RLG00000025876

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002986
Physical Location & Seq
Forward (+)
48877191 .. 48879278
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025876

Sequence Viewer

Length: 2088 bp
ATGATTGCTCAGTTTTCCAACTCCGTTGTGTTCAGATTCTTCAGCACTCTCTTCGTCGAAGAAGAGATTTCTCCGATGAGGAATTCCGGTTTCTCTAGCACTGTACCGGAAAGAGCTGAATCTGAGATTTGGAAAAGTTACGAAGAAGATGATGATGATGATGATGATGATTCAGAATTCTTTGATGTTGGTGATATAGAGATCTCAGAGCCAAATTTCGTGTTTAAATTTGAGTACCAGACTTGGGAAATATTAAAAACACCGGAAAAAGCGGATGTAATTGGTGATTATGTAGCTTTTCAGGCCCAAAAGAATACTAGCAGTTGCTTCATTGAAGAAGCAGAAAATGGGTCTGCTCAGAAATCAAATTGTGGTTCTATCGAAGCAACTGATGTTGATGAAGGAAAAAGCCCTCAGGGTTTTACAGAGGGTTTTGACCAATTGGTTGGAGAAGTACAAGTTCATGAGGAAAAAGGTGATCAGGGTTTTAGAGATGGTGCATTTGAGGAAGAGGAGGTAGAGAAGAAATCAGTGATTGAAGAAGATTCTGTTTCCGACGACAAAGAAGGATCCGAGAGTCAGGAAGAAGTAGAAGTTAATAATGAAGAAATGTCTCATTCAGATGAGGAAAGTGATAGTACCGGTGATGAGAAACTGGCTACTAGTCATGAAGATGAGTTGGTTTCGGAAGAGAACTTTGTTAGTTCGGAATCTGATTCTGATTTGATTTGCTCAAGTCTAGATGATGATGAAGTAGGTGCTTTGGAAGACATTGGATTGCACGAGCCTGATGGTTTTGATGAAGAAGATGATGATATAATGGAACAAGTTAGAGAACTAGAAGAGTTGTGTACGACTGATGATCAAAACTCGAAAGGGTCCGAACCTAAAGGAGAAGAAAAGCTAGTGGGTGACTATAATGGTTCCGAGGATTCAGACACATTGGAGGTTTTGTGGGAACATCAAGAGTTGATAGACCAGTTGAAAATGGAGCTCAAGAAAGTCAGAGCTACAACTGGTCTGCCTACAATTTTTGAAGAATCCGAGTCTCTGGATATGGATGACTTGAAGCCGCTCAAGATTGATGAAAAATACCAACACGGAGATAGAATGAGGGTGCTTCACAAGTTCTACAAGAGTTACAGGACTCGAATGCACAAGTTGGATATTTTGAATTACCAGAAGTTATATGCAATAGGTGTTCTGCAGTCTAAGAATCCACTTGGATCGTTTTCGAGCTACAAATCCTCAACTCCAGCAATCAAATCCTTTCTTTCCAACTGGAGGCTATGTAGACCTAAAATAGATTGTGATCCAATTGTTAAGTTCATTAAGGATGTGCATAGTGAATTGGAATTGGTCTATGTAGGGCAGTTGTGCCTTTCATGGGAGTTCCTTCAGTGGCAATATGAGAAGGCCTTGGAGCTATTGGAATCTGCCCCTAATCAAATACATTCATACAATGAAGTTGCAGAAGAATTGCAACATTTTCAAGTGCTCCTGCAAAGATTTATAGAGGATGAACATTTTCAAGGGGCAAGGGTCGAAAACTATGTTAAAAATAGACTTGCTATGCATAAGCTTCTACGAGTTCCAGTAATCAGAGAGGACAAATCGGAGGAAGGAAAGAAAGAGGATGCAATTACCACTGACAGTCTTGTCGAGATGTTGAGAGAATCGATAAGGACCTTTTGGCGATTCATTCGAGCTGATAAAAATGCCAACATAACCACCGCTTGTCGAAAAAGATCACTTGATGGAGATTGCTTGGATCCAGAGCTTTTAGTGGAAGTTCAAGCAGATCTTCGGAAGAAAGAGAGGAAGCTTAAAGAGCTATTGAGGAGTGAGAACCGGATCATAAAGAGATTCCAAAAGCATGAAGAAGAAGGAGAAGAAGAGGAAGACAGCGGAGATTATGGTCACCATAACTTCTTTTCTCAAGTGGACATCAGATTAGTTTCAAGGGTTTTGAACATGTCAACTATAACACAAGAGCAACTAGTGTGGTGTCAAAGTAAGTTGAGCGAGATACACTTTGTGAAGCGCAAGATTCATGTAGATCCCTCTTTCTTGTTGTTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

