
BccI CCATC 6 cut(s) 14, 247, 401, 581, 761, 1270
BceAI ACGGC 1 cut(s) 1179
BciT130I CCWGG 4 cut(s) 423, 579, 1233, 1239
BclI TGATCA 1 cut(s) 651
BcnI CCSGG 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 3 cut(s) 54, 801, 1570
BfmI CTRYAG 2 cut(s) 596, 747
BfoI RGCGCY 1 cut(s) 1535
BfuAI ACCTGC 1 cut(s) 551
Bme1390I CCNGG 5 cut(s) 33, 423, 579, 1233, 1239
Bme18I GGWCC 1 cut(s) 1271
BmeT110I CYCGRG 1 cut(s) 503
BmgBI CACGTC 1 cut(s) 680
BmgT120I GGNCC 3 cut(s) 494, 1235, 1271
BmiI GGNNCC 8 cut(s) 887, 1000, 1030, 1273, 1317, 1362, 1428, 1548
BmrFI CCNGG 5 cut(s) 33, 423, 579, 1233, 1239
BmsI GCATC 9 cut(s) 286, 427, 458, 519, 882, 943, 983, 1081, 1201
BpmI CTGGAG 1 cut(s) 1287
Bpu10I CCTNAGC 1 cut(s) 498
Bpu14I TTCGAA 2 cut(s) 238, 1282
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 33
BsaJI CCNNGG 2 cut(s) 1232, 1551
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 8 cut(s) 32, 253, 399, 428, 520, 1238, 1268, 1543
Bse21I CCTNAGG 1 cut(s) 705
BseBI CCWGG 4 cut(s) 423, 579, 1233, 1239
BseDI CCNNGG 2 cut(s) 1232, 1551
BseGI GGATG 6 cut(s) 301, 418, 510, 789, 873, 1072
BseLI CCNNNNNNNGG 8 cut(s) 32, 253, 399, 428, 520, 1238, 1268, 1543
BseMII CTCAG 3 cut(s) 432, 696, 1186
BseRI GAGGAG 2 cut(s) 722, 1535
BseSI GKGCMC 2 cut(s) 1363, 1551
BseX3I CGGCCG 1 cut(s) 1554
Bsh1236I CGCG 2 cut(s) 231, 1553
Bsh1285I CGRYCG 1 cut(s) 1557
BshFI GGCC 4 cut(s) 496, 659, 1237, 1556
BshNI GGYRCC 2 cut(s) 1360, 1546
BsiEI CGRYCG 1 cut(s) 1557
BsiHKAI GWGCWC 4 cut(s) 133, 169, 605, 1173
BsiHKCI CYCGRG 1 cut(s) 503
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 2 cut(s) 551, 1257
BslI CCNNNNNNNGG 8 cut(s) 32, 253, 399, 428, 520, 1238, 1268, 1543
BsmAI GTCTC 1 cut(s) 537
BsmFI GGGAC 2 cut(s) 551, 1257
BsmI GAATGC 2 cut(s) 451, 1196
BsnI GGCC 4 cut(s) 496, 659, 1237, 1556
BsoBI CYCGRG 1 cut(s) 503
Bsp119I TTCGAA 2 cut(s) 238, 1282
Bsp1286I GDGCHC 7 cut(s) 133, 169, 605, 1173, 1363, 1402, 1551
Bsp1407I TGTACA 1 cut(s) 407
Bsp143I GATC 2 cut(s) 60, 651
BspACI CCGC 7 cut(s) 64, 862, 937, 1551, 1553, 1557, 1560
BspANI GGCC 4 cut(s) 496, 659, 1237, 1556
BspCNI CTCAG 3 cut(s) 433, 697, 1185
BspFNI CGCG 2 cut(s) 231, 1553
BspLI GGNNCC 8 cut(s) 887, 1000, 1030, 1273, 1317, 1362, 1428, 1548
BspMAI CTGCAG 1 cut(s) 751
BspMI ACCTGC 1 cut(s) 551
BspPI GGATC 1 cut(s) 55
BspQI GCTCTTC 1 cut(s) 174
BspT104I TTCGAA 2 cut(s) 238, 1282
BspT107I GGYRCC 2 cut(s) 1360, 1546
BsrBI CCGCTC 1 cut(s) 862
BsrGI TGTACA 1 cut(s) 407
BssECI CCNNGG 2 cut(s) 1232, 1551
