Prupe.4G259200_v2.0.a1

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0004128
Physical Location & Seq
Reverse (-)
18596908 .. 18603850
6943 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G259200.1

Sequence Viewer

Length: 1602 bp
ATGGATGCCACAATGCCCATTTGCAAGATTGTCACTCCAACTCCTGGCTTATTTTTTGGGAGAACTAGAGAGATCCAGAGTTCCCAATGCAGCTTTATGGTGGGAAATAAAGTCAACTTTTTAAGGCAGAGAGCTCGGGGTGCTCAAGTTACTTTCAAATCCAGGAAGCATGGGGGAGCTCTCCATGCTACATGTCGTGCTGAAAAAATTCTGGTGGCAAATAGGGGAGAAATTGCTGTTCGTATTATTCGAACTGCCCATGAAATGGGAATACCTTGTGTGGCTGTTTACTCAACAATAGACAAGGACGCACTTCATGTGAAATTGGCTGATGAATCAGTTTGTATTGGTGAAGCAGCAAGCAGTCAGTCGTACTTAGTTGTTCCAAATGTTTTATCTGCTGCTATCAGTCGGAAATGTACAATGCTGCATCCAGGATACGGTTTCCTTGCTGAGAATGCATCATTTGTTGAAATGTGCAGAGAACACGGAATCAACTTTATTGGGCCTAAGCCCGACAGCATCCGTGTTATGGGTGATAAAGCGACTGCCAGAGACACAATGAAGACAGCAGGTGTTCCAACTGTACCAGGAAGTGATGGATTACTACAGAGCACAGAGGAAGGAATCAGGCTTGCAGATGAGATTGGTTTTCCTGTTATGATCAAGGCAACGGCAGGAGGTGGAGGACGTGGCATGCGTCTTGCTAAAGAACCTGAAGAGTTTGTGAAGTTGTTACAGCAAGCAAAGAGTGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTTTACTTGGAAAAGTACATCCAAAATCCTAGACACATTGAATTTCAGGTTCTTGCAGATAAATATGGTAATGTTGTTCACTTTGGAGAGCGGGATTGCAGTATCCAGAGAAGGAACCAAAAGCTGCTGGAAGAAGCACCCTCCCCTGCATTGACCCCAGAGTTGCGTAAAGCTATGGGTGATGCAGCAGTTGCCGCAGCAGCATCGATTGGTTACATTGGTGTTGGAACCGTTGAATTCCTTTTGGATGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTCGTATTCAGGTTGAGCATCCTGTGACAGAGATGATTTCCTCTGTTGACTTGATTGAAGAACAAATTCGTGTAGCTATGGGAGAAAAACTTCGATACACACAGGATGAGATAGTGCTCAGAGGACATTCAATTGAATGTCGTATCAATGCAGAAGATGCTTTTAAAGGATTCCGACCTGGGCCAGGAAGAATAACTGCCTACTTACCATCTGGAGGTCCCTTTGTTAGAATGGATAGCCATGTTTATCCTGATTATGTGGTTCCTCCAAACTATGACTCCCTTCTTGGAAAGCTTATTGTTTGGGCACCAACAAGAGAAAAGGCAATTGAACGAATGAAGAGGGCTCTTGATGACACTATTATAACAGGAGTTCCCACAACCATTGAATATCATAAACTTATCCTTGATATAGAGGATTTCAAAAATGGCAAGGTTGATACTGCTTTTATTCCAAAGCACGAAGAGGAGCTCGCAGCGCCCCAACATTTGGTGCCTGCAGCCCCTGCCAAAGAACTAGCTGGTGCATCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

534

Amino Acids

58.34

Weight (kDa)

6.56

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1430
AarI CACCTGC 1 cut(s) 563
Acc36I ACCTGC 1 cut(s) 563
