Rh3DG014900

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002986
Physical Location & Seq
Reverse (-)
886284 .. 888362
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG014900.1

Sequence Viewer

Length: 2079 bp
ATGATTGCTCAGTTTTCCGACTCCGTTGTGTTCAGATTCTTCAGCACACTCTTCGTCGAAGAAGAGATTTCTCCAATGAGGAATTCCGGTTTCTCTAGCACTGTACCGGAAAGAGCTGAATCTGAGATTCGGAAAAGTTACGAAGAAGATGATGATTCAGAATTCTTTGATGTTGGTGATATAGAGATCTCAGAGCCAAATTTCGTGTTTAAATTTGAGTACCAGACTGGGGAAATATTAAAAACACCGGAAAAAGCGGATGTAATTGGTGATTATGTAGCTTTTCAGGCCCAAAAGAATACTAGTAGTAGTTGCTTCATTGAAGAAGCAGAAAATGGGTCTGCTCAGAAATCAAATTGTGGTTCTATCGAAGCAACTGATGTTGATGAAGGAAAAAGTGCTCAGGGTTTTACAGAGGGTTTTGACCAATTGGTTAGAGAAGTACAAGTTCATGAGGAAAAAGGTACTGATCAGGGTTTTAGAGATGGTGCATTTGAGGAAGAGGAGGTAGAGAAGAAATCAGTGATTGAAGAAGATTCTGTTTCGGACGACGAAGAAGGATCCGAGAGTCAGGAAGAAGTAGATGTTAATAATGAAGAAATGTCTCATTCAGATGAGGAAAGTGATAGTACTGGTGATGAGAAACTGGCTACTAGTCATGAAGATGAGTTGGTTTCGGAAGAGAACTTTGTTAGTTCGGAATCTGATTCTGATTTGATTTGCTCAAGTCTAGATGATGATGAAGTAGGTGCTTTGGAAGACATTGGATTGCACGAGCCTGATGGTTTTGATGAAGAAGATGATGATATAATGGAACAAGTTAGAGAACTAGAAGAGTTGTGTACGACTGATGGTCAAAACTCGAAAGGGTCCGAACCTAAAGGAGAAGAAAAGCTAGTGGATGATTATAATGGTTCCGAGGATTCAGACACATTGGACGTTTTGTGGGAACACCAAGAGTTGATAGACCAGTTGAAAATGGAGCTCAAGACAGTCAGAGCTACAACTGGTCTGCCTACAATTTTTGAAGAATCCGAGTCTCTGGATATGGATGACTTGAAGCCACTCAAGATTGATGAAAAATACCAACACGGAGATAGAATGAGGGTGCTTCACAAGTTCTACAAGAGTTACAGGACTCGAATGCGCAAGTTGGATATTTTGAGTTACCAGAAGTCATATGCAATAGGTGTCCTGCAGTCTAAGAATCCACTTAGACCGTTTTCGAGCTACAAATCCTCAACTCCAGCAATCAAGTCCTTTCTTTCCAACTGGAGGCTATGTAGACCCAAAATAGATTGTGATCCAATTGTTAAGTTCATTAAGGATGTGCATAGTGACTTGGAATTGGTCTATGTAGGGCAGTTGTGCCTTTCATGGGAGTTCCTTCAATGGCAATATGAGAAGGCCTTGGAGCTATTGGAATCTGCCCCTAATCAAATACATTCATACAATGAAGTTGCAGAAGAATTGCAACATTTTCAAGTGCTCCTGCAAAGATTTATAGAGGATGAACATTTTCAAGGGGCAAGGGTCGAAAACTATGTTAAAAATCGATGTGCTATGCATAAGCTTCTACAAGTTCCAGTAATCAGAGAGGACAAATCGGAGGAAGGAAAGAAAGAGGATGCAATTACCACTGACAGTCTTGTCGAGATGTTGAGAGAATCGATAAGGACCATTTGGCGATTCATTCGAGCTGATAAAAATGCCAACATAACCACCGCTTGTCGAAAAAGATCACCTGATGGAGATTGCTTGGATCGAGAGCTTTTAGTGGAAGTTCAAGCAGATCTTCGGAAGAAAGAGAGGAAGCTTAAAGAGCTATTGAGGAGTGAGAACCGGATCATAAAGAGATTCCAAAAGCATGAAGAAGAAGGAGAAGAAGAGGAAGACAGCGGAGATCATGGTCACCATAACTTCTTTTCTCAAGTGGACATCAGATTAGTTTCAAGGGTTTTGAACATGTCAACTATAACACAAGAGCAACTAGTGTGGTGTCAAAGTAAGTTGAGCGAGATACACTTTGTGAAGCGCAAGATTCATGTAGATCCCTCTTTCTTGTTGTTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

