Rroxscaffold_6G00426990

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002986
Physical Location & Seq
Reverse (-)
47595460 .. 47604806
9347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00426990.1

Sequence Viewer

Length: 2082 bp
ATGATTGCTCAGTTTTCCGACATCTTTGTGTTCATATTCTTCAGCACACTCTTTGTCGAAGAAGAGAATTCTCCGATGAGGAATTCCGGTTGCTCTAGCACTGTACAGGAAAGAGCTGAATCTGATTCTGATTTCATTTGTTCGAGTAAAGATGATGATGATGATGATTCAGAATTCTTTGATGTTGGTGATATAGAGATCTCAGAGCCAAATTTCGTGTTTAAATTTGAGTACCAGACTAGGGAAATATTAAAAACACCGGAAAAAGCGGATGTAATTGGTGATTATGTAGCTTTTCAGGCCCAAAAGAATACTAGTAGTTGCTTAATTGAAGAAGCAGAAAATGGGTCCGCTCAGAAATCAGATTGTGGTTCTATCGAAGCAACTGATGTTGACGAAGGAAAAAGCGCTCAGGGTTTTACAGAGGGTTTTGACCAATTGGTTAGAGAAGTACAAGTTCATGAGGAAAAAGGTGATCAGGGTTTTAGAGATGGTGCATTTGAGGAAGAGGAGGTAGAGAAGAAATCAGTGATTGAAGAAGATTCTGTTTCGGACGACGAAGAAGGATCCGAGAGTCAGGAAGAAGTAGATGTTAATAATGAAGAAATGTCTCATTCAGATGAGGAAAGTGATAGTACCGGTGATGAGAAACTGGCTACTAGTCATGAAGATGAGTTGGTTTCGGAAGAGAACTTTGTTAGTTCGGAATCTGATTCTGATTTGATTTGCTCAAGTCTAGATGATGATGAAGTAGGTGCTTTGGAAGACATTGGATTGCACGAGCCTGATGGTTTTGATGAAGAAGATGATGATATAATGGAACAAGTTAGAGAACTAGAAGAGTTGTGTACGACTGATGATCAAAACTCGAAAGGGTCCGAACCTAAAGGAGAAGAAAAGCTAGTGGGTGATTATAATGGTTCCGAGGATTCAGACACATTGGACGTTTTGTGGGAACATCAAGAGTTGATAGACCAGTTGAAACTGGAGCTCAAGAAAGTCAGAGCTACAACTGGTCTGCCTACAATTTTTGAAGAATCCGAGTCTCTGGATATGGATGACTTGAAGCCGCTCAAGATTGATGAAAAATACCAACACGGAGATAGAATGAGGGTGCTTCACAAGTTCTACAAGAGTTACAGGACTCGAATGCGCAAGTTGGATAATTTGAATTACCAGAAATTATATGCAATAGGTGTTCTGCAGTCTAAGGATCCACTTAGATCGTTTTCGAGCTACAAATCCTCAACTCCAGCAATCAAATCCCTTCTTTCCAACTGGAGGCTATGTAGACCCAAAATAGATTGTGATCCAATTGTTAAGTTCATTAAGGATGTGCATAGTGAATTGGAATTGGTCTATGTAGGGCAGTTGTGCCTTTCATGGGAGTTCCTTCAATGGCAATATGAGAAGGCCTTGGAGCTATTGGAATCTGCCCCTAATCAAATACATTCATACAATGAAGTTGCAGAAGAATTGCAACATTTTCAAGTGCTCCTGCAAAGATTTATAGAGGATGAACATTTTCAAGGGGCAAGGGTCGAAAACTATGTTAAAAATCGACGTGCTATGCATAAGCTTCTACAAGTTCCAGTAATCAGAGAGGACAAATCGGAGGAAGGAAAGAAAGAGGATGCAATTACCACTGACAGTCTTGTCGAGATGTTGAGAGAATCGATAAGGACCATTTGGCGATTCATTCGAGCTGATAAAAATGCCAACATAACCACCGCTTGTCGAAAAAGGTCACTTGATGGAGATTGCTTGGATCCAGAGCTTTTAGTGGAAGTTCAAGCAGATCTTCGGAAGAAAGAGAGGAAGCTTAAAGAGCTATTGAGGAGTGAGAACCGGATCATAAAGAGATTCCAAAAGCATGAAGAAGGAGAAGAAGAGGAAGACAACGGAGATCATGGTCACCATAACTTCTTTTCTCAAGTGGACATCAGATTAGTTTCAAGGGTTTTGAACATGTCAACTGTAACACAAGAGCAACTAGTGTGGTGTCAAAGTAAGTTGAGCGAGATACACTTTGTGAAGCGCAAGATTCATGTAGATCCCTCTTTCTTGTTGTTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

