FvH4_4g06120

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004128
Physical Location & Seq
Reverse (-)
5499346 .. 5505281
5936 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06120.t1

Sequence Viewer

Length: 1602 bp
ATGGATGCCACCATGCCCATTTGCAAGACTGTTTCTCCAACTCCGGGGTTATTTTTTGGAAAAACTGGAGAGATCAGGAGTTCCCAATGTAGCTTCATGGTGGGAAATAAAGTTAGCTTTTTGAGGCAGAGAGCTCAGGGTGCTCAAATTAGTTATAGATCAAGGAAGCATGCGGGAACTCTCCATGCTACATGTCGTGCTGATAAAATCCTGGTGGCAAATAGAGGAGAAATTGCAGTTCGTGTTATTCGAACGGCGCATGAGATGGGCATACCCTGTGTGGCTGTCTACTCAACAATAGACAAGGATGCGCTTCATGTCAAATTGGCTGATGAGTCGGTTTGTATTGGTGAAGCAGCAAGCAATCAATCGTACCTTGTAGTTCCAAATGTTTTATCTGCTGCCATCAGTCGTAAATGTACAATGCTTCATCCTGGATATGGTTTCCTATCTGAGAATGCATCTTTTGTTGAAATGTGCAAAGAACATGGAATCAACTTTATTGGTCCTAAGCCTGAAAGTATCCGTATTATGGGTGATAAATCCACTGCAAGAGAAACAATGAAGCAAGCAGGTGTTCCAACTGTACCCGGAAGTGATGGATTACTAAAGAGCACAGAGGAAGGAATGAAGCTTGCTGATGAGATTGGTTATCCTGTAATGATCAAGGCAACAGCAGGTGGTGGAGGACGTGGCATGCGTCTTGCTAAAGAGCCTGAGGAGTTTGTAAAGTTGTTACAGGCAGCAAAGAGCGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTATGTCGTAAATCCTAGACACATTGAATTTCAGGTTCTTGCAGATAAGTACGGTAATGTTGTTCACTTTGGAGAACGTGATTGCAGCATCCAGAGAAGGAACCAAAAGCTTCTAGAAGAAGCACCCTCTCCTGCATTAACTCCAGAACTGCGGAAAGCCATGGGTGATGCCGCAGTTGCTGCCGCAGCATCTATTGGTTACATTGGTGTTGGAACCGTTGAGTTCCTTCTGGACGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTAGGATTCAGGTTGAGCATCCTGTGACAGAAATGATTTCCTCTGTTGACTTGATTGAAGAACAAATTCGTGTTGCTATGGGAGAAAAACTCAGAATGACTCAGGATGATATCGTTCTCCGAGGACATTCAATTGAATGTCGTATCAATGCAGAAGACGCTTTTAAAGGATTCCGACCTGGGCCAGGTAGAATAACAGCTTACTTACCCGCTGGAGGTCCATTTGTTAGAATGGATAGTCATGTTTATCCTGATTATGTGGTTCCTCCAAACTATGATTCCCTTCTTGGAAAGCTTATTGTGTGGGCACCAACAAGAGAAAAGGCAATTGATCGGATGAAAAGAGCTCTTAACGACACTATTATAACAGGGGTTCCAACAACTATTGAATATCATAAGCTCATCCTGGAGATAGAGGATTTCAAAAATGGGAAGGTTGATACCGCTTTTATTCCCAAGCATGAAGAGGAGCTAGCAGCGCCCCAACAAGTGTTGCCTGCATCCCCACCGAAAGAACTAGCTAGTGCAAGTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

534

Amino Acids

58.23

Weight (kDa)

