AT5G39785

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002986
Physical Location & Seq
Forward (+)
15928765 .. 15932347
3583 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39785.3

Sequence Viewer

Length: 1821 bp
ATGATTGAATTAGAAAATTCTGGAATCGAAGATGAAGAAGAAGTTAGTGAATCGCCGAAATTGGTTTTTAAATTCGCGTTTCAGACGGAAACTTTCGAGAGATTAAGCAGAGAAGGTTATAGATCGTTGAGTGATGATTGCGGTGTGGAGAGCGAGAGCAGTTTTTTGTCTTCTGATTCTTCTCCGGTGATTTTTACGGCGGCGGCGAGGAAATACGAGACACCGTCGGCGAAAAATCTCACCTTTGTCCTCGAAAACCCAAAGGCGGCGACTTTTAGGGTTGAAGATTGTTTGAAGGAGAAGCAAGAGAAAACAGAGGATTTAGGTTACTCTGTTTTTCACGGCGAGGATAAAGTAAAAACAGAGGATTACTCTGTTTCATCCTTTAAGAAGAAAAAAATCAGATTTTTAACAGAGGAAGATTTTTTGGAGTCGGACTCTGATTTTGTTGATTCAAGTCAGACATTCACAAGCAACGACGAAGATGGGTTTTTATCAGATTCAGATTTCGCAGAAACATCACTGAAGAAAGGACAAAACCGTAAAAGCGACAATTCTGGTTCTGGTTCTGATTCAGAGGAAGAAGAAGAAGAAGATACAAATGGGTTTGAGAGTTTATGGGAGCATCAAGATTTGATAGAACAACTTAAGATGGAGATGAAGAAAGTTAAAGCAATTGGTGGGCTTACGACAATTCTTGAAGAAGAAGAAGAAGATGATGATTGTCCTAAGATCATGGAAGATTTAAAACCATGGAGGATTGAAGAAGAGAAGAAATTTAAACATGTTGATACGATTGGTGAAGTTCATAAGTTCCATAGAAGTTACAGAGAAAGAATGCGAAAGCTTGATATCTTGAGTTTTCAAAAGTCTTATGCTCTTGGGCTTCTTCAATCAAAGAGCCCTCAACAAGCAACCTCGACCCTTGGATCCAATCCTTCGCAGACATCGTTTTCGTCGGTTTTCTCGGTTAATATCCGGTTATGGAAAGCGAAGAAATCTGAAATCGAACCAATGGTTCAATTTGTAAAAGAGATTCAAGGGGAATTAGAGAACGTATACGTAGGTCAAATGTGTTTGTCGTGGGAAATACTTCACTGGCAATACGAAAAGGCCATTGAGTTACTAGAATCTGATGTGTATGGATCTCGTCGGTACAACGAAGTCGCCGGAGAGTTTCAACAGTTTCAGGTTTTGCTTCAAAGGTTTCTTGAAAACGAGCCATTCGAAGAGCCTAGAGTACAACATTACATCAAGAGACGTTGTGTTCTTCGTAATCTCCTTCAGATCCCGGTTATTCGAGAAGATGGAAACAAGGACAAAAAGAATGGCAGAAGAAGAGATTACGAGGAAAATAATGACGGTGTGATTAAAAGCGATCAACTAGTGGAAATAATGGAGGAAACAATTCGATTATTTTGGCGATTTGTTCGGTGTGATAAGCTTACTAGCTCGATACATGATCAGAAATCGCGAACCAAATCACAAATAGAACCGGATCATGAAGAAGACTCGGAAGACCTAGAGATGTTCGCAGAGGTTAAATCACAACTTCAAAATAAGGAGAAAAGGTTGAGAGATGTGTTGAAAAGTGAGAGATGTATTATAAGAAGATTCCAAAAGCACAAGGAAGAAGATTCGACAGAAGATCAAGTACTTCACTTCTTCTCACAAGTAGACATGAAACTTGTGACAAGAGTCTTGAATATGTCTAAATTGACTAGGGACCATTTGGTTTGGTGTCACAATAAGTTGACTAAGATTAACTTCGTAAATAGAAGGTTACATTTAGATCCTTCCTTTTGTCTTTTCCCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

