MD01G1191200.v1.1

Leucine-rich repeats, outliers

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002986
Physical Location & Seq
Reverse (-)
28953010 .. 28954674
1665 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1191200.v1.1.491

Sequence Viewer

Length: 1665 bp
ATGGCTTCTGCTGCCGCTTGGGTTTTTCAAACAAAGATGGCACTTTTTATCTCTTCACTTTTGCTTACTTTGTTCAGATTCTTCAGCAAAATCTTCCTTGTGAGTTTTCGTCACGACAAGAGTTCTCCGTTGAACAATTCCTGTTGTTCTAGCCCTCCTCCTTCGGGAGAAATCAATGGGATTTCGAAGATTGATGGTGATGATTCGAAGTTTGATGATGTTGGTGATGGAGATGCGTCGAAACCGAATTTCGTGTTCAAGTTTGAGTTCCAGACTAAAGAATTATTGAGTAGAATCAATGAAGGGAATAATGTTAATGGTGATTCGGCTCTCTTGGAAGAGTACGAGTTCCTAGCTGAGAAGTTTAACCAGATGAAAGCTGCAAAGAATGTTAGTTGCTTCATGGACGAAGCTAAAGCCGCAAGCTTTAATGTTGAAGAAGCTCCTCAGGATTTTGGACATGGTGAATGCGAGAAAGACGAGTCCCGTGAGATGAAATTGGTCACGGGAGAGGAGCCTCGTTCGGACGGAGAGAAATTGGGGAGTCATGAAAACGATTCGGCTTCAGATTCTGATTACGATTATGTTGGGTACGCAACTGATTGTTTTGAGGACGAAGGAGAAGATATGAAGAAAGAAATTCGAGAAACCAAAGAGCTGCAGAAGAATGATCATCACAATCCTGAAGAAAAGCCAACGGAGGGTTTAGATTTAGAAGATTCTGAAAAATCCAATTGTCATAACCATAACCTGACAGCCAGAGATTATGTGGATTCATACACATTGGAAGCTTTGTGGGAGCACCAAGAATTGATAGAACAGTTAAAAATGGAGTTCAAGAGAGTCAGAGCTGATGCTCTGCCTACTATTCCCGAAGATTACGAGTTCTTGAATTTGGATGAATTGAAGCCAAGGAAGGTTGATGAAATACTCCATGGTAGAATCGGAGAGTTTGACGAATTCTACAAGAGCTACAGCGTAGGTATGCGAAATTTCGATATCTTGAATAACCAGAAGTTGAATGCACTACGTTTATTGCAGTCCAAGAAACCACCTAAGCCGTTTTCGAGCAGCAAATCCTTTTGCTCACAGAACCTTTTGAGATGCAAATCAAATAAAGATTCTGATTTTTTTGACCCAATTTCGGTGAAGTTTAGAGAATTGCATAGTGAGTTAGAAGTGGTGTATGTGGGGCAGTTGTGCCTTTCGTGGGAGTTCCTTAAATGGCAGTATGGTAAGGCCTTGGAGCTATGGAAGTCTCCATATAAAACCGCTACGTATAATGAAGTCGCGGGCGAATTTCAGAAGTTTAAAGAGTACTTGCAAAGATTTGTGGAGGGTGAATGTTCTGAAGAACGGACAAGGGTTGAAAATTATGTCAGTAATCGATACAAGATGTCTCTACTTCTTCAAGTTCCAGTGGTTCGAGGGGACAGTTTAAAAGACAGAAGGAAAACAAGAAGAAAAGGTAGAGATGATGGAGAAATAATTTCGAGTGACGATCTAGTAGAGAAACTGGAAGAATCAATTACGACAATGTGGCAATTCATTCGAGCTGATAAATACGCTAGCAGTTCGATTAATGCAAGTCAAAAGAGAAGACGAGAACACCTTCAAGAAACGGATTTGAATCTTTTGGCTGAGGTCCAAACTGATCTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8

