Rh2DG627800

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002986
Physical Location & Seq
Forward (+)
85760820 .. 85765304
4485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG627800.1

Sequence Viewer

Length: 2229 bp
ATGCCCCAAAAAGTTGAGTCTGAGGTTTCAAAAAGCGATGATGATTCGGAGTACTATGATGTTGAAGATGAAAAAGAGACGCCGGAGCCAATTTTTGTGTTCAAATTTGAGTATCAGACTAAAGCCGTATTAGAAGCAATGGAGAAAGTGAATGCTTATGGTCATTCTTCTGTTGGAACCAACGAAGAGAGTGAGTTCCTACCCGAGAAATTTAGCGGCAGAAGGTACGAGTTTCAAGCCAAAAAGAATGTTAGTTTCTATGAGGAAGAAGCGGAAGTTGCAAGCTTTGTTGTCAGAGAAGCACCTGCTGATGACTCAAGTTGCGCTTCCATGGTGATTGAAAACGAGGGTGTTAAGCAATTGGTTACAGAAGAATGTGTTGAAGAAGAAGAAGAAGCAAAAGTTGCGAGCTTTGTTGTCGAAGAAGAATCCGCTGATAAATCAAATTTTGGTTCCATTGTGACCGGAAGTGAGAGTGTTAAGCAACTGGTCACAGAAGATTGTGTTCATGAAGACATTCAAAATCCTCAGGATTCGAAGAAGAAACAAGTCGGGGAAGATTCTCATTCGGATAAAGAGGAACAATTGAAGTCTCAAGAACATGACTTTGTGGATGAGAAAGTTGCTTCTCATGAAGATAAGTTTCCGCAAGAAAAGGACTTTGTTTTAGTAGAATCTGCTTCTGATTCTGATTCGGGTTCTCTTTGCTCAAGCCCTGAGCTTTCATTTATAGGTCAATTTAGTGGTGATGGATTTTTGTTAGAAACAGATTTTGGGGAAAGGGTTGAATTTGGTGCTTTGGAAGACATCGATTTGCAGAATTTGGATGTTGGGTATGAGCCTGATGGATTTGATGAAGAAGATGAAGATATAATGGAACAACTTAAAGAGCTAGAAGAGTCAACTAGGAATGATGAACATCACAACCCAAAAGAGTCTAAACCTGAAGGATTTACTGCCAAAGATGAAAAACCAAATGAGGGTTTAGAAAATTCTGAACAGGTCAGTGGTCAAGATATGGCAGCTAAAGATTCTGAGGATCCGAACACATTGGAGACACTGTGGGAACATCAAGAATTGATAGAACAGTTGAAAATGGAGCTCAAGAAAGTCCGGGTTTCTGGTCTGCCTACTATCCTTGAAGAATCCGAGTCTCCGAATATGGAGGATTTGAAGCCATGGAAGATTGATGAAAAATACCATCATGTGGACAGAATGAGTGTGCTTCACAAGTTCTACAAGACCTACAGAGAACGAATGCGAAAATTCGACATTTTGAGTTACCAGAAGTTATATGCAATAGGATTTTTGCAGTCCAAGGACACGTTCGAAACCTTTTCAAGCTGCAAATCCTCAACTCCAGCAATCAAATCCTTTCTTTTGCAGAACTTTGGGCTATGCAAACCGAAGAAAAATTCTAATTCTGATTCTGACCCAATGGTTAAGTTCATTAGAGAATTGCACAGTGAATTGGAAGTGGTTTATGTTGGACAGTTGTGCCTTTCTTGGGAATTCCTTCAATGGCAATATGAGAAGGCATTGGAGCTTTGGGAATATGACGAATATGGATTACGTTCGTATAATGAAGTTGCCGAGGAATTTCAACAATTTCAAGTGCTCATGCAAAGATTTGTGGAGAATGAAAGCTTTCAAGGGCCAAGGGTCGAAACCTATGTCAAAAATCGATGTGTTATGCGGAATCTTATGCAAATTCCAGTGATTAGGGAGGACAGTATGAAGGAAAAAAAGAAAGCAAAGAGAAAAGGCAAAGACAATGATGATGCAGTTGGAAGTGATATTCTAGTAGAGATATTGCAGGAATCAATAAGGACCATTTGGCGATTCATACGAGCTGATAAATCTGCTAATACAACGGATGCTTGTAGAAAAAGATTAAAGTTGGAAGTTGAAGACTCCGTGGATCCAAAGCTATTAGTGGAAGTTCAAGCAGATCTTCAAAAGAAAGAAAAGAAGCTTAAAGAGCTGTCCCGGAGTGAGAACTGCATACTAAAGAGATTCAAAAAGCACCATGAAGAAGAAGAGGAAGAAGAGAGCGCAGAACACCTCTACTTCTTTTGTCAAGTTGACATCAAATTAGTGTCGAGGGTTTTAAACATGTCAAGCATAACAACTGATCAACTAGTCTGGTGTCACAATAAGCTAAGCAGGATACATATTGTGAACCGAAAGATTCGTGTAGATCCTGCTTTCTTACTATTTCCATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

