RchiOBHm_Chr3g0448731

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002986
Physical Location & Seq
Reverse (-)
833986 .. 836076
2091 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41615

Sequence Viewer

Length: 2091 bp
ATGATTGCTCAGTTTTCCGACTCCGTTGTGTTCAGATTCTTCAGCACACTCTTCGTCGAAGAAGAGATTTCTCCGATGAGGAATTCCGGTTTCTCTAGCACTGTACCGGAAAGAGCTGAATCTGAGATTCGGAAAAGTTACGAAGAAGATGATGATGATGATGATGATTCAGAATTCTTTGATGTTGGTGATATAGAGATCTCAGAGCCAAATTTCGTGTTTAAATTTGAGTACCAGACTTGGGAAATATTAAAAACACCGGAAAAAGCGGATGTAATTGGTGATTATGTAGCTTTTCAGGCCCAAAAGAATACTAGTAGTAGTTGCTTCATTGAAGAATCAGAAAATGGGTCTGCTCAGAAATCAAATTGTGGTTCTATCGAAGCAACTGATGTTGATGAAGGAAAAAGTGCTCAGGGTTTTACCGAGGGTTTTGACCAATTGGTTAGAGAAGTACAAGTTCATGAGGAAAAAGGTGCTGATCAGGGTTTTGGAGATGGTGCATTTGAGGAAGAGGAGGTAGAGAAGAAATCAGTGATTGAAGAAGATTCTGTTTCGGACGACGAAGAAGGATCGGAGAGTCAGGAAGAAGTAGATGTTAATAATGAAGAAATGTCTCATTCAGATGAGGAAAGTGATAGTACTGGTGATGAGAAACTGGCTACTAGTCATGAAGATGAGTTGGTTTCGGAAGAGAACTTTGTTAGTTCGGAATCTGATTCTGATTTGATTTGCTCAAGTCTAGATGAGGATGAAGTAGGTGCTTTGGAAGACATTGGATTGCACGAGCCTGATGGTTTTGATGAAGAAGATGATGATATAATGGAACAAGTTAGAGAACTAGAAGAGTTGTGTACGACTGATGGTCAAAACTCGAAAGGGTCCGAACCTAAAGGAGAAGAAAAGCTAGTGGATGATTATAATGGTTCCGAGGATTCAGACACATTGGATGTTTTGTGGGAACACCAAGAGTTGATAGACCAGTTGAAAATGGAGCTCAAGACAGTCAGAGCTACAACTGGTCTGCCTACAATTTTTGAAGAATCCGAGTCTCTGGATATGGATGACTTGAAGCCACTCAAGATTGATGAAAAATACCAACACGGAGATAGAATGAGGGTGCTTCACAAGTTCTACAAGAGTTACAGGACTCGAATGCGCAAGTTGGATATTTTGAGTTACCAGAAGTCATATGCAATAGGTGTCCTGCAGTCTAAGAATCCACTTAGACCGTTTTCGAGCTACAAATCCTCAACTCCAGCAATCAAGTCCTTTCTTTCCAACTGGAGGCTATGTAGACCCAAAATAGATTGTGATCCAATTGTTAAGTTCATTAAGGATGTGCATAGTGACTTGGAATTGGTCTATGTAGGGCAGTTGTGCCTTTCATGGGAGTTCCTTCAATGGCAATATGAGAAGGCCTTGGAGCTATTGGAATCTGCCCCTAATCAAATACATTCATACAATGAAGTTGCAGAAGAATTGCAACATTTTCAAGTGCTCCTGCAAAGATTTATAGAGGATGAGCATTTTCAAGGGGCAAGGGTCGAAAACTACGTTAAAAATCGATGTGCTATGCATAAACTTCTACAAGTTCCAGTAATCAGAGAGGACAAATCGGAGGAAGGAAAGAAAGAGGATGCAATTACCACTGACAGTCTTGTCGAGATGTTGAGAGAATCGATAAGGACCATTTGGCGATTCATTCGAGCTGATAAAAATGCCAACATAACCACCGCTTGTCGAAAAAGATCACTTGATGGAGATTGCTTGGATCCAGAGCTTTTAGTGGAAGTTCAAGCAGATCTTCGGAAGAAAGAGAGGAAGCTTAAAGAGCTATTGAGGAGTGAGAACCGGATCATAAAGAGATTCCAAAAGCATGAAGAAGGAGGAGAAGAAGAGGAAGACAGCGGAGATCATGGTCACCATAACTTCTTTTCTCAAGTGGACATCAGATTAGTTTCAAGGGTTTTGAACATGTCAACTATAACACAAGAGCAACTAGTGTGGTGTCAAAGTAAGTTGAGCGAGATACACTTTGTGAAGCGCAAGATTCATGTAGATCCCTCTTTCTTGTTGTTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