Protein Analysis

555

Amino Acids

63.98

Weight (kDa)

4.99

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 390 - 553 5.1e-47 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1292
AciI CCGC 4 cut(s) 15, 420, 1272, 1292
AcsI RAATTY 6 cut(s) 247, 639, 892, 959, 991, 1298
AcuI CTGAAG 4 cut(s) 67, 549, 705, 1371
AfaI GTAC 3 cut(s) 344, 593, 1319
AfiI CCNNNNNNNGG 4 cut(s) 164, 701, 1144, 1210
AjuI GAANNNNNNNTTGG 1 cut(s) 1641
Alw21I GWGCWC 1 cut(s) 804
Alw26I GTCTC 2 cut(s) 1263, 1404
AlwNI CAGNNNCTG 1 cut(s) 572
AoxI GGCC 1 cut(s) 1239
ApeKI GCWGC 4 cut(s) 11, 380, 658, 1071
ApoI RAATTY 6 cut(s) 247, 639, 892, 959, 991, 1298
AseI ATTAAT 1 cut(s) 1581
Asp700I GAANNNNTTC 1 cut(s) 1611
AspS9I GGNCC 1 cut(s) 1645
AsuHPI GGTGA 6 cut(s) 209, 236, 332, 476, 1159, 1352
AsuII TTCGAA 2 cut(s) 185, 206
AsuNHI GCTAGC 1 cut(s) 1568
AvaII GGWCC 1 cut(s) 1645
AxyI CCTNAGG 1 cut(s) 447
BbsI GAAGAC 1 cut(s) 1606
Bbv12I GWGCWC 1 cut(s) 804
BbvCI CCTCAGC 1 cut(s) 1641
BbvI GCAGC 3 cut(s) 367, 645, 1083
BccI CCATC 4 cut(s) 31, 188, 221, 1472
BceAI ACGGC 1 cut(s) 1045
BcgI CGANNNNNNTGC 2 cut(s) 1532, 1566
BclI TGATCA 1 cut(s) 670
BcoDI GTCTC 2 cut(s) 1263, 1404
BfaI CTAG 4 cut(s) 150, 353, 1505, 1569
BfmI CTRYAG 2 cut(s) 659, 973
BisI GCNGC 6 cut(s) 12, 15, 381, 420, 659, 1072
BlsI GCNGC 6 cut(s) 13, 16, 382, 421, 660, 1073
BmcAI AGTACT 1 cut(s) 1319
Bme18I GGWCC 1 cut(s) 1645
BmgT120I GGNCC 1 cut(s) 1645
BmiI GGNNCC 1 cut(s) 516
BmsI GCATC 3 cut(s) 223, 844, 1094
BmtI GCTAGC 1 cut(s) 1572
BpiI GAAGAC 1 cut(s) 1606
Bpu10I CCTNAGC 2 cut(s) 1056, 1641
Bpu14I TTCGAA 2 cut(s) 185, 206
Bsa29I ATCGAT 1 cut(s) 1387
BsaAI YACGTR 1 cut(s) 1278
BsaBI GATNNNNATC 2 cut(s) 1108, 1629
BsaJI CCNNGG 3 cut(s) 911, 934, 1242
Bsc4I CCNNNNNNNGG 4 cut(s) 164, 701, 1144, 1210
Bse1I ACTGG 2 cut(s) 1418, 1521
Bse21I CCTNAGG 1 cut(s) 447
Bse8I GATNNNNATC 2 cut(s) 1108, 1629
BseCI ATCGAT 1 cut(s) 1387
BseDI CCNNGG 3 cut(s) 911, 934, 1242
BseGI GGATG 1 cut(s) 904
BseJI GATNNNNATC 2 cut(s) 1108, 1629
BseLI CCNNNNNNNGG 4 cut(s) 164, 701, 1144, 1210
BseMII CTCAG 3 cut(s) 348, 461, 1632
