pycom01g04970

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004128
Physical Location & Seq
Reverse (-)
4351542 .. 4357974
6433 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g04970.1

Sequence Viewer

Length: 1587 bp
ATGCCCATTTGTAAGACTGTCACTCCAACTCCCGGCTCATTTTTTTGGAGAACTAGAGAGATCCGAAGTTCCCAATGTAGCTTTATGGTAGGAAATAAAGTCAGCTTTCTAAGGCAGAGAGCTCAGGGTGCTCAAGTTTGTTTTAAATCCAGGAAGCATGGGGGAGCTCTCCATGCTACATGTCGTGCTGAAAAAATTCTGGTGGCAAACAGAGGAGAAATTGCTGTTCGCATTATTAGAACTGCCCATGAAATGGGAATACCTTGTGTGGCAGTTTACTCGACAATAGATAAGGATGCACTTCATGTGAAATTGGCTGATGAATCAGTTTGTATTGGTGAAGCTGCAAGCAGTCAGTCGTACTTAGTTGTTCCAAATGTTTTATCTGCTGCCATCAGTCGGAAATGTACAATGCTGCATCCGGGATACGGTTTCCTTGCTGAGAATGCATCTTTTGTTGAAATGTGCAGAGAACATGGAATCAACTTTATTGGGCCTAAGCCTGATAGCATTCGTGTTATGGGTGATAAATCGTCTGCCAGGGACACAATGAAGAAAGCTGGCGTTCCAACTGTACCAGGAAGTGATGGATTACTACAGAGCACAGGGGAAGGAATCAGGCTTGCAGATGAGATTGGTTTTCCTGTTATGATCAAGGCAACAGCAGGAGGTGGAGGACGTGGCATGCGTCTTGCTAAAGAACCTGAGGAGTTTGTGAAGCTGTTACAGCAAGCAAAAAGTGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTCTATTTGGAAAAGTACATCCAAAATCCTAGACACATTGAATTTCAGGTCTTAGCAGATAAATATGGAAATGTTGTTCACTTTGGGGAGCGAGATTGCAGCATCCAGAGAAGGAATCAAAAGCTGCTGGAAGAAGCACCCTCCCCTGCATTGACCCCGGAATTGCGAAAAGCTATGGGTGACGCAGCAGTTGCTGCAGCAGCATCGATTGGTTACATTGGAGTTGGAACCGTTGAATTCCTTTTGGATGAAAGAGGTTCCTTCTACTTCATGGAAATGAACACTCGGATTCAGGTTGAGCATCCTGTGACAGAAATGATTTCATCTGTTGACTTGATTGAAGAACAAATTCGTGTAGCTATGGGAGAGAAGCTCCGATACACACAGGATGAGATAGTGCTCAGAGGACATTCAATTGAATGTCGTATCAATGCAGAAGATGCTTTTAAAGGATTCCGACCTGGGCCAGGAAGAATAACAGCCTACTTACCATCTGGAGGTCCCTTTGTTAGAATGGATAGCCATGTTTATCCCGATTATGTGGTTCCTCCAAACTATGATTCCCTTCTTGGAAAGCTTATTGTATGGGCACCAACAAGAGAAAAGGCAATTGAACGAATGAAGAGGGCTCTTGACGACACTATTATAACAGGTGTTCCGACAACCATTGAATATCATAAACTTATCCTTGATATAGAGGATTTCAAAAATGGCGAAGTTGATACTGCTTTTATCCCAAAGCATGAGGATGAGTTACAAGCGCCCCAACATTTGATGCCTGCACCTCCCAAAGAACTAGCTGGTGCATCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

529

Amino Acids

57.98

Weight (kDa)

