Rw2G050110

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002986
Physical Location & Seq
Forward (+)
77759553 .. 77763305
3753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050110.1

Sequence Viewer

Length: 2220 bp
ATGCCCCAAAAAGTTGAGTCTGAGGTTTCAAAAAGCGATGATGATTCGGAGTACTATGATGTTGAAGATGAAAAAGAGACGCCGGAGCCAATTTTTGTGTTCAAATTTGAGTATCAGACTAAAGCCGTATTAGAAGCAATGGAGAAAGTGAATGCTTATGGTCATTCTTCTGTTGGAACCAACGAAGAGAGTGAGAAATTTAGCGGCAGAAGGTACGAGTTTCAAGCCAAAAAGAATGTTAGTTTCTATGAGGAAGAAGCGAAAGTTGCAAGCTTTGTTGTCAGAGAAGCACCTGCTGATGACTCAAGTTGCGGTTCCATTGTGATTGAAAACGAGGGTGTTAAGCAATTGGTTACAGAAGAATGTGTTGAAGAAGAAGAAGAAGAAGCAAAAGTTGCGAGCTTTGTTGTCGAAGAAGAATCCGCTGATAAATCAAATTTTGGTTCTATTGTGACCGGAAGTGAGAGTGTTAAGCAACTGGTCACAGAAGATTGTGTTCATGAAGACATTCAAAATCCTCAGGATTCGAAGAAGAAACAAGTCGGGGAAGATTCTCATTCGGATAAAGAGGAACAATTGAAGTCTCAAGAACATGACTTTGTGGATGAGAAAGTTGCTTCTCATGAAGATAAGTTTCAGCAAGAAAAGGACTTTGTTTTAGTAGAATCTGCTTCTGATTCTGATTCGGGTTCTCTTTGTTCAAGCCCTGAGCTTTCATTTATAGGTCAATTTAGTGGTGATGGATTTTTGTCAGAAAGAGATTTTGGGGAAAGGGATGAATTTGGTGCTTTGGAAGACATCGATCTGCAGAATTTGGATGTTGGGTATGAGCCTGATGGATTTGATGAAGAAGATGAAGATATAATGGAACAACTTAAAGAGCTAGAAGAGTCAACTAGGAATCATGAACATCACAACCCAAAAGAGTCTAAACCTGAAGGATTTACTGCCAAAGATGAAAAACCAAATGAGGGTTTAGAAAATTCTGAACAGGTCAGTGGTCAAGATATGGCAGCTAAAGATTCTGAGGATCCGAACACATTGGAGACACTGTGGGAACATCAAGAATTGATAGAACAGTTGAAAATGGAGCTCAAGAAAGTCCGGGTTTCTGGTCTGCCTACTATCCTTGAAGAATCCGAGTCTCCGAATATGGAGGATTTGAAGCCATGGAAGATTGATGAAAAATACCATCATGTGGACAGAATGAGTGTGCTTCACAAGTTCTACAAGACCTACAGAGAACGAATGCGAAAATTCGACATTTTGAGTTACCAGAAGTTATATGCAATAGGATTTTTGCAGTCCAAGGACACGTTCGAAACCTTTTCAAGCTGCAAATCCTCAACTCCAGCAATCAAATCCTTTCTTTTGCAGAACTTTGGGCTATGCAAACCGAAGAAAAATTCTAATTCTGATTCTGACCCAATGGTTAAGTTCATTAGAGAATTGCATAGTGAATTGGAAGTGGTTTATGTTGGACAGTTGTGCCTTTCTTGGGAATTCCTTCAATGGCAATATGAGAAGGCATTGGAGCTTTGGGAATATGACGAATATGGATTACGTTCGTATAATGAAGTTGCCGAGGAATTTCAACAATTTCAAGTGCTCATGCAAAGATTTGTGGAGAATGAAAGCTTTCAAGGGCCAAGGGTCGAAACCTATGTCAAAAATCGATGTGTTATGCGGAATCTTATGCAAATTCCAGTGATTAGGGAGGACAGTATGAAGGAAAAAAAGAAAGCAAAGAGAAAAGGCAAAGACAATGATGATGCAGTTGGAAGTGATATTCTAGTAGAGATATTGCAGGAATCAATAAGGACCATTTGGCGATTCATACGAGCTGATAAATCTGCTAATACAACGGATGCTTGTCGAAAAAGATTAAAGTTGGAAGTTGAAGACTCCGTGGATCCAAAGCTATTAGTGGAAGTTCAAGCAGATCTTCAAAAGAAAGAAAAGAAGCTTAAAGAGCTGTCCCGGAGTGAGAACTGCATACTAAAGAGATTCAAAAAGCACCATGAAGAAGAAGAGGAAGAAGAGAGCGCAGAACACCTCTACTTCTTTTGTCAAGTTGACATCAAATTAGTGTCGAGGGTTTTAAACATGTCAAGCATAACAACTGATCAACTAGTCTGGTGTCACAATAAGCTAAGCAGGATACATATTGTGAACCGAAAGATTCGTGTAGATCCTGCTTTCTTACTATTTCCATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

739

Amino Acids

85.73

Weight (kDa)

4.75

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 486 - 738 9.1e-94 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 301
Acc36I ACCTGC 1 cut(s) 301
AccB7I CCANNNNNTGG 1 cut(s) 1198
AciI CCGC 4 cut(s) 204, 312, 423, 1687
AclWI GGATC 5 cut(s) 1025, 1038, 1907, 1920, 2186
AcuI CTGAAG 1 cut(s) 957
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 2 cut(s) 53, 215
AfiI CCNNNNNNNGG 3 cut(s) 971, 1198, 1498
AflIII ACRYGT 2 cut(s) 1314, 2106
AhlI ACTAGT 1 cut(s) 2131
AjuI GAANNNNNNNTTGG 4 cut(s) 747, 779, 1301, 1333
Alw21I GWGCWC 2 cut(s) 1095, 1611
Alw26I GTCTC 4 cut(s) 71, 588, 1040, 1149
AlwI GGATC 5 cut(s) 1025, 1038, 1907, 1920, 2186
AoxI GGCC 1 cut(s) 1646
ApeKI GCWGC 2 cut(s) 1013, 1335
ArsI GACNNNNNNTTYG 2 cut(s) 1650, 1682
Asp700I GAANNNNTTC 3 cut(s) 507, 1506, 1638
AspLEI GCGC 1 cut(s) 2048
AspS9I GGNCC 2 cut(s) 1646, 1821
AsuC2I CCSGG 2 cut(s) 1106, 1981
AsuHPI GGTGA 1 cut(s) 749
AsuII TTCGAA 2 cut(s) 527, 1320
AvaII GGWCC 1 cut(s) 1821
AxyI CCTNAGG 1 cut(s) 519
BamHI GGATCC 2 cut(s) 1030, 1912
BanII GRGCYC 1 cut(s) 1095
BbsI GAAGAC 3 cut(s) 510, 801, 1908
Bbv12I GWGCWC 2 cut(s) 1095, 1611
BbvI GCAGC 2 cut(s) 1025, 1322
BccI CCATC 3 cut(s) 734, 830, 1200
BceAI ACGGC 1 cut(s) 110
BciVI GTATCC 1 cut(s) 2154
BclI TGATCA 1 cut(s) 2125
BcnI CCSGG 2 cut(s) 1106, 1981
BcoDI GTCTC 4 cut(s) 71, 588, 1040, 1149
BcuI ACTAGT 1 cut(s) 2131
BfaI CTAG 4 cut(s) 884, 897, 1793, 2132
BfmI CTRYAG 2 cut(s) 806, 1237
BfuAI ACCTGC 1 cut(s) 301
BfuI GTATCC 1 cut(s) 2154
BglII AGATCT 1 cut(s) 1942
BisI GCNGC 3 cut(s) 205, 1014, 1336
BlpI GCTNAGC 1 cut(s) 2153
BlsI GCNGC 3 cut(s) 206, 1015, 1337
BmcAI AGTACT 1 cut(s) 53
Bme1390I CCNGG 2 cut(s) 1106, 1981
Bme18I GGWCC 1 cut(s) 1821
BmgT120I GGNCC 2 cut(s) 1646, 1821
BmiI GGNNCC 5 cut(s) 87, 178, 316, 1032, 1914