BseNI ACTGG 2 cut(s) 1418, 1521
BseRI GAGGAG 3 cut(s) 147, 435, 527
BseXI GCAGC 3 cut(s) 367, 645, 1083
Bsh1236I CGCG 1 cut(s) 1292
BshFI GGCC 1 cut(s) 1241
BshVI ATCGAT 1 cut(s) 1387
BsiHKAI GWGCWC 1 cut(s) 804
BslFI GGGAC 2 cut(s) 469, 1445
BslI CCNNNNNNNGG 4 cut(s) 164, 701, 1144, 1210
BsmAI GTCTC 2 cut(s) 1263, 1404
BsmFI GGGAC 2 cut(s) 469, 1445
BsmI GAATGC 2 cut(s) 473, 1027
BsnI GGCC 1 cut(s) 1241
Bsp119I TTCGAA 2 cut(s) 185, 206
Bsp1286I GDGCHC 1 cut(s) 804
Bsp143I GATC 3 cut(s) 670, 1501, 1654
Bsp19I CCATGG 1 cut(s) 934
BspACI CCGC 4 cut(s) 15, 420, 1272, 1292
BspANI GGCC 1 cut(s) 1241
BspCNI CTCAG 3 cut(s) 349, 460, 1633
BspDI ATCGAT 1 cut(s) 1387
BspFNI CGCG 1 cut(s) 1292
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 1 cut(s) 516
BspMAI CTGCAG 1 cut(s) 663
BspOI GCTAGC 1 cut(s) 1572
BspT104I TTCGAA 2 cut(s) 185, 206
BsrI ACTGG 2 cut(s) 1418, 1521
BssECI CCNNGG 3 cut(s) 911, 934, 1242
BssMI GATC 3 cut(s) 670, 1501, 1654
BssT1I CCWWGG 3 cut(s) 911, 934, 1242
Bst4CI ACNGT 2 cut(s) 822, 1436
Bst6I CTCTTC 2 cut(s) 58, 333
BstBAI YACGTR 1 cut(s) 1278
BstBI TTCGAA 2 cut(s) 185, 206
BstC8I GCNNGC 3 cut(s) 424, 1294, 1570
BstDEI CTNAG 4 cut(s) 357, 447, 1056, 1641
BstDSI CCRYGG 1 cut(s) 934
BstF5I GGATG 1 cut(s) 904
BstFNI CGCG 1 cut(s) 1292
BstKTI GATC 3 cut(s) 673, 1504, 1657
BstMAI GTCTC 2 cut(s) 1263, 1404
BstMBI GATC 3 cut(s) 670, 1501, 1654
BstMWI GCNNNNNNNGC 2 cut(s) 11, 419
BstSFI CTRYAG 2 cut(s) 659, 973
BstSNI TACGTA 1 cut(s) 1278
BstUI CGCG 1 cut(s) 1292
BstV1I GCAGC 3 cut(s) 367, 645, 1083
BstV2I GAAGAC 1 cut(s) 1606
Bsu15I ATCGAT 1 cut(s) 1387
Bsu36I CCTNAGG 1 cut(s) 447
BsuRI GGCC 1 cut(s) 1241
BsuTUI ATCGAT 1 cut(s) 1387
BtgI CCRYGG 1 cut(s) 934
BtsCI GGATG 1 cut(s) 904
BtsIMutI CAGTG 1 cut(s) 1425
Cac8I GCNNGC 3 cut(s) 424, 1294, 1570
CaiI CAGNNNCTG 1 cut(s) 572
CciI TCATGA 1 cut(s) 547
Cfr13I GGNCC 1 cut(s) 1645
ClaI ATCGAT 1 cut(s) 1387
CseI GACGC 1 cut(s) 225
Csp6I GTAC 3 cut(s) 343, 592, 1318
CviAII CATG 4 cut(s) 403, 461, 548, 935
CviQI GTAC 3 cut(s) 343, 592, 1318
DdeI CTNAG 4 cut(s) 357, 447, 1056, 1641