6.72

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 64 - 171 3.5e-41 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 176 - 383 9.6e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 199 - 353 4.9e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 208 - 352 2.1e-06 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 397 - 503 3.3e-38 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1418
AccB1I GGYRCC 1 cut(s) 1360
AccB7I CCANNNNNTGG 1 cut(s) 253
AclWI GGATC 1 cut(s) 55
AcsI RAATTY 4 cut(s) 195, 812, 1007, 1119
AfaI GTAC 4 cut(s) 362, 409, 576, 788
AfiI CCNNNNNNNGG 6 cut(s) 32, 253, 399, 428, 1238, 1268
AflIII ACRYGT 1 cut(s) 179
AgsI TTSAA 9 cut(s) 461, 812, 1007, 1112, 1185, 1190, 1385, 1442, 1477
AjiI CACGTC 1 cut(s) 680
AjnI CCWGG 5 cut(s) 149, 539, 577, 1231, 1237
AjuI GAANNNNNNNTTGG 2 cut(s) 1315, 1347
AloI GAACNNNNNNTCC 2 cut(s) 831, 863
Alw21I GWGCWC 5 cut(s) 124, 133, 169, 605, 1173
AlwI GGATC 1 cut(s) 55
AlwNI CAGNNNCTG 1 cut(s) 965
AoxI GGCC 2 cut(s) 494, 1235
ApoI RAATTY 4 cut(s) 195, 812, 1007, 1119
Asp700I GAANNNNTTC 2 cut(s) 195, 1119
AspLEI GCGC 1 cut(s) 1534
AspS9I GGNCC 3 cut(s) 494, 1235, 1271
AsuC2I CCSGG 3 cut(s) 33, 423, 929
AsuHPI GGTGA 3 cut(s) 350, 536, 962
AvaII GGWCC 1 cut(s) 1271
AxyI CCTNAGG 1 cut(s) 705
BaeGI GKGCMC 1 cut(s) 1363
BanI GGYRCC 1 cut(s) 1360
BanII GRGCYC 3 cut(s) 124, 169, 1402
BarI GAAGNNNNNNTAC 2 cut(s) 1133, 1165
Bbv12I GWGCWC 5 cut(s) 124, 133, 169, 605, 1173
BccI CCATC 4 cut(s) 401, 581, 761, 1270
BcgI CGANNNNNNTGC 4 cut(s) 261, 295, 957, 991
BciT130I CCWGG 5 cut(s) 151, 541, 579, 1233, 1239
BciVI GTATCC 1 cut(s) 419
BclI TGATCA 1 cut(s) 651
BcnI CCSGG 3 cut(s) 33, 423, 929
BfaI CTAG 3 cut(s) 54, 801, 1568
BfmI CTRYAG 3 cut(s) 596, 747, 966
BfoI RGCGCY 1 cut(s) 1535
BfuI GTATCC 1 cut(s) 419
Bme1390I CCNGG 8 cut(s) 33, 151, 423, 541, 579, 929, 1233, 1239
Bme18I GGWCC 1 cut(s) 1271
BmgBI CACGTC 1 cut(s) 680
BmgT120I GGNCC 3 cut(s) 494, 1235, 1271
BmiI GGNNCC 5 cut(s) 1000, 1030, 1273, 1317, 1362
BmrFI CCNGG 8 cut(s) 33, 151, 423, 541, 579, 929, 1233, 1239
BmsI GCATC 8 cut(s) 286, 427, 458, 882, 983, 1081, 1201, 1536
BplI GAGNNNNNCTC 2 cut(s) 1128, 1160
BpmI CTGGAG 1 cut(s) 1287
Bpu10I CCTNAGC 2 cut(s) 123, 498
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 3 cut(s) 33, 423, 929
Bsa29I ATCGAT 1 cut(s) 977
BsaJI CCNNGG 3 cut(s) 540, 927, 1232
BsaXI ACNNNNNCTCC 4 cut(s) 7, 13, 37, 43
Bsc4I CCNNNNNNNGG 6 cut(s) 32, 253, 399, 428, 1238, 1268
Bse21I CCTNAGG 1 cut(s) 705
BseBI CCWGG 5 cut(s) 151, 541, 579, 1233, 1239
BseCI ATCGAT 1 cut(s) 977
BseDI CCNNGG 3 cut(s) 540, 927, 1232
