RchiOBHm_Chr4g0397731

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004128
Physical Location & Seq
Reverse (-)
14133933 .. 14140305
6373 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36999

Sequence Viewer

Length: 1602 bp
ATGGACGCCACCATGCCCATTTGCAAGACTGTTACTCCAACTCCGCGCTTATTTTTTGGGAAAACTGGAGAGATCAGGAGTTCACAATGTAGCTTTATGGTGGGAAATAAAGTTAGCTTTTTGAGGCAGAGAGCTCAGGGCGCTCAGATTAGTTATAGATCAAGGAAGCATGCCGGAGCTCTCCATGCTACGTGTCTTGCTGATAAAATCCTGGTGGCAAATAGAGGAGAAATTGCTGTTCGTGTTATTCGAACGGCGCATGAGATGGGCATTCCCTGTGTGGCTGTTTACTCAACAATAGACAAGGATGCACTTCATGTCAAATTGGCTGATGAATCAGTTTGTATTGGTGAAGCAGCAAGCAGTCAATCGTACCTAGTGGTTCCAAATGTTTTATCTGCTGCCATCAGTCGTAAATGTACAATGCTTCATCCTGGATATGGTTTCCTGTCTGAGAATGCATCTTTTGTTGAAATGTGCAAAGAACATGGAATCAACTTTATTGGTCCTAAGCCCGAAAGTATCCGTATTATGGGTGATAAAGCGACTGCCAGAGAAACAATGAAGCAAGCAGGTGTTCCAACTGTACCAGGAAGTGATGGATTACTACAGAGCACAGAGGAAGGAATCAAGCTTGCCGATGAGATTGGTTATCCTGTTATGATCAAGGCAACAGCAGGTGGTGGAGGACGTGGTATGCGTCTTGCTAAAGAGCCTGAAGAGTTTGTAAAGTTGCTACAGGCAGCAAAGAGCGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTATGTCGTGAATCCTAGACACATTGAATTTCAGGTTCTTGCAGATAAGTATGGTAATGTTGTTCACTTTGGAGAGCGTGATTGTAGCATCCAGAGAAGGAACCAAAAGCTGTTAGAAGAAGCACCCTCTCCTGCATTGACCCCAGAATTGCGAAAAGCCATGGGTGATGCAGCAGTTGCTGCCGCAGCATCTATTGGTTATATTGGTGTTGGAACCATTGAGTTCCTTTTGGACGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTAGGATTCAGGTTGAACATCCTGTGACAGAAATGATTTCCTCTGTTGACTTGATTGAAGAACAAATTCGTGTTGCTATGGGAGAAAAACTCCGATACACACAGGATGATATCGTGCTCAGAGGGCATTCAATTGAATGTCGTATCAATGCAGAAGATGCTTTTAAAGGATTCCGACCTGGGCCAGGTAGAATAACAGCTTACTTACCCGCCGGAGGTCCATTTGTTAGAATGGATAGTCATGTTTATCCTGATTATGTGGTTCCTCCAAACTATGATTCCCTTCTTGGAAAGCTTATTGTTTGGGCACCAACAAGAGAAAAGGCAATTGATCGGATGAAAAGAGCTCTTAACGACACTATTATAATAGGGGTTCCTACAACTATTGAATATCATAAGCTCATCCTGGAGATAGACGATTTCAAAAATGGGAAGGTTGATACTGCTTTTATTCCCAAGCATGAAGAGGAGCTAGCAGCGCCCCAACAAGTGTTGCCTGCATTCCCACCCAAAGAACTAGCTAGTGCAAGTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

533

Amino Acids

58.34

Weight (kDa)

