Rorug04G0003600

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004128
Physical Location & Seq
Reverse (-)
715543 .. 716870
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0003600.1

Sequence Viewer

Length: 1176 bp
ATGGTGACCGTTGACGAAGTCCGCAAGGCTCAACGGGCTGAGGGTCCGGCCACCGTCTTGGCCATCGGGACAGCCACTCCTCCCAATTGTGTCGACCAGAGCATATATCCCGACTACTATTTTCGTATAACCAAAAGTGAGCACAAGGTTGAGCTCAAGGAGAAATTCCAGCGCATGTGTGACAAGTCAATGATCAAGAAGCGTTACATGTATTTGACTGAAGAGATCTTGAAAGAGAATCCCAGTATGTGCGAGTACATGGCACCTTCACTTGATGCAAGACAAGATATGCTGATTGCTGAAATTCCAAAGTTGGGCAAAGAGGCTGCCACCAAGGCCATTAAGGAATGGGGTCAGCCCAAGTCTAAAATCACCCACTTGGTCTTTTGTACCACCAGTGGTGTTGACATGCCCGGGGCCGATTACCAGCTCACCAAGCTTTTAGGCCTCCACCCGTCCGTCAAGCGCCTCATGATGTACCAACAAGGTTGTTTCGCCGGCGGCACCGTGCTCCGGTTGGCCAAGGACTTGGCTGAGAACAATCGGGGAGCACGTGTTCTCGTTGTTTGCTCCGAGATCACTGTCGTCACTTTCCGTGGGCCTAGCGACACCCATCTCGATAGTCTTGTGGGCCAAGCCTTGTTCGGTGACGGTGCTGCGGCCATTATTGTTGGGGCTGACCCGCTGTCTGAGATTGAGAAGCCCTTGTTTGAGGTTGTCTCGGCGGCTCAAACTATCCTTCCTGATAGTGATGGGGCCATTGAAGGGCATCTTCGTGAAGTTGGGCTCACATTTCACCTCCTCGAAAATGTTCCCATGCTAATTTCGAAGAACATCGAAAAGAGCCTCAACGAGGCCTTCAAGCCTTTGAACATCACGGACTGGAACTCACTTTTCTGGATTGCACACCCGGGTGGCCCTGCAATTCTGGATCAAGTAGAGGCGAAATTGGCATTGAAGCCCGAAAAGTTAGAAGCAACAAGGCACATATTGTCCGAATACGGCAACATGTCCAGTGCTTGTGTGTTTTTTATTTTGGACGAGGTGAGGAGGAAGTCTGTAGCAAATGGGCACAAGACCACTGGAGAGGGCCTGGAGTGGGGAGTTCTATTTGGGTTTGGGCCTGGGCTCACCGTCGAGACCGTCGTGCTTCACAGTGTTGGTGTCACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

391

Amino Acids

42.92

Weight (kDa)

