FvH4_6g48640

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002986
Physical Location & Seq
Forward (+)
36616639 .. 36620740
4102 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g48640.t4

Sequence Viewer

Length: 2397 bp
ATGGGCGGTTCTTCTGATTTTGTTTTCAGAAGAAATATGTCTCCTTTTTCTGGGTCTTTCTCGGTTTTTGTCTCCTCCCTTTTGCATACTCTGTTCCGATTTTTCAGTTCAATCTTTGTCAGGTTCGATAAAGAACAGAGTTCTCTGGTGAACGAGACTTGCTCGTTTATGGAGCCAGCAGCTATGCCCCAAAAAGCTGAGTCTGACGTTTCGAGAAGCGATGATGATTCAGAATACTATGATGCTGAAGAAGAAAAAGAGACTCCAGAGCCAAGTTTTGTTTTCAAATTTGAGTATCAGACTAAAGCTGTATTAGAAGCAATGGAGAAAGTGAATGCTTATGGTCATTCTTCTGTTGTCGAGAAAGAGAATGAGTTCCTGCCTGAGAAATTTAGCAGCAGAAGGTATGAGTTTCAAGCCGAGAAGCATGTTAGTTTCTATGAGGAAGAAGCGAAAGTTGCAAGCTTTGTTGTCAGAGAAGCATCTGCTGATGACTCAAATTGTGGTTCCATGGTGATTGAAAATGAGGGTGTTAAGGAATTGGTTACAGAAGAATGTGTTCAAAAAGAAGAAGCAAAAGTTGCAAGCTTTGTTCTCAAAGAAGAATCTGCTGAAAACTCAAATTTTGGTTCCATTGTGACTGAAGATGATGTTGTTGAGCAATTGGTTGCAGAAGAATGTGTTCGTAAAGAAATTCATATTCCTCAGGATTCAGAGAAGAAACAAGTTGGGGAAGATTCTCATACAGATAAAGATGAGCAATTGAAGTCTCAAGAACATGATGAGAAAAATGTTGCTTCTCATGAAGATAAGTTTTTGCAAGAAAAGGAATATGTTGTAGTAGAATCTGCTTCTGATTCTGATTCTGATTCGAGTTCGCTTTGTTCTAGCCCTGAGGTTTCATTCATGGGTCAATTTAGTGATGATGGGTTCTTGTTAGAAACAGAGTTTGGGGAAAGGGTTGAATTTGGTGCTTTGGAAGACATTGATTTGCAGAATATAGATGTTGGATATGAGCCTGATGAATTTGATGAAGAAGACGAAGATGTAATGGAACAACTTGAAGAGCTAGAACAGTCAACTAGGAATGATGAACAACATAACCCAAAAGAGTCCACACCTGAAGGCTTTACTGCCAAAGATGAAAAACCAAACGAGGGTTTAGAAAATTCTGAAAAGGTCAGTGGTCAAGATTTGGCAGCTAATGATTCTGGGGATCCAAACACATTGGAAACATTGTGGGAACATCAAGAATTGATAGAACAGTTGAAAATGGAGCTCAAGAAAGTCAGAGGTTCTGGTCTGCCTACTATCCTTGAAGAATCCGAGTCTCCAAATATGGAGGATTTGAAGCCATGGAAGATTGATGAAAAATACCACCATGGAGACAGAATGAGTGTGCTTCACAAGTTCTACAAGAGCTACAGAGAACGAATGCGAAAATTCGACATATTGAGCTATCAGAAGTTATATGCAATAGGATTTTTGCAGTCCAAGGACACATTAGAAACCTTTTCAAGCTGCAAATCCTCAACTCCAGCAATCAAATCATTTCTTTTGCAGAACTTTGGGCTATGCAAACCGAAGAAAAATTCTGATTCTGACCCAATGGTTAAGTTCATTAGAGAATTGCACAGTGAATTGGAAGTGGTTTATATTGGACAGTTGTGCCTTTCTTGGGAATTCCTTCAATGGCAATACGAGAAGGCATTGGAGATTTGGGAATACGATGAATATGGATTACGTTCGTATAATGAAGTTGCCGAGGAATTCCAACAGTTTCAAGTGCTCATGCAACGATTTGTGGAGAATGAACGCTTTCAAGGGCCAAGGGTAGAACATTATGTCAAAAATCGATGTGTTATGCGGAATCTTATGCAAATTCCAGTGATTAGGGAGGACAGTATGAAGATAAAAAAGAAAGCAAAGAGAGGGAAAGACATTGATGATGCAATTGGAAGTGATCTTCTAGTAGAGATATTGCAGGAATCAATAAGGACCATGTGGCGATTCATACGAGCTGATAAATCTGCTAATACAGCGGATGCTTGTCGAAAAAGATCGAAGTTGGAACTTGAAGACTCCTTGGATCCAAAGCTATTAGCCGAAGTTCAAGCAGATCTTCAAAAGAAAGAGAGGAAGCTCAAAGAACTGTTGAGGAGTGAGAACTGCATACTAAAGAGATTCAAAAAGCAACACGAAGAAGAAGAGGAAGAAGAGAGCCCAGAACACCTCTACTTCTTTTGTCAAGTTGACATCAGATTAGTGTCGAGGGTTTTAAACATGTCAAGCATAACATCTGATCAATTAGTCTGGTGCCACAATAAGCTAAGCAGGATACATGTTGTGAACCGGAAAATCCGTGTAGATCCTTCTTTCTTACTATTTCCATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