692

Amino Acids

79.74

Weight (kDa)

4.52

Isoelectric Point (pI)

52.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 446 - 691 2.4e-88 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 909
AasI GACNNNNNNGTC 1 cut(s) 1649
Acc16I TGCGCA 1 cut(s) 1148
AccI GTMKAC 1 cut(s) 1285
AciI CCGC 3 cut(s) 257, 1725, 1899
AclWI GGATC 6 cut(s) 555, 568, 1298, 1770, 1853, 2045
AcsI RAATTY 4 cut(s) 82, 161, 199, 212
AcuI CTGAAG 1 cut(s) 25
AdeI CACNNNGTG 1 cut(s) 2029
AfaI GTAC 6 cut(s) 105, 221, 444, 466, 631, 844
AfiI CCNNNNNNNGG 3 cut(s) 229, 1275, 1378
AflIII ACRYGT 1 cut(s) 1965
AhdI GACNNNNNGTC 1 cut(s) 852
AhlI ACTAGT 3 cut(s) 302, 653, 1990
AjuI GAANNNNNNNTTGG 2 cut(s) 67, 99
Alw21I GWGCWC 3 cut(s) 403, 987, 1491
Alw26I GTCTC 2 cut(s) 609, 1044
AlwI GGATC 6 cut(s) 555, 568, 1298, 1770, 1853, 2045
AoxI GGCC 2 cut(s) 288, 1407
ApoI RAATTY 4 cut(s) 82, 161, 199, 212
Asp700I GAANNNNTTC 1 cut(s) 1518
AspLEI GCGC 2 cut(s) 1149, 2037
AspS9I GGNCC 3 cut(s) 289, 870, 1677
AsuHPI GGTGA 5 cut(s) 188, 281, 647, 1734, 1904
AvaII GGWCC 2 cut(s) 870, 1677
BamHI GGATCC 1 cut(s) 560
BanII GRGCYC 1 cut(s) 987
BauI CACGAG 1 cut(s) 773
BbsI GAAGAC 2 cut(s) 765, 1899
Bbv12I GWGCWC 3 cut(s) 403, 987, 1491
BccI CCATC 4 cut(s) 479, 776, 845, 1742
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BclI TGATCA 1 cut(s) 469
BcoDI GTCTC 2 cut(s) 609, 1044
BcuI ACTAGT 3 cut(s) 302, 653, 1990
BfaI CTAG 7 cut(s) 96, 303, 654, 731, 830, 896, 1991
BfmI CTRYAG 1 cut(s) 1196
BglII AGATCT 2 cut(s) 186, 1792
BmcAI AGTACT 1 cut(s) 631
Bme18I GGWCC 2 cut(s) 870, 1677
BmeRI GACNNNNNGTC 1 cut(s) 852
BmgT120I GGNCC 3 cut(s) 289, 870, 1677
BmiI GGNNCC 3 cut(s) 562, 871, 916
BmrI ACTGGG 1 cut(s) 237
BmsI GCATC 1 cut(s) 1618
BmuI ACTGGG 1 cut(s) 237
BpiI GAAGAC 2 cut(s) 765, 1899
BpmI CTGGAG 2 cut(s) 1230, 1294
Bpu10I CCTNAGC 1 cut(s) 402
BpuEI CTTGAG 4 cut(s) 709, 971, 1052, 1914
Bsa29I ATCGAT 2 cut(s) 1555, 1670
BsaBI GATNNNNATC 1 cut(s) 1302
BsaJI CCNNGG 2 cut(s) 918, 1410
BsaWI WCCGGW 4 cut(s) 86, 106, 247, 1842
BsaXI ACNNNNNCTCC 2 cut(s) 1267, 1297
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 1275, 1378
Bse1I ACTGG 7 cut(s) 232, 637, 651, 970, 1012, 1277, 1586
Bse8I GATNNNNATC 1 cut(s) 1302
BseCI ATCGAT 2 cut(s) 1555, 1670
BseDI CCNNGG 2 cut(s) 918, 1410
BseGI GGATG 6 cut(s) 265, 907, 1057, 1333, 1516, 1633
BseJI GATNNNNATC 1 cut(s) 1302
BseLI CCNNNNNNNGG 3 cut(s) 229, 1275, 1378
