MD09G1050700.v1.1

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002986
Physical Location & Seq
Reverse (-)
3363794 .. 3367599
3806 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1050700.v1.1.491

Sequence Viewer

Length: 2292 bp
ATGGGTGCCTTAATCATTTCTTTGCTAAATACCCACTTTGATTGGGAGAATATGGGTGCTTCTGGTGGGGAGTTTTACTCAAAGATGGCTCTTTTTCCTAGACTTTTCACCCTCTCCGTCTCTTCCCTTTTGCTTGCTTTGTTAAGATTTTTCAGCAAGTTCTTCCTCAAAGTCAGTTTTGGTAATGGCGAGCGTTCTGGGATGAACAATTCTTGTCGTTCTCCTAACCCTCTTTCTGAAGAAACCAACCAAATGGTATTGAATAATAATCTTGTTGAGACAGAAGCTCTACCCGGAAAAGTGGATTGTTGTTCTGCTAGCCTTGTTTCTGAAGAAACCAATCAAATGGTATCGAATAATCCGGTTGAGCCAGAAGCTCTGCCGGAGAGAGCTGATTCCGAGATTTCGAAGAGCGACGGAGATTCGGAGCTGGGTGATGCTGATGGTGCGGAGGCCTCGAAGCCAAGTTTCGTGTTTAAATTCGAGTACCAGACTAGAGAAATATTAAGCAGAAGCAATAAAGAGTATGAGTTTCTGGCTGACAAGTTGAACCTATTCAAAGAAGAAGGACCAGAATTCACAAGCAGCAGCAGCAGCAGAAAATACGAATTCAAAGCCGAAGAGAATGTTAGTTGCTTCGTGGAAGAAGCTAAAGTTGCAACCTTTACTGTCGAAGAAGGAGGTGATCATGAAGAAATTGCTCATGAATTTTCAGGCGGGGAATGCGAGGAAGATGAAGAGTCTGATAAGAAATCGGCGATGGAAGATGCTCGTTCGGACGAAGAGCTAATGGGGAGCCTCGAAAGCGATTCTGATTGTGAGGAAGTTGATTCTCATGAAGTTAATTTCTTGTCAGAGAAGGACTTTGGCTCCCCAAGTTTGGGGTTTTTGTCAGCAGAAGATTTTGGGGAAAGGGATGAACTTCCAAATTTCGATATGGGGTACGAGTCTGATTGTTTCGAGGAAGAAGATGACGATATAAACGAACAAATTCGAGAACTCCAAGAGTTGCCCAGCAGCTCGAATCCGGTAGGGTTGAGTAGAAAGGACGAAAAGCCAATTGAGGGTTCCGAAAATGCTGAAAAACCCGATGTTCGTAATTCGGTAACCAATGATTCTGAGGATTCAAACACATTGGAAACATTGTGGGAACACCAGGAATTGATGGAACAGTTGAAAATGGAGCTCAAGAAAGTCAGAGCTACCGGTTTGCCTACCATTCTCGAAGAATCCGAGTCTCCGAATATGGATGATTTGAAACCATGGAAGATTGATGAAAAAGCCCATCAAGGTGATAGAATGGGAGAGCTTCAGACGTTCCACAGAATTTACAGAGAGCGTATGCGAAAATTCGACATTTTAAATTACCAGAAGTTATATGCAATAGGTTTCTTGCAGTCCAAGGATCCACTTCAGTCGTTTCCGAGCTCCTCCAAATCCTCAGCTCCGGCAATCCCATCCTTTCTCTCACAGAACTTTTGGAGATGCAAACCGAAAAACGATTCAGATTCCGACCCAATGGTGAAGTTTATTAGAGAATTGCATAGTGATTTAGAAGTGGTTTATGTGGGGCAGCTGTGCCTTTCTTGGGAGATCCTTCAATGGCAGTATGAGAAGGCCCTCAACCTATGGGATTCCGCTCCGTATGGAATGGGTTCGTACAATGTGGTTGCCGGCGAGTTCCAACAGTTTCAAGTTCACTTGGTTAGATTTGTAGAGAACGAATGTTTTCAAGGGCCTAGGGTTGAAAATTACGTCAAGAATCGATGCGTCATGCGCCATCTTCTTCAAGTTCCGGCGATTAGAGGGGACAATTTAAAGGAGAGGAAGAAAGCAAGAAGAAAGGGCAAAGACGGTGGTGCAATTACAAGTGACATTCTAGTAGAGATACTCGAAGAATCAATACGGACAATTTGGCGATTCATTCGAGCTGATAAATATGCTAATTGTACGCTTGCACATCGAAGGAGAAGACAAGAAGAAGAGCTTGAAGAATCGGATCTAAAGCTTTTCACGGAGATCCAAACAGATCTTCACAAGAAAAACATGAAGCTAAAAGAGAGACTGAGGAGTGGGAACTGCATCCTAAAGAGATTCAAAAAGCATGAGGATGAAGGCACAGATCACCTCTACTTCTTCTCCCAAGTTGACATGAAATTGGTAGCTAGGGTTTTGAACATGTCGAATATAACAACAGAGCAACTAGTGTGGTGTCACAGCAAGTTGAGCAAGATAAATTTTGTGAACCGAAAGCTTCGTGTAGATCCTTCCATCTTGCTATTTCCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

764

Amino Acids

87.47

Weight (kDa)

4.84

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 516 - 762 4.4e-94 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 5
AccBSI CCGCTC 1 cut(s) 1640
AciI CCGC 3 cut(s) 449, 717, 1638
AclWI GGATC 6 cut(s) 1400, 1413, 1588, 2007, 2014, 2258
AcsI RAATTY 9 cut(s) 479, 575, 608, 707, 928, 990, 1326, 1349, 2236
AcuI CTGAAG 4 cut(s) 258, 351, 1295, 1397
AfaI GTAC 4 cut(s) 488, 944, 1661, 1951
AfiI CCNNNNNNNGG 4 cut(s) 880, 881, 1064, 1588
AflIII ACRYGT 1 cut(s) 2178
AgeI ACCGGT 1 cut(s) 1205
AhlI ACTAGT 1 cut(s) 2203
AjnI CCWGG 1 cut(s) 1155
AjuI GAANNNNNNNTTGG 4 cut(s) 1051, 1083, 2016, 2048
Alw21I GWGCWC 2 cut(s) 1188, 1430
Alw26I GTCTC 4 cut(s) 124, 272, 1242, 2056
AlwI GGATC 6 cut(s) 1400, 1413, 1588, 2007, 2014, 2258
AoxI GGCC 3 cut(s) 453, 1617, 1736
ApeKI GCWGC 6 cut(s) 585, 588, 591, 594, 1017, 1573
ApoI RAATTY 9 cut(s) 479, 575, 608, 707, 928, 990, 1326, 1349, 2236
AsiGI ACCGGT 1 cut(s) 1205
Asp700I GAANNNNTTC 4 cut(s) 554, 990, 1654, 1728
AspA2I CCTAGG 1 cut(s) 1739
AspLEI GCGC 1 cut(s) 1779
AspS9I GGNCC 3 cut(s) 569, 1618, 1736
AsuC2I CCSGG 1 cut(s) 294
AsuHPI GGTGA 6 cut(s) 100, 446, 695, 1304, 1534, 2117
AsuII TTCGAA 1 cut(s) 407
AsuNHI GCTAGC 1 cut(s) 317
AvaII GGWCC 1 cut(s) 569
AvrII CCTAGG 1 cut(s) 1739
BamHI GGATCC 1 cut(s) 1405
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 2 cut(s) 1188, 1430
BarI GAAGNNNNNNTAC 2 cut(s) 1887, 1919
BbsI GAAGAC 1 cut(s) 1978
Bbv12I GWGCWC 2 cut(s) 1188, 1430
BbvCI CCTCAGC 1 cut(s) 1441
BbvI GCAGC 6 cut(s) 597, 600, 603, 606, 1029, 1585
BccI CCATC 8 cut(s) 79, 437, 754, 1159, 1293, 1465, 1788, 2279