BseGI GGATG 8 cut(s) 301, 418, 789, 873, 1024, 1072, 1165, 1525
BseLI CCNNNNNNNGG 6 cut(s) 32, 253, 399, 428, 1238, 1268
BseMII CTCAG 4 cut(s) 137, 432, 696, 1186
BseRI GAGGAG 2 cut(s) 228, 722
BseSI GKGCMC 1 cut(s) 1363
BsgI GTGCAG 2 cut(s) 487, 1536
BshFI GGCC 2 cut(s) 496, 1237
BshNI GGYRCC 1 cut(s) 1360
BshVI ATCGAT 1 cut(s) 977
BsiHKAI GWGCWC 5 cut(s) 124, 133, 169, 605, 1173
BsiSI CCGG 3 cut(s) 33, 422, 929
BslFI GGGAC 2 cut(s) 557, 1257
BslI CCNNNNNNNGG 6 cut(s) 32, 253, 399, 428, 1238, 1268
BsmFI GGGAC 2 cut(s) 557, 1257
BsmI GAATGC 2 cut(s) 451, 510
BsnI GGCC 2 cut(s) 496, 1237
Bsp1286I GDGCHC 7 cut(s) 124, 133, 169, 605, 1173, 1363, 1402
Bsp1407I TGTACA 1 cut(s) 407
Bsp143I GATC 2 cut(s) 60, 651
BspANI GGCC 2 cut(s) 496, 1237
BspCNI CTCAG 4 cut(s) 136, 433, 697, 1185
BspDI ATCGAT 1 cut(s) 977
BspLI GGNNCC 5 cut(s) 1000, 1030, 1273, 1317, 1362
BspMAI CTGCAG 2 cut(s) 751, 970
BspPI GGATC 1 cut(s) 55
BspT107I GGYRCC 1 cut(s) 1360
BsrGI TGTACA 1 cut(s) 407
BssECI CCNNGG 3 cut(s) 540, 927, 1232
BssMI GATC 2 cut(s) 60, 651
Bst2UI CCWGG 5 cut(s) 151, 541, 579, 1233, 1239
Bst4CI ACNGT 4 cut(s) 19, 431, 574, 1003
Bst6I CTCTTC 1 cut(s) 1388
BstAPI GCANNNNNTGC 3 cut(s) 962, 965, 1211
BstAUI TGTACA 1 cut(s) 407
BstC8I GCNNGC 6 cut(s) 349, 562, 624, 686, 732, 1551
BstDEI CTNAG 9 cut(s) 110, 123, 364, 441, 498, 705, 823, 1172, 1584
BstENI CCTNNNNNAGG 1 cut(s) 1236
BstF5I GGATG 8 cut(s) 301, 418, 789, 873, 1024, 1072, 1165, 1525
BstH2I RGCGCY 1 cut(s) 1535
BstHHI GCGC 1 cut(s) 1534
BstKTI GATC 2 cut(s) 63, 654
BstMBI GATC 2 cut(s) 60, 651
BstMWI GCNNNNNNNGC 9 cut(s) 128, 173, 446, 727, 752, 962, 965, 971, 1211
BstNI CCWGG 5 cut(s) 151, 541, 579, 1233, 1239
BstNSI RCATGY 2 cut(s) 183, 688
BstSCI CCNGG 8 cut(s) 31, 149, 421, 539, 577, 927, 1231, 1237
BstSFI CTRYAG 3 cut(s) 596, 747, 966
BstSLI GKGCMC 1 cut(s) 1363
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
Bsu15I ATCGAT 1 cut(s) 977
Bsu36I CCTNAGG 1 cut(s) 705
BsuI GTATCC 1 cut(s) 419
BsuRI GGCC 2 cut(s) 496, 1237
BsuTUI ATCGAT 1 cut(s) 977
BtrI CACGTC 1 cut(s) 680
BtsCI GGATG 8 cut(s) 301, 418, 789, 873, 1024, 1072, 1165, 1525
Cac8I GCNNGC 6 cut(s) 349, 562, 624, 686, 732, 1551
CaiI CAGNNNCTG 1 cut(s) 965
CfoI GCGC 1 cut(s) 1534
Cfr13I GGNCC 3 cut(s) 494, 1235, 1271
ClaI ATCGAT 1 cut(s) 977
CseI GACGC 2 cut(s) 677, 962
Csp6I GTAC 4 cut(s) 361, 408, 575, 787
CviQI GTAC 4 cut(s) 361, 408, 575, 787
DdeI CTNAG 9 cut(s) 110, 123, 364, 441, 498, 705, 823, 1172, 1584
DpnI GATC 2 cut(s) 62, 653
DpnII GATC 2 cut(s) 60, 651
DraI TTTAAA 2 cut(s) 145, 1219
Eam1104I CTCTTC 1 cut(s) 1388
EarI CTCTTC 1 cut(s) 1388
Ecl136II GAGCTC 2 cut(s) 122, 167