742

Amino Acids

86.02

Weight (kDa)

4.7

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 489 - 741 9.1e-94 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 313
Acc36I ACCTGC 1 cut(s) 313
AccB7I CCANNNNNTGG 1 cut(s) 1207
AciI CCGC 5 cut(s) 216, 272, 432, 647, 1696
AclWI GGATC 5 cut(s) 1034, 1047, 1916, 1929, 2195
AcuI CTGAAG 1 cut(s) 966
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 2 cut(s) 53, 227
AfiI CCNNNNNNNGG 3 cut(s) 980, 1207, 1507
AflIII ACRYGT 2 cut(s) 1323, 2115
AhlI ACTAGT 1 cut(s) 2140
AjuI GAANNNNNNNTTGG 4 cut(s) 756, 788, 1310, 1342
Alw21I GWGCWC 2 cut(s) 1104, 1620
Alw26I GTCTC 4 cut(s) 71, 597, 1049, 1158
AlwI GGATC 5 cut(s) 1034, 1047, 1916, 1929, 2195
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 1 cut(s) 1655
ApeKI GCWGC 2 cut(s) 1022, 1344
ArsI GACNNNNNNTTYG 2 cut(s) 1659, 1691
Asp700I GAANNNNTTC 3 cut(s) 516, 1515, 1647
AspLEI GCGC 2 cut(s) 326, 2057
AspS9I GGNCC 2 cut(s) 1655, 1830
AsuC2I CCSGG 2 cut(s) 1115, 1990
AsuHPI GGTGA 2 cut(s) 346, 758
AsuII TTCGAA 2 cut(s) 536, 1329
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 1 cut(s) 1830
AxyI CCTNAGG 1 cut(s) 528
BamHI GGATCC 2 cut(s) 1039, 1921
BanII GRGCYC 1 cut(s) 1104
BbsI GAAGAC 3 cut(s) 519, 810, 1917
Bbv12I GWGCWC 2 cut(s) 1104, 1620
BbvI GCAGC 2 cut(s) 1034, 1331
BccI CCATC 3 cut(s) 743, 839, 1209
BceAI ACGGC 1 cut(s) 110
BciVI GTATCC 1 cut(s) 2163
BclI TGATCA 1 cut(s) 2134
BcnI CCSGG 2 cut(s) 1115, 1990
BcoDI GTCTC 4 cut(s) 71, 597, 1049, 1158
BcuI ACTAGT 1 cut(s) 2140
BfaI CTAG 4 cut(s) 893, 906, 1802, 2141
BfmI CTRYAG 1 cut(s) 1246
BfuAI ACCTGC 1 cut(s) 313
BfuI GTATCC 1 cut(s) 2163
BglII AGATCT 1 cut(s) 1951
BisI GCNGC 3 cut(s) 217, 1023, 1345
BlpI GCTNAGC 1 cut(s) 2162
BlsI GCNGC 3 cut(s) 218, 1024, 1346
BmcAI AGTACT 1 cut(s) 53
Bme1390I CCNGG 2 cut(s) 1115, 1990
Bme18I GGWCC 1 cut(s) 1830
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 2 cut(s) 1655, 1830