DpnI GATC 3 cut(s) 672, 1503, 1656
DpnII GATC 3 cut(s) 670, 1501, 1654
DraI TTTAAA 2 cut(s) 1312, 1440
Eam1104I CTCTTC 2 cut(s) 58, 333
EarI CTCTTC 2 cut(s) 58, 333
Eco105I TACGTA 1 cut(s) 1278
Eco130I CCWWGG 3 cut(s) 911, 934, 1242
Eco147I AGGCCT 1 cut(s) 1241
Eco32I GATATC 1 cut(s) 1000
Eco47I GGWCC 1 cut(s) 1645
Eco57I CTGAAG 4 cut(s) 67, 549, 705, 1371
Eco81I CCTNAGG 1 cut(s) 447
EcoRI GAATTC 1 cut(s) 959
EcoRV GATATC 1 cut(s) 1000
EcoT14I CCWWGG 3 cut(s) 911, 934, 1242
ErhI CCWWGG 3 cut(s) 911, 934, 1242
FaeI CATG 4 cut(s) 406, 464, 551, 938
FaqI GGGAC 2 cut(s) 469, 1445
FatI CATG 4 cut(s) 402, 460, 547, 934
FauI CCCGC 1 cut(s) 1285
FbaI TGATCA 1 cut(s) 670
Fnu4HI GCNGC 6 cut(s) 12, 15, 381, 420, 659, 1072
FokI GGATG 1 cut(s) 911
Fsp4HI GCNGC 6 cut(s) 12, 15, 381, 420, 659, 1072
FspBI CTAG 4 cut(s) 150, 353, 1505, 1569
GluI GCNGC 6 cut(s) 12, 15, 381, 420, 659, 1072
HaeIII GGCC 1 cut(s) 1241
HgaI GACGC 1 cut(s) 225
Hin1II CATG 4 cut(s) 406, 464, 551, 938
HindIII AAGCTT 2 cut(s) 424, 789
HphI GGTGA 6 cut(s) 209, 236, 332, 476, 1159, 1352
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 6 cut(s) 171, 296, 611, 910, 1443, 1622
HpyCH4III ACNGT 2 cut(s) 822, 1436
HpyCH4IV ACGT 2 cut(s) 1030, 1277
HpyCH4V TGCA 8 cut(s) 383, 661, 1025, 1039, 1107, 1165, 1324, 1586
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 419
HpyF3I CTNAG 4 cut(s) 357, 447, 1056, 1641
HpySE526I ACGT 2 cut(s) 1030, 1277
Hsp92II CATG 4 cut(s) 406, 464, 551, 938
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 3 cut(s) 670, 1501, 1654
LmnI GCTCC 4 cut(s) 448, 514, 799, 1246
Lsp1109I GCAGC 3 cut(s) 367, 645, 1083
LweI GCATC 3 cut(s) 223, 844, 1094
MaeI CTAG 4 cut(s) 150, 353, 1505, 1569
MaeII ACGT 2 cut(s) 1030, 1277
MaeIII GTNAC 3 cut(s) 110, 502, 1496
MalI GATC 3 cut(s) 672, 1503, 1656
MboI GATC 3 cut(s) 670, 1501, 1654
MfeI CAATTG 1 cut(s) 733
MhlI GDGCHC 1 cut(s) 804
MlyI GAGTC 3 cut(s) 491, 553, 852
MroXI GAANNNNTTC 1 cut(s) 1611
MseI TTAA 8 cut(s) 315, 366, 429, 824, 1221, 1311, 1439, 1581
MunI CAATTG 1 cut(s) 733
Mva1269I GAATGC 2 cut(s) 473, 1027
