AT5G56510

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001707
Physical Location & Seq
Forward (+)
22880687 .. 22884087
3401 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G56510.1

Sequence Viewer

Length: 1791 bp
ATGGATCAGAGAAGAGGAAATGAGCTTGATGAATTTGAGAAGCTTCTAGGAGAGATTCCAAAAGTTACTTCAGGAAACGACTATAACCATTTCCCTATATGTTTGAGCTCAAGCAGATCACAATCCATCAAGAAGGTTGATCAATATCTTCCTGATGACCGTGCCTTTACCACTTCATTTTCCGAGGCTAACTTACACTTTGGAATCCCAAATCACACTCCAGAGTCTCCCCATCCTTTGTTCATTAACCCTTCTTACCACTCACCAAGTAACTCACCTTGTGTATATGACAAGTTTGATTCAAGAAAACTCGATCCGGTAATGTTCAGGAAGCTGCAACAAGTTGGATACCTTCCAAACTTGTCTTCAGGGATCTCACCTGCTCAGCGGCAGCATTACCTGCCACATTCGCAGCCTCTGTCTCACTATCAATCACCTATGACTTGGAGGGATATCGAAGAAGAAAATTTTCAGAGGCTTAAACTTCAAGAAGAACAGTATTTGTCTATTAACCCTCATTTCCTCCATCTTCAGAGCATGGATACTGTTCCAAGACAGGACCATTTCGATTATCGCCGAGCTGAACAGTCTAACAGAAACTTGTTTTGGAATGGAGAAGATGGTAATGAAAGTGTGAGGAAAATGTGCTATCCGGAGAAGATTTTAATGAGATCACAGATGGATTTGAACACTGCTAAAGTCATAAAGTATGGTGCTGGAGATGAGTCACAAAATGGAAGACTTTGGTTGCAGAATCAACTCAATGAAGATCTCACAATGAGTCTCAATAATCTGTCATTGCAGCCTCAAAAGTATAACTCTATTGCAGAGGCAAGAGGGAAGATATACTACTTGGCCAAGGATCAGCACGGTTGTCGCTTCTTGCAGAGAATATTTTCTGAGAAAGATGGGAATGATATAGAGATGATCTTTAATGAGATCATTGACTATATCAGTGAGCTAATGATGGATCCTTTTGGGAACTATTTGGTTCAAAAGCTGCTAGAAGTATGCAATGAGGATCAGAGGATGCAGATTGTTCATTCCATAACTAGAAAACCAGGACTGCTTATCAAAATCTCTTGTGATATGCACGGGACTAGAGCTGTTCAAAAGATAGTTGAAACGGCTAAGAGAGAGGAGGAGATTTCAATCATCATTTCTGCTTTGAAGCATGGCATTGTGCATTTGATAAAGAATGTAAACGGTAATCACGTTGTACAACGATGTTTGCAGTATCTGTTACCTTACTGCGGAAAGTTCCTTTTCGAAGCTGCGATTACTCATTGTGTTGAGCTTGCAACTGATAGACATGGATGTTGTGTACTTCAAAAATGTCTTGGATATTCAGAAGGCGAACAAAAGCAACATTTAGTCTCTGAAATTGCGTCCAATGCTCTACTCCTCTCTCAAGATCCTTTTGGGAACTATGTACTTCAATATGTCTTTGAGCTTCAACTTCAATGGGCAACCTTTGAAATCCTGGAGCAATTAGAAGGAAACTACACCGAGTTATCGATGCAGAAATGTAGCAGCAATGTAGTTGAAAAGTGTCTGAAACTAGCTGATGACAAACACCGAGCTCGCATCATCAGAGAATTGATTAACTATGGTCGTCTTGATCAAGTGATGTTGGATCCTTATGGAAATTATGTCATTCAAGCAGCTCTTAAACAATCCAAGGGGAATGTTCATGCTCTTTTGGTTGATGCCATTAAACTGAATATCTCATCTCTTCGTACCAATCCTTACGGTAAAAAAGTCCTCTCCGCACTTAGCTCGAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

596

Amino Acids

68.67

Weight (kDa)