BmrFI CCNGG 2 cut(s) 1106, 1981
BmsI GCATC 2 cut(s) 1762, 1858
BpiI GAAGAC 3 cut(s) 510, 801, 1908
BpmI CTGGAG 1 cut(s) 1335
Bpu10I CCTNAGC 1 cut(s) 708
Bpu1102I GCTNAGC 1 cut(s) 2153
Bpu14I TTCGAA 2 cut(s) 527, 1320
BpuEI CTTGAG 3 cut(s) 289, 570, 1079
BpuMI CCSGG 2 cut(s) 1106, 1981
Bsa29I ATCGAT 2 cut(s) 801, 1675
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 5 cut(s) 1169, 1308, 1584, 1649, 1908
BsaWI WCCGGW 1 cut(s) 455
BsaXI ACNNNNNCTCC 2 cut(s) 1037, 1067
Bsc4I CCNNNNNNNGG 3 cut(s) 971, 1198, 1498
Bse1I ACTGG 2 cut(s) 483, 1706
Bse21I CCTNAGG 1 cut(s) 519
Bse3DI GCAATG 1 cut(s) 144
BseCI ATCGAT 2 cut(s) 801, 1675
BseDI CCNNGG 5 cut(s) 1169, 1308, 1584, 1649, 1908
BseGI GGATG 4 cut(s) 610, 781, 823, 1873
BseLI CCNNNNNNNGG 3 cut(s) 971, 1198, 1498
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 4 cut(s) 12, 533, 699, 1017
BseNI ACTGG 2 cut(s) 483, 1706
BseXI GCAGC 2 cut(s) 1025, 1322
BshFI GGCC 1 cut(s) 1648
BshVI ATCGAT 2 cut(s) 801, 1675
BsiHKAI GWGCWC 2 cut(s) 1095, 1611
BsiSI CCGG 4 cut(s) 83, 456, 1105, 1981
BslFI GGGAC 1 cut(s) 1963
BslI CCNNNNNNNGG 3 cut(s) 971, 1198, 1498
BsmAI GTCTC 4 cut(s) 71, 588, 1040, 1149
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 1963
BsmI GAATGC 2 cut(s) 157, 1254
BsnI GGCC 1 cut(s) 1648
Bsp119I TTCGAA 2 cut(s) 527, 1320
Bsp1286I GDGCHC 2 cut(s) 1095, 1611
Bsp143I GATC 6 cut(s) 802, 1030, 1912, 1942, 2125, 2191
Bsp1720I GCTNAGC 1 cut(s) 2153
Bsp19I CCATGG 1 cut(s) 1169
BspACI CCGC 4 cut(s) 204, 312, 423, 1687
BspANI GGCC 1 cut(s) 1648
BspCNI CTCAG 4 cut(s) 13, 532, 700, 1018
BspDI ATCGAT 2 cut(s) 801, 1675
BspHI TCATGA 3 cut(s) 499, 622, 904
BspLI GGNNCC 5 cut(s) 87, 178, 316, 1032, 1914
BspMAI CTGCAG 1 cut(s) 810
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 5 cut(s) 1025, 1038, 1907, 1920, 2186
BspT104I TTCGAA 2 cut(s) 527, 1320
BsrDI GCAATG 1 cut(s) 144
BsrI ACTGG 2 cut(s) 483, 1706
BssECI CCNNGG 5 cut(s) 1169, 1308, 1584, 1649, 1908
BssMI GATC 6 cut(s) 802, 1030, 1912, 1942, 2125, 2191
BssNI GRCGYC 1 cut(s) 80
BssT1I CCWWGG 3 cut(s) 1169, 1308, 1649
Bst4CI ACNGT 4 cut(s) 1053, 1080, 1485, 1724
Bst6I CTCTTC 4 cut(s) 180, 882, 2025, 2034
