Rw3G001080

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002986
Physical Location & Seq
Reverse (-)
853276 .. 856060
2785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001080.1

Sequence Viewer

Length: 2166 bp
ATGTCTTTATGTTACATTTTATATGCTTTATTTAATAACATTTCCCATATTAAATCCCTCATTTTCCATCTCTCCCAATACCCACAAATGATTGCTCAGTTTTCCGACTCCGTTGTGTTCAGATTCTTCAGCACACTCTTCGTCGAAGAAGAGATTTCTCCAATGAGGAATTCGGGTTTCTCTAGCACTGTACCGGAAAGAGCTGAATCTGAGATTCGGAAAAGTTACGAAGAAGATGATAATGATGAATTCTTTGATGTTGGTGATATAGAGATCTCAGAGCCAAATTTCGTGTTTAAATTTGAGTACCAGACTTGGGAAATATTAAAAACACCGGAAAAAGCGGATGTAATTGGTGATTATGTAGCTTTTCAGGCCCAAAAGAATACTAGTAGTAGTTGCTTCATTGAAGAAGCAGAAAATGGGTCTGCTCAGAAATCAAATTGTGGTTCTATCGAAGCAACTGATGTTGATGAAGGAAAAAGTGCTCAGGGTTTTACAGAGGGTTTTGACCAATTGGTTAGAGAAGTACAAGTTCATGAGGAAAAAGGTACTGATCAGGGTTTTGGAGATGGTGCATTTGAGGAAGAGGAGGTAGAGAAGAAATCAGTGATTGAAGAAGATTCTGTTTCGGACGACGAAGAAGGATCGGAGAGTCAGGAAGAAGTAGATGTTAATAATGAAGAAATGTCTCATTCAGATGAGGAAAGTGATAGTACTGATGATGAGAAACTGGCTGCTAGTCATGAAGATGAGTTGGTTTCGGAAGAGAACTTTGTTAGTTCGGAATCTGATTCTGATTTGATTTGCTCAAGTCTAGATGAGGATGAAGTAGGTGCTTTGGAAGACATTGGATTGCACGAGCCTGATGGTTTTGATGAAGAAGATGATGATATAATGGAACAAGTTAGAGAACTAGAAGAGTTGTGTACGACTGATGGTCAAAACTCGAAAGGGTCCGAACCTAAAGGAGAAGAAAAGCTAGTGGATGATTATAATGGTTCAGAGGATTCAGACACATTGGATGTTTTGTGGGAACACCAAGAGTTGATAGACCAGTTGAAAATGGAGCTCAAGACAGTCAGAGCTACAGCTGGTCTGCCTACAATTTTTGAAGAATCCGAGTCTCTGGATATGGATGACTTGAAGCCACTCAAGATTGATGAAAAATACCAACACGGAGATAGAATGAGGGTGCTTCACAAGTTCTACAAGAGGTACAGGACTCGAATGCGCAAGTTGGATATTTTGAGTTACCAGAAGTCATATGCAATAGGTGTCCTGCAGTCTAAGAATCCACTTAGATCGTTTTCGAGCTACAAATCCTCAACTCCAGCAATCAAGTCCTTTCTTTCCAACTGGAGGCTATGTAGACCCAAAATAGATTGTGATCCAATTGTTAAGTTCATTAAGGATGTGCATAGTGACTTGGAATTGGTCTATGTAGGGCAGTTGTGCCTTTCATGGGAGTTCCTTAAATGGCAATATGAGAAGGCCTTGGAGCTATTGGAATCTGCCCCTAATCAAATACATTCATACAATGAAGTTGCAGAAGAATTGCAACATTTTCAAGTGCTCCTGCAAAGATTTATAGAGGATGAACATTTTCAAGGGGCAAGGGTCGAAAACTACGTTAAAAATCGATGTGCTATGCACAAACTTCTACAAGTTCCAGTAATCAGAGAGGACAAATCGGAGGAAGGAAAGAAAGAGGATGCAATTACCACTGACAGTCTTGTCGAGATGTTGAGAGAATCGATAAGGACCATTTGGCGATTCATTCGAGCTGATAAAAATGCCAACATAACCACCGCTTGTCGAAAAAGATCACTTGATGGAGATTGCTTGGATCCAGAGCTTTTAGTGGAAGTTCAAGCAGATCTTCGGAAGAAAGAGAGGAAGCTTAAAGAGCTATTGAGGAGTGAGAACCGGATCATAAAGAGATTCCAAAAGCATGAAGAAGGAGGAGAAGAAGAGGAAGACAGCGGAGATCATGGTCACCATAACTTCTTTTCTCAAGTGGACATCAGATTAGTTTCAAGGGTTTTGAACATGTCAACTATAACACAAGAGCAACTAGTGTGGTGTCAAAGTAAGTTGAGCGAGATACACTTTGTGAAGCGCAAGATTCATGTAGATCCCTCTTTCTTGTTGTTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