693

Amino Acids

79.86

Weight (kDa)

4.49

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 448 - 692 6e-87 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 915
AasI GACNNNNNNGTC 1 cut(s) 1655
Acc16I TGCGCA 1 cut(s) 1154
AccBSI CCGCTC 2 cut(s) 353, 1072
AccI GTMKAC 1 cut(s) 1291
AciI CCGC 4 cut(s) 269, 351, 1070, 1731
AclWI GGATC 9 cut(s) 561, 574, 1208, 1221, 1304, 1763, 1776, 1859, 2048
AcsI RAATTY 5 cut(s) 67, 82, 173, 211, 224
AcuI CTGAAG 1 cut(s) 25
AdeI CACNNNGTG 1 cut(s) 2032
AfaI GTAC 5 cut(s) 105, 233, 453, 637, 850
AfeI AGCGCT 1 cut(s) 409
AfiI CCNNNNNNNGG 3 cut(s) 241, 1281, 1384
AflIII ACRYGT 1 cut(s) 1968
AgeI ACCGGT 1 cut(s) 638
AhlI ACTAGT 3 cut(s) 314, 659, 1993
AjiI CACGTC 1 cut(s) 1565
Alw21I GWGCWC 2 cut(s) 993, 1497
Alw26I GTCTC 2 cut(s) 615, 1050
AlwI GGATC 9 cut(s) 561, 574, 1208, 1221, 1304, 1763, 1776, 1859, 2048
Aor51HI AGCGCT 1 cut(s) 409
AoxI GGCC 2 cut(s) 300, 1413
ApoI RAATTY 5 cut(s) 67, 82, 173, 211, 224
AsiGI ACCGGT 1 cut(s) 638
Asp700I GAANNNNTTC 1 cut(s) 1524
AspLEI GCGC 3 cut(s) 410, 1155, 2040
AspS9I GGNCC 4 cut(s) 301, 348, 876, 1683
AsuHPI GGTGA 6 cut(s) 200, 293, 485, 653, 920, 1907
AvaII GGWCC 3 cut(s) 348, 876, 1683
BamHI GGATCC 3 cut(s) 566, 1213, 1768
BanII GRGCYC 1 cut(s) 993
BauI CACGAG 1 cut(s) 779
BbsI GAAGAC 2 cut(s) 771, 1902
Bbv12I GWGCWC 2 cut(s) 993, 1497
BccI CCATC 3 cut(s) 485, 782, 1748
BclI TGATCA 2 cut(s) 475, 859
BcoDI GTCTC 2 cut(s) 615, 1050
BcuI ACTAGT 3 cut(s) 314, 659, 1993
BfaI CTAG 8 cut(s) 96, 240, 315, 660, 737, 836, 902, 1994
BfmI CTRYAG 1 cut(s) 1202
BfoI RGCGCY 1 cut(s) 411
BglII AGATCT 2 cut(s) 198, 1798
BisI GCNGC 1 cut(s) 1070
BlsI GCNGC 1 cut(s) 1071
Bme18I GGWCC 3 cut(s) 348, 876, 1683
BmgBI CACGTC 1 cut(s) 1565
BmgT120I GGNCC 4 cut(s) 301, 348, 876, 1683