606

Amino Acids

71.04

Weight (kDa)

5.08

Isoelectric Point (pI)

62.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 349 - 605 1.1e-93 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1607
AccI GTMKAC 2 cut(s) 1059, 1677
AccII CGCG 2 cut(s) 77, 1474
AciI CCGC 4 cut(s) 141, 200, 203, 266
AclWI GGATC 6 cut(s) 924, 937, 1153, 1282, 1506, 1787
AcsI RAATTY 3 cut(s) 16, 71, 776
AcuI CTGAAG 2 cut(s) 545, 1268
AfaI GTAC 3 cut(s) 1157, 1242, 1656
AfiI CCNNNNNNNGG 1 cut(s) 265
AflII CTTAAG 1 cut(s) 647
AflIII ACRYGT 1 cut(s) 784
AhlI ACTAGT 1 cut(s) 1384
AluBI AGCT 3 cut(s) 847, 1444, 1452
AluI AGCT 3 cut(s) 847, 1444, 1452
Alw26I GTCTC 2 cut(s) 212, 1252
AlwI GGATC 6 cut(s) 924, 937, 1153, 1282, 1506, 1787
AoxI GGCC 1 cut(s) 1113
ApoI RAATTY 3 cut(s) 16, 71, 776
ArsI GACNNNNNNTTYG 4 cut(s) 428, 460, 937, 969
Asp700I GAANNNNTTC 2 cut(s) 1092, 1407
AspS9I GGNCC 1 cut(s) 1726
AsuC2I CCSGG 1 cut(s) 1292
AsuHPI GGTGA 3 cut(s) 199, 232, 812
AsuII TTCGAA 1 cut(s) 1227
AvaII GGWCC 1 cut(s) 1726
BamHI GGATCC 1 cut(s) 929
BanII GRGCYC 1 cut(s) 905
BarI GAAGNNNNNNTAC 2 cut(s) 1638, 1670
BbsI GAAGAC 3 cut(s) 162, 1515, 1524
BccI CCATC 3 cut(s) 479, 646, 1301
BceAI ACGGC 2 cut(s) 213, 358
BclI TGATCA 1 cut(s) 1462
BcnI CCSGG 1 cut(s) 1292
BcoDI GTCTC 2 cut(s) 212, 1252
BcuI ACTAGT 1 cut(s) 1384
BfaI CTAG 6 cut(s) 1127, 1236, 1385, 1449, 1523, 1722
BfrI CTTAAG 1 cut(s) 647
BisI GCNGC 3 cut(s) 201, 204, 267
BlsI GCNGC 3 cut(s) 202, 205, 268
BmcAI AGTACT 1 cut(s) 1656
Bme1390I CCNGG 1 cut(s) 1292
Bme18I GGWCC 1 cut(s) 1726
BmgT120I GGNCC 1 cut(s) 1726
BmiI GGNNCC 2 cut(s) 931, 1727
BmrFI CCNGG 1 cut(s) 1292
BmsI GCATC 1 cut(s) 634
BoxI GACNNNNGTC 1 cut(s) 1697
BpiI GAAGAC 3 cut(s) 162, 1515, 1524
BplI GAGNNNNNCTC 4 cut(s) 356, 388, 422, 454
Bpu14I TTCGAA 1 cut(s) 1227
BpuEI CTTGAG 1 cut(s) 877
BpuMI CCSGG 1 cut(s) 1292
BsaAI YACGTR 1 cut(s) 1063
BsaJI CCNNGG 2 cut(s) 752, 925
BsaWI WCCGGW 3 cut(s) 184, 978, 1495
BsaXI ACNNNNNCTCC 2 cut(s) 1556, 1586
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 1103
BseDI CCNNGG 2 cut(s) 752, 925
BseGI GGATG 1 cut(s) 380
BseLI CCNNNNNNNGG 1 cut(s) 265
