RLG00000021786

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002986
Physical Location & Seq
Forward (+)
78303946 .. 78307361
3416 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021786

Sequence Viewer

Length: 1458 bp
ATGAGTGGTTCTTCTGATTGGATTTTCAAAAAAAAGATGTCTCCTCTTTCTGGGTCCTTCTCGGTTTTTGTCTCATCCATTTTGCACACTCTGTTCAGATTTGTTAGCTCAATCTTTGTCAGGTTTGATAAAGAACAGAGTTCTCTGGTGAATGAGTCTTGTTCTTCAATTGAGCCAGCAGCTATGCCCCAAAAAGTTGCGTCTGAGGTTTCAAAAAGCGATGATGATTCGGAGTACTATGATGTTGAAGATGAAAAAGAGACGCCGGAGCCAAGTTTTGTGTTCAAATTTGAGTATCAGACTAAAGCCGTATTAGAAGCAATGGAGAAAGTGAATGCTTATGGTCATTCTTCTTTTGGAACCAACAAAGAGAAATCCGAGTCTCCGAATATGGAGGATTTGAAGCCATGGAAGATTGATGAAAAATACCATCATGTAGACAGAATGAGTGTGCTTCACAAGTTCTACAAGACCTACAGAGAACGAATGCGAAAATTCGACATTTTGAGTTACCAGAAGTTATATGCAATAGGATTTTTGCAGTCCAAGGACACGTTCGAAACCTTTTCAAGCTGCAAATCCTCAACTCCAGCAATCAAATCCTTTCTTTTGCAGAACTTTGGGCTATGCAAACCGAAGAAAAATTTTAGTTCTGATTCTGACCCAATGGTTAAGTTCATTAGAGAATTGCATAGTGAATTGGAAGTGGTTTATGTTGGACAGTTGTGCCTTTCTTGGGAATTCCTTCAATGGCAATATGAGAAGGCATTGGAGCTTTGGGAATATGACGAATATGGATTACGTTCGTATAATGAAGTTGCCGAGGAATTTCAACAATTTCAAGTGCTCATGCAAAGATTTGTGGAGAATGAACGCTTTCAAGGGCCAAGGGTTGAGACCTATGTCAAAAATCGATGTGTTATGCGGAATCTTATGCAAATTCCAGTGATTAGGGAGGACAGTATGAAGGAAAAAAAGAAAGCAAAGAGAAAAGGCAAAGACAATGATGATGCAGTTGGAAGTGATATTCTAGTAGAGATATTGCAGGAATCAATAAGGACCATTTGGCGATTCATACAAGCTGATAAATCTGCTAATACAACGGATGCTTGTCGAAAAAGATTAAAGTTGGAAGTTGAAGACTCCTTGGATCCAAAGCTATTAGTGGAGGTTCAAGCAGAGCTTCAAAAGAAAGAAAAGAAGCTTAAAGAGCTGTACAGGAGTGAGAACTGCATACTAAAGAGATTCAAAAAGCACCATGAAGAAGAAGAGGAAGAAGAGAGCGCAGAACACCTCTACTTCTTTTGTAAAGTTGACATCAAATTAGTGTCGAGGGTTTTAAACATGTCAAGCGTAACAACTGATCAACTAGTTTGGTGTCACAATAAGCTAAGCAGGATACATATTGTGAACCGAAAGATTCGTGTAGATCCTTCTTTCTTACTATTTCCATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

486

Amino Acids

57.16

Weight (kDa)