BstACI GRCGYC 1 cut(s) 80
BstAPI GCANNNNNTGC 1 cut(s) 395
BstBI TTCGAA 2 cut(s) 527, 1320
BstC8I GCNNGC 2 cut(s) 271, 400
BstDEI CTNAG 5 cut(s) 21, 519, 708, 1026, 2153
BstDSI CCRYGG 2 cut(s) 1169, 1908
BstF5I GGATG 4 cut(s) 610, 781, 823, 1873
BstHHI GCGC 1 cut(s) 2048
BstKTI GATC 6 cut(s) 805, 1033, 1915, 1945, 2128, 2194
BstMAI GTCTC 4 cut(s) 71, 588, 1040, 1149
BstMBI GATC 6 cut(s) 802, 1030, 1912, 1942, 2125, 2191
BstMWI GCNNNNNNNGC 3 cut(s) 266, 395, 1972
BstNSI RCATGY 1 cut(s) 2110
BstSCI CCNGG 2 cut(s) 1104, 1979
BstSFI CTRYAG 2 cut(s) 806, 1237
BstV1I GCAGC 2 cut(s) 1025, 1322
BstV2I GAAGAC 3 cut(s) 510, 801, 1908
BstX2I RGATCY 4 cut(s) 1030, 1912, 1942, 2191
BstYI RGATCY 4 cut(s) 1030, 1912, 1942, 2191
Bsu15I ATCGAT 2 cut(s) 801, 1675
Bsu36I CCTNAGG 1 cut(s) 519
BsuI GTATCC 1 cut(s) 2154
BsuRI GGCC 1 cut(s) 1648
BsuTUI ATCGAT 2 cut(s) 801, 1675
BtgI CCRYGG 2 cut(s) 1169, 1908
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 4 cut(s) 610, 781, 823, 1873
BtsIMutI CAGTG 3 cut(s) 1003, 1049, 1713
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 2 cut(s) 271, 400
CciI TCATGA 3 cut(s) 499, 622, 904
CfoI GCGC 1 cut(s) 2048
Cfr13I GGNCC 2 cut(s) 1646, 1821
ClaI ATCGAT 2 cut(s) 801, 1675
CseI GACGC 1 cut(s) 88
Csp6I GTAC 2 cut(s) 52, 214
CviQI GTAC 2 cut(s) 52, 214
DdeI CTNAG 5 cut(s) 21, 519, 708, 1026, 2153
DpnI GATC 6 cut(s) 804, 1032, 1914, 1944, 2127, 2193
DpnII GATC 6 cut(s) 802, 1030, 1912, 1942, 2125, 2191
DraI TTTAAA 1 cut(s) 2103
Eam1104I CTCTTC 4 cut(s) 180, 882, 2025, 2034
EarI CTCTTC 4 cut(s) 180, 882, 2025, 2034
Ecl136II GAGCTC 1 cut(s) 1093
Eco130I CCWWGG 3 cut(s) 1169, 1308, 1649
Eco24I GRGCYC 1 cut(s) 1095
Eco47I GGWCC 1 cut(s) 1821
Eco53kI GAGCTC 1 cut(s) 1093
Eco57I CTGAAG 1 cut(s) 957
Eco81I CCTNAGG 1 cut(s) 519
EcoICRI GAGCTC 1 cut(s) 1093
EcoRI GAATTC 1 cut(s) 1502
EcoT14I CCWWGG 3 cut(s) 1169, 1308, 1649
EcoT38I GRGCYC 1 cut(s) 1095
ErhI CCWWGG 3 cut(s) 1169, 1308, 1649
Esp3I CGTCTC 1 cut(s) 71
FalI AAGNNNNNCTT 2 cut(s) 1929, 1961
FaqI GGGAC 1 cut(s) 1963
FbaI TGATCA 1 cut(s) 2125
Fnu4HI GCNGC 3 cut(s) 205, 1014, 1336
FokI GGATG 4 cut(s) 617, 788, 830, 1880
FriOI GRGCYC 1 cut(s) 1095