BmiI GGNNCC 5 cut(s) 87, 178, 454, 1041, 1923
BmrFI CCNGG 2 cut(s) 1115, 1990
BmsI GCATC 2 cut(s) 1771, 1867
BpiI GAAGAC 3 cut(s) 519, 810, 1917
BpmI CTGGAG 1 cut(s) 1344
Bpu10I CCTNAGC 1 cut(s) 717
Bpu1102I GCTNAGC 1 cut(s) 2162
Bpu14I TTCGAA 2 cut(s) 536, 1329
BpuEI CTTGAG 4 cut(s) 301, 579, 694, 1088
BpuMI CCSGG 2 cut(s) 1115, 1990
Bsa29I ATCGAT 2 cut(s) 810, 1684
BsaBI GATNNNNATC 1 cut(s) 918
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 6 cut(s) 330, 1178, 1317, 1593, 1658, 1917
BsaWI WCCGGW 1 cut(s) 464
BsaXI ACNNNNNCTCC 2 cut(s) 1046, 1076
Bsc4I CCNNNNNNNGG 3 cut(s) 980, 1207, 1507
Bse1I ACTGG 2 cut(s) 492, 1715
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 1 cut(s) 144
Bse8I GATNNNNATC 1 cut(s) 918
BseCI ATCGAT 2 cut(s) 810, 1684
BseDI CCNNGG 6 cut(s) 330, 1178, 1317, 1593, 1658, 1917
BseGI GGATG 3 cut(s) 619, 832, 1882
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 3 cut(s) 980, 1207, 1507
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 4 cut(s) 12, 542, 708, 1026
BseNI ACTGG 2 cut(s) 492, 1715
BseXI GCAGC 2 cut(s) 1034, 1331
BshFI GGCC 1 cut(s) 1657
BshVI ATCGAT 2 cut(s) 810, 1684
BsiHKAI GWGCWC 2 cut(s) 1104, 1620
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 4 cut(s) 83, 465, 1114, 1990
BslFI GGGAC 1 cut(s) 1972
BslI CCNNNNNNNGG 3 cut(s) 980, 1207, 1507
BsmAI GTCTC 4 cut(s) 71, 597, 1049, 1158
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 1972
BsmI GAATGC 2 cut(s) 157, 1263
BsnI GGCC 1 cut(s) 1657
BsoBI CYCGRG 1 cut(s) 203
Bsp119I TTCGAA 2 cut(s) 536, 1329
Bsp1286I GDGCHC 2 cut(s) 1104, 1620
Bsp143I GATC 5 cut(s) 1039, 1921, 1951, 2134, 2200
Bsp1720I GCTNAGC 1 cut(s) 2162
Bsp19I CCATGG 2 cut(s) 330, 1178
BspACI CCGC 5 cut(s) 216, 272, 432, 647, 1696
BspANI GGCC 1 cut(s) 1657
BspCNI CTCAG 4 cut(s) 13, 541, 709, 1027
BspDI ATCGAT 2 cut(s) 810, 1684
BspHI TCATGA 2 cut(s) 508, 631
BspLI GGNNCC 5 cut(s) 87, 178, 454, 1041, 1923
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 5 cut(s) 1034, 1047, 1916, 1929, 2195
BspT104I TTCGAA 2 cut(s) 536, 1329
BsrDI GCAATG 1 cut(s) 144
BsrI ACTGG 2 cut(s) 492, 1715
BssECI CCNNGG 6 cut(s) 330, 1178, 1317, 1593, 1658, 1917
BssMI GATC 5 cut(s) 1039, 1921, 1951, 2134, 2200
BssNI GRCGYC 1 cut(s) 80