696

Amino Acids

80.26

Weight (kDa)

4.48

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 449 - 694 1.7e-87 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1658
AccBSI CCGCTC 1 cut(s) 1075
AccI GTMKAC 1 cut(s) 1294
AciI CCGC 4 cut(s) 272, 1073, 1734, 1908
AclWI GGATC 8 cut(s) 564, 577, 1234, 1307, 1766, 1779, 1862, 2054
AcsI RAATTY 4 cut(s) 82, 176, 214, 227
AcuI CTGAAG 2 cut(s) 25, 1382
AdeI CACNNNGTG 1 cut(s) 2038
AfaI GTAC 5 cut(s) 105, 236, 456, 640, 853
AfiI CCNNNNNNNGG 3 cut(s) 244, 1284, 1387
AflIII ACRYGT 1 cut(s) 1974
AgeI ACCGGT 1 cut(s) 641
AhlI ACTAGT 2 cut(s) 662, 1999
Alw21I GWGCWC 2 cut(s) 996, 1500
Alw26I GTCTC 2 cut(s) 618, 1053
AlwI GGATC 8 cut(s) 564, 577, 1234, 1307, 1766, 1779, 1862, 2054
AoxI GGCC 2 cut(s) 303, 1416
ApoI RAATTY 4 cut(s) 82, 176, 214, 227
AsiGI ACCGGT 1 cut(s) 641
Asp700I GAANNNNTTC 1 cut(s) 1527
AspLEI GCGC 1 cut(s) 2046
AspS9I GGNCC 3 cut(s) 304, 879, 1686
AsuHPI GGTGA 6 cut(s) 203, 296, 488, 656, 923, 1913
AvaII GGWCC 2 cut(s) 879, 1686
AxyI CCTNAGG 1 cut(s) 414
BamHI GGATCC 2 cut(s) 569, 1771
BanII GRGCYC 1 cut(s) 996
BauI CACGAG 1 cut(s) 782
BbsI GAAGAC 2 cut(s) 774, 1908
Bbv12I GWGCWC 2 cut(s) 996, 1500
BccI CCATC 3 cut(s) 488, 785, 1751
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BclI TGATCA 2 cut(s) 478, 862
BcoDI GTCTC 2 cut(s) 618, 1053
BcuI ACTAGT 2 cut(s) 662, 1999
BfaI CTAG 7 cut(s) 96, 318, 663, 740, 839, 905, 2000
BfmI CTRYAG 1 cut(s) 1205
BglII AGATCT 2 cut(s) 201, 1801
BisI GCNGC 1 cut(s) 1073
BlsI GCNGC 1 cut(s) 1074
Bme18I GGWCC 2 cut(s) 879, 1686
BmgT120I GGNCC 3 cut(s) 304, 879, 1686
BmiI GGNNCC 4 cut(s) 571, 880, 925, 1773
BmsI GCATC 1 cut(s) 1627
BpiI GAAGAC 2 cut(s) 774, 1908
BpmI CTGGAG 2 cut(s) 1239, 1303
BpuEI CTTGAG 4 cut(s) 718, 980, 1061, 1923
Bsa29I ATCGAT 1 cut(s) 1679
BsaBI GATNNNNATC 1 cut(s) 1311
BsaJI CCNNGG 2 cut(s) 927, 1419
BsaWI WCCGGW 5 cut(s) 86, 106, 262, 641, 1851
BsaXI ACNNNNNCTCC 4 cut(s) 938, 968, 1276, 1306
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 1284, 1387
Bse118I RCCGGY 1 cut(s) 641
Bse1I ACTGG 5 cut(s) 660, 979, 1021, 1286, 1595
Bse21I CCTNAGG 1 cut(s) 414
Bse8I GATNNNNATC 1 cut(s) 1311
BseCI ATCGAT 1 cut(s) 1679
BseDI CCNNGG 2 cut(s) 927, 1419
BseGI GGATG 5 cut(s) 280, 1066, 1342, 1525, 1642
BseJI GATNNNNATC 1 cut(s) 1311
BseLI CCNNNNNNNGG 3 cut(s) 244, 1284, 1387
BseMII CTCAG 5 cut(s) 23, 114, 219, 371, 428