BssMI GATC 2 cut(s) 60, 651
Bst2UI CCWGG 4 cut(s) 423, 579, 1233, 1239
Bst4CI ACNGT 4 cut(s) 19, 574, 1003, 1414
Bst6I CTCTTC 3 cut(s) 174, 1388, 1512
BstAPI GCANNNNNTGC 2 cut(s) 962, 1211
BstAUI TGTACA 1 cut(s) 407
BstBI TTCGAA 2 cut(s) 238, 1282
BstC8I GCNNGC 5 cut(s) 349, 624, 686, 732, 1551
BstDEI CTNAG 5 cut(s) 364, 441, 498, 705, 1172
BstDSI CCRYGG 1 cut(s) 1551
BstENI CCTNNNNNAGG 1 cut(s) 1236
BstF5I GGATG 6 cut(s) 301, 418, 510, 789, 873, 1072
BstFNI CGCG 2 cut(s) 231, 1553
BstH2I RGCGCY 1 cut(s) 1535
BstHHI GCGC 1 cut(s) 1534
BstKTI GATC 2 cut(s) 63, 654
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 2 cut(s) 60, 651
BstMCI CGRYCG 1 cut(s) 1557
BstMWI GCNNNNNNNGC 8 cut(s) 173, 752, 962, 965, 971, 1211, 1531, 1559
BstNI CCWGG 4 cut(s) 423, 579, 1233, 1239
BstNSI RCATGY 2 cut(s) 183, 688
BstSCI CCNGG 5 cut(s) 31, 421, 577, 1231, 1237
BstSFI CTRYAG 2 cut(s) 596, 747
BstSLI GKGCMC 2 cut(s) 1363, 1551
BstUI CGCG 2 cut(s) 231, 1553
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BstZI CGGCCG 1 cut(s) 1554
Bsu36I CCTNAGG 1 cut(s) 705
BsuRI GGCC 4 cut(s) 496, 659, 1237, 1556
BtgI CCRYGG 1 cut(s) 1551
BtrI CACGTC 1 cut(s) 680
BtsCI GGATG 6 cut(s) 301, 418, 510, 789, 873, 1072
BtsIMutI CAGTG 1 cut(s) 1410
BveI ACCTGC 1 cut(s) 551
Cac8I GCNNGC 5 cut(s) 349, 624, 686, 732, 1551
CciNI GCGGCCGC 1 cut(s) 1554
CfoI GCGC 1 cut(s) 1534
Cfr13I GGNCC 3 cut(s) 494, 1235, 1271
Cfr42I CCGCGG 1 cut(s) 1554
CseI GACGC 1 cut(s) 677
Csp6I GTAC 4 cut(s) 361, 408, 575, 787
CspCI CAANNNNNGTGG 2 cut(s) 1420, 1455
CviQI GTAC 4 cut(s) 361, 408, 575, 787
DdeI CTNAG 5 cut(s) 364, 441, 498, 705, 1172
DpnI GATC 2 cut(s) 62, 653
DpnII GATC 2 cut(s) 60, 651
DraI TTTAAA 1 cut(s) 1219
EaeI YGGCCR 1 cut(s) 1554
EagI CGGCCG 1 cut(s) 1554
Eam1104I CTCTTC 3 cut(s) 174, 1388, 1512
EarI CTCTTC 3 cut(s) 174, 1388, 1512
Ecl136II GAGCTC 1 cut(s) 167
EclXI CGGCCG 1 cut(s) 1554
Eco24I GRGCYC 2 cut(s) 169, 1402
Eco47I GGWCC 1 cut(s) 1271
Eco52I CGGCCG 1 cut(s) 1554
Eco53kI GAGCTC 1 cut(s) 167
Eco81I CCTNAGG 1 cut(s) 705
Eco88I CYCGRG 1 cut(s) 503
EcoICRI GAGCTC 1 cut(s) 167
EcoNI CCTNNNNNAGG 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 1271
EcoRI GAATTC 1 cut(s) 1007
EcoRII CCWGG 4 cut(s) 421, 577, 1231, 1237
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 2 cut(s) 169, 1402
FaqI GGGAC 2 cut(s) 551, 1257
FauI CCCGC 2 cut(s) 855, 1558
FbaI TGATCA 1 cut(s) 651
FokI GGATG 6 cut(s) 308, 405, 497, 776, 860, 1059
FriOI GRGCYC 2 cut(s) 169, 1402
