FvH4_6g01220

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002986
Physical Location & Seq
Reverse (-)
665032 .. 666837
1806 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01220.t1

Sequence Viewer

Length: 1806 bp
ATGCTTGCTCAGTTTTCCGACTCCATTGTGTTCAGTTTTTTCAGCACACTCTTGATCGTAGAAGAGATTTCCCCGATGAGGAACTCCGGTTTCTCTACCACTGTTCCATGTCGGAACAGTTACGAAGAAGAAGAACATGATGATTCTGAATTCTTTGATGCTAATGAAGAAGAGTGCCCAGAGCCAACTTTTGTGTTTAAATTTGAGTACCAGACTAGGGAGATATTAAAGACACGGGAAAAAGTGGATGAGATTGGAGATTATGTAGCTTTTCAAGCCGGGAAGAAGAGTACTAGTTGCTTCATTGAAGAATCACAAAATGGATCTGCTCAGAATTCACACTGTTATTCTATAGAAGCAAATGATCATGTGGAAGAAGGTGATCAGGGTTTTGGAGATGGTGCATTTGAGGAAGAGGAGGTTGAGAAGAGAATAGTGATTGAGGAAAACTCTGTTTCGGATGACGAAGAAGAAGCAGATGTTAATAATGAAGTGAAGTTTGGTTCTGTTGTGAAGGAAGACATGTCACATTCAGATGAGGAAAGTGACAGTACTGGTGATGAGACGGTGGCTACTACTAGTCATGAAGATGAGTTGGTTTCCAGTTCGGAATTCGATTCTGATTTGATATGTTCAAGGGAGGATGATGATGATGAACTAAGTGCTTTGGAAGACATTGGATTGCGCAAGCCTGATGGTTTTGATGACAAAGATGTTTCTGAGGACACATTGGAGGCTTTGTGTGAACATTTAAAGTCGATAGATAAGTTGAAAATGGAGCTCAGGAAAGTTAGAGCTACTACTGATCTGCCTACTATTTTTGAAGAATCAGAGTCTCTGGATATGGATGACTTGAAGCCACTCAAGATTTTTGATATATACCAACATGGAGATACATATAAGAGTTACAAGTTTCAAATGCGCAAGTTGGATATTTTGAATTTTGCAATAGGTGATCTGGAGTCCAAACATTCACTTAAATTGTTTTCGAGCTACAAATCATTTGCTCCAGCTATCAAATCCTTTCTTTCCAACTTGAGGCTTTGTAGACTCAAAATAGATTGTGATCCAATGCATAGTGAGTTAGAAAAGATCTATGTTGGGCAGTTGTGCCTTTCTTGGGAGTTTCTTCAGTGGCAGTATGAGAAGGCCTTAGAATATGCCCCCAATCGAATACATTCATACAATGAAGTCGCAGAAGAGTTGCAGCAGTTTCAAGTGCTCCTACAAAGATTTGTAGAGGATGAACATTTTCAAGGGCAAAGGGTCGAAAACTATGTTAAAAGTCGATATGCTATGCATAAGCTTCTACAAGTTCCAGAAATCAGAGAGGACAGATTGGAGGAAGGAAAGGAAGGTAATGAAGTTACCACTAACATTGTCGAGACATTGAGAGAATCAATAAGGACCATATGGCGATTCATTCGAGCTGATAAAAATGCCAACATAACTACTGCTTGTCAAAAAAGATCACTTCCATCTGGCTTGGATCCAGAGCTTTTAAAACAAATTCAAGCAGATCTTCGGACAAAAGAAAGGATGCTTAAGGAGCTAAATAGGAGTCAGAACCGGATCATAAAGAGATTCCGAAAGCATGAAGAAGAAGGAGAAGAAGATGAAGAGAGCGCAGATCATGGTCACCTTAGCTTCTTTTCTCGAGTTGACATCAGATTGGTTTCAAGGGTTCTGAACATGTCAACTATAACACAAGATCAGCTAGTGTGGTGTCAAAGTAAGTTGAGCAAGATACACTTCGTGAGGCGCAAGGTTCATGAAGATCCCTCTTTCTTGTGGTTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

602

Amino Acids

69.87

Weight (kDa)