721

Amino Acids

83.19

Weight (kDa)

4.56

Isoelectric Point (pI)

52.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 475 - 720 7.2e-88 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 996
AasI GACNNNNNNGTC 1 cut(s) 1736
Acc16I TGCGCA 1 cut(s) 1235
AccI GTMKAC 1 cut(s) 1372
AciI CCGC 3 cut(s) 344, 1812, 1986
AclWI GGATC 6 cut(s) 655, 1385, 1844, 1857, 1940, 2132
AcsI RAATTY 4 cut(s) 169, 248, 286, 299
AcuI CTGAAG 1 cut(s) 112
AdeI CACNNNGTG 1 cut(s) 2116
AfaI GTAC 7 cut(s) 192, 308, 531, 553, 718, 931, 1220
AfiI CCNNNNNNNGG 3 cut(s) 316, 1362, 1465
AflIII ACRYGT 1 cut(s) 2052
AhdI GACNNNNNGTC 1 cut(s) 939
AhlI ACTAGT 2 cut(s) 389, 2077
AjuI GAANNNNNNNTTGG 2 cut(s) 154, 186
Alw21I GWGCWC 3 cut(s) 490, 1074, 1578
Alw26I GTCTC 2 cut(s) 696, 1131
AlwI GGATC 6 cut(s) 655, 1385, 1844, 1857, 1940, 2132
AoxI GGCC 2 cut(s) 375, 1494
ApeKI GCWGC 1 cut(s) 737
ApoI RAATTY 4 cut(s) 169, 248, 286, 299
Asp700I GAANNNNTTC 1 cut(s) 1605
AspLEI GCGC 2 cut(s) 1236, 2124
AspS9I GGNCC 3 cut(s) 376, 957, 1764
AsuHPI GGTGA 3 cut(s) 275, 368, 1991
AvaII GGWCC 2 cut(s) 957, 1764
BamHI GGATCC 1 cut(s) 1849
BanII GRGCYC 1 cut(s) 1074
BauI CACGAG 1 cut(s) 860
BbsI GAAGAC 2 cut(s) 852, 1986
Bbv12I GWGCWC 3 cut(s) 490, 1074, 1578
BbvI GCAGC 1 cut(s) 724
BccI CCATC 5 cut(s) 75, 566, 863, 932, 1829
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BclI TGATCA 1 cut(s) 556
BcoDI GTCTC 2 cut(s) 696, 1131
BcuI ACTAGT 2 cut(s) 389, 2077
BfaI CTAG 7 cut(s) 183, 390, 741, 818, 917, 983, 2078
BfmI CTRYAG 2 cut(s) 1089, 1283
BglII AGATCT 2 cut(s) 273, 1879
BisI GCNGC 1 cut(s) 738
BlsI GCNGC 1 cut(s) 739
BmcAI AGTACT 1 cut(s) 718
Bme18I GGWCC 2 cut(s) 957, 1764
BmeRI GACNNNNNGTC 1 cut(s) 939
BmgT120I GGNCC 3 cut(s) 376, 957, 1764
BmiI GGNNCC 2 cut(s) 958, 1851
BmsI GCATC 1 cut(s) 1705
BpiI GAAGAC 2 cut(s) 852, 1986
BpmI CTGGAG 2 cut(s) 1317, 1381
Bpu10I CCTNAGC 1 cut(s) 489
BpuEI CTTGAG 4 cut(s) 796, 1058, 1139, 2001
Bsa29I ATCGAT 2 cut(s) 1642, 1757
BsaBI GATNNNNATC 1 cut(s) 1389
BsaJI CCNNGG 1 cut(s) 1497
BsaWI WCCGGW 3 cut(s) 193, 334, 1929
BsaXI ACNNNNNCTCC 2 cut(s) 1354, 1384
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 1362, 1465
Bse1I ACTGG 4 cut(s) 738, 1057, 1364, 1673
Bse8I GATNNNNATC 1 cut(s) 1389
BseCI ATCGAT 2 cut(s) 1642, 1757
BseDI CCNNGG 1 cut(s) 1497
BseGI GGATG 8 cut(s) 352, 832, 994, 1030, 1144, 1420, 1603, 1720