AccB1I GGYRCC 2 cut(s) 1372, 1558
AccB7I CCANNNNNTGG 3 cut(s) 44, 265, 1555
AccBSI CCGCTC 1 cut(s) 874
AciI CCGC 2 cut(s) 874, 978
AclWI GGATC 1 cut(s) 67
AcsI RAATTY 4 cut(s) 207, 824, 1019, 1131
AcuI CTGAAG 1 cut(s) 738
AfaI GTAC 4 cut(s) 374, 421, 588, 800
AfiI CCNNNNNNNGG 7 cut(s) 44, 265, 440, 532, 1250, 1280, 1555
AflIII ACRYGT 1 cut(s) 191
AjiI CACGTC 1 cut(s) 692
AjnI CCWGG 6 cut(s) 43, 161, 433, 589, 1243, 1249
Alw21I GWGCWC 6 cut(s) 136, 145, 181, 617, 1185, 1539
Alw26I GTCTC 1 cut(s) 549
AlwI GGATC 1 cut(s) 67
AlwNI CAGNNNCTG 1 cut(s) 1571
Ama87I CYCGRG 1 cut(s) 135
AoxI GGCC 2 cut(s) 506, 1247
ApoI RAATTY 4 cut(s) 207, 824, 1019, 1131
Asp700I GAANNNNTTC 3 cut(s) 207, 1131, 1155
AspLEI GCGC 1 cut(s) 1546
AspS9I GGNCC 3 cut(s) 506, 1247, 1283
AsuHPI GGTGA 3 cut(s) 362, 548, 974
AsuII TTCGAA 1 cut(s) 250
AvaI CYCGRG 1 cut(s) 135
AvaII GGWCC 1 cut(s) 1283
BaeGI GKGCMC 1 cut(s) 1375
BanI GGYRCC 2 cut(s) 1372, 1558
BanII GRGCYC 4 cut(s) 136, 181, 1414, 1539
BbsI GAAGAC 1 cut(s) 572
Bbv12I GWGCWC 6 cut(s) 136, 145, 181, 617, 1185, 1539
BccI CCATC 3 cut(s) 593, 773, 1282
BceAI ACGGC 1 cut(s) 690
BcgI CGANNNNNNTGC 4 cut(s) 116, 150, 969, 1003
BciT130I CCWGG 6 cut(s) 45, 163, 435, 591, 1245, 1251
BciVI GTATCC 2 cut(s) 431, 896
BclI TGATCA 1 cut(s) 663
BcoDI GTCTC 1 cut(s) 549
BfaI CTAG 3 cut(s) 66, 813, 1583
BfmI CTRYAG 3 cut(s) 608, 759, 1563
BfoI RGCGCY 1 cut(s) 1547
BfuAI ACCTGC 1 cut(s) 563
BfuI GTATCC 2 cut(s) 431, 896
Bme1390I CCNGG 6 cut(s) 45, 163, 435, 591, 1245, 1251
Bme18I GGWCC 1 cut(s) 1283
BmeT110I CYCGRG 1 cut(s) 135
BmgBI CACGTC 1 cut(s) 692
BmgT120I GGNCC 3 cut(s) 506, 1247, 1283
BmiI GGNNCC 7 cut(s) 899, 1012, 1042, 1285, 1329, 1374, 1560
BmrFI CCNGG 6 cut(s) 45, 163, 435, 591, 1245, 1251
BmsI GCATC 7 cut(s) 439, 470, 531, 955, 995, 1093, 1213
BpiI GAAGAC 1 cut(s) 572
BpmI CTGGAG 1 cut(s) 1299
Bpu10I CCTNAGC 1 cut(s) 510
Bpu14I TTCGAA 1 cut(s) 250
BpuEI CTTGAG 1 cut(s) 129
Bsa29I ATCGAT 1 cut(s) 989
BsaJI CCNNGG 1 cut(s) 1244
BsaXI ACNNNNNCTCC 4 cut(s) 25, 55, 1328, 1358
Bsc4I CCNNNNNNNGG 7 cut(s) 44, 265, 440, 532, 1250, 1280, 1555