4.66

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 360 - 600 1.8e-78 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 686, 923
AccI GTMKAC 1 cut(s) 1048
AclWI GGATC 6 cut(s) 331, 1061, 1484, 1497, 1580, 1772
AcsI RAATTY 6 cut(s) 149, 200, 334, 611, 940, 1509
AcuI CTGAAG 1 cut(s) 1115
AdeI CACNNNGTG 1 cut(s) 1756
AfaI GTAC 3 cut(s) 209, 292, 553
AfiI CCNNNNNNNGG 4 cut(s) 78, 217, 1038, 1120
AflII CTTAAG 1 cut(s) 1544
AflIII ACRYGT 2 cut(s) 522, 1692
AhlI ACTAGT 2 cut(s) 293, 578
AjuI GAANNNNNNNTTGG 2 cut(s) 483, 515
Alw21I GWGCWC 2 cut(s) 783, 1224
Alw26I GTCTC 3 cut(s) 557, 840, 1379
AlwI GGATC 6 cut(s) 331, 1061, 1484, 1497, 1580, 1772
Ama87I CYCGRG 1 cut(s) 1656
AoxI GGCC 1 cut(s) 1149
ApeKI GCWGC 1 cut(s) 1207
ApoI RAATTY 6 cut(s) 149, 200, 334, 611, 940, 1509
Asp700I GAANNNNTTC 2 cut(s) 1177, 1251
AspLEI GCGC 4 cut(s) 687, 924, 1628, 1764
AspS9I GGNCC 1 cut(s) 1407
AsuC2I CCSGG 1 cut(s) 280
AsuHPI GGTGA 4 cut(s) 392, 569, 965, 1631
AvaI CYCGRG 1 cut(s) 1656
AvaII GGWCC 1 cut(s) 1407
BaeGI GKGCMC 1 cut(s) 179
BamHI GGATCC 1 cut(s) 1489
BanII GRGCYC 1 cut(s) 783
BbsI GAAGAC 2 cut(s) 525, 678
Bbv12I GWGCWC 2 cut(s) 783, 1224
BbvI GCAGC 1 cut(s) 1219
BccI CCATC 3 cut(s) 392, 689, 1486
BclI TGATCA 2 cut(s) 364, 382
BcnI CCSGG 1 cut(s) 280
BcoDI GTCTC 3 cut(s) 557, 840, 1379
BcuI ACTAGT 2 cut(s) 293, 578
BfaI CTAG 4 cut(s) 216, 294, 579, 1718
BfmI CTRYAG 1 cut(s) 351
BfrI CTTAAG 1 cut(s) 1544
BglII AGATCT 2 cut(s) 1092, 1519
BisI GCNGC 1 cut(s) 1208
BlsI GCNGC 1 cut(s) 1209
BmcAI AGTACT 2 cut(s) 292, 553
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 1407
BmeT110I CYCGRG 1 cut(s) 1656
BmgT120I GGNCC 1 cut(s) 1407
BmiI GGNNCC 1 cut(s) 1491
BmrFI CCNGG 1 cut(s) 280
BmsI GCATC 2 cut(s) 148, 1530
BpiI GAAGAC 2 cut(s) 525, 678
BplI GAGNNNNNCTC 2 cut(s) 434, 466
BpmI CTGGAG 2 cut(s) 980, 993
Bpu10I CCTNAGC 2 cut(s) 782, 1643
BpuEI CTTGAG 2 cut(s) 848, 1057
BpuMI CCSGG 1 cut(s) 280
BsaBI GATNNNNATC 1 cut(s) 1065
BsaWI WCCGGW 2 cut(s) 86, 1569
BsaXI ACNNNNNCTCC 4 cut(s) 249, 279, 725, 755
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 217, 1038, 1120
Bse1I ACTGG 2 cut(s) 559, 603
Bse8I GATNNNNATC 1 cut(s) 1065
BseGI GGATG 6 cut(s) 253, 466, 649, 853, 1249, 1545
BseJI GATNNNNATC 1 cut(s) 1065
BseLI CCNNNNNNNGG 4 cut(s) 78, 217, 1038, 1120