BseNI ACTGG 1 cut(s) 1103
Bsh1236I CGCG 2 cut(s) 77, 1474
BshFI GGCC 1 cut(s) 1115
BsiSI CCGG 5 cut(s) 185, 979, 1170, 1292, 1496
BslFI GGGAC 1 cut(s) 1739
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 2 cut(s) 212, 1252
BsmBI CGTCTC 1 cut(s) 1252
BsmFI GGGAC 1 cut(s) 1739
BsmI GAATGC 1 cut(s) 843
BsnI GGCC 1 cut(s) 1115
Bsp119I TTCGAA 1 cut(s) 1227
Bsp1286I GDGCHC 1 cut(s) 905
Bsp19I CCATGG 1 cut(s) 752
Bsp68I TCGCGA 1 cut(s) 1474
BspACI CCGC 4 cut(s) 141, 200, 203, 266
BspANI GGCC 1 cut(s) 1115
BspFNI CGCG 2 cut(s) 77, 1474
BspHI TCATGA 1 cut(s) 1501
BspLI GGNNCC 2 cut(s) 931, 1727
BspPI GGATC 6 cut(s) 924, 937, 1153, 1282, 1506, 1787
BspQI GCTCTTC 1 cut(s) 1224
BspT104I TTCGAA 1 cut(s) 1227
BspTI CTTAAG 1 cut(s) 647
BsrI ACTGG 1 cut(s) 1103
BssECI CCNNGG 2 cut(s) 752, 925
BssNAI GTATAC 1 cut(s) 1060
BssT1I CCWWGG 2 cut(s) 752, 925
Bst1107I GTATAC 1 cut(s) 1060
Bst4CI ACNGT 4 cut(s) 225, 542, 1185, 1364
Bst6I CTCTTC 3 cut(s) 762, 1224, 1333
BstAFI CTTAAG 1 cut(s) 647
BstBAI YACGTR 1 cut(s) 1063
BstBI TTCGAA 1 cut(s) 1227
BstDEI CTNAG 2 cut(s) 729, 1758
BstDSI CCRYGG 1 cut(s) 752
BstF5I GGATG 1 cut(s) 380
BstFNI CGCG 2 cut(s) 77, 1474
BstMAI GTCTC 2 cut(s) 212, 1252
BstNSI RCATGY 1 cut(s) 788
BstPAI GACNNNNGTC 1 cut(s) 1697
BstSCI CCNGG 1 cut(s) 1290
BstSNI TACGTA 1 cut(s) 1063
BstUI CGCG 2 cut(s) 77, 1474
BstV2I GAAGAC 3 cut(s) 162, 1515, 1524
BstX2I RGATCY 4 cut(s) 929, 1145, 1287, 1792
BstYI RGATCY 4 cut(s) 929, 1145, 1287, 1792
BstZ17I GTATAC 1 cut(s) 1060
BsuRI GGCC 1 cut(s) 1115
BtgI CCRYGG 1 cut(s) 752
BtsCI GGATG 1 cut(s) 380
BtsIMutI CAGTG 2 cut(s) 521, 1096
BtuMI TCGCGA 1 cut(s) 1474
CciI TCATGA 1 cut(s) 1501
Cfr13I GGNCC 1 cut(s) 1726
Csp6I GTAC 3 cut(s) 1156, 1241, 1655
CviAII CATG 6 cut(s) 736, 753, 785, 1460, 1502, 1681
CviJI RGCY 9 cut(s) 685, 847, 886, 903, 1115, 1222, 1234, 1444, 1452
CviKI_1 RGCY 9 cut(s) 685, 847, 886, 903, 1115, 1222, 1234, 1444, 1452
CviQI GTAC 3 cut(s) 1156, 1241, 1655
DdeI CTNAG 2 cut(s) 729, 1758
DraI TTTAAA 3 cut(s) 70, 747, 781
Eam1104I CTCTTC 3 cut(s) 762, 1224, 1333
EarI CTCTTC 3 cut(s) 762, 1224, 1333