6.64

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 232 - 484 1e-93 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 438
AciI CCGC 1 cut(s) 925
AclWI GGATC 3 cut(s) 1145, 1158, 1424
AcsI RAATTY 6 cut(s) 287, 494, 643, 740, 827, 939
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 2 cut(s) 236, 1217
AfiI CCNNNNNNNGG 2 cut(s) 50, 736
AflIII ACRYGT 2 cut(s) 552, 1344
AhlI ACTAGT 1 cut(s) 1369
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
Alw21I GWGCWC 1 cut(s) 849
Alw26I GTCTC 5 cut(s) 45, 76, 254, 387, 890
AlwI GGATC 3 cut(s) 1145, 1158, 1424
AoxI GGCC 1 cut(s) 884
ApeKI GCWGC 2 cut(s) 179, 573
ApoI RAATTY 6 cut(s) 287, 494, 643, 740, 827, 939
Asp700I GAANNNNTTC 2 cut(s) 744, 876
AspLEI GCGC 1 cut(s) 1286
AspS9I GGNCC 3 cut(s) 54, 884, 1059
AsuHPI GGTGA 1 cut(s) 160
AsuII TTCGAA 1 cut(s) 558
AvaII GGWCC 2 cut(s) 54, 1059
BamHI GGATCC 1 cut(s) 1150
BbsI GAAGAC 1 cut(s) 1146
Bbv12I GWGCWC 1 cut(s) 849
BbvI GCAGC 2 cut(s) 191, 560
BccI CCATC 1 cut(s) 438
BceAI ACGGC 1 cut(s) 293
BciVI GTATCC 1 cut(s) 1392
BclI TGATCA 1 cut(s) 1363
BcoDI GTCTC 5 cut(s) 45, 76, 254, 387, 890
BcuI ACTAGT 1 cut(s) 1369
BfaI CTAG 2 cut(s) 1031, 1370
BfmI CTRYAG 1 cut(s) 475
BfuI GTATCC 1 cut(s) 1392
BisI GCNGC 2 cut(s) 180, 574
BlpI GCTNAGC 1 cut(s) 1391
BlsI GCNGC 2 cut(s) 181, 575
BmcAI AGTACT 1 cut(s) 236
Bme18I GGWCC 2 cut(s) 54, 1059
BmgT120I GGNCC 3 cut(s) 54, 884, 1059
BmiI GGNNCC 4 cut(s) 55, 270, 361, 1152
BmsI GCATC 2 cut(s) 1000, 1096
BoxI GACNNNNGTC 1 cut(s) 902
BpiI GAAGAC 1 cut(s) 1146
BpmI CTGGAG 1 cut(s) 573
Bpu1102I GCTNAGC 1 cut(s) 1391
Bpu14I TTCGAA 1 cut(s) 558
Bsa29I ATCGAT 1 cut(s) 913
BsaHI GRCGYC 1 cut(s) 263
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 5 cut(s) 407, 546, 822, 887, 1146
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 736
Bse1I ACTGG 1 cut(s) 944
Bse3DI GCAATG 1 cut(s) 327
BseCI ATCGAT 1 cut(s) 913
BseDI CCNNGG 5 cut(s) 407, 546, 822, 887, 1146
BseGI GGATG 2 cut(s) 74, 1111
BseLI CCNNNNNNNGG 2 cut(s) 50, 736
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 944
BseRI GAGGAG 1 cut(s) 33
BseXI GCAGC 2 cut(s) 191, 560
BshFI GGCC 1 cut(s) 886
BshVI ATCGAT 1 cut(s) 913
BsiHKAI GWGCWC 1 cut(s) 849
BsiSI CCGG 1 cut(s) 266
BslI CCNNNNNNNGG 2 cut(s) 50, 736
BsmAI GTCTC 5 cut(s) 45, 76, 254, 387, 890
BsmBI CGTCTC 1 cut(s) 254
BsmI GAATGC 2 cut(s) 340, 492
BsnI GGCC 1 cut(s) 886
Bso31I GGTCTC 1 cut(s) 890
Bsp119I TTCGAA 1 cut(s) 558
Bsp1286I GDGCHC 1 cut(s) 849
Bsp1407I TGTACA 1 cut(s) 1215
Bsp143I GATC 3 cut(s) 1150, 1363, 1429
Bsp1720I GCTNAGC 1 cut(s) 1391
Bsp19I CCATGG 1 cut(s) 407
BspACI CCGC 1 cut(s) 925
BspANI GGCC 1 cut(s) 886
BspCNI CTCAG 1 cut(s) 196
BspDI ATCGAT 1 cut(s) 913
BspLI GGNNCC 4 cut(s) 55, 270, 361, 1152
BspPI GGATC 3 cut(s) 1145, 1158, 1424
BspT104I TTCGAA 1 cut(s) 558
BspTNI GGTCTC 1 cut(s) 890
BsrDI GCAATG 1 cut(s) 327
BsrGI TGTACA 1 cut(s) 1215