6.63

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF_NOP9 PF22493 260 - 412 8.6e-07 NOP9-like PUF repeat domain
PUF PF00806 282 - 315 1.3e-10 Pumilio-family RNA binding repeat
PUF PF00806 318 - 350 5.7e-11 Pumilio-family RNA binding repeat
PUF PF00806 429 - 462 1.8e-15 Pumilio-family RNA binding repeat
PUF PF00806 502 - 535 2e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 388
Acc36I ACCTGC 2 cut(s) 388, 408
AccIII TCCGGA 1 cut(s) 652
AciI CCGC 3 cut(s) 388, 1254, 1770
AcoI YGGCCR 1 cut(s) 855
AcsI RAATTY 2 cut(s) 32, 466
AcuI CTGAAG 3 cut(s) 54, 351, 515
AdeI CACNNNGTG 1 cut(s) 281
AfaI GTAC 4 cut(s) 1221, 1326, 1434, 1741
AfiI CCNNNNNNNGG 1 cut(s) 1253
AjnI CCWGG 2 cut(s) 1060, 1482
AjuI GAANNNNNNNTTGG 6 cut(s) 52, 84, 349, 381, 589, 621
Alw21I GWGCWC 2 cut(s) 110, 1585
Alw26I GTCTC 4 cut(s) 231, 426, 788, 1381
AlwNI CAGNNNCTG 2 cut(s) 418, 1240
Aor13HI TCCGGA 1 cut(s) 652
AoxI GGCC 1 cut(s) 855
ApeKI GCWGC 8 cut(s) 334, 391, 412, 802, 1000, 1274, 1533, 1664
ApoI RAATTY 2 cut(s) 32, 466
Asp700I GAANNNNTTC 2 cut(s) 468, 895
AspS9I GGNCC 1 cut(s) 559
AsuHPI GGTGA 4 cut(s) 255, 267, 369, 426
AsuII TTCGAA 1 cut(s) 1269
AvaII GGWCC 1 cut(s) 559
BalI TGGCCA 1 cut(s) 857
BamHI GGATCC 2 cut(s) 970, 1636
BanII GRGCYC 2 cut(s) 110, 1585
BarI GAAGNNNNNNTAC 2 cut(s) 833, 865
BbsI GAAGAC 2 cut(s) 357, 745
Bbv12I GWGCWC 2 cut(s) 110, 1585
BbvI GCAGC 8 cut(s) 321, 403, 424, 814, 987, 1261, 1545, 1676
BccI CCATC 7 cut(s) 134, 240, 534, 614, 673, 902, 961
BceAI ACGGC 1 cut(s) 1143
BcgI CGANNNNNNTGC 3 cut(s) 857, 891, 1761
BciT130I CCWGG 2 cut(s) 1062, 1484
BciVI GTATCC 2 cut(s) 341, 535
BclI TGATCA 2 cut(s) 139, 1621
BcoDI GTCTC 4 cut(s) 231, 426, 788, 1381
BfaI CTAG 5 cut(s) 47, 1004, 1053, 1101, 1562
BfuAI ACCTGC 2 cut(s) 388, 408
BfuI GTATCC 2 cut(s) 341, 535
BglII AGATCT 1 cut(s) 769
BisI GCNGC 9 cut(s) 335, 389, 392, 413, 803, 1001, 1275, 1534, 1665
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 9 cut(s) 336, 390, 393, 414, 804, 1002, 1276, 1535, 1666
Bme1390I CCNGG 2 cut(s) 1062, 1484
Bme18I GGWCC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 2 cut(s) 972, 1638
BmrFI CCNGG 2 cut(s) 1062, 1484
BmsI GCATC 4 cut(s) 1020, 1509, 1596, 1699
BpiI GAAGAC 2 cut(s) 357, 745
BpmI CTGGAG 3 cut(s) 204, 738, 1505
Bpu1102I GCTNAGC 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 1269
BpuEI CTTGAG 2 cut(s) 94, 1395
Bsa29I ATCGAT 1 cut(s) 1517
BsaBI GATNNNNATC 3 cut(s) 121, 144, 1151
BsaJI CCNNGG 3 cut(s) 183, 858, 1680
BsaWI WCCGGW 2 cut(s) 316, 652
