RLG00000009418

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004128
Physical Location & Seq
Forward (+)
52575548 .. 52583305
7758 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009418

Sequence Viewer

Length: 1710 bp
ATGGTGGGAAATAAAATTAGCTTTTTAAGGCAGAGAGCTCAGGGCGCTCAGATTAGTTATAGATCAAGGAAGCATGCGGGAGCTCTCCATGCTACGTGTCTTGCTGATAAAATCCTGGTGGCAAATAGAGGAGAAATTGCTGTTCGTGTTATTCGAACGGCGCATGAGATGGGCATACCCTGTGTGGCTGTTTACTCAACAATAGACAAGGATGCACTTCATGTCAAATTGGCTGATGAATCAGTTTGTATTGGTGAAGCAGCAAGCAGTCAATCGTACCTAGTGGTTCCAAATGTTTTATCTGCTGCCATCAGTCGTAAATGTACAATGCTTCATCCTGGATATGGTTTCCTGTCTGAGAATGCATCTTTTGTTGAAATGTGCAGAGAACATGGAATCAACTTTATTGGTCCTAAGCCTGAAAGTATCCGTATTATGGGTGATAAAGCGACTGCCAGAGAAACAATGAAGCAAGCAGGTGTTCCAACTGTACCAGGAAGTGATGGATTACTACAGAGCACAGAGGAAGGAATCAAGCTTGCCGATGAGATTGGTTATCCTGTTATGATCAAGGCAACAGCAGGTGGTGGAGGACGTGGTATGCGTCTTGCTAAAGAGCCTGAAGAGTTTGTAAAGTTGCTACAGGCAGCAAAGAGCGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTATGTCGTAAATCCTAGACACATTGAATTTCAGGTTCTTGCAGATCAGTATGGTAATGTTGTTCACTTTGGAGAGCGTGATTGTAGCATCCAGAGAAGGAACCAAAAGTTGTTAGAAGAAGCACCCTCTCCTGCATTGACCCCAGAATTGCGAAAAGCCATGGGTGATGCAGCAGTTGCTGCCGCAGCATCTATTGGTTATATTGGTGTTGGAACCATTGAGTTCCTTTTGGACGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTAGGATTCAGGTTGAACATCCTGTGACAGAAATGATTTCCTCTGTTGACTTGATTGAAGAACAAATTCGTGTTGCTATGGGAGAAAAACTCCGATACACACAGGATGATATCGTGCTCAGAGGGCATTCAATTGAATGTCGTATCAATGCAGAAGATGCTTTTAAAGGATTCCGACCTGGGCCAGGTAGAATAACAGCTTACTTACCCGCTGGAGGTCCATTTGTTAGAATGGATAGTCATGTTTATCCTGATTATGTGGTTCCTCCAAACTATGATTCCCTTCTTGGAAAGCTTATTGTTTGGGCACCAACAAGAGAAAAGGCAATTGATCGGATGAAAAGAGCTCTTAACGACACTATTATAACAGGGGTTCCTACAACTATTGAATATCATAAGCTTATCCTGGAGATAGAGGATTTCAAAAATGGGAAGGTTGATACTGCTTTTATTCCCAAGCATGAAGAGGAGCTAGCAGCGCCCCAACAAGTGTTGCCTGCATCCCCACCCAAAGAACTAGCTAGTGCAAGCAGTGCCCCGGTGGTCCCTTTTGCTGCTCCAATGTCATCCAGGGAAATAGGCCTTCAGTTAGAGGTTGATGCACCACTGTCTCGCCACTGTGAGAGCCCAGCTGATGTTGATTGCGGCTGGAACAGCCGGCTTGAAACAGGCTGTGACTGCGGCTCTGTTGGCCTGAACCATTGCCAGTCTTGGCCTGCCTCCTCCCCAATGCCGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

570

Amino Acids

61.96

Weight (kDa)

5.76

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 36 - 143 1.5e-41 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 148 - 355 6.6e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 157 - 325 3.5e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 175 - 324 5.7e-07 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 369 - 475 2.9e-38 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1334