Fsp4HI GCNGC 3 cut(s) 205, 1014, 1336
FspBI CTAG 4 cut(s) 884, 897, 1793, 2132
GlaI GCGC 1 cut(s) 2047
GluI GCNGC 3 cut(s) 205, 1014, 1336
GsuI CTGGAG 1 cut(s) 1335
HaeIII GGCC 1 cut(s) 1648
HapII CCGG 4 cut(s) 83, 456, 1105, 1981
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 2048
Hin1I GRCGYC 1 cut(s) 80
Hin6I GCGC 1 cut(s) 2046
HinP1I GCGC 1 cut(s) 2046
HincII GTYRAC 2 cut(s) 894, 2077
HindII GTYRAC 2 cut(s) 894, 2077
HindIII AAGCTT 3 cut(s) 271, 1636, 1964
HpaII CCGG 4 cut(s) 83, 456, 1105, 1981
HphI GGTGA 1 cut(s) 749
Hpy166II GTNNAC 4 cut(s) 894, 1201, 2077, 2173
Hpy188III TCNNGA 8 cut(s) 500, 521, 587, 623, 905, 1004, 1064, 1096
Hpy8I GTNNAC 4 cut(s) 894, 1201, 2077, 2173
HpyAV CCTTC 5 cut(s) 204, 932, 1517, 1519, 1723
HpyCH4III ACNGT 4 cut(s) 1053, 1080, 1485, 1724
HpyCH4IV ACGT 2 cut(s) 1316, 1564
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 395, 1972
HpyF3I CTNAG 5 cut(s) 21, 519, 708, 1026, 2153
HpySE526I ACGT 2 cut(s) 1316, 1564
Hsp92I GRCGYC 1 cut(s) 80
HspAI GCGC 1 cut(s) 2046
Ksp22I TGATCA 1 cut(s) 2125
Kzo9I GATC 6 cut(s) 802, 1030, 1912, 1942, 2125, 2191
LmnI GCTCC 3 cut(s) 85, 1090, 1534
Lsp1109I GCAGC 2 cut(s) 1025, 1322
LweI GCATC 2 cut(s) 1762, 1858
MaeI CTAG 4 cut(s) 884, 897, 1793, 2132
MaeII ACGT 2 cut(s) 1316, 1564
MaeIII GTNAC 5 cut(s) 352, 451, 481, 1271, 2141
MalI GATC 6 cut(s) 804, 1032, 1914, 1944, 2127, 2193
MboI GATC 6 cut(s) 802, 1030, 1912, 1942, 2125, 2191
MfeI CAATTG 2 cut(s) 347, 575
MflI RGATCY 4 cut(s) 1030, 1912, 1942, 2191
MhlI GDGCHC 2 cut(s) 1095, 1611
MlyI GAGTC 6 cut(s) 26, 296, 899, 935, 1151, 1898
MmeI TCCRAC 4 cut(s) 154, 1459, 1759, 1872
MroXI GAANNNNTTC 3 cut(s) 507, 1506, 1638
MseI TTAA 7 cut(s) 342, 471, 876, 1434, 1886, 1968, 2102
MspA1I CMGCKG 1 cut(s) 425
MspI CCGG 4 cut(s) 83, 456, 1105, 1981
MspR9I CCNGG 2 cut(s) 1106, 1981
MunI CAATTG 2 cut(s) 347, 575
Mva1269I GAATGC 2 cut(s) 157, 1254
MwoI GCNNNNNNNGC 3 cut(s) 266, 395, 1972
NciI CCSGG 2 cut(s) 1106, 1981
NcoI CCATGG 1 cut(s) 1169
NdeII GATC 6 cut(s) 802, 1030, 1912, 1942, 2125, 2191
NlaIV GGNNCC 5 cut(s) 87, 178, 316, 1032, 1914
NmeAIII GCCGAG 1 cut(s) 1609
NmuCI GTSAC 3 cut(s) 451, 481, 2141