Bsc4I CCNNNNNNNGG 1 cut(s) 1253
Bse3DI GCAATG 3 cut(s) 797, 1021, 1543
Bse8I GATNNNNATC 3 cut(s) 121, 144, 1151
BseAI TCCGGA 1 cut(s) 652
BseBI CCWGG 2 cut(s) 1062, 1484
BseCI ATCGAT 1 cut(s) 1517
BseDI CCNNGG 3 cut(s) 183, 858, 1680
BseGI GGATG 3 cut(s) 232, 1035, 1322
BseJI GATNNNNATC 3 cut(s) 121, 144, 1151
BseLI CCNNNNNNNGG 1 cut(s) 1253
BseMI GCAATG 3 cut(s) 797, 1021, 1543
BseMII CTCAG 2 cut(s) 398, 891
BseRI GAGGAG 3 cut(s) 1154, 1157, 1394
BseXI GCAGC 8 cut(s) 321, 403, 424, 814, 987, 1261, 1545, 1676
BshFI GGCC 1 cut(s) 857
BshVI ATCGAT 1 cut(s) 1517
BsiHKAI GWGCWC 2 cut(s) 110, 1585
BsiSI CCGG 2 cut(s) 317, 653
BslFI GGGAC 1 cut(s) 1111
BslI CCNNNNNNNGG 1 cut(s) 1253
BsmAI GTCTC 4 cut(s) 231, 426, 788, 1381
BsmFI GGGAC 1 cut(s) 1111
BsnI GGCC 1 cut(s) 857
Bsp119I TTCGAA 1 cut(s) 1269
Bsp1286I GDGCHC 2 cut(s) 110, 1585
Bsp13I TCCGGA 1 cut(s) 652
Bsp1407I TGTACA 1 cut(s) 1219
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 3 cut(s) 388, 1254, 1770
BspANI GGCC 1 cut(s) 857
BspCNI CTCAG 2 cut(s) 397, 892
BspDI ATCGAT 1 cut(s) 1517
BspEI TCCGGA 1 cut(s) 652
BspLI GGNNCC 2 cut(s) 972, 1638
BspMI ACCTGC 2 cut(s) 388, 408
BspT104I TTCGAA 1 cut(s) 1269
BsrDI GCAATG 3 cut(s) 797, 1021, 1543
BsrGI TGTACA 1 cut(s) 1219
BssECI CCNNGG 3 cut(s) 183, 858, 1680
BssT1I CCWWGG 2 cut(s) 858, 1680
Bst2UI CCWGG 2 cut(s) 1062, 1484
Bst4CI ACNGT 7 cut(s) 161, 498, 547, 588, 872, 1208, 1754
Bst6I CTCTTC 2 cut(s) 7, 1740
BstAPI GCANNNNNTGC 1 cut(s) 400
BstAUI TGTACA 1 cut(s) 1219
BstBI TTCGAA 1 cut(s) 1269
BstC8I GCNNGC 2 cut(s) 1299, 1585
BstDEI CTNAG 4 cut(s) 384, 900, 1131, 1775
BstF5I GGATG 3 cut(s) 232, 1035, 1322
BstMAI GTCTC 4 cut(s) 231, 426, 788, 1381
BstMWI GCNNNNNNNGC 3 cut(s) 400, 409, 1394
BstNI CCWGG 2 cut(s) 1062, 1484
BstSCI CCNGG 2 cut(s) 1060, 1482
BstV1I GCAGC 8 cut(s) 321, 403, 424, 814, 987, 1261, 1545, 1676
BstV2I GAAGAC 2 cut(s) 357, 745
BstX2I RGATCY 5 cut(s) 372, 769, 970, 1414, 1636
BstYI RGATCY 5 cut(s) 372, 769, 970, 1414, 1636
Bsu15I ATCGAT 1 cut(s) 1517
BsuI GTATCC 2 cut(s) 341, 535
BsuRI GGCC 1 cut(s) 857
BsuTUI ATCGAT 1 cut(s) 1517
BtsCI GGATG 3 cut(s) 232, 1035, 1322
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 2 cut(s) 690, 961
BveI ACCTGC 2 cut(s) 388, 408
Cac8I GCNNGC 2 cut(s) 1299, 1585
CaiI CAGNNNCTG 2 cut(s) 418, 1240
Cfr13I GGNCC 1 cut(s) 559
ClaI ATCGAT 1 cut(s) 1517
CseI GACGC 1 cut(s) 1377
Csp6I GTAC 4 cut(s) 1220, 1325, 1433, 1740
CviAII CATG 4 cut(s) 538, 1175, 1313, 1694
CviQI GTAC 4 cut(s) 1220, 1325, 1433, 1740