799

Amino Acids

92.61

Weight (kDa)

4.73

Isoelectric Point (pI)

55.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 490 - 741 7.9e-93 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2316
AciI CCGC 3 cut(s) 6, 1867, 2042
AclWI GGATC 5 cut(s) 1211, 1224, 2084, 2097, 2363
AcuI CTGAAG 3 cut(s) 267, 663, 1143
AfiI CCNNNNNNNGG 3 cut(s) 50, 1157, 1678
AflIII ACRYGT 2 cut(s) 2283, 2341
AjuI GAANNNNNNNTTGG 2 cut(s) 933, 965
Alw21I GWGCWC 2 cut(s) 1281, 1791
Alw26I GTCTC 7 cut(s) 45, 76, 149, 254, 774, 1335, 1380
AlwI GGATC 5 cut(s) 1211, 1224, 2084, 2097, 2363
AoxI GGCC 1 cut(s) 1826
ApeKI GCWGC 4 cut(s) 179, 396, 1199, 1521
Asp700I GAANNNNTTC 5 cut(s) 374, 558, 681, 1686, 1818
AspS9I GGNCC 2 cut(s) 1826, 1998
AsuHPI GGTGA 2 cut(s) 160, 526
AvaII GGWCC 1 cut(s) 1998
AxyI CCTNAGG 2 cut(s) 705, 894
BamHI GGATCC 2 cut(s) 1216, 2089
BanI GGYRCC 1 cut(s) 2316
BanII GRGCYC 2 cut(s) 1281, 2225
BbsI GAAGAC 3 cut(s) 987, 1044, 2085
Bbv12I GWGCWC 2 cut(s) 1281, 1791
BbvI GCAGC 4 cut(s) 191, 408, 1211, 1508
BccI CCATC 1 cut(s) 920
BciVI GTATCC 1 cut(s) 2331
BclI TGATCA 1 cut(s) 2302
BcoDI GTCTC 7 cut(s) 45, 76, 149, 254, 774, 1335, 1380
BfaI CTAG 4 cut(s) 888, 1070, 1083, 1970
BfmI CTRYAG 1 cut(s) 1423
BfuI GTATCC 1 cut(s) 2331
BglII AGATCT 1 cut(s) 2119
BisI GCNGC 4 cut(s) 180, 397, 1200, 1522
BlpI GCTNAGC 1 cut(s) 2330
BlsI GCNGC 4 cut(s) 181, 398, 1201, 1523
Bme18I GGWCC 1 cut(s) 1998
BmgT120I GGNCC 2 cut(s) 1826, 1998
BmiI GGNNCC 6 cut(s) 174, 508, 631, 1218, 2091, 2318
BmsI GCATC 4 cut(s) 232, 491, 1939, 2035
BpiI GAAGAC 3 cut(s) 987, 1044, 2085
BplI GAGNNNNNCTC 2 cut(s) 146, 178
BpmI CTGGAG 2 cut(s) 249, 1521
Bpu1102I GCTNAGC 1 cut(s) 2330
BpuEI CTTGAG 2 cut(s) 756, 1265
Bsa29I ATCGAT 1 cut(s) 1855
BsaJI CCNNGG 7 cut(s) 510, 1355, 1381, 1494, 1764, 1829, 2085
BsaWI WCCGGW 1 cut(s) 2352
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 1157, 1678
Bse1I ACTGG 1 cut(s) 1886
Bse21I CCTNAGG 2 cut(s) 705, 894
Bse3DI GCAATG 1 cut(s) 327
BseCI ATCGAT 1 cut(s) 1855
BseDI CCNNGG 7 cut(s) 510, 1355, 1381, 1494, 1764, 1829, 2085
BseGI GGATG 1 cut(s) 2050
BseLI CCNNNNNNNGG 3 cut(s) 50, 1157, 1678
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 4 cut(s) 189, 375, 719, 885
BseNI ACTGG 1 cut(s) 1886
BseRI GAGGAG 2 cut(s) 64, 2173
