RLG00000024585

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004128
Physical Location & Seq
Reverse (-)
37256998 .. 37262874
5877 bp
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UTR
Exon/CDS
Intron
RLM00000024585

Sequence Viewer

Length: 336 bp
ATGTGGCATATTCAGCTGTCAAGGCTACCATCTGCTGTTCAAATTCTTGATGGATGTGATGCTTATGATCTTAATGGATTCTTATCTGAAGAGAGAAAACTGGGGTTTGAGGACGCATCGGAAAAGAGCAATGGAAGAGGTCAATCTTCATTCTCGCTTCCACCATACAAATGTCATAAAGGTAGAATAACAGCTTACAAACCCTCCGGAGGTCCATTTTTTAGAATGGATAGTCATGTTTATCCTGATTATGTGGTTCCTCCAAACTATGATTCCCTTCTTGGAAAGCCATCATTGACAGGGATAGGGACTTGCTATAATGAGTTTTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

112

Amino Acids

12.37

Weight (kDa)

6.26

Isoelectric Point (pI)

54.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_C PF02785 59 - 96 6.5e-08 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 206
AcsI RAATTY 1 cut(s) 42
AcuI CTGAAG 1 cut(s) 108
AfiI CCNNNNNNNGG 1 cut(s) 209
AgsI TTSAA 1 cut(s) 41
AjuI GAANNNNNNNTTGG 2 cut(s) 256, 288
AluBI AGCT 2 cut(s) 16, 194
AluI AGCT 2 cut(s) 16, 194
Aor13HI TCCGGA 1 cut(s) 206
ApoI RAATTY 1 cut(s) 42
AspS9I GGNCC 1 cut(s) 212
AvaII GGWCC 1 cut(s) 212
BccI CCATC 3 cut(s) 37, 44, 298
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 1 cut(s) 258
BmrI ACTGGG 1 cut(s) 110
BmsI GCATC 2 cut(s) 49, 125
BmuI ACTGGG 1 cut(s) 110
BsaBI GATNNNNATC 1 cut(s) 82
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 136
Bse8I GATNNNNATC 1 cut(s) 82
BseAI TCCGGA 1 cut(s) 206
BseGI GGATG 1 cut(s) 59
BseJI GATNNNNATC 1 cut(s) 82
BseLI CCNNNNNNNGG 1 cut(s) 209
BseMI GCAATG 1 cut(s) 136
BseNI ACTGG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 209
BsmFI GGGAC 1 cut(s) 322
Bsp13I TCCGGA 1 cut(s) 206
Bsp143I GATC 1 cut(s) 67
BspEI TCCGGA 1 cut(s) 206
BspLI GGNNCC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 136
BsrI ACTGG 1 cut(s) 105
BssMI GATC 1 cut(s) 67
Bst6I CTCTTC 2 cut(s) 84, 130
BstF5I GGATG 1 cut(s) 59
BstKTI GATC 1 cut(s) 70
BstMBI GATC 1 cut(s) 67
BstMWI GCNNNNNNNGC 2 cut(s) 13, 22
BtsCI GGATG 1 cut(s) 59
Cfr13I GGNCC 1 cut(s) 212
CseI GACGC 1 cut(s) 122
CviAII CATG 1 cut(s) 236
CviJI RGCY 4 cut(s) 16, 25, 194, 289
CviKI_1 RGCY 4 cut(s) 16, 25, 194, 289
DpnI GATC 1 cut(s) 69
DpnII GATC 1 cut(s) 67
Eam1104I CTCTTC 2 cut(s) 84, 130
EarI CTCTTC 2 cut(s) 84, 130
Eco47I GGWCC 1 cut(s) 212
Eco57I CTGAAG 1 cut(s) 108
FaeI CATG 1 cut(s) 239
FaiI YATR 8 cut(s) 9, 66, 166, 177, 237, 252, 270, 318
FaqI GGGAC 1 cut(s) 322
FatI CATG 1 cut(s) 235
FokI GGATG 1 cut(s) 66
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 122
Hin1II CATG 1 cut(s) 239
HinfI GANTC 2 cut(s) 78, 272
HpaII CCGG 1 cut(s) 207
Hpy188I TCNGA 2 cut(s) 88, 121
Hpy188III TCNNGA 3 cut(s) 47, 207, 245
HpyAV CCTTC 1 cut(s) 287
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 22
Hsp92II CATG 1 cut(s) 239
Kpn2I TCCGGA 1 cut(s) 206
Kzo9I GATC 1 cut(s) 67
LpnPI CCDG 4 cut(s) 86, 220, 258, 285
LweI GCATC 2 cut(s) 49, 125
MalI GATC 1 cut(s) 69
MboI GATC 1 cut(s) 67
MboII GAAGA 3 cut(s) 101, 138, 147
MluCI AATT 1 cut(s) 42
MnlI CCTC 5 cut(s) 103, 131, 203, 214, 270
MroI TCCGGA 1 cut(s) 206
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 1 cut(s) 169
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 1 cut(s) 207
MwoI GCNNNNNNNGC 2 cut(s) 13, 22
NdeII GATC 1 cut(s) 67
NlaIII CATG 1 cut(s) 239
NlaIV GGNNCC 1 cut(s) 258
PfeI GAWTC 2 cut(s) 78, 272
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 1 cut(s) 212
PvuII CAGCTG 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 169
SaqAI TTAA 1 cut(s) 72
Sau3AI GATC 1 cut(s) 67
Sau96I GGNCC 1 cut(s) 212
SetI ASST 5 cut(s) 18, 142, 184, 196, 214
SfaNI GCATC 2 cut(s) 49, 125
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 169
Sse9I AATT 1 cut(s) 42
TasI AATT 1 cut(s) 42
TfiI GAWTC 2 cut(s) 78, 272
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TspDTI ATGAA 1 cut(s) 138
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.