DdeI CTNAG 4 cut(s) 384, 900, 1131, 1775
DraIII CACNNNGTG 1 cut(s) 281
EaeI YGGCCR 1 cut(s) 855
Eam1104I CTCTTC 2 cut(s) 7, 1740
EarI CTCTTC 2 cut(s) 7, 1740
Ecl136II GAGCTC 2 cut(s) 108, 1583
Eco130I CCWWGG 2 cut(s) 858, 1680
Eco24I GRGCYC 2 cut(s) 110, 1585
Eco32I GATATC 1 cut(s) 454
Eco47I GGWCC 1 cut(s) 559
Eco53kI GAGCTC 2 cut(s) 108, 1583
Eco57I CTGAAG 3 cut(s) 54, 351, 515
EcoICRI GAGCTC 2 cut(s) 108, 1583
EcoRII CCWGG 2 cut(s) 1060, 1482
EcoRV GATATC 1 cut(s) 454
EcoT14I CCWWGG 2 cut(s) 858, 1680
EcoT38I GRGCYC 2 cut(s) 110, 1585
ErhI CCWWGG 2 cut(s) 858, 1680
FaeI CATG 4 cut(s) 541, 1178, 1316, 1697
FalI AAGNNNNNCTT 2 cut(s) 1653, 1685
FaqI GGGAC 1 cut(s) 1111
FatI CATG 4 cut(s) 537, 1174, 1312, 1693
FbaI TGATCA 2 cut(s) 139, 1621
Fnu4HI GCNGC 9 cut(s) 335, 389, 392, 413, 803, 1001, 1275, 1534, 1665
FokI GGATG 3 cut(s) 219, 1042, 1329
FriOI GRGCYC 2 cut(s) 110, 1585
Fsp4HI GCNGC 9 cut(s) 335, 389, 392, 413, 803, 1001, 1275, 1534, 1665
FspBI CTAG 5 cut(s) 47, 1004, 1053, 1101, 1562
GluI GCNGC 9 cut(s) 335, 389, 392, 413, 803, 1001, 1275, 1534, 1665
GsuI CTGGAG 3 cut(s) 204, 738, 1505
HaeIII GGCC 1 cut(s) 857
HapII CCGG 2 cut(s) 317, 653
HgaI GACGC 1 cut(s) 1377
Hin1II CATG 4 cut(s) 541, 1178, 1316, 1697
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 7 cut(s) 55, 204, 224, 299, 725, 754, 781
HpaII CCGG 2 cut(s) 317, 653
HphI GGTGA 4 cut(s) 255, 267, 369, 426
Hpy166II GTNNAC 2 cut(s) 1204, 1325
Hpy8I GTNNAC 2 cut(s) 1204, 1325
HpyAV CCTTC 5 cut(s) 127, 261, 362, 1346, 1490
HpyCH4III ACNGT 7 cut(s) 161, 498, 547, 588, 872, 1208, 1754
HpyCH4IV ACGT 1 cut(s) 1215
HpyF10VI GCNNNNNNNGC 3 cut(s) 400, 409, 1394
HpyF3I CTNAG 4 cut(s) 384, 900, 1131, 1775
HpySE526I ACGT 1 cut(s) 1215
Hsp92II CATG 4 cut(s) 541, 1178, 1316, 1697
Kpn2I TCCGGA 1 cut(s) 652
Ksp22I TGATCA 2 cut(s) 139, 1621
LmnI GCTCC 1 cut(s) 1486
Lsp1109I GCAGC 8 cut(s) 321, 403, 424, 814, 987, 1261, 1545, 1676
LweI GCATC 4 cut(s) 1020, 1509, 1596, 1699
MaeI CTAG 5 cut(s) 47, 1004, 1053, 1101, 1562
MaeII ACGT 1 cut(s) 1215
MaeIII GTNAC 4 cut(s) 64, 269, 726, 1242
MflI RGATCY 5 cut(s) 372, 769, 970, 1414, 1636
MhlI GDGCHC 2 cut(s) 110, 1585
MlsI TGGCCA 1 cut(s) 857
MluCI AATT 6 cut(s) 32, 466, 1383, 1490, 1598, 1648
MluNI TGGCCA 1 cut(s) 857
MlyI GAGTC 3 cut(s) 233, 734, 790
MmeI TCCRAC 2 cut(s) 325, 1614
Mox20I TGGCCA 1 cut(s) 857
MroI TCCGGA 1 cut(s) 652
MroXI GAANNNNTTC 2 cut(s) 468, 895
MscI TGGCCA 1 cut(s) 857
MseI TTAA 8 cut(s) 246, 480, 510, 665, 933, 1605, 1671, 1716