BseNI ACTGG 5 cut(s) 660, 979, 1021, 1286, 1595
BseRI GAGGAG 2 cut(s) 527, 1855
BshFI GGCC 2 cut(s) 305, 1418
BshTI ACCGGT 1 cut(s) 641
BshVI ATCGAT 1 cut(s) 1679
BsiHKAI GWGCWC 2 cut(s) 996, 1500
BsiSI CCGG 5 cut(s) 87, 107, 263, 642, 1852
BslI CCNNNNNNNGG 3 cut(s) 244, 1284, 1387
BsmAI GTCTC 2 cut(s) 618, 1053
BsmI GAATGC 1 cut(s) 1158
BsnI GGCC 2 cut(s) 305, 1418
Bsp1286I GDGCHC 2 cut(s) 996, 1500
BspACI CCGC 4 cut(s) 272, 1073, 1734, 1908
BspANI GGCC 2 cut(s) 305, 1418
BspCNI CTCAG 5 cut(s) 22, 115, 218, 370, 427
BspDI ATCGAT 1 cut(s) 1679
BspHI TCATGA 2 cut(s) 463, 667
BspLI GGNNCC 4 cut(s) 571, 880, 925, 1773
BspMAI CTGCAG 1 cut(s) 1209
BspPI GGATC 8 cut(s) 564, 577, 1234, 1307, 1766, 1779, 1862, 2054
BsrBI CCGCTC 1 cut(s) 1075
BsrFI RCCGGY 1 cut(s) 641
BsrI ACTGG 5 cut(s) 660, 979, 1021, 1286, 1595
BssAI RCCGGY 1 cut(s) 641
BssECI CCNNGG 2 cut(s) 927, 1419
BssSI CACGAG 1 cut(s) 782
BssT1I CCWWGG 1 cut(s) 1419
Bst2BI CACGAG 1 cut(s) 782
Bst4CI ACNGT 2 cut(s) 103, 1655
Bst6I CTCTTC 6 cut(s) 56, 57, 504, 684, 837, 1890
BstDEI CTNAG 6 cut(s) 9, 123, 205, 357, 414, 1212
BstEII GGTNACC 1 cut(s) 1919
BstF5I GGATG 5 cut(s) 280, 1066, 1342, 1525, 1642
BstHHI GCGC 1 cut(s) 2046
BstMAI GTCTC 2 cut(s) 618, 1053
BstMWI GCNNNNNNNGC 2 cut(s) 302, 1831
BstNSI RCATGY 1 cut(s) 1978
BstPI GGTNACC 1 cut(s) 1919
BstSFI CTRYAG 1 cut(s) 1205
BstV2I GAAGAC 2 cut(s) 774, 1908
BstX2I RGATCY 5 cut(s) 201, 569, 1771, 1801, 2059
BstXI CCANNNNNNTGG 1 cut(s) 446
BstYI RGATCY 5 cut(s) 201, 569, 1771, 1801, 2059
Bsu15I ATCGAT 1 cut(s) 1679
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 2 cut(s) 305, 1418
BsuTUI ATCGAT 1 cut(s) 1679
BtsCI GGATG 5 cut(s) 280, 1066, 1342, 1525, 1642
BtsIMutI CAGTG 4 cut(s) 99, 537, 1406, 1647
CciI TCATGA 2 cut(s) 463, 667
CfoI GCGC 1 cut(s) 2046
Cfr10I RCCGGY 1 cut(s) 641
Cfr13I GGNCC 3 cut(s) 304, 879, 1686
ClaI ATCGAT 1 cut(s) 1679
Csp6I GTAC 5 cut(s) 104, 235, 455, 639, 852
CspAI ACCGGT 1 cut(s) 641
CspCI CAANNNNNGTGG 2 cut(s) 1985, 2020
CviAII CATG 7 cut(s) 464, 668, 1386, 1877, 1975, 2054, 2082
CviQI GTAC 5 cut(s) 104, 235, 455, 639, 852
DdeI CTNAG 6 cut(s) 9, 123, 205, 357, 414, 1212
DraI TTTAAA 1 cut(s) 226
DraIII CACNNNGTG 1 cut(s) 2038
DrdI GACNNNNNNGTC 1 cut(s) 1658
DseDI GACNNNNNNGTC 1 cut(s) 1658
Eam1104I CTCTTC 6 cut(s) 56, 57, 504, 684, 837, 1890
EarI CTCTTC 6 cut(s) 56, 57, 504, 684, 837, 1890