FspBI CTAG 3 cut(s) 54, 801, 1570
GlaI GCGC 1 cut(s) 1533
GsuI CTGGAG 1 cut(s) 1287
HaeII RGCGCY 1 cut(s) 1535
HaeIII GGCC 4 cut(s) 496, 659, 1237, 1556
HapII CCGG 1 cut(s) 32
HgaI GACGC 1 cut(s) 677
HhaI GCGC 1 cut(s) 1534
Hin6I GCGC 1 cut(s) 1532
HinP1I GCGC 1 cut(s) 1532
HincII GTYRAC 2 cut(s) 103, 1102
HindII GTYRAC 2 cut(s) 103, 1102
HindIII AAGCTT 2 cut(s) 893, 1346
HinfI GANTC 6 cut(s) 323, 480, 615, 1060, 1224, 1331
HpaII CCGG 1 cut(s) 32
HphI GGTGA 4 cut(s) 350, 536, 962, 1148
Hpy166II GTNNAC 3 cut(s) 103, 277, 1102
Hpy188I TCNGA 6 cut(s) 402, 442, 1059, 1148, 1175, 1229
Hpy188III TCNNGA 6 cut(s) 150, 877, 899, 1266, 1304, 1403
Hpy8I GTNNAC 3 cut(s) 103, 277, 1102
HpyAV CCTTC 4 cut(s) 605, 876, 921, 1346
HpyCH4III ACNGT 4 cut(s) 19, 574, 1003, 1414
HpyCH4IV ACGT 1 cut(s) 679
HpyF10VI GCNNNNNNNGC 8 cut(s) 173, 752, 962, 965, 971, 1211, 1531, 1559
HpyF3I CTNAG 5 cut(s) 364, 441, 498, 705, 1172
HpySE526I ACGT 1 cut(s) 679
HspAI GCGC 1 cut(s) 1532
Ksp22I TGATCA 1 cut(s) 651
KspI CCGCGG 1 cut(s) 1554
Kzo9I GATC 2 cut(s) 60, 651
LguI GCTCTTC 1 cut(s) 174
LmnI GCTCC 2 cut(s) 164, 1522
LweI GCATC 9 cut(s) 286, 427, 458, 519, 882, 943, 983, 1081, 1201
MaeI CTAG 3 cut(s) 54, 801, 1570
MaeII ACGT 1 cut(s) 679
MaeIII GTNAC 2 cut(s) 19, 1078
MalI GATC 2 cut(s) 62, 653
MbiI CCGCTC 1 cut(s) 862
MboI GATC 2 cut(s) 60, 651
MboII GAAGA 9 cut(s) 161, 565, 914, 1124, 1172, 1220, 1254, 1405, 1529
MfeI CAATTG 2 cut(s) 978, 1185
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 7 cut(s) 133, 169, 605, 1173, 1363, 1402, 1551
MluCI AATT 8 cut(s) 195, 219, 311, 484, 978, 1007, 1119, 1185
MmeI TCCRAC 3 cut(s) 380, 976, 1252
Mph1103I ATGCAT 1 cut(s) 451
MroXI GAANNNNTTC 2 cut(s) 195, 1119
MseI TTAA 3 cut(s) 110, 144, 1218
MslI CAYNNNNRTG 1 cut(s) 1046
MspA1I CMGCKG 2 cut(s) 752, 1553
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 5 cut(s) 33, 423, 579, 1233, 1239
MunI CAATTG 2 cut(s) 978, 1185
Mva1269I GAATGC 2 cut(s) 451, 1196
MvaI CCWGG 4 cut(s) 423, 579, 1233, 1239
MvnI CGCG 2 cut(s) 231, 1553
MwoI GCNNNNNNNGC 8 cut(s) 173, 752, 962, 965, 971, 1211, 1531, 1559
NciI CCSGG 1 cut(s) 33
NdeII GATC 2 cut(s) 60, 651
NlaIV GGNNCC 8 cut(s) 887, 1000, 1030, 1273, 1317, 1362, 1428, 1548
NmuCI GTSAC 2 cut(s) 19, 1078
NotI GCGGCCGC 1 cut(s) 1554
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 2 cut(s) 183, 688
NspV TTCGAA 2 cut(s) 238, 1282
PaeI GCATGC 1 cut(s) 688
PaqCI CACCTGC 1 cut(s) 551
PciI ACATGT 1 cut(s) 179