BseJI GATNNNNATC 1 cut(s) 1389
BseLI CCNNNNNNNGG 3 cut(s) 316, 1362, 1465
BseMII CTCAG 5 cut(s) 110, 201, 291, 446, 503
BseNI ACTGG 4 cut(s) 738, 1057, 1364, 1673
BseRI GAGGAG 3 cut(s) 605, 1933, 1980
BseXI GCAGC 1 cut(s) 724
BshFI GGCC 2 cut(s) 377, 1496
BshVI ATCGAT 2 cut(s) 1642, 1757
BsiHKAI GWGCWC 3 cut(s) 490, 1074, 1578
BsiSI CCGG 3 cut(s) 194, 335, 1930
BslI CCNNNNNNNGG 3 cut(s) 316, 1362, 1465
BsmAI GTCTC 2 cut(s) 696, 1131
BsmI GAATGC 1 cut(s) 1236
BsnI GGCC 2 cut(s) 377, 1496
Bsp1286I GDGCHC 3 cut(s) 490, 1074, 1578
BspACI CCGC 3 cut(s) 344, 1812, 1986
BspANI GGCC 2 cut(s) 377, 1496
BspCNI CTCAG 5 cut(s) 109, 202, 290, 445, 502
BspDI ATCGAT 2 cut(s) 1642, 1757
BspHI TCATGA 2 cut(s) 538, 745
BspLI GGNNCC 2 cut(s) 958, 1851
BspMAI CTGCAG 1 cut(s) 1287
BspPI GGATC 6 cut(s) 655, 1385, 1844, 1857, 1940, 2132
BsrI ACTGG 4 cut(s) 738, 1057, 1364, 1673
BssECI CCNNGG 1 cut(s) 1497
BssSI CACGAG 1 cut(s) 860
BssT1I CCWWGG 1 cut(s) 1497
Bst2BI CACGAG 1 cut(s) 860
Bst4CI ACNGT 3 cut(s) 190, 1081, 1733
Bst6I CTCTTC 6 cut(s) 143, 144, 582, 762, 915, 1968
BstDEI CTNAG 7 cut(s) 96, 210, 277, 432, 489, 1290, 1301
BstEII GGTNACC 1 cut(s) 1997
BstF5I GGATG 8 cut(s) 352, 832, 994, 1030, 1144, 1420, 1603, 1720
BstHHI GCGC 2 cut(s) 1236, 2124
BstMAI GTCTC 2 cut(s) 696, 1131
BstMWI GCNNNNNNNGC 2 cut(s) 374, 1909
BstNSI RCATGY 1 cut(s) 2056
BstPI GGTNACC 1 cut(s) 1997
BstSFI CTRYAG 2 cut(s) 1089, 1283
BstV1I GCAGC 1 cut(s) 724
BstV2I GAAGAC 2 cut(s) 852, 1986
BstX2I RGATCY 4 cut(s) 273, 1849, 1879, 2137
BstYI RGATCY 4 cut(s) 273, 1849, 1879, 2137
Bsu15I ATCGAT 2 cut(s) 1642, 1757
BsuRI GGCC 2 cut(s) 377, 1496
BsuTUI ATCGAT 2 cut(s) 1642, 1757
BtsCI GGATG 8 cut(s) 352, 832, 994, 1030, 1144, 1420, 1603, 1720
BtsIMutI CAGTG 3 cut(s) 186, 615, 1725
CciI TCATGA 2 cut(s) 538, 745
CfoI GCGC 2 cut(s) 1236, 2124
Cfr13I GGNCC 3 cut(s) 376, 957, 1764
ClaI ATCGAT 2 cut(s) 1642, 1757
Csp6I GTAC 7 cut(s) 191, 307, 530, 552, 717, 930, 1219
CspCI CAANNNNNGTGG 2 cut(s) 2063, 2098
CviAII CATG 8 cut(s) 539, 746, 1464, 1955, 1994, 2053, 2132, 2160
CviQI GTAC 7 cut(s) 191, 307, 530, 552, 717, 930, 1219
DdeI CTNAG 7 cut(s) 96, 210, 277, 432, 489, 1290, 1301
DraI TTTAAA 1 cut(s) 298
DraIII CACNNNGTG 1 cut(s) 2116
DrdI GACNNNNNNGTC 1 cut(s) 1736
DriI GACNNNNNGTC 1 cut(s) 939
DseDI GACNNNNNNGTC 1 cut(s) 1736
Eam1104I CTCTTC 6 cut(s) 143, 144, 582, 762, 915, 1968
Eam1105I GACNNNNNGTC 1 cut(s) 939