BseBI CCWGG 6 cut(s) 45, 163, 435, 591, 1245, 1251
BseCI ATCGAT 1 cut(s) 989
BseDI CCNNGG 1 cut(s) 1244
BseGI GGATG 7 cut(s) 10, 430, 522, 801, 1036, 1084, 1177
BseLI CCNNNNNNNGG 7 cut(s) 44, 265, 440, 532, 1250, 1280, 1555
BseMII CTCAG 2 cut(s) 444, 1198
BseRI GAGGAG 1 cut(s) 1547
BseSI GKGCMC 1 cut(s) 1375
BsgI GTGCAG 1 cut(s) 499
BshFI GGCC 2 cut(s) 508, 1249
BshNI GGYRCC 2 cut(s) 1372, 1558
BshVI ATCGAT 1 cut(s) 989
BsiHKAI GWGCWC 6 cut(s) 136, 145, 181, 617, 1185, 1539
BsiHKCI CYCGRG 1 cut(s) 135
BslFI GGGAC 1 cut(s) 1269
BslI CCNNNNNNNGG 7 cut(s) 44, 265, 440, 532, 1250, 1280, 1555
BsmAI GTCTC 1 cut(s) 549
BsmFI GGGAC 1 cut(s) 1269
BsmI GAATGC 1 cut(s) 463
BsnI GGCC 2 cut(s) 508, 1249
BsoBI CYCGRG 1 cut(s) 135
Bsp119I TTCGAA 1 cut(s) 250
Bsp1286I GDGCHC 8 cut(s) 136, 145, 181, 617, 1185, 1375, 1414, 1539
Bsp1407I TGTACA 1 cut(s) 419
Bsp143I GATC 2 cut(s) 72, 663
BspACI CCGC 2 cut(s) 874, 978
BspANI GGCC 2 cut(s) 508, 1249
BspCNI CTCAG 2 cut(s) 445, 1197
BspDI ATCGAT 1 cut(s) 989
BspLI GGNNCC 7 cut(s) 899, 1012, 1042, 1285, 1329, 1374, 1560
BspMAI CTGCAG 2 cut(s) 763, 1567
BspMI ACCTGC 1 cut(s) 563
BspPI GGATC 1 cut(s) 67
BspT104I TTCGAA 1 cut(s) 250
BspT107I GGYRCC 2 cut(s) 1372, 1558
BsrBI CCGCTC 1 cut(s) 874
BsrGI TGTACA 1 cut(s) 419
BssECI CCNNGG 1 cut(s) 1244
BssMI GATC 2 cut(s) 72, 663
Bst2UI CCWGG 6 cut(s) 45, 163, 435, 591, 1245, 1251
Bst4CI ACNGT 3 cut(s) 443, 586, 1015
Bst6I CTCTTC 3 cut(s) 714, 1400, 1524
BstAPI GCANNNNNTGC 3 cut(s) 974, 1223, 1571
BstAUI TGTACA 1 cut(s) 419
BstBI TTCGAA 1 cut(s) 250
BstC8I GCNNGC 6 cut(s) 361, 636, 698, 744, 1539, 1563
BstDEI CTNAG 5 cut(s) 376, 453, 510, 1184, 1599
BstENI CCTNNNNNAGG 1 cut(s) 1248
BstF5I GGATG 7 cut(s) 10, 430, 522, 801, 1036, 1084, 1177
BstH2I RGCGCY 1 cut(s) 1547
BstHHI GCGC 1 cut(s) 1546
BstKTI GATC 2 cut(s) 75, 666
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 2 cut(s) 72, 663
BstNI CCWGG 6 cut(s) 45, 163, 435, 591, 1245, 1251
BstNSI RCATGY 2 cut(s) 195, 700
BstSCI CCNGG 6 cut(s) 43, 161, 433, 589, 1243, 1249
BstSFI CTRYAG 3 cut(s) 608, 759, 1563
BstSLI GKGCMC 1 cut(s) 1375
BstV2I GAAGAC 1 cut(s) 572