BseMII CTCAG 4 cut(s) 23, 344, 711, 796
BseNI ACTGG 2 cut(s) 559, 603
BseRI GAGGAG 1 cut(s) 431
BseSI GKGCMC 1 cut(s) 179
BseXI GCAGC 1 cut(s) 1219
BshFI GGCC 1 cut(s) 1151
BsiHKAI GWGCWC 2 cut(s) 783, 1224
BsiHKCI CYCGRG 1 cut(s) 1656
BsiSI CCGG 3 cut(s) 87, 279, 1570
BslI CCNNNNNNNGG 4 cut(s) 78, 217, 1038, 1120
BsmAI GTCTC 3 cut(s) 557, 840, 1379
BsmBI CGTCTC 1 cut(s) 557
BsnI GGCC 1 cut(s) 1151
BsoBI CYCGRG 1 cut(s) 1656
Bsp1286I GDGCHC 3 cut(s) 179, 783, 1224
BspANI GGCC 1 cut(s) 1151
BspCNI CTCAG 4 cut(s) 22, 343, 712, 795
BspHI TCATGA 2 cut(s) 583, 1771
BspLI GGNNCC 1 cut(s) 1491
BspPI GGATC 6 cut(s) 331, 1061, 1484, 1497, 1580, 1772
BspTI CTTAAG 1 cut(s) 1544
BsrI ACTGG 2 cut(s) 559, 603
Bst4CI ACNGT 5 cut(s) 103, 119, 344, 551, 568
Bst6I CTCTTC 7 cut(s) 57, 165, 281, 408, 422, 1194, 1614
BstAFI CTTAAG 1 cut(s) 1544
BstC8I GCNNGC 2 cut(s) 6, 689
BstDEI CTNAG 7 cut(s) 9, 330, 659, 720, 782, 1153, 1643
BstEII GGTNACC 1 cut(s) 1637
BstF5I GGATG 6 cut(s) 253, 466, 649, 853, 1249, 1545
BstHHI GCGC 4 cut(s) 687, 924, 1628, 1764
BstMAI GTCTC 3 cut(s) 557, 840, 1379
BstMWI GCNNNNNNNGC 2 cut(s) 275, 1549
BstNSI RCATGY 2 cut(s) 526, 1696
BstPI GGTNACC 1 cut(s) 1637
BstSCI CCNGG 1 cut(s) 278
BstSFI CTRYAG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 179
BstV1I GCAGC 1 cut(s) 1219
BstV2I GAAGAC 2 cut(s) 525, 678
BstX2I RGATCY 5 cut(s) 323, 1092, 1489, 1519, 1777
BstYI RGATCY 5 cut(s) 323, 1092, 1489, 1519, 1777
BsuRI GGCC 1 cut(s) 1151
BtsCI GGATG 6 cut(s) 253, 466, 649, 853, 1249, 1545
BtsIMutI CAGTG 3 cut(s) 99, 340, 1139
Cac8I GCNNGC 2 cut(s) 6, 689
CciI TCATGA 2 cut(s) 583, 1771
CfoI GCGC 4 cut(s) 687, 924, 1628, 1764
Cfr13I GGNCC 1 cut(s) 1407
Csp6I GTAC 3 cut(s) 208, 291, 552
CspCI CAANNNNNGTGG 2 cut(s) 1360, 1395
CviQI GTAC 3 cut(s) 208, 291, 552
DdeI CTNAG 7 cut(s) 9, 330, 659, 720, 782, 1153, 1643
DraI TTTAAA 3 cut(s) 199, 753, 1503
DraIII CACNNNGTG 1 cut(s) 1756
Eam1104I CTCTTC 7 cut(s) 57, 165, 281, 408, 422, 1194, 1614
EarI CTCTTC 7 cut(s) 57, 165, 281, 408, 422, 1194, 1614
Ecl136II GAGCTC 1 cut(s) 781
Eco147I AGGCCT 1 cut(s) 1151
Eco24I GRGCYC 1 cut(s) 783
Eco47I GGWCC 1 cut(s) 1407
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 1 cut(s) 1115
Eco88I CYCGRG 1 cut(s) 1656
Eco91I GGTNACC 1 cut(s) 1637
EcoICRI GAGCTC 1 cut(s) 781
EcoO65I GGTNACC 1 cut(s) 1637