EarI CTCTTC 6 cut(s) 143, 144, 582, 762, 915, 1968
Ecl136II GAGCTC 1 cut(s) 1072
Eco130I CCWWGG 1 cut(s) 1497
Eco147I AGGCCT 1 cut(s) 1496
Eco24I GRGCYC 1 cut(s) 1074
Eco47I GGWCC 2 cut(s) 957, 1764
Eco53kI GAGCTC 1 cut(s) 1072
Eco57I CTGAAG 1 cut(s) 112
Eco91I GGTNACC 1 cut(s) 1997
EcoICRI GAGCTC 1 cut(s) 1072
EcoO65I GGTNACC 1 cut(s) 1997
EcoRI GAATTC 2 cut(s) 169, 248
EcoT14I CCWWGG 1 cut(s) 1497
EcoT38I GRGCYC 1 cut(s) 1074
ErhI CCWWGG 1 cut(s) 1497
FaeI CATG 8 cut(s) 542, 749, 1467, 1958, 1997, 2056, 2135, 2163
FalI AAGNNNNNCTT 2 cut(s) 1866, 1898
FatI CATG 8 cut(s) 538, 745, 1463, 1954, 1993, 2052, 2131, 2159
FauNDI CATATG 1 cut(s) 1267
FbaI TGATCA 1 cut(s) 556
FblI GTMKAC 1 cut(s) 1372
Fnu4HI GCNGC 1 cut(s) 738
FokI GGATG 8 cut(s) 359, 839, 1001, 1037, 1151, 1427, 1610, 1727
FriOI GRGCYC 1 cut(s) 1074
Fsp4HI GCNGC 1 cut(s) 738
FspBI CTAG 7 cut(s) 183, 390, 741, 818, 917, 983, 2078
FspI TGCGCA 1 cut(s) 1235
GlaI GCGC 2 cut(s) 1235, 2123
GluI GCNGC 1 cut(s) 738
GsuI CTGGAG 2 cut(s) 1317, 1381
HaeIII GGCC 2 cut(s) 377, 1496
HapII CCGG 3 cut(s) 194, 335, 1930
HhaI GCGC 2 cut(s) 1236, 2124
Hin1II CATG 8 cut(s) 542, 749, 1467, 1958, 1997, 2056, 2135, 2163
Hin6I GCGC 2 cut(s) 1234, 2122
HinP1I GCGC 2 cut(s) 1234, 2122
HincII GTYRAC 1 cut(s) 2058
HindII GTYRAC 1 cut(s) 2058
HindIII AAGCTT 1 cut(s) 1901
HpaII CCGG 3 cut(s) 194, 335, 1930
HphI GGTGA 3 cut(s) 275, 368, 1991
Hpy166II GTNNAC 4 cut(s) 930, 1373, 2023, 2058
Hpy188III TCNNGA 9 cut(s) 539, 659, 746, 818, 1075, 1130, 1156, 1741, 1853
Hpy8I GTNNAC 4 cut(s) 930, 1373, 2023, 2058
Hpy99I CGWCG 2 cut(s) 146, 641
HpyAV CCTTC 5 cut(s) 470, 638, 1486, 1694, 1955
HpyCH4III ACNGT 3 cut(s) 190, 1081, 1733
HpyCH4IV ACGT 1 cut(s) 1632
HpyF10VI GCNNNNNNNGC 2 cut(s) 374, 1909
HpyF3I CTNAG 7 cut(s) 96, 210, 277, 432, 489, 1290, 1301
HpySE526I ACGT 1 cut(s) 1632
Hsp92II CATG 8 cut(s) 542, 749, 1467, 1958, 1997, 2056, 2135, 2163
HspAI GCGC 2 cut(s) 1234, 2122
Ksp22I TGATCA 1 cut(s) 556
LmnI GCTCC 3 cut(s) 1069, 1501, 1581
Lsp1109I GCAGC 1 cut(s) 724
LweI GCATC 1 cut(s) 1705
MaeI CTAG 7 cut(s) 183, 390, 741, 818, 917, 983, 2078
MaeII ACGT 1 cut(s) 1632
MaeIII GTNAC 5 cut(s) 11, 224, 1253, 1424, 1997
MfeI CAATTG 2 cut(s) 515, 1395
MflI RGATCY 4 cut(s) 273, 1849, 1879, 2137
MhlI GDGCHC 3 cut(s) 490, 1074, 1578
MlyI GAGTC 4 cut(s) 101, 664, 1133, 1219
MmeI TCCRAC 3 cut(s) 129, 1221, 1380