BseMII CTCAG 5 cut(s) 23, 114, 204, 359, 416
BseNI ACTGG 7 cut(s) 232, 637, 651, 970, 1012, 1277, 1586
BseRI GAGGAG 2 cut(s) 518, 1846
BshFI GGCC 2 cut(s) 290, 1409
BshVI ATCGAT 2 cut(s) 1555, 1670
BsiHKAI GWGCWC 3 cut(s) 403, 987, 1491
BsiSI CCGG 4 cut(s) 87, 107, 248, 1843
BslI CCNNNNNNNGG 3 cut(s) 229, 1275, 1378
BsmAI GTCTC 2 cut(s) 609, 1044
BsmI GAATGC 1 cut(s) 1149
BsnI GGCC 2 cut(s) 290, 1409
Bsp1286I GDGCHC 3 cut(s) 403, 987, 1491
BspACI CCGC 3 cut(s) 257, 1725, 1899
BspANI GGCC 2 cut(s) 290, 1409
BspCNI CTCAG 5 cut(s) 22, 115, 203, 358, 415
BspDI ATCGAT 2 cut(s) 1555, 1670
BspHI TCATGA 2 cut(s) 451, 658
BspLI GGNNCC 3 cut(s) 562, 871, 916
BspMAI CTGCAG 1 cut(s) 1200
BspPI GGATC 6 cut(s) 555, 568, 1298, 1770, 1853, 2045
BsrI ACTGG 7 cut(s) 232, 637, 651, 970, 1012, 1277, 1586
BssECI CCNNGG 2 cut(s) 918, 1410
BssSI CACGAG 1 cut(s) 773
BssT1I CCWWGG 1 cut(s) 1410
Bst2BI CACGAG 1 cut(s) 773
Bst4CI ACNGT 4 cut(s) 103, 994, 1221, 1646
Bst6I CTCTTC 6 cut(s) 56, 57, 495, 675, 828, 1881
BstDEI CTNAG 7 cut(s) 9, 123, 190, 345, 402, 1203, 1214
BstEII GGTNACC 1 cut(s) 1910
BstF5I GGATG 6 cut(s) 265, 907, 1057, 1333, 1516, 1633
BstHHI GCGC 2 cut(s) 1149, 2037
BstMAI GTCTC 2 cut(s) 609, 1044
BstMWI GCNNNNNNNGC 2 cut(s) 287, 1822
BstNSI RCATGY 1 cut(s) 1969
BstPI GGTNACC 1 cut(s) 1910
BstSFI CTRYAG 1 cut(s) 1196
BstV2I GAAGAC 2 cut(s) 765, 1899
BstX2I RGATCY 4 cut(s) 186, 560, 1792, 2050
BstYI RGATCY 4 cut(s) 186, 560, 1792, 2050
Bsu15I ATCGAT 2 cut(s) 1555, 1670
BsuRI GGCC 2 cut(s) 290, 1409
BsuTUI ATCGAT 2 cut(s) 1555, 1670
BtsCI GGATG 6 cut(s) 265, 907, 1057, 1333, 1516, 1633
BtsIMutI CAGTG 3 cut(s) 99, 528, 1638
CciI TCATGA 2 cut(s) 451, 658
CfoI GCGC 2 cut(s) 1149, 2037
Cfr13I GGNCC 3 cut(s) 289, 870, 1677
ClaI ATCGAT 2 cut(s) 1555, 1670
Csp6I GTAC 6 cut(s) 104, 220, 443, 465, 630, 843
CspCI CAANNNNNGTGG 2 cut(s) 1976, 2011
CviAII CATG 8 cut(s) 452, 659, 1377, 1868, 1907, 1966, 2045, 2073
CviQI GTAC 6 cut(s) 104, 220, 443, 465, 630, 843
DdeI CTNAG 7 cut(s) 9, 123, 190, 345, 402, 1203, 1214
DraI TTTAAA 1 cut(s) 211
DraIII CACNNNGTG 1 cut(s) 2029
DrdI GACNNNNNNGTC 1 cut(s) 1649
DriI GACNNNNNGTC 1 cut(s) 852
DseDI GACNNNNNNGTC 1 cut(s) 1649
Eam1104I CTCTTC 6 cut(s) 56, 57, 495, 675, 828, 1881
Eam1105I GACNNNNNGTC 1 cut(s) 852
EarI CTCTTC 6 cut(s) 56, 57, 495, 675, 828, 1881
Ecl136II GAGCTC 1 cut(s) 985
Eco130I CCWWGG 1 cut(s) 1410