BseXI GCAGC 4 cut(s) 191, 408, 1211, 1508
BshFI GGCC 1 cut(s) 1828
BshNI GGYRCC 1 cut(s) 2316
BshVI ATCGAT 1 cut(s) 1855
BsiHKAI GWGCWC 2 cut(s) 1281, 1791
BsiSI CCGG 1 cut(s) 2353
BslI CCNNNNNNNGG 3 cut(s) 50, 1157, 1678
BsmAI GTCTC 7 cut(s) 45, 76, 149, 254, 774, 1335, 1380
BsmI GAATGC 2 cut(s) 340, 1440
BsnI GGCC 1 cut(s) 1828
Bsp1286I GDGCHC 3 cut(s) 1281, 1791, 2225
Bsp143I GATC 7 cut(s) 1216, 1963, 2060, 2089, 2119, 2302, 2368
Bsp1720I GCTNAGC 1 cut(s) 2330
Bsp19I CCATGG 3 cut(s) 510, 1355, 1381
BspACI CCGC 3 cut(s) 6, 1867, 2042
BspANI GGCC 1 cut(s) 1828
BspCNI CTCAG 4 cut(s) 190, 376, 718, 886
BspDI ATCGAT 1 cut(s) 1855
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 6 cut(s) 174, 508, 631, 1218, 2091, 2318
BspPI GGATC 5 cut(s) 1211, 1224, 2084, 2097, 2363
BspQI GCTCTTC 1 cut(s) 1059
BspT107I GGYRCC 1 cut(s) 2316
BsrDI GCAATG 1 cut(s) 327
BsrI ACTGG 1 cut(s) 1886
BssECI CCNNGG 7 cut(s) 510, 1355, 1381, 1494, 1764, 1829, 2085
BssMI GATC 7 cut(s) 1216, 1963, 2060, 2089, 2119, 2302, 2368
BssT1I CCWWGG 6 cut(s) 510, 1355, 1381, 1494, 1829, 2085
Bst4CI ACNGT 7 cut(s) 1077, 1266, 1637, 1665, 1779, 1904, 2154
Bst6I CTCTTC 3 cut(s) 1059, 2202, 2211
BstAPI GCANNNNNTGC 1 cut(s) 581
BstC8I GCNNGC 3 cut(s) 177, 463, 586
BstDEI CTNAG 5 cut(s) 198, 384, 705, 894, 2330
BstDSI CCRYGG 3 cut(s) 510, 1355, 1381
BstF5I GGATG 1 cut(s) 2050
BstKTI GATC 7 cut(s) 1219, 1966, 2063, 2092, 2122, 2305, 2371
BstMAI GTCTC 7 cut(s) 45, 76, 149, 254, 774, 1335, 1380
BstMBI GATC 7 cut(s) 1216, 1963, 2060, 2089, 2119, 2302, 2368
BstMWI GCNNNNNNNGC 3 cut(s) 458, 581, 2039
BstNSI RCATGY 3 cut(s) 431, 2287, 2345
BstSFI CTRYAG 1 cut(s) 1423
BstV1I GCAGC 4 cut(s) 191, 408, 1211, 1508
BstV2I GAAGAC 3 cut(s) 987, 1044, 2085
BstX2I RGATCY 4 cut(s) 1216, 2089, 2119, 2368
BstYI RGATCY 4 cut(s) 1216, 2089, 2119, 2368
Bsu15I ATCGAT 1 cut(s) 1855
Bsu36I CCTNAGG 2 cut(s) 705, 894
BsuI GTATCC 1 cut(s) 2331
BsuRI GGCC 1 cut(s) 1828
BsuTUI ATCGAT 1 cut(s) 1855
BtgI CCRYGG 3 cut(s) 510, 1355, 1381
BtgZI GCGATG 1 cut(s) 234
BtsCI GGATG 1 cut(s) 2050
BtsIMutI CAGTG 3 cut(s) 1189, 1642, 1893
Cac8I GCNNGC 3 cut(s) 177, 463, 586
CciI TCATGA 1 cut(s) 802
Cfr13I GGNCC 2 cut(s) 1826, 1998
ClaI ATCGAT 1 cut(s) 1855
DdeI CTNAG 5 cut(s) 198, 384, 705, 894, 2330
DpnI GATC 7 cut(s) 1218, 1965, 2062, 2091, 2121, 2304, 2370