BmiI GGNNCC 6 cut(s) 349, 568, 877, 922, 1215, 1770
BmsI GCATC 1 cut(s) 1624
BpiI GAAGAC 2 cut(s) 771, 1902
BpmI CTGGAG 3 cut(s) 1007, 1236, 1300
Bpu10I CCTNAGC 1 cut(s) 411
BpuEI CTTGAG 4 cut(s) 715, 977, 1058, 1917
Bsa29I ATCGAT 1 cut(s) 1676
BsaBI GATNNNNATC 1 cut(s) 1308
BsaJI CCNNGG 2 cut(s) 924, 1416
BsaWI WCCGGW 4 cut(s) 86, 259, 638, 1848
BsaXI ACNNNNNCTCC 2 cut(s) 1273, 1303
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 1281, 1384
Bse118I RCCGGY 1 cut(s) 638
Bse1I ACTGG 6 cut(s) 657, 976, 990, 1018, 1283, 1592
Bse8I GATNNNNATC 1 cut(s) 1308
BseCI ATCGAT 1 cut(s) 1676
BseDI CCNNGG 2 cut(s) 924, 1416
BseGI GGATG 5 cut(s) 277, 1063, 1339, 1522, 1639
BseJI GATNNNNATC 1 cut(s) 1308
BseLI CCNNNNNNNGG 3 cut(s) 241, 1281, 1384
BseMII CTCAG 4 cut(s) 23, 216, 368, 425
BseNI ACTGG 6 cut(s) 657, 976, 990, 1018, 1283, 1592
BseRI GAGGAG 2 cut(s) 524, 1852
BshFI GGCC 2 cut(s) 302, 1415
BshTI ACCGGT 1 cut(s) 638
BshVI ATCGAT 1 cut(s) 1676
BsiHKAI GWGCWC 2 cut(s) 993, 1497
BsiSI CCGG 4 cut(s) 87, 260, 639, 1849
BslI CCNNNNNNNGG 3 cut(s) 241, 1281, 1384
BsmAI GTCTC 2 cut(s) 615, 1050
BsmI GAATGC 1 cut(s) 1155
BsnI GGCC 2 cut(s) 302, 1415
Bsp1286I GDGCHC 2 cut(s) 993, 1497
Bsp1407I TGTACA 1 cut(s) 103
BspACI CCGC 4 cut(s) 269, 351, 1070, 1731
BspANI GGCC 2 cut(s) 302, 1415
BspCNI CTCAG 4 cut(s) 22, 215, 367, 424
BspDI ATCGAT 1 cut(s) 1676
BspHI TCATGA 2 cut(s) 460, 664
BspLI GGNNCC 6 cut(s) 349, 568, 877, 922, 1215, 1770
BspMAI CTGCAG 1 cut(s) 1206
BspPI GGATC 9 cut(s) 561, 574, 1208, 1221, 1304, 1763, 1776, 1859, 2048
BsrBI CCGCTC 2 cut(s) 353, 1072
BsrFI RCCGGY 1 cut(s) 638
BsrGI TGTACA 1 cut(s) 103
BsrI ACTGG 6 cut(s) 657, 976, 990, 1018, 1283, 1592
BssAI RCCGGY 1 cut(s) 638
BssECI CCNNGG 2 cut(s) 924, 1416
BssSI CACGAG 1 cut(s) 779
BssT1I CCWWGG 1 cut(s) 1416
Bst2BI CACGAG 1 cut(s) 779
Bst4CI ACNGT 3 cut(s) 103, 1652, 1978
Bst6I CTCTTC 5 cut(s) 57, 501, 681, 834, 1884
BstAUI TGTACA 1 cut(s) 103
BstDEI CTNAG 6 cut(s) 9, 202, 354, 411, 1209, 1220
BstEII GGTNACC 1 cut(s) 1913
BstF5I GGATG 5 cut(s) 277, 1063, 1339, 1522, 1639
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 3 cut(s) 410, 1155, 2040