Eco147I AGGCCT 1 cut(s) 1409
Eco24I GRGCYC 1 cut(s) 987
Eco47I GGWCC 2 cut(s) 870, 1677
Eco53kI GAGCTC 1 cut(s) 985
Eco57I CTGAAG 1 cut(s) 25
Eco91I GGTNACC 1 cut(s) 1910
EcoICRI GAGCTC 1 cut(s) 985
EcoO65I GGTNACC 1 cut(s) 1910
EcoRI GAATTC 2 cut(s) 82, 161
EcoT14I CCWWGG 1 cut(s) 1410
EcoT22I ATGCAT 1 cut(s) 1569
EcoT38I GRGCYC 1 cut(s) 987
ErhI CCWWGG 1 cut(s) 1410
FaeI CATG 8 cut(s) 455, 662, 1380, 1871, 1910, 1969, 2048, 2076
FalI AAGNNNNNCTT 2 cut(s) 1779, 1811
FatI CATG 8 cut(s) 451, 658, 1376, 1867, 1906, 1965, 2044, 2072
FauNDI CATATG 1 cut(s) 1180
FbaI TGATCA 1 cut(s) 469
FblI GTMKAC 1 cut(s) 1285
FokI GGATG 6 cut(s) 272, 914, 1064, 1340, 1523, 1640
FriOI GRGCYC 1 cut(s) 987
FspBI CTAG 7 cut(s) 96, 303, 654, 731, 830, 896, 1991
FspI TGCGCA 1 cut(s) 1148
GlaI GCGC 2 cut(s) 1148, 2036
GsuI CTGGAG 2 cut(s) 1230, 1294
HaeIII GGCC 2 cut(s) 290, 1409
HapII CCGG 4 cut(s) 87, 107, 248, 1843
HhaI GCGC 2 cut(s) 1149, 2037
Hin1II CATG 8 cut(s) 455, 662, 1380, 1871, 1910, 1969, 2048, 2076
Hin6I GCGC 2 cut(s) 1147, 2035
HinP1I GCGC 2 cut(s) 1147, 2035
HincII GTYRAC 1 cut(s) 1971
HindII GTYRAC 1 cut(s) 1971
HindIII AAGCTT 2 cut(s) 1571, 1814
HpaII CCGG 4 cut(s) 87, 107, 248, 1843
HphI GGTGA 5 cut(s) 188, 281, 647, 1734, 1904
Hpy166II GTNNAC 4 cut(s) 843, 1286, 1936, 1971
Hpy188III TCNNGA 9 cut(s) 452, 572, 659, 731, 988, 1043, 1069, 1654, 1766
Hpy8I GTNNAC 4 cut(s) 843, 1286, 1936, 1971
Hpy99I CGWCG 2 cut(s) 59, 554
HpyAV CCTTC 6 cut(s) 383, 551, 1397, 1399, 1607, 1871
HpyCH4III ACNGT 4 cut(s) 103, 994, 1221, 1646
HpyCH4IV ACGT 1 cut(s) 939
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 1822
HpyF3I CTNAG 7 cut(s) 9, 123, 190, 345, 402, 1203, 1214
HpySE526I ACGT 1 cut(s) 939
Hsp92II CATG 8 cut(s) 455, 662, 1380, 1871, 1910, 1969, 2048, 2076
HspAI GCGC 2 cut(s) 1147, 2035
Ksp22I TGATCA 1 cut(s) 469
LmnI GCTCC 3 cut(s) 982, 1414, 1494
LweI GCATC 1 cut(s) 1618
MaeI CTAG 7 cut(s) 96, 303, 654, 731, 830, 896, 1991
MaeII ACGT 1 cut(s) 939
MaeIII GTNAC 5 cut(s) 137, 1130, 1166, 1337, 1910
MfeI CAATTG 2 cut(s) 428, 1308
MflI RGATCY 4 cut(s) 186, 560, 1792, 2050
MhlI GDGCHC 3 cut(s) 403, 987, 1491
MlyI GAGTC 4 cut(s) 14, 577, 1046, 1132
MmeI TCCRAC 3 cut(s) 42, 1134, 1293
Mph1103I ATGCAT 1 cut(s) 1569
MroXI GAANNNNTTC 1 cut(s) 1518
MseI TTAA 8 cut(s) 210, 239, 588, 1314, 1323, 1548, 1818, 2077
MslI CAYNNNNRTG 2 cut(s) 612, 663
MspA1I CMGCKG 1 cut(s) 1899