DpnII GATC 7 cut(s) 1216, 1963, 2060, 2089, 2119, 2302, 2368
DraI TTTAAA 1 cut(s) 2280
Eam1104I CTCTTC 3 cut(s) 1059, 2202, 2211
EarI CTCTTC 3 cut(s) 1059, 2202, 2211
Ecl136II GAGCTC 1 cut(s) 1279
Eco130I CCWWGG 6 cut(s) 510, 1355, 1381, 1494, 1829, 2085
Eco24I GRGCYC 2 cut(s) 1281, 2225
Eco47I GGWCC 1 cut(s) 1998
Eco53kI GAGCTC 1 cut(s) 1279
Eco57I CTGAAG 3 cut(s) 267, 663, 1143
Eco81I CCTNAGG 2 cut(s) 705, 894
EcoICRI GAGCTC 1 cut(s) 1279
EcoRI GAATTC 2 cut(s) 1682, 1769
EcoT14I CCWWGG 6 cut(s) 510, 1355, 1381, 1494, 1829, 2085
EcoT38I GRGCYC 2 cut(s) 1281, 2225
ErhI CCWWGG 6 cut(s) 510, 1355, 1381, 1494, 1829, 2085
FalI AAGNNNNNCTT 2 cut(s) 2106, 2138
FbaI TGATCA 1 cut(s) 2302
Fnu4HI GCNGC 4 cut(s) 180, 397, 1200, 1522
FokI GGATG 1 cut(s) 2057
FriOI GRGCYC 2 cut(s) 1281, 2225
Fsp4HI GCNGC 4 cut(s) 180, 397, 1200, 1522
FspBI CTAG 4 cut(s) 888, 1070, 1083, 1970
GluI GCNGC 4 cut(s) 180, 397, 1200, 1522
GsuI CTGGAG 2 cut(s) 249, 1521
HaeIII GGCC 1 cut(s) 1828
HapII CCGG 1 cut(s) 2353
HincII GTYRAC 2 cut(s) 1080, 2254
HindII GTYRAC 2 cut(s) 1080, 2254
HindIII AAGCTT 2 cut(s) 463, 586
HpaII CCGG 1 cut(s) 2353
HphI GGTGA 2 cut(s) 160, 526
Hpy166II GTNNAC 5 cut(s) 151, 1080, 1116, 2254, 2350
Hpy188III TCNNGA 9 cut(s) 213, 266, 361, 707, 773, 803, 1190, 1250, 1282
Hpy8I GTNNAC 5 cut(s) 151, 1080, 1116, 2254, 2350
HpyAV CCTTC 5 cut(s) 396, 1118, 1697, 1699, 2382
HpyCH4III ACNGT 7 cut(s) 1077, 1266, 1637, 1665, 1779, 1904, 2154
HpyCH4IV ACGT 2 cut(s) 207, 1744
HpyF10VI GCNNNNNNNGC 3 cut(s) 458, 581, 2039
HpyF3I CTNAG 5 cut(s) 198, 384, 705, 894, 2330
HpySE526I ACGT 2 cut(s) 207, 1744
Ksp22I TGATCA 1 cut(s) 2302
Kzo9I GATC 7 cut(s) 1216, 1963, 2060, 2089, 2119, 2302, 2368
LguI GCTCTTC 1 cut(s) 1059
LmnI GCTCC 2 cut(s) 172, 1276
Lsp1109I GCAGC 4 cut(s) 191, 408, 1211, 1508
LweI GCATC 4 cut(s) 232, 491, 1939, 2035
MaeI CTAG 4 cut(s) 888, 1070, 1083, 1970
MaeII ACGT 2 cut(s) 207, 1744
MaeIII GTNAC 2 cut(s) 544, 637
MalI GATC 7 cut(s) 1218, 1965, 2062, 2091, 2121, 2304, 2370
MboI GATC 7 cut(s) 1216, 1963, 2060, 2089, 2119, 2302, 2368
MfeI CAATTG 3 cut(s) 662, 761, 1953
MflI RGATCY 4 cut(s) 1216, 2089, 2119, 2368
MhlI GDGCHC 3 cut(s) 1281, 1791, 2225
MlyI GAGTC 6 cut(s) 209, 256, 488, 1121, 1337, 2075
MmeI TCCRAC 3 cut(s) 988, 1798, 2049