Msp20I TGGCCA 1 cut(s) 857
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 2 cut(s) 317, 653
MspR9I CCNGG 2 cut(s) 1062, 1484
MvaI CCWGG 2 cut(s) 1062, 1484
MwoI GCNNNNNNNGC 3 cut(s) 400, 409, 1394
NlaIII CATG 4 cut(s) 541, 1178, 1316, 1697
NlaIV GGNNCC 2 cut(s) 972, 1638
NmeAIII GCCGAG 1 cut(s) 602
NmuCI GTSAC 1 cut(s) 726
NspV TTCGAA 1 cut(s) 1269
PaqCI CACCTGC 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 1212
PdmI GAANNNNTTC 2 cut(s) 468, 895
PfeI GAWTC 4 cut(s) 55, 204, 299, 754
PfoI TCCNGGA 1 cut(s) 1482
PkrI GCNGC 9 cut(s) 336, 390, 393, 414, 804, 1002, 1276, 1535, 1666
PleI GAGTC 3 cut(s) 232, 733, 789
PpsI GAGTC 3 cut(s) 232, 733, 789
Psp124BI GAGCTC 2 cut(s) 110, 1585
Psp6I CCWGG 2 cut(s) 1060, 1482
PspGI CCWGG 2 cut(s) 1060, 1482
PspN4I GGNNCC 2 cut(s) 972, 1638
PspPI GGNCC 1 cut(s) 559
PstNI CAGNNNCTG 2 cut(s) 418, 1240
PsuI RGATCY 5 cut(s) 372, 769, 970, 1414, 1636
RsaI GTAC 4 cut(s) 1221, 1326, 1434, 1741
RsaNI GTAC 4 cut(s) 1220, 1325, 1433, 1740
SacI GAGCTC 2 cut(s) 110, 1585
SaqAI TTAA 8 cut(s) 246, 480, 510, 665, 933, 1605, 1671, 1716
SatI GCNGC 9 cut(s) 335, 389, 392, 413, 803, 1001, 1275, 1534, 1665
Sau96I GGNCC 1 cut(s) 559
SchI GAGTC 3 cut(s) 233, 734, 790
ScrFI CCNGG 2 cut(s) 1062, 1484
SduI GDGCHC 2 cut(s) 110, 1585
SfaNI GCATC 4 cut(s) 1020, 1509, 1596, 1699
SfuI TTCGAA 1 cut(s) 1269
SinI GGWCC 1 cut(s) 559
SmlI CTYRAG 2 cut(s) 109, 1410
SmoI CTYRAG 2 cut(s) 109, 1410
Sse9I AATT 6 cut(s) 32, 466, 1383, 1490, 1598, 1648
SsiI CCGC 3 cut(s) 388, 1254, 1770
SspI AATATT 1 cut(s) 894
SspMI CTAG 5 cut(s) 47, 1004, 1053, 1101, 1562
SstI GAGCTC 2 cut(s) 110, 1585
StyD4I CCNGG 2 cut(s) 1060, 1482
StyI CCWWGG 2 cut(s) 858, 1680
TaaI ACNGT 7 cut(s) 161, 498, 547, 588, 872, 1208, 1754
TaiI ACGT 1 cut(s) 1218
TaqI TCGA 6 cut(s) 312, 456, 567, 1269, 1517, 1781
TasI AATT 6 cut(s) 32, 466, 1383, 1490, 1598, 1648
TatI WGTACW 3 cut(s) 1219, 1324, 1432
TauI GCSGC 1 cut(s) 391
TfiI GAWTC 4 cut(s) 55, 204, 299, 754
Tru1I TTAA 8 cut(s) 246, 480, 510, 665, 933, 1605, 1671, 1716
Tru9I TTAA 8 cut(s) 246, 480, 510, 665, 933, 1605, 1671, 1716
TscAI CASTG 2 cut(s) 697, 961
TseFI GTSAC 1 cut(s) 726
TseI GCWGC 8 cut(s) 334, 391, 412, 802, 1000, 1274, 1533, 1664
Tsp45I GTSAC 1 cut(s) 726
TspDTI ATGAA 7 cut(s) 45, 165, 232, 642, 780, 1031, 1682
TspRI CASTG 2 cut(s) 697, 961
VpaK11BI GGWCC 1 cut(s) 559
XapI RAATTY 2 cut(s) 32, 466
XmnI GAANNNNTTC 2 cut(s) 468, 895
XspI CTAG 5 cut(s) 47, 1004, 1053, 1101, 1562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.