BciT130I CCWGG 1 cut(s) 1157
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 4 cut(s) 124, 272, 1242, 2056
BcuI ACTAGT 1 cut(s) 2203
BfaI CTAG 8 cut(s) 99, 318, 495, 1740, 1880, 2166, 2204, 2290
BglII AGATCT 1 cut(s) 2029
BisI GCNGC 6 cut(s) 586, 589, 592, 595, 1018, 1574
BlnI CCTAGG 1 cut(s) 1739
BlsI GCNGC 6 cut(s) 587, 590, 593, 596, 1019, 1575
Bme1390I CCNGG 2 cut(s) 294, 1157
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 3 cut(s) 569, 1618, 1736
BmiI GGNNCC 5 cut(s) 7, 797, 871, 1069, 1407
BmrFI CCNGG 2 cut(s) 294, 1157
BmsI GCATC 5 cut(s) 427, 757, 1475, 1757, 2091
BmtI GCTAGC 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 1978
BplI GAGNNNNNCTC 2 cut(s) 62, 94
Bpu10I CCTNAGC 1 cut(s) 1441
Bpu14I TTCGAA 1 cut(s) 407
BpuEI CTTGAG 1 cut(s) 1172
BpuMI CCSGG 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 1765
BsaJI CCNNGG 3 cut(s) 1262, 1401, 1739
BsaWI WCCGGW 3 cut(s) 361, 1027, 1205
BsaXI ACNNNNNCTCC 4 cut(s) 854, 884, 2123, 2153
Bsc4I CCNNNNNNNGG 4 cut(s) 880, 881, 1064, 1588
Bse118I RCCGGY 2 cut(s) 1205, 1673
BseBI CCWGG 1 cut(s) 1157
BseCI ATCGAT 1 cut(s) 1765
BseDI CCNNGG 3 cut(s) 1262, 1401, 1739
BseGI GGATG 6 cut(s) 207, 922, 1255, 1457, 2082, 2116
BseLI CCNNNNNNNGG 4 cut(s) 880, 881, 1064, 1588
BseMII CTCAG 3 cut(s) 1110, 1455, 2057
BseRI GAGGAG 2 cut(s) 1420, 2083
BseXI GCAGC 6 cut(s) 597, 600, 603, 606, 1029, 1585
BseYI CCCAGC 2 cut(s) 430, 1013
BshFI GGCC 3 cut(s) 455, 1619, 1738
BshNI GGYRCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 1205
BshVI ATCGAT 1 cut(s) 1765
BsiHKAI GWGCWC 2 cut(s) 1188, 1430
BsiSI CCGG 8 cut(s) 294, 362, 383, 1028, 1206, 1448, 1674, 1796
BslFI GGGAC 1 cut(s) 1823
BslI CCNNNNNNNGG 4 cut(s) 880, 881, 1064, 1588
BsmAI GTCTC 4 cut(s) 124, 272, 1242, 2056
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 1 cut(s) 1823
BsmI GAATGC 1 cut(s) 728
BsnI GGCC 3 cut(s) 455, 1619, 1738
Bsp119I TTCGAA 1 cut(s) 407
Bsp1286I GDGCHC 2 cut(s) 1188, 1430
Bsp143I GATC 8 cut(s) 685, 1405, 1593, 1999, 2019, 2029, 2122, 2263
Bsp19I CCATGG 1 cut(s) 1262
BspACI CCGC 3 cut(s) 449, 717, 1638
BspANI GGCC 3 cut(s) 455, 1619, 1738