5.98

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chal_sti_synt_N PF00195 5 - 228 8.1e-124 Chalcone and stilbene synthases, N-terminal domain
Chal_sti_synt_C PF02797 238 - 387 4.4e-69 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 262, 503
AccI GTMKAC 1 cut(s) 93
AciI CCGC 5 cut(s) 22, 501, 659, 683, 725
AclWI GGATC 1 cut(s) 939
AcoI YGGCCR 4 cut(s) 48, 60, 519, 660
AcsI RAATTY 2 cut(s) 164, 303
AcuI CTGAAG 1 cut(s) 240
AcvI CACGTG 1 cut(s) 554
AfaI GTAC 3 cut(s) 257, 391, 479
AfiI CCNNNNNNNGG 5 cut(s) 314, 513, 765, 853, 1099
AflIII ACRYGT 3 cut(s) 207, 553, 1008
AgsI TTSAA 5 cut(s) 232, 764, 862, 871, 958
AhdI GACNNNNNGTC 1 cut(s) 685
AjnI CCWGG 2 cut(s) 1092, 1123
AleI CACNNNNGTG 1 cut(s) 912
AloI GAACNNNNNNTCC 2 cut(s) 540, 572
AluBI AGCT 3 cut(s) 154, 430, 439
AluI AGCT 3 cut(s) 154, 430, 439
Alw21I GWGCWC 4 cut(s) 144, 156, 513, 553
Alw26I GTCTC 2 cut(s) 724, 1133
AlwI GGATC 1 cut(s) 939
Ama87I CYCGRG 2 cut(s) 413, 910
ApeKI GCWGC 2 cut(s) 326, 656
ApoI RAATTY 2 cut(s) 164, 303
Asp700I GAANNNNTTC 1 cut(s) 810
AspLEI GCGC 2 cut(s) 174, 468
AspS9I GGNCC 8 cut(s) 44, 417, 599, 631, 756, 917, 1090, 1121
AsuC2I CCSGG 4 cut(s) 414, 415, 911, 912
AsuHPI GGTGA 7 cut(s) 16, 364, 424, 659, 788, 1057, 1123
AsuII TTCGAA 1 cut(s) 827
AvaI CYCGRG 2 cut(s) 413, 910
AvaII GGWCC 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 1074
BalI TGGCCA 2 cut(s) 62, 521
BanI GGYRCC 2 cut(s) 262, 503
BanII GRGCYC 3 cut(s) 156, 789, 1131
BbrPI CACGTG 1 cut(s) 554
Bbv12I GWGCWC 4 cut(s) 144, 156, 513, 553
BbvCI CCTCAGC 1 cut(s) 39
BbvI GCAGC 2 cut(s) 313, 643
BccI CCATC 3 cut(s) 71, 621, 746
BceAI ACGGC 1 cut(s) 1018
BciT130I CCWGG 2 cut(s) 1094, 1125
BclI TGATCA 1 cut(s) 192
BcnI CCSGG 4 cut(s) 414, 415, 911, 912
BcoDI GTCTC 2 cut(s) 724, 1133
BfaI CTAG 1 cut(s) 603
BfmI CTRYAG 1 cut(s) 1059
BfoI RGCGCY 1 cut(s) 469
BglI GCCNNNNNGGC 1 cut(s) 335
BglII AGATCT 1 cut(s) 225
BisI GCNGC 5 cut(s) 327, 502, 657, 660, 726
BlsI GCNGC 5 cut(s) 328, 503, 658, 661, 727
Bme1390I CCNGG 6 cut(s) 414, 415, 911, 912, 1094, 1125
Bme18I GGWCC 1 cut(s) 44
BmeRI GACNNNNNGTC 1 cut(s) 685
BmeT110I CYCGRG 2 cut(s) 413, 910
BmgT120I GGNCC 8 cut(s) 44, 417, 599, 631, 756, 917, 1090, 1121
BmiI GGNNCC 5 cut(s) 45, 264, 418, 505, 757
BmrFI CCNGG 6 cut(s) 414, 415, 911, 912, 1094, 1125
BmrI ACTGGG 1 cut(s) 237
BmsI GCATC 2 cut(s) 265, 778
BmuI ACTGGG 1 cut(s) 237
BplI GAGNNNNNCTC 2 cut(s) 704, 736
BpmI CTGGAG 2 cut(s) 1104, 1115
Bpu10I CCTNAGC 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 827
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 4 cut(s) 414, 415, 911, 912
BsaAI YACGTR 1 cut(s) 554
BsaI GGTCTC 1 cut(s) 1133
BsaJI CCNNGG 7 cut(s) 333, 413, 414, 522, 595, 910, 1124
BsaWI WCCGGW 1 cut(s) 513
BsaXI ACNNNNNCTCC 4 cut(s) 61, 91, 540, 570
Bsc4I CCNNNNNNNGG 5 cut(s) 314, 513, 765, 853, 1099
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 5 cut(s) 243, 396, 887, 1014, 1087
BseBI CCWGG 2 cut(s) 1094, 1125
BseDI CCNNGG 7 cut(s) 333, 413, 414, 522, 595, 910, 1124