696

Amino Acids

80.11

Weight (kDa)

4.47

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 450 - 695 4.6e-88 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 921
AasI GACNNNNNNGTC 1 cut(s) 1661
Acc16I TGCGCA 1 cut(s) 1160
AccI GTMKAC 1 cut(s) 1297
AciI CCGC 3 cut(s) 269, 1737, 1911
AclWI GGATC 6 cut(s) 580, 1310, 1769, 1782, 1865, 2057
AcsI RAATTY 4 cut(s) 82, 173, 211, 224
AcuI CTGAAG 1 cut(s) 25
AdeI CACNNNGTG 1 cut(s) 2041
AfaI GTAC 5 cut(s) 105, 233, 456, 643, 856
AfiI CCNNNNNNNGG 3 cut(s) 241, 1287, 1390
AflIII ACRYGT 1 cut(s) 1977
AhdI GACNNNNNGTC 1 cut(s) 864
AhlI ACTAGT 3 cut(s) 314, 665, 2002
Alw21I GWGCWC 3 cut(s) 415, 999, 1503
Alw26I GTCTC 2 cut(s) 621, 1056
AlwI GGATC 6 cut(s) 580, 1310, 1769, 1782, 1865, 2057
AoxI GGCC 2 cut(s) 300, 1419
ApoI RAATTY 4 cut(s) 82, 173, 211, 224
AspLEI GCGC 2 cut(s) 1161, 2049
AspS9I GGNCC 3 cut(s) 301, 882, 1689
AsuHPI GGTGA 4 cut(s) 200, 293, 659, 1916
AvaII GGWCC 2 cut(s) 882, 1689
BamHI GGATCC 1 cut(s) 1774
BanII GRGCYC 1 cut(s) 999
BauI CACGAG 1 cut(s) 785
BbsI GAAGAC 2 cut(s) 777, 1911
Bbv12I GWGCWC 3 cut(s) 415, 999, 1503
BccI CCATC 4 cut(s) 491, 788, 857, 1754
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BclI TGATCA 1 cut(s) 481
BcoDI GTCTC 2 cut(s) 621, 1056
BcuI ACTAGT 3 cut(s) 314, 665, 2002
BfaI CTAG 7 cut(s) 96, 315, 666, 743, 842, 908, 2003
BfmI CTRYAG 1 cut(s) 1208
BglII AGATCT 2 cut(s) 198, 1804
BmcAI AGTACT 1 cut(s) 643
Bme18I GGWCC 2 cut(s) 882, 1689
BmeRI GACNNNNNGTC 1 cut(s) 864
BmgT120I GGNCC 3 cut(s) 301, 882, 1689
BmiI GGNNCC 3 cut(s) 883, 928, 1776
BmsI GCATC 1 cut(s) 1630
BpiI GAAGAC 2 cut(s) 777, 1911
BpmI CTGGAG 2 cut(s) 1242, 1306
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 4 cut(s) 721, 983, 1064, 1926
Bsa29I ATCGAT 2 cut(s) 1567, 1682
BsaBI GATNNNNATC 1 cut(s) 1314
BsaJI CCNNGG 3 cut(s) 426, 930, 1422
BsaWI WCCGGW 4 cut(s) 86, 106, 259, 1854
BsaXI ACNNNNNCTCC 2 cut(s) 1279, 1309
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 1287, 1390
Bse1I ACTGG 6 cut(s) 649, 663, 982, 1024, 1289, 1598
Bse8I GATNNNNATC 1 cut(s) 1314
BseCI ATCGAT 2 cut(s) 1567, 1682
BseDI CCNNGG 3 cut(s) 426, 930, 1422
BseGI GGATG 8 cut(s) 277, 757, 919, 955, 1069, 1345, 1528, 1645
BseJI GATNNNNATC 1 cut(s) 1314
BseLI CCNNNNNNNGG 3 cut(s) 241, 1287, 1390
BseMII CTCAG 5 cut(s) 23, 114, 216, 371, 428