Eco24I GRGCYC 3 cut(s) 124, 169, 1402
Eco47I GGWCC 1 cut(s) 1271
Eco53kI GAGCTC 2 cut(s) 122, 167
Eco81I CCTNAGG 1 cut(s) 705
EcoICRI GAGCTC 2 cut(s) 122, 167
EcoNI CCTNNNNNAGG 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 1271
EcoRI GAATTC 1 cut(s) 1007
EcoRII CCWGG 5 cut(s) 149, 539, 577, 1231, 1237
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 3 cut(s) 124, 169, 1402
FaqI GGGAC 2 cut(s) 557, 1257
FbaI TGATCA 1 cut(s) 651
FokI GGATG 8 cut(s) 308, 405, 776, 860, 1031, 1059, 1172, 1532
FriOI GRGCYC 3 cut(s) 124, 169, 1402
FspBI CTAG 3 cut(s) 54, 801, 1568
GlaI GCGC 1 cut(s) 1533
GsuI CTGGAG 1 cut(s) 1287
HaeII RGCGCY 1 cut(s) 1535
HaeIII GGCC 2 cut(s) 496, 1237
HapII CCGG 3 cut(s) 33, 422, 929
HgaI GACGC 2 cut(s) 677, 962
HhaI GCGC 1 cut(s) 1534
Hin6I GCGC 1 cut(s) 1532
HinP1I GCGC 1 cut(s) 1532
HincII GTYRAC 1 cut(s) 1102
HindII GTYRAC 1 cut(s) 1102
HindIII AAGCTT 1 cut(s) 1346
HinfI GANTC 8 cut(s) 323, 480, 615, 771, 886, 1060, 1224, 1331
HpaII CCGG 3 cut(s) 33, 422, 929
HphI GGTGA 3 cut(s) 350, 536, 962
Hpy166II GTNNAC 3 cut(s) 277, 850, 1102
Hpy188I TCNGA 7 cut(s) 65, 402, 1059, 1148, 1175, 1229, 1431
Hpy188III TCNNGA 4 cut(s) 877, 1266, 1304, 1403
Hpy8I GTNNAC 3 cut(s) 277, 850, 1102
HpyAV CCTTC 4 cut(s) 605, 876, 1042, 1346
HpyCH4III ACNGT 4 cut(s) 19, 431, 574, 1003
HpyCH4IV ACGT 1 cut(s) 679
HpyF10VI GCNNNNNNNGC 9 cut(s) 128, 173, 446, 727, 752, 962, 965, 971, 1211
HpyF3I CTNAG 9 cut(s) 110, 123, 364, 441, 498, 705, 823, 1172, 1584
HpySE526I ACGT 1 cut(s) 679
HspAI GCGC 1 cut(s) 1532
Ksp22I TGATCA 1 cut(s) 651
Kzo9I GATC 2 cut(s) 60, 651
LmnI GCTCC 3 cut(s) 164, 859, 1149
LweI GCATC 8 cut(s) 286, 427, 458, 882, 983, 1081, 1201, 1536
MaeI CTAG 3 cut(s) 54, 801, 1568
MaeII ACGT 1 cut(s) 679
MaeIII GTNAC 6 cut(s) 19, 723, 950, 983, 1078, 1524
MalI GATC 2 cut(s) 62, 653
MboI GATC 2 cut(s) 60, 651
MboII GAAGA 6 cut(s) 565, 914, 1124, 1220, 1254, 1405
MfeI CAATTG 2 cut(s) 1185, 1380
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 7 cut(s) 124, 133, 169, 605, 1173, 1363, 1402
MluCI AATT 9 cut(s) 195, 219, 311, 812, 932, 1007, 1119, 1185, 1380
MlyI GAGTC 1 cut(s) 780
MmeI TCCRAC 6 cut(s) 50, 380, 593, 976, 1252, 1454
Mph1103I ATGCAT 1 cut(s) 451
MroXI GAANNNNTTC 2 cut(s) 195, 1119
MseI TTAA 2 cut(s) 144, 1218
MslI CAYNNNNRTG 2 cut(s) 1046, 1518
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 3 cut(s) 33, 422, 929
MspR9I CCNGG 8 cut(s) 33, 151, 423, 541, 579, 929, 1233, 1239
MunI CAATTG 2 cut(s) 1185, 1380
Mva1269I GAATGC 2 cut(s) 451, 510
MvaI CCWGG 5 cut(s) 151, 541, 579, 1233, 1239
MwoI GCNNNNNNNGC 9 cut(s) 128, 173, 446, 727, 752, 962, 965, 971, 1211
