Rroxscaffold_5G00342540

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004128
Physical Location & Seq
Reverse (-)
11505548 .. 11511110
5563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00342540.1

Sequence Viewer

Length: 1623 bp
ATGGACGCCACCATGCCCATTTGCAAGACTGTTACTCCAACTCCGCGCTTATTTTTTGGGAAAACTGGAGAGATCAGGAGTTCCCAATGTAGCTTTATGGTGGGAAATAAAGTTAGCTTTTTGAGGCAGAGGGCTCAGGGCGCTCAGATTAGTTATAGATCAAGGAAGCATGCCGGAGCTCTCCATGCTACGTGTCTTGCTGATAAAATCCTGGTGGCAAATAGAGGAGAGATTGCTGTTCGTGTTATTCGCACGGCGCATGAGATGGGCATACCCTGTGTGGCTGTTTACTCAACAATCGACAAGGATGCACTTCATGTCAAATTGGCGGATGAATCAGTTTGTATTGGTGAAGCAGCAAGCAGTCAATCGTACCTAGTGGTTCCCAATGTTTTGTCTGCTGCCATCAGTCGTAAATGTACAATGCTTCATCCTGGATATGGTTTCCTGTCTGAGAATGCATCTTTTGTTGAAATGTGCAAAGAACATGGAATCAACTTTATTGGTCCTAAGCCCGAAAGTATCCGTATTATGGGTGATAAAGCGACTGCCAGAGAAACAATGAAGCAAGCAGGTGTTCCAACTGTACCAGGAAGTGATGGATTACTACAGAGCACAGAGGAAGGAATCAAGCTTGCCAATGAGATTGGTTATCCTGTTATGATCAAGGCAACAGCAGGTGGTGGAGGACGTGGTATGCGTCTTGCTAAAGAGCCTGAAGAGTTTGTAAAGTTGCTACAGGCAGCAAAGAGCGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTATGTCGTAAATCCTAGACACATTGAATTTCAGGTTCTTGCAGATAAGTATGGTAATGTTGTTCACTTTGGAGAGCGTGATTGTAGCATCCAGAGAAGGAACCAAAAGCTGTTAGAAGAAGCACCCTCTCCTGCATTGACCCCAGAATTGCGAAAAGCCATGGGTGATGCAGCAGTTGCTGCCGCAGCATCTATTGGTTATATTGGTGTTGGAACCATTGAGTTCCTTTTGGACGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTAGGATTCAGGTTGAACATCCTGTGACAGAAATGATTTCCTCTGTTGACTTGATTGAAGAACAAATTCGTGTTGCTATGGGAGAAAAACTCCGATACACACAGGATGATATCGTGCTCAGAGGGCATTCAATTGAATGTCGTATCAATGCAGAAGATGCTTTTAAAGGATTCCGACCTGGGCCAGGTAGAATAACAGCTTACTTACCCGCCGGAGGTCCATTTGTTAGAATGGATAGTCATGTTTATCCTGATTATGTGGTTCCTCCAAACTATGATTCCCTTCTTGGAAAGGTTTGTGCCCCAAAGAACCATCTTATTGTTTGGGCACCAACAAGAGAAAAGGCAATTGATCGGATGAAAAGAGCTCTTAACGACACTATTATAACAGGGGTTCCTACAACTATTGAATATCATAAGCTCATCCTGGAGATAGACGATTTCAAAAATGGGAAGGTTGATACTGCTTTTATTCCCAAGCATGAAGAGGAGCTAGCAGCGCCCCAACAAGTGTTGCCTGCATTCCCACCCAAAGAACTAGCTAGTGCAAGTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

540

Amino Acids

59.08

Weight (kDa)