6.53

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 68 - 175 1.1e-41 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 180 - 387 5.7e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 189 - 357 3e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 207 - 356 6.2e-07 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 401 - 507 1.7e-37 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1430
AarI CACCTGC 2 cut(s) 563, 668
Acc36I ACCTGC 2 cut(s) 563, 668
AccB1I GGYRCC 1 cut(s) 1372
AccII CGCG 1 cut(s) 46
AciI CCGC 3 cut(s) 44, 981, 1275
AcsI RAATTY 2 cut(s) 824, 1131
AcuI CTGAAG 1 cut(s) 738
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 4 cut(s) 374, 421, 588, 1597
AfiI CCNNNNNNNGG 4 cut(s) 440, 532, 1250, 1280
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 8 cut(s) 473, 824, 1082, 1124, 1197, 1202, 1454, 1489
AjiI CACGTC 1 cut(s) 692
AjnI CCWGG 6 cut(s) 210, 433, 589, 1243, 1249, 1470
AjuI GAANNNNNNNTTGG 2 cut(s) 1327, 1359
Alw21I GWGCWC 5 cut(s) 136, 181, 617, 1185, 1414
AlwNI CAGNNNCTG 1 cut(s) 977
AoxI GGCC 1 cut(s) 1247
ApoI RAATTY 2 cut(s) 824, 1131
Asp700I GAANNNNTTC 1 cut(s) 1131
AspLEI GCGC 4 cut(s) 48, 143, 259, 1546
AspS9I GGNCC 3 cut(s) 506, 1247, 1283
AsuHPI GGTGA 3 cut(s) 362, 548, 974
AsuII TTCGAA 1 cut(s) 250
AsuNHI GCTAGC 1 cut(s) 1537
AvaII GGWCC 2 cut(s) 506, 1283
BaeGI GKGCMC 1 cut(s) 1375
BanI GGYRCC 1 cut(s) 1372
BanII GRGCYC 3 cut(s) 136, 181, 1414
Bbv12I GWGCWC 5 cut(s) 136, 181, 617, 1185, 1414
BccI CCATC 4 cut(s) 259, 413, 593, 773
BceAI ACGGC 1 cut(s) 270
BciT130I CCWGG 6 cut(s) 212, 435, 591, 1245, 1251, 1472
BciVI GTATCC 1 cut(s) 533
BclI TGATCA 1 cut(s) 663
BfaI CTAG 6 cut(s) 377, 813, 1068, 1538, 1583, 1587
BfmI CTRYAG 3 cut(s) 608, 737, 759
BfoI RGCGCY 2 cut(s) 144, 1547
BfuAI ACCTGC 2 cut(s) 563, 668
BfuI GTATCC 1 cut(s) 533
Bme1390I CCNGG 6 cut(s) 212, 435, 591, 1245, 1251, 1472
Bme18I GGWCC 2 cut(s) 506, 1283
BmgBI CACGTC 1 cut(s) 692
BmgT120I GGNCC 3 cut(s) 506, 1247, 1283
BmiI GGNNCC 7 cut(s) 384, 899, 1012, 1042, 1329, 1374, 1440
BmrFI CCNGG 6 cut(s) 212, 435, 591, 1245, 1251, 1472
BmsI GCATC 6 cut(s) 298, 470, 894, 955, 995, 1213
BmtI GCTAGC 1 cut(s) 1541
BplI GAGNNNNNCTC 2 cut(s) 1140, 1172
BpmI CTGGAG 2 cut(s) 87, 1493
Bpu10I CCTNAGC 2 cut(s) 135, 510
Bpu14I TTCGAA 1 cut(s) 250
BsaAI YACGTR 2 cut(s) 192, 1599
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 2 cut(s) 957, 1244
BsaXI ACNNNNNCTCC 4 cut(s) 19, 25, 49, 55
Bsc4I CCNNNNNNNGG 4 cut(s) 440, 532, 1250, 1280
Bse1I ACTGG 1 cut(s) 70
BseBI CCWGG 6 cut(s) 212, 435, 591, 1245, 1251, 1472
BseDI CCNNGG 2 cut(s) 957, 1244
BseGI GGATG 7 cut(s) 313, 430, 885, 1084, 1177, 1407, 1467
BseLI CCNNNNNNNGG 4 cut(s) 440, 532, 1250, 1280