BstX2I RGATCY 1 cut(s) 72
BstYI RGATCY 1 cut(s) 72
Bsu15I ATCGAT 1 cut(s) 989
BsuI GTATCC 2 cut(s) 431, 896
BsuRI GGCC 2 cut(s) 508, 1249
BsuTUI ATCGAT 1 cut(s) 989
BtrI CACGTC 1 cut(s) 692
BtsCI GGATG 7 cut(s) 10, 430, 522, 801, 1036, 1084, 1177
BveI ACCTGC 1 cut(s) 563
Cac8I GCNNGC 6 cut(s) 361, 636, 698, 744, 1539, 1563
CaiI CAGNNNCTG 1 cut(s) 1571
CfoI GCGC 1 cut(s) 1546
Cfr13I GGNCC 3 cut(s) 506, 1247, 1283
ClaI ATCGAT 1 cut(s) 989
CseI GACGC 2 cut(s) 317, 689
Csp6I GTAC 4 cut(s) 373, 420, 587, 799
CspCI CAANNNNNGTGG 2 cut(s) 1432, 1467
CviAII CATG 8 cut(s) 170, 185, 192, 260, 317, 697, 1054, 1307
CviQI GTAC 4 cut(s) 373, 420, 587, 799
DdeI CTNAG 5 cut(s) 376, 453, 510, 1184, 1599
DpnI GATC 2 cut(s) 74, 665
DpnII GATC 2 cut(s) 72, 663
DraI TTTAAA 1 cut(s) 1231
Eam1104I CTCTTC 3 cut(s) 714, 1400, 1524
EarI CTCTTC 3 cut(s) 714, 1400, 1524
Ecl136II GAGCTC 3 cut(s) 134, 179, 1537
Eco24I GRGCYC 4 cut(s) 136, 181, 1414, 1539
Eco47I GGWCC 1 cut(s) 1283
Eco53kI GAGCTC 3 cut(s) 134, 179, 1537
Eco57I CTGAAG 1 cut(s) 738
Eco88I CYCGRG 1 cut(s) 135
EcoICRI GAGCTC 3 cut(s) 134, 179, 1537
EcoNI CCTNNNNNAGG 1 cut(s) 1248
EcoO109I RGGNCCY 1 cut(s) 1283
EcoRI GAATTC 1 cut(s) 1019
EcoRII CCWGG 6 cut(s) 43, 161, 433, 589, 1243, 1249
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 4 cut(s) 136, 181, 1414, 1539
FaeI CATG 8 cut(s) 173, 188, 195, 263, 320, 700, 1057, 1310
FaqI GGGAC 1 cut(s) 1269
FatI CATG 8 cut(s) 169, 184, 191, 259, 316, 696, 1053, 1306
FauI CCCGC 1 cut(s) 867
FbaI TGATCA 1 cut(s) 663
FokI GGATG 7 cut(s) 17, 417, 509, 788, 1043, 1071, 1184
FriOI GRGCYC 4 cut(s) 136, 181, 1414, 1539
FspBI CTAG 3 cut(s) 66, 813, 1583
GlaI GCGC 1 cut(s) 1545
GsuI CTGGAG 1 cut(s) 1299
HaeII RGCGCY 1 cut(s) 1547
HaeIII GGCC 2 cut(s) 508, 1249
HgaI GACGC 2 cut(s) 317, 689
HhaI GCGC 1 cut(s) 1546
Hin1II CATG 8 cut(s) 173, 188, 195, 263, 320, 700, 1057, 1310
Hin6I GCGC 1 cut(s) 1544
HinP1I GCGC 1 cut(s) 1544
HincII GTYRAC 2 cut(s) 115, 1114
HindII GTYRAC 2 cut(s) 115, 1114
HindIII AAGCTT 1 cut(s) 1358
HinfI GANTC 5 cut(s) 335, 492, 627, 1236, 1343
HphI GGTGA 3 cut(s) 362, 548, 974