BspCNI CTCAG 3 cut(s) 1111, 1454, 2058
BspDI ATCGAT 1 cut(s) 1765
BspHI TCATGA 3 cut(s) 688, 703, 835
BspLI GGNNCC 5 cut(s) 7, 797, 871, 1069, 1407
BspOI GCTAGC 1 cut(s) 321
BspPI GGATC 6 cut(s) 1400, 1413, 1588, 2007, 2014, 2258
BspQI GCTCTTC 3 cut(s) 404, 777, 1977
BspT104I TTCGAA 1 cut(s) 407
BspT107I GGYRCC 1 cut(s) 5
BsrBI CCGCTC 1 cut(s) 1640
BsrFI RCCGGY 2 cut(s) 1205, 1673
BssAI RCCGGY 2 cut(s) 1205, 1673
BssECI CCNNGG 3 cut(s) 1262, 1401, 1739
BssMI GATC 8 cut(s) 685, 1405, 1593, 1999, 2019, 2029, 2122, 2263
BssT1I CCWWGG 3 cut(s) 1262, 1401, 1739
Bst2UI CCWGG 1 cut(s) 1157
Bst4CI ACNGT 4 cut(s) 670, 1173, 1689, 1856
Bst6I CTCTTC 6 cut(s) 127, 404, 615, 732, 777, 1977
BstBI TTCGAA 1 cut(s) 407
BstC8I GCNNGC 5 cut(s) 135, 191, 319, 1675, 1956
BstDEI CTNAG 3 cut(s) 1119, 1441, 2066
BstDSI CCRYGG 1 cut(s) 1262
BstEII GGTNACC 1 cut(s) 1105
BstF5I GGATG 6 cut(s) 207, 922, 1255, 1457, 2082, 2116
BstHHI GCGC 1 cut(s) 1779
BstKTI GATC 8 cut(s) 688, 1408, 1596, 2002, 2022, 2032, 2125, 2266
BstMAI GTCTC 4 cut(s) 124, 272, 1242, 2056
BstMBI GATC 8 cut(s) 685, 1405, 1593, 1999, 2019, 2029, 2122, 2263
BstMWI GCNNNNNNNGC 8 cut(s) 446, 591, 594, 656, 723, 804, 1776, 2226
BstNI CCWGG 1 cut(s) 1157
BstNSI RCATGY 1 cut(s) 2182
BstPI GGTNACC 1 cut(s) 1105
BstSCI CCNGG 2 cut(s) 292, 1155
BstV1I GCAGC 6 cut(s) 597, 600, 603, 606, 1029, 1585
BstV2I GAAGAC 1 cut(s) 1978
BstX2I RGATCY 6 cut(s) 1405, 1593, 1999, 2019, 2029, 2263
BstXI CCANNNNNNTGG 2 cut(s) 253, 346
BstYI RGATCY 6 cut(s) 1405, 1593, 1999, 2019, 2029, 2263
Bsu15I ATCGAT 1 cut(s) 1765
BsuRI GGCC 3 cut(s) 455, 1619, 1738
BsuTUI ATCGAT 1 cut(s) 1765
BtgI CCRYGG 1 cut(s) 1262
BtgZI GCGATG 1 cut(s) 773
BtsCI GGATG 6 cut(s) 207, 922, 1255, 1457, 2082, 2116
Cac8I GCNNGC 5 cut(s) 135, 191, 319, 1675, 1956
CciI TCATGA 3 cut(s) 688, 703, 835
CfoI GCGC 1 cut(s) 1779
Cfr10I RCCGGY 2 cut(s) 1205, 1673
Cfr13I GGNCC 3 cut(s) 569, 1618, 1736
ClaI ATCGAT 1 cut(s) 1765
CseI GACGC 1 cut(s) 1759
Csp6I GTAC 4 cut(s) 487, 943, 1660, 1950
CspAI ACCGGT 1 cut(s) 1205
CspCI CAANNNNNGTGG 6 cut(s) 23, 58, 1837, 1872, 2189, 2224
CviQI GTAC 4 cut(s) 487, 943, 1660, 1950