BseMII CTCAG 4 cut(s) 149, 158, 444, 1198
BseNI ACTGG 1 cut(s) 70
BseRI GAGGAG 2 cut(s) 240, 1547
BseSI GKGCMC 1 cut(s) 1375
Bsh1236I CGCG 1 cut(s) 46
BshFI GGCC 1 cut(s) 1249
BshNI GGYRCC 1 cut(s) 1372
BsiHKAI GWGCWC 5 cut(s) 136, 181, 617, 1185, 1414
BsiSI CCGG 2 cut(s) 174, 1278
BslI CCNNNNNNNGG 4 cut(s) 440, 532, 1250, 1280
BsmI GAATGC 4 cut(s) 270, 463, 1192, 1565
BsnI GGCC 1 cut(s) 1249
Bsp119I TTCGAA 1 cut(s) 250
Bsp1286I GDGCHC 6 cut(s) 136, 181, 617, 1185, 1375, 1414
Bsp1407I TGTACA 1 cut(s) 419
Bsp143I GATC 4 cut(s) 72, 158, 663, 1396
Bsp19I CCATGG 1 cut(s) 957
BspACI CCGC 3 cut(s) 44, 981, 1275
BspANI GGCC 1 cut(s) 1249
BspCNI CTCAG 4 cut(s) 148, 157, 445, 1197
BspFNI CGCG 1 cut(s) 46
BspLI GGNNCC 7 cut(s) 384, 899, 1012, 1042, 1329, 1374, 1440
BspMAI CTGCAG 1 cut(s) 763
BspMI ACCTGC 2 cut(s) 563, 668
BspOI GCTAGC 1 cut(s) 1541
BspT104I TTCGAA 1 cut(s) 250
BspT107I GGYRCC 1 cut(s) 1372
BsrGI TGTACA 1 cut(s) 419
BsrI ACTGG 1 cut(s) 70
BssECI CCNNGG 2 cut(s) 957, 1244
BssMI GATC 4 cut(s) 72, 158, 663, 1396
BssNI GRCGYC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 957
Bst2UI CCWGG 6 cut(s) 212, 435, 591, 1245, 1251, 1472
Bst4CI ACNGT 2 cut(s) 31, 586
Bst6I CTCTTC 2 cut(s) 714, 1524
BstACI GRCGYC 1 cut(s) 6
BstAPI GCANNNNNTGC 3 cut(s) 974, 977, 1223
BstAUI TGTACA 1 cut(s) 419
BstBAI YACGTR 2 cut(s) 192, 1599
BstBI TTCGAA 1 cut(s) 250
BstC8I GCNNGC 6 cut(s) 171, 361, 570, 636, 1539, 1563
BstDEI CTNAG 5 cut(s) 135, 144, 453, 510, 1184
BstDSI CCRYGG 1 cut(s) 957
BstENI CCTNNNNNAGG 1 cut(s) 1248
BstF5I GGATG 7 cut(s) 313, 430, 885, 1084, 1177, 1407, 1467
BstFNI CGCG 1 cut(s) 46
BstH2I RGCGCY 2 cut(s) 144, 1547
BstHHI GCGC 4 cut(s) 48, 143, 259, 1546
BstKTI GATC 4 cut(s) 75, 161, 666, 1399
BstMBI GATC 4 cut(s) 72, 158, 663, 1396
BstMWI GCNNNNNNNGC 9 cut(s) 140, 185, 764, 974, 977, 983, 1189, 1223, 1543
BstNI CCWGG 6 cut(s) 212, 435, 591, 1245, 1251, 1472
BstNSI RCATGY 1 cut(s) 173
BstSCI CCNGG 6 cut(s) 210, 433, 589, 1243, 1249, 1470
BstSFI CTRYAG 3 cut(s) 608, 737, 759
BstSLI GKGCMC 1 cut(s) 1375
BstSNI TACGTA 1 cut(s) 1599
BstUI CGCG 1 cut(s) 46
BsuI GTATCC 1 cut(s) 533
BsuRI GGCC 1 cut(s) 1249
BtgI CCRYGG 1 cut(s) 957
BtrI CACGTC 1 cut(s) 692
BtsCI GGATG 7 cut(s) 313, 430, 885, 1084, 1177, 1407, 1467
BveI ACCTGC 2 cut(s) 563, 668
Cac8I GCNNGC 6 cut(s) 171, 361, 570, 636, 1539, 1563
CaiI CAGNNNCTG 1 cut(s) 977
CfoI GCGC 4 cut(s) 48, 143, 259, 1546
Cfr13I GGNCC 3 cut(s) 506, 1247, 1283
CseI GACGC 2 cut(s) 14, 689
Csp6I GTAC 4 cut(s) 373, 420, 587, 1596
CviQI GTAC 4 cut(s) 373, 420, 587, 1596
DdeI CTNAG 5 cut(s) 135, 144, 453, 510, 1184
DpnI GATC 4 cut(s) 74, 160, 665, 1398
DpnII GATC 4 cut(s) 72, 158, 663, 1396
DraI TTTAAA 1 cut(s) 1231
