Rh4AG080200

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004128
Physical Location & Seq
Reverse (-)
16718945 .. 16725329
6385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG080200.1

Sequence Viewer

Length: 1119 bp
ATGGTGGGGAATAAAGTTAGCTTTTTAAGGCAGAGAGCTCAGGGCGCTCAGATTAGTTATAGATCAAGGAAGCACGCGGGAGCTCTCCATGCTACGTGTCTTGCTGATAAAATCCTGGTGGCAAATAGAGGAGAAATTGCTGTTCGTGTTATTCGAACGGCGCATGAGATGGGCATTCCCTGTGTGGCTGTTTACTCAACAATAGACAAGGATGCACTTCATGTCAAATTGGCTGATGAATCAGTTTGTATTGGTGAAGCAGCAAGCAGTCAATCGTACCTAGTGGTTCCAAATGTTTTATCTGCTGCCATCAGTCGTAAATGTACAATGCTTCATCCTGGATATGGTTTCCTGTCTGAGAATGCATCTTTTGTTGAAATGTGCAAAGAACATGGAATCAACTTTATTGGTCCTAAGCCCGAAAGTATCCGTATTATGGGTGATAAAGCGACTGCCAGAGAAACAATGAAGCAAGCAGGTGTTCCAACTGTACCAGGAAGTGATGGATTACTACAGAGCACAGAGGAAGGAATCAAGCTTGCCGATGAGATTGGTTATCCTGTTATGATCAAGGCAACAGCAGGTGGTGGAGGACGTGGTATGCGTCTTGCTAAAGAGCCTGAAGAGTTTGTAAAGTTGCTACAGGCAGCAAAGAGCGAGGCTGTAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTATGTCGTGAATCCTAGACACATTGAATTTCAGGTTCTTGCAGATAAGTATGGTAATGTTGTTCACTTTGGAGAGCGTGATTGTAGCATCCAGAGAAGGAACCAAAAGCTGTTAGAAGAAGCACCCTCTCCTGCATTGACCCCAGAATTGCGAAAAGCCATGGGTGATGCAGCAGTTGCTGCCGCAGCATCTATTGGTTATATTGGTGTTGGAACCATTGAGTTCCTTTTGGACGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTAGGATTCAGGTTGAACATCCTGTGACAGAAATGATTTCCTCTGTTGACTTGATTGAAGAACAAATTCGTGTTGCTATGGGAGAAAAACTCCGATACACACAGGTATATAACGATTTTCAGTTCACACAGTTATCTATAGTAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

372

Amino Acids

40.56

Weight (kDa)