6.93

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 68 - 175 1.1e-41 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 180 - 387 4.5e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 189 - 357 2.9e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 207 - 356 5.4e-07 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 401 - 514 3.9e-34 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1451
AarI CACCTGC 2 cut(s) 563, 668
Acc36I ACCTGC 2 cut(s) 563, 668
AccB1I GGYRCC 1 cut(s) 1393
AccII CGCG 1 cut(s) 46
AciI CCGC 4 cut(s) 44, 329, 981, 1275
AcsI RAATTY 2 cut(s) 824, 1131
AcuI CTGAAG 1 cut(s) 738
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 4 cut(s) 374, 421, 588, 1618
AfiI CCNNNNNNNGG 4 cut(s) 440, 532, 1250, 1280
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 8 cut(s) 473, 824, 1082, 1124, 1197, 1202, 1475, 1510
AjiI CACGTC 1 cut(s) 692
AjnI CCWGG 6 cut(s) 210, 433, 589, 1243, 1249, 1491
AjuI GAANNNNNNNTTGG 2 cut(s) 1327, 1359
Alw21I GWGCWC 4 cut(s) 181, 617, 1185, 1435
AlwNI CAGNNNCTG 1 cut(s) 977
AoxI GGCC 1 cut(s) 1247
ApoI RAATTY 2 cut(s) 824, 1131
Asp700I GAANNNNTTC 1 cut(s) 1131
AspLEI GCGC 4 cut(s) 48, 143, 259, 1567
AspS9I GGNCC 3 cut(s) 506, 1247, 1283
AsuHPI GGTGA 3 cut(s) 362, 548, 974
AsuNHI GCTAGC 1 cut(s) 1558
AvaII GGWCC 2 cut(s) 506, 1283
BaeGI GKGCMC 2 cut(s) 1369, 1396
BanI GGYRCC 1 cut(s) 1393
BanII GRGCYC 3 cut(s) 136, 181, 1435
Bbv12I GWGCWC 4 cut(s) 181, 617, 1185, 1435
BccI CCATC 5 cut(s) 259, 413, 593, 773, 1386
BceAI ACGGC 1 cut(s) 270
BcgI CGANNNNNNTGC 2 cut(s) 290, 324
BciT130I CCWGG 6 cut(s) 212, 435, 591, 1245, 1251, 1493
BciVI GTATCC 1 cut(s) 533
BclI TGATCA 1 cut(s) 663
BfaI CTAG 6 cut(s) 377, 813, 1068, 1559, 1604, 1608
BfmI CTRYAG 3 cut(s) 608, 737, 759
BfoI RGCGCY 2 cut(s) 144, 1568
BfuAI ACCTGC 2 cut(s) 563, 668
BfuI GTATCC 1 cut(s) 533
Bme1390I CCNGG 6 cut(s) 212, 435, 591, 1245, 1251, 1493
Bme18I GGWCC 2 cut(s) 506, 1283
BmgBI CACGTC 1 cut(s) 692
BmgT120I GGNCC 3 cut(s) 506, 1247, 1283
BmiI GGNNCC 7 cut(s) 384, 899, 1012, 1042, 1329, 1395, 1461
BmrFI CCNGG 6 cut(s) 212, 435, 591, 1245, 1251, 1493
BmsI GCATC 6 cut(s) 298, 470, 894, 955, 995, 1213
BmtI GCTAGC 1 cut(s) 1562
BplI GAGNNNNNCTC 2 cut(s) 1140, 1172
BpmI CTGGAG 2 cut(s) 87, 1514
Bpu10I CCTNAGC 2 cut(s) 135, 510
BsaAI YACGTR 2 cut(s) 192, 1620
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 2 cut(s) 957, 1244
BsaXI ACNNNNNCTCC 4 cut(s) 19, 25, 49, 55
Bsc4I CCNNNNNNNGG 4 cut(s) 440, 532, 1250, 1280
Bse1I ACTGG 1 cut(s) 70
BseBI CCWGG 6 cut(s) 212, 435, 591, 1245, 1251, 1493
BseDI CCNNGG 2 cut(s) 957, 1244
BseGI GGATG 8 cut(s) 313, 337, 430, 885, 1084, 1177, 1428, 1488