BseLI CCNNNNNNNGG 5 cut(s) 314, 513, 765, 853, 1099
BseMII CTCAG 3 cut(s) 30, 525, 681
BseNI ACTGG 5 cut(s) 243, 396, 887, 1014, 1087
BseRI GAGGAG 3 cut(s) 69, 791, 1063
BseSI GKGCMC 1 cut(s) 1074
BseXI GCAGC 2 cut(s) 313, 643
BshNI GGYRCC 2 cut(s) 262, 503
BsiHKAI GWGCWC 4 cut(s) 144, 156, 513, 553
BsiHKCI CYCGRG 2 cut(s) 413, 910
BsiSI CCGG 5 cut(s) 47, 414, 498, 514, 911
BslFI GGGAC 1 cut(s) 82
BslI CCNNNNNNNGG 5 cut(s) 314, 513, 765, 853, 1099
BsmAI GTCTC 2 cut(s) 724, 1133
BsmFI GGGAC 1 cut(s) 82
Bso31I GGTCTC 1 cut(s) 1133
BsoBI CYCGRG 2 cut(s) 413, 910
Bsp119I TTCGAA 1 cut(s) 827
Bsp1286I GDGCHC 7 cut(s) 144, 156, 513, 553, 789, 1074, 1131
Bsp143I GATC 4 cut(s) 192, 225, 576, 931
BspACI CCGC 5 cut(s) 22, 501, 659, 683, 725
BspCNI CTCAG 3 cut(s) 31, 526, 682
BspHI TCATGA 1 cut(s) 471
BspLI GGNNCC 5 cut(s) 45, 264, 418, 505, 757
BspPI GGATC 1 cut(s) 939
BspT104I TTCGAA 1 cut(s) 827
BspT107I GGYRCC 2 cut(s) 262, 503
BspTNI GGTCTC 1 cut(s) 1133
BsrFI RCCGGY 1 cut(s) 497
BsrI ACTGG 5 cut(s) 243, 396, 887, 1014, 1087
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 7 cut(s) 333, 413, 414, 522, 595, 910, 1124
BssMI GATC 4 cut(s) 192, 225, 576, 931
BssT1I CCWWGG 2 cut(s) 333, 522
Bst2UI CCWGG 2 cut(s) 1094, 1125
Bst4CI ACNGT 8 cut(s) 10, 55, 508, 583, 653, 1135, 1144, 1157
Bst6I CTCTTC 1 cut(s) 216
BstBAI YACGTR 1 cut(s) 554
BstBI TTCGAA 1 cut(s) 827
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 3 cut(s) 39, 534, 690
BstDSI CCRYGG 1 cut(s) 595
BstEII GGTNACC 1 cut(s) 4
BstENI CCTNNNNNAGG 1 cut(s) 851
BstH2I RGCGCY 1 cut(s) 469
BstHHI GCGC 2 cut(s) 174, 468
BstKTI GATC 4 cut(s) 195, 228, 579, 934
BstMAI GTCTC 2 cut(s) 724, 1133
BstMBI GATC 4 cut(s) 192, 225, 576, 931
BstMWI GCNNNNNNNGC 4 cut(s) 35, 335, 436, 950
BstNI CCWGG 2 cut(s) 1094, 1125
BstNSI RCATGY 4 cut(s) 178, 211, 412, 1012
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 6 cut(s) 412, 413, 909, 910, 1092, 1123
BstSFI CTRYAG 1 cut(s) 1059
BstSLI GKGCMC 1 cut(s) 1074
BstV1I GCAGC 2 cut(s) 313, 643
BstX2I RGATCY 1 cut(s) 225
BstXI CCANNNNNNTGG 2 cut(s) 58, 529
BstYI RGATCY 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 595
BtsI GCAGTG 1 cut(s) 1167
BtsIMutI CAGTG 6 cut(s) 403, 579, 1021, 1080, 1162, 1167
Cac8I GCNNGC 1 cut(s) 499
CciI TCATGA 1 cut(s) 471
CfoI GCGC 2 cut(s) 174, 468
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 8 cut(s) 44, 417, 599, 631, 756, 917, 1090, 1121
Cfr9I CCCGGG 2 cut(s) 413, 910
Csp6I GTAC 3 cut(s) 256, 390, 478
CviAII CATG 7 cut(s) 175, 208, 259, 409, 472, 817, 1009
CviQI GTAC 3 cut(s) 256, 390, 478
DdeI CTNAG 3 cut(s) 39, 534, 690
DpnI GATC 4 cut(s) 194, 227, 578, 933
DpnII GATC 4 cut(s) 192, 225, 576, 931
DriI GACNNNNNGTC 1 cut(s) 685
EaeI YGGCCR 4 cut(s) 48, 60, 519, 660
Eam1104I CTCTTC 1 cut(s) 216
Eam1105I GACNNNNNGTC 1 cut(s) 685
EarI CTCTTC 1 cut(s) 216
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 333, 522
Eco147I AGGCCT 2 cut(s) 447, 857
Eco24I GRGCYC 3 cut(s) 156, 789, 1131
Eco31I GGTCTC 1 cut(s) 1133