NspI RCATGY 1 cut(s) 2110
NspV TTCGAA 2 cut(s) 527, 1320
PagI TCATGA 3 cut(s) 499, 622, 904
PaqCI CACCTGC 1 cut(s) 301
PciI ACATGT 1 cut(s) 2106
PctI GAATGC 2 cut(s) 157, 1254
PdmI GAANNNNTTC 3 cut(s) 507, 1506, 1638
PflMI CCANNNNNTGG 1 cut(s) 1198
PfoI TCCNGGA 1 cut(s) 1979
PkrI GCNGC 3 cut(s) 206, 1015, 1337
PleI GAGTC 6 cut(s) 25, 296, 898, 934, 1150, 1898
PpsI GAGTC 6 cut(s) 25, 296, 898, 934, 1150, 1898
PscI ACATGT 1 cut(s) 2106
Psp124BI GAGCTC 1 cut(s) 1095
PspN4I GGNNCC 5 cut(s) 87, 178, 316, 1032, 1914
PspPI GGNCC 2 cut(s) 1646, 1821
PstI CTGCAG 1 cut(s) 810
PsuI RGATCY 4 cut(s) 1030, 1912, 1942, 2191
RsaI GTAC 2 cut(s) 53, 215
RsaNI GTAC 2 cut(s) 52, 214
SacI GAGCTC 1 cut(s) 1095
SaqAI TTAA 7 cut(s) 342, 471, 876, 1434, 1886, 1968, 2102
SatI GCNGC 3 cut(s) 205, 1014, 1336
Sau3AI GATC 6 cut(s) 802, 1030, 1912, 1942, 2125, 2191
Sau96I GGNCC 2 cut(s) 1646, 1821
ScaI AGTACT 1 cut(s) 53
SchI GAGTC 6 cut(s) 26, 296, 899, 935, 1151, 1898
ScrFI CCNGG 2 cut(s) 1106, 1981
SduI GDGCHC 2 cut(s) 1095, 1611
SfaNI GCATC 2 cut(s) 1762, 1858
SfcI CTRYAG 2 cut(s) 806, 1237
SfuI TTCGAA 2 cut(s) 527, 1320
SinI GGWCC 1 cut(s) 1821
SmlI CTYRAG 3 cut(s) 304, 585, 1094
SmoI CTYRAG 3 cut(s) 304, 585, 1094
SpeI ACTAGT 1 cut(s) 2131
SsiI CCGC 4 cut(s) 204, 312, 423, 1687
SspMI CTAG 4 cut(s) 884, 897, 1793, 2132
SstI GAGCTC 1 cut(s) 1095
StyD4I CCNGG 2 cut(s) 1104, 1979
StyI CCWWGG 3 cut(s) 1169, 1308, 1649
TaaI ACNGT 4 cut(s) 1053, 1080, 1485, 1724
TaiI ACGT 2 cut(s) 1319, 1567
TaqI TCGA 9 cut(s) 411, 527, 801, 1260, 1320, 1656, 1675, 1876, 2093
TatI WGTACW 1 cut(s) 51
TauI GCSGC 1 cut(s) 207
Tru1I TTAA 7 cut(s) 342, 471, 876, 1434, 1886, 1968, 2102
Tru9I TTAA 7 cut(s) 342, 471, 876, 1434, 1886, 1968, 2102
TscAI CASTG 3 cut(s) 1003, 1056, 1713
TseFI GTSAC 3 cut(s) 451, 481, 2141
TseI GCWGC 2 cut(s) 1013, 1335
Tsp45I GTSAC 3 cut(s) 451, 481, 2141
TspGWI ACGGA 2 cut(s) 1880, 1897
TspRI CASTG 3 cut(s) 1003, 1056, 1713
Van91I CCANNNNNTGG 1 cut(s) 1198
VpaK11BI GGWCC 1 cut(s) 1821
XceI RCATGY 1 cut(s) 2110
XmnI GAANNNNTTC 3 cut(s) 507, 1506, 1638
XspI CTAG 4 cut(s) 884, 897, 1793, 2132
ZrmI AGTACT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.