BseLI CCNNNNNNNGG 4 cut(s) 440, 532, 1250, 1280
BseMII CTCAG 4 cut(s) 149, 158, 444, 1198
BseNI ACTGG 1 cut(s) 70
BseRI GAGGAG 2 cut(s) 240, 1568
BseSI GKGCMC 2 cut(s) 1369, 1396
Bsh1236I CGCG 1 cut(s) 46
BshFI GGCC 1 cut(s) 1249
BshNI GGYRCC 1 cut(s) 1393
BsiHKAI GWGCWC 4 cut(s) 181, 617, 1185, 1435
BsiSI CCGG 2 cut(s) 174, 1278
BslI CCNNNNNNNGG 4 cut(s) 440, 532, 1250, 1280
BsmI GAATGC 3 cut(s) 463, 1192, 1586
BsnI GGCC 1 cut(s) 1249
Bsp1286I GDGCHC 7 cut(s) 136, 181, 617, 1185, 1369, 1396, 1435
Bsp1407I TGTACA 1 cut(s) 419
Bsp143I GATC 4 cut(s) 72, 158, 663, 1417
Bsp19I CCATGG 1 cut(s) 957
BspACI CCGC 4 cut(s) 44, 329, 981, 1275
BspANI GGCC 1 cut(s) 1249
BspCNI CTCAG 4 cut(s) 148, 157, 445, 1197
BspFNI CGCG 1 cut(s) 46
BspLI GGNNCC 7 cut(s) 384, 899, 1012, 1042, 1329, 1395, 1461
BspMAI CTGCAG 1 cut(s) 763
BspMI ACCTGC 2 cut(s) 563, 668
BspOI GCTAGC 1 cut(s) 1562
BspT107I GGYRCC 1 cut(s) 1393
BsrGI TGTACA 1 cut(s) 419
BsrI ACTGG 1 cut(s) 70
BssECI CCNNGG 2 cut(s) 957, 1244
BssMI GATC 4 cut(s) 72, 158, 663, 1417
BssNI GRCGYC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 957
Bst2UI CCWGG 6 cut(s) 212, 435, 591, 1245, 1251, 1493
Bst4CI ACNGT 2 cut(s) 31, 586
Bst6I CTCTTC 2 cut(s) 714, 1545
BstACI GRCGYC 1 cut(s) 6
BstAPI GCANNNNNTGC 3 cut(s) 974, 977, 1223
BstAUI TGTACA 1 cut(s) 419
BstBAI YACGTR 2 cut(s) 192, 1620
BstC8I GCNNGC 6 cut(s) 171, 361, 570, 636, 1560, 1584
BstDEI CTNAG 5 cut(s) 135, 144, 453, 510, 1184
BstDSI CCRYGG 1 cut(s) 957
BstENI CCTNNNNNAGG 1 cut(s) 1248
BstF5I GGATG 8 cut(s) 313, 337, 430, 885, 1084, 1177, 1428, 1488
BstFNI CGCG 1 cut(s) 46
BstH2I RGCGCY 2 cut(s) 144, 1568
BstHHI GCGC 4 cut(s) 48, 143, 259, 1567
BstKTI GATC 4 cut(s) 75, 161, 666, 1420
BstMBI GATC 4 cut(s) 72, 158, 663, 1417
BstMWI GCNNNNNNNGC 9 cut(s) 140, 185, 764, 974, 977, 983, 1189, 1223, 1564
BstNI CCWGG 6 cut(s) 212, 435, 591, 1245, 1251, 1493
BstNSI RCATGY 1 cut(s) 173
BstSCI CCNGG 6 cut(s) 210, 433, 589, 1243, 1249, 1491
BstSFI CTRYAG 3 cut(s) 608, 737, 759
BstSLI GKGCMC 2 cut(s) 1369, 1396
BstSNI TACGTA 1 cut(s) 1620
BstUI CGCG 1 cut(s) 46
BsuI GTATCC 1 cut(s) 533
BsuRI GGCC 1 cut(s) 1249
BtgI CCRYGG 1 cut(s) 957
BtrI CACGTC 1 cut(s) 692
BtsCI GGATG 8 cut(s) 313, 337, 430, 885, 1084, 1177, 1428, 1488
BveI ACCTGC 2 cut(s) 563, 668
Cac8I GCNNGC 6 cut(s) 171, 361, 570, 636, 1560, 1584
CaiI CAGNNNCTG 1 cut(s) 977
CfoI GCGC 4 cut(s) 48, 143, 259, 1567
Cfr13I GGNCC 3 cut(s) 506, 1247, 1283
CseI GACGC 2 cut(s) 14, 689
Csp6I GTAC 4 cut(s) 373, 420, 587, 1617
CviQI GTAC 4 cut(s) 373, 420, 587, 1617
DdeI CTNAG 5 cut(s) 135, 144, 453, 510, 1184
DpnI GATC 4 cut(s) 74, 160, 665, 1419
DpnII GATC 4 cut(s) 72, 158, 663, 1417
DraI TTTAAA 1 cut(s) 1231