Hpy166II GTNNAC 5 cut(s) 115, 289, 787, 862, 1114
Hpy188I TCNGA 3 cut(s) 414, 1187, 1241
Hpy188III TCNNGA 5 cut(s) 76, 889, 1278, 1316, 1415
Hpy8I GTNNAC 5 cut(s) 115, 289, 787, 862, 1114
HpyAV CCTTC 3 cut(s) 617, 888, 1358
HpyCH4III ACNGT 3 cut(s) 443, 586, 1015
HpyCH4IV ACGT 1 cut(s) 691
HpyF3I CTNAG 5 cut(s) 376, 453, 510, 1184, 1599
HpySE526I ACGT 1 cut(s) 691
Hsp92II CATG 8 cut(s) 173, 188, 195, 263, 320, 700, 1057, 1310
HspAI GCGC 1 cut(s) 1544
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 2 cut(s) 72, 663
LmnI GCTCC 2 cut(s) 176, 1534
LweI GCATC 7 cut(s) 439, 470, 531, 955, 995, 1093, 1213
MaeI CTAG 3 cut(s) 66, 813, 1583
MaeII ACGT 1 cut(s) 691
MaeIII GTNAC 5 cut(s) 31, 148, 735, 995, 1090
MalI GATC 2 cut(s) 74, 665
MbiI CCGCTC 1 cut(s) 874
MboI GATC 2 cut(s) 72, 663
MboII GAAGA 8 cut(s) 577, 731, 926, 1136, 1232, 1266, 1417, 1541
MfeI CAATTG 2 cut(s) 1197, 1392
MflI RGATCY 1 cut(s) 72
MhlI GDGCHC 8 cut(s) 136, 145, 181, 617, 1185, 1375, 1414, 1539
MluCI AATT 8 cut(s) 207, 231, 323, 824, 1019, 1131, 1197, 1392
MlyI GAGTC 1 cut(s) 1337
MmeI TCCRAC 5 cut(s) 62, 392, 605, 988, 1264
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 3 cut(s) 207, 1131, 1155
MseI TTAA 2 cut(s) 122, 1230
MslI CAYNNNNRTG 1 cut(s) 1058
MspA1I CMGCKG 1 cut(s) 764
MspR9I CCNGG 6 cut(s) 45, 163, 435, 591, 1245, 1251
MunI CAATTG 2 cut(s) 1197, 1392
Mva1269I GAATGC 1 cut(s) 463
MvaI CCWGG 6 cut(s) 45, 163, 435, 591, 1245, 1251
NdeII GATC 2 cut(s) 72, 663
NlaIII CATG 8 cut(s) 173, 188, 195, 263, 320, 700, 1057, 1310
NlaIV GGNNCC 7 cut(s) 899, 1012, 1042, 1285, 1329, 1374, 1560
NmuCI GTSAC 2 cut(s) 31, 1090
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 2 cut(s) 195, 700
NspV TTCGAA 1 cut(s) 250
PaeI GCATGC 1 cut(s) 700
PaqCI CACCTGC 1 cut(s) 563
PciI ACATGT 1 cut(s) 191
PcsI WCGNNNNNNNCGW 2 cut(s) 247, 697
PctI GAATGC 1 cut(s) 463
PdmI GAANNNNTTC 3 cut(s) 207, 1131, 1155
PfeI GAWTC 4 cut(s) 335, 492, 627, 1236
PflMI CCANNNNNTGG 3 cut(s) 44, 265, 1555
PfoI TCCNGGA 2 cut(s) 161, 433
PleI GAGTC 1 cut(s) 1337
PpsI GAGTC 1 cut(s) 1337
PpuMI RGGWCCY 1 cut(s) 1283