Ecl136II GAGCTC 1 cut(s) 994
Eco130I CCWWGG 1 cut(s) 1419
Eco147I AGGCCT 1 cut(s) 1418
Eco24I GRGCYC 1 cut(s) 996
Eco47I GGWCC 2 cut(s) 879, 1686
Eco53kI GAGCTC 1 cut(s) 994
Eco57I CTGAAG 2 cut(s) 25, 1382
Eco81I CCTNAGG 1 cut(s) 414
Eco91I GGTNACC 1 cut(s) 1919
EcoICRI GAGCTC 1 cut(s) 994
EcoO109I RGGNCCY 1 cut(s) 1686
EcoO65I GGTNACC 1 cut(s) 1919
EcoRI GAATTC 2 cut(s) 82, 176
EcoT14I CCWWGG 1 cut(s) 1419
EcoT22I ATGCAT 1 cut(s) 1578
EcoT38I GRGCYC 1 cut(s) 996
ErhI CCWWGG 1 cut(s) 1419
FaeI CATG 7 cut(s) 467, 671, 1389, 1880, 1978, 2057, 2085
FalI AAGNNNNNCTT 2 cut(s) 1788, 1820
FatI CATG 7 cut(s) 463, 667, 1385, 1876, 1974, 2053, 2081
FbaI TGATCA 2 cut(s) 478, 862
FblI GTMKAC 1 cut(s) 1294
Fnu4HI GCNGC 1 cut(s) 1073
FokI GGATG 5 cut(s) 287, 1073, 1349, 1532, 1649
FriOI GRGCYC 1 cut(s) 996
Fsp4HI GCNGC 1 cut(s) 1073
FspBI CTAG 7 cut(s) 96, 318, 663, 740, 839, 905, 2000
GlaI GCGC 1 cut(s) 2045
GluI GCNGC 1 cut(s) 1073
GsuI CTGGAG 2 cut(s) 1239, 1303
HaeIII GGCC 2 cut(s) 305, 1418
HapII CCGG 5 cut(s) 87, 107, 263, 642, 1852
HhaI GCGC 1 cut(s) 2046
Hin1II CATG 7 cut(s) 467, 671, 1389, 1880, 1978, 2057, 2085
Hin6I GCGC 1 cut(s) 2044
HinP1I GCGC 1 cut(s) 2044
HincII GTYRAC 1 cut(s) 1980
HindII GTYRAC 1 cut(s) 1980
HindIII AAGCTT 2 cut(s) 1580, 1823
HpaII CCGG 5 cut(s) 87, 107, 263, 642, 1852
HphI GGTGA 6 cut(s) 203, 296, 488, 656, 923, 1913
Hpy166II GTNNAC 4 cut(s) 852, 1295, 1945, 1980
Hpy8I GTNNAC 4 cut(s) 852, 1295, 1945, 1980
Hpy99I CGWCG 2 cut(s) 59, 560
HpyAV CCTTC 6 cut(s) 395, 560, 1406, 1408, 1616, 1880
HpyCH4III ACNGT 2 cut(s) 103, 1655
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 1831
HpyF3I CTNAG 6 cut(s) 9, 123, 205, 357, 414, 1212
Hsp92II CATG 7 cut(s) 467, 671, 1389, 1880, 1978, 2057, 2085
HspAI GCGC 1 cut(s) 2044
Ksp22I TGATCA 2 cut(s) 478, 862
LmnI GCTCC 3 cut(s) 991, 1423, 1503
LweI GCATC 1 cut(s) 1627
MaeI CTAG 7 cut(s) 96, 318, 663, 740, 839, 905, 2000
MaeIII GTNAC 4 cut(s) 137, 911, 1139, 1919
MbiI CCGCTC 1 cut(s) 1075
MfeI CAATTG 2 cut(s) 440, 1317
MflI RGATCY 5 cut(s) 201, 569, 1771, 1801, 2059
MhlI GDGCHC 2 cut(s) 996, 1500
MlyI GAGTC 3 cut(s) 586, 1055, 1141
MmeI TCCRAC 5 cut(s) 42, 427, 579, 1143, 1302
Mph1103I ATGCAT 1 cut(s) 1578
MroXI GAANNNNTTC 1 cut(s) 1527
MseI TTAA 8 cut(s) 225, 254, 597, 1323, 1332, 1557, 1827, 2086
MslI CAYNNNNRTG 2 cut(s) 621, 672
MspA1I CMGCKG 1 cut(s) 1908
MspI CCGG 5 cut(s) 87, 107, 263, 642, 1852