MvnI CGCG 1 cut(s) 1292
MwoI GCNNNNNNNGC 2 cut(s) 11, 419
NcoI CCATGG 1 cut(s) 934
NdeII GATC 3 cut(s) 670, 1501, 1654
NheI GCTAGC 1 cut(s) 1568
NlaIII CATG 4 cut(s) 406, 464, 551, 938
NlaIV GGNNCC 1 cut(s) 516
NmuCI GTSAC 3 cut(s) 110, 502, 1496
NspV TTCGAA 2 cut(s) 185, 206
PagI TCATGA 1 cut(s) 547
PceI AGGCCT 1 cut(s) 1241
PctI GAATGC 2 cut(s) 473, 1027
PdmI GAANNNNTTC 1 cut(s) 1611
PkrI GCNGC 6 cut(s) 13, 16, 382, 421, 660, 1073
PleI GAGTC 3 cut(s) 490, 552, 851
PpsI GAGTC 3 cut(s) 490, 552, 851
Ppu21I YACGTR 1 cut(s) 1278
PshBI ATTAAT 1 cut(s) 1581
PspN4I GGNNCC 1 cut(s) 516
PspPI GGNCC 1 cut(s) 1645
PstI CTGCAG 1 cut(s) 663
PstNI CAGNNNCTG 1 cut(s) 572
RsaI GTAC 3 cut(s) 344, 593, 1319
RsaNI GTAC 3 cut(s) 343, 592, 1318
SaqAI TTAA 8 cut(s) 315, 366, 429, 824, 1221, 1311, 1439, 1581
SatI GCNGC 6 cut(s) 12, 15, 381, 420, 659, 1072
Sau3AI GATC 3 cut(s) 670, 1501, 1654
Sau96I GGNCC 1 cut(s) 1645
ScaI AGTACT 1 cut(s) 1319
SchI GAGTC 3 cut(s) 491, 553, 852
SduI GDGCHC 1 cut(s) 804
SfaNI GCATC 3 cut(s) 223, 844, 1094
SfcI CTRYAG 2 cut(s) 659, 973
SfuI TTCGAA 2 cut(s) 185, 206
SinI GGWCC 1 cut(s) 1645
SnaBI TACGTA 1 cut(s) 1278
SseBI AGGCCT 1 cut(s) 1241
SsiI CCGC 4 cut(s) 15, 420, 1272, 1292
SspMI CTAG 4 cut(s) 150, 353, 1505, 1569
StuI AGGCCT 1 cut(s) 1241
StyI CCWWGG 3 cut(s) 911, 934, 1242
TaaI ACNGT 2 cut(s) 822, 1436
TaiI ACGT 2 cut(s) 1033, 1280
TatI WGTACW 1 cut(s) 1317
TauI GCSGC 2 cut(s) 17, 422
Tru1I TTAA 8 cut(s) 315, 366, 429, 824, 1221, 1311, 1439, 1581
Tru9I TTAA 8 cut(s) 315, 366, 429, 824, 1221, 1311, 1439, 1581
TscAI CASTG 1 cut(s) 1425
TseFI GTSAC 3 cut(s) 110, 502, 1496
TseI GCWGC 4 cut(s) 11, 380, 658, 1071
Tsp45I GTSAC 3 cut(s) 110, 502, 1496
TspGWI ACGGA 5 cut(s) 117, 543, 713, 1372, 1637
TspRI CASTG 1 cut(s) 1425
VpaK11BI GGWCC 1 cut(s) 1645
VspI ATTAAT 1 cut(s) 1581
XapI RAATTY 6 cut(s) 247, 639, 892, 959, 991, 1298
XcmI CCANNNNNNNNNTGG 1 cut(s) 766
XmnI GAANNNNTTC 1 cut(s) 1611
XspI CTAG 4 cut(s) 150, 353, 1505, 1569
ZrmI AGTACT 1 cut(s) 1319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.