Eco105I TACGTA 1 cut(s) 1063
Eco130I CCWWGG 2 cut(s) 752, 925
Eco24I GRGCYC 1 cut(s) 905
Eco32I GATATC 1 cut(s) 853
Eco47I GGWCC 1 cut(s) 1726
Eco57I CTGAAG 2 cut(s) 545, 1268
EcoRV GATATC 1 cut(s) 853
EcoT14I CCWWGG 2 cut(s) 752, 925
EcoT38I GRGCYC 1 cut(s) 905
ErhI CCWWGG 2 cut(s) 752, 925
Esp3I CGTCTC 1 cut(s) 1252
FaeI CATG 6 cut(s) 739, 756, 788, 1463, 1505, 1684
FalI AAGNNNNNCTT 2 cut(s) 1751, 1783
FaqI GGGAC 1 cut(s) 1739
FatI CATG 6 cut(s) 735, 752, 784, 1459, 1501, 1680
FbaI TGATCA 1 cut(s) 1462
FblI GTMKAC 2 cut(s) 1059, 1677
Fnu4HI GCNGC 3 cut(s) 201, 204, 267
FokI GGATG 1 cut(s) 367
FriOI GRGCYC 1 cut(s) 905
Fsp4HI GCNGC 3 cut(s) 201, 204, 267
FspBI CTAG 6 cut(s) 1127, 1236, 1385, 1449, 1523, 1722
GluI GCNGC 3 cut(s) 201, 204, 267
HaeIII GGCC 1 cut(s) 1115
HapII CCGG 5 cut(s) 185, 979, 1170, 1292, 1496
Hin1II CATG 6 cut(s) 739, 756, 788, 1463, 1505, 1684
HincII GTYRAC 1 cut(s) 1755
HindII GTYRAC 1 cut(s) 1755
HindIII AAGCTT 2 cut(s) 845, 1442
HpaII CCGG 5 cut(s) 185, 979, 1170, 1292, 1496
HphI GGTGA 3 cut(s) 199, 232, 812
Hpy166II GTNNAC 3 cut(s) 1060, 1678, 1755
Hpy8I GTNNAC 3 cut(s) 1060, 1678, 1755
Hpy99I CGWCG 4 cut(s) 229, 482, 961, 1155
HpyAV CCTTC 6 cut(s) 107, 289, 948, 1292, 1773, 1806
HpyCH4III ACNGT 4 cut(s) 225, 542, 1185, 1364
HpyCH4IV ACGT 3 cut(s) 1056, 1062, 1261
HpyF3I CTNAG 2 cut(s) 729, 1758
HpySE526I ACGT 3 cut(s) 1056, 1062, 1261
Hsp92II CATG 6 cut(s) 739, 756, 788, 1463, 1505, 1684
Ksp22I TGATCA 1 cut(s) 1462
LguI GCTCTTC 1 cut(s) 1224
LmnI GCTCC 1 cut(s) 622
LweI GCATC 1 cut(s) 634
MaeI CTAG 6 cut(s) 1127, 1236, 1385, 1449, 1523, 1722
MaeII ACGT 3 cut(s) 1056, 1062, 1261
MaeIII GTNAC 6 cut(s) 326, 824, 1122, 1690, 1742, 1782
MfeI CAATTG 1 cut(s) 675
MflI RGATCY 4 cut(s) 929, 1145, 1287, 1792
MhlI GDGCHC 1 cut(s) 905
MlyI GAGTC 4 cut(s) 431, 440, 1505, 1707
MmeI TCCRAC 1 cut(s) 414
MroXI GAANNNNTTC 2 cut(s) 1092, 1407
MspCI CTTAAG 1 cut(s) 647
MspI CCGG 5 cut(s) 185, 979, 1170, 1292, 1496
MspR9I CCNGG 1 cut(s) 1292
MunI CAATTG 1 cut(s) 675
Mva1269I GAATGC 1 cut(s) 843
MvnI CGCG 2 cut(s) 77, 1474
NciI CCSGG 1 cut(s) 1292
NcoI CCATGG 1 cut(s) 752