MspI CCGG 4 cut(s) 87, 107, 248, 1843
MunI CAATTG 2 cut(s) 428, 1308
Mva1269I GAATGC 1 cut(s) 1149
MwoI GCNNNNNNNGC 2 cut(s) 287, 1822
NdeI CATATG 1 cut(s) 1180
NlaIII CATG 8 cut(s) 455, 662, 1380, 1871, 1910, 1969, 2048, 2076
NlaIV GGNNCC 3 cut(s) 562, 871, 916
NmuCI GTSAC 2 cut(s) 1337, 1910
NsbI TGCGCA 1 cut(s) 1148
NsiI ATGCAT 1 cut(s) 1569
NspI RCATGY 1 cut(s) 1969
PagI TCATGA 2 cut(s) 451, 658
PceI AGGCCT 1 cut(s) 1409
PciI ACATGT 1 cut(s) 1965
PctI GAATGC 1 cut(s) 1149
PdmI GAANNNNTTC 1 cut(s) 1518
PleI GAGTC 4 cut(s) 14, 576, 1045, 1132
PpsI GAGTC 4 cut(s) 14, 576, 1045, 1132
PscI ACATGT 1 cut(s) 1965
PsiI TTATAA 1 cut(s) 909
Psp124BI GAGCTC 1 cut(s) 987
PspEI GGTNACC 1 cut(s) 1910
PspN4I GGNNCC 3 cut(s) 562, 871, 916
PspPI GGNCC 3 cut(s) 289, 870, 1677
PstI CTGCAG 1 cut(s) 1200
PsuI RGATCY 4 cut(s) 186, 560, 1792, 2050
RsaI GTAC 6 cut(s) 105, 221, 444, 466, 631, 844
RsaNI GTAC 6 cut(s) 104, 220, 443, 465, 630, 843
RseI CAYNNNNRTG 2 cut(s) 612, 663
SacI GAGCTC 1 cut(s) 987
SaqAI TTAA 8 cut(s) 210, 239, 588, 1314, 1323, 1548, 1818, 2077
Sau96I GGNCC 3 cut(s) 289, 870, 1677
ScaI AGTACT 1 cut(s) 631
SchI GAGTC 4 cut(s) 14, 577, 1046, 1132
SduI GDGCHC 3 cut(s) 403, 987, 1491
SfaNI GCATC 1 cut(s) 1618
SfcI CTRYAG 1 cut(s) 1196
SinI GGWCC 2 cut(s) 870, 1677
SmiMI CAYNNNNRTG 2 cut(s) 612, 663
SmlI CTYRAG 4 cut(s) 724, 986, 1067, 1929
SmoI CTYRAG 4 cut(s) 724, 986, 1067, 1929
SpeI ACTAGT 3 cut(s) 302, 653, 1990
SseBI AGGCCT 1 cut(s) 1409
SsiI CCGC 3 cut(s) 257, 1725, 1899
SspI AATATT 1 cut(s) 237
SspMI CTAG 7 cut(s) 96, 303, 654, 731, 830, 896, 1991
SstI GAGCTC 1 cut(s) 987
StuI AGGCCT 1 cut(s) 1409
StyI CCWWGG 1 cut(s) 1410
TaaI ACNGT 4 cut(s) 103, 994, 1221, 1646
TaiI ACGT 1 cut(s) 942
TatI WGTACW 2 cut(s) 442, 629
Tru1I TTAA 8 cut(s) 210, 239, 588, 1314, 1323, 1548, 1818, 2077
Tru9I TTAA 8 cut(s) 210, 239, 588, 1314, 1323, 1548, 1818, 2077
TscAI CASTG 3 cut(s) 106, 528, 1645
TseFI GTSAC 2 cut(s) 1337, 1910
Tsp45I GTSAC 2 cut(s) 1337, 1910
TspGWI ACGGA 2 cut(s) 13, 1107
TspRI CASTG 3 cut(s) 106, 528, 1645
VpaK11BI GGWCC 2 cut(s) 870, 1677
XapI RAATTY 4 cut(s) 82, 161, 199, 212
XbaI TCTAGA 1 cut(s) 730
XceI RCATGY 1 cut(s) 1969
XmiI GTMKAC 1 cut(s) 1285
XmnI GAANNNNTTC 1 cut(s) 1518
XspI CTAG 7 cut(s) 96, 303, 654, 731, 830, 896, 1991
ZrmI AGTACT 1 cut(s) 631
Zsp2I ATGCAT 1 cut(s) 1569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.