DdeI CTNAG 3 cut(s) 1119, 1441, 2066
DpnI GATC 8 cut(s) 687, 1407, 1595, 2001, 2021, 2031, 2124, 2265
DpnII GATC 8 cut(s) 685, 1405, 1593, 1999, 2019, 2029, 2122, 2263
DraI TTTAAA 3 cut(s) 478, 1362, 1818
Eam1104I CTCTTC 6 cut(s) 127, 404, 615, 732, 777, 1977
EarI CTCTTC 6 cut(s) 127, 404, 615, 732, 777, 1977
Ecl136II GAGCTC 2 cut(s) 1186, 1428
Eco130I CCWWGG 3 cut(s) 1262, 1401, 1739
Eco147I AGGCCT 1 cut(s) 455
Eco24I GRGCYC 2 cut(s) 1188, 1430
Eco47I GGWCC 1 cut(s) 569
Eco53kI GAGCTC 2 cut(s) 1186, 1428
Eco57I CTGAAG 4 cut(s) 258, 351, 1295, 1397
Eco91I GGTNACC 1 cut(s) 1105
EcoICRI GAGCTC 2 cut(s) 1186, 1428
EcoO109I RGGNCCY 2 cut(s) 1618, 1736
EcoO65I GGTNACC 1 cut(s) 1105
EcoRI GAATTC 2 cut(s) 575, 608
EcoRII CCWGG 1 cut(s) 1155
EcoT14I CCWWGG 3 cut(s) 1262, 1401, 1739
EcoT38I GRGCYC 2 cut(s) 1188, 1430
ErhI CCWWGG 3 cut(s) 1262, 1401, 1739
Esp3I CGTCTC 1 cut(s) 124
FalI AAGNNNNNCTT 2 cut(s) 1971, 2003
FaqI GGGAC 1 cut(s) 1823
FauI CCCGC 1 cut(s) 710
FbaI TGATCA 1 cut(s) 685
Fnu4HI GCNGC 6 cut(s) 586, 589, 592, 595, 1018, 1574
FokI GGATG 6 cut(s) 214, 929, 1262, 1444, 2069, 2123
FriOI GRGCYC 2 cut(s) 1188, 1430
Fsp4HI GCNGC 6 cut(s) 586, 589, 592, 595, 1018, 1574
FspBI CTAG 8 cut(s) 99, 318, 495, 1740, 1880, 2166, 2204, 2290
GlaI GCGC 1 cut(s) 1778
GluI GCNGC 6 cut(s) 586, 589, 592, 595, 1018, 1574
GsaI CCCAGC 2 cut(s) 434, 1017
HaeIII GGCC 3 cut(s) 455, 1619, 1738
HapII CCGG 8 cut(s) 294, 362, 383, 1028, 1206, 1448, 1674, 1796
HgaI GACGC 1 cut(s) 1759
HhaI GCGC 1 cut(s) 1779
Hin6I GCGC 1 cut(s) 1777
HinP1I GCGC 1 cut(s) 1777
HincII GTYRAC 1 cut(s) 2149
HindII GTYRAC 1 cut(s) 2149
HindIII AAGCTT 2 cut(s) 2006, 2252
HpaII CCGG 8 cut(s) 294, 362, 383, 1028, 1206, 1448, 1674, 1796
HphI GGTGA 6 cut(s) 100, 446, 695, 1304, 1534, 2117
Hpy166II GTNNAC 3 cut(s) 1699, 2149, 2245
Hpy188III TCNNGA 7 cut(s) 689, 704, 836, 995, 1189, 1223, 1759
Hpy8I GTNNAC 3 cut(s) 1699, 2149, 2245
Hpy99I CGWCG 1 cut(s) 419
HpyAV CCTTC 8 cut(s) 560, 671, 853, 1607, 1609, 1959, 2108, 2277
HpyCH4III ACNGT 4 cut(s) 670, 1173, 1689, 1856
HpyCH4IV ACGT 2 cut(s) 1316, 1755