BsrI ACTGG 1 cut(s) 944
BssECI CCNNGG 5 cut(s) 407, 546, 822, 887, 1146
BssMI GATC 3 cut(s) 1150, 1363, 1429
BssNI GRCGYC 1 cut(s) 263
BssT1I CCWWGG 4 cut(s) 407, 546, 887, 1146
Bst4CI ACNGT 2 cut(s) 723, 962
Bst6I CTCTTC 2 cut(s) 1263, 1272
BstACI GRCGYC 1 cut(s) 263
BstAUI TGTACA 1 cut(s) 1215
BstBI TTCGAA 1 cut(s) 558
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 2 cut(s) 204, 1391
BstDSI CCRYGG 1 cut(s) 407
BstF5I GGATG 2 cut(s) 74, 1111
BstHHI GCGC 1 cut(s) 1286
BstKTI GATC 3 cut(s) 1153, 1366, 1432
BstMAI GTCTC 5 cut(s) 45, 76, 254, 387, 890
BstMBI GATC 3 cut(s) 1150, 1363, 1429
BstMWI GCNNNNNNNGC 1 cut(s) 1210
BstNSI RCATGY 1 cut(s) 1348
BstPAI GACNNNNGTC 1 cut(s) 902
BstSFI CTRYAG 1 cut(s) 475
BstV1I GCAGC 2 cut(s) 191, 560
BstV2I GAAGAC 1 cut(s) 1146
BstX2I RGATCY 2 cut(s) 1150, 1429
BstYI RGATCY 2 cut(s) 1150, 1429
Bsu15I ATCGAT 1 cut(s) 913
BsuI GTATCC 1 cut(s) 1392
BsuRI GGCC 1 cut(s) 886
BsuTUI ATCGAT 1 cut(s) 913
BtgI CCRYGG 1 cut(s) 407
BtgZI GCGATG 1 cut(s) 234
BtsCI GGATG 2 cut(s) 74, 1111
BtsIMutI CAGTG 1 cut(s) 951
Cac8I GCNNGC 1 cut(s) 177
CfoI GCGC 1 cut(s) 1286
Cfr13I GGNCC 3 cut(s) 54, 884, 1059
ClaI ATCGAT 1 cut(s) 913
CseI GACGC 2 cut(s) 189, 271
Csp6I GTAC 2 cut(s) 235, 1216
CviAII CATG 6 cut(s) 408, 434, 850, 1259, 1345, 1452
CviQI GTAC 2 cut(s) 235, 1216
DdeI CTNAG 2 cut(s) 204, 1391
DpnI GATC 3 cut(s) 1152, 1365, 1431
DpnII GATC 3 cut(s) 1150, 1363, 1429
DraI TTTAAA 1 cut(s) 1341
Eam1104I CTCTTC 2 cut(s) 1263, 1272
EarI CTCTTC 2 cut(s) 1263, 1272
Eco130I CCWWGG 4 cut(s) 407, 546, 887, 1146
Eco31I GGTCTC 1 cut(s) 890
Eco47I GGWCC 2 cut(s) 54, 1059
EcoO109I RGGNCCY 1 cut(s) 54
EcoRI GAATTC 1 cut(s) 740
EcoT14I CCWWGG 4 cut(s) 407, 546, 887, 1146
ErhI CCWWGG 4 cut(s) 407, 546, 887, 1146
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 6 cut(s) 411, 437, 853, 1262, 1348, 1455
FalI AAGNNNNNCTT 2 cut(s) 1167, 1199
FatI CATG 6 cut(s) 407, 433, 849, 1258, 1344, 1451
FbaI TGATCA 1 cut(s) 1363
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 2 cut(s) 180, 574
FokI GGATG 2 cut(s) 61, 1118
Fsp4HI GCNGC 2 cut(s) 180, 574
FspBI CTAG 2 cut(s) 1031, 1370
GlaI GCGC 1 cut(s) 1285
GluI GCNGC 2 cut(s) 180, 574
GsuI CTGGAG 1 cut(s) 573
HaeIII GGCC 1 cut(s) 886
HapII CCGG 1 cut(s) 266
HgaI GACGC 2 cut(s) 189, 271
HhaI GCGC 1 cut(s) 1286
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 6 cut(s) 411, 437, 853, 1262, 1348, 1455
Hin6I GCGC 1 cut(s) 1284
HinP1I GCGC 1 cut(s) 1284
HincII GTYRAC 1 cut(s) 1315
HindII GTYRAC 1 cut(s) 1315
HindIII AAGCTT 1 cut(s) 1202
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 3 cut(s) 439, 1315, 1411
Hpy188I TCNGA 9 cut(s) 16, 98, 205, 232, 300, 379, 387, 655, 661
Hpy8I GTNNAC 3 cut(s) 439, 1315, 1411
HpyAV CCTTC 5 cut(s) 67, 755, 757, 961, 1443
HpyCH4III ACNGT 2 cut(s) 723, 962
HpyCH4IV ACGT 2 cut(s) 554, 802
HpyF10VI GCNNNNNNNGC 1 cut(s) 1210
HpyF3I CTNAG 2 cut(s) 204, 1391