Eam1104I CTCTTC 2 cut(s) 714, 1524
EarI CTCTTC 2 cut(s) 714, 1524
Ecl136II GAGCTC 3 cut(s) 134, 179, 1412
Eco105I TACGTA 1 cut(s) 1599
Eco130I CCWWGG 1 cut(s) 957
Eco24I GRGCYC 3 cut(s) 136, 181, 1414
Eco32I GATATC 1 cut(s) 1177
Eco47I GGWCC 2 cut(s) 506, 1283
Eco53kI GAGCTC 3 cut(s) 134, 179, 1412
Eco57I CTGAAG 1 cut(s) 738
EcoICRI GAGCTC 3 cut(s) 134, 179, 1412
EcoNI CCTNNNNNAGG 1 cut(s) 1248
EcoRII CCWGG 6 cut(s) 210, 433, 589, 1243, 1249, 1470
EcoRV GATATC 1 cut(s) 1177
EcoT14I CCWWGG 1 cut(s) 957
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 3 cut(s) 136, 181, 1414
ErhI CCWWGG 1 cut(s) 957
FauI CCCGC 1 cut(s) 1282
FbaI TGATCA 1 cut(s) 663
FokI GGATG 7 cut(s) 320, 417, 872, 1071, 1184, 1414, 1454
FriOI GRGCYC 3 cut(s) 136, 181, 1414
FspBI CTAG 6 cut(s) 377, 813, 1068, 1538, 1583, 1587
GlaI GCGC 4 cut(s) 47, 142, 258, 1545
GsuI CTGGAG 2 cut(s) 87, 1493
HaeII RGCGCY 2 cut(s) 144, 1547
HaeIII GGCC 1 cut(s) 1249
HapII CCGG 2 cut(s) 174, 1278
HgaI GACGC 2 cut(s) 14, 689
HhaI GCGC 4 cut(s) 48, 143, 259, 1546
Hin1I GRCGYC 1 cut(s) 6
Hin6I GCGC 4 cut(s) 46, 141, 257, 1544
HinP1I GCGC 4 cut(s) 46, 141, 257, 1544
HincII GTYRAC 1 cut(s) 1114
HindII GTYRAC 1 cut(s) 1114
HindIII AAGCTT 2 cut(s) 632, 1358
HinfI GANTC 7 cut(s) 335, 492, 627, 808, 1072, 1236, 1343
HpaII CCGG 2 cut(s) 174, 1278
HphI GGTGA 3 cut(s) 362, 548, 974
Hpy166II GTNNAC 4 cut(s) 83, 289, 862, 1114
Hpy188I TCNGA 6 cut(s) 147, 454, 1160, 1187, 1241, 1401
Hpy188III TCNNGA 4 cut(s) 76, 805, 889, 1316
Hpy8I GTNNAC 4 cut(s) 83, 289, 862, 1114
HpyAV CCTTC 4 cut(s) 617, 888, 1358, 1492
HpyCH4III ACNGT 2 cut(s) 31, 586
HpyCH4IV ACGT 3 cut(s) 191, 691, 1598
HpyF10VI GCNNNNNNNGC 9 cut(s) 140, 185, 764, 974, 977, 983, 1189, 1223, 1543
HpyF3I CTNAG 5 cut(s) 135, 144, 453, 510, 1184
HpySE526I ACGT 3 cut(s) 191, 691, 1598
Hsp92I GRCGYC 1 cut(s) 6
HspAI GCGC 4 cut(s) 46, 141, 257, 1544
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 4 cut(s) 72, 158, 663, 1396
LmnI GCTCC 2 cut(s) 176, 1534
LweI GCATC 6 cut(s) 298, 470, 894, 955, 995, 1213
MaeI CTAG 6 cut(s) 377, 813, 1068, 1538, 1583, 1587
MaeII ACGT 3 cut(s) 191, 691, 1598
MaeIII GTNAC 2 cut(s) 31, 1090
MalI GATC 4 cut(s) 74, 160, 665, 1398
MboI GATC 4 cut(s) 72, 158, 663, 1396
MboII GAAGA 5 cut(s) 731, 926, 1136, 1232, 1541
MfeI CAATTG 2 cut(s) 1197, 1392
MhlI GDGCHC 6 cut(s) 136, 181, 617, 1185, 1375, 1414
MluCI AATT 7 cut(s) 231, 323, 824, 944, 1131, 1197, 1392
MmeI TCCRAC 4 cut(s) 62, 605, 988, 1264
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 1131
MseI TTAA 2 cut(s) 1230, 1416
MslI CAYNNNNRTG 1 cut(s) 1058
MspA1I CMGCKG 1 cut(s) 764
MspI CCGG 2 cut(s) 174, 1278
MspR9I CCNGG 6 cut(s) 212, 435, 591, 1245, 1251, 1472
MunI CAATTG 2 cut(s) 1197, 1392