BstMAI GTCTC 2 cut(s) 615, 1050
BstMWI GCNNNNNNNGC 2 cut(s) 299, 1828
BstNSI RCATGY 1 cut(s) 1972
BstPI GGTNACC 1 cut(s) 1913
BstSFI CTRYAG 1 cut(s) 1202
BstV2I GAAGAC 2 cut(s) 771, 1902
BstX2I RGATCY 6 cut(s) 198, 566, 1213, 1768, 1798, 2053
BstYI RGATCY 6 cut(s) 198, 566, 1213, 1768, 1798, 2053
Bsu15I ATCGAT 1 cut(s) 1676
BsuRI GGCC 2 cut(s) 302, 1415
BsuTUI ATCGAT 1 cut(s) 1676
BtrI CACGTC 1 cut(s) 1565
BtsCI GGATG 5 cut(s) 277, 1063, 1339, 1522, 1639
BtsIMutI CAGTG 3 cut(s) 99, 534, 1644
CciI TCATGA 2 cut(s) 460, 664
CfoI GCGC 3 cut(s) 410, 1155, 2040
Cfr10I RCCGGY 1 cut(s) 638
Cfr13I GGNCC 4 cut(s) 301, 348, 876, 1683
ClaI ATCGAT 1 cut(s) 1676
Csp6I GTAC 5 cut(s) 104, 232, 452, 636, 849
CspAI ACCGGT 1 cut(s) 638
CspCI CAANNNNNGTGG 2 cut(s) 1979, 2014
CviAII CATG 8 cut(s) 461, 665, 1383, 1874, 1910, 1969, 2048, 2076
CviQI GTAC 5 cut(s) 104, 232, 452, 636, 849
DdeI CTNAG 6 cut(s) 9, 202, 354, 411, 1209, 1220
DraI TTTAAA 1 cut(s) 223
DraIII CACNNNGTG 1 cut(s) 2032
DrdI GACNNNNNNGTC 1 cut(s) 1655
DseDI GACNNNNNNGTC 1 cut(s) 1655
Eam1104I CTCTTC 5 cut(s) 57, 501, 681, 834, 1884
EarI CTCTTC 5 cut(s) 57, 501, 681, 834, 1884
Ecl136II GAGCTC 1 cut(s) 991
Eco130I CCWWGG 1 cut(s) 1416
Eco147I AGGCCT 1 cut(s) 1415
Eco24I GRGCYC 1 cut(s) 993
Eco47I GGWCC 3 cut(s) 348, 876, 1683
Eco47III AGCGCT 1 cut(s) 409
Eco53kI GAGCTC 1 cut(s) 991
Eco57I CTGAAG 1 cut(s) 25
Eco91I GGTNACC 1 cut(s) 1913
EcoICRI GAGCTC 1 cut(s) 991
EcoO65I GGTNACC 1 cut(s) 1913
EcoRI GAATTC 3 cut(s) 67, 82, 173
EcoT14I CCWWGG 1 cut(s) 1416
EcoT22I ATGCAT 1 cut(s) 1575
EcoT38I GRGCYC 1 cut(s) 993
ErhI CCWWGG 1 cut(s) 1416
FaeI CATG 8 cut(s) 464, 668, 1386, 1877, 1913, 1972, 2051, 2079
FalI AAGNNNNNCTT 2 cut(s) 1785, 1817
FatI CATG 8 cut(s) 460, 664, 1382, 1873, 1909, 1968, 2047, 2075
FbaI TGATCA 2 cut(s) 475, 859
FblI GTMKAC 1 cut(s) 1291
Fnu4HI GCNGC 1 cut(s) 1070
FokI GGATG 5 cut(s) 284, 1070, 1346, 1529, 1646
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 1 cut(s) 1070
FspBI CTAG 8 cut(s) 96, 240, 315, 660, 737, 836, 902, 1994
FspI TGCGCA 1 cut(s) 1154