AarI CACCTGC 2 cut(s) 467, 572
Acc36I ACCTGC 2 cut(s) 467, 572
AccB1I GGYRCC 1 cut(s) 1276
AciI CCGC 5 cut(s) 77, 885, 1179, 1614, 1650
AcsI RAATTY 2 cut(s) 728, 1035
AcuI CTGAAG 2 cut(s) 642, 1538
AfaI GTAC 3 cut(s) 278, 325, 492
AfiI CCNNNNNNNGG 5 cut(s) 344, 436, 1154, 1184, 1702
AflIII ACRYGT 1 cut(s) 95
AgsI TTSAA 9 cut(s) 377, 728, 986, 1028, 1101, 1106, 1358, 1393, 1634
AjiI CACGTC 1 cut(s) 596
AjnI CCWGG 7 cut(s) 114, 337, 493, 1147, 1153, 1374, 1538
AjuI GAANNNNNNNTTGG 2 cut(s) 1231, 1263
Alw21I GWGCWC 5 cut(s) 40, 85, 521, 1089, 1318
Alw26I GTCTC 1 cut(s) 1584
AlwNI CAGNNNCTG 1 cut(s) 881
AoxI GGCC 4 cut(s) 1151, 1549, 1660, 1682
ApoI RAATTY 2 cut(s) 728, 1035
Asp700I GAANNNNTTC 1 cut(s) 1035
AspLEI GCGC 3 cut(s) 47, 163, 1450
AspS9I GGNCC 4 cut(s) 410, 1151, 1187, 1513
AsuC2I CCSGG 1 cut(s) 1508
AsuHPI GGTGA 3 cut(s) 266, 452, 878
AsuII TTCGAA 1 cut(s) 154
AsuNHI GCTAGC 1 cut(s) 1441
AvaII GGWCC 3 cut(s) 410, 1187, 1513
BaeGI GKGCMC 2 cut(s) 1279, 1507
BanI GGYRCC 1 cut(s) 1276
BanII GRGCYC 4 cut(s) 40, 85, 1318, 1598
Bbv12I GWGCWC 5 cut(s) 40, 85, 521, 1089, 1318
BccI CCATC 4 cut(s) 163, 317, 497, 677
BceAI ACGGC 1 cut(s) 174
BciT130I CCWGG 7 cut(s) 116, 339, 495, 1149, 1155, 1376, 1540
BciVI GTATCC 1 cut(s) 437
BclI TGATCA 1 cut(s) 567
BcnI CCSGG 1 cut(s) 1508
BcoDI GTCTC 1 cut(s) 1584
BfaI CTAG 6 cut(s) 281, 717, 972, 1442, 1487, 1491
BfmI CTRYAG 3 cut(s) 512, 641, 663
BfoI RGCGCY 2 cut(s) 48, 1451
BfuAI ACCTGC 2 cut(s) 467, 572
BfuI GTATCC 1 cut(s) 437
Bme1390I CCNGG 8 cut(s) 116, 339, 495, 1149, 1155, 1376, 1508, 1540
Bme18I GGWCC 3 cut(s) 410, 1187, 1513
BmgBI CACGTC 1 cut(s) 596
BmgT120I GGNCC 4 cut(s) 410, 1151, 1187, 1513
BmiI GGNNCC 8 cut(s) 288, 803, 916, 946, 1233, 1278, 1344, 1515
BmrFI CCNGG 8 cut(s) 116, 339, 495, 1149, 1155, 1376, 1508, 1540
BmsI GCATC 8 cut(s) 202, 374, 798, 859, 899, 1117, 1478, 1558
BmtI GCTAGC 1 cut(s) 1445
BplI GAGNNNNNCTC 2 cut(s) 1044, 1076
BpmI CTGGAG 2 cut(s) 1203, 1397
Bpu10I CCTNAGC 2 cut(s) 39, 414
Bpu14I TTCGAA 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 1508
BsaAI YACGTR 1 cut(s) 96
BsaJI CCNNGG 4 cut(s) 861, 1148, 1506, 1539
Bsc4I CCNNNNNNNGG 5 cut(s) 344, 436, 1154, 1184, 1702
Bse118I RCCGGY 1 cut(s) 1626