Eam1104I CTCTTC 2 cut(s) 714, 1545
EarI CTCTTC 2 cut(s) 714, 1545
EciI GGCGGA 1 cut(s) 344
Ecl136II GAGCTC 2 cut(s) 179, 1433
Eco105I TACGTA 1 cut(s) 1620
Eco130I CCWWGG 1 cut(s) 957
Eco24I GRGCYC 3 cut(s) 136, 181, 1435
Eco32I GATATC 1 cut(s) 1177
Eco47I GGWCC 2 cut(s) 506, 1283
Eco53kI GAGCTC 2 cut(s) 179, 1433
Eco57I CTGAAG 1 cut(s) 738
EcoICRI GAGCTC 2 cut(s) 179, 1433
EcoNI CCTNNNNNAGG 1 cut(s) 1248
EcoRII CCWGG 6 cut(s) 210, 433, 589, 1243, 1249, 1491
EcoRV GATATC 1 cut(s) 1177
EcoT14I CCWWGG 1 cut(s) 957
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 3 cut(s) 136, 181, 1435
ErhI CCWWGG 1 cut(s) 957
FauI CCCGC 1 cut(s) 1282
FbaI TGATCA 1 cut(s) 663
FokI GGATG 8 cut(s) 320, 344, 417, 872, 1071, 1184, 1435, 1475
FriOI GRGCYC 3 cut(s) 136, 181, 1435
FspBI CTAG 6 cut(s) 377, 813, 1068, 1559, 1604, 1608
GlaI GCGC 4 cut(s) 47, 142, 258, 1566
GsuI CTGGAG 2 cut(s) 87, 1514
HaeII RGCGCY 2 cut(s) 144, 1568
HaeIII GGCC 1 cut(s) 1249
HapII CCGG 2 cut(s) 174, 1278
HgaI GACGC 2 cut(s) 14, 689
HhaI GCGC 4 cut(s) 48, 143, 259, 1567
Hin1I GRCGYC 1 cut(s) 6
Hin6I GCGC 4 cut(s) 46, 141, 257, 1565
HinP1I GCGC 4 cut(s) 46, 141, 257, 1565
HincII GTYRAC 1 cut(s) 1114
HindII GTYRAC 1 cut(s) 1114
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 6 cut(s) 335, 492, 627, 1072, 1236, 1343
HpaII CCGG 2 cut(s) 174, 1278
HphI GGTGA 3 cut(s) 362, 548, 974
Hpy166II GTNNAC 3 cut(s) 289, 862, 1114
Hpy188I TCNGA 6 cut(s) 147, 454, 1160, 1187, 1241, 1422
Hpy188III TCNNGA 3 cut(s) 76, 889, 1316
Hpy8I GTNNAC 3 cut(s) 289, 862, 1114
HpyAV CCTTC 4 cut(s) 617, 888, 1358, 1513
HpyCH4III ACNGT 2 cut(s) 31, 586
HpyCH4IV ACGT 3 cut(s) 191, 691, 1619
HpyF10VI GCNNNNNNNGC 9 cut(s) 140, 185, 764, 974, 977, 983, 1189, 1223, 1564
HpyF3I CTNAG 5 cut(s) 135, 144, 453, 510, 1184
HpySE526I ACGT 3 cut(s) 191, 691, 1619
Hsp92I GRCGYC 1 cut(s) 6
HspAI GCGC 4 cut(s) 46, 141, 257, 1565
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 4 cut(s) 72, 158, 663, 1417
LmnI GCTCC 2 cut(s) 176, 1555
LweI GCATC 6 cut(s) 298, 470, 894, 955, 995, 1213
MaeI CTAG 6 cut(s) 377, 813, 1068, 1559, 1604, 1608
MaeII ACGT 3 cut(s) 191, 691, 1619
MaeIII GTNAC 2 cut(s) 31, 1090
MalI GATC 4 cut(s) 74, 160, 665, 1419
MboI GATC 4 cut(s) 72, 158, 663, 1417
MboII GAAGA 5 cut(s) 731, 926, 1136, 1232, 1562
MfeI CAATTG 2 cut(s) 1197, 1413
MhlI GDGCHC 7 cut(s) 136, 181, 617, 1185, 1369, 1396, 1435
MluCI AATT 6 cut(s) 323, 824, 944, 1131, 1197, 1413
MmeI TCCRAC 4 cut(s) 62, 605, 988, 1264
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 1131
MseI TTAA 2 cut(s) 1230, 1437
MslI CAYNNNNRTG 1 cut(s) 1058
MspA1I CMGCKG 1 cut(s) 764
MspI CCGG 2 cut(s) 174, 1278