6.69

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 68 - 175 1.1e-41 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 180 - 387 2.3e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 189 - 357 1.7e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 206 - 356 3.7e-07 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 401 - 507 2.6e-38 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1430
AarI CACCTGC 2 cut(s) 563, 668
Acc36I ACCTGC 2 cut(s) 563, 668
AccB1I GGYRCC 1 cut(s) 1372
AccI GTMKAC 1 cut(s) 288
AciI CCGC 6 cut(s) 173, 949, 969, 981, 1275, 1509
AcsI RAATTY 2 cut(s) 824, 1131
AfaI GTAC 4 cut(s) 374, 421, 588, 848
AfiI CCNNNNNNNGG 5 cut(s) 44, 440, 532, 1250, 1280
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 7 cut(s) 473, 824, 1124, 1197, 1202, 1454, 1489
AjiI CACGTC 1 cut(s) 692
AjnI CCWGG 5 cut(s) 210, 433, 1243, 1249, 1470
AjuI GAANNNNNNNTTGG 2 cut(s) 1327, 1359
Alw21I GWGCWC 4 cut(s) 136, 145, 617, 1414
AlwNI CAGNNNCTG 1 cut(s) 977
AoxI GGCC 1 cut(s) 1247
ApoI RAATTY 2 cut(s) 824, 1131
Asp700I GAANNNNTTC 1 cut(s) 1131
AspLEI GCGC 3 cut(s) 259, 313, 1546
AspS9I GGNCC 3 cut(s) 506, 1247, 1283
AsuC2I CCSGG 2 cut(s) 45, 591
AsuHPI GGTGA 3 cut(s) 362, 548, 974
AsuII TTCGAA 1 cut(s) 250
AsuNHI GCTAGC 1 cut(s) 1537
AvaII GGWCC 2 cut(s) 506, 1283
AxyI CCTNAGG 1 cut(s) 717
BaeGI GKGCMC 1 cut(s) 1375
BanI GGYRCC 1 cut(s) 1372
BanII GRGCYC 2 cut(s) 136, 1414
BbsI GAAGAC 1 cut(s) 1227
Bbv12I GWGCWC 4 cut(s) 136, 145, 617, 1414
BccI CCATC 4 cut(s) 259, 413, 593, 773
BceAI ACGGC 1 cut(s) 270
BciT130I CCWGG 5 cut(s) 212, 435, 1245, 1251, 1472
BciVI GTATCC 1 cut(s) 533
BclI TGATCA 1 cut(s) 663
BcnI CCSGG 2 cut(s) 45, 591
BfaI CTAG 6 cut(s) 813, 911, 1068, 1538, 1583, 1587
BfmI CTRYAG 1 cut(s) 759
BfoI RGCGCY 1 cut(s) 1547
BfuAI ACCTGC 2 cut(s) 563, 668
BfuI GTATCC 1 cut(s) 533
Bme1390I CCNGG 7 cut(s) 45, 212, 435, 591, 1245, 1251, 1472
Bme18I GGWCC 2 cut(s) 506, 1283
BmgBI CACGTC 1 cut(s) 692
BmgT120I GGNCC 3 cut(s) 506, 1247, 1283
BmiI GGNNCC 6 cut(s) 899, 1012, 1042, 1329, 1374, 1440
BmrFI CCNGG 7 cut(s) 45, 212, 435, 591, 1245, 1251, 1472
BmsI GCATC 7 cut(s) 298, 470, 894, 955, 995, 1093, 1574
BmtI GCTAGC 1 cut(s) 1541
BpiI GAAGAC 1 cut(s) 1227
BplI GAGNNNNNCTC 2 cut(s) 1140, 1172
BpmI CTGGAG 4 cut(s) 87, 924, 1299, 1493
Bpu10I CCTNAGC 2 cut(s) 135, 510
Bpu14I TTCGAA 1 cut(s) 250
BpuMI CCSGG 2 cut(s) 45, 591
BsaJI CCNNGG 4 cut(s) 44, 957, 1186, 1244
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 440, 532, 1250, 1280
Bse1I ACTGG 1 cut(s) 70
Bse21I CCTNAGG 1 cut(s) 717
BseBI CCWGG 5 cut(s) 212, 435, 1245, 1251, 1472
BseDI CCNNGG 4 cut(s) 44, 957, 1186, 1244
BseGI GGATG 9 cut(s) 10, 313, 430, 885, 1084, 1177, 1407, 1467, 1565