MroXI GAANNNNTTC 5 cut(s) 374, 558, 681, 1686, 1818
MseI TTAA 3 cut(s) 534, 1614, 2279
MspA1I CMGCKG 1 cut(s) 2042
MspI CCGG 1 cut(s) 2353
MunI CAATTG 3 cut(s) 662, 761, 1953
Mva1269I GAATGC 2 cut(s) 340, 1440
MwoI GCNNNNNNNGC 3 cut(s) 458, 581, 2039
NcoI CCATGG 3 cut(s) 510, 1355, 1381
NdeII GATC 7 cut(s) 1216, 1963, 2060, 2089, 2119, 2302, 2368
NlaIV GGNNCC 6 cut(s) 174, 508, 631, 1218, 2091, 2318
NmeAIII GCCGAG 2 cut(s) 445, 1789
NmuCI GTSAC 1 cut(s) 637
NspI RCATGY 3 cut(s) 431, 2287, 2345
PagI TCATGA 1 cut(s) 802
PciI ACATGT 2 cut(s) 2283, 2341
PciSI GCTCTTC 1 cut(s) 1059
PctI GAATGC 2 cut(s) 340, 1440
PdmI GAANNNNTTC 5 cut(s) 374, 558, 681, 1686, 1818
PkrI GCNGC 4 cut(s) 181, 398, 1201, 1523
PleI GAGTC 6 cut(s) 208, 256, 488, 1120, 1336, 2075
PpsI GAGTC 6 cut(s) 208, 256, 488, 1120, 1336, 2075
PscI ACATGT 2 cut(s) 2283, 2341
Psp124BI GAGCTC 1 cut(s) 1281
PspN4I GGNNCC 6 cut(s) 174, 508, 631, 1218, 2091, 2318
PspPI GGNCC 2 cut(s) 1826, 1998
PsuI RGATCY 4 cut(s) 1216, 2089, 2119, 2368
SacI GAGCTC 1 cut(s) 1281
SapI GCTCTTC 1 cut(s) 1059
SaqAI TTAA 3 cut(s) 534, 1614, 2279
SatI GCNGC 4 cut(s) 180, 397, 1200, 1522
Sau3AI GATC 7 cut(s) 1216, 1963, 2060, 2089, 2119, 2302, 2368
Sau96I GGNCC 2 cut(s) 1826, 1998
SchI GAGTC 6 cut(s) 209, 256, 488, 1121, 1337, 2075
SduI GDGCHC 3 cut(s) 1281, 1791, 2225
SfaNI GCATC 4 cut(s) 232, 491, 1939, 2035
SfcI CTRYAG 1 cut(s) 1423
SinI GGWCC 1 cut(s) 1998
SmlI CTYRAG 2 cut(s) 771, 1280
SmoI CTYRAG 2 cut(s) 771, 1280
SsiI CCGC 3 cut(s) 6, 1867, 2042
SspMI CTAG 4 cut(s) 888, 1070, 1083, 1970
SstI GAGCTC 1 cut(s) 1281
StyI CCWWGG 6 cut(s) 510, 1355, 1381, 1494, 1829, 2085
TaaI ACNGT 7 cut(s) 1077, 1266, 1637, 1665, 1779, 1904, 2154
TaiI ACGT 2 cut(s) 210, 1747
TaqI TCGA 9 cut(s) 126, 212, 360, 872, 1446, 1855, 2053, 2063, 2270
Tru1I TTAA 3 cut(s) 534, 1614, 2279
Tru9I TTAA 3 cut(s) 534, 1614, 2279
TscAI CASTG 3 cut(s) 1189, 1642, 1893
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 4 cut(s) 179, 396, 1199, 1521
Tsp45I GTSAC 1 cut(s) 637
TspGWI ACGGA 1 cut(s) 2351
TspRI CASTG 3 cut(s) 1189, 1642, 1893
VpaK11BI GGWCC 1 cut(s) 1998
XceI RCATGY 3 cut(s) 431, 2287, 2345
XmnI GAANNNNTTC 5 cut(s) 374, 558, 681, 1686, 1818
XspI CTAG 4 cut(s) 888, 1070, 1083, 1970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.