MspR9I CCNGG 6 cut(s) 212, 435, 591, 1245, 1251, 1493
MunI CAATTG 2 cut(s) 1197, 1413
Mva1269I GAATGC 3 cut(s) 463, 1192, 1586
MvaI CCWGG 6 cut(s) 212, 435, 591, 1245, 1251, 1493
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 9 cut(s) 140, 185, 764, 974, 977, 983, 1189, 1223, 1564
NcoI CCATGG 1 cut(s) 957
NdeII GATC 4 cut(s) 72, 158, 663, 1417
NheI GCTAGC 1 cut(s) 1558
NlaIV GGNNCC 7 cut(s) 384, 899, 1012, 1042, 1329, 1395, 1461
NmuCI GTSAC 1 cut(s) 1090
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 1 cut(s) 173
PaeI GCATGC 1 cut(s) 173
PaqCI CACCTGC 2 cut(s) 563, 668
PcsI WCGNNNNNNNCGW 1 cut(s) 697
PctI GAATGC 3 cut(s) 463, 1192, 1586
PdmI GAANNNNTTC 1 cut(s) 1131
PfeI GAWTC 6 cut(s) 335, 492, 627, 1072, 1236, 1343
PfoI TCCNGGA 2 cut(s) 433, 1491
Ppu21I YACGTR 2 cut(s) 192, 1620
PsiI TTATAA 1 cut(s) 1451
Psp124BI GAGCTC 2 cut(s) 181, 1435
Psp6I CCWGG 6 cut(s) 210, 433, 589, 1243, 1249, 1491
PspGI CCWGG 6 cut(s) 210, 433, 589, 1243, 1249, 1491
PspN4I GGNNCC 7 cut(s) 384, 899, 1012, 1042, 1329, 1395, 1461
PspPI GGNCC 3 cut(s) 506, 1247, 1283
PstI CTGCAG 1 cut(s) 763
PstNI CAGNNNCTG 1 cut(s) 977
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 4 cut(s) 374, 421, 588, 1618
RsaNI GTAC 4 cut(s) 373, 420, 587, 1617
RseI CAYNNNNRTG 1 cut(s) 1058
SacI GAGCTC 2 cut(s) 181, 1435
SaqAI TTAA 2 cut(s) 1230, 1437
Sau3AI GATC 4 cut(s) 72, 158, 663, 1417
Sau96I GGNCC 3 cut(s) 506, 1247, 1283
ScrFI CCNGG 6 cut(s) 212, 435, 591, 1245, 1251, 1493
SduI GDGCHC 7 cut(s) 136, 181, 617, 1185, 1369, 1396, 1435
SfaNI GCATC 6 cut(s) 298, 470, 894, 955, 995, 1213
SfcI CTRYAG 3 cut(s) 608, 737, 759
SinI GGWCC 2 cut(s) 506, 1283
SmiMI CAYNNNNRTG 1 cut(s) 1058
SnaBI TACGTA 1 cut(s) 1620
SphI GCATGC 1 cut(s) 173
Sse9I AATT 6 cut(s) 323, 824, 944, 1131, 1197, 1413
SsiI CCGC 4 cut(s) 44, 329, 981, 1275
SspMI CTAG 6 cut(s) 377, 813, 1068, 1559, 1604, 1608
SstI GAGCTC 2 cut(s) 181, 1435
StyD4I CCNGG 6 cut(s) 210, 433, 589, 1243, 1249, 1491
StyI CCWWGG 1 cut(s) 957
TaaI ACNGT 2 cut(s) 31, 586
TaiI ACGT 3 cut(s) 194, 694, 1622
TaqI TCGA 1 cut(s) 300
TasI AATT 6 cut(s) 323, 824, 944, 1131, 1197, 1413
TatI WGTACW 1 cut(s) 419
TauI GCSGC 1 cut(s) 983
TfiI GAWTC 6 cut(s) 335, 492, 627, 1072, 1236, 1343
Tru1I TTAA 2 cut(s) 1230, 1437
Tru9I TTAA 2 cut(s) 1230, 1437
TseFI GTSAC 1 cut(s) 1090
Tsp45I GTSAC 1 cut(s) 1090
TspDTI ATGAA 8 cut(s) 305, 348, 419, 578, 1042, 1076, 1439, 1563
TspGWI ACGGA 1 cut(s) 515
VpaK11BI GGWCC 2 cut(s) 506, 1283
XagI CCTNNNNNAGG 1 cut(s) 1248
XapI RAATTY 2 cut(s) 824, 1131
XceI RCATGY 1 cut(s) 173
XmnI GAANNNNTTC 1 cut(s) 1131
XspI CTAG 6 cut(s) 377, 813, 1068, 1559, 1604, 1608
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.