BssT1I CCWWGG 4 cut(s) 330, 1178, 1317, 1658
Bst4CI ACNGT 5 cut(s) 1062, 1089, 1466, 1494, 1733
Bst6I CTCTTC 4 cut(s) 180, 891, 2034, 2043
BstACI GRCGYC 1 cut(s) 80
BstAPI GCANNNNNTGC 1 cut(s) 404
BstBI TTCGAA 2 cut(s) 536, 1329
BstC8I GCNNGC 2 cut(s) 283, 409
BstDEI CTNAG 5 cut(s) 21, 528, 717, 1035, 2162
BstDSI CCRYGG 3 cut(s) 330, 1178, 1917
BstF5I GGATG 3 cut(s) 619, 832, 1882
BstHHI GCGC 2 cut(s) 326, 2057
BstKTI GATC 5 cut(s) 1042, 1924, 1954, 2137, 2203
BstMAI GTCTC 4 cut(s) 71, 597, 1049, 1158
BstMBI GATC 5 cut(s) 1039, 1921, 1951, 2134, 2200
BstMWI GCNNNNNNNGC 3 cut(s) 278, 404, 1981
BstNSI RCATGY 1 cut(s) 2119
BstSCI CCNGG 2 cut(s) 1113, 1988
BstSFI CTRYAG 1 cut(s) 1246
BstV1I GCAGC 2 cut(s) 1034, 1331
BstV2I GAAGAC 3 cut(s) 519, 810, 1917
BstX2I RGATCY 4 cut(s) 1039, 1921, 1951, 2200
BstYI RGATCY 4 cut(s) 1039, 1921, 1951, 2200
Bsu15I ATCGAT 2 cut(s) 810, 1684
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 1 cut(s) 2163
BsuRI GGCC 1 cut(s) 1657
BsuTUI ATCGAT 2 cut(s) 810, 1684
BtgI CCRYGG 3 cut(s) 330, 1178, 1917
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 3 cut(s) 619, 832, 1882
BtsIMutI CAGTG 4 cut(s) 1012, 1058, 1471, 1722
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 2 cut(s) 283, 409
CciI TCATGA 2 cut(s) 508, 631
CfoI GCGC 2 cut(s) 326, 2057
Cfr13I GGNCC 2 cut(s) 1655, 1830
ClaI ATCGAT 2 cut(s) 810, 1684
CseI GACGC 1 cut(s) 88
Csp6I GTAC 2 cut(s) 52, 226
CviQI GTAC 2 cut(s) 52, 226
DdeI CTNAG 5 cut(s) 21, 528, 717, 1035, 2162
DpnI GATC 5 cut(s) 1041, 1923, 1953, 2136, 2202
DpnII GATC 5 cut(s) 1039, 1921, 1951, 2134, 2200
DraI TTTAAA 1 cut(s) 2112
Eam1104I CTCTTC 4 cut(s) 180, 891, 2034, 2043
EarI CTCTTC 4 cut(s) 180, 891, 2034, 2043
Ecl136II GAGCTC 1 cut(s) 1102
Eco130I CCWWGG 4 cut(s) 330, 1178, 1317, 1658
Eco24I GRGCYC 1 cut(s) 1104
Eco47I GGWCC 1 cut(s) 1830
Eco53kI GAGCTC 1 cut(s) 1102
Eco57I CTGAAG 1 cut(s) 966
Eco81I CCTNAGG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 203
EcoICRI GAGCTC 1 cut(s) 1102
EcoRI GAATTC 1 cut(s) 1511
EcoT14I CCWWGG 4 cut(s) 330, 1178, 1317, 1658
EcoT38I GRGCYC 1 cut(s) 1104
ErhI CCWWGG 4 cut(s) 330, 1178, 1317, 1658
Esp3I CGTCTC 1 cut(s) 71
FalI AAGNNNNNCTT 4 cut(s) 310, 342, 1938, 1970
FaqI GGGAC 1 cut(s) 1972