NlaIII CATG 6 cut(s) 739, 756, 788, 1463, 1505, 1684
NlaIV GGNNCC 2 cut(s) 931, 1727
NmuCI GTSAC 2 cut(s) 1690, 1742
NruI TCGCGA 1 cut(s) 1474
NspI RCATGY 1 cut(s) 788
NspV TTCGAA 1 cut(s) 1227
PagI TCATGA 1 cut(s) 1501
PciI ACATGT 1 cut(s) 784
PciSI GCTCTTC 1 cut(s) 1224
PcsI WCGNNNNNNNCGW 3 cut(s) 203, 947, 1224
PctI GAATGC 1 cut(s) 843
PdmI GAANNNNTTC 2 cut(s) 1092, 1407
PflFI GACNNNGTC 1 cut(s) 223
PkrI GCNGC 3 cut(s) 202, 205, 268
PleI GAGTC 4 cut(s) 431, 439, 1505, 1706
PpsI GAGTC 4 cut(s) 431, 439, 1505, 1706
Ppu21I YACGTR 1 cut(s) 1063
PscI ACATGT 1 cut(s) 784
PshAI GACNNNNGTC 1 cut(s) 1697
PsiI TTATAA 1 cut(s) 1607
PspN4I GGNNCC 2 cut(s) 931, 1727
PspPI GGNCC 1 cut(s) 1726
PsuI RGATCY 4 cut(s) 929, 1145, 1287, 1792
PsyI GACNNNGTC 1 cut(s) 223
RruI TCGCGA 1 cut(s) 1474
RsaI GTAC 3 cut(s) 1157, 1242, 1656
RsaNI GTAC 3 cut(s) 1156, 1241, 1655
SapI GCTCTTC 1 cut(s) 1224
SatI GCNGC 3 cut(s) 201, 204, 267
Sau96I GGNCC 1 cut(s) 1726
ScaI AGTACT 1 cut(s) 1656
SchI GAGTC 4 cut(s) 431, 440, 1505, 1707
ScrFI CCNGG 1 cut(s) 1292
SduI GDGCHC 1 cut(s) 905
SfaNI GCATC 1 cut(s) 634
SfuI TTCGAA 1 cut(s) 1227
SinI GGWCC 1 cut(s) 1726
SmlI CTYRAG 2 cut(s) 647, 856
SmoI CTYRAG 2 cut(s) 647, 856
SnaBI TACGTA 1 cut(s) 1063
SpeI ACTAGT 1 cut(s) 1384
SsiI CCGC 4 cut(s) 141, 200, 203, 266
SspMI CTAG 6 cut(s) 1127, 1236, 1385, 1449, 1523, 1722
StyD4I CCNGG 1 cut(s) 1290
StyI CCWWGG 2 cut(s) 752, 925
TaaI ACNGT 4 cut(s) 225, 542, 1185, 1364
TaiI ACGT 3 cut(s) 1059, 1065, 1264
TatI WGTACW 2 cut(s) 1240, 1654
TauI GCSGC 3 cut(s) 203, 206, 269
TscAI CASTG 2 cut(s) 528, 1103
TseFI GTSAC 2 cut(s) 1690, 1742
Tsp45I GTSAC 2 cut(s) 1690, 1742
TspDTI ATGAA 6 cut(s) 48, 369, 674, 797, 1518, 1697
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 2 cut(s) 528, 1103
Tth111I GACNNNGTC 1 cut(s) 223
Vha464I CTTAAG 1 cut(s) 647
VpaK11BI GGWCC 1 cut(s) 1726
XapI RAATTY 3 cut(s) 16, 71, 776
XceI RCATGY 1 cut(s) 788
XmiI GTMKAC 2 cut(s) 1059, 1677
XmnI GAANNNNTTC 2 cut(s) 1092, 1407
XspI CTAG 6 cut(s) 1127, 1236, 1385, 1449, 1523, 1722
ZrmI AGTACT 1 cut(s) 1656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.