BseLI CCNNNNNNNGG 5 cut(s) 44, 440, 532, 1250, 1280
BseMII CTCAG 5 cut(s) 149, 444, 708, 1171, 1181
BseNI ACTGG 1 cut(s) 70
BseRI GAGGAG 3 cut(s) 240, 734, 1547
BseSI GKGCMC 1 cut(s) 1375
BshFI GGCC 1 cut(s) 1249
BshNI GGYRCC 1 cut(s) 1372
BsiHKAI GWGCWC 4 cut(s) 136, 145, 617, 1414
BsiSI CCGG 2 cut(s) 44, 591
BslI CCNNNNNNNGG 5 cut(s) 44, 440, 532, 1250, 1280
BsmI GAATGC 1 cut(s) 463
BsnI GGCC 1 cut(s) 1249
Bsp119I TTCGAA 1 cut(s) 250
Bsp1286I GDGCHC 5 cut(s) 136, 145, 617, 1375, 1414
Bsp1407I TGTACA 1 cut(s) 419
Bsp143I GATC 4 cut(s) 72, 158, 663, 1396
Bsp19I CCATGG 1 cut(s) 957
BspACI CCGC 6 cut(s) 173, 949, 969, 981, 1275, 1509
BspANI GGCC 1 cut(s) 1249
BspCNI CTCAG 5 cut(s) 148, 445, 709, 1170, 1180
BspLI GGNNCC 6 cut(s) 899, 1012, 1042, 1329, 1374, 1440
BspMAI CTGCAG 1 cut(s) 763
BspMI ACCTGC 2 cut(s) 563, 668
BspOI GCTAGC 1 cut(s) 1541
BspT104I TTCGAA 1 cut(s) 250
BspT107I GGYRCC 1 cut(s) 1372
BsrGI TGTACA 1 cut(s) 419
BsrI ACTGG 1 cut(s) 70
BssECI CCNNGG 4 cut(s) 44, 957, 1186, 1244
BssMI GATC 4 cut(s) 72, 158, 663, 1396
BssT1I CCWWGG 1 cut(s) 957
Bst2UI CCWGG 5 cut(s) 212, 435, 1245, 1251, 1472
Bst4CI ACNGT 4 cut(s) 31, 586, 851, 1015
Bst6I CTCTTC 1 cut(s) 1524
BstAPI GCANNNNNTGC 1 cut(s) 977
BstAUI TGTACA 1 cut(s) 419
BstBI TTCGAA 1 cut(s) 250
BstC8I GCNNGC 7 cut(s) 171, 361, 570, 636, 698, 1539, 1563
BstDEI CTNAG 6 cut(s) 135, 453, 510, 717, 1157, 1167
BstDSI CCRYGG 1 cut(s) 957
BstENI CCTNNNNNAGG 1 cut(s) 1248
BstF5I GGATG 9 cut(s) 10, 313, 430, 885, 1084, 1177, 1407, 1467, 1565
BstH2I RGCGCY 1 cut(s) 1547
BstHHI GCGC 3 cut(s) 259, 313, 1546
BstKTI GATC 4 cut(s) 75, 161, 666, 1399
BstMBI GATC 4 cut(s) 72, 158, 663, 1396
BstMWI GCNNNNNNNGC 7 cut(s) 140, 764, 974, 977, 983, 1223, 1543
BstNI CCWGG 5 cut(s) 212, 435, 1245, 1251, 1472
BstNSI RCATGY 3 cut(s) 173, 195, 700
BstSCI CCNGG 7 cut(s) 43, 210, 433, 589, 1243, 1249, 1470
BstSFI CTRYAG 1 cut(s) 759
BstSLI GKGCMC 1 cut(s) 1375
BstV2I GAAGAC 1 cut(s) 1227
Bsu36I CCTNAGG 1 cut(s) 717
BsuI GTATCC 1 cut(s) 533
BsuRI GGCC 1 cut(s) 1249
BtgI CCRYGG 1 cut(s) 957
BtrI CACGTC 1 cut(s) 692
BtsCI GGATG 9 cut(s) 10, 313, 430, 885, 1084, 1177, 1407, 1467, 1565
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 1 cut(s) 546
BveI ACCTGC 2 cut(s) 563, 668
Cac8I GCNNGC 7 cut(s) 171, 361, 570, 636, 698, 1539, 1563
CaiI CAGNNNCTG 1 cut(s) 977
CfoI GCGC 3 cut(s) 259, 313, 1546
Cfr13I GGNCC 3 cut(s) 506, 1247, 1283
CseI GACGC 2 cut(s) 689, 1232
Csp6I GTAC 4 cut(s) 373, 420, 587, 847
CviQI GTAC 4 cut(s) 373, 420, 587, 847
DdeI CTNAG 6 cut(s) 135, 453, 510, 717, 1157, 1167
DpnI GATC 4 cut(s) 74, 160, 665, 1398
DpnII GATC 4 cut(s) 72, 158, 663, 1396
DraI TTTAAA 1 cut(s) 1231
Eam1104I CTCTTC 1 cut(s) 1524
EarI CTCTTC 1 cut(s) 1524