Eco47I GGWCC 1 cut(s) 44
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 240
Eco72I CACGTG 1 cut(s) 554
Eco88I CYCGRG 2 cut(s) 413, 910
Eco91I GGTNACC 1 cut(s) 4
EcoICRI GAGCTC 1 cut(s) 154
EcoNI CCTNNNNNAGG 1 cut(s) 851
EcoO109I RGGNCCY 1 cut(s) 1090
EcoO65I GGTNACC 1 cut(s) 4
EcoRII CCWGG 2 cut(s) 1092, 1123
EcoT14I CCWWGG 2 cut(s) 333, 522
EcoT38I GRGCYC 3 cut(s) 156, 789, 1131
ErhI CCWWGG 2 cut(s) 333, 522
FaeI CATG 7 cut(s) 178, 211, 262, 412, 475, 820, 1012
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FaqI GGGAC 1 cut(s) 82
FatI CATG 7 cut(s) 174, 207, 258, 408, 471, 816, 1008
FauI CCCGC 1 cut(s) 690
FbaI TGATCA 1 cut(s) 192
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 5 cut(s) 327, 502, 657, 660, 726
FriOI GRGCYC 3 cut(s) 156, 789, 1131
Fsp4HI GCNGC 5 cut(s) 327, 502, 657, 660, 726
FspBI CTAG 1 cut(s) 603
GlaI GCGC 2 cut(s) 173, 467
GluI GCNGC 5 cut(s) 327, 502, 657, 660, 726
GsuI CTGGAG 2 cut(s) 1104, 1115
HaeII RGCGCY 1 cut(s) 469
HapII CCGG 5 cut(s) 47, 414, 498, 514, 911
HhaI GCGC 2 cut(s) 174, 468
Hin1II CATG 7 cut(s) 178, 211, 262, 412, 475, 820, 1012
Hin6I GCGC 2 cut(s) 172, 466
HinP1I GCGC 2 cut(s) 172, 466
HincII GTYRAC 3 cut(s) 13, 94, 406
HindII GTYRAC 3 cut(s) 13, 94, 406
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 1 cut(s) 238
HpaII CCGG 5 cut(s) 47, 414, 498, 514, 911
HphI GGTGA 7 cut(s) 16, 364, 424, 659, 788, 1057, 1123
Hpy166II GTNNAC 3 cut(s) 13, 94, 406
Hpy188I TCNGA 3 cut(s) 574, 691, 997
Hpy8I GTNNAC 3 cut(s) 13, 94, 406
Hpy99I CGWCG 2 cut(s) 1139, 1148
HpyAV CCTTC 4 cut(s) 276, 749, 758, 868
HpyCH4III ACNGT 8 cut(s) 10, 55, 508, 583, 653, 1135, 1144, 1157
HpyCH4IV ACGT 1 cut(s) 553
HpyCH4V TGCA 3 cut(s) 278, 905, 923
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 335, 436, 950
HpyF3I CTNAG 3 cut(s) 39, 534, 690
HpySE526I ACGT 1 cut(s) 553
Hsp92II CATG 7 cut(s) 178, 211, 262, 412, 475, 820, 1012
HspAI GCGC 2 cut(s) 172, 466
KroI GCCGGC 1 cut(s) 497
KroNI GCCGGC 1 cut(s) 499
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 4 cut(s) 192, 225, 576, 931
LmnI GCTCC 3 cut(s) 516, 548, 575
Lsp1109I GCAGC 2 cut(s) 313, 643
LweI GCATC 2 cut(s) 265, 778
MaeI CTAG 1 cut(s) 603
MaeII ACGT 1 cut(s) 553
MaeIII GTNAC 6 cut(s) 4, 179, 203, 586, 647, 1165
MalI GATC 4 cut(s) 194, 227, 578, 933
MboI GATC 4 cut(s) 192, 225, 576, 931
MboII GAAGA 3 cut(s) 233, 764, 841
MfeI CAATTG 1 cut(s) 85
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 7 cut(s) 144, 156, 513, 553, 789, 1074, 1131
MlsI TGGCCA 2 cut(s) 62, 521
MluCI AATT 6 cut(s) 85, 164, 303, 822, 924, 947
MluNI TGGCCA 2 cut(s) 62, 521
Mox20I TGGCCA 2 cut(s) 62, 521
MreI CGCCGGCG 1 cut(s) 497
MroNI GCCGGC 1 cut(s) 497
MroXI GAANNNNTTC 1 cut(s) 810
MscI TGGCCA 2 cut(s) 62, 521
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 2 cut(s) 774, 912
Msp20I TGGCCA 2 cut(s) 62, 521
MspA1I CMGCKG 1 cut(s) 685
MspI CCGG 5 cut(s) 47, 414, 498, 514, 911
MspR9I CCNGG 6 cut(s) 414, 415, 911, 912, 1094, 1125
MunI CAATTG 1 cut(s) 85