BseNI ACTGG 6 cut(s) 649, 663, 982, 1024, 1289, 1598
BseRI GAGGAG 3 cut(s) 530, 1858, 1905
BshFI GGCC 2 cut(s) 302, 1421
BshVI ATCGAT 2 cut(s) 1567, 1682
BsiHKAI GWGCWC 3 cut(s) 415, 999, 1503
BsiSI CCGG 4 cut(s) 87, 107, 260, 1855
BslI CCNNNNNNNGG 3 cut(s) 241, 1287, 1390
BsmAI GTCTC 2 cut(s) 621, 1056
BsmI GAATGC 1 cut(s) 1161
BsnI GGCC 2 cut(s) 302, 1421
Bsp1286I GDGCHC 3 cut(s) 415, 999, 1503
BspACI CCGC 3 cut(s) 269, 1737, 1911
BspANI GGCC 2 cut(s) 302, 1421
BspCNI CTCAG 5 cut(s) 22, 115, 215, 370, 427
BspDI ATCGAT 2 cut(s) 1567, 1682
BspHI TCATGA 2 cut(s) 463, 670
BspLI GGNNCC 3 cut(s) 883, 928, 1776
BspMAI CTGCAG 1 cut(s) 1212
BspPI GGATC 6 cut(s) 580, 1310, 1769, 1782, 1865, 2057
BsrI ACTGG 6 cut(s) 649, 663, 982, 1024, 1289, 1598
BssECI CCNNGG 3 cut(s) 426, 930, 1422
BssSI CACGAG 1 cut(s) 785
BssT1I CCWWGG 1 cut(s) 1422
Bst2BI CACGAG 1 cut(s) 785
Bst4CI ACNGT 4 cut(s) 103, 1006, 1233, 1658
Bst6I CTCTTC 6 cut(s) 56, 57, 507, 687, 840, 1893
BstDEI CTNAG 7 cut(s) 9, 123, 202, 357, 414, 1215, 1226
BstEII GGTNACC 1 cut(s) 1922
BstF5I GGATG 8 cut(s) 277, 757, 919, 955, 1069, 1345, 1528, 1645
BstHHI GCGC 2 cut(s) 1161, 2049
BstMAI GTCTC 2 cut(s) 621, 1056
BstMWI GCNNNNNNNGC 2 cut(s) 299, 1834
BstNSI RCATGY 1 cut(s) 1981
BstPI GGTNACC 1 cut(s) 1922
BstSFI CTRYAG 1 cut(s) 1208
BstV2I GAAGAC 2 cut(s) 777, 1911
BstX2I RGATCY 4 cut(s) 198, 1774, 1804, 2062
BstYI RGATCY 4 cut(s) 198, 1774, 1804, 2062
Bsu15I ATCGAT 2 cut(s) 1567, 1682
BsuRI GGCC 2 cut(s) 302, 1421
BsuTUI ATCGAT 2 cut(s) 1567, 1682
BtsCI GGATG 8 cut(s) 277, 757, 919, 955, 1069, 1345, 1528, 1645
BtsIMutI CAGTG 3 cut(s) 99, 540, 1650
CciI TCATGA 2 cut(s) 463, 670
CfoI GCGC 2 cut(s) 1161, 2049
Cfr13I GGNCC 3 cut(s) 301, 882, 1689
ClaI ATCGAT 2 cut(s) 1567, 1682
Csp6I GTAC 5 cut(s) 104, 232, 455, 642, 855
CspCI CAANNNNNGTGG 2 cut(s) 1988, 2023
CviAII CATG 8 cut(s) 464, 671, 1389, 1880, 1919, 1978, 2057, 2085
CviQI GTAC 5 cut(s) 104, 232, 455, 642, 855
DdeI CTNAG 7 cut(s) 9, 123, 202, 357, 414, 1215, 1226
DraI TTTAAA 1 cut(s) 223
DraIII CACNNNGTG 1 cut(s) 2041
DrdI GACNNNNNNGTC 1 cut(s) 1661
DriI GACNNNNNGTC 1 cut(s) 864
DseDI GACNNNNNNGTC 1 cut(s) 1661
Eam1104I CTCTTC 6 cut(s) 56, 57, 507, 687, 840, 1893
Eam1105I GACNNNNNGTC 1 cut(s) 864
EarI CTCTTC 6 cut(s) 56, 57, 507, 687, 840, 1893
Ecl136II GAGCTC 1 cut(s) 997
Eco130I CCWWGG 1 cut(s) 1422
Eco147I AGGCCT 1 cut(s) 1421