FbaI TGATCA 1 cut(s) 2134
Fnu4HI GCNGC 3 cut(s) 217, 1023, 1345
FokI GGATG 3 cut(s) 626, 839, 1889
FriOI GRGCYC 1 cut(s) 1104
Fsp4HI GCNGC 3 cut(s) 217, 1023, 1345
FspBI CTAG 4 cut(s) 893, 906, 1802, 2141
GlaI GCGC 2 cut(s) 325, 2056
GluI GCNGC 3 cut(s) 217, 1023, 1345
GsuI CTGGAG 1 cut(s) 1344
HaeIII GGCC 1 cut(s) 1657
HapII CCGG 4 cut(s) 83, 465, 1114, 1990
HgaI GACGC 1 cut(s) 88
HhaI GCGC 2 cut(s) 326, 2057
Hin1I GRCGYC 1 cut(s) 80
Hin6I GCGC 2 cut(s) 324, 2055
HinP1I GCGC 2 cut(s) 324, 2055
HincII GTYRAC 2 cut(s) 903, 2086
HindII GTYRAC 2 cut(s) 903, 2086
HindIII AAGCTT 3 cut(s) 283, 1645, 1973
HpaII CCGG 4 cut(s) 83, 465, 1114, 1990
HphI GGTGA 2 cut(s) 346, 758
Hpy166II GTNNAC 4 cut(s) 903, 1210, 2086, 2182
Hpy188III TCNNGA 7 cut(s) 509, 530, 596, 632, 1013, 1073, 1105
Hpy8I GTNNAC 4 cut(s) 903, 1210, 2086, 2182
HpyAV CCTTC 5 cut(s) 216, 941, 1526, 1528, 1732
HpyCH4III ACNGT 5 cut(s) 1062, 1089, 1466, 1494, 1733
HpyCH4IV ACGT 2 cut(s) 1325, 1573
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 404, 1981
HpyF3I CTNAG 5 cut(s) 21, 528, 717, 1035, 2162
HpySE526I ACGT 2 cut(s) 1325, 1573
Hsp92I GRCGYC 1 cut(s) 80
HspAI GCGC 2 cut(s) 324, 2055
Ksp22I TGATCA 1 cut(s) 2134
Kzo9I GATC 5 cut(s) 1039, 1921, 1951, 2134, 2200
LmnI GCTCC 3 cut(s) 85, 1099, 1543
Lsp1109I GCAGC 2 cut(s) 1034, 1331
LweI GCATC 2 cut(s) 1771, 1867
MaeI CTAG 4 cut(s) 893, 906, 1802, 2141
MaeII ACGT 2 cut(s) 1325, 1573
MaeIII GTNAC 5 cut(s) 364, 460, 490, 1280, 2150
MalI GATC 5 cut(s) 1041, 1923, 1953, 2136, 2202
MboI GATC 5 cut(s) 1039, 1921, 1951, 2134, 2200
MfeI CAATTG 2 cut(s) 359, 584
MflI RGATCY 4 cut(s) 1039, 1921, 1951, 2200
MhlI GDGCHC 2 cut(s) 1104, 1620
MlyI GAGTC 6 cut(s) 26, 308, 908, 944, 1160, 1907
MmeI TCCRAC 4 cut(s) 154, 1468, 1768, 1881
MroXI GAANNNNTTC 3 cut(s) 516, 1515, 1647
MseI TTAA 7 cut(s) 354, 480, 885, 1443, 1895, 1977, 2111
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 4 cut(s) 83, 465, 1114, 1990
MspR9I CCNGG 2 cut(s) 1115, 1990
MunI CAATTG 2 cut(s) 359, 584
Mva1269I GAATGC 2 cut(s) 157, 1263
MwoI GCNNNNNNNGC 3 cut(s) 278, 404, 1981
NciI CCSGG 2 cut(s) 1115, 1990
NcoI CCATGG 2 cut(s) 330, 1178
NdeII GATC 5 cut(s) 1039, 1921, 1951, 2134, 2200
NlaIV GGNNCC 5 cut(s) 87, 178, 454, 1041, 1923