Bse1I ACTGG 1 cut(s) 1675
Bse3DI GCAATG 1 cut(s) 1669
BseBI CCWGG 7 cut(s) 116, 339, 495, 1149, 1155, 1376, 1540
BseDI CCNNGG 4 cut(s) 861, 1148, 1506, 1539
BseGI GGATG 8 cut(s) 217, 334, 789, 988, 1081, 1311, 1469, 1535
BseLI CCNNNNNNNGG 5 cut(s) 344, 436, 1154, 1184, 1702
BseMI GCAATG 1 cut(s) 1669
BseMII CTCAG 4 cut(s) 53, 62, 348, 1102
BseNI ACTGG 1 cut(s) 1675
BseRI GAGGAG 3 cut(s) 144, 1451, 1681
BseSI GKGCMC 2 cut(s) 1279, 1507
BseYI CCCAGC 1 cut(s) 1597
BsgI GTGCAG 1 cut(s) 403
BshFI GGCC 4 cut(s) 1153, 1551, 1662, 1684
BshNI GGYRCC 1 cut(s) 1276
BsiHKAI GWGCWC 5 cut(s) 40, 85, 521, 1089, 1318
BsiSI CCGG 3 cut(s) 1508, 1627, 1703
BslFI GGGAC 1 cut(s) 1499
BslI CCNNNNNNNGG 5 cut(s) 344, 436, 1154, 1184, 1702
BsmAI GTCTC 1 cut(s) 1584
BsmFI GGGAC 1 cut(s) 1499
BsmI GAATGC 2 cut(s) 367, 1096
BsnI GGCC 4 cut(s) 1153, 1551, 1662, 1684
Bsp119I TTCGAA 1 cut(s) 154
Bsp1286I GDGCHC 8 cut(s) 40, 85, 521, 1089, 1279, 1318, 1507, 1598
Bsp1407I TGTACA 1 cut(s) 323
Bsp143I GATC 4 cut(s) 62, 567, 745, 1300
Bsp19I CCATGG 1 cut(s) 861
BspACI CCGC 5 cut(s) 77, 885, 1179, 1614, 1650
BspANI GGCC 4 cut(s) 1153, 1551, 1662, 1684
BspCNI CTCAG 4 cut(s) 52, 61, 349, 1101
BspLI GGNNCC 8 cut(s) 288, 803, 916, 946, 1233, 1278, 1344, 1515
BspMAI CTGCAG 1 cut(s) 667
BspMI ACCTGC 2 cut(s) 467, 572
BspOI GCTAGC 1 cut(s) 1445
BspT104I TTCGAA 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 1276
BsrDI GCAATG 1 cut(s) 1669
BsrFI RCCGGY 1 cut(s) 1626
BsrGI TGTACA 1 cut(s) 323
BsrI ACTGG 1 cut(s) 1675
BssAI RCCGGY 1 cut(s) 1626
BssECI CCNNGG 4 cut(s) 861, 1148, 1506, 1539
BssMI GATC 4 cut(s) 62, 567, 745, 1300
BssT1I CCWWGG 1 cut(s) 861
Bst2UI CCWGG 7 cut(s) 116, 339, 495, 1149, 1155, 1376, 1540
Bst4CI ACNGT 3 cut(s) 490, 1578, 1589
Bst6I CTCTTC 2 cut(s) 618, 1428
BstAPI GCANNNNNTGC 4 cut(s) 878, 881, 1127, 1502
BstAUI TGTACA 1 cut(s) 323
BstBAI YACGTR 1 cut(s) 96
BstBI TTCGAA 1 cut(s) 154
BstC8I GCNNGC 9 cut(s) 75, 265, 474, 540, 1443, 1467, 1498, 1628, 1686
BstDEI CTNAG 5 cut(s) 39, 48, 357, 414, 1088
BstDSI CCRYGG 1 cut(s) 861
BstENI CCTNNNNNAGG 1 cut(s) 1152
BstF5I GGATG 8 cut(s) 217, 334, 789, 988, 1081, 1311, 1469, 1535
BstH2I RGCGCY 2 cut(s) 48, 1451
BstHHI GCGC 3 cut(s) 47, 163, 1450
BstKTI GATC 4 cut(s) 65, 570, 748, 1303
BstMAI GTCTC 1 cut(s) 1584
BstMBI GATC 4 cut(s) 62, 567, 745, 1300