Mva1269I GAATGC 4 cut(s) 270, 463, 1192, 1565
MvaI CCWGG 6 cut(s) 212, 435, 591, 1245, 1251, 1472
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 9 cut(s) 140, 185, 764, 974, 977, 983, 1189, 1223, 1543
NcoI CCATGG 1 cut(s) 957
NdeII GATC 4 cut(s) 72, 158, 663, 1396
NheI GCTAGC 1 cut(s) 1537
NlaIV GGNNCC 7 cut(s) 384, 899, 1012, 1042, 1329, 1374, 1440
NmuCI GTSAC 1 cut(s) 1090
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 1 cut(s) 173
NspV TTCGAA 1 cut(s) 250
PaeI GCATGC 1 cut(s) 173
PaqCI CACCTGC 2 cut(s) 563, 668
PcsI WCGNNNNNNNCGW 2 cut(s) 247, 697
PctI GAATGC 4 cut(s) 270, 463, 1192, 1565
PdmI GAANNNNTTC 1 cut(s) 1131
PfeI GAWTC 7 cut(s) 335, 492, 627, 808, 1072, 1236, 1343
PfoI TCCNGGA 2 cut(s) 433, 1470
Ppu21I YACGTR 2 cut(s) 192, 1599
PsiI TTATAA 1 cut(s) 1430
Psp124BI GAGCTC 3 cut(s) 136, 181, 1414
Psp6I CCWGG 6 cut(s) 210, 433, 589, 1243, 1249, 1470
PspGI CCWGG 6 cut(s) 210, 433, 589, 1243, 1249, 1470
PspN4I GGNNCC 7 cut(s) 384, 899, 1012, 1042, 1329, 1374, 1440
PspPI GGNCC 3 cut(s) 506, 1247, 1283
PstI CTGCAG 1 cut(s) 763
PstNI CAGNNNCTG 1 cut(s) 977
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 4 cut(s) 374, 421, 588, 1597
RsaNI GTAC 4 cut(s) 373, 420, 587, 1596
RseI CAYNNNNRTG 1 cut(s) 1058
SacI GAGCTC 3 cut(s) 136, 181, 1414
SaqAI TTAA 2 cut(s) 1230, 1416
Sau3AI GATC 4 cut(s) 72, 158, 663, 1396
Sau96I GGNCC 3 cut(s) 506, 1247, 1283
ScrFI CCNGG 6 cut(s) 212, 435, 591, 1245, 1251, 1472
SduI GDGCHC 6 cut(s) 136, 181, 617, 1185, 1375, 1414
SfaNI GCATC 6 cut(s) 298, 470, 894, 955, 995, 1213
SfcI CTRYAG 3 cut(s) 608, 737, 759
SfuI TTCGAA 1 cut(s) 250
SinI GGWCC 2 cut(s) 506, 1283
SmiMI CAYNNNNRTG 1 cut(s) 1058
SnaBI TACGTA 1 cut(s) 1599
SphI GCATGC 1 cut(s) 173
Sse9I AATT 7 cut(s) 231, 323, 824, 944, 1131, 1197, 1392
SsiI CCGC 3 cut(s) 44, 981, 1275
SspMI CTAG 6 cut(s) 377, 813, 1068, 1538, 1583, 1587
SstI GAGCTC 3 cut(s) 136, 181, 1414
StyD4I CCNGG 6 cut(s) 210, 433, 589, 1243, 1249, 1470
StyI CCWWGG 1 cut(s) 957
TaaI ACNGT 2 cut(s) 31, 586
TaiI ACGT 3 cut(s) 194, 694, 1601
TaqI TCGA 1 cut(s) 250
TasI AATT 7 cut(s) 231, 323, 824, 944, 1131, 1197, 1392
TatI WGTACW 1 cut(s) 419
TauI GCSGC 1 cut(s) 983
TfiI GAWTC 7 cut(s) 335, 492, 627, 808, 1072, 1236, 1343
Tru1I TTAA 2 cut(s) 1230, 1416
Tru9I TTAA 2 cut(s) 1230, 1416
TseFI GTSAC 1 cut(s) 1090
Tsp45I GTSAC 1 cut(s) 1090
TspDTI ATGAA 8 cut(s) 305, 348, 419, 578, 1042, 1076, 1418, 1542
TspGWI ACGGA 1 cut(s) 515
VpaK11BI GGWCC 2 cut(s) 506, 1283
XagI CCTNNNNNAGG 1 cut(s) 1248
XapI RAATTY 2 cut(s) 824, 1131
XceI RCATGY 1 cut(s) 173
XmnI GAANNNNTTC 1 cut(s) 1131
XspI CTAG 6 cut(s) 377, 813, 1068, 1538, 1583, 1587
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.