MunI CAATTG 2 cut(s) 440, 1317
Mva1269I GAATGC 1 cut(s) 1158
MwoI GCNNNNNNNGC 2 cut(s) 302, 1831
NlaIII CATG 7 cut(s) 467, 671, 1389, 1880, 1978, 2057, 2085
NlaIV GGNNCC 4 cut(s) 571, 880, 925, 1773
NmuCI GTSAC 2 cut(s) 911, 1919
NsiI ATGCAT 1 cut(s) 1578
NspI RCATGY 1 cut(s) 1978
PagI TCATGA 2 cut(s) 463, 667
PceI AGGCCT 1 cut(s) 1418
PciI ACATGT 1 cut(s) 1974
PctI GAATGC 1 cut(s) 1158
PdmI GAANNNNTTC 1 cut(s) 1527
PinAI ACCGGT 1 cut(s) 641
PkrI GCNGC 1 cut(s) 1074
PleI GAGTC 3 cut(s) 585, 1054, 1141
PpsI GAGTC 3 cut(s) 585, 1054, 1141
PpuMI RGGWCCY 1 cut(s) 1686
PscI ACATGT 1 cut(s) 1974
Psp124BI GAGCTC 1 cut(s) 996
Psp5II RGGWCCY 1 cut(s) 1686
PspEI GGTNACC 1 cut(s) 1919
PspN4I GGNNCC 4 cut(s) 571, 880, 925, 1773
PspPI GGNCC 3 cut(s) 304, 879, 1686
PspPPI RGGWCCY 1 cut(s) 1686
PstI CTGCAG 1 cut(s) 1209
PsuI RGATCY 5 cut(s) 201, 569, 1771, 1801, 2059
RsaI GTAC 5 cut(s) 105, 236, 456, 640, 853
RsaNI GTAC 5 cut(s) 104, 235, 455, 639, 852
RseI CAYNNNNRTG 2 cut(s) 621, 672
SacI GAGCTC 1 cut(s) 996
SaqAI TTAA 8 cut(s) 225, 254, 597, 1323, 1332, 1557, 1827, 2086
SatI GCNGC 1 cut(s) 1073
Sau96I GGNCC 3 cut(s) 304, 879, 1686
SchI GAGTC 3 cut(s) 586, 1055, 1141
SduI GDGCHC 2 cut(s) 996, 1500
SfaNI GCATC 1 cut(s) 1627
SfcI CTRYAG 1 cut(s) 1205
SinI GGWCC 2 cut(s) 879, 1686
SmiMI CAYNNNNRTG 2 cut(s) 621, 672
SmlI CTYRAG 4 cut(s) 733, 995, 1076, 1938
SmoI CTYRAG 4 cut(s) 733, 995, 1076, 1938
SpeI ACTAGT 2 cut(s) 662, 1999
SseBI AGGCCT 1 cut(s) 1418
SsiI CCGC 4 cut(s) 272, 1073, 1734, 1908
SspI AATATT 1 cut(s) 252
SspMI CTAG 7 cut(s) 96, 318, 663, 740, 839, 905, 2000
SstI GAGCTC 1 cut(s) 996
StuI AGGCCT 1 cut(s) 1418
StyI CCWWGG 1 cut(s) 1419
TaaI ACNGT 2 cut(s) 103, 1655
TatI WGTACW 1 cut(s) 454
TauI GCSGC 1 cut(s) 1075
Tru1I TTAA 8 cut(s) 225, 254, 597, 1323, 1332, 1557, 1827, 2086
Tru9I TTAA 8 cut(s) 225, 254, 597, 1323, 1332, 1557, 1827, 2086
TscAI CASTG 4 cut(s) 106, 537, 1406, 1654
TseFI GTSAC 2 cut(s) 911, 1919
Tsp45I GTSAC 2 cut(s) 911, 1919
TspGWI ACGGA 2 cut(s) 13, 1116
TspRI CASTG 4 cut(s) 106, 537, 1406, 1654
VpaK11BI GGWCC 2 cut(s) 879, 1686
XapI RAATTY 4 cut(s) 82, 176, 214, 227
XbaI TCTAGA 1 cut(s) 739
XceI RCATGY 1 cut(s) 1978
XmiI GTMKAC 1 cut(s) 1294
XmnI GAANNNNTTC 1 cut(s) 1527
XspI CTAG 7 cut(s) 96, 318, 663, 740, 839, 905, 2000
Zsp2I ATGCAT 1 cut(s) 1578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.