Eco24I GRGCYC 1 cut(s) 999
Eco47I GGWCC 2 cut(s) 882, 1689
Eco53kI GAGCTC 1 cut(s) 997
Eco57I CTGAAG 1 cut(s) 25
Eco91I GGTNACC 1 cut(s) 1922
EcoICRI GAGCTC 1 cut(s) 997
EcoO65I GGTNACC 1 cut(s) 1922
EcoRI GAATTC 2 cut(s) 82, 173
EcoT14I CCWWGG 1 cut(s) 1422
EcoT22I ATGCAT 1 cut(s) 1581
EcoT38I GRGCYC 1 cut(s) 999
ErhI CCWWGG 1 cut(s) 1422
FaeI CATG 8 cut(s) 467, 674, 1392, 1883, 1922, 1981, 2060, 2088
FalI AAGNNNNNCTT 2 cut(s) 1791, 1823
FatI CATG 8 cut(s) 463, 670, 1388, 1879, 1918, 1977, 2056, 2084
FauNDI CATATG 1 cut(s) 1192
FbaI TGATCA 1 cut(s) 481
FblI GTMKAC 1 cut(s) 1297
FokI GGATG 8 cut(s) 284, 764, 926, 962, 1076, 1352, 1535, 1652
FriOI GRGCYC 1 cut(s) 999
FspBI CTAG 7 cut(s) 96, 315, 666, 743, 842, 908, 2003
FspI TGCGCA 1 cut(s) 1160
GlaI GCGC 2 cut(s) 1160, 2048
GsuI CTGGAG 2 cut(s) 1242, 1306
HaeIII GGCC 2 cut(s) 302, 1421
HapII CCGG 4 cut(s) 87, 107, 260, 1855
HhaI GCGC 2 cut(s) 1161, 2049
Hin1II CATG 8 cut(s) 467, 674, 1392, 1883, 1922, 1981, 2060, 2088
Hin6I GCGC 2 cut(s) 1159, 2047
HinP1I GCGC 2 cut(s) 1159, 2047
HincII GTYRAC 1 cut(s) 1983
HindII GTYRAC 1 cut(s) 1983
HindIII AAGCTT 1 cut(s) 1826
HpaII CCGG 4 cut(s) 87, 107, 260, 1855
HphI GGTGA 4 cut(s) 200, 293, 659, 1916
Hpy166II GTNNAC 4 cut(s) 855, 1298, 1948, 1983
Hpy188III TCNNGA 9 cut(s) 464, 584, 671, 743, 1000, 1055, 1081, 1666, 1778
Hpy8I GTNNAC 4 cut(s) 855, 1298, 1948, 1983
Hpy99I CGWCG 2 cut(s) 59, 566
HpyAV CCTTC 6 cut(s) 395, 563, 1409, 1411, 1619, 1880
HpyCH4III ACNGT 4 cut(s) 103, 1006, 1233, 1658
HpyCH4IV ACGT 1 cut(s) 1557
HpyF10VI GCNNNNNNNGC 2 cut(s) 299, 1834
HpyF3I CTNAG 7 cut(s) 9, 123, 202, 357, 414, 1215, 1226
HpySE526I ACGT 1 cut(s) 1557
Hsp92II CATG 8 cut(s) 467, 674, 1392, 1883, 1922, 1981, 2060, 2088
HspAI GCGC 2 cut(s) 1159, 2047
Ksp22I TGATCA 1 cut(s) 481
LmnI GCTCC 3 cut(s) 994, 1426, 1506
LweI GCATC 1 cut(s) 1630
MaeI CTAG 7 cut(s) 96, 315, 666, 743, 842, 908, 2003
MaeII ACGT 1 cut(s) 1557
MaeIII GTNAC 5 cut(s) 137, 1142, 1178, 1349, 1922
MfeI CAATTG 2 cut(s) 440, 1320
MflI RGATCY 4 cut(s) 198, 1774, 1804, 2062
MhlI GDGCHC 3 cut(s) 415, 999, 1503
MlyI GAGTC 4 cut(s) 14, 589, 1058, 1144
MmeI TCCRAC 3 cut(s) 42, 1146, 1305
Mph1103I ATGCAT 1 cut(s) 1581
MseI TTAA 8 cut(s) 222, 251, 600, 1326, 1335, 1560, 1830, 2089
MslI CAYNNNNRTG 2 cut(s) 624, 675
MspA1I CMGCKG 1 cut(s) 1911