GlaI GCGC 3 cut(s) 409, 1154, 2039
GluI GCNGC 1 cut(s) 1070
GsuI CTGGAG 3 cut(s) 1007, 1236, 1300
HaeII RGCGCY 1 cut(s) 411
HaeIII GGCC 2 cut(s) 302, 1415
HapII CCGG 4 cut(s) 87, 260, 639, 1849
HhaI GCGC 3 cut(s) 410, 1155, 2040
Hin1II CATG 8 cut(s) 464, 668, 1386, 1877, 1913, 1972, 2051, 2079
Hin6I GCGC 3 cut(s) 408, 1153, 2038
HinP1I GCGC 3 cut(s) 408, 1153, 2038
HincII GTYRAC 2 cut(s) 394, 1974
HindII GTYRAC 2 cut(s) 394, 1974
HindIII AAGCTT 2 cut(s) 1577, 1820
HpaII CCGG 4 cut(s) 87, 260, 639, 1849
HphI GGTGA 6 cut(s) 200, 293, 485, 653, 920, 1907
Hpy166II GTNNAC 5 cut(s) 394, 849, 1292, 1939, 1974
Hpy8I GTNNAC 5 cut(s) 394, 849, 1292, 1939, 1974
Hpy99I CGWCG 2 cut(s) 560, 1566
HpyAV CCTTC 7 cut(s) 392, 557, 1277, 1403, 1405, 1613, 1874
HpyCH4III ACNGT 3 cut(s) 103, 1652, 1978
HpyCH4IV ACGT 2 cut(s) 945, 1564
HpyF10VI GCNNNNNNNGC 2 cut(s) 299, 1828
HpyF3I CTNAG 6 cut(s) 9, 202, 354, 411, 1209, 1220
HpySE526I ACGT 2 cut(s) 945, 1564
Hsp92II CATG 8 cut(s) 464, 668, 1386, 1877, 1913, 1972, 2051, 2079
HspAI GCGC 3 cut(s) 408, 1153, 2038
Ksp22I TGATCA 2 cut(s) 475, 859
LmnI GCTCC 3 cut(s) 988, 1420, 1500
LweI GCATC 1 cut(s) 1624
MaeI CTAG 8 cut(s) 96, 240, 315, 660, 737, 836, 902, 1994
MaeII ACGT 2 cut(s) 945, 1564
MaeIII GTNAC 4 cut(s) 1136, 1746, 1913, 1978
MbiI CCGCTC 2 cut(s) 353, 1072
MfeI CAATTG 2 cut(s) 437, 1314
MflI RGATCY 6 cut(s) 198, 566, 1213, 1768, 1798, 2053
MhlI GDGCHC 2 cut(s) 993, 1497
MlyI GAGTC 3 cut(s) 583, 1052, 1138
MmeI TCCRAC 3 cut(s) 42, 1140, 1299
Mph1103I ATGCAT 1 cut(s) 1575
MroXI GAANNNNTTC 1 cut(s) 1524
MseI TTAA 9 cut(s) 222, 251, 326, 594, 1320, 1329, 1554, 1824, 2080
MslI CAYNNNNRTG 3 cut(s) 26, 618, 669
MspI CCGG 4 cut(s) 87, 260, 639, 1849
MunI CAATTG 2 cut(s) 437, 1314
Mva1269I GAATGC 1 cut(s) 1155
MwoI GCNNNNNNNGC 2 cut(s) 299, 1828
NlaIII CATG 8 cut(s) 464, 668, 1386, 1877, 1913, 1972, 2051, 2079
NlaIV GGNNCC 6 cut(s) 349, 568, 877, 922, 1215, 1770
NmuCI GTSAC 2 cut(s) 1746, 1913
NsbI TGCGCA 1 cut(s) 1154
NsiI ATGCAT 1 cut(s) 1575
NspI RCATGY 1 cut(s) 1972
PagI TCATGA 2 cut(s) 460, 664
PceI AGGCCT 1 cut(s) 1415