Ecl136II GAGCTC 2 cut(s) 134, 1412
Eco130I CCWWGG 1 cut(s) 957
Eco24I GRGCYC 2 cut(s) 136, 1414
Eco32I GATATC 1 cut(s) 1177
Eco47I GGWCC 2 cut(s) 506, 1283
Eco53kI GAGCTC 2 cut(s) 134, 1412
Eco81I CCTNAGG 1 cut(s) 717
EcoICRI GAGCTC 2 cut(s) 134, 1412
EcoNI CCTNNNNNAGG 1 cut(s) 1248
EcoRII CCWGG 5 cut(s) 210, 433, 1243, 1249, 1470
EcoRV GATATC 1 cut(s) 1177
EcoT14I CCWWGG 1 cut(s) 957
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 2 cut(s) 136, 1414
ErhI CCWWGG 1 cut(s) 957
FauI CCCGC 2 cut(s) 166, 1282
FbaI TGATCA 1 cut(s) 663
FblI GTMKAC 1 cut(s) 288
FokI GGATG 9 cut(s) 17, 320, 417, 872, 1071, 1184, 1414, 1454, 1552
FriOI GRGCYC 2 cut(s) 136, 1414
FspBI CTAG 6 cut(s) 813, 911, 1068, 1538, 1583, 1587
GlaI GCGC 3 cut(s) 258, 312, 1545
GsuI CTGGAG 4 cut(s) 87, 924, 1299, 1493
HaeII RGCGCY 1 cut(s) 1547
HaeIII GGCC 1 cut(s) 1249
HapII CCGG 2 cut(s) 44, 591
HgaI GACGC 2 cut(s) 689, 1232
HhaI GCGC 3 cut(s) 259, 313, 1546
Hin6I GCGC 3 cut(s) 257, 311, 1544
HinP1I GCGC 3 cut(s) 257, 311, 1544
HincII GTYRAC 1 cut(s) 1114
HindII GTYRAC 1 cut(s) 1114
HindIII AAGCTT 3 cut(s) 632, 905, 1358
HinfI GANTC 6 cut(s) 335, 492, 1072, 1165, 1236, 1343
HpaII CCGG 2 cut(s) 44, 591
HphI GGTGA 3 cut(s) 362, 548, 974
Hpy166II GTNNAC 3 cut(s) 289, 862, 1114
Hpy188I TCNGA 5 cut(s) 454, 1160, 1187, 1241, 1401
Hpy188III TCNNGA 7 cut(s) 76, 889, 911, 941, 1028, 1169, 1316
Hpy8I GTNNAC 3 cut(s) 289, 862, 1114
HpyAV CCTTC 5 cut(s) 617, 888, 1034, 1358, 1492
HpyCH4III ACNGT 4 cut(s) 31, 586, 851, 1015
HpyCH4IV ACGT 2 cut(s) 691, 874
HpyF10VI GCNNNNNNNGC 7 cut(s) 140, 764, 974, 977, 983, 1223, 1543
HpyF3I CTNAG 6 cut(s) 135, 453, 510, 717, 1157, 1167
HpySE526I ACGT 2 cut(s) 691, 874
HspAI GCGC 3 cut(s) 257, 311, 1544
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 4 cut(s) 72, 158, 663, 1396
LmnI GCTCC 1 cut(s) 1534
LweI GCATC 7 cut(s) 298, 470, 894, 955, 995, 1093, 1574
MaeI CTAG 6 cut(s) 813, 911, 1068, 1538, 1583, 1587
MaeII ACGT 2 cut(s) 691, 874
MaeIII GTNAC 3 cut(s) 735, 995, 1090
MalI GATC 4 cut(s) 74, 160, 665, 1398
MboI GATC 4 cut(s) 72, 158, 663, 1396
MboII GAAGA 4 cut(s) 926, 1136, 1232, 1541
MfeI CAATTG 2 cut(s) 1197, 1392
MhlI GDGCHC 5 cut(s) 136, 145, 617, 1375, 1414
MluCI AATT 7 cut(s) 147, 231, 323, 824, 1131, 1197, 1392
MlyI GAGTC 2 cut(s) 344, 1159
MmeI TCCRAC 5 cut(s) 62, 605, 988, 1264, 1466
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 1131
MseI TTAA 3 cut(s) 935, 1230, 1416
MslI CAYNNNNRTG 1 cut(s) 1058
MspA1I CMGCKG 2 cut(s) 764, 1277
MspI CCGG 2 cut(s) 44, 591
MspR9I CCNGG 7 cut(s) 45, 212, 435, 591, 1245, 1251, 1472
MunI CAATTG 2 cut(s) 1197, 1392
Mva1269I GAATGC 1 cut(s) 463
MvaI CCWGG 5 cut(s) 212, 435, 1245, 1251, 1472