6.34

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 36 - 143 5.5e-42 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 148 - 355 3.9e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 157 - 325 1.2e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 175 - 324 4.1e-07 D-ala D-ala ligase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 467, 572
Acc36I ACCTGC 2 cut(s) 467, 572
AccII CGCG 1 cut(s) 77
AciI CCGC 2 cut(s) 77, 885
AcsI RAATTY 2 cut(s) 728, 1035
AcuI CTGAAG 1 cut(s) 642
AfaI GTAC 3 cut(s) 278, 325, 492
AfiI CCNNNNNNNGG 2 cut(s) 344, 436
AflIII ACRYGT 1 cut(s) 95
AgsI TTSAA 4 cut(s) 377, 728, 986, 1028
AjiI CACGTC 1 cut(s) 596
AjnI CCWGG 3 cut(s) 114, 337, 493
AluBI AGCT 6 cut(s) 21, 38, 83, 538, 668, 811
AluI AGCT 6 cut(s) 21, 38, 83, 538, 668, 811
Alw21I GWGCWC 3 cut(s) 40, 85, 521
AlwNI CAGNNNCTG 1 cut(s) 881
ApeKI GCWGC 7 cut(s) 260, 305, 647, 668, 872, 881, 887
ApoI RAATTY 2 cut(s) 728, 1035
Asp700I GAANNNNTTC 1 cut(s) 1035
AspLEI GCGC 2 cut(s) 47, 163
AspS9I GGNCC 1 cut(s) 410
AsuHPI GGTGA 3 cut(s) 266, 452, 878
AsuII TTCGAA 1 cut(s) 154
AvaII GGWCC 1 cut(s) 410
BanII GRGCYC 2 cut(s) 40, 85
Bbv12I GWGCWC 3 cut(s) 40, 85, 521
BbvI GCAGC 7 cut(s) 272, 292, 655, 659, 868, 884, 899
BccI CCATC 4 cut(s) 163, 317, 497, 677
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 3 cut(s) 116, 339, 495
BciVI GTATCC 1 cut(s) 437
BclI TGATCA 1 cut(s) 567
BfaI CTAG 3 cut(s) 281, 717, 972
BfmI CTRYAG 4 cut(s) 512, 641, 663, 1107
BfoI RGCGCY 1 cut(s) 48
BfuAI ACCTGC 2 cut(s) 467, 572
BfuI GTATCC 1 cut(s) 437
BisI GCNGC 8 cut(s) 261, 306, 648, 669, 873, 882, 885, 888
BlsI GCNGC 8 cut(s) 262, 307, 649, 670, 874, 883, 886, 889
Bme1390I CCNGG 3 cut(s) 116, 339, 495
Bme18I GGWCC 1 cut(s) 410
BmgBI CACGTC 1 cut(s) 596
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 4 cut(s) 288, 803, 916, 946
BmrFI CCNGG 3 cut(s) 116, 339, 495
BmsI GCATC 5 cut(s) 202, 374, 798, 859, 899
BplI GAGNNNNNCTC 2 cut(s) 1044, 1076
Bpu10I CCTNAGC 2 cut(s) 39, 414
Bpu14I TTCGAA 1 cut(s) 154
BsaAI YACGTR 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 861
Bsc4I CCNNNNNNNGG 2 cut(s) 344, 436
BseBI CCWGG 3 cut(s) 116, 339, 495
BseDI CCNNGG 1 cut(s) 861
BseGI GGATG 4 cut(s) 217, 334, 789, 988
BseLI CCNNNNNNNGG 2 cut(s) 344, 436
BseMII CTCAG 3 cut(s) 53, 62, 348
BseRI GAGGAG 1 cut(s) 144
BseXI GCAGC 7 cut(s) 272, 292, 655, 659, 868, 884, 899
Bsh1236I CGCG 1 cut(s) 77
BsiHKAI GWGCWC 3 cut(s) 40, 85, 521
BslI CCNNNNNNNGG 2 cut(s) 344, 436
BsmI GAATGC 2 cut(s) 174, 367
Bsp119I TTCGAA 1 cut(s) 154
Bsp1286I GDGCHC 3 cut(s) 40, 85, 521
Bsp1407I TGTACA 1 cut(s) 323
Bsp143I GATC 2 cut(s) 62, 567
Bsp19I CCATGG 1 cut(s) 861
BspACI CCGC 2 cut(s) 77, 885
BspCNI CTCAG 3 cut(s) 52, 61, 349
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 4 cut(s) 288, 803, 916, 946
BspMI ACCTGC 2 cut(s) 467, 572
BspT104I TTCGAA 1 cut(s) 154
BsrGI TGTACA 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 861
BssMI GATC 2 cut(s) 62, 567
BssT1I CCWWGG 1 cut(s) 861