MroXI GAANNNNTTC 1 cut(s) 1605
MslI CAYNNNNRTG 2 cut(s) 699, 750
MspA1I CMGCKG 2 cut(s) 1094, 1986
MspI CCGG 3 cut(s) 194, 335, 1930
MunI CAATTG 2 cut(s) 515, 1395
Mva1269I GAATGC 1 cut(s) 1236
MwoI GCNNNNNNNGC 2 cut(s) 374, 1909
NdeI CATATG 1 cut(s) 1267
NlaIII CATG 8 cut(s) 542, 749, 1467, 1958, 1997, 2056, 2135, 2163
NlaIV GGNNCC 2 cut(s) 958, 1851
NmuCI GTSAC 2 cut(s) 1424, 1997
NsbI TGCGCA 1 cut(s) 1235
NspI RCATGY 1 cut(s) 2056
PagI TCATGA 2 cut(s) 538, 745
PceI AGGCCT 1 cut(s) 1496
PciI ACATGT 1 cut(s) 2052
PcsI WCGNNNNNNNCGW 1 cut(s) 1629
PctI GAATGC 1 cut(s) 1236
PdmI GAANNNNTTC 1 cut(s) 1605
PkrI GCNGC 1 cut(s) 739
PleI GAGTC 4 cut(s) 101, 663, 1132, 1219
PpsI GAGTC 4 cut(s) 101, 663, 1132, 1219
PscI ACATGT 1 cut(s) 2052
PsiI TTATAA 1 cut(s) 996
Psp124BI GAGCTC 1 cut(s) 1074
PspEI GGTNACC 1 cut(s) 1997
PspN4I GGNNCC 2 cut(s) 958, 1851
PspPI GGNCC 3 cut(s) 376, 957, 1764
PstI CTGCAG 1 cut(s) 1287
PsuI RGATCY 4 cut(s) 273, 1849, 1879, 2137
PvuII CAGCTG 1 cut(s) 1094
RsaI GTAC 7 cut(s) 192, 308, 531, 553, 718, 931, 1220
RsaNI GTAC 7 cut(s) 191, 307, 530, 552, 717, 930, 1219
RseI CAYNNNNRTG 2 cut(s) 699, 750
SacI GAGCTC 1 cut(s) 1074
SatI GCNGC 1 cut(s) 738
Sau96I GGNCC 3 cut(s) 376, 957, 1764
ScaI AGTACT 1 cut(s) 718
SchI GAGTC 4 cut(s) 101, 664, 1133, 1219
SduI GDGCHC 3 cut(s) 490, 1074, 1578
SfaNI GCATC 1 cut(s) 1705
SfcI CTRYAG 2 cut(s) 1089, 1283
SinI GGWCC 2 cut(s) 957, 1764
SmiMI CAYNNNNRTG 2 cut(s) 699, 750
SmlI CTYRAG 4 cut(s) 811, 1073, 1154, 2016
SmoI CTYRAG 4 cut(s) 811, 1073, 1154, 2016
SpeI ACTAGT 2 cut(s) 389, 2077
SseBI AGGCCT 1 cut(s) 1496
SsiI CCGC 3 cut(s) 344, 1812, 1986
SspI AATATT 1 cut(s) 324
SspMI CTAG 7 cut(s) 183, 390, 741, 818, 917, 983, 2078
SstI GAGCTC 1 cut(s) 1074
StuI AGGCCT 1 cut(s) 1496
StyI CCWWGG 1 cut(s) 1497
TaaI ACNGT 3 cut(s) 190, 1081, 1733
TaiI ACGT 1 cut(s) 1635
TatI WGTACW 2 cut(s) 529, 716
TscAI CASTG 3 cut(s) 193, 615, 1732
TseFI GTSAC 2 cut(s) 1424, 1997
TseI GCWGC 1 cut(s) 737
Tsp45I GTSAC 2 cut(s) 1424, 1997
TspGWI ACGGA 2 cut(s) 100, 1194
TspRI CASTG 3 cut(s) 193, 615, 1732
VpaK11BI GGWCC 2 cut(s) 957, 1764
XapI RAATTY 4 cut(s) 169, 248, 286, 299
XbaI TCTAGA 1 cut(s) 817
XceI RCATGY 1 cut(s) 2056
XmiI GTMKAC 1 cut(s) 1372
XmnI GAANNNNTTC 1 cut(s) 1605
XspI CTAG 7 cut(s) 183, 390, 741, 818, 917, 983, 2078
ZrmI AGTACT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.