MwoI GCNNNNNNNGC 7 cut(s) 140, 764, 974, 977, 983, 1223, 1543
NciI CCSGG 2 cut(s) 45, 591
NcoI CCATGG 1 cut(s) 957
NdeII GATC 4 cut(s) 72, 158, 663, 1396
NheI GCTAGC 1 cut(s) 1537
NlaIV GGNNCC 6 cut(s) 899, 1012, 1042, 1329, 1374, 1440
NmuCI GTSAC 1 cut(s) 1090
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 3 cut(s) 173, 195, 700
NspV TTCGAA 1 cut(s) 250
PaeI GCATGC 2 cut(s) 173, 700
PaqCI CACCTGC 2 cut(s) 563, 668
PciI ACATGT 1 cut(s) 191
PcsI WCGNNNNNNNCGW 2 cut(s) 247, 697
PctI GAATGC 1 cut(s) 463
PdmI GAANNNNTTC 1 cut(s) 1131
PfeI GAWTC 4 cut(s) 492, 1072, 1236, 1343
PfoI TCCNGGA 2 cut(s) 433, 1470
PleI GAGTC 2 cut(s) 343, 1159
PpsI GAGTC 2 cut(s) 343, 1159
PscI ACATGT 1 cut(s) 191
PsiI TTATAA 1 cut(s) 1430
Psp124BI GAGCTC 2 cut(s) 136, 1414
Psp6I CCWGG 5 cut(s) 210, 433, 1243, 1249, 1470
PspGI CCWGG 5 cut(s) 210, 433, 1243, 1249, 1470
PspN4I GGNNCC 6 cut(s) 899, 1012, 1042, 1329, 1374, 1440
PspPI GGNCC 3 cut(s) 506, 1247, 1283
PstI CTGCAG 1 cut(s) 763
PstNI CAGNNNCTG 1 cut(s) 977
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 4 cut(s) 374, 421, 588, 848
RsaNI GTAC 4 cut(s) 373, 420, 587, 847
RseI CAYNNNNRTG 1 cut(s) 1058
SacI GAGCTC 2 cut(s) 136, 1414
SaqAI TTAA 3 cut(s) 935, 1230, 1416
Sau3AI GATC 4 cut(s) 72, 158, 663, 1396
Sau96I GGNCC 3 cut(s) 506, 1247, 1283
SchI GAGTC 2 cut(s) 344, 1159
ScrFI CCNGG 7 cut(s) 45, 212, 435, 591, 1245, 1251, 1472
SduI GDGCHC 5 cut(s) 136, 145, 617, 1375, 1414
SfaNI GCATC 7 cut(s) 298, 470, 894, 955, 995, 1093, 1574
SfcI CTRYAG 1 cut(s) 759
SfuI TTCGAA 1 cut(s) 250
SinI GGWCC 2 cut(s) 506, 1283
SmiMI CAYNNNNRTG 1 cut(s) 1058
SphI GCATGC 2 cut(s) 173, 700
Sse9I AATT 7 cut(s) 147, 231, 323, 824, 1131, 1197, 1392
SsiI CCGC 6 cut(s) 173, 949, 969, 981, 1275, 1509
SspMI CTAG 6 cut(s) 813, 911, 1068, 1538, 1583, 1587
SstI GAGCTC 2 cut(s) 136, 1414
StyD4I CCNGG 7 cut(s) 43, 210, 433, 589, 1243, 1249, 1470
StyI CCWWGG 1 cut(s) 957
TaaI ACNGT 4 cut(s) 31, 586, 851, 1015
TaiI ACGT 2 cut(s) 694, 877
TaqI TCGA 1 cut(s) 250
TasI AATT 7 cut(s) 147, 231, 323, 824, 1131, 1197, 1392
TatI WGTACW 1 cut(s) 419
TauI GCSGC 2 cut(s) 971, 983
TfiI GAWTC 4 cut(s) 492, 1072, 1236, 1343
Tru1I TTAA 3 cut(s) 935, 1230, 1416
Tru9I TTAA 3 cut(s) 935, 1230, 1416
TscAI CASTG 1 cut(s) 553
TseFI GTSAC 1 cut(s) 1090
Tsp45I GTSAC 1 cut(s) 1090
TspDTI ATGAA 9 cut(s) 85, 305, 419, 578, 644, 1042, 1076, 1418, 1542
TspGWI ACGGA 1 cut(s) 515
TspRI CASTG 1 cut(s) 553
VpaK11BI GGWCC 2 cut(s) 506, 1283
XagI CCTNNNNNAGG 1 cut(s) 1248
XapI RAATTY 2 cut(s) 824, 1131
XbaI TCTAGA 1 cut(s) 910
XceI RCATGY 3 cut(s) 173, 195, 700
XmiI GTMKAC 1 cut(s) 288
XmnI GAANNNNTTC 1 cut(s) 1131
XspI CTAG 6 cut(s) 813, 911, 1068, 1538, 1583, 1587
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.