PciI ACATGT 1 cut(s) 1968
PctI GAATGC 1 cut(s) 1155
PdmI GAANNNNTTC 1 cut(s) 1524
PinAI ACCGGT 1 cut(s) 638
PkrI GCNGC 1 cut(s) 1071
PleI GAGTC 3 cut(s) 582, 1051, 1138
PpsI GAGTC 3 cut(s) 582, 1051, 1138
PscI ACATGT 1 cut(s) 1968
PsiI TTATAA 1 cut(s) 915
Psp124BI GAGCTC 1 cut(s) 993
PspEI GGTNACC 1 cut(s) 1913
PspN4I GGNNCC 6 cut(s) 349, 568, 877, 922, 1215, 1770
PspPI GGNCC 4 cut(s) 301, 348, 876, 1683
PstI CTGCAG 1 cut(s) 1206
PsuI RGATCY 6 cut(s) 198, 566, 1213, 1768, 1798, 2053
RsaI GTAC 5 cut(s) 105, 233, 453, 637, 850
RsaNI GTAC 5 cut(s) 104, 232, 452, 636, 849
RseI CAYNNNNRTG 3 cut(s) 26, 618, 669
SacI GAGCTC 1 cut(s) 993
SaqAI TTAA 9 cut(s) 222, 251, 326, 594, 1320, 1329, 1554, 1824, 2080
SatI GCNGC 1 cut(s) 1070
Sau96I GGNCC 4 cut(s) 301, 348, 876, 1683
SchI GAGTC 3 cut(s) 583, 1052, 1138
SduI GDGCHC 2 cut(s) 993, 1497
SfaNI GCATC 1 cut(s) 1624
SfcI CTRYAG 1 cut(s) 1202
SinI GGWCC 3 cut(s) 348, 876, 1683
SmiMI CAYNNNNRTG 3 cut(s) 26, 618, 669
SmlI CTYRAG 4 cut(s) 730, 992, 1073, 1932
SmoI CTYRAG 4 cut(s) 730, 992, 1073, 1932
SpeI ACTAGT 3 cut(s) 314, 659, 1993
SseBI AGGCCT 1 cut(s) 1415
SsiI CCGC 4 cut(s) 269, 351, 1070, 1731
SspI AATATT 1 cut(s) 249
SspMI CTAG 8 cut(s) 96, 240, 315, 660, 737, 836, 902, 1994
SstI GAGCTC 1 cut(s) 993
StuI AGGCCT 1 cut(s) 1415
StyI CCWWGG 1 cut(s) 1416
TaaI ACNGT 3 cut(s) 103, 1652, 1978
TaiI ACGT 2 cut(s) 948, 1567
TatI WGTACW 2 cut(s) 103, 451
TauI GCSGC 1 cut(s) 1072
Tru1I TTAA 9 cut(s) 222, 251, 326, 594, 1320, 1329, 1554, 1824, 2080
Tru9I TTAA 9 cut(s) 222, 251, 326, 594, 1320, 1329, 1554, 1824, 2080
TscAI CASTG 3 cut(s) 106, 534, 1651
TseFI GTSAC 2 cut(s) 1746, 1913
Tsp45I GTSAC 2 cut(s) 1746, 1913
TspGWI ACGGA 2 cut(s) 1113, 1917
TspRI CASTG 3 cut(s) 106, 534, 1651
VpaK11BI GGWCC 3 cut(s) 348, 876, 1683
XapI RAATTY 5 cut(s) 67, 82, 173, 211, 224
XbaI TCTAGA 1 cut(s) 736
XceI RCATGY 1 cut(s) 1972
XmiI GTMKAC 1 cut(s) 1291
XmnI GAANNNNTTC 1 cut(s) 1524
XspI CTAG 8 cut(s) 96, 240, 315, 660, 737, 836, 902, 1994
Zsp2I ATGCAT 1 cut(s) 1575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.