HpySE526I ACGT 2 cut(s) 554, 802
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 6 cut(s) 411, 437, 853, 1262, 1348, 1455
HspAI GCGC 1 cut(s) 1284
Ksp22I TGATCA 1 cut(s) 1363
Kzo9I GATC 3 cut(s) 1150, 1363, 1429
LmnI GCTCC 2 cut(s) 268, 772
Lsp1109I GCAGC 2 cut(s) 191, 560
LweI GCATC 2 cut(s) 1000, 1096
MaeI CTAG 2 cut(s) 1031, 1370
MaeII ACGT 2 cut(s) 554, 802
MaeIII GTNAC 3 cut(s) 509, 1354, 1379
MalI GATC 3 cut(s) 1152, 1365, 1431
MboI GATC 3 cut(s) 1150, 1363, 1429
MfeI CAATTG 1 cut(s) 168
MflI RGATCY 2 cut(s) 1150, 1429
MhlI GDGCHC 1 cut(s) 849
MlyI GAGTC 3 cut(s) 164, 389, 1136
MmeI TCCRAC 3 cut(s) 697, 997, 1110
MroXI GAANNNNTTC 2 cut(s) 744, 876
MseI TTAA 4 cut(s) 672, 1124, 1206, 1340
MspI CCGG 1 cut(s) 266
MunI CAATTG 1 cut(s) 168
Mva1269I GAATGC 2 cut(s) 340, 492
MwoI GCNNNNNNNGC 1 cut(s) 1210
NcoI CCATGG 1 cut(s) 407
NdeII GATC 3 cut(s) 1150, 1363, 1429
NlaIII CATG 6 cut(s) 411, 437, 853, 1262, 1348, 1455
NlaIV GGNNCC 4 cut(s) 55, 270, 361, 1152
NmeAIII GCCGAG 1 cut(s) 847
NmuCI GTSAC 1 cut(s) 1379
NspI RCATGY 1 cut(s) 1348
NspV TTCGAA 1 cut(s) 558
PciI ACATGT 1 cut(s) 1344
PctI GAATGC 2 cut(s) 340, 492
PdmI GAANNNNTTC 2 cut(s) 744, 876
PfeI GAWTC 7 cut(s) 227, 656, 928, 1049, 1071, 1245, 1420
PkrI GCNGC 2 cut(s) 181, 575
PleI GAGTC 3 cut(s) 163, 388, 1136
PpsI GAGTC 3 cut(s) 163, 388, 1136
PpuMI RGGWCCY 1 cut(s) 54
PscI ACATGT 1 cut(s) 1344
PshAI GACNNNNGTC 1 cut(s) 902
Psp5II RGGWCCY 1 cut(s) 54
PspN4I GGNNCC 4 cut(s) 55, 270, 361, 1152
PspPI GGNCC 3 cut(s) 54, 884, 1059
PspPPI RGGWCCY 1 cut(s) 54
PsuI RGATCY 2 cut(s) 1150, 1429
RsaI GTAC 2 cut(s) 236, 1217
RsaNI GTAC 2 cut(s) 235, 1216
SaqAI TTAA 4 cut(s) 672, 1124, 1206, 1340
SatI GCNGC 2 cut(s) 180, 574
Sau3AI GATC 3 cut(s) 1150, 1363, 1429
Sau96I GGNCC 3 cut(s) 54, 884, 1059
ScaI AGTACT 1 cut(s) 236
SchI GAGTC 3 cut(s) 164, 389, 1136
SduI GDGCHC 1 cut(s) 849
SfaNI GCATC 2 cut(s) 1000, 1096
SfcI CTRYAG 1 cut(s) 475
SfuI TTCGAA 1 cut(s) 558
SinI GGWCC 2 cut(s) 54, 1059
SpeI ACTAGT 1 cut(s) 1369
SsiI CCGC 1 cut(s) 925
SspMI CTAG 2 cut(s) 1031, 1370
StyI CCWWGG 4 cut(s) 407, 546, 887, 1146
TaaI ACNGT 2 cut(s) 723, 962
TaiI ACGT 2 cut(s) 557, 805
TaqI TCGA 5 cut(s) 498, 558, 913, 1114, 1331
TatI WGTACW 2 cut(s) 234, 1215
TfiI GAWTC 7 cut(s) 227, 656, 928, 1049, 1071, 1245, 1420
Tru1I TTAA 4 cut(s) 672, 1124, 1206, 1340
Tru9I TTAA 4 cut(s) 672, 1124, 1206, 1340
TscAI CASTG 1 cut(s) 951
TseFI GTSAC 1 cut(s) 1379
TseI GCWGC 2 cut(s) 179, 573
Tsp45I GTSAC 1 cut(s) 1379
TspDTI ATGAA 8 cut(s) 267, 435, 667, 828, 885, 980, 1063, 1275
TspGWI ACGGA 1 cut(s) 1118
TspRI CASTG 1 cut(s) 951
VpaK11BI GGWCC 2 cut(s) 54, 1059
XapI RAATTY 6 cut(s) 287, 494, 643, 740, 827, 939
XceI RCATGY 1 cut(s) 1348
XmiI GTMKAC 1 cut(s) 438
XmnI GAANNNNTTC 2 cut(s) 744, 876
XspI CTAG 2 cut(s) 1031, 1370
ZrmI AGTACT 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.