NmeAIII GCCGAG 1 cut(s) 1618
NmuCI GTSAC 3 cut(s) 460, 490, 2150
NspI RCATGY 1 cut(s) 2119
NspV TTCGAA 2 cut(s) 536, 1329
PagI TCATGA 2 cut(s) 508, 631
PaqCI CACCTGC 1 cut(s) 313
PciI ACATGT 1 cut(s) 2115
PctI GAATGC 2 cut(s) 157, 1263
PdmI GAANNNNTTC 3 cut(s) 516, 1515, 1647
PflMI CCANNNNNTGG 1 cut(s) 1207
PfoI TCCNGGA 1 cut(s) 1988
PkrI GCNGC 3 cut(s) 218, 1024, 1346
PleI GAGTC 6 cut(s) 25, 308, 907, 943, 1159, 1907
PpsI GAGTC 6 cut(s) 25, 308, 907, 943, 1159, 1907
PscI ACATGT 1 cut(s) 2115
Psp124BI GAGCTC 1 cut(s) 1104
PspN4I GGNNCC 5 cut(s) 87, 178, 454, 1041, 1923
PspPI GGNCC 2 cut(s) 1655, 1830
PsuI RGATCY 4 cut(s) 1039, 1921, 1951, 2200
RsaI GTAC 2 cut(s) 53, 227
RsaNI GTAC 2 cut(s) 52, 226
SacI GAGCTC 1 cut(s) 1104
SaqAI TTAA 7 cut(s) 354, 480, 885, 1443, 1895, 1977, 2111
SatI GCNGC 3 cut(s) 217, 1023, 1345
Sau3AI GATC 5 cut(s) 1039, 1921, 1951, 2134, 2200
Sau96I GGNCC 2 cut(s) 1655, 1830
ScaI AGTACT 1 cut(s) 53
SchI GAGTC 6 cut(s) 26, 308, 908, 944, 1160, 1907
ScrFI CCNGG 2 cut(s) 1115, 1990
SduI GDGCHC 2 cut(s) 1104, 1620
SfaNI GCATC 2 cut(s) 1771, 1867
SfcI CTRYAG 1 cut(s) 1246
SfuI TTCGAA 2 cut(s) 536, 1329
SinI GGWCC 1 cut(s) 1830
SmlI CTYRAG 4 cut(s) 316, 594, 709, 1103
SmoI CTYRAG 4 cut(s) 316, 594, 709, 1103
SpeI ACTAGT 1 cut(s) 2140
SsiI CCGC 5 cut(s) 216, 272, 432, 647, 1696
SspMI CTAG 4 cut(s) 893, 906, 1802, 2141
SstI GAGCTC 1 cut(s) 1104
StyD4I CCNGG 2 cut(s) 1113, 1988
StyI CCWWGG 4 cut(s) 330, 1178, 1317, 1658
TaaI ACNGT 5 cut(s) 1062, 1089, 1466, 1494, 1733
TaiI ACGT 2 cut(s) 1328, 1576
TaqI TCGA 8 cut(s) 420, 536, 810, 1269, 1329, 1665, 1684, 2102
TatI WGTACW 1 cut(s) 51
TauI GCSGC 1 cut(s) 219
Tru1I TTAA 7 cut(s) 354, 480, 885, 1443, 1895, 1977, 2111
Tru9I TTAA 7 cut(s) 354, 480, 885, 1443, 1895, 1977, 2111
TscAI CASTG 4 cut(s) 1012, 1065, 1471, 1722
TseFI GTSAC 3 cut(s) 460, 490, 2150
TseI GCWGC 2 cut(s) 1022, 1344
Tsp45I GTSAC 3 cut(s) 460, 490, 2150
TspGWI ACGGA 2 cut(s) 1889, 1906
TspRI CASTG 4 cut(s) 1012, 1065, 1471, 1722
Van91I CCANNNNNTGG 1 cut(s) 1207
VpaK11BI GGWCC 1 cut(s) 1830
XceI RCATGY 1 cut(s) 2119
XmnI GAANNNNTTC 3 cut(s) 516, 1515, 1647
XspI CTAG 4 cut(s) 893, 906, 1802, 2141
ZrmI AGTACT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.