NciI CCSGG 3 cut(s) 33, 423, 929
NdeII GATC 2 cut(s) 60, 651
NlaIV GGNNCC 5 cut(s) 1000, 1030, 1273, 1317, 1362
NmuCI GTSAC 3 cut(s) 19, 950, 1078
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 2 cut(s) 183, 688
PaeI GCATGC 1 cut(s) 688
PciI ACATGT 1 cut(s) 179
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PctI GAATGC 2 cut(s) 451, 510
PdmI GAANNNNTTC 2 cut(s) 195, 1119
PfeI GAWTC 7 cut(s) 323, 480, 615, 886, 1060, 1224, 1331
PflMI CCANNNNNTGG 1 cut(s) 253
PfoI TCCNGGA 2 cut(s) 149, 421
PleI GAGTC 1 cut(s) 779
PpsI GAGTC 1 cut(s) 779
PpuMI RGGWCCY 1 cut(s) 1271
PscI ACATGT 1 cut(s) 179
PsiI TTATAA 1 cut(s) 1418
Psp124BI GAGCTC 2 cut(s) 124, 169
Psp5II RGGWCCY 1 cut(s) 1271
Psp6I CCWGG 5 cut(s) 149, 539, 577, 1231, 1237
PspGI CCWGG 5 cut(s) 149, 539, 577, 1231, 1237
PspN4I GGNNCC 5 cut(s) 1000, 1030, 1273, 1317, 1362
PspPI GGNCC 3 cut(s) 494, 1235, 1271
PspPPI RGGWCCY 1 cut(s) 1271
PstI CTGCAG 2 cut(s) 751, 970
PstNI CAGNNNCTG 1 cut(s) 965
PsuI RGATCY 1 cut(s) 60
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 4 cut(s) 362, 409, 576, 788
RsaNI GTAC 4 cut(s) 361, 408, 575, 787
RseI CAYNNNNRTG 2 cut(s) 1046, 1518
SacI GAGCTC 2 cut(s) 124, 169
SaqAI TTAA 2 cut(s) 144, 1218
Sau3AI GATC 2 cut(s) 60, 651
Sau96I GGNCC 3 cut(s) 494, 1235, 1271
SchI GAGTC 1 cut(s) 780
ScrFI CCNGG 8 cut(s) 33, 151, 423, 541, 579, 929, 1233, 1239
SduI GDGCHC 7 cut(s) 124, 133, 169, 605, 1173, 1363, 1402
SfaNI GCATC 8 cut(s) 286, 427, 458, 882, 983, 1081, 1201, 1536
SfcI CTRYAG 3 cut(s) 596, 747, 966
SinI GGWCC 1 cut(s) 1271
SmiMI CAYNNNNRTG 2 cut(s) 1046, 1518
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
SphI GCATGC 1 cut(s) 688
Sse9I AATT 9 cut(s) 195, 219, 311, 812, 932, 1007, 1119, 1185, 1380
SspMI CTAG 3 cut(s) 54, 801, 1568
SstI GAGCTC 2 cut(s) 124, 169
StyD4I CCNGG 8 cut(s) 31, 149, 421, 539, 577, 927, 1231, 1237
TaaI ACNGT 4 cut(s) 19, 431, 574, 1003
TaiI ACGT 1 cut(s) 682
TaqI TCGA 2 cut(s) 281, 977
TasI AATT 9 cut(s) 195, 219, 311, 812, 932, 1007, 1119, 1185, 1380
TatI WGTACW 2 cut(s) 407, 786
TfiI GAWTC 7 cut(s) 323, 480, 615, 886, 1060, 1224, 1331
Tru1I TTAA 2 cut(s) 144, 1218
Tru9I TTAA 2 cut(s) 144, 1218
TseFI GTSAC 3 cut(s) 19, 950, 1078
Tsp45I GTSAC 3 cut(s) 19, 950, 1078
TspDTI ATGAA 9 cut(s) 264, 293, 336, 566, 1030, 1035, 1064, 1083, 1406
Van91I CCANNNNNTGG 1 cut(s) 253
VpaK11BI GGWCC 1 cut(s) 1271
XagI CCTNNNNNAGG 1 cut(s) 1236
XapI RAATTY 4 cut(s) 195, 812, 1007, 1119
XceI RCATGY 2 cut(s) 183, 688
XmnI GAANNNNTTC 2 cut(s) 195, 1119
XspI CTAG 3 cut(s) 54, 801, 1568
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.