BstNI CCWGG 7 cut(s) 116, 339, 495, 1149, 1155, 1376, 1540
BstNSI RCATGY 1 cut(s) 77
BstSCI CCNGG 8 cut(s) 114, 337, 493, 1147, 1153, 1374, 1506, 1538
BstSFI CTRYAG 3 cut(s) 512, 641, 663
BstSLI GKGCMC 2 cut(s) 1279, 1507
BsuI GTATCC 1 cut(s) 437
BsuRI GGCC 4 cut(s) 1153, 1551, 1662, 1684
BtgI CCRYGG 1 cut(s) 861
BtrI CACGTC 1 cut(s) 596
BtsCI GGATG 8 cut(s) 217, 334, 789, 988, 1081, 1311, 1469, 1535
BtsI GCAGTG 1 cut(s) 1507
BtsIMutI CAGTG 3 cut(s) 1507, 1574, 1585
BveI ACCTGC 2 cut(s) 467, 572
Cac8I GCNNGC 9 cut(s) 75, 265, 474, 540, 1443, 1467, 1498, 1628, 1686
CaiI CAGNNNCTG 1 cut(s) 881
CfoI GCGC 3 cut(s) 47, 163, 1450
Cfr10I RCCGGY 1 cut(s) 1626
Cfr13I GGNCC 4 cut(s) 410, 1151, 1187, 1513
CseI GACGC 1 cut(s) 593
Csp6I GTAC 3 cut(s) 277, 324, 491
CviAII CATG 9 cut(s) 74, 89, 164, 221, 392, 862, 958, 1211, 1430
CviQI GTAC 3 cut(s) 277, 324, 491
DdeI CTNAG 5 cut(s) 39, 48, 357, 414, 1088
DpnI GATC 4 cut(s) 64, 569, 747, 1302
DpnII GATC 4 cut(s) 62, 567, 745, 1300
DraI TTTAAA 1 cut(s) 1135
Eam1104I CTCTTC 2 cut(s) 618, 1428
EarI CTCTTC 2 cut(s) 618, 1428
Ecl136II GAGCTC 3 cut(s) 38, 83, 1316
Eco130I CCWWGG 1 cut(s) 861
Eco147I AGGCCT 1 cut(s) 1551
Eco24I GRGCYC 4 cut(s) 40, 85, 1318, 1598
Eco32I GATATC 1 cut(s) 1081
Eco47I GGWCC 3 cut(s) 410, 1187, 1513
Eco53kI GAGCTC 3 cut(s) 38, 83, 1316
Eco57I CTGAAG 2 cut(s) 642, 1538
EcoICRI GAGCTC 3 cut(s) 38, 83, 1316
EcoNI CCTNNNNNAGG 1 cut(s) 1152
EcoRII CCWGG 7 cut(s) 114, 337, 493, 1147, 1153, 1374, 1538
EcoRV GATATC 1 cut(s) 1081
EcoT14I CCWWGG 1 cut(s) 861
EcoT22I ATGCAT 1 cut(s) 367
EcoT38I GRGCYC 4 cut(s) 40, 85, 1318, 1598
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 9 cut(s) 77, 92, 167, 224, 395, 865, 961, 1214, 1433
FaqI GGGAC 1 cut(s) 1499
FatI CATG 9 cut(s) 73, 88, 163, 220, 391, 861, 957, 1210, 1429
FauI CCCGC 2 cut(s) 70, 1186
FbaI TGATCA 1 cut(s) 567
FokI GGATG 8 cut(s) 224, 321, 776, 975, 1088, 1318, 1456, 1522
FriOI GRGCYC 4 cut(s) 40, 85, 1318, 1598
FspBI CTAG 6 cut(s) 281, 717, 972, 1442, 1487, 1491
GlaI GCGC 3 cut(s) 46, 162, 1449
GsaI CCCAGC 1 cut(s) 1601
GsuI CTGGAG 2 cut(s) 1203, 1397
HaeII RGCGCY 2 cut(s) 48, 1451
HaeIII GGCC 4 cut(s) 1153, 1551, 1662, 1684
HapII CCGG 3 cut(s) 1508, 1627, 1703
HgaI GACGC 1 cut(s) 593
HhaI GCGC 3 cut(s) 47, 163, 1450
Hin1II CATG 9 cut(s) 77, 92, 167, 224, 395, 865, 961, 1214, 1433
Hin6I GCGC 3 cut(s) 45, 161, 1448