HpyCH4V TGCA 8 cut(s) 659, 1382, 1396, 1488, 1543, 1862, 1958, 2082
HpyF10VI GCNNNNNNNGC 8 cut(s) 446, 591, 594, 656, 723, 804, 1776, 2226
HpyF3I CTNAG 3 cut(s) 1119, 1441, 2066
HpySE526I ACGT 2 cut(s) 1316, 1755
HspAI GCGC 1 cut(s) 1777
KroI GCCGGC 1 cut(s) 1673
KroNI GCCGGC 1 cut(s) 1675
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 8 cut(s) 685, 1405, 1593, 1999, 2019, 2029, 2122, 2263
LguI GCTCTTC 3 cut(s) 404, 777, 1977
LmnI GCTCC 7 cut(s) 427, 795, 875, 1183, 1433, 1450, 1645
Lsp1109I GCAGC 6 cut(s) 597, 600, 603, 606, 1029, 1585
LweI GCATC 5 cut(s) 427, 757, 1475, 1757, 2091
MaeI CTAG 8 cut(s) 99, 318, 495, 1740, 1880, 2166, 2204, 2290
MaeII ACGT 2 cut(s) 1316, 1755
MaeIII GTNAC 3 cut(s) 1105, 1871, 2213
MalI GATC 8 cut(s) 687, 1407, 1595, 2001, 2021, 2031, 2124, 2265
MbiI CCGCTC 1 cut(s) 1640
MboI GATC 8 cut(s) 685, 1405, 1593, 1999, 2019, 2029, 2122, 2263
MfeI CAATTG 1 cut(s) 1059
MflI RGATCY 6 cut(s) 1405, 1593, 1999, 2019, 2029, 2263
MhlI GDGCHC 2 cut(s) 1188, 1430
MlyI GAGTC 3 cut(s) 749, 956, 1244
MmeI TCCRAC 2 cut(s) 1536, 1708
MroNI GCCGGC 1 cut(s) 1673
MroXI GAANNNNTTC 4 cut(s) 554, 990, 1654, 1728
MseI TTAA 7 cut(s) 11, 143, 477, 506, 843, 1361, 1817
MslI CAYNNNNRTG 2 cut(s) 1290, 2109
MspA1I CMGCKG 1 cut(s) 1576
MspI CCGG 8 cut(s) 294, 362, 383, 1028, 1206, 1448, 1674, 1796
MspR9I CCNGG 2 cut(s) 294, 1157
MunI CAATTG 1 cut(s) 1059
Mva1269I GAATGC 1 cut(s) 728
MvaI CCWGG 1 cut(s) 1157
MwoI GCNNNNNNNGC 8 cut(s) 446, 591, 594, 656, 723, 804, 1776, 2226
NaeI GCCGGC 1 cut(s) 1675
NciI CCSGG 1 cut(s) 294
NcoI CCATGG 1 cut(s) 1262
NdeII GATC 8 cut(s) 685, 1405, 1593, 1999, 2019, 2029, 2122, 2263
NgoMIV GCCGGC 1 cut(s) 1673
NheI GCTAGC 1 cut(s) 317
NlaIV GGNNCC 5 cut(s) 7, 797, 871, 1069, 1407
NmuCI GTSAC 2 cut(s) 1871, 2213
NspI RCATGY 1 cut(s) 2182
NspV TTCGAA 1 cut(s) 407
PagI TCATGA 3 cut(s) 688, 703, 835
PceI AGGCCT 1 cut(s) 455
PciI ACATGT 1 cut(s) 2178
PciSI GCTCTTC 3 cut(s) 404, 777, 1977
PcsI WCGNNNNNNNCGW 2 cut(s) 981, 1230
PctI GAATGC 1 cut(s) 728
PdiI GCCGGC 1 cut(s) 1675
PdmI GAANNNNTTC 4 cut(s) 554, 990, 1654, 1728
PinAI ACCGGT 1 cut(s) 1205