EcoRI GAATTC 3 cut(s) 149, 334, 611
EcoT22I ATGCAT 2 cut(s) 1077, 1302
EcoT38I GRGCYC 1 cut(s) 783
Esp3I CGTCTC 1 cut(s) 557
FalI AAGNNNNNCTT 4 cut(s) 1506, 1538, 1736, 1768
FauNDI CATATG 1 cut(s) 1412
FbaI TGATCA 2 cut(s) 364, 382
FblI GTMKAC 1 cut(s) 1048
Fnu4HI GCNGC 1 cut(s) 1208
FokI GGATG 6 cut(s) 260, 473, 656, 860, 1256, 1552
FriOI GRGCYC 1 cut(s) 783
Fsp4HI GCNGC 1 cut(s) 1208
FspBI CTAG 4 cut(s) 216, 294, 579, 1718
FspI TGCGCA 2 cut(s) 686, 923
GlaI GCGC 4 cut(s) 686, 923, 1627, 1763
GluI GCNGC 1 cut(s) 1208
GsuI CTGGAG 2 cut(s) 980, 993
HaeIII GGCC 1 cut(s) 1151
HapII CCGG 3 cut(s) 87, 279, 1570
HhaI GCGC 4 cut(s) 687, 924, 1628, 1764
Hin6I GCGC 4 cut(s) 685, 922, 1626, 1762
HinP1I GCGC 4 cut(s) 685, 922, 1626, 1762
HincII GTYRAC 2 cut(s) 1663, 1698
HindII GTYRAC 2 cut(s) 1663, 1698
HindIII AAGCTT 1 cut(s) 1304
HpaII CCGG 3 cut(s) 87, 279, 1570
HphI GGTGA 4 cut(s) 392, 569, 965, 1631
Hpy166II GTNNAC 4 cut(s) 746, 1049, 1663, 1698
Hpy8I GTNNAC 4 cut(s) 746, 1049, 1663, 1698
HpyAV CCTTC 6 cut(s) 371, 508, 1141, 1340, 1349, 1598
HpyCH4III ACNGT 5 cut(s) 103, 119, 344, 551, 568
HpyCH4V TGCA 5 cut(s) 404, 947, 1075, 1207, 1300
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 1549
HpyF3I CTNAG 7 cut(s) 9, 330, 659, 720, 782, 1153, 1643
HspAI GCGC 4 cut(s) 685, 922, 1626, 1762
Ksp22I TGATCA 2 cut(s) 364, 382
LmnI GCTCC 4 cut(s) 778, 1012, 1227, 1549
Lsp1109I GCAGC 1 cut(s) 1219
LweI GCATC 2 cut(s) 148, 1530
MaeI CTAG 4 cut(s) 216, 294, 579, 1718
MaeIII GTNAC 6 cut(s) 119, 525, 545, 905, 1366, 1637
MflI RGATCY 5 cut(s) 323, 1092, 1489, 1519, 1777
MhlI GDGCHC 3 cut(s) 179, 783, 1224
MluCI AATT 7 cut(s) 149, 200, 334, 611, 940, 980, 1509
MlyI GAGTC 5 cut(s) 14, 842, 971, 1044, 1570
MmeI TCCRAC 4 cut(s) 42, 92, 909, 1056
Mph1103I ATGCAT 2 cut(s) 1077, 1302
MroXI GAANNNNTTC 2 cut(s) 1177, 1251
MseI TTAA 9 cut(s) 198, 227, 483, 752, 978, 1281, 1502, 1545, 1804
MslI CAYNNNNRTG 2 cut(s) 534, 588
MspCI CTTAAG 1 cut(s) 1544
MspI CCGG 3 cut(s) 87, 279, 1570
MspR9I CCNGG 1 cut(s) 280
MwoI GCNNNNNNNGC 2 cut(s) 275, 1549
NciI CCSGG 1 cut(s) 280
NdeI CATATG 1 cut(s) 1412
NlaIV GGNNCC 1 cut(s) 1491
NmuCI GTSAC 3 cut(s) 525, 545, 1637
NsbI TGCGCA 2 cut(s) 686, 923
NsiI ATGCAT 2 cut(s) 1077, 1302
NspI RCATGY 2 cut(s) 526, 1696
PaeR7I CTCGAG 1 cut(s) 1656