Bst2UI CCWGG 3 cut(s) 116, 339, 495
Bst4CI ACNGT 2 cut(s) 490, 1101
Bst6I CTCTTC 1 cut(s) 618
BstAPI GCANNNNNTGC 2 cut(s) 878, 881
BstAUI TGTACA 1 cut(s) 323
BstBAI YACGTR 1 cut(s) 96
BstBI TTCGAA 1 cut(s) 154
BstC8I GCNNGC 4 cut(s) 75, 265, 474, 540
BstDEI CTNAG 4 cut(s) 39, 48, 357, 414
BstDSI CCRYGG 1 cut(s) 861
BstF5I GGATG 4 cut(s) 217, 334, 789, 988
BstFNI CGCG 1 cut(s) 77
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 2 cut(s) 47, 163
BstKTI GATC 2 cut(s) 65, 570
BstMBI GATC 2 cut(s) 62, 567
BstMWI GCNNNNNNNGC 6 cut(s) 44, 89, 668, 878, 881, 887
BstNI CCWGG 3 cut(s) 116, 339, 495
BstSCI CCNGG 3 cut(s) 114, 337, 493
BstSFI CTRYAG 4 cut(s) 512, 641, 663, 1107
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 7 cut(s) 272, 292, 655, 659, 868, 884, 899
BsuI GTATCC 1 cut(s) 437
BtgI CCRYGG 1 cut(s) 861
BtrI CACGTC 1 cut(s) 596
BtsCI GGATG 4 cut(s) 217, 334, 789, 988
BveI ACCTGC 2 cut(s) 467, 572
Cac8I GCNNGC 4 cut(s) 75, 265, 474, 540
CaiI CAGNNNCTG 1 cut(s) 881
CfoI GCGC 2 cut(s) 47, 163
Cfr13I GGNCC 1 cut(s) 410
CseI GACGC 1 cut(s) 593
Csp6I GTAC 3 cut(s) 277, 324, 491
CviAII CATG 6 cut(s) 89, 164, 221, 392, 862, 958
CviQI GTAC 3 cut(s) 277, 324, 491
DdeI CTNAG 4 cut(s) 39, 48, 357, 414
DpnI GATC 2 cut(s) 64, 569
DpnII GATC 2 cut(s) 62, 567
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Ecl136II GAGCTC 2 cut(s) 38, 83
Eco130I CCWWGG 1 cut(s) 861
Eco24I GRGCYC 2 cut(s) 40, 85
Eco47I GGWCC 1 cut(s) 410
Eco53kI GAGCTC 2 cut(s) 38, 83
Eco57I CTGAAG 1 cut(s) 642
EcoICRI GAGCTC 2 cut(s) 38, 83
EcoRII CCWGG 3 cut(s) 114, 337, 493
EcoT14I CCWWGG 1 cut(s) 861
EcoT22I ATGCAT 1 cut(s) 367
EcoT38I GRGCYC 2 cut(s) 40, 85
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 6 cut(s) 92, 167, 224, 395, 865, 961
FatI CATG 6 cut(s) 88, 163, 220, 391, 861, 957
FauI CCCGC 1 cut(s) 70
FbaI TGATCA 1 cut(s) 567
Fnu4HI GCNGC 8 cut(s) 261, 306, 648, 669, 873, 882, 885, 888
FokI GGATG 4 cut(s) 224, 321, 776, 975
FriOI GRGCYC 2 cut(s) 40, 85
Fsp4HI GCNGC 8 cut(s) 261, 306, 648, 669, 873, 882, 885, 888
FspBI CTAG 3 cut(s) 281, 717, 972
GlaI GCGC 2 cut(s) 46, 162
GluI GCNGC 8 cut(s) 261, 306, 648, 669, 873, 882, 885, 888
HaeII RGCGCY 1 cut(s) 48
HgaI GACGC 1 cut(s) 593
HhaI GCGC 2 cut(s) 47, 163
Hin1II CATG 6 cut(s) 92, 167, 224, 395, 865, 961
Hin6I GCGC 2 cut(s) 45, 161
HinP1I GCGC 2 cut(s) 45, 161
HincII GTYRAC 1 cut(s) 1018
HindII GTYRAC 1 cut(s) 1018
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 5 cut(s) 239, 396, 531, 712, 976
HphI GGTGA 3 cut(s) 266, 452, 878
Hpy166II GTNNAC 4 cut(s) 193, 766, 1018, 1095
Hpy188I TCNGA 3 cut(s) 51, 358, 1064
Hpy188III TCNNGA 2 cut(s) 709, 793
Hpy8I GTNNAC 4 cut(s) 193, 766, 1018, 1095
HpyAV CCTTC 2 cut(s) 521, 792
HpyCH4III ACNGT 2 cut(s) 490, 1101
HpyCH4IV ACGT 2 cut(s) 95, 595
HpyCH4V TGCA 6 cut(s) 215, 365, 384, 743, 836, 872
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 89, 668, 878, 881, 887
HpyF3I CTNAG 4 cut(s) 39, 48, 357, 414
HpySE526I ACGT 2 cut(s) 95, 595