MvaI CCWGG 2 cut(s) 1094, 1125
MwoI GCNNNNNNNGC 4 cut(s) 35, 335, 436, 950
NaeI GCCGGC 1 cut(s) 499
NciI CCSGG 4 cut(s) 414, 415, 911, 912
NdeII GATC 4 cut(s) 192, 225, 576, 931
NgoMIV GCCGGC 1 cut(s) 497
NlaIII CATG 7 cut(s) 178, 211, 262, 412, 475, 820, 1012
NlaIV GGNNCC 5 cut(s) 45, 264, 418, 505, 757
NmeAIII GCCGAG 1 cut(s) 701
NmuCI GTSAC 5 cut(s) 4, 179, 586, 647, 1165
NspI RCATGY 4 cut(s) 178, 211, 412, 1012
NspV TTCGAA 1 cut(s) 827
OliI CACNNNNGTG 1 cut(s) 912
PagI TCATGA 1 cut(s) 471
PceI AGGCCT 2 cut(s) 447, 857
PciI ACATGT 2 cut(s) 207, 1008
PcsI WCGNNNNNNNCGW 2 cut(s) 550, 1143
PdiI GCCGGC 1 cut(s) 499
PdmI GAANNNNTTC 1 cut(s) 810
PfeI GAWTC 1 cut(s) 238
PflFI GACNNNGTC 1 cut(s) 17
PkrI GCNGC 5 cut(s) 328, 503, 658, 661, 727
PmaCI CACGTG 1 cut(s) 554
PmlI CACGTG 1 cut(s) 554
Ppu21I YACGTR 1 cut(s) 554
PscI ACATGT 2 cut(s) 207, 1008
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 1092, 1123
PspCI CACGTG 1 cut(s) 554
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 2 cut(s) 1092, 1123
PspN4I GGNNCC 5 cut(s) 45, 264, 418, 505, 757
PspPI GGNCC 8 cut(s) 44, 417, 599, 631, 756, 917, 1090, 1121
PsuI RGATCY 1 cut(s) 225
PsyI GACNNNGTC 1 cut(s) 17
RsaI GTAC 3 cut(s) 257, 391, 479
RsaNI GTAC 3 cut(s) 256, 390, 478
RseI CAYNNNNRTG 2 cut(s) 774, 912
SacI GAGCTC 1 cut(s) 156
SalI GTCGAC 1 cut(s) 92
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 5 cut(s) 327, 502, 657, 660, 726
Sau3AI GATC 4 cut(s) 192, 225, 576, 931
Sau96I GGNCC 8 cut(s) 44, 417, 599, 631, 756, 917, 1090, 1121
ScrFI CCNGG 6 cut(s) 414, 415, 911, 912, 1094, 1125
SduI GDGCHC 7 cut(s) 144, 156, 513, 553, 789, 1074, 1131
SfaNI GCATC 2 cut(s) 265, 778
SfcI CTRYAG 1 cut(s) 1059
SfuI TTCGAA 1 cut(s) 827
SgrAI CRCCGGYG 1 cut(s) 497
SinI GGWCC 1 cut(s) 44
SmaI CCCGGG 2 cut(s) 415, 912
SmiMI CAYNNNNRTG 2 cut(s) 774, 912
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 6 cut(s) 85, 164, 303, 822, 924, 947
SseBI AGGCCT 2 cut(s) 447, 857
SsiI CCGC 5 cut(s) 22, 501, 659, 683, 725
SspMI CTAG 1 cut(s) 603
SstI GAGCTC 1 cut(s) 156
StuI AGGCCT 2 cut(s) 447, 857
StyD4I CCNGG 6 cut(s) 412, 413, 909, 910, 1092, 1123
StyI CCWWGG 2 cut(s) 333, 522
TaaI ACNGT 8 cut(s) 10, 55, 508, 583, 653, 1135, 1144, 1157
TaiI ACGT 1 cut(s) 556
TaqI TCGA 6 cut(s) 93, 618, 804, 827, 837, 1137
TasI AATT 6 cut(s) 85, 164, 303, 822, 924, 947
TatI WGTACW 1 cut(s) 255
TauI GCSGC 3 cut(s) 504, 662, 728
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 6 cut(s) 403, 586, 1021, 1087, 1162, 1174
TseFI GTSAC 5 cut(s) 4, 179, 586, 647, 1165
TseI GCWGC 2 cut(s) 326, 656
Tsp45I GTSAC 5 cut(s) 4, 179, 586, 647, 1165
TspGWI ACGGA 3 cut(s) 448, 584, 893
TspMI CCCGGG 2 cut(s) 413, 910
TspRI CASTG 6 cut(s) 403, 586, 1021, 1087, 1162, 1174
Tth111I GACNNNGTC 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 44
XagI CCTNNNNNAGG 1 cut(s) 851
XapI RAATTY 2 cut(s) 164, 303
XceI RCATGY 4 cut(s) 178, 211, 412, 1012
XmaI CCCGGG 2 cut(s) 413, 910
XmiI GTMKAC 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.