PagI TCATGA 2 cut(s) 583, 1771
PceI AGGCCT 1 cut(s) 1151
PciI ACATGT 2 cut(s) 522, 1692
PdmI GAANNNNTTC 2 cut(s) 1177, 1251
PfeI GAWTC 7 cut(s) 143, 311, 617, 827, 1397, 1419, 1584
PkrI GCNGC 1 cut(s) 1209
PleI GAGTC 5 cut(s) 14, 841, 970, 1044, 1569
PpsI GAGTC 5 cut(s) 14, 841, 970, 1044, 1569
PscI ACATGT 2 cut(s) 522, 1692
Psp124BI GAGCTC 1 cut(s) 783
PspEI GGTNACC 1 cut(s) 1637
PspN4I GGNNCC 1 cut(s) 1491
PspPI GGNCC 1 cut(s) 1407
PsuI RGATCY 5 cut(s) 323, 1092, 1489, 1519, 1777
RsaI GTAC 3 cut(s) 209, 292, 553
RsaNI GTAC 3 cut(s) 208, 291, 552
RseI CAYNNNNRTG 2 cut(s) 534, 588
SacI GAGCTC 1 cut(s) 783
SaqAI TTAA 9 cut(s) 198, 227, 483, 752, 978, 1281, 1502, 1545, 1804
SatI GCNGC 1 cut(s) 1208
Sau96I GGNCC 1 cut(s) 1407
ScaI AGTACT 2 cut(s) 292, 553
SchI GAGTC 5 cut(s) 14, 842, 971, 1044, 1570
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 3 cut(s) 179, 783, 1224
SfaNI GCATC 2 cut(s) 148, 1530
SfcI CTRYAG 1 cut(s) 351
Sfr274I CTCGAG 1 cut(s) 1656
SinI GGWCC 1 cut(s) 1407
SlaI CTCGAG 1 cut(s) 1656
SmiMI CAYNNNNRTG 2 cut(s) 534, 588
SmlI CTYRAG 4 cut(s) 863, 1036, 1544, 1656
SmoI CTYRAG 4 cut(s) 863, 1036, 1544, 1656
SpeI ACTAGT 2 cut(s) 293, 578
Sse9I AATT 7 cut(s) 149, 200, 334, 611, 940, 980, 1509
SseBI AGGCCT 1 cut(s) 1151
SspMI CTAG 4 cut(s) 216, 294, 579, 1718
SstI GAGCTC 1 cut(s) 783
StuI AGGCCT 1 cut(s) 1151
StyD4I CCNGG 1 cut(s) 278
TaaI ACNGT 5 cut(s) 103, 119, 344, 551, 568
TaqI TCGA 9 cut(s) 615, 758, 989, 1171, 1269, 1288, 1383, 1426, 1657
TasI AATT 7 cut(s) 149, 200, 334, 611, 940, 980, 1509
TatI WGTACW 2 cut(s) 290, 551
TfiI GAWTC 7 cut(s) 143, 311, 617, 827, 1397, 1419, 1584
Tru1I TTAA 9 cut(s) 198, 227, 483, 752, 978, 1281, 1502, 1545, 1804
Tru9I TTAA 9 cut(s) 198, 227, 483, 752, 978, 1281, 1502, 1545, 1804
TscAI CASTG 3 cut(s) 106, 347, 1139
TseFI GTSAC 3 cut(s) 525, 545, 1637
TseI GCWGC 1 cut(s) 1207
Tsp45I GTSAC 3 cut(s) 525, 545, 1637
TspRI CASTG 3 cut(s) 106, 347, 1139
Vha464I CTTAAG 1 cut(s) 1544
VpaK11BI GGWCC 1 cut(s) 1407
XapI RAATTY 6 cut(s) 149, 200, 334, 611, 940, 1509
XceI RCATGY 2 cut(s) 526, 1696
XhoI CTCGAG 1 cut(s) 1656
XmiI GTMKAC 1 cut(s) 1048
XmnI GAANNNNTTC 2 cut(s) 1177, 1251
XspI CTAG 4 cut(s) 216, 294, 579, 1718
ZrmI AGTACT 2 cut(s) 292, 553
Zsp2I ATGCAT 2 cut(s) 1077, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.