PscI ACATGT 1 cut(s) 191
PsiI TTATAA 1 cut(s) 1430
Psp124BI GAGCTC 3 cut(s) 136, 181, 1539
Psp5II RGGWCCY 1 cut(s) 1283
Psp6I CCWGG 6 cut(s) 43, 161, 433, 589, 1243, 1249
PspGI CCWGG 6 cut(s) 43, 161, 433, 589, 1243, 1249
PspN4I GGNNCC 7 cut(s) 899, 1012, 1042, 1285, 1329, 1374, 1560
PspPI GGNCC 3 cut(s) 506, 1247, 1283
PspPPI RGGWCCY 1 cut(s) 1283
PstI CTGCAG 2 cut(s) 763, 1567
PstNI CAGNNNCTG 1 cut(s) 1571
PsuI RGATCY 1 cut(s) 72
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 4 cut(s) 374, 421, 588, 800
RsaNI GTAC 4 cut(s) 373, 420, 587, 799
RseI CAYNNNNRTG 1 cut(s) 1058
SacI GAGCTC 3 cut(s) 136, 181, 1539
SaqAI TTAA 2 cut(s) 122, 1230
Sau3AI GATC 2 cut(s) 72, 663
Sau96I GGNCC 3 cut(s) 506, 1247, 1283
SchI GAGTC 1 cut(s) 1337
ScrFI CCNGG 6 cut(s) 45, 163, 435, 591, 1245, 1251
SduI GDGCHC 8 cut(s) 136, 145, 181, 617, 1185, 1375, 1414, 1539
SfaNI GCATC 7 cut(s) 439, 470, 531, 955, 995, 1093, 1213
SfcI CTRYAG 3 cut(s) 608, 759, 1563
SfuI TTCGAA 1 cut(s) 250
SinI GGWCC 1 cut(s) 1283
SmiMI CAYNNNNRTG 1 cut(s) 1058
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
SphI GCATGC 1 cut(s) 700
Sse9I AATT 8 cut(s) 207, 231, 323, 824, 1019, 1131, 1197, 1392
SsiI CCGC 2 cut(s) 874, 978
SspMI CTAG 3 cut(s) 66, 813, 1583
SstI GAGCTC 3 cut(s) 136, 181, 1539
StyD4I CCNGG 6 cut(s) 43, 161, 433, 589, 1243, 1249
TaaI ACNGT 3 cut(s) 443, 586, 1015
TaiI ACGT 1 cut(s) 694
TaqI TCGA 3 cut(s) 250, 989, 1159
TasI AATT 8 cut(s) 207, 231, 323, 824, 1019, 1131, 1197, 1392
TatI WGTACW 2 cut(s) 419, 798
TauI GCSGC 1 cut(s) 980
TfiI GAWTC 4 cut(s) 335, 492, 627, 1236
Tru1I TTAA 2 cut(s) 122, 1230
Tru9I TTAA 2 cut(s) 122, 1230
TseFI GTSAC 2 cut(s) 31, 1090
Tsp45I GTSAC 2 cut(s) 31, 1090
TspDTI ATGAA 8 cut(s) 276, 305, 348, 578, 1042, 1047, 1076, 1418
TspGWI ACGGA 2 cut(s) 504, 515
Van91I CCANNNNNTGG 3 cut(s) 44, 265, 1555
VpaK11BI GGWCC 1 cut(s) 1283
XagI CCTNNNNNAGG 1 cut(s) 1248
XapI RAATTY 4 cut(s) 207, 824, 1019, 1131
XceI RCATGY 2 cut(s) 195, 700
XmnI GAANNNNTTC 3 cut(s) 207, 1131, 1155
XspI CTAG 3 cut(s) 66, 813, 1583
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.