Hsp92II CATG 6 cut(s) 92, 167, 224, 395, 865, 961
HspAI GCGC 2 cut(s) 45, 161
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 2 cut(s) 62, 567
LmnI GCTCC 1 cut(s) 80
Lsp1109I GCAGC 7 cut(s) 272, 292, 655, 659, 868, 884, 899
LweI GCATC 5 cut(s) 202, 374, 798, 859, 899
MaeI CTAG 3 cut(s) 281, 717, 972
MaeII ACGT 2 cut(s) 95, 595
MaeIII GTNAC 1 cut(s) 994
MalI GATC 2 cut(s) 64, 569
MboI GATC 2 cut(s) 62, 567
MboII GAAGA 3 cut(s) 635, 830, 1040
MhlI GDGCHC 3 cut(s) 40, 85, 521
MluCI AATT 5 cut(s) 135, 227, 728, 848, 1035
MmeI TCCRAC 2 cut(s) 509, 892
MnlI CCTC 7 cut(s) 122, 517, 584, 652, 838, 935, 1021
Mph1103I ATGCAT 1 cut(s) 367
MroXI GAANNNNTTC 1 cut(s) 1035
MseI TTAA 1 cut(s) 26
MslI CAYNNNNRTG 1 cut(s) 962
MspR9I CCNGG 3 cut(s) 116, 339, 495
Mva1269I GAATGC 2 cut(s) 174, 367
MvaI CCWGG 3 cut(s) 116, 339, 495
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 6 cut(s) 44, 89, 668, 878, 881, 887
NcoI CCATGG 1 cut(s) 861
NdeII GATC 2 cut(s) 62, 567
NlaIII CATG 6 cut(s) 92, 167, 224, 395, 865, 961
NlaIV GGNNCC 4 cut(s) 288, 803, 916, 946
NmuCI GTSAC 1 cut(s) 994
NsiI ATGCAT 1 cut(s) 367
NspV TTCGAA 1 cut(s) 154
PaqCI CACCTGC 2 cut(s) 467, 572
PcsI WCGNNNNNNNCGW 2 cut(s) 151, 601
PctI GAATGC 2 cut(s) 174, 367
PdmI GAANNNNTTC 1 cut(s) 1035
PfeI GAWTC 5 cut(s) 239, 396, 531, 712, 976
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 8 cut(s) 262, 307, 649, 670, 874, 883, 886, 889
Ppu21I YACGTR 1 cut(s) 96
Psp124BI GAGCTC 2 cut(s) 40, 85
Psp6I CCWGG 3 cut(s) 114, 337, 493
PspGI CCWGG 3 cut(s) 114, 337, 493
PspN4I GGNNCC 4 cut(s) 288, 803, 916, 946
PspPI GGNCC 1 cut(s) 410
PstNI CAGNNNCTG 1 cut(s) 881
RsaI GTAC 3 cut(s) 278, 325, 492
RsaNI GTAC 3 cut(s) 277, 324, 491
RseI CAYNNNNRTG 1 cut(s) 962
SacI GAGCTC 2 cut(s) 40, 85
SaqAI TTAA 1 cut(s) 26
SatI GCNGC 8 cut(s) 261, 306, 648, 669, 873, 882, 885, 888
Sau3AI GATC 2 cut(s) 62, 567
Sau96I GGNCC 1 cut(s) 410
ScrFI CCNGG 3 cut(s) 116, 339, 495
SduI GDGCHC 3 cut(s) 40, 85, 521
SfaNI GCATC 5 cut(s) 202, 374, 798, 859, 899
SfcI CTRYAG 4 cut(s) 512, 641, 663, 1107
SfuI TTCGAA 1 cut(s) 154
SinI GGWCC 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 962
Sse9I AATT 5 cut(s) 135, 227, 728, 848, 1035
SsiI CCGC 2 cut(s) 77, 885
SspMI CTAG 3 cut(s) 281, 717, 972
SstI GAGCTC 2 cut(s) 40, 85
StyD4I CCNGG 3 cut(s) 114, 337, 493
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 2 cut(s) 490, 1101
TaiI ACGT 2 cut(s) 98, 598
TaqI TCGA 1 cut(s) 154
TasI AATT 5 cut(s) 135, 227, 728, 848, 1035
TatI WGTACW 1 cut(s) 323
TauI GCSGC 1 cut(s) 887
TfiI GAWTC 5 cut(s) 239, 396, 531, 712, 976
Tru1I TTAA 1 cut(s) 26
Tru9I TTAA 1 cut(s) 26
TseFI GTSAC 1 cut(s) 994
TseI GCWGC 7 cut(s) 260, 305, 647, 668, 872, 881, 887
Tsp45I GTSAC 1 cut(s) 994
TspDTI ATGAA 6 cut(s) 209, 252, 323, 482, 946, 980
TspGWI ACGGA 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 410
XapI RAATTY 2 cut(s) 728, 1035
XmnI GAANNNNTTC 1 cut(s) 1035
XspI CTAG 3 cut(s) 281, 717, 972
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.