HinP1I GCGC 3 cut(s) 45, 161, 1448
HincII GTYRAC 1 cut(s) 1018
HindII GTYRAC 1 cut(s) 1018
HindIII AAGCTT 3 cut(s) 536, 1262, 1367
HinfI GANTC 6 cut(s) 239, 396, 531, 976, 1140, 1247
HpaII CCGG 3 cut(s) 1508, 1627, 1703
HphI GGTGA 3 cut(s) 266, 452, 878
Hpy166II GTNNAC 3 cut(s) 193, 766, 1018
Hpy188I TCNGA 6 cut(s) 51, 358, 1064, 1091, 1145, 1305
Hpy188III TCNNGA 2 cut(s) 793, 1220
Hpy8I GTNNAC 3 cut(s) 193, 766, 1018
HpyAV CCTTC 5 cut(s) 521, 792, 1262, 1396, 1562
HpyCH4III ACNGT 3 cut(s) 490, 1578, 1589
HpyCH4IV ACGT 2 cut(s) 95, 595
HpyF3I CTNAG 5 cut(s) 39, 48, 357, 414, 1088
HpySE526I ACGT 2 cut(s) 95, 595
Hsp92II CATG 9 cut(s) 77, 92, 167, 224, 395, 865, 961, 1214, 1433
HspAI GCGC 3 cut(s) 45, 161, 1448
KroI GCCGGC 1 cut(s) 1626
KroNI GCCGGC 1 cut(s) 1628
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 4 cut(s) 62, 567, 745, 1300
LmnI GCTCC 3 cut(s) 80, 1438, 1531
LweI GCATC 8 cut(s) 202, 374, 798, 859, 899, 1117, 1478, 1558
MaeI CTAG 6 cut(s) 281, 717, 972, 1442, 1487, 1491
MaeII ACGT 2 cut(s) 95, 595
MaeIII GTNAC 2 cut(s) 994, 1643
MalI GATC 4 cut(s) 64, 569, 747, 1302
MboI GATC 4 cut(s) 62, 567, 745, 1300
MboII GAAGA 5 cut(s) 635, 830, 1040, 1136, 1445
MfeI CAATTG 2 cut(s) 1101, 1296
MhlI GDGCHC 8 cut(s) 40, 85, 521, 1089, 1279, 1318, 1507, 1598
MluCI AATT 8 cut(s) 15, 135, 227, 728, 848, 1035, 1101, 1296
MmeI TCCRAC 3 cut(s) 509, 892, 1168
Mph1103I ATGCAT 1 cut(s) 367
MroNI GCCGGC 1 cut(s) 1626
MroXI GAANNNNTTC 1 cut(s) 1035
MseI TTAA 3 cut(s) 26, 1134, 1320
MslI CAYNNNNRTG 1 cut(s) 962
MspA1I CMGCKG 3 cut(s) 668, 1181, 1601
MspI CCGG 3 cut(s) 1508, 1627, 1703
MspR9I CCNGG 8 cut(s) 116, 339, 495, 1149, 1155, 1376, 1508, 1540
MunI CAATTG 2 cut(s) 1101, 1296
Mva1269I GAATGC 2 cut(s) 367, 1096
MvaI CCWGG 7 cut(s) 116, 339, 495, 1149, 1155, 1376, 1540
NaeI GCCGGC 1 cut(s) 1628
NciI CCSGG 1 cut(s) 1508
NcoI CCATGG 1 cut(s) 861
NdeII GATC 4 cut(s) 62, 567, 745, 1300
NgoMIV GCCGGC 1 cut(s) 1626
NheI GCTAGC 1 cut(s) 1441
NlaIII CATG 9 cut(s) 77, 92, 167, 224, 395, 865, 961, 1214, 1433
NlaIV GGNNCC 8 cut(s) 288, 803, 916, 946, 1233, 1278, 1344, 1515
NmuCI GTSAC 2 cut(s) 994, 1643
NsiI ATGCAT 1 cut(s) 367
NspI RCATGY 1 cut(s) 77
NspV TTCGAA 1 cut(s) 154
PaeI GCATGC 1 cut(s) 77
PaqCI CACCTGC 2 cut(s) 467, 572
PceI AGGCCT 1 cut(s) 1551
PcsI WCGNNNNNNNCGW 2 cut(s) 151, 601
PctI GAATGC 2 cut(s) 367, 1096