PkrI GCNGC 6 cut(s) 587, 590, 593, 596, 1019, 1575
PleI GAGTC 3 cut(s) 748, 955, 1243
PpsI GAGTC 3 cut(s) 748, 955, 1243
PscI ACATGT 1 cut(s) 2178
Psp124BI GAGCTC 2 cut(s) 1188, 1430
Psp6I CCWGG 1 cut(s) 1155
PspEI GGTNACC 1 cut(s) 1105
PspFI CCCAGC 2 cut(s) 430, 1013
PspGI CCWGG 1 cut(s) 1155
PspN4I GGNNCC 5 cut(s) 7, 797, 871, 1069, 1407
PspPI GGNCC 3 cut(s) 569, 1618, 1736
PsuI RGATCY 6 cut(s) 1405, 1593, 1999, 2019, 2029, 2263
PvuII CAGCTG 1 cut(s) 1576
RsaI GTAC 4 cut(s) 488, 944, 1661, 1951
RsaNI GTAC 4 cut(s) 487, 943, 1660, 1950
RseI CAYNNNNRTG 2 cut(s) 1290, 2109
SacI GAGCTC 2 cut(s) 1188, 1430
SapI GCTCTTC 3 cut(s) 404, 777, 1977
SaqAI TTAA 7 cut(s) 11, 143, 477, 506, 843, 1361, 1817
SatI GCNGC 6 cut(s) 586, 589, 592, 595, 1018, 1574
Sau3AI GATC 8 cut(s) 685, 1405, 1593, 1999, 2019, 2029, 2122, 2263
Sau96I GGNCC 3 cut(s) 569, 1618, 1736
SchI GAGTC 3 cut(s) 749, 956, 1244
ScrFI CCNGG 2 cut(s) 294, 1157
SduI GDGCHC 2 cut(s) 1188, 1430
SfaNI GCATC 5 cut(s) 427, 757, 1475, 1757, 2091
SfuI TTCGAA 1 cut(s) 407
SinI GGWCC 1 cut(s) 569
SmiMI CAYNNNNRTG 2 cut(s) 1290, 2109
SmlI CTYRAG 1 cut(s) 1187
SmoI CTYRAG 1 cut(s) 1187
SpeI ACTAGT 1 cut(s) 2203
SseBI AGGCCT 1 cut(s) 455
SsiI CCGC 3 cut(s) 449, 717, 1638
SspI AATATT 1 cut(s) 504
SspMI CTAG 8 cut(s) 99, 318, 495, 1740, 1880, 2166, 2204, 2290
SstI GAGCTC 2 cut(s) 1188, 1430
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 2 cut(s) 292, 1155
StyI CCWWGG 3 cut(s) 1262, 1401, 1739
TaaI ACNGT 4 cut(s) 670, 1173, 1689, 1856
TaiI ACGT 2 cut(s) 1319, 1758
Tru1I TTAA 7 cut(s) 11, 143, 477, 506, 843, 1361, 1817
Tru9I TTAA 7 cut(s) 11, 143, 477, 506, 843, 1361, 1817
TseFI GTSAC 2 cut(s) 1871, 2213
TseI GCWGC 6 cut(s) 585, 588, 591, 594, 1017, 1573
Tsp45I GTSAC 2 cut(s) 1871, 2213
TspGWI ACGGA 5 cut(s) 106, 432, 1632, 1921, 2030
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 9 cut(s) 479, 575, 608, 707, 928, 990, 1326, 1349, 2236
XceI RCATGY 1 cut(s) 2182
XmaJI CCTAGG 1 cut(s) 1739
XmnI GAANNNNTTC 4 cut(s) 554, 990, 1654, 1728
XspI CTAG 8 cut(s) 99, 318, 495, 1740, 1880, 2166, 2204, 2290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.