PciSI GCTCTTC 1 cut(s) 174
PcsI WCGNNNNNNNCGW 2 cut(s) 235, 685
PctI GAATGC 2 cut(s) 451, 1196
PdmI GAANNNNTTC 2 cut(s) 195, 1119
PfeI GAWTC 6 cut(s) 323, 480, 615, 1060, 1224, 1331
PflMI CCANNNNNTGG 3 cut(s) 253, 428, 1543
PfoI TCCNGGA 1 cut(s) 421
PpuMI RGGWCCY 1 cut(s) 1271
PscI ACATGT 1 cut(s) 179
PsiI TTATAA 1 cut(s) 1418
Psp124BI GAGCTC 1 cut(s) 169
Psp5II RGGWCCY 1 cut(s) 1271
Psp6I CCWGG 4 cut(s) 421, 577, 1231, 1237
PspGI CCWGG 4 cut(s) 421, 577, 1231, 1237
PspN4I GGNNCC 8 cut(s) 887, 1000, 1030, 1273, 1317, 1362, 1428, 1548
PspPI GGNCC 3 cut(s) 494, 1235, 1271
PspPPI RGGWCCY 1 cut(s) 1271
PstI CTGCAG 1 cut(s) 751
PsuI RGATCY 1 cut(s) 60
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 4 cut(s) 362, 409, 576, 788
RsaNI GTAC 4 cut(s) 361, 408, 575, 787
RseI CAYNNNNRTG 1 cut(s) 1046
SacI GAGCTC 1 cut(s) 169
SacII CCGCGG 1 cut(s) 1554
SapI GCTCTTC 1 cut(s) 174
SaqAI TTAA 3 cut(s) 110, 144, 1218
Sau3AI GATC 2 cut(s) 60, 651
Sau96I GGNCC 3 cut(s) 494, 1235, 1271
ScrFI CCNGG 5 cut(s) 33, 423, 579, 1233, 1239
SduI GDGCHC 7 cut(s) 133, 169, 605, 1173, 1363, 1402, 1551
SfaNI GCATC 9 cut(s) 286, 427, 458, 519, 882, 943, 983, 1081, 1201
SfcI CTRYAG 2 cut(s) 596, 747
Sfr303I CCGCGG 1 cut(s) 1554
SfuI TTCGAA 2 cut(s) 238, 1282
SgrBI CCGCGG 1 cut(s) 1554
SinI GGWCC 1 cut(s) 1271
SmiMI CAYNNNNRTG 1 cut(s) 1046
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
SphI GCATGC 1 cut(s) 688
Sse9I AATT 8 cut(s) 195, 219, 311, 484, 978, 1007, 1119, 1185
SsiI CCGC 7 cut(s) 64, 862, 937, 1551, 1553, 1557, 1560
SspMI CTAG 3 cut(s) 54, 801, 1570
SstI GAGCTC 1 cut(s) 169
StyD4I CCNGG 5 cut(s) 31, 421, 577, 1231, 1237
TaaI ACNGT 4 cut(s) 19, 574, 1003, 1414
TaiI ACGT 1 cut(s) 682
TaqI TCGA 2 cut(s) 238, 1282
TasI AATT 8 cut(s) 195, 219, 311, 484, 978, 1007, 1119, 1185
TatI WGTACW 2 cut(s) 407, 786
TauI GCSGC 3 cut(s) 1556, 1559, 1562
TfiI GAWTC 6 cut(s) 323, 480, 615, 1060, 1224, 1331
Tru1I TTAA 3 cut(s) 110, 144, 1218
Tru9I TTAA 3 cut(s) 110, 144, 1218
TscAI CASTG 1 cut(s) 1417
TseFI GTSAC 2 cut(s) 19, 1078
Tsp45I GTSAC 2 cut(s) 19, 1078
TspDTI ATGAA 8 cut(s) 161, 293, 566, 1030, 1064, 1112, 1406, 1530
TspGWI ACGGA 1 cut(s) 503
TspRI CASTG 1 cut(s) 1417
Van91I CCANNNNNTGG 3 cut(s) 253, 428, 1543
VpaK11BI GGWCC 1 cut(s) 1271
XagI CCTNNNNNAGG 1 cut(s) 1236
XapI RAATTY 3 cut(s) 195, 1007, 1119
XceI RCATGY 2 cut(s) 183, 688
XmnI GAANNNNTTC 2 cut(s) 195, 1119
XspI CTAG 3 cut(s) 54, 801, 1570
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.