MspI CCGG 4 cut(s) 87, 107, 260, 1855
MunI CAATTG 2 cut(s) 440, 1320
Mva1269I GAATGC 1 cut(s) 1161
MwoI GCNNNNNNNGC 2 cut(s) 299, 1834
NdeI CATATG 1 cut(s) 1192
NlaIII CATG 8 cut(s) 467, 674, 1392, 1883, 1922, 1981, 2060, 2088
NlaIV GGNNCC 3 cut(s) 883, 928, 1776
NmuCI GTSAC 2 cut(s) 1349, 1922
NsbI TGCGCA 1 cut(s) 1160
NsiI ATGCAT 1 cut(s) 1581
NspI RCATGY 1 cut(s) 1981
PagI TCATGA 2 cut(s) 463, 670
PceI AGGCCT 1 cut(s) 1421
PciI ACATGT 1 cut(s) 1977
PcsI WCGNNNNNNNCGW 1 cut(s) 1554
PctI GAATGC 1 cut(s) 1161
PleI GAGTC 4 cut(s) 14, 588, 1057, 1144
PpsI GAGTC 4 cut(s) 14, 588, 1057, 1144
PscI ACATGT 1 cut(s) 1977
PsiI TTATAA 1 cut(s) 921
Psp124BI GAGCTC 1 cut(s) 999
PspEI GGTNACC 1 cut(s) 1922
PspN4I GGNNCC 3 cut(s) 883, 928, 1776
PspPI GGNCC 3 cut(s) 301, 882, 1689
PstI CTGCAG 1 cut(s) 1212
PsuI RGATCY 4 cut(s) 198, 1774, 1804, 2062
RsaI GTAC 5 cut(s) 105, 233, 456, 643, 856
RsaNI GTAC 5 cut(s) 104, 232, 455, 642, 855
RseI CAYNNNNRTG 2 cut(s) 624, 675
SacI GAGCTC 1 cut(s) 999
SaqAI TTAA 8 cut(s) 222, 251, 600, 1326, 1335, 1560, 1830, 2089
Sau96I GGNCC 3 cut(s) 301, 882, 1689
ScaI AGTACT 1 cut(s) 643
SchI GAGTC 4 cut(s) 14, 589, 1058, 1144
SduI GDGCHC 3 cut(s) 415, 999, 1503
SfaNI GCATC 1 cut(s) 1630
SfcI CTRYAG 1 cut(s) 1208
SinI GGWCC 2 cut(s) 882, 1689
SmiMI CAYNNNNRTG 2 cut(s) 624, 675
SmlI CTYRAG 4 cut(s) 736, 998, 1079, 1941
SmoI CTYRAG 4 cut(s) 736, 998, 1079, 1941
SpeI ACTAGT 3 cut(s) 314, 665, 2002
SseBI AGGCCT 1 cut(s) 1421
SsiI CCGC 3 cut(s) 269, 1737, 1911
SspI AATATT 1 cut(s) 249
SspMI CTAG 7 cut(s) 96, 315, 666, 743, 842, 908, 2003
SstI GAGCTC 1 cut(s) 999
StuI AGGCCT 1 cut(s) 1421
StyI CCWWGG 1 cut(s) 1422
TaaI ACNGT 4 cut(s) 103, 1006, 1233, 1658
TaiI ACGT 1 cut(s) 1560
TatI WGTACW 2 cut(s) 454, 641
Tru1I TTAA 8 cut(s) 222, 251, 600, 1326, 1335, 1560, 1830, 2089
Tru9I TTAA 8 cut(s) 222, 251, 600, 1326, 1335, 1560, 1830, 2089
TscAI CASTG 3 cut(s) 106, 540, 1657
TseFI GTSAC 2 cut(s) 1349, 1922
Tsp45I GTSAC 2 cut(s) 1349, 1922
TspGWI ACGGA 2 cut(s) 13, 1119
TspRI CASTG 3 cut(s) 106, 540, 1657
VpaK11BI GGWCC 2 cut(s) 882, 1689
XapI RAATTY 4 cut(s) 82, 173, 211, 224
XbaI TCTAGA 1 cut(s) 742
XceI RCATGY 1 cut(s) 1981
XmiI GTMKAC 1 cut(s) 1297
XspI CTAG 7 cut(s) 96, 315, 666, 743, 842, 908, 2003
ZrmI AGTACT 1 cut(s) 643
Zsp2I ATGCAT 1 cut(s) 1581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.