PdiI GCCGGC 1 cut(s) 1628
PdmI GAANNNNTTC 1 cut(s) 1035
PfeI GAWTC 6 cut(s) 239, 396, 531, 976, 1140, 1247
PfoI TCCNGGA 2 cut(s) 337, 1374
Ppu21I YACGTR 1 cut(s) 96
PsiI TTATAA 1 cut(s) 1334
Psp124BI GAGCTC 3 cut(s) 40, 85, 1318
Psp6I CCWGG 7 cut(s) 114, 337, 493, 1147, 1153, 1374, 1538
PspFI CCCAGC 1 cut(s) 1597
PspGI CCWGG 7 cut(s) 114, 337, 493, 1147, 1153, 1374, 1538
PspN4I GGNNCC 8 cut(s) 288, 803, 916, 946, 1233, 1278, 1344, 1515
PspPI GGNCC 4 cut(s) 410, 1151, 1187, 1513
PstI CTGCAG 1 cut(s) 667
PstNI CAGNNNCTG 1 cut(s) 881
PvuII CAGCTG 2 cut(s) 668, 1601
RsaI GTAC 3 cut(s) 278, 325, 492
RsaNI GTAC 3 cut(s) 277, 324, 491
RseI CAYNNNNRTG 1 cut(s) 962
SacI GAGCTC 3 cut(s) 40, 85, 1318
SaqAI TTAA 3 cut(s) 26, 1134, 1320
Sau3AI GATC 4 cut(s) 62, 567, 745, 1300
Sau96I GGNCC 4 cut(s) 410, 1151, 1187, 1513
ScrFI CCNGG 8 cut(s) 116, 339, 495, 1149, 1155, 1376, 1508, 1540
SduI GDGCHC 8 cut(s) 40, 85, 521, 1089, 1279, 1318, 1507, 1598
SfaNI GCATC 8 cut(s) 202, 374, 798, 859, 899, 1117, 1478, 1558
SfcI CTRYAG 3 cut(s) 512, 641, 663
SfuI TTCGAA 1 cut(s) 154
SinI GGWCC 3 cut(s) 410, 1187, 1513
SmiMI CAYNNNNRTG 1 cut(s) 962
SphI GCATGC 1 cut(s) 77
Sse9I AATT 8 cut(s) 15, 135, 227, 728, 848, 1035, 1101, 1296
SseBI AGGCCT 1 cut(s) 1551
SsiI CCGC 5 cut(s) 77, 885, 1179, 1614, 1650
SspMI CTAG 6 cut(s) 281, 717, 972, 1442, 1487, 1491
SstI GAGCTC 3 cut(s) 40, 85, 1318
StuI AGGCCT 1 cut(s) 1551
StyD4I CCNGG 8 cut(s) 114, 337, 493, 1147, 1153, 1374, 1506, 1538
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 3 cut(s) 490, 1578, 1589
TaiI ACGT 2 cut(s) 98, 598
TaqI TCGA 1 cut(s) 154
TasI AATT 8 cut(s) 15, 135, 227, 728, 848, 1035, 1101, 1296
TatI WGTACW 1 cut(s) 323
TauI GCSGC 3 cut(s) 887, 1617, 1653
TfiI GAWTC 6 cut(s) 239, 396, 531, 976, 1140, 1247
Tru1I TTAA 3 cut(s) 26, 1134, 1320
Tru9I TTAA 3 cut(s) 26, 1134, 1320
TscAI CASTG 3 cut(s) 1507, 1581, 1592
TseFI GTSAC 2 cut(s) 994, 1643
Tsp45I GTSAC 2 cut(s) 994, 1643
TspDTI ATGAA 8 cut(s) 209, 252, 323, 482, 946, 980, 1322, 1446
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 3 cut(s) 1507, 1581, 1592
VpaK11BI GGWCC 3 cut(s) 410, 1187, 1513
XagI CCTNNNNNAGG 1 cut(s) 1152
XapI RAATTY 2 cut(s) 728, 1035
XceI RCATGY 1 cut(s) 77
XmnI GAANNNNTTC 1 cut(s) 1035
XspI CTAG 6 cut(s) 281, 717, 972, 1442, 1487, 1491
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.